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Geert van Geest committed Nov 24, 2023
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6 changes: 3 additions & 3 deletions 2023.11/day3/igv_visualisation/index.html
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Expand Up @@ -688,7 +688,7 @@ <h3 id="a-first-glance-the-e-coli-dataset">A first glance: the <em>E. coli</em>
<p><div class="highlight"><pre><span></span><code><span class="nb">cd</span><span class="w"> </span>~/project/results/alignments
samtools<span class="w"> </span>index<span class="w"> </span>SRR519926.sorted.region.bam
</code></pre></div>
Download it together with it&rsquo;s index file (<code>SRR519926.sorted.region.bam.bai</code>) and the reference genome (<code>ecoli-strK12-MG1655.fasta</code>) to your desktop.</p>
Download the alignment (<code>SRR519926.sorted.region.bam</code>) together with it&rsquo;s index file (<code>SRR519926.sorted.region.bam.bai</code>) and the reference genome (<code>ecoli-strK12-MG1655.fasta</code>) to your desktop.</p>
<div class="admonition note">
<p class="admonition-title">If working with Docker</p>
<p>If you are working with Docker, you can find the files in the working directory that you mounted to the docker container (with the <code>-v</code> option). So if you have used <code>-v C:\Users\myusername\ngs-course:/root/project</code>, your files will be in <code>C:\Users\myusername\ngs-course</code>.</p>
Expand Down Expand Up @@ -768,10 +768,10 @@ <h3 id="hcc1143-data-set">HCC1143 data set</h3>
<img src="../../assets/images/per_base.png" width="300"/>
</figure>

<p><strong>Exercise:</strong> What is the sequence coverage for that base? And the percentage T?</p>
<p><strong>Exercise:</strong> What is the sequence sequencing depth for that base? And the percentage T?</p>
<details class="done">
<summary>Answer</summary>
<p>The coverage is 62, and 25 reads (40%) T.</p>
<p>The depth is 62, and 25 reads (40%) T.</p>
</details>
<p>Navigate to region <code>chr21:19,800,320-19,818,162</code></p>
<p>Load repeat tracks by selecting <strong>File &gt; Load from Server&hellip;</strong> from the main menu and then select <strong>Annotations &gt; Variation and Repeats &gt; Repeat Masker</strong></p>
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