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updated README
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jr-leary7 committed Nov 23, 2023
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Expand All @@ -49,11 +49,11 @@ A quickstart guide on how to use `scLANE` with simulated data continues below, a

# Usage

Our method relies on a relatively simple test in order to define whether a given gene is differentially expressed (or "dynamic") over the provided trajectory. While the exact structure of the test differs by model backend, the concept is the same: the spline-based NB GLM / GEE / GLMM is treated as the alternate model, and a null model is fit using the corresponding model backend. If the GLM backend is used, then the null model is simply an intercept-only NB GLM; the GEE backend fits an intercept-only model with the same working correlation structure as the alternate model, and if the GLMM backend is used then the null model is an intercept-only model with random intercepts for each subject. The alternate hypothesis is thus that at least one of the estimated coefficients is significantly different from zero. We predict a given gene to be dynamic if the adjusted *p*-value of the test is less than an *a priori* threshold; the default threshold is 0.01, and the default adjustment method is [the Holm correction](https://en.wikipedia.org/wiki/Holm–Bonferroni_method).
Our method relies on a relatively simple test in order to define whether a given gene is differentially expressed (or "dynamic") over the provided trajectory. While the exact structure of the test differs by model backend, the concept is the same: the spline-based NB GLM / GEE / GLMM is treated as the alternate model, and a null model is fit using the corresponding model backend. If the GLM backend is used, then the null model is simply an intercept-only NB GLM; the GEE backend fits an intercept-only model with the same working correlation structure as the alternate model, and if the GLMM backend is used then the null model is an intercept-only model with random intercepts for each subject. The alternate hypothesis is thus that at least one of the estimated coefficients is significantly different from zero. We predict a given gene to be dynamic if the adjusted *p*-value of the test is less than an *a priori* threshold; the default threshold is $\alpha = 0.01$, and the default adjustment method is [the Holm correction](https://en.wikipedia.org/wiki/Holm–Bonferroni_method).

## Libraries

First we'll also need to load a couple dependencies & resolve a function conflict.
First we'll need to load a couple packages.

```{r libraries, results='hide', message=FALSE}
library(dplyr)
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