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A workbench for the translational control of gene expression

This repository contains the codes used to compare the ORF-specific Ribo-seq profiles coming from different datasets and to assess the reproducibility of them.

Our analysis of the ORF-specific Ribo-seq profiles consist of two phases:

Upstream phase:

The upstream phase allows us to compute the Ribo-seq profiles starting from the raw Ribo-seq data

Downstream phase:

The downstream phase is the core of our method and is articulated as follows:

  • Set up of the coverage matrix and elaboration of digitalised profiles

  • Comparison of the digitalised profiles

  • Assessment of the similarity scores

  • Identification of the "significantly reproducible Ribo-seq profiles"