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Reclassifying Results

Robert J. Gifford edited this page Jun 24, 2024 · 1 revision

Inspecting screening results often reveals the deficiencies of the reference sequence library that was used to classify screening results.

In particular, screening can make it clear that there are certain variants of
the genome feature being investigated that are not represented in the reference sequence library.

When this happens it is useful to reclassify sequences identified via screening using an updated reference sequence library that includes representatives of the missing variant(s).

To do this, simply add the new sequences to your project-specific reference library and run the digs_tool.pl script using your project control file and the -m=3 option, as shown here:

./digs_tool.pl -m=3 -i ../projects/eve/erv_1_lenti.ctl 


Connecting to DB:  erv_lenti
Created report directory
Path: './tmp/result_set_33636_1618435124'
Reference library: 59 amino acid sequences


Enter a WHERE statement to limit reaasign (Optional) : WHERE assigned_name = 'RELIK'

The option to limit the reassign via a WHERE statement is presented. This can save time when the aim is to reassign only a subset of hits in a digs_results table with a large number of entries. If using a WHERE statement enter it as you would in a SQL client (i.e. using appropriate syntax), as shown above.