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update readme in progress
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sorelfitzgibbon committed Aug 2, 2023
1 parent b023a13 commit 5eb6aa7
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6 changes: 3 additions & 3 deletions docs/mutect2_flowchart.puml
Original file line number Diff line number Diff line change
Expand Up @@ -21,8 +21,8 @@ $intermediate_process(gatk_mergeVcfs, 'GATK MergeVcfs', 'Output: unfiltered VCF'
$intermediate_process(mutect2_mergeStats, 'GATK MergeMutectStats', 'Output: Mutect2 stats')
$intermediate_process(gatk_learnReadOrientation, 'GATK LearnReadOrientationModel', 'Output: orientation bias artifacts table')
$intermediate_process(mutect2_filterCalls, 'GATK FilterMutectCalls', 'Output: VCF with non-PASSing variants tagged %newline()Optional output: filtering stats')
$output_process(bcftools_filterVCF, 'remove non-PASS variants', 'Output: VCF including only variants that passed above filters')
$output_process(bcftools_splitVCF, 'split by variant type', 'Output: %newline()pass-snvs.vcf.gz %newline()pass-mnvs.vcf.gz %newline()pass-indels.vcf.gz')
$intermediate_process(bcftools_filterVCF, 'remove non-PASS variants', 'Output: VCF including only variants that passed above filters')
$output_process(bcftools_splitVCF, 'split by variant type', 'Output: %newline()SNV.vcf.gz %newline()MNV.vcf.gz %newline()Indel.vcf.gz')
$qc_process(gatk_filteringStats, 'Optional', 'Output: filteringStats.tsv')

reference_genome -d-> gatk_splitIntervals
Expand All @@ -47,7 +47,7 @@ reference_genome -d-> mutect2_filterCalls
contamination_table -d-> mutect2_filterCalls
mutect2_filterCalls -d-> gatk_filteringStats
mutect2_filterCalls -d-> bcftools_filterVCF : // filtered.vcf.gz //
bcftools_filterVCF -d-> bcftools_splitVCF : // pass.vcf.gz //
bcftools_filterVCF -d-> bcftools_splitVCF : // all-pass.vcf.gz //

$add_legend('left')

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2 changes: 1 addition & 1 deletion test/config/a_mini-all-tools.config
Original file line number Diff line number Diff line change
Expand Up @@ -17,7 +17,7 @@ params {
dataset_id = 'TWGSAMIN'
// setting params.exome to TRUE will add the '--exome' option when running manta and strelka2 and the -E option when running MuSE
exome = false
save_intermediate_files = false
save_intermediate_files = true

// module options
bgzip_extra_args = ''
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