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Circtools2: Meta-data module #4
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Circtools2: Meta-data module #4
Commits on Nov 8, 2023
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Added CIRIquant command line arguments
shubhada-kulkarni committedNov 8, 2023 Configuration menu - View commit details
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Commits on Nov 9, 2023
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Added CIRiquant integration part in detect module
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Added cleanup string argument in detect module
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Changed the function to take arguments from detect class
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Added metatool part with command line checks and logging file printing
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Commits on Nov 14, 2023
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Fixed the duplicate -C option in detect module. Now replaced with -S
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Added nargs for list_ciriquant argument to remove parse arguments rea…
…ding error
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Commits on Nov 16, 2023
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Commit after making sure the testing worked successfully
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Fixed column names writing in merged output files in combining script
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Commented the comment deleted by mistake
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Commits on Nov 22, 2023
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Commits on Nov 28, 2023
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Making sure every run outputs same file using sorting of dictionary keys
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Script to run SLURM jobs for CIRIquant alignment
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Commits on Dec 6, 2023
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Added padlock probe module part
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Merge branch 'master' of https://github.com/shubhada-kulkarni/circtools
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Commits on Dec 7, 2023
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Commits on Dec 8, 2023
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Padlock directory with padlock.py script
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Commits on Dec 10, 2023
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Commits on Dec 11, 2023
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Added -GL input gene list file option to padlock probe module
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Input gene list file option added
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Removed dependency on R primer wrapper script
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Changes in BLAST step, running successfully
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Added function to calculate GC content
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Formatter R script for padlock probe designing
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Added the formatter part to write html outputs
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Commits on Dec 12, 2023
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Removed non-used command line parameters
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Removed non-used command line parameters from padlock module
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Commits on Dec 14, 2023
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Added flag for circular or linear RNA in padlock module
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Linear RNA flag added from circtools.py
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Commits on Dec 15, 2023
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Added missing gene names in the original function read_annotation_file
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Fixed the error in annotation_function for special entries
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Added exon extraction part for linear RNAs
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Added linear RNA scanning window part
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Code cleaning and adding linear temp exon file output
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Added full probe melting temperature criteria + code cleaning
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Commits on Dec 18, 2023
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Added BLAST offtarget search for linear probes
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Fixed all tmp files for linear RNA probes
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Commits on Dec 19, 2023
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Added HTML output for linear probes
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Commits on Dec 20, 2023
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Fixed multi genes output error for linear RNA probes
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Added BLAST step with command-line
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Commits on Jan 8, 2024
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Printing some additional columns for linear output to be consistent w…
…ith the HTML formatter script
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Adding full length melting temperature into output columns
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Changed ligation junction to strings from numbers
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Commits on Jan 10, 2024
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Merge branch 'master' of https://github.com/shubhada-kulkarni/circtools
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Added coordinates for linear HTML output
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Primer3 calling converted into a function
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Added rna_type flag conditions
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Commits on Jan 15, 2024
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Removed dependency on -d option for runs with linear RNAs only
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Commits on Jan 18, 2024
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Changed BLAST command to make consistent with command before
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Changed color steps for numbers to make coloring uniform
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Commits on Jan 19, 2024
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Changed BLAST to original python command
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Commits on Jan 24, 2024
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Changed melting temperature colors to red-yellow-green
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Changed BLAST back to local command
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Changed outputfile names and CSV headers
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Commits on Jan 25, 2024
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Changed steps to 8 and color palette to RdYlBu for colorblind-friendl…
…y colors
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Changed construct_color_column to take custom min and max values for …
…Tm and GC content
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Fixed linear coordinates gap in sequence + bed file for linear probes…
… generations
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Rounding of melting temperatures and GC content
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Changed palette to 11 colors + changed default GC to 35% + fixed bug …
…in TM_right column
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Changed palette back to 8 steps and GC percent to 40
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Commits on Jan 26, 2024
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Changed default exp name for padlock module
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Removed hover info from probe designsss
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Fixed the bug in Right_ column after previous change
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Added colour legends to HTML report
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Commits on Jan 29, 2024
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Added visualisation for circular probes
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Commits on Jan 30, 2024
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Re-added the hover-over for graphical representation
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Re-added the hover-over for graphical representation
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Modified svg filename for each circle
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Merge branch 'master' of https://github.com/shubhada-kulkarni/circtools
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Fixed the bug in the HTML output (was only printing one line)
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Fixed the font of RBD sequences from black to white
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Fixed the error after previous commit
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Commits on Jan 31, 2024
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Fixed linear FSJ CSV printing issue
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Temporarily turned off the melting temperature filtering criteria
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Commits on Feb 1, 2024
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Remove the havana annotation filter issue
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Fixed the background color issue when no hover over for sequence
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Commits on Feb 5, 2024
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Added the graphical representation part for circular RNAs
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Revert "Added the graphical representation part for circular RNAs"
This reverts commit 1805c67.
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Revert "Added the graphical representation part for circular RNAs"
This reverts commit 1805c67.
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Commits on Feb 6, 2024
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Fixed 1bp gap issue in start coordinates for circles
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Commits on Feb 7, 2024
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Fixed the merging exon bug for linear part
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Commits on Feb 8, 2024
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Added BED12 file writing for linear RNAs
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Commits on Feb 9, 2024
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Added first and last exon dictionary for circles (for BED12)
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Added 50bp fasta files for Xenium
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Commits on Feb 12, 2024
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Commits on Feb 13, 2024
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Added graphical representation function for circular RNAs
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Commits on Feb 14, 2024
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Added visualisation for linear probes
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Fixed the linear probe visualisation to one track
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Commits on Feb 15, 2024
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Fixed circles bed12 start position
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Corrected the FSJ probe graphical representation
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Commits on Feb 16, 2024
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Added -no-svg flag for graphical representation
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Added conditional formatting for probe representation based on no_svg…
… flag
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Added conditional SVG files generated based on no_svg flag
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Target fasta sequences for all FSJs
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Added target fasta writing with conditions
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Corrected reverse complementing for genes on - strand
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Fixed the RBD3 and RBD5 for circles on reverse strand
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Changed BLASTing individual probes to combined oligo BLAST
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Added hairpin, homo-heterodimer checks
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Merge branch 'master' of https://github.com/shubhada-kulkarni/circtools
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Added import of FetchData class
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Script to fetch and parse ortholog information using REST API
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Change function arguments to call from main wrapper script
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Added fetch ortho call + first-last exon info for circle
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Revert "Calling parse ortho funciton"
This reverts commit 149d2da.
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Class to fetch gene sequence from REST API
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Fixed the function description
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Modifications in function calling
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Removed unwanted function + added more tmp file in arguments
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Corrected the liftover function calling
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liftover command run + added parsing function
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Encapsulated the liftover binary call
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Added hg19 and mm10 flags to conservation part
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Added hg19 and mm10 flags and code to call liftover for these
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Added hg19 and mm10 check + conversion code
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Added part to call liftover on first and last exon
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Added function to fetch exon info from ensmbl
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Ortho dict info into liftover function
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Ortho dict + added function to parse GFF info from REST API
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Converted sequence fetching from gene to region
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Added target organism argument
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Added overlapping exon identification
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Added target species argument + fetch exon sequence function call
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Added try-exception for REST API requests
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Remaining API request count added
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Class function for sequence alignment
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Removed choices from target species (was causing errors)
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Added rat ID to dictionary + fixed the chain file name error
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Rearranged distance function + changed Align IO type to clustal
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Added multi-target species loop + fixed the target species ID input t…
…o functions
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Commits on Jun 18, 2024
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Added liftover case with no overlap with exon
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Commits on Jun 19, 2024
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Fixed bug for target species available choices if condition
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Added __main_condition for independent running
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Corrected first/BSJ temp liftover file names
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Commits on Jun 27, 2024
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Removed hard-coded species + added __main__ condition
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Removed y-axis labels and ticks
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Added genome versions to the plot
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Changed fasta header to contain circle coordinates for each species
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Added circle coordinate information function to the plot
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Commits on Jul 4, 2024
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Added function to perform pairwise alignments for all combinations wi…
…th source species
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Pairwise alignment flag option added
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Pairwise alignment if condition added
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Removed plot borders for phylogenetic plot
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Normalised the pairwise alignment score
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Check and skip for unlifted coordinates
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Added single-exon circle conditions
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Fixed coordinates and sequences for circles on negative strand
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Ortho gene found check + remove species if not
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Changed circle name for titles + increase phylo plot tick font
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