diff --git a/CHANGELOG.md b/CHANGELOG.md
index 479077cfbde..f77597142e8 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -11,6 +11,7 @@ This project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.htm
 ### Added
 
 - Add the new `go.opentelemetry.io/contrib/instrgen` package to provide auto-generated source code instrumentation. (#3068, #3108)
+- Add `WithSpanStartOption` and `WithSpanEndOption` to the `go.opentelemetry.io/contrib/github.com/gorilla/mux/otelmux` package to provide options on span start and end. (#5250)
 
 ### Removed
 
diff --git a/instrumentation/github.com/gorilla/mux/otelmux/config.go b/instrumentation/github.com/gorilla/mux/otelmux/config.go
index 2900924838b..c8ae1401f12 100644
--- a/instrumentation/github.com/gorilla/mux/otelmux/config.go
+++ b/instrumentation/github.com/gorilla/mux/otelmux/config.go
@@ -18,6 +18,8 @@ type config struct {
 	PublicEndpoint    bool
 	PublicEndpointFn  func(*http.Request) bool
 	Filters           []Filter
+	SpanStartOptions  []oteltrace.SpanStartOption
+	SpanEndOptions    []oteltrace.SpanEndOption
 }
 
 // Option specifies instrumentation configuration options.
@@ -86,6 +88,22 @@ func WithSpanNameFormatter(fn func(routeName string, r *http.Request) string) Op
 	})
 }
 
+// WithSpanStartOption applies options to all the HTTP span created by the
+// instrumentation.
+func WithSpanStartOption(o ...oteltrace.SpanStartOption) Option {
+	return optionFunc(func(c *config) {
+		c.SpanStartOptions = append(c.SpanStartOptions, o...)
+	})
+}
+
+// WithSpanEndOption applies options when ending the HTTP span created by the
+// instrumentation.
+func WithSpanEndOption(o ...oteltrace.SpanEndOption) Option {
+	return optionFunc(func(c *config) {
+		c.SpanEndOptions = append(c.SpanEndOptions, o...)
+	})
+}
+
 // WithFilter adds a filter to the list of filters used by the handler.
 // If any filter indicates to exclude a request then the request will not be
 // traced. All filters must allow a request to be traced for a Span to be created.
diff --git a/instrumentation/github.com/gorilla/mux/otelmux/mux.go b/instrumentation/github.com/gorilla/mux/otelmux/mux.go
index c7b2355eca8..a973f39310f 100644
--- a/instrumentation/github.com/gorilla/mux/otelmux/mux.go
+++ b/instrumentation/github.com/gorilla/mux/otelmux/mux.go
@@ -55,6 +55,8 @@ func Middleware(service string, opts ...Option) mux.MiddlewareFunc {
 			publicEndpoint:    cfg.PublicEndpoint,
 			publicEndpointFn:  cfg.PublicEndpointFn,
 			filters:           cfg.Filters,
+			SpanStartOptions:  cfg.SpanStartOptions,
+			SpanEndOptions:    cfg.SpanEndOptions,
 		}
 	}
 }
@@ -68,6 +70,8 @@ type traceware struct {
 	publicEndpoint    bool
 	publicEndpointFn  func(*http.Request) bool
 	filters           []Filter
+	SpanStartOptions  []trace.SpanStartOption
+	SpanEndOptions    []trace.SpanEndOption
 }
 
 type recordingResponseWriter struct {
@@ -141,10 +145,10 @@ func (tw traceware) ServeHTTP(w http.ResponseWriter, r *http.Request) {
 		}
 	}
 
-	opts := []trace.SpanStartOption{
+	opts := append(tw.SpanStartOptions, []trace.SpanStartOption{
 		trace.WithAttributes(semconvutil.HTTPServerRequest(tw.service, r)...),
 		trace.WithSpanKind(trace.SpanKindServer),
-	}
+	}...)
 
 	if tw.publicEndpoint || (tw.publicEndpointFn != nil && tw.publicEndpointFn(r.WithContext(ctx))) {
 		opts = append(opts, trace.WithNewRoot())
@@ -162,7 +166,7 @@ func (tw traceware) ServeHTTP(w http.ResponseWriter, r *http.Request) {
 	}
 	spanName := tw.spanNameFormatter(routeStr, r)
 	ctx, span := tw.tracer.Start(ctx, spanName, opts...)
-	defer span.End()
+	defer span.End(tw.SpanEndOptions...)
 	r2 := r.WithContext(ctx)
 	rrw := getRRW(w)
 	defer putRRW(rrw)
diff --git a/instrumentation/github.com/gorilla/mux/otelmux/test/mux_test.go b/instrumentation/github.com/gorilla/mux/otelmux/test/mux_test.go
index 13e900b5ca1..108bf4fb326 100644
--- a/instrumentation/github.com/gorilla/mux/otelmux/test/mux_test.go
+++ b/instrumentation/github.com/gorilla/mux/otelmux/test/mux_test.go
@@ -9,6 +9,7 @@ import (
 	"net/http"
 	"net/http/httptest"
 	"testing"
+	"time"
 
 	"github.com/gorilla/mux"
 	"github.com/stretchr/testify/assert"
@@ -282,3 +283,67 @@ func TestWithPublicEndpointFn(t *testing.T) {
 		})
 	}
 }
+
+func TestWithSpanStartOptions(t *testing.T) {
+	sr := tracetest.NewSpanRecorder()
+	provider := sdktrace.NewTracerProvider(sdktrace.WithSpanProcessor(sr))
+
+	// Setup
+	router := mux.NewRouter()
+	router.Use(otelmux.Middleware("foobar",
+		otelmux.WithTracerProvider(provider),
+		otelmux.WithSpanStartOption(
+			trace.WithAttributes(attribute.String("spanStart", "true")),
+		),
+	))
+
+	// Configure an empty handler
+	router.HandleFunc("/", func(writer http.ResponseWriter, request *http.Request) {
+	})
+	r := httptest.NewRequest("GET", "/", nil)
+	w := httptest.NewRecorder()
+	router.ServeHTTP(w, r)
+	response := w.Result()
+	assert.Equal(t, http.StatusOK, response.StatusCode)
+
+	// Verify that the attribute is set as expected
+	spans := sr.Ended()
+	require.Len(t, spans, 1)
+	span := spans[0]
+	assert.Equal(t, "/", span.Name())
+	attr := span.Attributes()
+	assert.Contains(t, attr, attribute.String("spanStart", "true"))
+}
+
+func TestWithSpanEndOptions(t *testing.T) {
+	sr := tracetest.NewSpanRecorder()
+	provider := sdktrace.NewTracerProvider(sdktrace.WithSpanProcessor(sr))
+
+	// Setup
+	router := mux.NewRouter()
+	endTime := time.Now()
+	router.Use(otelmux.Middleware("foobar",
+		otelmux.WithTracerProvider(provider),
+		otelmux.WithSpanEndOption(
+			trace.WithTimestamp(endTime),
+		),
+	))
+
+	// Configure an empty handler
+	router.HandleFunc("/", func(writer http.ResponseWriter, request *http.Request) {
+	})
+	r := httptest.NewRequest("GET", "/", nil)
+	w := httptest.NewRecorder()
+	router.ServeHTTP(w, r)
+	response := w.Result()
+	assert.Equal(t, http.StatusOK, response.StatusCode)
+
+	// Verify that the attribute is set as expected
+	spans := sr.Ended()
+	require.Len(t, spans, 1)
+	span := spans[0]
+	assert.Equal(t, "/", span.Name())
+
+	// Assert that the time set in the SpanEndOptions above matches the EndTime of the span
+	assert.Equal(t, span.EndTime(), endTime)
+}