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I don't know if this is correct, I looked at the output file "segmentation.csv" and found that the cell column value is NaN, can you help me find this error?
Best,
Jun
The text was updated successfully, but these errors were encountered:
Hello!
Thank you for the tool. I am interested in trying this tool on my own data(Stereo-seq data['geneID', 'x', 'y', 'MIDCounts']).
baysor run ./stereo-seq.csv -p -c ./configs/example_config.toml -p -o ./stereo-seq_output_no_dapi/ -m 1 -g 'geneID' -s 8
[17:11:06] Info: Run R1b9f25c4f
[17:11:06] Info: (2023-09-15) Run Baysor v0.6.2
[17:11:06] Info: Loading data...
[17:11:08] Info: Loaded 138957 transcripts
[17:11:10] Info: Estimating noise level
[17:11:16] Info: Done
[17:11:29] Info: Clustering molecules...
Progress: 100%|███████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████| Time: 0:00:02
Iteration: 66
Max. difference: 9.26e-5
Fraction of probs changed: 0.00238
[17:12:51] Info: Algorithm stopped after 66 iterations. Error: 9.26e-5. Converged: true.
[17:12:51] Info: Done
[17:12:51] Info: Initializing algorithm. Scale: 8.0, scale std: 2.0, initial #components: 92638, #molecules: 138957.
[17:12:51] Warning: n=92638, which is > length(high_conf_ids) (2553)
└ Baysor.Processing /home/viktor_petukhov/.julia/dev/Baysor/src/processing/data_processing/initialization.jl:180
[17:12:52] Info: Using the following additional information about molecules: [:confidence, :cluster]
[17:12:52] Info: Using 2D coordinates
Progress: 100%|███████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████| Time: 0:03:45
Iteration: 500
Noise level, %: 98.17
Num. components: 319
[17:16:39] Info: Processing complete.
[17:16:39] Info: Estimating local colors
[17:18:42] Warning: n=10000, which is > length(high_conf_ids) (2553)
└ Baysor.Processing /home/viktor_petukhov/.julia/dev/Baysor/src/processing/data_processing/initialization.jl:180
[17:24:19] Info: Estimating boundary polygons
[17:24:20] Info: Saving results to ./stereo-seq_output_no_dapi/segmentation.csv
Progress: 100%|███████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████| Time: 0:00:07
[17:24:35] Info: Plotting results
[17:24:37] Info: Plot diagnostics
[17:24:39] Info: Plot transcript assignment
[17:24:46] Info: All done!
(process:3486428): GLib-CRITICAL **: 17:24:46.069: g_datalist_id_set_data_full: assertion 'key_id > 0' failed
(process:3486428): GLib-CRITICAL **: 17:24:46.069: g_datalist_id_set_data_full: assertion 'key_id > 0' failed
(process:3486428): GLib-CRITICAL **: 17:24:46.070: g_datalist_id_set_data_full: assertion 'key_id > 0' failed
I don't know if this is correct, I looked at the output file "segmentation.csv" and found that the cell column value is NaN, can you help me find this error?
Best,
Jun
The text was updated successfully, but these errors were encountered: