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update description of metajunction plot
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pre-mRNA committed Nov 8, 2024
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10 changes: 6 additions & 4 deletions README.md
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Expand Up @@ -125,10 +125,13 @@ We can also study the distribution of m6A sites around exon-exon junctions in an
$ r2d plotMetaJunction -i "./test/annotate.bed" -o "./test/metajunction_m6A.png" -f "fraction_modified" -u "10" -t "upper"
```

The plot shows the proportion of sites that have greater than 10% m6A methylation, with their distances to local splice-junctions:
The plot shows the proportion of sites that have greater than 10% m6A methylation, and their distances to local splice-junctions:

Distances to downstream junctions are indicated on the right side of the plot. Distances to upstream junctions are indicated on the left side of the plot.

![Distribution of m6A sites around exon junctions](./assets/metajunction_m6A.png)

More information about R2Dtool plots is available on the [R2Dtool Wiki](https://github.com/comprna/R2Dtool/wiki/Visualising-RNA-feature-distributions-with-R2Dtool.
More information about R2Dtool plots is available on the [R2Dtool Wiki](https://github.com/comprna/R2Dtool/wiki/Visualising-RNA-feature-distributions-with-R2Dtool).


# R2Dtool file formats
Expand Down Expand Up @@ -297,8 +300,7 @@ Optional arguments:
- The flag "-o [/path/to/table.tsv]" can be used to save the aggregated metajunction data as a tab-separated file
```

More information on ```r2d``` plot functions can be found on the [R2Dtool wiki pages](
https://github.com/comprna/R2Dtool/wiki/Visualising-RNA-feature-distributions-with-R2Dtool)
More information on ```r2d``` plot functions can be found on the [R2Dtool wiki pages](https://github.com/comprna/R2Dtool/wiki/Visualising-RNA-feature-distributions-with-R2Dtool)



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Binary file modified assets/metajunction_m6A.png
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