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W22.Protein.Protein.Interactions

This is the home page for the Collaboratory Workshop, Introduction to Protein Protein Interactions and Cytoscape.

Slide Handouts and Schedule [Legacy Handouts]

  • Day 1 [Handout]:

    • Experimental Methods

      • Experiment uncertainties

        • Ambiguous interaction types
        • Ambiguous protein identification
      • Database Record Contents

        • Binary expansion
      • Database Record Formats

        • PSI-MI MIF/MITAB
        • BioGRID
        • Cytoscape (.sif)
      • Protein Interaction Resources

        • Primary Databases
        • Integrators
        • Web Services
  • Day 2 [Handout]:

    • Interaction Data Processing

      • PSI-MI MIF Format

        • Record structure
        • Compact vs expanded form
      • PSI-MI Controlled Vocabulary

      • Parsing XML Files

        • Python lxml toolkit
      • SIF (Cytoscape) Format

      • MIF to SIF conversion

  • Day 3 [Handout]:

    • Introduction to Cytoscape

      • Interaction Networks

      • Cytoscape Overview

        • Installation
        • Basic features
        • Apps/Plugins overview
      • Loading Interaction Data

        • Local files
        • Public resources
        • Network Annotation
      • Basic Network Visualization

        • Node/Edge Attribute Mapping

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