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Add VEP annotation via subworkflow #21

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@famosab famosab commented Nov 25, 2024

PR checklist

  • This comment contains a description of changes (with reason).
  • If you've fixed a bug or added code that should be tested, add tests!
  • If you've added a new tool - have you followed the pipeline conventions in the contribution docs
  • Make sure your code lints (nf-core lint).
  • Ensure the test suite passes (nextflow run . -profile test,docker --outdir <OUTDIR>).
  • Check for unexpected warnings in debug mode (nextflow run . -profile debug,test,docker --outdir <OUTDIR>).
  • Usage Documentation in docs/usage.md is updated.
  • Output Documentation in docs/output.md is updated.
  • CHANGELOG.md is updated.
  • README.md is updated (including new tool citations and authors/contributors).

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github-actions bot commented Nov 25, 2024

nf-core pipelines lint overall result: Failed ❌

Posted for pipeline commit 553fb29

+| ✅ 157 tests passed       |+
#| ❔  22 tests were ignored |#
!| ❗   9 tests had warnings |!
-| ❌   5 tests failed       |-

❌ Test failures:

  • schema_params - Param vep_cache from nextflow config not found in nextflow_schema.json
  • schema_params - Param run_vep from nextflow config not found in nextflow_schema.json
  • schema_params - Param vep_cache_version from nextflow config not found in nextflow_schema.json
  • schema_params - Param vep_genome from nextflow config not found in nextflow_schema.json
  • schema_params - Param vep_species from nextflow config not found in nextflow_schema.json

❗ Test warnings:

  • nextflow_config - Config variable not found: validation.help.beforeText
  • nextflow_config - Config variable not found: validation.help.afterText
  • nextflow_config - Config variable not found: validation.summary.beforeText
  • nextflow_config - Config variable not found: validation.summary.afterText
  • nextflow_config - Config manifest.version should end in dev: 1.1.0
  • pipeline_todos - TODO string in README.md: Add bibliography of tools and data used in your pipeline
  • pipeline_todos - TODO string in main.nf: Optionally add in-text citation tools to this list.
  • pipeline_todos - TODO string in main.nf: Optionally add bibliographic entries to this list.
  • pipeline_todos - TODO string in main.nf: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled!

❔ Tests ignored:

  • files_exist - File is ignored: CODE_OF_CONDUCT.md
  • files_exist - File is ignored: assets/nf-core-vcftomaf_logo_light.png
  • files_exist - File is ignored: docs/images/nf-core-vcftomaf_logo_light.png
  • files_exist - File is ignored: docs/images/nf-core-vcftomaf_logo_dark.png
  • files_exist - File is ignored: .github/ISSUE_TEMPLATE/config.yml
  • files_exist - File is ignored: .github/workflows/awstest.yml
  • files_exist - File is ignored: .github/workflows/awsfulltest.yml
  • files_exist - File is ignored: conf/test_full.config
  • nextflow_config - Config variable ignored: manifest.name
  • nextflow_config - Config variable ignored: manifest.homePage
  • files_unchanged - File ignored due to lint config: CODE_OF_CONDUCT.md
  • files_unchanged - File ignored due to lint config: .github/CONTRIBUTING.md
  • files_unchanged - File ignored due to lint config: .github/ISSUE_TEMPLATE/bug_report.yml
  • files_unchanged - File does not exist: .github/ISSUE_TEMPLATE/config.yml
  • files_unchanged - File ignored due to lint config: .github/PULL_REQUEST_TEMPLATE.md
  • files_unchanged - File ignored due to lint config: assets/email_template.txt
  • files_unchanged - File ignored due to lint config: assets/sendmail_template.txt
  • files_unchanged - File ignored due to lint config: assets/nf-core-vcftomaf_logo_light.png
  • files_unchanged - File ignored due to lint config: docs/images/nf-core-vcftomaf_logo_light.png
  • files_unchanged - File ignored due to lint config: docs/images/nf-core-vcftomaf_logo_dark.png
  • files_unchanged - File ignored due to lint config: docs/README.md
  • actions_awstest - 'awstest.yml' workflow not found: /home/runner/work/vcftomaf/vcftomaf/.github/workflows/awstest.yml

✅ Tests passed:

Run details

  • nf-core/tools version 3.0.2
  • Run at 2024-11-25 11:48:17

@famosab famosab linked an issue Nov 25, 2024 that may be closed by this pull request
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famosab commented Nov 26, 2024

Problem so far: The index file is older than the vcf.gz file...

executor >  local (6)
[53/d41436] process > QBICPIPELINES_VCFTOMAF:VCFTOMAF:UNTAR (vep.tar.gz)     [100%] 1 of 1 ✔
[d1/ad6aac] process > QBICPIPELINES_VCFTOMAF:VCFTOMAF:ENSEMBLVEP_VEP (test2) [100%] 2 of 2 ✔
[0c/80a19f] process > QBICPIPELINES_VCFTOMAF:VCFTOMAF:TABIX_TABIX (test2)    [100%] 2 of 2 ✔
[7a/b3cb1c] process > QBICPIPELINES_VCFTOMAF:VCFTOMAF:BCFTOOLS_VIEW (test)   [ 50%] 1 of 2, failed: 1
[-        ] process > QBICPIPELINES_VCFTOMAF:VCFTOMAF:GUNZIP                 -
[-        ] process > QBICPIPELINES_VCFTOMAF:VCFTOMAF:VCF2MAF                -
[-        ] process > QBICPIPELINES_VCFTOMAF:VCFTOMAF:MULTIQC                -
[DUMP: vcf-before] [['id':'test', 'index':true, 'normal_id':'testN', 'vcf_normal_id':'testN'], /home/paifb01/vcftomaf/work/80/5d0858f352f14c963373766c511701/test.vcf.gz]
[DUMP: vcf-sfter] [['id':'test', 'index':true, 'normal_id':'testN', 'vcf_normal_id':'testN'], /home/paifb01/vcftomaf/work/80/5d0858f352f14c963373766c511701/test.vcf.gz, /home/paifb01/vcftomaf/work/81/bbdcd6d89e3166b3629350da6aea6e/test.vcf.gz.tbi]
[DUMP: vcf-before] [['id':'test2', 'index':false, 'tumor_id':'testT', 'vcf_tumor_id':'testT'], /home/paifb01/vcftomaf/work/d1/ad6aacd5607f07379d37d7619767f5/test2.vcf.gz]
[DUMP: vcf-sfter] [['id':'test2', 'index':false, 'tumor_id':'testT', 'vcf_tumor_id':'testT'], /home/paifb01/vcftomaf/work/d1/ad6aacd5607f07379d37d7619767f5/test2.vcf.gz, /home/paifb01/vcftomaf/work/0c/80a19f8f4a5a74ae77648d7ff60263/test2.vcf.gz.tbi]
Execution cancelled -- Finishing pending tasks before exit
-[qbic-pipelines/vcftomaf] Pipeline completed with errors-
ERROR ~ Error executing process > 'QBICPIPELINES_VCFTOMAF:VCFTOMAF:BCFTOOLS_VIEW (test)'

Caused by:
  Process `QBICPIPELINES_VCFTOMAF:VCFTOMAF:BCFTOOLS_VIEW (test)` terminated with an error exit status (9)


Command executed:

  bcftools view \
      --output test.vcf.gz \
      --regions-file genome.bed \
       \
       \
      --output-type z \
      --threads 4 \
      test.vcf.gz
  
  cat <<-END_VERSIONS > versions.yml
  "QBICPIPELINES_VCFTOMAF:VCFTOMAF:BCFTOOLS_VIEW":
      bcftools: $(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*$//')
  END_VERSIONS

Command exit status:
  9

Command output:
  (empty)

Command error:
  Error: BCF read error

Work dir:
  /home/paifb01/vcftomaf/work/7a/b3cb1cf4b5c49a62635b8e8284521c

Container:
  quay.io/biocontainers/bcftools:1.20--h8b25389_0

Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run`

 -- Check '.nextflow.log' file for details
ERROR ~ Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting

 -- Check '.nextflow.log' file for details

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Vcf2Maf currently doens't work with VEP annotation
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