EEISP

EEISP identifies gene pairs that are codependent and mutually exclusive from single-cell RNA-seq data.

Installation

pip3 install eeisp

(Optional) Dependencies for GPU

EEISP requires cupy when using GPU computation --gpu. Use pip to install cupy like this (see the manual for more detail).

# For CUDA 9.2
pip3 install cupy-cuda92
# For CUDA 10.1
pip3 install cupy-cuda101

If you do not use --gpu, you do not need to install cupy.

Usage

EEISP takes a read count matrix as an input, in which rows and columns represent genes and cells, respectively. A gzipped file (.gz) is also acceptable.

0. (Optional) Convert CellRanger output to an input matrix (require R and Seurat library)

     datadir="outs/filtered_feature_bc_matrix/"
     matrix="matrix.txt"
     R -e "library(Seurat); so <- Read10X('$datadir'); write.table(so, '$matrix', quote=F, sep=',', col.names=T)"
   

1. eeisp calculates the CDI and EEI scores for all gene pairs. The output contains lists of gene pairs that have CDI or EEI values above the specified threshold and the tables of degree distribution.

     usage: eeisp [-h] [--threCDI THRECDI] [--threEEI THREEEI] [--tsv] [--gpu] [-p THREADS] [-v] matrix output
   
     positional arguments:
       matrix                Input matrix
       output                Output prefix
   
     optional arguments:
       -h, --help            show this help message and exit
       --threCDI THRECDI     Threshold for CDI (default: 20.0)
       --threEEI THREEEI     Threshold for EEI (default: 10.0)
       --tsv                 Specify when the input file is tab-delimited (.tsv)
       --gpu                 GPU mode
       -p THREADS, --threads THREADS  number of threads (default: 2)
       -v, --version         show program's version number and exit
   

2. eeisp_add_genename_from_geneid add Gene Names (Symbols) to the output files of eeisp.

    usage: eeisp_add_genename_from_geneid [-h] [--i_id I_ID] [--i_name I_NAME] input output genelist
   
    positional arguments:
      input            Input matrix
      output           Output prefix
      genelist         Gene list
   
    optional arguments:
      -h, --help       show this help message and exit
      --i_id I_ID      column number of gene id (default: 0)
      --i_name I_NAME  column number of gene name (default: 1)
   

Tutorial

The sample data is included in sample directory.

• data.txt: the input matrix of scRNA-seq data.
• genelidlist.txt: the gene list for eeisp_add_genename_from_geneid.

eeisp data.txt Sample --threCDI 0.5 --threEEI 0.5 -p 8

This command outputs gene pair lists that have CDI>0.5 or EEI>0.5. -p 8 means 8 CPUs are used.

Supply --gpu option to GPU computation (require cupy):

eeisp data.txt Sample --threCDI 0.5 --threEEI 0.5 -p 8 --gpu

(Note: Since GPU computation covers a part of eeisp, it is better to use multiple CPUs even in --gpu mode for the fast computation.)

Output files are:

   Sample_CDI_score_data_thre0.5.txt            # A list of gene pairs with CDI score.  
   Sample_CDI_degree_distribution_thre0.5.csv   # A table of the number of CDI degree and genes.  
   Sample_EEI_score_data_thre0.5.txt            # A list of gene pairs with EEI scores.  
   Sample_EEI_degree_distribution_thre0.5.csv   # A table of the number of EEI degree and genes.

The output files might include gene ids only.

   $ head Sample_CDI_score_data_thre0.5.txt
   2       7       ESG000003       ESG000008       0.96384320244841
   0       1       ESG000001       ESG000002       0.6852891560232545
   0       6       ESG000001       ESG000007       0.6852891560232545
   7       8       ESG000008       ESG000009       0.6852891560232545
   3       9       ESG000004       ESG000010       0.6469554204484568
   4       6       ESG100005       ESG000007       0.5258703930217091

If you want to add gene names (Symbols), use eeisp_add_genename_from_geneid with geneidlist.txt, which contains the pairs of gene ids and names.

 eeisp_add_genename_from_geneid \
     Sample_CDI_score_data_thre0.5.txt \
     Sample_CDI_score_data_thre0.5.addgenename.txt \
     geneidlist.txt
 eeisp_add_genename_from_geneid \
     Sample_EEI_score_data_thre0.5.txt \
     Sample_EEI_score_data_thre0.5.addgenename.txt \
     geneidlist.txt

The output files include gene names.

   $ head Sample_CDI_score_data_thre0.5.addgenename.txt
   2       7       ESG000003       ESG000008       OR4F5   FO538757.3      0.96384320244841
   0       1       ESG000001       ESG000002       RP11-34P13.3    FAM138A 0.6852891560232545
   0       6       ESG000001       ESG000007       RP11-34P13.3    RP11-34P13.9    0.6852891560232545
   7       8       ESG000008       ESG000009       FO538757.3      FO538757.2      0.6852891560232545
   3       9       ESG000004       ESG000010       RP11-34P13.7    AP006222.2      0.6469554204484568
   4       6       ESG100005       ESG000007       RP11-34P13.8    RP11-34P13.9    0.5258703930217091

Reference

Nakajima N., Hayashi T., Fujiki K., Shirahige K., Akiyama T., Akutsu T. and Nakato R., Codependency and mutual exclusivity for gene community detection from sparse single-cell transcriptome data, Nucleic Acids Research, 2021.