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Multi-omic #25

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zhangqc723 opened this issue May 30, 2023 · 1 comment
Open

Multi-omic #25

zhangqc723 opened this issue May 30, 2023 · 1 comment

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@zhangqc723
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Hello,
Now, I had got the output form 10X Multi-omic (RNA+ATAC) by cellranger-arc, and I want to use velocyto run10x for my RNA data form 10X RNA+ATAC. What should I do?
Thanks
@danielee0707
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danielee0707 commented May 31, 2023 •
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Please follow Velocyto's documentation to run their CLI tool. See http://velocyto.org/velocyto.py/tutorial/cli.html#run10x-run-on-10x-chromium-samples.

If that doesn't work on Multiome, you can also try directly calling Velocyto run http://velocyto.org/velocyto.py/tutorial/cli.html#run-run-on-any-technique-advanced-use using the 10X barcode file for --bcfile argument and gex_possorted_bam.bam as the input BAM file.

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@danielee0707 @zhangqc723