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| name: Map NCBI and Ensembl files | |
| on: | |
| push: | |
| branches: [ "main" ] | |
| workflow_dispatch: | |
| jobs: | |
| map-files: | |
| runs-on: ubuntu-latest | |
| steps: | |
| - name: Checkout code | |
| uses: actions/checkout@v3 | |
| - name: Install Dependencies | |
| run: | | |
| curl -O 'https://ftp.ncbi.nlm.nih.gov/pub/datasets/command-line/v2/linux-amd64/dataformat' | |
| curl -O 'https://ftp.ncbi.nlm.nih.gov/pub/datasets/command-line/v2/linux-amd64/datasets' | |
| chmod +x datasets dataformat | |
| - name: Set Environment Variables | |
| run: | | |
| #datasets | |
| echo "FIELDS=accession,assminfo-name,organism-name,organism-tax-id,annotinfo-name,annotinfo-method,annotinfo-featcount-gene-total,annotinfo-pipeline,annotinfo-provider,annotinfo-release-date" >> $GITHUB_ENV | |
| #input files | |
| echo "NCBI=ncbi.tsv" >> $GITHUB_ENV | |
| echo "ENSEMBL_RR=ensembl_rapid_release.tsv" >> $GITHUB_ENV | |
| #output file | |
| echo "MERGED_TABLE=merged_table.tsv" >> $GITHUB_ENV | |
| echo "OUTPUT=mapped_annotations.tsv" >> $GITHUB_ENV | |
| - name: Collect Data from TSVs | |
| run: | | |
| # Define the input files (two TSVs) and output file | |
| input1="${{env.NCBI}}" | |
| input2="${{env.ENSEMBL_RR}}" | |
| output="${{env.MERGED_TABLE}}" | |
| # Merge two TSVs, process columns, and save to output | |
| { tail -n +2 "$input1"; tail -n +2 "$input2"; } | awk -F'\t' '{ | |
| # Get the accession (first column) | |
| accession = $1; | |
| # Extract the filename from the full path (second column), remove .gff.gz or .gff3.gz extension | |
| match($2, /([^\/]+)\.(gff3?|gff)\.gz$/, arr); | |
| filename = arr[1]; | |
| # Print accession, filename without extension, and full URL (second column) | |
| print accession "\t" filename "\t" $2; | |
| }' > "$output" | |
| # Show the output file content for debugging purposes | |
| cat "$output" | |
| - name: Get NCBI Assemblies Metadata | |
| run: | | |
| tmp=tmp.txt | |
| cat "${{env.MERGED_TABLE}}" | awk -F'\t' '{print $1}' > "$tmp" | |
| ./datasets summary genome genome --inputfile "$tmp" --as-json-lines | | |
| ./dataformat tsv genome --fields "${{env.FIELDS}}" | | |
| tail -n +2 | head -n 10 > "${{env.NCBI_ACCESSIONS}}" | |
| cat "${{env.NCBI_ACCESSIONS}}" |