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A few tools I created to analyze my patch-clamp data

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This is a script package I developed to analyze my electrophysiological data. It is tailored to my patch-clamp protocols, but you may find some useful general methods here, which you'll probably have to modify in accordance with your data.

The package includes scripts to:

  • count action potentials in a series of current steps
  • extract some commonly analyzed properties from the first action potential at the rheobase
  • extract I/V curves from a series of voltage steps (includes a basic filter to remove spikes from the traces)
  • reliably find action potentials
  • calculate the membrane resistance using linear regression
  • calculate the resting membrane potential from a 0-pA current step
  • calculate the sag ratio from a hyperpolarizing current step
  • calculate the membrane capacitance and time constant according to Tamagnini et al., 2015, with modifications (I'm using a double exponential function and a -200-pA current step)

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A few tools I created to analyze my patch-clamp data

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