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[ascatNgs](https://github.com/cancerit/ascatNgs/wiki) * [VAGrENT](https://github.com/cancerit/VAGrENT/wiki) +## Diagram of internals + +This diagram was generated based on v1.1.0, it does not describe any of the file provisioning handled by a Dockstore run. + +![Internal flow of docker image](images/dockstore-cgpwgs.png) + Release process =============== This project is maintained using HubFlow. From 3f83502eb022019036ef28afa5238914593d1dda Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Mon, 12 Feb 2018 13:29:37 +0000 Subject: [PATCH 03/57] Handle inadequacy of Bio::DB::HTS by creating a local cache [ci skip] --- scripts/analysisWGS.sh | 9 +++++++++ 1 file changed, 9 insertions(+) diff --git a/scripts/analysisWGS.sh b/scripts/analysisWGS.sh index f5625f0..efc0cd9 100755 --- a/scripts/analysisWGS.sh +++ b/scripts/analysisWGS.sh @@ -123,6 +123,12 @@ perl -alne 'print join(qq{\t},$F[0],0,$F[1],2);' < $REF_BASE/genome.fa.fai | tee echo "Setting up Parallel block 1" +## prime the cache +USER_CACHE=$OUTPUT_DIR/ref_cache +export REF_CACHE=$USER_CACHE/%2s/%2s/%s +export REF_PATH=$REF_CACHE:http://www.ebi.ac.uk/ena/cram/md5/%s +do_parallel[cache_POP]="seq_cache_populate.pl -root $USER_CACHE $REF_BASE/genome.fa" + echo -e "\t[Parallel block 1] CaVEMan setup added..." do_parallel[CaVEMan_setup]="caveman.pl \ -r $REF_BASE/genome.fa.fai \ @@ -425,6 +431,9 @@ rm -f $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/battenberg/tmpBattenberg/ mv $OUTPUT_DIR/timings/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.time.verify_WT $OUTPUT_DIR/timings/${PROTOCOL}_${NAME_WT}.time.verify_WT mv $OUTPUT_DIR/timings/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.time.verify_MT $OUTPUT_DIR/timings/${PROTOCOL}_${NAME_MT}.time.verify_MT +# cleanup ref cache +rm -rf $USER_CACHE + echo 'Package results' # timings first tar -C $OUTPUT_DIR -zcf ${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.timings.tar.gz timings From 2a6c681570f23d6b425a09bfd9e7788d2f6c63c2 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Mon, 12 Feb 2018 16:34:52 +0000 Subject: [PATCH 04/57] First pass at reorg for future proofing [ci skip] --- CHANGES.md | 45 +++-- Dockerfile | 83 +++++++-- Dockstore.cwl | 165 +----------------- build/apt-build.sh | 19 -- build/perllib-build.sh | 5 - cwls/cgpwgs.cwl | 164 +++++++++++++++++ .../bam_bai.json} | 0 scripts/{ds-wrapper.pl => ds-cgpwgs.pl} | 0 8 files changed, 264 insertions(+), 217 deletions(-) mode change 100644 => 120000 Dockstore.cwl delete mode 100644 build/apt-build.sh delete mode 100644 build/perllib-build.sh create mode 100644 cwls/cgpwgs.cwl rename examples/{analysis_config.local.json => cgpwgs/bam_bai.json} (100%) rename scripts/{ds-wrapper.pl => ds-cgpwgs.pl} (100%) diff --git a/CHANGES.md b/CHANGES.md index fddb175..9d98b9e 100644 --- a/CHANGES.md +++ b/CHANGES.md @@ -1,14 +1,24 @@ -### 1.1.2 +# CHANGES + +## 2.0.0 + +* Reorganisation to allow access of other tools or necessary bindings + +## 1.1.2 + * BRASS, random seed fixed to make noisy data have a reproducible result: * see [v6.0.4](https://github.com/cancerit/BRASS/releases/tag/v6.0.4) -### 1.1.1 +## 1.1.1 + * Stable results between replicate runs: * see [v6.0.3](https://github.com/cancerit/BRASS/releases/tag/v6.0.3) * took multiple executions of 29 datasets to find this edge-case. -### 1.1.0 +## 1.1.0 + BRASS updates + * Significantly reduces the run time of brass_group phase * see [v6.0.0](https://github.com/cancerit/BRASS/releases/tag/v6.0.0) * Requires new version of the CNV_SV reference archive @@ -16,6 +26,7 @@ BRASS updates * see [v6.0.2](https://github.com/cancerit/BRASS/releases/tag/v6.0.2) Other dependency updates + * Based on [dockstore-cgpwxs:2.1.1](https://github.com/cancerit/dockstore-cgpwxs/releases/tag/2.1.1) * Update caveman, pindel and vagrent to improve reproducibility. * Reduced I/O in caveman. @@ -24,6 +35,7 @@ Other dependency updates * [cgpBattenberg:v3.1.0](https://github.com/cancerit/cgpBattenberg/releases/tag/v3.1.0) Workflow updates: + * Reorganisation of some processes to reduce run-time: * Changes to alleleCount have vastly improved I/O pattern and reduced runtime for ascat and IMPUTE-allele counts (pre-Battenberg). @@ -31,32 +43,39 @@ ascat and IMPUTE-allele counts (pre-Battenberg). * Reduced alleleCount runtim allowed reorganisation of processes reducing runtime on example data from 23h to 19.5h (28cpu). * Added `cavereads` to example json specifying a higher value than default to reduce jobs required. -### 1.0.8 +## 1.0.8 + * Update [Brass to v5.4.1](https://github.com/cancerit/BRASS/releases/tag/v5.4.1) * Provide additional reference file rather than attempt to decode species/build due to the may ways a species can be defined. -### 1.0.7 +## 1.0.7 + * Update [BRASS to v5.4.0](https://github.com/cancerit/BRASS/releases/tag/v5.4.0) * Specifically fixes a vector merge bug which produces warnings (but no apparent change to results). -### 1.0.6 +## 1.0.6 + * Fixes #13 - Parsing of sample name from BAM headers fails is sample at end of line * Update dockstore-cgpwxs to [2.0.7](https://github.com/cancerit/dockstore-cgpwxs/releases/tag/2.0.7) - Fixes bug in pindel core. -### 1.0.5 +## 1.0.5 + * Improved handling of CPU oversubscription via PCAP-core update in base image * Update cgpCaVEManWrapper to expose extra options (specifically split size) -### 1.0.4 +## 1.0.4 + Fix an error in param capture test -### 1.0.3 +## 1.0.3 + * Export the `PCAP_THREADED_*` variables * Fixed #8, #9, #10 * Updated base image to pull in fix required for `PCAP_THREADED_LOADBACKOFF` -### 1.0.2 +## 1.0.2 + * Update BRASS to handle data that is _very_ quiet * Fix up the wrapper timings file so it is captured properly * Upgraded base image to [dockstore-cgpwxs:2.0.4](https://github.com/cancerit/dockstore-cgpwxs/releases/tag/2.0.4) @@ -64,10 +83,12 @@ Fix an error in param capture test * Remove ability to build `*.bas` files as not possible to have optional secondary files and we state expectation of [dockstore-cgpmap](https://github.com/cancerit/dockstore-cgpmap) as data source. * Moved some processing around to reduce cpu wastage. -### 1.0.1 +## 1.0.1 + * Update dependencies to reduce reliance on Capture::Tiny, apparent cause of some issues -### 1.0.0 +## 1.0.0 + * Test data in `examples/analysis_config.local.json` moved to a non-expiring location. * See [dockstore-cgpwxs:2.0.0](https://github.com/cancerit/dockstore-cgpwxs/releases/tag/2.0.0) * Significant shrinking of image. diff --git a/Dockerfile b/Dockerfile index 58ac783..109f474 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,41 +1,90 @@ -FROM quay.io/wtsicgp/dockstore-cgpwxs:2.1.1 +FROM quay.io/wtsicgp/dockstore-cgpwxs:3.0.0-rc2 as builder -MAINTAINER keiranmraine@gmail.com +USER root -LABEL uk.ac.sanger.cgp="Cancer Genome Project, Wellcome Trust Sanger Institute" \ - version="1.1.2" \ - description="The CGP WGS pipeline for dockstore.org" +RUB echo "deb http://cran.rstudio.com/bin/linux/ubuntu `lsb_release -cs`/" >> /etc/apt/sources.list +RUN gpg --keyserver keyserver.ubuntu.com --recv-key E084DAB9 +RUN gpg -a --export E084DAB9 | apt-key add - -USER root +RUN apt-get update +RUN apt-get apt-get install -qy --no-install-recommends\ + locales\ + g++\ + make\ + gcc\ + pkg-config\ + zlib1g-dev\ + libreadline6-dev\ + libcairo2-dev\ + gfortran\ + unzip\ + libboost-all-dev\ + libpstreams-dev\ + r-base\ + libblas-dev + +RUN locale-gen en_US.UTF-8 +RUN update-locale LANG=en_US.UTF-8 ENV OPT /opt/wtsi-cgp ENV PATH $OPT/bin:$PATH ENV PERL5LIB $OPT/lib/perl5 ENV LD_LIBRARY_PATH $OPT/lib +ENV LC_ALL en_US.UTF-8 +ENV LANG en_US.UTF-8 ENV R_LIBS $OPT/R-lib ENV R_LIBS_USER $R_LIBS -COPY build/apt-build.sh build/ -RUN bash build/apt-build.sh - COPY build/rlib-build.R build/ RUN mkdir -p $R_LIBS_USER && Rscript build/rlib-build.R $R_LIBS_USER -COPY build/perllib-build.sh build/ -RUN bash build/perllib-build.sh - COPY build/opt-build.sh build/ RUN bash build/opt-build.sh $OPT +FROM ubuntu:16.04 + +MAINTAINER keiranmraine@gmail.com + +LABEL uk.ac.sanger.cgp="Cancer Genome Project, Wellcome Trust Sanger Institute" \ + version="2.0.0-rc1" \ + description="The CGP WGS pipeline for dockstore.org" + +RUN apt-get -yq update +RUN apt-get install -yq --no-install-recommends\ + apt-transport-https\ + locales\ + curl\ + ca-certificates\ + libperlio-gzip-perl\ + bzip2\ + psmisc\ + time\ + zlib1g\ + liblzma5\ + libncurses5\ + libcairo2\ + gfortran\ + libboost-all\ + libpstreams\ + r-base\ + libblas + +RUN locale-gen en_US.UTF-8 +RUN update-locale LANG=en_US.UTF-8 + +ENV OPT /opt/wtsi-cgp +ENV PATH $OPT/bin:$PATH +ENV PERL5LIB $OPT/lib/perl5 +ENV LD_LIBRARY_PATH $OPT/lib +ENV LC_ALL en_US.UTF-8 +ENV LANG en_US.UTF-8 +ENV R_LIBS $OPT/R-lib +ENV R_LIBS_USER $R_LIBS + COPY scripts/analysisWGS.sh $OPT/bin/analysisWGS.sh COPY scripts/ds-wrapper.pl $OPT/bin/ds-wrapper.pl RUN chmod a+x $OPT/bin/analysisWGS.sh $OPT/bin/ds-wrapper.pl -### security upgrades and cleanup -RUN apt-get -yq update && \ - apt-get -yq install unattended-upgrades && \ - unattended-upgrades - USER ubuntu WORKDIR /home/ubuntu diff --git a/Dockstore.cwl b/Dockstore.cwl deleted file mode 100644 index 2640a01..0000000 --- a/Dockstore.cwl +++ /dev/null @@ -1,164 +0,0 @@ -#!/usr/bin/env cwl-runner - -class: CommandLineTool - -id: "cgpwgs" - -label: "CGP WGS analysis flow" - -cwlVersion: v1.0 - -doc: | - ![build_status](https://quay.io/repository/wtsicgp/dockstore-cgpwgs/status) - A Docker container for the CGP WGS analysis flow. See the [dockstore-cgpwgs](https://github.com/cancerit/dockstore-cgpwgs) - website for information relating to reference files. - - Example `json` run files are included in the repository. - -dct:creator: - "@id": "http://orcid.org/0000-0002-5634-1539" - foaf:name: Keiran M Raine - foaf:mbox: "keiranmraine@gmail.com" - -requirements: - - class: DockerRequirement - dockerPull: "quay.io/wtsicgp/dockstore-cgpwgs:1.1.2" - -hints: - - class: ResourceRequirement - coresMin: 1 # works but long, 24 recommended - ramMin: 32000 - outdirMin: 20000 - -inputs: - reference: - type: File - doc: "The core reference (fa, fai, dict) as tar.gz" - inputBinding: - prefix: -reference - position: 1 - separate: true - - annot: - type: File - doc: "The VAGrENT cache files" - inputBinding: - prefix: -annot - position: 2 - separate: true - - snv_indel: - type: File - doc: "Supporting files for SNV and INDEL analysis" - inputBinding: - prefix: -snv_indel - position: 3 - separate: true - - cnv_sv: - type: File - doc: "Supporting files for CNV and SV analysis" - inputBinding: - prefix: -cnv_sv - position: 4 - separate: true - - subcl: - type: File - doc: "Supporting files for allele counts used by Battenberg Subclonal CNV analysis" - inputBinding: - prefix: -subcl - position: 5 - separate: true - - tumour: - type: File - secondaryFiles: - - .bai - - .bas - doc: "Tumour BAM or CRAM file" - inputBinding: - prefix: -tumour - position: 6 - separate: true - - normal: - type: File - secondaryFiles: - - .bai - - .bas - doc: "Normal BAM or CRAM file" - inputBinding: - prefix: -normal - position: 7 - separate: true - - exclude: - type: string - doc: "Contigs to block during indel analysis" - inputBinding: - prefix: -exclude - position: 8 - separate: true - shellQuote: true - - species: - type: string? - doc: "Species to apply if not found in BAM headers" - default: '' - inputBinding: - prefix: -species - position: 9 - separate: true - shellQuote: true - - assembly: - type: string? - doc: "Assembly to apply if not found in BAM headers" - default: '' - inputBinding: - prefix: -assembly - position: 10 - separate: true - shellQuote: true - - skipbb: - type: boolean? - doc: "Skip Battenberg allele counts" - inputBinding: - prefix: -skipbb - position: 11 - separate: true - - cavereads: - type: int? - doc: "Number of reads in a split section for CaVEMan" - default: 350000 - inputBinding: - prefix: -cavereads - position: 12 - separate: true - -outputs: - run_params: - type: File - outputBinding: - glob: run.params - - result_archive: - type: File - outputBinding: - glob: WGS_*_vs_*.result.tar.gz - - # named like this so can be converted to a secondaryFile set once supported by dockstore cli - timings: - type: File - outputBinding: - glob: WGS_*_vs_*.timings.tar.gz - - global_time: - type: File - outputBinding: - glob: WGS_*_vs_*.time - -baseCommand: ["/opt/wtsi-cgp/bin/ds-wrapper.pl"] diff --git a/Dockstore.cwl b/Dockstore.cwl new file mode 120000 index 0000000..3d7d01f --- /dev/null +++ b/Dockstore.cwl @@ -0,0 +1 @@ +cwls/cgpwgs.cwl \ No newline at end of file diff --git a/build/apt-build.sh b/build/apt-build.sh deleted file mode 100644 index 998effb..0000000 --- a/build/apt-build.sh +++ /dev/null @@ -1,19 +0,0 @@ -#!/bin/bash - -set -eux - -UBUNTU_VER=`lsb_release -cs` - -echo "deb http://cran.rstudio.com/bin/linux/ubuntu $UBUNTU_VER/" >> /etc/apt/sources.list -gpg --keyserver keyserver.ubuntu.com --recv-key E084DAB9 -gpg -a --export E084DAB9 | apt-key add - - -apt-get update -apt-get install -qy --no-install-recommends libreadline6-dev -apt-get install -qy --no-install-recommends libcairo2-dev -apt-get install -qy --no-install-recommends gfortran -apt-get install -qy --no-install-recommends unzip -apt-get install -qy --no-install-recommends libboost-all-dev -apt-get install -qy --no-install-recommends libpstreams-dev -apt-get install -qy --no-install-recommends r-base -apt-get install -qy --no-install-recommends libblas-dev diff --git a/build/perllib-build.sh b/build/perllib-build.sh deleted file mode 100644 index 782620a..0000000 --- a/build/perllib-build.sh +++ /dev/null @@ -1,5 +0,0 @@ -#!/bin/bash - -set -eux - -echo "Doing nothing, here for perl libs that won't install through cpanminus" diff --git a/cwls/cgpwgs.cwl b/cwls/cgpwgs.cwl new file mode 100644 index 0000000..05b33cf --- /dev/null +++ b/cwls/cgpwgs.cwl @@ -0,0 +1,164 @@ +#!/usr/bin/env cwl-runner + +class: CommandLineTool + +id: "cgpwgs" + +label: "CGP WGS analysis flow" + +cwlVersion: v1.0 + +doc: | + ![build_status](https://quay.io/repository/wtsicgp/dockstore-cgpwgs/status) + A Docker container for the CGP WGS analysis flow. See the [dockstore-cgpwgs](https://github.com/cancerit/dockstore-cgpwgs) + website for information relating to reference files. + + Example `json` run files are included in the repository. + +dct:creator: + "@id": "http://orcid.org/0000-0002-5634-1539" + foaf:name: Keiran M Raine + foaf:mbox: "keiranmraine@gmail.com" + +requirements: + - class: DockerRequirement + dockerPull: "quay.io/wtsicgp/dockstore-cgpwgs:2.0.0-rc1" + +hints: + - class: ResourceRequirement + coresMin: 1 # works but long, 24 recommended + ramMin: 32000 + outdirMin: 20000 + +inputs: + reference: + type: File + doc: "The core reference (fa, fai, dict) as tar.gz" + inputBinding: + prefix: -reference + position: 1 + separate: true + + annot: + type: File + doc: "The VAGrENT cache files" + inputBinding: + prefix: -annot + position: 2 + separate: true + + snv_indel: + type: File + doc: "Supporting files for SNV and INDEL analysis" + inputBinding: + prefix: -snv_indel + position: 3 + separate: true + + cnv_sv: + type: File + doc: "Supporting files for CNV and SV analysis" + inputBinding: + prefix: -cnv_sv + position: 4 + separate: true + + subcl: + type: File + doc: "Supporting files for allele counts used by Battenberg Subclonal CNV analysis" + inputBinding: + prefix: -subcl + position: 5 + separate: true + + tumour: + type: File + secondaryFiles: + - .bai + - .bas + doc: "Tumour BAM or CRAM file" + inputBinding: + prefix: -tumour + position: 6 + separate: true + + normal: + type: File + secondaryFiles: + - .bai + - .bas + doc: "Normal BAM or CRAM file" + inputBinding: + prefix: -normal + position: 7 + separate: true + + exclude: + type: string + doc: "Contigs to block during indel analysis" + inputBinding: + prefix: -exclude + position: 8 + separate: true + shellQuote: true + + species: + type: string? + doc: "Species to apply if not found in BAM headers" + default: '' + inputBinding: + prefix: -species + position: 9 + separate: true + shellQuote: true + + assembly: + type: string? + doc: "Assembly to apply if not found in BAM headers" + default: '' + inputBinding: + prefix: -assembly + position: 10 + separate: true + shellQuote: true + + skipbb: + type: boolean? + doc: "Skip Battenberg allele counts" + inputBinding: + prefix: -skipbb + position: 11 + separate: true + + cavereads: + type: int? + doc: "Number of reads in a split section for CaVEMan" + default: 350000 + inputBinding: + prefix: -cavereads + position: 12 + separate: true + +outputs: + run_params: + type: File + outputBinding: + glob: run.params + + result_archive: + type: File + outputBinding: + glob: WGS_*_vs_*.result.tar.gz + + # named like this so can be converted to a secondaryFile set once supported by dockstore cli + timings: + type: File + outputBinding: + glob: WGS_*_vs_*.timings.tar.gz + + global_time: + type: File + outputBinding: + glob: WGS_*_vs_*.time + +baseCommand: ["/opt/wtsi-cgp/bin/ds-wrapper.pl"] diff --git a/examples/analysis_config.local.json b/examples/cgpwgs/bam_bai.json similarity index 100% rename from examples/analysis_config.local.json rename to examples/cgpwgs/bam_bai.json diff --git a/scripts/ds-wrapper.pl b/scripts/ds-cgpwgs.pl similarity index 100% rename from scripts/ds-wrapper.pl rename to scripts/ds-cgpwgs.pl From 09313337a1fb33b022e2044eeebc319fbf81778f Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 13 Feb 2018 09:25:16 +0000 Subject: [PATCH 05/57] More conversion and cleanup for docs and singularity --- .dockerignore | 6 +++ README.md | 106 ++++++++++++++++++++++++++++++----------- build/opt-build.sh | 2 +- scripts/analysisWGS.sh | 3 +- scripts/ds-cgpwgs.pl | 33 ++++++++++--- 5 files changed, 115 insertions(+), 35 deletions(-) create mode 100644 .dockerignore diff --git a/.dockerignore b/.dockerignore new file mode 100644 index 0000000..a637a6b --- /dev/null +++ b/.dockerignore @@ -0,0 +1,6 @@ +/cwls/ +/examples/ +/images/ +/git-hooks/ +/*.* +/LICENSE diff --git a/README.md b/README.md index f6e49db..9d1f5c6 100644 --- a/README.md +++ b/README.md @@ -1,45 +1,69 @@ -dockstore-cgpwgs -====== -`dockstore-cgpwgs` provides a complete multi threaded WGS analysis for SNV, INDEL, SV and Copynumber variation with associated annotation of VCF files. This has been packaged specifically for use with the [Dockstore.org](https://dockstore.org/) framework. +# dockstore-cgpwgs -[![Join the chat at https://gitter.im/dockstore-cgpwgs/general](https://badges.gitter.im/dockstore-cgpwgs/general.svg)](https://gitter.im/dockstore-cgpwgs/general?utm_source=badge&utm_medium=badge&utm_campaign=pr-badge&utm_content=badge) +`dockstore-cgpwgs` provides a complete multi threaded WGS analysis for SNV, INDEL, SV and Copynumber +variation with associated annotation of VCF files. This has been packaged specifically for use with +the [Dockstore.org](https://dockstore.org/) framework. -[![Docker Repository on Quay](https://quay.io/repository/wtsicgp/dockstore-cgpwgs/status "Docker Repository on Quay")](https://quay.io/repository/wtsicgp/dockstore-cgpwgs) +[![Gitter Badge][gitter-svg]][gitter-badge] -[![Build Status](https://travis-ci.org/cancerit/dockstore-cgpwgs.svg?branch=master)](https://travis-ci.org/cancerit/dockstore-cgpwgs) : master -[![Build Status](https://travis-ci.org/cancerit/dockstore-cgpwgs.svg?branch=develop)](https://travis-ci.org/cancerit/dockstore-cgpwgs) : develop +[![Quay Badge][quay-status]][quay-repo] + +| Master | Develop | +| --------------------------------------------- | ----------------------------------------------- | +| [![Master Badge][travis-master]][travis-base] | [![Develop Badge][travis-develop]][travis-base] | + +## Usage + +This is intended to be run using the Dockstore.org framework under docker but can be executed as a +normal docker container (or imported into singularity. + +You should see the usage for the `ds-cgpwgs.pl` script for all parameters (or the `cwl` definition). Required input files are 1. Tumour BAM file -2. Normal BAM file -3. Core reference archive (e.g. [core_ref_GRCh37d5.tar.gz](ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/)) -4. WXS reference archive (e.g. [SNV_INDEL_ref_GRCh37d5.tar.gz](ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/)) -5. WGS reference archive (e.g. [CNV_SV_ref_GRCh37d5_brass6+.tar.gz](ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/)) -6. VAGrENT (annotation) reference archive (e.g. [VAGrENT_ref_GRCh37d5_ensembl_75.tar.gz](ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/)) -7. Subclonal reference archive ([SUBCL_ref_GRCh37d5.tar.gz])(ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/)) +1. Normal BAM file +1. Core reference archive (e.g. [core_ref_GRCh37d5.tar.gz][ftp-ref]) +1. WXS reference archive (e.g. [SNV_INDEL_ref_GRCh37d5.tar.gz][ftp-ref]) +1. WGS reference archive (e.g. [CNV_SV_ref_GRCh37d5_brass6+.tar.gz][ftp-ref]) +1. VAGrENT (annotation) reference archive (e.g. [VAGrENT_ref_GRCh37d5_ensembl_75.tar.gz][ftp-ref]) +1. Subclonal reference archive ([SUBCL_ref_GRCh37d5.tar.gz][ftp-ref]) * Only needed if `skipbb` is `false` -Inputs 1&2 are expected to have been mapped using [dockstore-cgpmap](https://dockstore.org/containers/quay.io/wtsicgp/dockstore-cgpmap). +Inputs 1&2 are expected to have been mapped using [dockstore-cgpmap][dockstore-cgpmap] + +### Usable Cores + +When running outside of a docker container you can set the number of CPUs via: + +* `export CPU=N` +* `-cores|-c` option of `ds-cgpwgs.pl` + +If not set detects available cores on system. + +## Example data The data linked in the 'examples' area is from the cell line COLO-829. -Please check the Wiki then raise an issue if you require additional information on how to generate your own reference files. Much of this information is available on the individual algorithm wiki pages (or the subsequently linked protocols papers). +Please check the Wiki then raise an issue if you require additional information on how to generate +your own reference files. Much of this information is available on the individual algorithm wiki +pages (or the subsequently linked protocols papers). -* [BRASS](https://github.com/cancerit/BRASS/wiki) -* [cgpCaVEManWrapper](https://github.com/cancerit/cgpCaVEManWrapper/wiki) -* [cgpPindel](https://github.com/cancerit/cgpPindel/wiki) -* [ascatNgs](https://github.com/cancerit/ascatNgs/wiki) -* [VAGrENT](https://github.com/cancerit/VAGrENT/wiki) +* [BRASS][brass-wiki] +* [cgpCaVEManWrapper][caveman-wiki] +* [cgpPindel][cgppindel-wiki] +* [ascatNgs][ascatngs-wiki] +* [VAGrENT][vagrent-wiki] ## Diagram of internals -This diagram was generated based on v1.1.0, it does not describe any of the file provisioning handled by a Dockstore run. +This diagram was generated based on v1.1.0, it does not describe any of the file provisioning +handled by a Dockstore run. ![Internal flow of docker image](images/dockstore-cgpwgs.png) -Release process -=============== +## Release process + This project is maintained using HubFlow. 1. Make appropriate changes @@ -47,12 +71,12 @@ This project is maintained using HubFlow. 3. Push changes 4. Check state on Travis 5. Generate the release (add notes to GitHub) -6. Confirm that image has been built on [quay.io](https://quay.io/repository/wtsicgp/dockstore-cgpwgs?tab=builds) -7. Update the [dockstore](https://dockstore.org/containers/quay.io/wtsicgp/dockstore-cgpwgs) entry, see [their docs](https://dockstore.org/docs/getting-started-with-dockstore). +6. Confirm that image has been built on [quay.io][quay-builds] +7. Update the [dockstore][dockstore-cgpwgs] entry, see [their docs][dockstore-get-started]. -LICENCE -======= +## LICENCE +``` Copyright (c) 2017 Genome Research Ltd. Author: Cancer Genome Project @@ -81,3 +105,31 @@ reads ‘Copyright (c) 2005, 2007, 2008, 2009, 2011, 2012’ and a copyright statement that reads ‘Copyright (c) 2005-2012’ should be interpreted as being identical to a statement that reads ‘Copyright (c) 2005, 2006, 2007, 2008, 2009, 2010, 2011, 2012’." +``` + + +[ftp-ref]: ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human +[brass-wiki]: https://github.com/cancerit/BRASS/wiki +[cgppindel-wiki]: https://github.com/cancerit/cgpPindel/wiki +[caveman-wiki]: https://github.com/cancerit/cgpCaVEManWrapper/wiki +[ascatngs-wiki]: https://github.com/cancerit/ascatNgs/wiki +[vagrent-wiki]: https://github.com/cancerit/VAGrENT/wiki + + +[travis-base]: https://travis-ci.org/cancerit/dockstore-cgpwgs +[travis-master]: https://travis-ci.org/cancerit/dockstore-cgpwgs.svg?branch=master +[travis-develop]: https://travis-ci.org/cancerit/dockstore-cgpwgs.svg?branch=develop + + +[gitter-svg]: https://badges.gitter.im/dockstore-cgpwgs/general.svg +[gitter-badge]: https://gitter.im/dockstore-cgpwgs/general?utm_source=badge&utm_medium=badge&utm_campaign=pr-badge&utm_content=badge + + +[quay-status]: https://quay.io/repository/wtsicgp/dockstore-cgpwgs/status +[quay-repo]: https://quay.io/repository/wtsicgp/dockstore-cgpwgs +[quay-builds]: https://quay.io/repository/wtsicgp/dockstore-cgpwgs?tab=builds + + +[dockstore-cgpwgs]: https://dockstore.org/containers/quay.io/wtsicgp/dockstore-cgpwgs +[dockstore-cgpmap]: https://dockstore.org/containers/quay.io/wtsicgp/dockstore-cgpmap +[dockstore-get-started]: https://dockstore.org/docs/getting-started-with-dockstore diff --git a/build/opt-build.sh b/build/opt-build.sh index a29c425..fc2b35e 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -9,7 +9,7 @@ fi set -u ### alleleCount -VER_ALLELECOUNT="v3.3.0" +VER_ALLELECOUNT="feature/overlapping_reads" ### cgpNgsQc VER_CGPNGSQC="v1.4.0" diff --git a/scripts/analysisWGS.sh b/scripts/analysisWGS.sh index efc0cd9..226a7f9 100755 --- a/scripts/analysisWGS.sh +++ b/scripts/analysisWGS.sh @@ -149,6 +149,7 @@ do_parallel[CaVEMan_setup]="caveman.pl \ -f $REF_BASE/caveman/flagging/flag.to.vcf.convert.ini \ -e $CAVESPLIT \ -o $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/caveman \ + -x $CONTIG_EXCLUDE \ -p setup" echo -e "\t[Parallel block 1] BB splitlocifiles added..." @@ -317,7 +318,7 @@ do_parallel[cgpPindel]="nice -n 10 pindel.pl \ -st $PROTOCOL \ -as $ASSEMBLY \ -sp '$SPECIES' \ - -e $PINDEL_EXCLUDE \ + -e $CONTIG_EXCLUDE \ -b $REF_BASE/pindel/HiDepth.bed.gz \ -c $CPU \ -sf $REF_BASE/pindel/softRules.lst" diff --git a/scripts/ds-cgpwgs.pl b/scripts/ds-cgpwgs.pl index 6979be6..7b92338 100644 --- a/scripts/ds-cgpwgs.pl +++ b/scripts/ds-cgpwgs.pl @@ -12,7 +12,11 @@ pod2usage(-verbose => 1, -exitval => 1) if(@ARGV == 0); # set defaults -my %opts = (); +my %opts = ('c' => undef, + 'o' => $ENV{HOME}, + 'sp' => undef, + 'as' => undef, + ); GetOptions( 'h|help' => \$opts{'h'}, 'm|man' => \$opts{'m'}, @@ -24,10 +28,12 @@ 't|tumour=s' => \$opts{'t'}, 'n|normal=s' => \$opts{'n'}, 'e|exclude=s' => \$opts{'e'}, - 'sp|species=s' => \$opts{'sp'}, - 'as|assembly=s' => \$opts{'as'}, + 'sp|species:s' => \$opts{'sp'}, + 'as|assembly:s' => \$opts{'as'}, 'sb|skipbb' => \$opts{'sb'}, - 'cr|cavereads=i' => \$opts{'cr'} + 'cr|cavereads=i' => \$opts{'cr'}, + 'c|cores:i' => \$opts{'c'}, + 'o|outdir:s' => \$opts{'o'}, ) or pod2usage(2); pod2usage(-verbose => 1, -exitval => 0) if(defined $opts{'h'}); @@ -102,11 +108,13 @@ printf $FH "REF_BASE='%s'\n", $ref_area; printf $FH "BAM_MT='%s'\n", $opts{'t'}; printf $FH "BAM_WT='%s'\n", $opts{'n'}; -printf $FH "PINDEL_EXCLUDE='%s'\n", $opts{'e'}; +printf $FH "CONTIG_EXCLUDE='%s'\n", $opts{'e'}; printf $FH "SPECIES='%s'\n", $opts{'sp'}; printf $FH "ASSEMBLY='%s'\n", $opts{'as'}; printf $FH "CAVESPLIT='%s'\n", $opts{'cr'}; printf $FH "SNVFLAG='%s'\n", $ini; +printf $FH "CPU=%d\n", $opts{'c'} if(defined $opts{'c'}); +printf $FH "CLEAN_REF=1\n" if($ref_unpack); # Options to disable algorithms print $FH "SKIPBB=1\n" if(exists $opts{'sb'}); close $FH; @@ -217,13 +225,15 @@ =head1 SYNOPSIS -subcl -sc Path to SUBCL*.tar.gz -tumour -t Tumour [CR|B]AM file -normal -n Normal [CR|B]AM file - -exclude -e Exclude these contigs from pindel analysis + -exclude -e Exclude these contigs from SNV/Indel analysis e.g. NC_007605,hs37d5,GL% Optional parameters -species -sp Species name (may require quoting) -assembly -a Reference assembly -skipbb -sb Skip Battenberg allele counts + -outdir -o Set the output folder [$HOME] + -cores -c Set the number of cpu/cores available [default all]. Other: -help -h Brief help message. @@ -283,6 +293,17 @@ =head1 OPTION DETAILS Disables the Battenberg allele count generation +=item B<-outdir> + +Set the output directory. Defaults to $HOME. + +NOTE: Should B be set when working with dockstore wrapper. + +=item B<-cores> + +Sets the number of cores to be used during processing. Default to use all at appropriate +points in analysis. + =back =cut From f32aac3942b3792795d0c2597ed64070a8066a7a Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 13 Feb 2018 10:02:10 +0000 Subject: [PATCH 06/57] Some cleanup and prep for support of cram, may allow cram by turning off verifyBamId --- scripts/analysisWGS.sh | 75 ++++++++++++++++++++---------------------- 1 file changed, 36 insertions(+), 39 deletions(-) diff --git a/scripts/analysisWGS.sh b/scripts/analysisWGS.sh index 226a7f9..2afb8a3 100755 --- a/scripts/analysisWGS.sh +++ b/scripts/analysisWGS.sh @@ -77,30 +77,9 @@ fi echo -e "\tBAM_MT : $BAM_MT" echo -e "\tBAM_WT : $BAM_WT" -if [ ${#PRE_EXEC[@]} -eq 0 ]; then - PRE_EXEC='echo No PRE_EXEC defined' -fi - -if [ ${#POST_EXEC[@]} -eq 0 ]; then - POST_EXEC='echo No POST_EXEC defined' -fi - set -u mkdir -p $OUTPUT_DIR -# run any pre-exec step before attempting to access BAMs -# logically the pre-exec could be pulling them -if [ ! -f $OUTPUT_DIR/pre-exec.done ]; then - echo -e "\nRun PRE_EXEC: `date`" - - for i in "${PRE_EXEC[@]}"; do - set -x - $i - { set +x; } 2> /dev/null - done - touch $OUTPUT_DIR/pre-exec.done -fi - ## get sample names from BAM headers NAME_MT=`samtools view -H $BAM_MT | perl -ne 'chomp; if($_ =~ m/^\@RG/) {($sm) = $_ =~m/\tSM:([^\t]+)/; print "$sm\n";}' | uniq` NAME_WT=`samtools view -H $BAM_WT | perl -ne 'chomp; if($_ =~ m/^\@RG/) {($sm) = $_ =~m/\tSM:([^\t]+)/; print "$sm\n";}' | uniq` @@ -108,26 +87,47 @@ NAME_WT=`samtools view -H $BAM_WT | perl -ne 'chomp; if($_ =~ m/^\@RG/) {($sm) = echo -e "\tNAME_MT : $NAME_MT" echo -e "\tNAME_WT : $NAME_WT" -BAM_MT_TMP=$TMP/$NAME_MT.bam -BAM_WT_TMP=$TMP/$NAME_WT.bam +# capture index extension type (assuming same from both) +ALN_EXTN='bam' +IDX_EXTN='' +if [[ "$IDX_MT" == *.bam.bai ]]; then + IDX_EXTN='bam.bai' +elif [[ "$IDX_MT" == *.bam.csi ]]; then + IDX_EXTN='bam.csi' +elif [[ "$IDX_MT" == *.cram.crai ]]; then + IDX_EXTN='cram.crai' + ALN_EXTN='cram' +else + echo "Alignment is not BAM or CRAM file: $" >&2 + exit 1 +fi + +BAM_MT_TMP=$TMP/$NAME_MT.$ALN_EXTN +IDX_MT_TMP=$TMP/$NAME_MT.$IDX_EXTN +BAM_WT_TMP=$TMP/$NAME_WT.$ALN_EXTN +IDX_WT_TMP=$TMP/$NAME_WT.$IDX_EXTN + +## BAS files are not generated in this flow due to siginifcant run time overhead. ln -fs $BAM_MT $BAM_MT_TMP -ln -fs $BAM_WT $BAM_WT_TMP -ln -fs $BAM_MT.bai $BAM_MT_TMP.bai -ln -fs $BAM_WT.bai $BAM_WT_TMP.bai ln -fs $BAM_MT.bas $BAM_MT_TMP.bas +ln -fs $IDX_MT $IDX_MT_TMP +ln -fs $BAM_WT $BAM_WT_TMP ln -fs $BAM_WT.bas $BAM_WT_TMP.bas +ln -fs $IDX_WT $IDX_WT_TMP ## Make fake copynumber so we can run early steps of caveman perl -alne 'print join(qq{\t},$F[0],0,$F[1],2);' < $REF_BASE/genome.fa.fai | tee $TMP/norm.cn.bed > $TMP/tum.cn.bed echo "Setting up Parallel block 1" -## prime the cache -USER_CACHE=$OUTPUT_DIR/ref_cache -export REF_CACHE=$USER_CACHE/%2s/%2s/%s -export REF_PATH=$REF_CACHE:http://www.ebi.ac.uk/ena/cram/md5/%s -do_parallel[cache_POP]="seq_cache_populate.pl -root $USER_CACHE $REF_BASE/genome.fa" +if [ "$ALN_EXTN" == "cram" ]; then + ## prime the cache + USER_CACHE=$OUTPUT_DIR/ref_cache + export REF_CACHE=$USER_CACHE/%2s/%2s/%s + export REF_PATH=$REF_CACHE:http://www.ebi.ac.uk/ena/cram/md5/%s + do_parallel[cache_POP]="seq_cache_populate.pl -root $USER_CACHE $REF_BASE/genome.fa" +fi echo -e "\t[Parallel block 1] CaVEMan setup added..." do_parallel[CaVEMan_setup]="caveman.pl \ @@ -432,6 +432,11 @@ rm -f $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/battenberg/tmpBattenberg/ mv $OUTPUT_DIR/timings/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.time.verify_WT $OUTPUT_DIR/timings/${PROTOCOL}_${NAME_WT}.time.verify_WT mv $OUTPUT_DIR/timings/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.time.verify_MT $OUTPUT_DIR/timings/${PROTOCOL}_${NAME_MT}.time.verify_MT +# cleanup reference area, see ds-cgpwxs.pl +if [ ! -z ${CLEAN_REF+x} ]; then + rm -rf $REF_BASE +fi + # cleanup ref cache rm -rf $USER_CACHE @@ -441,12 +446,4 @@ tar -C $OUTPUT_DIR -zcf ${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.timings.tar.gz timi tar -C $OUTPUT_DIR -zcf ${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.result.tar.gz ${PROTOCOL}_${NAME_MT}_vs_${NAME_WT} ${PROTOCOL}_${NAME_MT} ${PROTOCOL}_${NAME_WT} cp $PARAM_FILE ${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.run.params -# run any post-exec step -echo -e "\nRun POST_EXEC: `date`" -for i in "${POST_EXEC[@]}"; do - set -x - $i - set +x -done - echo -e "\nWorkflow end: `date`" From 216184630404bf014a91163afac512e389583e0f Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 13 Feb 2018 14:27:36 +0000 Subject: [PATCH 07/57] Use packaged exonerate and upgrade brass --- Dockerfile | 16 ++++++++++++---- build/opt-build.sh | 30 +++++++++++++++--------------- 2 files changed, 27 insertions(+), 19 deletions(-) diff --git a/Dockerfile b/Dockerfile index 109f474..30756f9 100644 --- a/Dockerfile +++ b/Dockerfile @@ -2,13 +2,18 @@ FROM quay.io/wtsicgp/dockstore-cgpwxs:3.0.0-rc2 as builder USER root -RUB echo "deb http://cran.rstudio.com/bin/linux/ubuntu `lsb_release -cs`/" >> /etc/apt/sources.list +RUN apt-get update +RUN apt-get install -qy --no-install-recommends lsb-release + +RUN echo "deb http://cran.rstudio.com/bin/linux/ubuntu `lsb_release -cs`/" >> /etc/apt/sources.list RUN gpg --keyserver keyserver.ubuntu.com --recv-key E084DAB9 RUN gpg -a --export E084DAB9 | apt-key add - RUN apt-get update -RUN apt-get apt-get install -qy --no-install-recommends\ +RUN apt-get install -qy --no-install-recommends\ locales\ + libcurl4-openssl-dev\ + libssl-dev\ g++\ make\ gcc\ @@ -21,7 +26,9 @@ RUN apt-get apt-get install -qy --no-install-recommends\ libboost-all-dev\ libpstreams-dev\ r-base\ - libblas-dev + r-base-dev\ + libblas-dev\ + libbz2-dev RUN locale-gen en_US.UTF-8 RUN update-locale LANG=en_US.UTF-8 @@ -67,7 +74,8 @@ RUN apt-get install -yq --no-install-recommends\ libboost-all\ libpstreams\ r-base\ - libblas + libblas\ + exonerate RUN locale-gen en_US.UTF-8 RUN update-locale LANG=en_US.UTF-8 diff --git a/build/opt-build.sh b/build/opt-build.sh index fc2b35e..578c7ed 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -23,7 +23,7 @@ SRC_ASCAT="https://raw.githubusercontent.com/Crick-CancerGenomics/ascat/6d40e69a VER_GRASS="v2.1.0" ### BRASS -VER_BRASS="v6.0.4" +VER_BRASS="v6.0.6" SOURCE_BLAT="http://users.soe.ucsc.edu/~kent/src/blatSrc35.zip" SRC_FASTA36="https://github.com/wrpearson/fasta36/archive/v36.3.8d_13Apr16.tar.gz" @@ -194,20 +194,20 @@ if [ ! -e $SETUP_DIR/BRASS.success ]; then touch $SETUP_DIR/velvet.success fi - if [ ! -e $SETUP_DIR/exonerate.success ]; then - cd $SETUP_DIR/distro/distros - tar zxf exonerate-2.2.0.tar.gz - cd exonerate-2.2.0 - cp ../patches/exonerate_pthread-asneeded.diff . - patch -p1 < exonerate_pthread-asneeded.diff - ./configure --prefix=$INST_PATH - make # don't do multi-threaded make - make check - make install - cd $SETUP_DIR/distro - rm -rf distros/exonerate-2.2.0 - touch $SETUP_DIR/exonerate.success - fi + # if [ ! -e $SETUP_DIR/exonerate.success ]; then + # cd $SETUP_DIR/distro/distros + # tar zxf exonerate-2.2.0.tar.gz + # cd exonerate-2.2.0 + # cp ../patches/exonerate_pthread-asneeded.diff . + # patch -p1 < exonerate_pthread-asneeded.diff + # ./configure --prefix=$INST_PATH + # make # don't do multi-threaded make + # make check + # make install + # cd $SETUP_DIR/distro + # rm -rf distros/exonerate-2.2.0 + # touch $SETUP_DIR/exonerate.success + # fi cpanm --no-interactive --notest --mirror http://cpan.metacpan.org -l $INST_PATH Graph cpanm --no-interactive --notest --mirror http://cpan.metacpan.org -l $INST_PATH Bio::Tools::Run::WrapperBase From 00f0fb3473cb03af7cb14ef998d51942c5c88cdf Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 14 Feb 2018 04:48:07 +0000 Subject: [PATCH 08/57] Detail changes --- CHANGES.md | 6 ++++++ 1 file changed, 6 insertions(+) diff --git a/CHANGES.md b/CHANGES.md index 9d98b9e..a17d011 100644 --- a/CHANGES.md +++ b/CHANGES.md @@ -3,6 +3,12 @@ ## 2.0.0 * Reorganisation to allow access of other tools or necessary bindings +* See dockstore-cgpmap 3.0.0 (primarily adds mismatchQc) +* See dockstore-cgpwxs 3.0.0 (fragment based analysis) + * You will need to update the reference pack to include the new flagging rules, see example `json` + files +* Using build stages to shrink images. +* remove legacy PRE/POST-EXEC from cgpbox days ## 1.1.2 From c7959371bebb285a8c02a6150e9c8a9071f0ae23 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 14 Feb 2018 04:48:38 +0000 Subject: [PATCH 09/57] Move to shared gitter channel for all dockstore-cgp* repos --- README.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/README.md b/README.md index 9d1f5c6..6e90d43 100644 --- a/README.md +++ b/README.md @@ -121,8 +121,8 @@ identical to a statement that reads ‘Copyright (c) 2005, 2006, 2007, 2008, [travis-develop]: https://travis-ci.org/cancerit/dockstore-cgpwgs.svg?branch=develop -[gitter-svg]: https://badges.gitter.im/dockstore-cgpwgs/general.svg -[gitter-badge]: https://gitter.im/dockstore-cgpwgs/general?utm_source=badge&utm_medium=badge&utm_campaign=pr-badge&utm_content=badge +[gitter-svg]: https://badges.gitter.im/dockstore-cgp/Lobby.svg +[gitter-badge]: https://gitter.im/dockstore-cgp/Lobby?utm_source=badge&utm_medium=badge&utm_campaign=pr-badge&utm_content=badge [quay-status]: https://quay.io/repository/wtsicgp/dockstore-cgpwgs/status From c31ff871f0ca3b26ba8483867ede050f050899db Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 14 Feb 2018 04:49:28 +0000 Subject: [PATCH 10/57] Improve tool doc and point to renamed script --- cwls/cgpwgs.cwl | 18 +++++++++++++----- 1 file changed, 13 insertions(+), 5 deletions(-) diff --git a/cwls/cgpwgs.cwl b/cwls/cgpwgs.cwl index 05b33cf..7c1bdd7 100644 --- a/cwls/cgpwgs.cwl +++ b/cwls/cgpwgs.cwl @@ -9,11 +9,19 @@ label: "CGP WGS analysis flow" cwlVersion: v1.0 doc: | - ![build_status](https://quay.io/repository/wtsicgp/dockstore-cgpwgs/status) - A Docker container for the CGP WGS analysis flow. See the [dockstore-cgpwgs](https://github.com/cancerit/dockstore-cgpwgs) - website for information relating to reference files. + ![build_status](https://quay.io/repository/wtsicgp/dockstore-cgpwgs/status) + A Docker container for the CGP WXS analysis flow. See the [dockstore-cgpwgs](https://github.com/cancerit/dockstore-cgpwgs) + website for more information. - Example `json` run files are included in the repository. + Please read the relevant [changes](https://github.com/cancerit/dockstore-cgpwgs/blob/master/CHANGES.md) + when upgrading. + + Parameters for a CWL definition are generally described in a json file, but parameters can be provided on the command line. + + To see the parameters descriptions please run: cwltool --tool-help path_to.cwl + + WARNING: The usual setting for 'exclude' is 'NC_007605,hs37d5,GL%' (human GRCh37/NCBI37). Examples + are configured to run as quickly as possible. dct:creator: "@id": "http://orcid.org/0000-0002-5634-1539" @@ -161,4 +169,4 @@ outputs: outputBinding: glob: WGS_*_vs_*.time -baseCommand: ["/opt/wtsi-cgp/bin/ds-wrapper.pl"] +baseCommand: ["/opt/wtsi-cgp/bin/ds-cgpwgs.pl"] From c2da6a81170f384f28a5604b2d250020504a2880 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 14 Feb 2018 04:50:09 +0000 Subject: [PATCH 11/57] switch to smaller example set --- examples/cgpwgs/bam_bai.json | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/examples/cgpwgs/bam_bai.json b/examples/cgpwgs/bam_bai.json index 53cbab7..96717aa 100644 --- a/examples/cgpwgs/bam_bai.json +++ b/examples/cgpwgs/bam_bai.json @@ -8,7 +8,7 @@ "class": "File" }, "snv_indel": { - "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/SNV_INDEL_ref_GRCh37d5.tar.gz", + "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/SNV_INDEL_ref_GRCh37d5-fragment.tar.gz", "class": "File" }, "cnv_sv": { @@ -20,14 +20,14 @@ "class": "File" }, "tumour": { - "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/COLO-829.sample.dupmarked.bam", + "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/12x/COLO-829.bam", "class": "File" }, "normal": { - "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/COLO-829-BL.sample.dupmarked.bam", + "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/12x/COLO-829-BL.bam", "class": "File" }, - "exclude": "NC_007605,hs37d5,GL%", + "exclude": "1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,22,X,Y,MT,NC_007605,hs37d5,GL%", "species": "human", "assembly": "GRCh37d5", "cavereads": 800000, From 6fbe75fa077ba95870d89e7bc2243304610d05e2 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 14 Feb 2018 04:50:48 +0000 Subject: [PATCH 12/57] Cleanup and correction of renamed script references --- Dockerfile | 16 ++++++++-------- 1 file changed, 8 insertions(+), 8 deletions(-) diff --git a/Dockerfile b/Dockerfile index 30756f9..312e8ab 100644 --- a/Dockerfile +++ b/Dockerfile @@ -52,9 +52,9 @@ FROM ubuntu:16.04 MAINTAINER keiranmraine@gmail.com -LABEL uk.ac.sanger.cgp="Cancer Genome Project, Wellcome Trust Sanger Institute" \ - version="2.0.0-rc1" \ - description="The CGP WGS pipeline for dockstore.org" +LABEL vendor="Cancer Genome Project, Wellcome Trust Sanger Institute" +LABEL uk.ac.sanger.cgp.description="CGP WGS pipeline for dockstore.org" +LABEL uk.ac.sanger.cgp.version="2.0.0-rc1" RUN apt-get -yq update RUN apt-get install -yq --no-install-recommends\ @@ -71,10 +71,7 @@ RUN apt-get install -yq --no-install-recommends\ libncurses5\ libcairo2\ gfortran\ - libboost-all\ - libpstreams\ r-base\ - libblas\ exonerate RUN locale-gen en_US.UTF-8 @@ -89,9 +86,12 @@ ENV LANG en_US.UTF-8 ENV R_LIBS $OPT/R-lib ENV R_LIBS_USER $R_LIBS +RUN mkdir -p $OPT +COPY --from=builder $OPT $OPT + COPY scripts/analysisWGS.sh $OPT/bin/analysisWGS.sh -COPY scripts/ds-wrapper.pl $OPT/bin/ds-wrapper.pl -RUN chmod a+x $OPT/bin/analysisWGS.sh $OPT/bin/ds-wrapper.pl +COPY scripts/ds-cgpwgs.pl $OPT/bin/ds-cgpwgs.pl +RUN chmod a+x $OPT/bin/analysisWGS.sh $OPT/bin/ds-cgpwgs.pl USER ubuntu WORKDIR /home/ubuntu From 961e6614d84c61473a431046e25b90b2f3174a66 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 14 Feb 2018 04:51:50 +0000 Subject: [PATCH 13/57] Handle remainder of switch to output location option --- scripts/analysisWGS.sh | 8 ++++---- scripts/ds-cgpwgs.pl | 35 ++++++++++++++++++++++------------- 2 files changed, 26 insertions(+), 17 deletions(-) diff --git a/scripts/analysisWGS.sh b/scripts/analysisWGS.sh index 2afb8a3..5fac2dc 100755 --- a/scripts/analysisWGS.sh +++ b/scripts/analysisWGS.sh @@ -49,12 +49,12 @@ set -e echo -e "\nStart workflow: `date`\n" -declare -a PRE_EXEC -declare -a POST_EXEC - -if [ -z ${PARAM_FILE+x} ] ; then +if [[ $# -eq 1 ]] ; then + PARAM_FILE=$1 +elif [ -z ${PARAM_FILE+x} ] ; then PARAM_FILE=$HOME/run.params fi + echo "Loading user options from: $PARAM_FILE" if [ ! -f $PARAM_FILE ]; then echo -e "\tERROR: file indicated by PARAM_FILE not found: $PARAM_FILE" 1>&2 diff --git a/scripts/ds-cgpwgs.pl b/scripts/ds-cgpwgs.pl index 7b92338..b7fe940 100644 --- a/scripts/ds-cgpwgs.pl +++ b/scripts/ds-cgpwgs.pl @@ -79,14 +79,24 @@ printf "Options loaded: \n%s\n",Dumper(\%opts); -## unpack the reference area: -my $ref_area = $ENV{HOME}.'/reference_files'; - -ref_unpack($ref_area, $opts{'r'}); -ref_unpack($ref_area, $opts{'a'}); -ref_unpack($ref_area, $opts{'si'}); -ref_unpack($ref_area, $opts{'cs'}); -ref_unpack($ref_area, $opts{'sc'}) if(! exists $opts{'sb'}); +# figure out if ref already unpacked: +my $ref_area = $opts{'o'}.'/reference_files'; +my $ref_unpack = 1; +if($opts{'r'} eq $opts{'a'} + && $opts{'r'} eq $opts{'si'} + && $opts{'r'} eq $opts{'cs'} + && (exists $opts{'sb'} || $opts{'r'} eq $opts{'sc'}) + && -d $opts{'r'}) { + $ref_area = $opts{'r'}; + $ref_unpack = 0; +} +else { + ref_unpack($ref_area, $opts{'r'}); + ref_unpack($ref_area, $opts{'a'}); + ref_unpack($ref_area, $opts{'si'}); + ref_unpack($ref_area, $opts{'cs'}); + ref_unpack($ref_area, $opts{'sc'}) if(! exists $opts{'sb'}); +} ## now complete the caveman flaging file correctly my $ini = add_species_flag_ini($opts{'sp'}, "$ref_area/caveman/flag.vcf.config.WGS.ini"); @@ -94,7 +104,7 @@ ## CWL changes $HOME so need to copy in relevant config for R copy('/home/ubuntu/.Rprofile', $ENV{HOME}.'/.Rprofile'); -my $run_file = $ENV{HOME}.'/run.params'; +my $run_file = $opts{'o'}.'/run.params'; open my $FH,'>',$run_file or die "Failed to write to $run_file: $!"; # Force explicit checking of file flush print $FH "export PCAP_THREADED_NO_SCRIPT=1\n"; @@ -104,7 +114,7 @@ # hard-coded printf $FH "PROTOCOL=WGS\n"; # required options -printf $FH "OUTPUT_DIR='%s'\n", $ENV{HOME}; +printf $FH "OUTPUT_DIR='%s'\n", $opts{'o'}}; printf $FH "REF_BASE='%s'\n", $ref_area; printf $FH "BAM_MT='%s'\n", $opts{'t'}; printf $FH "BAM_WT='%s'\n", $opts{'n'}; @@ -124,14 +134,13 @@ print $R_FH qq{options(bitmapType='cairo')\n}; close $R_FH; -my $cmd = sprintf '/usr/bin/time -o %s/WGS_%s_vs_%s.time -v /opt/wtsi-cgp/bin/analysisWGS.sh', $ENV{HOME}, $opts{'mt_sm'}, $opts{'wt_sm'}; -exec($cmd); # I will never return to the perl code +exec('analysisWGS.sh', $run_file); # I will never return to the perl code sub add_species_flag_ini { my ($species, $ini_in) = @_; $species =~ s/ /_/g; $species = uc $species; - my $ini_out = $ENV{HOME}.'/flag.vcf.config.WXS.ini'; + my $ini_out = $opts{'o'}.'/flag.vcf.config.WXS.ini'; open my $IN, '<', $ini_in; open my $OUT,'>',$ini_out; while(my $line = <$IN>) { From b59cf5ed5249e4d85205de696e7686adb72142ab Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 14 Feb 2018 12:13:45 +0000 Subject: [PATCH 14/57] Findings of first test-run --- cwls/cgpwgs.cwl | 28 ++++++++++++++++++++++------ examples/cgpwgs/bam_bai.json | 9 +++++++++ scripts/analysisWGS.sh | 10 +++++++++- scripts/ds-cgpwgs.pl | 6 +++++- 4 files changed, 45 insertions(+), 8 deletions(-) diff --git a/cwls/cgpwgs.cwl b/cwls/cgpwgs.cwl index 7c1bdd7..4b4d1ba 100644 --- a/cwls/cgpwgs.cwl +++ b/cwls/cgpwgs.cwl @@ -90,6 +90,14 @@ inputs: position: 6 separate: true + tumourIdx: + type: File + doc: "Tumour alignment file index [bai|csi|crai]" + inputBinding: + prefix: -tidx + position: 7 + separate: true + normal: type: File secondaryFiles: @@ -98,7 +106,15 @@ inputs: doc: "Normal BAM or CRAM file" inputBinding: prefix: -normal - position: 7 + position: 8 + separate: true + + normalIdx: + type: File + doc: "Normal alignment file index" + inputBinding: + prefix: -nidx + position: 9 separate: true exclude: @@ -106,7 +122,7 @@ inputs: doc: "Contigs to block during indel analysis" inputBinding: prefix: -exclude - position: 8 + position: 10 separate: true shellQuote: true @@ -116,7 +132,7 @@ inputs: default: '' inputBinding: prefix: -species - position: 9 + position: 11 separate: true shellQuote: true @@ -126,7 +142,7 @@ inputs: default: '' inputBinding: prefix: -assembly - position: 10 + position: 12 separate: true shellQuote: true @@ -135,7 +151,7 @@ inputs: doc: "Skip Battenberg allele counts" inputBinding: prefix: -skipbb - position: 11 + position: 13 separate: true cavereads: @@ -144,7 +160,7 @@ inputs: default: 350000 inputBinding: prefix: -cavereads - position: 12 + position: 14 separate: true outputs: diff --git a/examples/cgpwgs/bam_bai.json b/examples/cgpwgs/bam_bai.json index 96717aa..d6db44b 100644 --- a/examples/cgpwgs/bam_bai.json +++ b/examples/cgpwgs/bam_bai.json @@ -23,14 +23,23 @@ "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/12x/COLO-829.bam", "class": "File" }, + "tumourIdx": { + "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/12x/COLO-829.bam.bai", + "class": "File" + }, "normal": { "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/12x/COLO-829-BL.bam", "class": "File" }, + "normalIdx": { + "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/12x/COLO-829-BL.bam.bai", + "class": "File" + }, "exclude": "1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,22,X,Y,MT,NC_007605,hs37d5,GL%", "species": "human", "assembly": "GRCh37d5", "cavereads": 800000, + "skipbb": true, "result_archive": { "path": "/tmp/result_WGS.tar.gz", "class": "File" diff --git a/scripts/analysisWGS.sh b/scripts/analysisWGS.sh index 5fac2dc..a68b2fb 100755 --- a/scripts/analysisWGS.sh +++ b/scripts/analysisWGS.sh @@ -348,6 +348,14 @@ do_parallel[CaVEMan]="caveman.pl \ echo "Starting Parallel block 4: `date`" run_parallel do_parallel +GERMLINE_BED=$OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/pindel/${NAME_MT}_vs_${NAME_WT}.germline.bed +if [ -f $GERMLINE_BED ]; then + # need to sort and index pindel germline + sort -k1,1 -k2,2n -k3,3n $GERMLINE_BED | bgzip -c > $GERMLINE_BED.gz + tabix -p bed $GERMLINE_BED.gz + rm -f $GERMLINE_BED +fi + # unset and redeclare the parallel array ready for next block unset do_parallel declare -A do_parallel @@ -386,7 +394,7 @@ do_parallel[cgpFlagCaVEMan]="cgpFlagCaVEMan.pl \ -m $BAM_MT_TMP \ -n $BAM_WT_TMP \ -b $REF_BASE/caveman/flagging \ - -g $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/pindel/${NAME_MT}_vs_${NAME_WT}.germline.bed \ + -g $GERMLINE_BED.gz \ -umv $REF_BASE/caveman \ -ab $REF_BASE/vagrent \ -ref $REF_BASE/genome.fa.fai \ diff --git a/scripts/ds-cgpwgs.pl b/scripts/ds-cgpwgs.pl index b7fe940..7cafbd0 100644 --- a/scripts/ds-cgpwgs.pl +++ b/scripts/ds-cgpwgs.pl @@ -26,7 +26,9 @@ 'cs|cnv_sv=s' => \$opts{'cs'}, 'sc|subcl=s' => \$opts{'sc'}, 't|tumour=s' => \$opts{'t'}, + 'tidx=s' => \$opts{'tidx'}, 'n|normal=s' => \$opts{'n'}, + 'nidx=s' => \$opts{'nidx'}, 'e|exclude=s' => \$opts{'e'}, 'sp|species:s' => \$opts{'sp'}, 'as|assembly:s' => \$opts{'as'}, @@ -114,10 +116,12 @@ # hard-coded printf $FH "PROTOCOL=WGS\n"; # required options -printf $FH "OUTPUT_DIR='%s'\n", $opts{'o'}}; +printf $FH "OUTPUT_DIR='%s'\n", $opts{'o'}; printf $FH "REF_BASE='%s'\n", $ref_area; printf $FH "BAM_MT='%s'\n", $opts{'t'}; +printf $FH "IDX_MT='%s'\n", $opts{'tidx'}; printf $FH "BAM_WT='%s'\n", $opts{'n'}; +printf $FH "IDX_WT='%s'\n", $opts{'nidx'}; printf $FH "CONTIG_EXCLUDE='%s'\n", $opts{'e'}; printf $FH "SPECIES='%s'\n", $opts{'sp'}; printf $FH "ASSEMBLY='%s'\n", $opts{'as'}; From 9ebe0472039dc8978ac3a3c329399d292b1cc848 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Sun, 18 Feb 2018 17:18:55 +0000 Subject: [PATCH 15/57] Fixed up some issues in use of R libs and cleanup of data --- Dockerfile | 15 ++++++++++++--- build/opt-build.sh | 2 +- scripts/analysisWGS.sh | 10 +++++++--- scripts/ds-cgpwgs.pl | 3 ++- 4 files changed, 22 insertions(+), 8 deletions(-) diff --git a/Dockerfile b/Dockerfile index 312e8ab..475b360 100644 --- a/Dockerfile +++ b/Dockerfile @@ -34,7 +34,7 @@ RUN locale-gen en_US.UTF-8 RUN update-locale LANG=en_US.UTF-8 ENV OPT /opt/wtsi-cgp -ENV PATH $OPT/bin:$PATH +ENV PATH $OPT/bin:$OPT/biobambam2/bin:$PATH ENV PERL5LIB $OPT/lib/perl5 ENV LD_LIBRARY_PATH $OPT/lib ENV LC_ALL en_US.UTF-8 @@ -56,6 +56,13 @@ LABEL vendor="Cancer Genome Project, Wellcome Trust Sanger Institute" LABEL uk.ac.sanger.cgp.description="CGP WGS pipeline for dockstore.org" LABEL uk.ac.sanger.cgp.version="2.0.0-rc1" +RUN apt-get update +RUN apt-get install -qy --no-install-recommends lsb-release + +RUN echo "deb http://cran.rstudio.com/bin/linux/ubuntu `lsb_release -cs`/" >> /etc/apt/sources.list +RUN gpg --keyserver keyserver.ubuntu.com --recv-key E084DAB9 +RUN gpg -a --export E084DAB9 | apt-key add - + RUN apt-get -yq update RUN apt-get install -yq --no-install-recommends\ apt-transport-https\ @@ -72,13 +79,15 @@ RUN apt-get install -yq --no-install-recommends\ libcairo2\ gfortran\ r-base\ - exonerate + exonerate\ + libboost-iostreams-dev\ + p11-kit RUN locale-gen en_US.UTF-8 RUN update-locale LANG=en_US.UTF-8 ENV OPT /opt/wtsi-cgp -ENV PATH $OPT/bin:$PATH +ENV PATH $OPT/bin:$OPT/biobambam2/bin:$PATH ENV PERL5LIB $OPT/lib/perl5 ENV LD_LIBRARY_PATH $OPT/lib ENV LC_ALL en_US.UTF-8 diff --git a/build/opt-build.sh b/build/opt-build.sh index 578c7ed..c24f2b5 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -12,7 +12,7 @@ set -u VER_ALLELECOUNT="feature/overlapping_reads" ### cgpNgsQc -VER_CGPNGSQC="v1.4.0" +VER_CGPNGSQC="feature/cramAndCleanup" VER_VERIFYBAM="1.1.2" ### ascatNgs diff --git a/scripts/analysisWGS.sh b/scripts/analysisWGS.sh index a68b2fb..0e5d653 100755 --- a/scripts/analysisWGS.sh +++ b/scripts/analysisWGS.sh @@ -231,6 +231,7 @@ do_parallel[CaVEMan_split]="caveman.pl \ -f $REF_BASE/caveman/flagging/flag.to.vcf.convert.ini \ -e $CAVESPLIT \ -o $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/caveman \ + -x $CONTIG_EXCLUDE \ -p split" echo "Starting Parallel block 2: `date`" @@ -343,6 +344,7 @@ do_parallel[CaVEMan]="caveman.pl \ -f $REF_BASE/caveman/flagging/flag.to.vcf.convert.ini \ -e $CAVESPLIT \ -o $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/caveman \ + -x $CONTIG_EXCLUDE \ -no-flagging" echo "Starting Parallel block 4: `date`" @@ -375,7 +377,7 @@ do_parallel[BRASS]="brass.pl -j 4 -k 4 -c $CPU \ -cb $REF_BASE/brass/cytoband.txt \ -t $BAM_MT_TMP \ -n $BAM_WT_TMP \ - -ss $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/ascat/*.samplestatistics.txt \ + -ss $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/ascat/$NAME_MT.samplestatistics.txt \ -o $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/brass" # ensure no annotated pindel @@ -440,13 +442,15 @@ rm -f $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/battenberg/tmpBattenberg/ mv $OUTPUT_DIR/timings/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.time.verify_WT $OUTPUT_DIR/timings/${PROTOCOL}_${NAME_WT}.time.verify_WT mv $OUTPUT_DIR/timings/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.time.verify_MT $OUTPUT_DIR/timings/${PROTOCOL}_${NAME_MT}.time.verify_MT -# cleanup reference area, see ds-cgpwxs.pl +# cleanup reference area, see ds-cgpwgs.pl if [ ! -z ${CLEAN_REF+x} ]; then rm -rf $REF_BASE fi # cleanup ref cache -rm -rf $USER_CACHE +if [ "$ALN_EXTN" == "cram" ]; then + rm -rf $USER_CACHE +fi echo 'Package results' # timings first diff --git a/scripts/ds-cgpwgs.pl b/scripts/ds-cgpwgs.pl index 7cafbd0..e375bfd 100644 --- a/scripts/ds-cgpwgs.pl +++ b/scripts/ds-cgpwgs.pl @@ -138,7 +138,8 @@ print $R_FH qq{options(bitmapType='cairo')\n}; close $R_FH; -exec('analysisWGS.sh', $run_file); # I will never return to the perl code +my $cmd = sprintf '/usr/bin/time -o %s/WGS_%s_vs_%s.time -v /opt/wtsi-cgp/bin/analysisWGS.sh %s', $opts{'o'}, $opts{'mt_sm'}, $opts{'wt_sm'}, $run_file; +exec($cmd); # I will never return to the perl code sub add_species_flag_ini { my ($species, $ini_in) = @_; From b0928c17b51f8ca23ce95a98fb5a2281bab8e330 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Sun, 18 Feb 2018 18:51:05 +0000 Subject: [PATCH 16/57] Upgrade and patch ascat.R --- build/opt-build.sh | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/build/opt-build.sh b/build/opt-build.sh index c24f2b5..cdf16a5 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -16,8 +16,8 @@ VER_CGPNGSQC="feature/cramAndCleanup" VER_VERIFYBAM="1.1.2" ### ascatNgs -VER_ASCATNGS="v4.1.0" -SRC_ASCAT="https://raw.githubusercontent.com/Crick-CancerGenomics/ascat/6d40e69a2919ddfc1cda870310203c772bf846ce/ASCAT/R/ascat.R" +VER_ASCATNGS="feature/patchAndUpgradeAscat" +SRC_ASCAT="https://raw.githubusercontent.com/Crick-CancerGenomics/ascat/v2.5/ASCAT/R/ascat.R" ### grass VER_GRASS="v2.1.0" @@ -118,7 +118,7 @@ if [ ! -e $SETUP_DIR/ascatNgs.success ]; then # add ascatSrc curl -sSL $SRC_ASCAT > share/ascat/ascat.R - + patch share/ascat/ascat.R ../patches/ascat_singleSnp.patch cpanm --no-interactive --notest --mirror http://cpan.metacpan.org --notest -l $INST_PATH --installdeps . cpanm -v --no-interactive --mirror http://cpan.metacpan.org -l $INST_PATH . cd $SETUP_DIR From 0a922bce6cc1ad5802a59b0369459dda64ef45a1 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 14 Mar 2018 14:38:16 +0000 Subject: [PATCH 17/57] allow setting of ascat purity/ploidy --- cwls/cgpwgs.cwl | 35 ++++++++++++++--- scripts/analysisWGS.sh | 29 +++++++++----- scripts/ds-cgpwgs.pl | 86 +++++++++++++++++++++++++++--------------- 3 files changed, 106 insertions(+), 44 deletions(-) diff --git a/cwls/cgpwgs.cwl b/cwls/cgpwgs.cwl index 4b4d1ba..df410dc 100644 --- a/cwls/cgpwgs.cwl +++ b/cwls/cgpwgs.cwl @@ -23,11 +23,6 @@ doc: | WARNING: The usual setting for 'exclude' is 'NC_007605,hs37d5,GL%' (human GRCh37/NCBI37). Examples are configured to run as quickly as possible. -dct:creator: - "@id": "http://orcid.org/0000-0002-5634-1539" - foaf:name: Keiran M Raine - foaf:mbox: "keiranmraine@gmail.com" - requirements: - class: DockerRequirement dockerPull: "quay.io/wtsicgp/dockstore-cgpwgs:2.0.0-rc1" @@ -163,6 +158,21 @@ inputs: position: 14 separate: true + purity: + type: float? + doc: "Set the purity (rho) for ascat when default solution needs additional guidance. If set ploidy is also required." + inputBinding: + prefix: -pu + separate: true + + ploidy: + type: float? + doc: "Set the ploidy (psi) for ascat when default solution needs additional guidance. If set purity is also required." + inputBinding: + prefix: -pl + separate: true + + outputs: run_params: type: File @@ -186,3 +196,18 @@ outputs: glob: WGS_*_vs_*.time baseCommand: ["/opt/wtsi-cgp/bin/ds-cgpwgs.pl"] + +$schemas: + - http://schema.org/docs/schema_org_rdfa.html + +$namespaces: + s: http://schema.org/ + +s:codeRepository: https://github.com/cancerit/dockstore-cgpwgs +s:license: https://spdx.org/licenses/AGPL-3.0-only + +s:author: + - class: s:Person + s:identifier: https://orcid.org/0000-0002-5634-1539 + s:email: mailto:cgphelp@sanger.ac.uk + s:name: Keiran Raine diff --git a/scripts/analysisWGS.sh b/scripts/analysisWGS.sh index 0e5d653..46b5202 100755 --- a/scripts/analysisWGS.sh +++ b/scripts/analysisWGS.sh @@ -63,10 +63,6 @@ fi source $PARAM_FILE env -TMP=$OUTPUT_DIR/tmp -mkdir -p $TMP -mkdir -p $OUTPUT_DIR/timings - if [ -z ${CPU+x} ]; then CPU=`grep -c ^processor /proc/cpuinfo` fi @@ -78,7 +74,9 @@ echo -e "\tBAM_MT : $BAM_MT" echo -e "\tBAM_WT : $BAM_WT" set -u -mkdir -p $OUTPUT_DIR +TMP=$OUTPUT_DIR/tmp +mkdir -p $TMP +mkdir -p $OUTPUT_DIR/timings ## get sample names from BAM headers NAME_MT=`samtools view -H $BAM_MT | perl -ne 'chomp; if($_ =~ m/^\@RG/) {($sm) = $_ =~m/\tSM:([^\t]+)/; print "$sm\n";}' | uniq` @@ -116,6 +114,16 @@ ln -fs $BAM_WT $BAM_WT_TMP ln -fs $BAM_WT.bas $BAM_WT_TMP.bas ln -fs $IDX_WT $IDX_WT_TMP +ASCAT_ADD_ARGS = '' +# ASCAT_PURITY set +if [ ! -z ${ASCAT_PURITY+x} ]; then + ASCAT_ADD_ARGS="$ASCAT_ADD_ARGS -pu $ASCAT_PURITY" +fi +# ASCAT_PLOIDY set +if [ ! -z ${ASCAT_PLOIDY+x} ]; then + ASCAT_ADD_ARGS="$ASCAT_ADD_ARGS -pl $ASCAT_PLOIDY" +fi + ## Make fake copynumber so we can run early steps of caveman perl -alne 'print join(qq{\t},$F[0],0,$F[1],2);' < $REF_BASE/genome.fa.fai | tee $TMP/norm.cn.bed > $TMP/tum.cn.bed @@ -182,6 +190,7 @@ echo -e "\t[Parallel block 1] VerifyBam Normal added..." do_parallel[verify_WT]="verifyBamHomChk.pl -d 25 \ -o $OUTPUT_DIR/${PROTOCOL}_${NAME_WT}/contamination \ -b $BAM_WT_TMP \ + -t $CPU \ -j $OUTPUT_DIR/${PROTOCOL}_${NAME_WT}/contamination/result.json" echo "Starting Parallel block 1: `date`" @@ -255,7 +264,8 @@ do_parallel[ascat]="ascat.pl \ -ra $ASSEMBLY \ -pr $PROTOCOL \ -pl ILLUMINA \ - -c $CPU" + -c $CPU\ + $ASCAT_ADD_ARGS" echo -e "\t[Parallel block 3] BRASS_input added..." do_parallel[BRASS_input]="brass.pl -j 4 -k 4 -c $CPU \ @@ -426,6 +436,7 @@ echo -e "\t[Parallel block 6] VerifyBam Tumour added..." do_parallel[verify_MT]="verifyBamHomChk.pl -d 25 \ -o $OUTPUT_DIR/${PROTOCOL}_$NAME_MT/contamination \ -b $BAM_MT_TMP \ + -t $CPU \ -a $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/ascat/${NAME_MT}.copynumber.caveman.csv \ -j $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}/contamination/result.json" @@ -454,8 +465,8 @@ fi echo 'Package results' # timings first -tar -C $OUTPUT_DIR -zcf ${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.timings.tar.gz timings -tar -C $OUTPUT_DIR -zcf ${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.result.tar.gz ${PROTOCOL}_${NAME_MT}_vs_${NAME_WT} ${PROTOCOL}_${NAME_MT} ${PROTOCOL}_${NAME_WT} -cp $PARAM_FILE ${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.run.params +tar -C $OUTPUT_DIR -zcf $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.timings.tar.gz timings +tar -C $OUTPUT_DIR -zcf $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.result.tar.gz ${PROTOCOL}_${NAME_MT}_vs_${NAME_WT} ${PROTOCOL}_${NAME_MT} ${PROTOCOL}_${NAME_WT} +cp $PARAM_FILE $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.run.params echo -e "\nWorkflow end: `date`" diff --git a/scripts/ds-cgpwgs.pl b/scripts/ds-cgpwgs.pl index e375bfd..4b07f42 100644 --- a/scripts/ds-cgpwgs.pl +++ b/scripts/ds-cgpwgs.pl @@ -16,6 +16,8 @@ 'o' => $ENV{HOME}, 'sp' => undef, 'as' => undef, + 'pu' => undef, + 'pl' => undef, ); GetOptions( 'h|help' => \$opts{'h'}, @@ -36,45 +38,23 @@ 'cr|cavereads=i' => \$opts{'cr'}, 'c|cores:i' => \$opts{'c'}, 'o|outdir:s' => \$opts{'o'}, + 'pu|purity:f' => \$opts{'pu'}, + 'pl|ploidy:f' => \$opts{'pl'}, ) or pod2usage(2); pod2usage(-verbose => 1, -exitval => 0) if(defined $opts{'h'}); pod2usage(-verbose => 2, -exitval => 0) if(defined $opts{'m'}); +if((defined($opts{'pu'}) && !defined($opts{'pl'})) || (!defined($opts{'pu'}) && defined($opts{'pl'}))) { + pod2usage(-msg => "\nERROR: If one of purity/ploidy are defined, both should be defined.\n", + -verbose => 1, -output => \*STDERR); +} + delete $opts{'h'}; delete $opts{'m'}; -delete $opts{'sp'} if(! defined $opts{'sp'} || length $opts{'sp'} == 0); -delete $opts{'as'} if(! defined $opts{'as'} || length $opts{'as'} == 0); delete $opts{'sb'} if(! defined $opts{'sb'} || length $opts{'sb'} == 0); -## read species/assembly from bam headers -my ($mt_species, $mt_assembly) = species_assembly_from_xam($opts{'t'}); -my ($wt_species, $wt_assembly) = species_assembly_from_xam($opts{'n'}); -if($mt_species ne $wt_species) { - warn "WARN: Species mismatch between T/N [CR|B]AM headers\n"; - if(!defined $opts{'sp'}) { - die "ERROR: Please define species to handle this mismatch\n"; - } -} -elsif($mt_species ne q{}) { - $opts{'sp'} = $mt_species; -} -if(!defined $opts{'sp'}) { - die "ERROR: Please define species, not found in [CR|B]AM headers.\n"; -} - -if($mt_assembly ne $wt_assembly) { - warn "WARN: Assembly mismatch between T/N [CR|B]AM headers\n"; - if(!defined $opts{'as'}) { - die "ERROR: Please define assembly to handle this mismatch\n"; - } -} -elsif($mt_assembly ne q{}) { - $opts{'as'} = $mt_assembly; -} -if(!defined $opts{'as'}) { - die "ERROR: Please define assembly, not found in [CR|B]AM headers.\n"; -} +resolve_sp_as(\%opts); $opts{'mt_sm'} = sample_name_from_xam($opts{'t'}); $opts{'wt_sm'} = sample_name_from_xam($opts{'n'}); @@ -128,6 +108,8 @@ printf $FH "CAVESPLIT='%s'\n", $opts{'cr'}; printf $FH "SNVFLAG='%s'\n", $ini; printf $FH "CPU=%d\n", $opts{'c'} if(defined $opts{'c'}); +printf $FH "ASCAT_PLOIDY=%.5f\n", $opts{'pl'} if(defined $opts{'pl'}); +printf $FH "ASCAT_PURITY=%.5f\n", $opts{'pu'} if(defined $opts{'pu'}); printf $FH "CLEAN_REF=1\n" if($ref_unpack); # Options to disable algorithms print $FH "SKIPBB=1\n" if(exists $opts{'sb'}); @@ -141,6 +123,38 @@ my $cmd = sprintf '/usr/bin/time -o %s/WGS_%s_vs_%s.time -v /opt/wtsi-cgp/bin/analysisWGS.sh %s', $opts{'o'}, $opts{'mt_sm'}, $opts{'wt_sm'}, $run_file; exec($cmd); # I will never return to the perl code +sub resolve_sp_as { + my $options = shift; + ## read species/assembly from bam headers + my ($mt_species, $mt_assembly) = species_assembly_from_xam($options->{'t'}); + my ($wt_species, $wt_assembly) = species_assembly_from_xam($options->{'n'}); + if($mt_species ne $wt_species) { + warn "WARN: Species mismatch between T/N [CR|B]AM headers\n"; + if(!defined $options->{'sp'} || $options->{'sp'} eq q{}) { + die "ERROR: Please define species to handle this mismatch\n"; + } + } + elsif($mt_species ne q{}) { + $options->{'sp'} = $mt_species; + } + if(!defined $options->{'sp'} || $options->{'sp'} eq q{}) { + die "ERROR: Please define species, not found in [CR|B]AM headers.\n"; + } + + if($mt_assembly ne $wt_assembly) { + warn "WARN: Assembly mismatch between T/N [CR|B]AM headers\n"; + if(!defined $options->{'as'} || $options->{'as'} eq q{}) { + die "ERROR: Please define assembly to handle this mismatch\n"; + } + } + elsif($mt_assembly ne q{}) { + $options->{'as'} = $mt_assembly; + } + if(!defined $options->{'as'} || $options->{'as'} eq q{}) { + die "ERROR: Please define assembly, not found in [CR|B]AM headers.\n"; + } +} + sub add_species_flag_ini { my ($species, $ini_in) = @_; $species =~ s/ /_/g; @@ -248,6 +262,8 @@ =head1 SYNOPSIS -skipbb -sb Skip Battenberg allele counts -outdir -o Set the output folder [$HOME] -cores -c Set the number of cpu/cores available [default all]. + -ploidy -pl Set the ploidy (rho) for ascat - requires purity + -purity -pu Set the purity (psi) for ascat - requires ploidy Other: -help -h Brief help message. @@ -318,6 +334,16 @@ =head1 OPTION DETAILS Sets the number of cores to be used during processing. Default to use all at appropriate points in analysis. +=item B<-ploidy> + +Set the ploidy (psi) for ascat when default solution needs additional guidance. If set B<-purity> +is also required. + +=item B<-purity> + +Set the purity (rho) for ascat when default solution needs additional guidance. If set B<-ploidy> +is also required. + =back =cut From 1d040f1271c173c867f1a1798629522fb87dd2b4 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 14 Mar 2018 14:38:32 +0000 Subject: [PATCH 18/57] moved --- Dockstore.cwl | 1 - 1 file changed, 1 deletion(-) delete mode 120000 Dockstore.cwl diff --git a/Dockstore.cwl b/Dockstore.cwl deleted file mode 120000 index 3d7d01f..0000000 --- a/Dockstore.cwl +++ /dev/null @@ -1 +0,0 @@ -cwls/cgpwgs.cwl \ No newline at end of file From dd591f0e8fabd50ed9983f5425366bbe3c754eaa Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 14 Mar 2018 14:38:58 +0000 Subject: [PATCH 19/57] Show use of purity/ploidy --- examples/cgpwgs/bam_bai_pu_pl.json | 61 ++++++++++++++++++++++++++++++ 1 file changed, 61 insertions(+) create mode 100644 examples/cgpwgs/bam_bai_pu_pl.json diff --git a/examples/cgpwgs/bam_bai_pu_pl.json b/examples/cgpwgs/bam_bai_pu_pl.json new file mode 100644 index 0000000..5bb7cb6 --- /dev/null +++ b/examples/cgpwgs/bam_bai_pu_pl.json @@ -0,0 +1,61 @@ +{ + "reference": { + "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/core_ref_GRCh37d5.tar.gz", + "class": "File" + }, + "annot": { + "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/VAGrENT_ref_GRCh37d5_ensembl_75.tar.gz", + "class": "File" + }, + "snv_indel": { + "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/SNV_INDEL_ref_GRCh37d5-fragment.tar.gz", + "class": "File" + }, + "cnv_sv": { + "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/CNV_SV_ref_GRCh37d5_brass6+.tar.gz", + "class": "File" + }, + "subcl": { + "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/SUBCL_ref_GRCh37d5.tar.gz", + "class": "File" + }, + "tumour": { + "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/12x/COLO-829.bam", + "class": "File" + }, + "tumourIdx": { + "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/12x/COLO-829.bam.bai", + "class": "File" + }, + "normal": { + "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/12x/COLO-829-BL.bam", + "class": "File" + }, + "normalIdx": { + "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/12x/COLO-829-BL.bam.bai", + "class": "File" + }, + "exclude": "1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,22,X,Y,MT,NC_007605,hs37d5,GL%", + "species": "human", + "assembly": "GRCh37d5", + "cavereads": 800000, + "skipbb": true, + "purity": 0.99, + "ploidy": 2, + "result_archive": { + "path": "/tmp/result_WGS.tar.gz", + "class": "File" + }, + "timings": { + "path": "/tmp/timings_WGS.tar.gz", + "class": "File" + }, + "global_time": { + "path": "/tmp/global_WGS.time", + "class": "File" + }, + "run_params": { + "path": "/tmp/params_WGS.params", + "class": "File" + } +} From e779158c87ecf9f5a7b95346400c9ea181ddb575 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 14 Mar 2018 14:39:41 +0000 Subject: [PATCH 20/57] ASCAT core has been patched in main repo, also depend on apt version of exonerate now --- build/opt-build.sh | 28 ++++++---------------------- 1 file changed, 6 insertions(+), 22 deletions(-) diff --git a/build/opt-build.sh b/build/opt-build.sh index cdf16a5..126e97c 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -9,21 +9,21 @@ fi set -u ### alleleCount -VER_ALLELECOUNT="feature/overlapping_reads" +VER_ALLELECOUNT="v4.0.0" ### cgpNgsQc -VER_CGPNGSQC="feature/cramAndCleanup" -VER_VERIFYBAM="1.1.2" +VER_CGPNGSQC="v1.5.0" +VER_VERIFYBAM="1.1.3" ### ascatNgs -VER_ASCATNGS="feature/patchAndUpgradeAscat" -SRC_ASCAT="https://raw.githubusercontent.com/Crick-CancerGenomics/ascat/v2.5/ASCAT/R/ascat.R" +VER_ASCATNGS="v4.2.0" +SRC_ASCAT="https://raw.githubusercontent.com/Crick-CancerGenomics/ascat/v2.5.1/ASCAT/R/ascat.R" ### grass VER_GRASS="v2.1.0" ### BRASS -VER_BRASS="v6.0.6" +VER_BRASS="feature/cleanup" SOURCE_BLAT="http://users.soe.ucsc.edu/~kent/src/blatSrc35.zip" SRC_FASTA36="https://github.com/wrpearson/fasta36/archive/v36.3.8d_13Apr16.tar.gz" @@ -118,7 +118,6 @@ if [ ! -e $SETUP_DIR/ascatNgs.success ]; then # add ascatSrc curl -sSL $SRC_ASCAT > share/ascat/ascat.R - patch share/ascat/ascat.R ../patches/ascat_singleSnp.patch cpanm --no-interactive --notest --mirror http://cpan.metacpan.org --notest -l $INST_PATH --installdeps . cpanm -v --no-interactive --mirror http://cpan.metacpan.org -l $INST_PATH . cd $SETUP_DIR @@ -194,21 +193,6 @@ if [ ! -e $SETUP_DIR/BRASS.success ]; then touch $SETUP_DIR/velvet.success fi - # if [ ! -e $SETUP_DIR/exonerate.success ]; then - # cd $SETUP_DIR/distro/distros - # tar zxf exonerate-2.2.0.tar.gz - # cd exonerate-2.2.0 - # cp ../patches/exonerate_pthread-asneeded.diff . - # patch -p1 < exonerate_pthread-asneeded.diff - # ./configure --prefix=$INST_PATH - # make # don't do multi-threaded make - # make check - # make install - # cd $SETUP_DIR/distro - # rm -rf distros/exonerate-2.2.0 - # touch $SETUP_DIR/exonerate.success - # fi - cpanm --no-interactive --notest --mirror http://cpan.metacpan.org -l $INST_PATH Graph cpanm --no-interactive --notest --mirror http://cpan.metacpan.org -l $INST_PATH Bio::Tools::Run::WrapperBase From bdb99ba79db6ef0647d354fb93389a48652dd403 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 14 Mar 2018 14:39:59 +0000 Subject: [PATCH 21/57] Cleaned up the readme a small amount and changes to travis build, expect some cycles here --- .travis.yml | 35 ++++++++++++++++++++++++++++++++++- README.md | 2 ++ 2 files changed, 36 insertions(+), 1 deletion(-) diff --git a/.travis.yml b/.travis.yml index efb916e..70afac0 100644 --- a/.travis.yml +++ b/.travis.yml @@ -8,7 +8,40 @@ services: - docker install: - - docker build -t dockstore-cgpwgs . + - virtualenv -p python3 venv + - source venv/bin/activate + - pip install html5lib cwltool script: + - set -e + - echo 'Validate CWL file(s)' + - cwltool --validate cwls/cgpwxs.cwl + - echo 'Build and check docker image' + - docker build -t dockstore-cgpwgs . - docker images | grep -c dockstore-cgpwgs + - echo 'Verify program from each inherited package is found (dockstore-cgpbigwig)' + - docker run -ti --rm dockstore-cgpmap bwjoin --version + - echo 'Verify program from each inherited package is found (dockstore-cgpmap)' + - docker run -ti --rm dockstore-cgpwgs ds-cgpmap.pl -h + - docker run -ti --rm dockstore-cgpwgs bwa_mem.pl -version + - docker run -ti --rm dockstore-cgpwgs bammarkduplicates2 --version + - docker run -ti --rm dockstore-cgpmap samtools --version + - docker run -ti --rm dockstore-cgpwgs bash -c 'bwa 2>&1 | grep Version' + - echo 'Verify program from each inherited package is found (dockstore-cgpwxs)' + - docker run -ti --rm dockstore-cgpwgs ds-cgpwxs.pl -h + - docker run -ti --rm dockstore-cgpwgs cgpVCFSplit.pl -version + - docker run -ti --rm dockstore-cgpwgs vcftools --version + - docker run -ti --rm dockstore-cgpwgs bedtools --version + - docker run -ti --rm dockstore-cgpwgs bash -c 'pindel 2>&1 | grep Welcome' + - docker run -ti --rm dockstore-cgpwgs pindel.pl -version + - docker run -ti --rm dockstore-cgpwgs caveman --version + - docker run -ti --rm dockstore-cgpwgs caveman.pl -version + - docker run -ti --rm dockstore-cgpwgs cgpFlagCaVEMan.pl -version + - docker run -ti --rm dockstore-cgpwgs AnnotateVcf.pl -version + - echo 'Verify a program from each new package is found (dockstore-cgpwgs)' + - docker run -ti --rm dockstore-cgpwgs ds-cgpwgs.pl -h + - docker run -ti --rm dockstore-cgpmap bash -c 'verifyBamId 2>&1 | grep verifyBamID' + - docker run -ti --rm dockstore-cgpwgs alleleCount --version + - docker run -ti --rm dockstore-cgpwgs brass.pl -version + - docker run -ti --rm dockstore-cgpwgs ascat.pl -version + - docker run -ti --rm dockstore-cgpwgs battenberg.pl -version diff --git a/README.md b/README.md index 6e90d43..5ddf730 100644 --- a/README.md +++ b/README.md @@ -12,6 +12,8 @@ the [Dockstore.org](https://dockstore.org/) framework. | --------------------------------------------- | ----------------------------------------------- | | [![Master Badge][travis-master]][travis-base] | [![Develop Badge][travis-develop]][travis-base] | + + ## Usage This is intended to be run using the Dockstore.org framework under docker but can be executed as a From 521be98572348e8da365a4703dc91b5e76509292 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 14 Mar 2018 14:53:19 +0000 Subject: [PATCH 22/57] Silly mistake --- .travis.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.travis.yml b/.travis.yml index 70afac0..7d95366 100644 --- a/.travis.yml +++ b/.travis.yml @@ -15,7 +15,7 @@ install: script: - set -e - echo 'Validate CWL file(s)' - - cwltool --validate cwls/cgpwxs.cwl + - cwltool --validate cwls/cgpwgs.cwl - echo 'Build and check docker image' - docker build -t dockstore-cgpwgs . - docker images | grep -c dockstore-cgpwgs From 0db2608a40068624a012e372a6fe4d5958972c0b Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 14 Mar 2018 15:20:16 +0000 Subject: [PATCH 23/57] Image for wxs has been released --- Dockerfile | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/Dockerfile b/Dockerfile index 475b360..994d493 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM quay.io/wtsicgp/dockstore-cgpwxs:3.0.0-rc2 as builder +FROM quay.io/wtsicgp/dockstore-cgpwxs:3.0.0 as builder USER root From 8087e328c6f6db66d0c6584329fdfa021ba2ce18 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 14 Mar 2018 16:17:24 +0000 Subject: [PATCH 24/57] Fix image referencing --- .travis.yml | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/.travis.yml b/.travis.yml index 7d95366..d0f7e4a 100644 --- a/.travis.yml +++ b/.travis.yml @@ -25,7 +25,7 @@ script: - docker run -ti --rm dockstore-cgpwgs ds-cgpmap.pl -h - docker run -ti --rm dockstore-cgpwgs bwa_mem.pl -version - docker run -ti --rm dockstore-cgpwgs bammarkduplicates2 --version - - docker run -ti --rm dockstore-cgpmap samtools --version + - docker run -ti --rm dockstore-cgpwgs samtools --version - docker run -ti --rm dockstore-cgpwgs bash -c 'bwa 2>&1 | grep Version' - echo 'Verify program from each inherited package is found (dockstore-cgpwxs)' - docker run -ti --rm dockstore-cgpwgs ds-cgpwxs.pl -h @@ -40,7 +40,7 @@ script: - docker run -ti --rm dockstore-cgpwgs AnnotateVcf.pl -version - echo 'Verify a program from each new package is found (dockstore-cgpwgs)' - docker run -ti --rm dockstore-cgpwgs ds-cgpwgs.pl -h - - docker run -ti --rm dockstore-cgpmap bash -c 'verifyBamId 2>&1 | grep verifyBamID' + - docker run -ti --rm dockstore-cgpwgs bash -c 'verifyBamId 2>&1 | grep verifyBamID' - docker run -ti --rm dockstore-cgpwgs alleleCount --version - docker run -ti --rm dockstore-cgpwgs brass.pl -version - docker run -ti --rm dockstore-cgpwgs ascat.pl -version From 07bc00dc592485dd0623ff142e766082823e1bff Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 14 Mar 2018 16:37:36 +0000 Subject: [PATCH 25/57] Missed a reference to cgpmap --- .travis.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.travis.yml b/.travis.yml index d0f7e4a..6fd5d4b 100644 --- a/.travis.yml +++ b/.travis.yml @@ -20,7 +20,7 @@ script: - docker build -t dockstore-cgpwgs . - docker images | grep -c dockstore-cgpwgs - echo 'Verify program from each inherited package is found (dockstore-cgpbigwig)' - - docker run -ti --rm dockstore-cgpmap bwjoin --version + - docker run -ti --rm dockstore-cgpwgs bwjoin --version - echo 'Verify program from each inherited package is found (dockstore-cgpmap)' - docker run -ti --rm dockstore-cgpwgs ds-cgpmap.pl -h - docker run -ti --rm dockstore-cgpwgs bwa_mem.pl -version From ac219406d19a178b95cf73d5722377f380925fe4 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 14 Mar 2018 19:09:42 +0000 Subject: [PATCH 26/57] User setup seems to have been lost in switch to build stages --- Dockerfile | 3 +++ 1 file changed, 3 insertions(+) diff --git a/Dockerfile b/Dockerfile index 994d493..dedd32c 100644 --- a/Dockerfile +++ b/Dockerfile @@ -102,6 +102,9 @@ COPY scripts/analysisWGS.sh $OPT/bin/analysisWGS.sh COPY scripts/ds-cgpwgs.pl $OPT/bin/ds-cgpwgs.pl RUN chmod a+x $OPT/bin/analysisWGS.sh $OPT/bin/ds-cgpwgs.pl +## USER CONFIGURATION +RUN adduser --disabled-password --gecos '' ubuntu && chsh -s /bin/bash && mkdir -p /home/ubuntu + USER ubuntu WORKDIR /home/ubuntu From ed42d2226b509057fa63e20b0c1c6109a32a8988 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 14 Mar 2018 19:09:57 +0000 Subject: [PATCH 27/57] Removed redundant rprofile bit --- scripts/ds-cgpwgs.pl | 3 --- 1 file changed, 3 deletions(-) diff --git a/scripts/ds-cgpwgs.pl b/scripts/ds-cgpwgs.pl index 4b07f42..8d2e2c1 100644 --- a/scripts/ds-cgpwgs.pl +++ b/scripts/ds-cgpwgs.pl @@ -83,9 +83,6 @@ ## now complete the caveman flaging file correctly my $ini = add_species_flag_ini($opts{'sp'}, "$ref_area/caveman/flag.vcf.config.WGS.ini"); -## CWL changes $HOME so need to copy in relevant config for R -copy('/home/ubuntu/.Rprofile', $ENV{HOME}.'/.Rprofile'); - my $run_file = $opts{'o'}.'/run.params'; open my $FH,'>',$run_file or die "Failed to write to $run_file: $!"; # Force explicit checking of file flush From 52d764de932f59a45e718ae172b0b19adfb6d83d Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Fri, 16 Mar 2018 04:17:03 +0000 Subject: [PATCH 28/57] Doc updates [ci skip] --- CHANGES.md | 4 ++-- README.md | 22 ++++++++++++++++------ 2 files changed, 18 insertions(+), 8 deletions(-) diff --git a/CHANGES.md b/CHANGES.md index a17d011..860555a 100644 --- a/CHANGES.md +++ b/CHANGES.md @@ -3,8 +3,8 @@ ## 2.0.0 * Reorganisation to allow access of other tools or necessary bindings -* See dockstore-cgpmap 3.0.0 (primarily adds mismatchQc) -* See dockstore-cgpwxs 3.0.0 (fragment based analysis) +* See dockstore-cgpmap 3.0.0 - 3.0.2 (primarily adds mismatchQc and threads to some tools) +* See dockstore-cgpwxs 3.0.0 - 3.0.1 (fragment based analysis) * You will need to update the reference pack to include the new flagging rules, see example `json` files * Using build stages to shrink images. diff --git a/README.md b/README.md index 5ddf730..0dbfb1a 100644 --- a/README.md +++ b/README.md @@ -12,7 +12,16 @@ the [Dockstore.org](https://dockstore.org/) framework. | --------------------------------------------- | ----------------------------------------------- | | [![Master Badge][travis-master]][travis-base] | [![Develop Badge][travis-develop]][travis-base] | + +- [Usage](#usage) + - [Usable Cores](#usable-cores) +- [Example data](#example-data) +- [Diagram of internals](#diagram-of-internals) +- [Release process](#release-process) +- [LICENCE](#licence) + + ## Usage @@ -69,12 +78,13 @@ handled by a Dockstore run. This project is maintained using HubFlow. 1. Make appropriate changes -2. Bump version in `Dockerfile` and `Dockstore.cwl` -3. Push changes -4. Check state on Travis -5. Generate the release (add notes to GitHub) -6. Confirm that image has been built on [quay.io][quay-builds] -7. Update the [dockstore][dockstore-cgpwgs] entry, see [their docs][dockstore-get-started]. +1. Bump version in `Dockerfile` and `Dockstore.cwl` +1. Push changes +1. Check state on Travis +1. Update expected datasets +1. Generate the release (add notes to GitHub) +1. Confirm that image has been built on [quay.io][quay-builds] +1. Update the [dockstore][dockstore-cgpwgs] entry, see [their docs][dockstore-get-started]. ## LICENCE From 638a5d34d52fef5ab3ef4474dd947182c147456e Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Fri, 16 Mar 2018 04:46:14 +0000 Subject: [PATCH 29/57] Install R deps --- build/opt-build.sh | 8 +++++--- 1 file changed, 5 insertions(+), 3 deletions(-) diff --git a/build/opt-build.sh b/build/opt-build.sh index 126e97c..b00a3b9 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -23,12 +23,12 @@ SRC_ASCAT="https://raw.githubusercontent.com/Crick-CancerGenomics/ascat/v2.5.1/A VER_GRASS="v2.1.0" ### BRASS -VER_BRASS="feature/cleanup" +VER_BRASS="v6.1.0" SOURCE_BLAT="http://users.soe.ucsc.edu/~kent/src/blatSrc35.zip" SRC_FASTA36="https://github.com/wrpearson/fasta36/archive/v36.3.8d_13Apr16.tar.gz" ### cgpBattenberg -VER_CGPBB="v3.1.0" +VER_CGPBB="v3.2.2" if [ "$#" -lt "1" ] ; then @@ -224,7 +224,9 @@ if [ ! -e $SETUP_DIR/cgpBB.success ]; then curl -sSL --retry 10 https://github.com/cancerit/cgpBattenberg/archive/${VER_CGPBB}.tar.gz > distro.tar.gz rm -rf distro/* tar --strip-components 1 -C distro -xzf distro.tar.gz - cd distro/perl + cd distro/Rsupport + ./setupR.sh $INST_PATH + cd $SETUP_DIR/distro/perl cpanm --no-interactive --notest --mirror http://cpan.metacpan.org --notest -l $INST_PATH --installdeps . cpanm -v --no-interactive --mirror http://cpan.metacpan.org -l $INST_PATH . cd $SETUP_DIR From 2177701b49738b7aef396b9e25a424594dbc0504 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Fri, 16 Mar 2018 04:46:39 +0000 Subject: [PATCH 30/57] Shrink output and versions --- Dockerfile | 34 ++++++++++++++++++---------------- cwls/cgpwgs.cwl | 2 +- 2 files changed, 19 insertions(+), 17 deletions(-) diff --git a/Dockerfile b/Dockerfile index dedd32c..f9f7d2b 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,16 +1,16 @@ -FROM quay.io/wtsicgp/dockstore-cgpwxs:3.0.0 as builder +FROM quay.io/wtsicgp/dockstore-cgpwxs:3.0.1 as builder USER root -RUN apt-get update -RUN apt-get install -qy --no-install-recommends lsb-release +RUN bash -c 'apt-get update -yq >& this.log || (cat this.log 1>&2 && exit 1)' +RUN bash -c 'apt-get install -qy --no-install-recommends lsb-release >& this.log || (cat this.log 1>&2 && exit 1)' RUN echo "deb http://cran.rstudio.com/bin/linux/ubuntu `lsb_release -cs`/" >> /etc/apt/sources.list RUN gpg --keyserver keyserver.ubuntu.com --recv-key E084DAB9 RUN gpg -a --export E084DAB9 | apt-key add - -RUN apt-get update -RUN apt-get install -qy --no-install-recommends\ +RUN bash -c 'apt-get update -yq >& this.log || (cat this.log 1>&2 && exit 1)' +RUN bash -c 'apt-get install -qy --no-install-recommends\ locales\ libcurl4-openssl-dev\ libssl-dev\ @@ -28,10 +28,11 @@ RUN apt-get install -qy --no-install-recommends\ r-base\ r-base-dev\ libblas-dev\ - libbz2-dev + libbz2-dev\ + >& this.log || (cat this.log 1>&2 && exit 1)' -RUN locale-gen en_US.UTF-8 -RUN update-locale LANG=en_US.UTF-8 +RUN bash -c 'locale-gen en_US.UTF-8 >& this.log || (cat this.log 1>&2 && exit 1)' +RUN bash -c 'update-locale LANG=en_US.UTF-8 >& this.log || (cat this.log 1>&2 && exit 1)' ENV OPT /opt/wtsi-cgp ENV PATH $OPT/bin:$OPT/biobambam2/bin:$PATH @@ -54,17 +55,17 @@ MAINTAINER keiranmraine@gmail.com LABEL vendor="Cancer Genome Project, Wellcome Trust Sanger Institute" LABEL uk.ac.sanger.cgp.description="CGP WGS pipeline for dockstore.org" -LABEL uk.ac.sanger.cgp.version="2.0.0-rc1" +LABEL uk.ac.sanger.cgp.version="2.0.0" -RUN apt-get update -RUN apt-get install -qy --no-install-recommends lsb-release +RUN bash -c 'apt-get update -yq >& this.log || (cat this.log 1>&2 && exit 1)' +RUN bash -c 'apt-get install -qy --no-install-recommends lsb-release >& this.log || (cat this.log 1>&2 && exit 1)' RUN echo "deb http://cran.rstudio.com/bin/linux/ubuntu `lsb_release -cs`/" >> /etc/apt/sources.list RUN gpg --keyserver keyserver.ubuntu.com --recv-key E084DAB9 RUN gpg -a --export E084DAB9 | apt-key add - -RUN apt-get -yq update -RUN apt-get install -yq --no-install-recommends\ +RUN bash -c 'apt-get update -yq >& this.log || (cat this.log 1>&2 && exit 1)' +RUN bash -c 'apt-get install -yq --no-install-recommends\ apt-transport-https\ locales\ curl\ @@ -81,10 +82,11 @@ RUN apt-get install -yq --no-install-recommends\ r-base\ exonerate\ libboost-iostreams-dev\ - p11-kit + p11-kit\ + >& this.log || (cat this.log 1>&2 && exit 1)' -RUN locale-gen en_US.UTF-8 -RUN update-locale LANG=en_US.UTF-8 +RUN bash -c 'locale-gen en_US.UTF-8 >& this.log || (cat this.log 1>&2 && exit 1)' +RUN bash -c 'update-locale LANG=en_US.UTF-8 >& this.log || (cat this.log 1>&2 && exit 1)' ENV OPT /opt/wtsi-cgp ENV PATH $OPT/bin:$OPT/biobambam2/bin:$PATH diff --git a/cwls/cgpwgs.cwl b/cwls/cgpwgs.cwl index df410dc..a8378f3 100644 --- a/cwls/cgpwgs.cwl +++ b/cwls/cgpwgs.cwl @@ -25,7 +25,7 @@ doc: | requirements: - class: DockerRequirement - dockerPull: "quay.io/wtsicgp/dockstore-cgpwgs:2.0.0-rc1" + dockerPull: "quay.io/wtsicgp/dockstore-cgpwgs:2.0.0" hints: - class: ResourceRequirement From 3f0c3e93cec5ba0dc10bffff088d03319e69d98a Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Sun, 18 Mar 2018 10:52:27 +0000 Subject: [PATCH 31/57] Changes to support full BB install --- Dockerfile | 3 ++- build/opt-build.sh | 3 ++- build/rlib-build.R | 17 +++++++++++------ 3 files changed, 15 insertions(+), 8 deletions(-) diff --git a/Dockerfile b/Dockerfile index f9f7d2b..e14d2ce 100644 --- a/Dockerfile +++ b/Dockerfile @@ -44,7 +44,8 @@ ENV R_LIBS $OPT/R-lib ENV R_LIBS_USER $R_LIBS COPY build/rlib-build.R build/ -RUN mkdir -p $R_LIBS_USER && Rscript build/rlib-build.R $R_LIBS_USER +RUN mkdir -p $R_LIBS_USER +RUN Rscript build/rlib-build.R $R_LIBS_USER 2>&1 | grep '^\*' COPY build/opt-build.sh build/ RUN bash build/opt-build.sh $OPT diff --git a/build/opt-build.sh b/build/opt-build.sh index b00a3b9..e5c26fb 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -28,7 +28,8 @@ SOURCE_BLAT="http://users.soe.ucsc.edu/~kent/src/blatSrc35.zip" SRC_FASTA36="https://github.com/wrpearson/fasta36/archive/v36.3.8d_13Apr16.tar.gz" ### cgpBattenberg -VER_CGPBB="v3.2.2" +#VER_CGPBB="v3.2.2" +VER_CGPBB="feature/docsAndRUpdates" if [ "$#" -lt "1" ] ; then diff --git a/build/rlib-build.R b/build/rlib-build.R index fd8b0e8..dfea7b7 100644 --- a/build/rlib-build.R +++ b/build/rlib-build.R @@ -9,16 +9,21 @@ source("http://bioconductor.org/biocLite.R") ipak <- function(pkg){ new.pkg <- pkg[!(pkg %in% installed.packages()[, "Package"])] if (length(new.pkg)) - biocLite(new.pkg, ask=FALSE, lib=instLib) + biocLite(new.pkg, ask=FALSE, lib=instLib, lib.loc=instLib) sapply(pkg, library, character.only = TRUE) } -# usage +tmp <- c("devtools") +ipak(tmp) +library(devtools) +options(download.file.method = "auto") + +# ASCAT and BRASS biocPackages <- c("data.table", "gam", "VGAM", "stringr", "poweRlaw", "zlibbioc", "RColorBrewer") ipak(biocPackages) -# use custom fix for copynumber library -install.packages("devtools", lib=instLib) -library(devtools) -options(download.file.method = "auto") install_github("sb43/copynumber", ref="f1688edc154f1a0e3aacf7781090afe02882f623") + +# add for BB +biocPackages <- c("stringi", "readr", "doParallel", "ggplot2", "gridExtra", "gtools") +ipak(biocPackages) From 0bf4f09768c9a82a768fe62382b29bcf35a944d3 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Sun, 18 Mar 2018 16:13:36 +0000 Subject: [PATCH 32/57] Handled misnaming of verifyBamID --- build/opt-build.sh | 8 +++++--- 1 file changed, 5 insertions(+), 3 deletions(-) diff --git a/build/opt-build.sh b/build/opt-build.sh index e5c26fb..8901286 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -13,7 +13,7 @@ VER_ALLELECOUNT="v4.0.0" ### cgpNgsQc VER_CGPNGSQC="v1.5.0" -VER_VERIFYBAM="1.1.3" +BIN_VERIFYBAMID='https://github.com/statgen/verifyBamID/releases/download/v1.1.3/verifyBamID' ### ascatNgs VER_ASCATNGS="v4.2.0" @@ -96,8 +96,10 @@ fi ### cgpNgsQc if [ ! -e $SETUP_DIR/cgpNgsQc.success ]; then - curl -sSL https://github.com/statgen/verifyBamID/releases/download/v${VER_VERIFYBAM}/verifyBamID.${VER_VERIFYBAM} > $OPT/bin/verifyBamId - chmod +x $OPT/bin/verifyBamId + curl -sSL $BIN_VERIFYBAMID > $OPT/bin/verifyBamID + chmod +x $OPT/bin/verifyBamID + # link to Id to handle misuse in cgpNgsQc + ln -s $OPT/bin/verifyBamID $OPT/bin/verifyBamId curl -sSL --retry 10 https://github.com/cancerit/cgpNgsQc/archive/${VER_CGPNGSQC}.tar.gz > distro.tar.gz rm -rf distro/* From 1c42bafaca62b8764f8e6467a70d7e35476b23c8 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Sun, 18 Mar 2018 16:14:00 +0000 Subject: [PATCH 33/57] Fix checking of alleleCounter and method of exec for docker tests --- .travis.yml | 44 ++++++++++++++++++++++---------------------- 1 file changed, 22 insertions(+), 22 deletions(-) diff --git a/.travis.yml b/.travis.yml index 6fd5d4b..bdfef3d 100644 --- a/.travis.yml +++ b/.travis.yml @@ -20,28 +20,28 @@ script: - docker build -t dockstore-cgpwgs . - docker images | grep -c dockstore-cgpwgs - echo 'Verify program from each inherited package is found (dockstore-cgpbigwig)' - - docker run -ti --rm dockstore-cgpwgs bwjoin --version + - docker run -t --rm dockstore-cgpwgs bwjoin --version - echo 'Verify program from each inherited package is found (dockstore-cgpmap)' - - docker run -ti --rm dockstore-cgpwgs ds-cgpmap.pl -h - - docker run -ti --rm dockstore-cgpwgs bwa_mem.pl -version - - docker run -ti --rm dockstore-cgpwgs bammarkduplicates2 --version - - docker run -ti --rm dockstore-cgpwgs samtools --version - - docker run -ti --rm dockstore-cgpwgs bash -c 'bwa 2>&1 | grep Version' + - docker run -t --rm dockstore-cgpwgs ds-cgpmap.pl -h + - docker run -t --rm dockstore-cgpwgs bwa_mem.pl -version + - docker run -t --rm dockstore-cgpwgs bammarkduplicates2 --version + - docker run -t --rm dockstore-cgpwgs samtools --version + - docker run -t --rm dockstore-cgpwgs bash -c 'bwa 2>&1 | grep Version' - echo 'Verify program from each inherited package is found (dockstore-cgpwxs)' - - docker run -ti --rm dockstore-cgpwgs ds-cgpwxs.pl -h - - docker run -ti --rm dockstore-cgpwgs cgpVCFSplit.pl -version - - docker run -ti --rm dockstore-cgpwgs vcftools --version - - docker run -ti --rm dockstore-cgpwgs bedtools --version - - docker run -ti --rm dockstore-cgpwgs bash -c 'pindel 2>&1 | grep Welcome' - - docker run -ti --rm dockstore-cgpwgs pindel.pl -version - - docker run -ti --rm dockstore-cgpwgs caveman --version - - docker run -ti --rm dockstore-cgpwgs caveman.pl -version - - docker run -ti --rm dockstore-cgpwgs cgpFlagCaVEMan.pl -version - - docker run -ti --rm dockstore-cgpwgs AnnotateVcf.pl -version + - docker run -t --rm dockstore-cgpwgs ds-cgpwxs.pl -h + - docker run -t --rm dockstore-cgpwgs cgpVCFSplit.pl -version + - docker run -t --rm dockstore-cgpwgs vcftools --version + - docker run -t --rm dockstore-cgpwgs bedtools --version + - docker run -t --rm dockstore-cgpwgs bash -c 'pindel 2>&1 | grep Welcome' + - docker run -t --rm dockstore-cgpwgs pindel.pl -version + - docker run -t --rm dockstore-cgpwgs caveman --version + - docker run -t --rm dockstore-cgpwgs caveman.pl -version + - docker run -t --rm dockstore-cgpwgs cgpFlagCaVEMan.pl -version + - docker run -t --rm dockstore-cgpwgs AnnotateVcf.pl -version - echo 'Verify a program from each new package is found (dockstore-cgpwgs)' - - docker run -ti --rm dockstore-cgpwgs ds-cgpwgs.pl -h - - docker run -ti --rm dockstore-cgpwgs bash -c 'verifyBamId 2>&1 | grep verifyBamID' - - docker run -ti --rm dockstore-cgpwgs alleleCount --version - - docker run -ti --rm dockstore-cgpwgs brass.pl -version - - docker run -ti --rm dockstore-cgpwgs ascat.pl -version - - docker run -ti --rm dockstore-cgpwgs battenberg.pl -version + - docker run -t --rm dockstore-cgpwgs ds-cgpwgs.pl -h + - docker run -t --rm dockstore-cgpwgs bash -c 'verifyBamID 2>&1 | grep verifyBamID' + - docker run -t --rm dockstore-cgpwgs alleleCounter --version + - docker run -t --rm dockstore-cgpwgs brass.pl -version + - docker run -t --rm dockstore-cgpwgs ascat.pl -version + - docker run -t --rm dockstore-cgpwgs battenberg.pl -version From 696ab9ce13cdda65133224bb1e21471e6162888c Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Mon, 19 Mar 2018 13:14:59 +0000 Subject: [PATCH 34/57] Put updated copynumber lib last in install --- build/rlib-build.R | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/build/rlib-build.R b/build/rlib-build.R index dfea7b7..c6655a5 100644 --- a/build/rlib-build.R +++ b/build/rlib-build.R @@ -22,8 +22,8 @@ options(download.file.method = "auto") biocPackages <- c("data.table", "gam", "VGAM", "stringr", "poweRlaw", "zlibbioc", "RColorBrewer") ipak(biocPackages) -install_github("sb43/copynumber", ref="f1688edc154f1a0e3aacf7781090afe02882f623") - # add for BB biocPackages <- c("stringi", "readr", "doParallel", "ggplot2", "gridExtra", "gtools") ipak(biocPackages) + +install_github("sb43/copynumber", ref="f1688edc154f1a0e3aacf7781090afe02882f623") From 96239fdec8107ee2603df2787d62388ccbe2d070 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 20 Mar 2018 15:04:12 +0000 Subject: [PATCH 35/57] Additional params, new librarys for updated R and minor changes to flow optimisation --- Dockerfile | 3 +++ build/opt-build.sh | 4 ++-- build/rlib-build.R | 2 +- cwls/cgpwgs.cwl | 23 ++++++++--------------- examples/cgpwgs/bam_bai.json | 4 ++++ examples/cgpwgs/bam_bai_pu_pl.json | 4 ++++ scripts/analysisWGS.sh | 16 ++++++++++------ scripts/ds-cgpwgs.pl | 11 +++++++---- 8 files changed, 39 insertions(+), 28 deletions(-) diff --git a/Dockerfile b/Dockerfile index e14d2ce..31bc5ce 100644 --- a/Dockerfile +++ b/Dockerfile @@ -14,6 +14,8 @@ RUN bash -c 'apt-get install -qy --no-install-recommends\ locales\ libcurl4-openssl-dev\ libssl-dev\ + libssh2-1-dev\ + libxml2-dev\ g++\ make\ gcc\ @@ -72,6 +74,7 @@ RUN bash -c 'apt-get install -yq --no-install-recommends\ curl\ ca-certificates\ libperlio-gzip-perl\ + libssh2-1\ bzip2\ psmisc\ time\ diff --git a/build/opt-build.sh b/build/opt-build.sh index 8901286..3e4a3a2 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -12,7 +12,7 @@ set -u VER_ALLELECOUNT="v4.0.0" ### cgpNgsQc -VER_CGPNGSQC="v1.5.0" +VER_CGPNGSQC="hotfix/v1.5.1" BIN_VERIFYBAMID='https://github.com/statgen/verifyBamID/releases/download/v1.1.3/verifyBamID' ### ascatNgs @@ -23,7 +23,7 @@ SRC_ASCAT="https://raw.githubusercontent.com/Crick-CancerGenomics/ascat/v2.5.1/A VER_GRASS="v2.1.0" ### BRASS -VER_BRASS="v6.1.0" +VER_BRASS="hotfix/v6.1.1" SOURCE_BLAT="http://users.soe.ucsc.edu/~kent/src/blatSrc35.zip" SRC_FASTA36="https://github.com/wrpearson/fasta36/archive/v36.3.8d_13Apr16.tar.gz" diff --git a/build/rlib-build.R b/build/rlib-build.R index c6655a5..9acee3d 100644 --- a/build/rlib-build.R +++ b/build/rlib-build.R @@ -19,7 +19,7 @@ library(devtools) options(download.file.method = "auto") # ASCAT and BRASS -biocPackages <- c("data.table", "gam", "VGAM", "stringr", "poweRlaw", "zlibbioc", "RColorBrewer") +biocPackages <- c("data.table", "mgcv", "gam", "VGAM", "stringr", "poweRlaw", "zlibbioc", "RColorBrewer") ipak(biocPackages) # add for BB diff --git a/cwls/cgpwgs.cwl b/cwls/cgpwgs.cwl index a8378f3..b09531a 100644 --- a/cwls/cgpwgs.cwl +++ b/cwls/cgpwgs.cwl @@ -39,7 +39,6 @@ inputs: doc: "The core reference (fa, fai, dict) as tar.gz" inputBinding: prefix: -reference - position: 1 separate: true annot: @@ -47,7 +46,6 @@ inputs: doc: "The VAGrENT cache files" inputBinding: prefix: -annot - position: 2 separate: true snv_indel: @@ -55,7 +53,6 @@ inputs: doc: "Supporting files for SNV and INDEL analysis" inputBinding: prefix: -snv_indel - position: 3 separate: true cnv_sv: @@ -63,7 +60,6 @@ inputs: doc: "Supporting files for CNV and SV analysis" inputBinding: prefix: -cnv_sv - position: 4 separate: true subcl: @@ -71,7 +67,13 @@ inputs: doc: "Supporting files for allele counts used by Battenberg Subclonal CNV analysis" inputBinding: prefix: -subcl - position: 5 + separate: true + + qcset: + type: File + doc: "Supporting files for QC tools" + inputBinding: + prefix: -qcset separate: true tumour: @@ -82,7 +84,6 @@ inputs: doc: "Tumour BAM or CRAM file" inputBinding: prefix: -tumour - position: 6 separate: true tumourIdx: @@ -90,7 +91,6 @@ inputs: doc: "Tumour alignment file index [bai|csi|crai]" inputBinding: prefix: -tidx - position: 7 separate: true normal: @@ -101,7 +101,6 @@ inputs: doc: "Normal BAM or CRAM file" inputBinding: prefix: -normal - position: 8 separate: true normalIdx: @@ -109,7 +108,6 @@ inputs: doc: "Normal alignment file index" inputBinding: prefix: -nidx - position: 9 separate: true exclude: @@ -117,7 +115,6 @@ inputs: doc: "Contigs to block during indel analysis" inputBinding: prefix: -exclude - position: 10 separate: true shellQuote: true @@ -127,7 +124,6 @@ inputs: default: '' inputBinding: prefix: -species - position: 11 separate: true shellQuote: true @@ -137,7 +133,6 @@ inputs: default: '' inputBinding: prefix: -assembly - position: 12 separate: true shellQuote: true @@ -146,7 +141,6 @@ inputs: doc: "Skip Battenberg allele counts" inputBinding: prefix: -skipbb - position: 13 separate: true cavereads: @@ -155,7 +149,6 @@ inputs: default: 350000 inputBinding: prefix: -cavereads - position: 14 separate: true purity: @@ -169,7 +162,7 @@ inputs: type: float? doc: "Set the ploidy (psi) for ascat when default solution needs additional guidance. If set purity is also required." inputBinding: - prefix: -pl + prefix: -pi separate: true diff --git a/examples/cgpwgs/bam_bai.json b/examples/cgpwgs/bam_bai.json index d6db44b..52c8a67 100644 --- a/examples/cgpwgs/bam_bai.json +++ b/examples/cgpwgs/bam_bai.json @@ -19,6 +19,10 @@ "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/SUBCL_ref_GRCh37d5.tar.gz", "class": "File" }, + "qcset": { + "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/qcGenotype_GRCh37d5.tar.gz", + "class": "File" + }, "tumour": { "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/12x/COLO-829.bam", "class": "File" diff --git a/examples/cgpwgs/bam_bai_pu_pl.json b/examples/cgpwgs/bam_bai_pu_pl.json index 5bb7cb6..61810c6 100644 --- a/examples/cgpwgs/bam_bai_pu_pl.json +++ b/examples/cgpwgs/bam_bai_pu_pl.json @@ -19,6 +19,10 @@ "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/SUBCL_ref_GRCh37d5.tar.gz", "class": "File" }, + "qcset": { + "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/qcGenotype_GRCh37d5.tar.gz", + "class": "File" + }, "tumour": { "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/12x/COLO-829.bam", "class": "File" diff --git a/scripts/analysisWGS.sh b/scripts/analysisWGS.sh index 46b5202..945493e 100755 --- a/scripts/analysisWGS.sh +++ b/scripts/analysisWGS.sh @@ -114,14 +114,14 @@ ln -fs $BAM_WT $BAM_WT_TMP ln -fs $BAM_WT.bas $BAM_WT_TMP.bas ln -fs $IDX_WT $IDX_WT_TMP -ASCAT_ADD_ARGS = '' +ASCAT_ADD_ARGS='' # ASCAT_PURITY set if [ ! -z ${ASCAT_PURITY+x} ]; then ASCAT_ADD_ARGS="$ASCAT_ADD_ARGS -pu $ASCAT_PURITY" fi # ASCAT_PLOIDY set if [ ! -z ${ASCAT_PLOIDY+x} ]; then - ASCAT_ADD_ARGS="$ASCAT_ADD_ARGS -pl $ASCAT_PLOIDY" + ASCAT_ADD_ARGS="$ASCAT_ADD_ARGS -pi $ASCAT_PLOIDY" fi ## Make fake copynumber so we can run early steps of caveman @@ -184,14 +184,17 @@ do_parallel[geno]="compareBamGenotypes.pl \ -o $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/genotyped \ -nb $BAM_WT_TMP \ -j $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/genotyped/result.json \ - -tb $BAM_MT_TMP" + -tb $BAM_MT_TMP \ + -s $REF_BASE/general.tsv \ + -g $REF_BASE/gender.tsv" echo -e "\t[Parallel block 1] VerifyBam Normal added..." do_parallel[verify_WT]="verifyBamHomChk.pl -d 25 \ -o $OUTPUT_DIR/${PROTOCOL}_${NAME_WT}/contamination \ -b $BAM_WT_TMP \ -t $CPU \ - -j $OUTPUT_DIR/${PROTOCOL}_${NAME_WT}/contamination/result.json" + -j $OUTPUT_DIR/${PROTOCOL}_${NAME_WT}/contamination/result.json \ + -s $REF_BASE/verifyBamID_snps.vcf.gz" echo "Starting Parallel block 1: `date`" run_parallel do_parallel @@ -317,7 +320,7 @@ declare -A do_parallel echo -e "\nSetting up Parallel block 4" echo -e "\t[Parallel block 4] cgpPindel added..." -do_parallel[cgpPindel]="nice -n 10 pindel.pl \ +do_parallel[cgpPindel]="pindel.pl \ -o $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/pindel \ -r $REF_BASE/genome.fa \ -t $BAM_MT_TMP \ @@ -438,7 +441,8 @@ do_parallel[verify_MT]="verifyBamHomChk.pl -d 25 \ -b $BAM_MT_TMP \ -t $CPU \ -a $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/ascat/${NAME_MT}.copynumber.caveman.csv \ - -j $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}/contamination/result.json" + -j $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}/contamination/result.json \ + -s $REF_BASE/verifyBamID_snps.vcf.gz" echo "Starting Parallel block 6: `date`" run_parallel do_parallel diff --git a/scripts/ds-cgpwgs.pl b/scripts/ds-cgpwgs.pl index 8d2e2c1..72b7973 100644 --- a/scripts/ds-cgpwgs.pl +++ b/scripts/ds-cgpwgs.pl @@ -17,7 +17,7 @@ 'sp' => undef, 'as' => undef, 'pu' => undef, - 'pl' => undef, + 'pi' => undef, ); GetOptions( 'h|help' => \$opts{'h'}, @@ -27,6 +27,7 @@ 'si|snv_indel=s' => \$opts{'si'}, 'cs|cnv_sv=s' => \$opts{'cs'}, 'sc|subcl=s' => \$opts{'sc'}, + 'qc|qcset=s' => \$opts{'qc'}, 't|tumour=s' => \$opts{'t'}, 'tidx=s' => \$opts{'tidx'}, 'n|normal=s' => \$opts{'n'}, @@ -39,13 +40,13 @@ 'c|cores:i' => \$opts{'c'}, 'o|outdir:s' => \$opts{'o'}, 'pu|purity:f' => \$opts{'pu'}, - 'pl|ploidy:f' => \$opts{'pl'}, + 'pi|ploidy:f' => \$opts{'pi'}, ) or pod2usage(2); pod2usage(-verbose => 1, -exitval => 0) if(defined $opts{'h'}); pod2usage(-verbose => 2, -exitval => 0) if(defined $opts{'m'}); -if((defined($opts{'pu'}) && !defined($opts{'pl'})) || (!defined($opts{'pu'}) && defined($opts{'pl'}))) { +if((defined($opts{'pu'}) && !defined($opts{'pi'})) || (!defined($opts{'pu'}) && defined($opts{'pi'}))) { pod2usage(-msg => "\nERROR: If one of purity/ploidy are defined, both should be defined.\n", -verbose => 1, -output => \*STDERR); } @@ -67,6 +68,7 @@ if($opts{'r'} eq $opts{'a'} && $opts{'r'} eq $opts{'si'} && $opts{'r'} eq $opts{'cs'} + && $opts{'r'} eq $opts{'qc'} && (exists $opts{'sb'} || $opts{'r'} eq $opts{'sc'}) && -d $opts{'r'}) { $ref_area = $opts{'r'}; @@ -77,6 +79,7 @@ ref_unpack($ref_area, $opts{'a'}); ref_unpack($ref_area, $opts{'si'}); ref_unpack($ref_area, $opts{'cs'}); + ref_unpack($ref_area, $opts{'qc'}); ref_unpack($ref_area, $opts{'sc'}) if(! exists $opts{'sb'}); } @@ -105,7 +108,7 @@ printf $FH "CAVESPLIT='%s'\n", $opts{'cr'}; printf $FH "SNVFLAG='%s'\n", $ini; printf $FH "CPU=%d\n", $opts{'c'} if(defined $opts{'c'}); -printf $FH "ASCAT_PLOIDY=%.5f\n", $opts{'pl'} if(defined $opts{'pl'}); +printf $FH "ASCAT_PLOIDY=%.5f\n", $opts{'pi'} if(defined $opts{'pi'}); printf $FH "ASCAT_PURITY=%.5f\n", $opts{'pu'} if(defined $opts{'pu'}); printf $FH "CLEAN_REF=1\n" if($ref_unpack); # Options to disable algorithms From 0f8a05b3740adf813270be2c8ff1a015877d5ba6 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 20 Mar 2018 16:14:04 +0000 Subject: [PATCH 36/57] Docs [ci skip] --- README.md | 103 ++++++++++++++++++++++++++++++++++++++++++++++++------ 1 file changed, 93 insertions(+), 10 deletions(-) diff --git a/README.md b/README.md index 0dbfb1a..5e362c8 100644 --- a/README.md +++ b/README.md @@ -16,8 +16,13 @@ the [Dockstore.org](https://dockstore.org/) framework. - [Usage](#usage) - [Usable Cores](#usable-cores) +- [Other uses](#other-uses) + - [Native docker](#native-docker) + - [Singularity](#singularity) +- [Verifying your deployment](#verifying-your-deployment) - [Example data](#example-data) - [Diagram of internals](#diagram-of-internals) +- [Development environment](#development-environment) - [Release process](#release-process) - [LICENCE](#licence) @@ -28,6 +33,8 @@ the [Dockstore.org](https://dockstore.org/) framework. This is intended to be run using the Dockstore.org framework under docker but can be executed as a normal docker container (or imported into singularity. +See the [dockstore execution method here][dockstore-cgpwgs]. + You should see the usage for the `ds-cgpwgs.pl` script for all parameters (or the `cwl` definition). Required input files are @@ -40,9 +47,23 @@ Required input files are 1. VAGrENT (annotation) reference archive (e.g. [VAGrENT_ref_GRCh37d5_ensembl_75.tar.gz][ftp-ref]) 1. Subclonal reference archive ([SUBCL_ref_GRCh37d5.tar.gz][ftp-ref]) * Only needed if `skipbb` is `false` +1. QC reference archive (e.g. [qcGenotype_GRCh37d5.tar.gz][ftp-ref]) Inputs 1&2 are expected to have been mapped using [dockstore-cgpmap][dockstore-cgpmap] +Please check the Wiki then raise an issue if you require additional information on how to generate +your own reference files. Much of this information is available on the individual algorithm wiki +pages (or the subsequently linked protocols papers). + +* Specific to dockstore-cgpwgs: + * [BRASS][brass-wiki] + * [ascatNgs][ascatngs-wiki] +* Inherited from dockstore-cgpwxs: + * [cgpCaVEManWrapper][caveman-wiki] + * [cgpPindel][cgppindel-wiki] + * [VAGrENT][vagrent-wiki] + + ### Usable Cores When running outside of a docker container you can set the number of CPUs via: @@ -52,19 +73,65 @@ When running outside of a docker container you can set the number of CPUs via: If not set detects available cores on system. -## Example data +## Other uses -The data linked in the 'examples' area is from the cell line COLO-829. +### Native docker -Please check the Wiki then raise an issue if you require additional information on how to generate -your own reference files. Much of this information is available on the individual algorithm wiki -pages (or the subsequently linked protocols papers). +All of the tools installed as part of [dockstore-cgpmap][dockstore-cgpmap] and +[dockstore-cgpwxs][dockstore-cgpwxs] and the above packages are available for direct use. + +``` +export CGPWGS_VER=X.X.X +docker pull quay.io/wtsicgp/dockstore-cgpwgs:$CGPWGS_VER +docker --rm -ti [--volume ...] quay.io/wtsicgp/dockstore-cgpwgs:$CGPWGS_VER bash +``` + +### Singularity + +The resulting docker container has been tested with Singularity. The command to exec is: + +``` +ds-cgpwgs.pl -h +``` + +Expected use would be along the lines of: + +``` +export CGPWGS_VER=X.X.X +singularity pull docker://quay.io/wtsicgp/dockstore-cgpwgs:$CGPWGS_VER + +singularity exec\ + --workdir /.../workspace \ + --home /.../workspace:/home \ + --bind /.../ref/human:/var/spool/ref:ro \ + --bind /.../example_data/cgpwgs:/var/spool/data:ro \ + dockstore-cgpwgs-${CGPWGS_VER}.simg \ + ds-cgpwgs.pl \ + -reference /var/spool/ref/core_ref_GRCh37d5.tar.gz \ + -annot /var/spool/ref/VAGrENT_ref_GRCh37d5_ensembl_75.tar.gz \ + -snv_indel /var/spool/ref/SNV_INDEL_ref_GRCh37d5-fragment.tar.gz \ + -tumour /var/spool/data/COLO-829_ex.cram \ + ... +``` -* [BRASS][brass-wiki] -* [cgpCaVEManWrapper][caveman-wiki] -* [cgpPindel][cgppindel-wiki] -* [ascatNgs][ascatngs-wiki] -* [VAGrENT][vagrent-wiki] +For a system automatically attaching _all local mount points_ (not default singularity behaviour) +you need not specify any `exec` params (workdir, home, bind) but you should specify the `-outdir` +option for `ds-cgpwgs.pl` to prevent data being written to your home directory. + +By default results are written to the home directory of the container so ensure you bind +a large volume and set the `-home` variable. As indicated above the location can be overridden +via the options of `ds-cgpwgs.pl` + +## Verifying your deployment + +The `examples/` tree contains test json files populated with data that can be used to verify the +tool. More details can be found [here](examples/README.md). + +The `expected/` tree contains the expected output for each tool. More details can be found [here](expected/README.md). + +## Example data + +The data linked in the 'examples' area is from the cell line COLO-829. ## Diagram of internals @@ -73,11 +140,26 @@ handled by a Dockstore run. ![Internal flow of docker image](images/dockstore-cgpwgs.png) +## Development environment + +This project uses git pre-commit hooks. Please enable them to prevent inappropriate large files +being included. Any pull request found not to have adhered to this will be rejected and the branch +will need to be manually cleaned to keep the repo size down. + +Activate the hooks with + +``` +git config core.hooksPath git-hooks +``` + ## Release process This project is maintained using HubFlow. 1. Make appropriate changes +1. Build image locally +1. Run all example inputs and verify any changes are acceptable +1. Update `expected/` tree with output of last step 1. Bump version in `Dockerfile` and `Dockstore.cwl` 1. Push changes 1. Check state on Travis @@ -143,5 +225,6 @@ identical to a statement that reads ‘Copyright (c) 2005, 2006, 2007, 2008, [dockstore-cgpwgs]: https://dockstore.org/containers/quay.io/wtsicgp/dockstore-cgpwgs +[dockstore-cgpwxs]: https://dockstore.org/containers/quay.io/wtsicgp/dockstore-cgpwxs [dockstore-cgpmap]: https://dockstore.org/containers/quay.io/wtsicgp/dockstore-cgpmap [dockstore-get-started]: https://dockstore.org/docs/getting-started-with-dockstore From 197c21eeec40b1e115a5b13c41fdff89ee1d69be Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 20 Mar 2018 16:16:37 +0000 Subject: [PATCH 37/57] Ready for addition of expected results [ci skip] --- git-hooks/pre-commit | 67 ++++++++++++++++++++++++++++++++++++++++++++ 1 file changed, 67 insertions(+) create mode 100755 git-hooks/pre-commit diff --git a/git-hooks/pre-commit b/git-hooks/pre-commit new file mode 100755 index 0000000..08cf780 --- /dev/null +++ b/git-hooks/pre-commit @@ -0,0 +1,67 @@ +#!/bin/sh +# +# An example hook script to verify what is about to be committed. +# Called by "git commit" with no arguments. The hook should +# exit with non-zero status after issuing an appropriate message if +# it wants to stop the commit. +# +# To enable this hook, rename this file to "pre-commit". + +if git rev-parse --verify HEAD >/dev/null 2>&1 +then + against=HEAD +else + # Initial commit: diff against an empty tree object + against=4b825dc642cb6eb9a060e54bf8d69288fbee4904 +fi + +# If you want to allow non-ASCII filenames set this variable to true. +allownonascii=$(git config --bool hooks.allownonascii) + +# Redirect output to stderr. +exec 1>&2 + +# Cross platform projects tend to avoid non-ASCII filenames; prevent +# them from being added to the repository. We exploit the fact that the +# printable range starts at the space character and ends with tilde. +if [ "$allownonascii" != "true" ] && + # Note that the use of brackets around a tr range is ok here, (it's + # even required, for portability to Solaris 10's /usr/bin/tr), since + # the square bracket bytes happen to fall in the designated range. + test $(git diff --cached --name-only --diff-filter=A -z $against | + LC_ALL=C tr -d '[ -~]\0' | wc -c) != 0 +then + cat <<\EOF +Error: Attempt to add a non-ASCII file name. + +This can cause problems if you want to work with people on other platforms. + +To be portable it is advisable to rename the file. + +If you know what you are doing you can disable this check using: + + git config hooks.allownonascii true +EOF + exit 1 +fi + +## Now we want to warn that certain files should have size==0 +TO_MINIMISE=`find expected -type f \( -iname \*.bai -o -iname \*.crai -o -iname \*.tbi -o -iname \*.md5 -o -iname prob_arr -o -iname \*.flagged.muts.vcf.gz -o -iname \*.muts.ids.vcf.gz -o -iname \*.flagged.vcf.gz \) -size +1c` + +if [ ! -z "$TO_MINIMISE" ]; then + >&2 cat << EOF + +ERROR: The following files should be truncated to size of 0 before commit: + +$TO_MINIMISE + + To do this quickly run: + + find expected -type f \( -iname \*.bai -o -iname \*.crai -o -iname \*.tbi -o -iname \*.md5 -o -iname prob_arr -o -iname \*.flagged.muts.vcf.gz -o -iname \*.muts.ids.vcf.gz -o -iname \*.flagged.vcf.gz \) -size +1c -exec bash -c 'echo > {}' \; + +EOF + exit 1 +fi + +# If there are whitespace errors, print the offending file names and fail. +exec git diff-index --check --cached $against -- From c6d642bfa9de35208d5c7b22b005a2c058f89e82 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 21 Mar 2018 20:22:38 +0000 Subject: [PATCH 38/57] Update fasta package --- build/opt-build.sh | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/build/opt-build.sh b/build/opt-build.sh index 3e4a3a2..fe26733 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -25,7 +25,7 @@ VER_GRASS="v2.1.0" ### BRASS VER_BRASS="hotfix/v6.1.1" SOURCE_BLAT="http://users.soe.ucsc.edu/~kent/src/blatSrc35.zip" -SRC_FASTA36="https://github.com/wrpearson/fasta36/archive/v36.3.8d_13Apr16.tar.gz" +SRC_FASTA36="https://github.com/wrpearson/fasta36/archive/fasta-v36.3.8g.tar.gz" ### cgpBattenberg #VER_CGPBB="v3.2.2" From 6a611181642438b5fde0f7571ef0a75db984b1f5 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 21 Mar 2018 20:22:58 +0000 Subject: [PATCH 39/57] More robust building of Rlibs --- build/rlib-build.R | 18 ++++++++++++++---- 1 file changed, 14 insertions(+), 4 deletions(-) diff --git a/build/rlib-build.R b/build/rlib-build.R index 9acee3d..dddaab1 100644 --- a/build/rlib-build.R +++ b/build/rlib-build.R @@ -19,11 +19,21 @@ library(devtools) options(download.file.method = "auto") # ASCAT and BRASS -biocPackages <- c("data.table", "mgcv", "gam", "VGAM", "stringr", "poweRlaw", "zlibbioc", "RColorBrewer") -ipak(biocPackages) +ipak(c("data.table")) +ipak(c("mgcv")) +ipak(c("gam")) +ipak(c("VGAM")) +ipak(c("stringr")) +ipak(c("poweRlaw")) +ipak(c("zlibbioc")) +ipak(c("RColorBrewer")) # add for BB -biocPackages <- c("stringi", "readr", "doParallel", "ggplot2", "gridExtra", "gtools") -ipak(biocPackages) +ipak(c("stringi")) +ipak(c("readr")) +ipak(c("doParallel")) +ipak(c("ggplot2")) +ipak(c("gridExtra")) +ipak(c("gtools")) install_github("sb43/copynumber", ref="f1688edc154f1a0e3aacf7781090afe02882f623") From 7e43d10ba42fe1643a363d550e97fb75bedc5ef4 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Sun, 25 Mar 2018 15:39:49 +0100 Subject: [PATCH 40/57] Remove reference to bai as secondary file as index handed explicitly now --- cwls/cgpwgs.cwl | 2 -- 1 file changed, 2 deletions(-) diff --git a/cwls/cgpwgs.cwl b/cwls/cgpwgs.cwl index b09531a..4d73826 100644 --- a/cwls/cgpwgs.cwl +++ b/cwls/cgpwgs.cwl @@ -79,7 +79,6 @@ inputs: tumour: type: File secondaryFiles: - - .bai - .bas doc: "Tumour BAM or CRAM file" inputBinding: @@ -96,7 +95,6 @@ inputs: normal: type: File secondaryFiles: - - .bai - .bas doc: "Normal BAM or CRAM file" inputBinding: From fbc66330e9feca2dfcf3c88c8b8616eea6152654 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Sun, 25 Mar 2018 15:40:20 +0100 Subject: [PATCH 41/57] Naming error, content was correct --- scripts/ds-cgpwgs.pl | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/scripts/ds-cgpwgs.pl b/scripts/ds-cgpwgs.pl index 72b7973..f8f9ea7 100644 --- a/scripts/ds-cgpwgs.pl +++ b/scripts/ds-cgpwgs.pl @@ -159,7 +159,7 @@ sub add_species_flag_ini { my ($species, $ini_in) = @_; $species =~ s/ /_/g; $species = uc $species; - my $ini_out = $opts{'o'}.'/flag.vcf.config.WXS.ini'; + my $ini_out = $opts{'o'}.'/flag.vcf.config.WGS.ini'; open my $IN, '<', $ini_in; open my $OUT,'>',$ini_out; while(my $line = <$IN>) { From 17272dffceaf8b9f5f1b68d8370f05bc5f32addb Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Sun, 25 Mar 2018 15:41:15 +0100 Subject: [PATCH 42/57] New releases and more robust use of curl for single files --- build/opt-build.sh | 12 +++++------- 1 file changed, 5 insertions(+), 7 deletions(-) diff --git a/build/opt-build.sh b/build/opt-build.sh index fe26733..f656fd2 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -12,7 +12,7 @@ set -u VER_ALLELECOUNT="v4.0.0" ### cgpNgsQc -VER_CGPNGSQC="hotfix/v1.5.1" +VER_CGPNGSQC="v1.5.1" BIN_VERIFYBAMID='https://github.com/statgen/verifyBamID/releases/download/v1.1.3/verifyBamID' ### ascatNgs @@ -23,14 +23,12 @@ SRC_ASCAT="https://raw.githubusercontent.com/Crick-CancerGenomics/ascat/v2.5.1/A VER_GRASS="v2.1.0" ### BRASS -VER_BRASS="hotfix/v6.1.1" +VER_BRASS="v6.1.1" SOURCE_BLAT="http://users.soe.ucsc.edu/~kent/src/blatSrc35.zip" SRC_FASTA36="https://github.com/wrpearson/fasta36/archive/fasta-v36.3.8g.tar.gz" ### cgpBattenberg -#VER_CGPBB="v3.2.2" -VER_CGPBB="feature/docsAndRUpdates" - +VER_CGPBB="v3.3.0" if [ "$#" -lt "1" ] ; then echo "Please provide an installation path such as /opt/ICGC" @@ -96,7 +94,7 @@ fi ### cgpNgsQc if [ ! -e $SETUP_DIR/cgpNgsQc.success ]; then - curl -sSL $BIN_VERIFYBAMID > $OPT/bin/verifyBamID + curl --fail -sSL $BIN_VERIFYBAMID > $OPT/bin/verifyBamID chmod +x $OPT/bin/verifyBamID # link to Id to handle misuse in cgpNgsQc ln -s $OPT/bin/verifyBamID $OPT/bin/verifyBamId @@ -120,7 +118,7 @@ if [ ! -e $SETUP_DIR/ascatNgs.success ]; then cd distro/perl # add ascatSrc - curl -sSL $SRC_ASCAT > share/ascat/ascat.R + curl --fail -sSL $SRC_ASCAT > share/ascat/ascat.R cpanm --no-interactive --notest --mirror http://cpan.metacpan.org --notest -l $INST_PATH --installdeps . cpanm -v --no-interactive --mirror http://cpan.metacpan.org -l $INST_PATH . cd $SETUP_DIR From f890ec3a5fb47941bf3f4b5b5b17435c0e784695 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Sun, 25 Mar 2018 15:41:28 +0100 Subject: [PATCH 43/57] Detail the changes --- CHANGES.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGES.md b/CHANGES.md index 860555a..2a88c64 100644 --- a/CHANGES.md +++ b/CHANGES.md @@ -2,6 +2,7 @@ ## 2.0.0 +* Full support for BAM+CSI and CRAM+CRAI as inputs * Reorganisation to allow access of other tools or necessary bindings * See dockstore-cgpmap 3.0.0 - 3.0.2 (primarily adds mismatchQc and threads to some tools) * See dockstore-cgpwxs 3.0.0 - 3.0.1 (fragment based analysis) From 59d66d4d5a7c39266ec5ec84bbdd165cf3789ce1 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Sun, 25 Mar 2018 15:44:22 +0100 Subject: [PATCH 44/57] Minor change to license email/date --- README.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/README.md b/README.md index 5e362c8..6f1c7a4 100644 --- a/README.md +++ b/README.md @@ -171,9 +171,9 @@ This project is maintained using HubFlow. ## LICENCE ``` -Copyright (c) 2017 Genome Research Ltd. +Copyright (c) 2017-2018 Genome Research Ltd. -Author: Cancer Genome Project +Author: Cancer Genome Project This file is part of dockstore-cgpwgs. From 2d109c219fd53925cc68b9612ecc4639f56dcc0f Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 28 Mar 2018 11:36:34 +0100 Subject: [PATCH 45/57] Update versions for tabix query issues on GRCh38 --- build/opt-build.sh | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/build/opt-build.sh b/build/opt-build.sh index f656fd2..40f59c5 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -16,14 +16,14 @@ VER_CGPNGSQC="v1.5.1" BIN_VERIFYBAMID='https://github.com/statgen/verifyBamID/releases/download/v1.1.3/verifyBamID' ### ascatNgs -VER_ASCATNGS="v4.2.0" +VER_ASCATNGS="v4.2.1" SRC_ASCAT="https://raw.githubusercontent.com/Crick-CancerGenomics/ascat/v2.5.1/ASCAT/R/ascat.R" ### grass -VER_GRASS="v2.1.0" +VER_GRASS="v2.1.1" ### BRASS -VER_BRASS="v6.1.1" +VER_BRASS="v6.1.2" SOURCE_BLAT="http://users.soe.ucsc.edu/~kent/src/blatSrc35.zip" SRC_FASTA36="https://github.com/wrpearson/fasta36/archive/fasta-v36.3.8g.tar.gz" From 8e0b6f3f10afaa02d1f3426049bd4fb668ad2a45 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Thu, 29 Mar 2018 11:54:48 +0100 Subject: [PATCH 46/57] Limit max pindel threads to prevent memory explosions --- scripts/analysisWGS.sh | 7 ++++++- 1 file changed, 6 insertions(+), 1 deletion(-) diff --git a/scripts/analysisWGS.sh b/scripts/analysisWGS.sh index 945493e..47bc4ff 100755 --- a/scripts/analysisWGS.sh +++ b/scripts/analysisWGS.sh @@ -67,6 +67,11 @@ if [ -z ${CPU+x} ]; then CPU=`grep -c ^processor /proc/cpuinfo` fi +PINDEL_CPU=$CPU +if [ $PINDEL_CPU -gt 8 ]; + PINDEL_CPU=8 +fi + # create area which allows monitoring site to be started, not actively updated until after PRE-EXEC completes #cp -r /opt/wtsi-cgp/site $OUTPUT_DIR/site @@ -334,7 +339,7 @@ do_parallel[cgpPindel]="pindel.pl \ -sp '$SPECIES' \ -e $CONTIG_EXCLUDE \ -b $REF_BASE/pindel/HiDepth.bed.gz \ - -c $CPU \ + -c $PINDEL_CPU \ -sf $REF_BASE/pindel/softRules.lst" echo -e "\t[Parallel block 4] CaVEMan added..." From 434dafcefdaed1ae9f5754b0ac89943c9ad7d6ef Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 10 Apr 2018 13:16:19 +0100 Subject: [PATCH 47/57] Update base image --- Dockerfile | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/Dockerfile b/Dockerfile index 31bc5ce..6ffbb1d 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM quay.io/wtsicgp/dockstore-cgpwxs:3.0.1 as builder +FROM quay.io/wtsicgp/dockstore-cgpwxs:3.0.2 as builder USER root From ebb4847464772fe0dc68ef9fc422811e8842f509 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 11 Apr 2018 14:11:32 +0100 Subject: [PATCH 48/57] Example results cleaned to leave informative entries --- examples/cgpwgs/bam_bai.json | 8 +- examples/cgpwgs/bam_bai_pu_pl.json | 8 +- .../contamination/COLO-829-BL.depthRG | 13468 ++++++ .../contamination/COLO-829-BL.depthSM | 202 + .../contamination/COLO-829-BL.log | 1 + .../contamination/COLO-829-BL.selfRG | 68 + .../contamination/COLO-829-BL.selfSM | 2 + .../contamination/COLO-829-BL_snps.vcf | 35408 ++++++++++++++++ .../WGS_COLO-829-BL/contamination/result.json | 1 + .../contamination/COLO-829.depthRG | 20704 +++++++++ .../contamination/COLO-829.depthSM | 202 + .../WGS_COLO-829/contamination/COLO-829.log | 1 + .../contamination/COLO-829.selfRG | 104 + .../contamination/COLO-829.selfSM | 2 + .../contamination/COLO-829_snps.vcf | 20961 +++++++++ .../WGS_COLO-829/contamination/result.json | 1 + .../ascat/COLO-829.ASCATprofile.png | Bin 0 -> 42504 bytes .../ascat/COLO-829.ASPCF.png | Bin 0 -> 411586 bytes .../ascat/COLO-829.copynumber.baf.bw | 1 + .../ascat/COLO-829.copynumber.caveman.csv | 240 + .../ascat/COLO-829.copynumber.caveman.vcf.gz | Bin 0 -> 4572 bytes .../COLO-829.copynumber.caveman.vcf.gz.tbi | 1 + .../ascat/COLO-829.copynumber.logR.bw | 1 + .../ascat/COLO-829.copynumber.minorCn.bw | 1 + .../ascat/COLO-829.copynumber.rawCn.bw | 1 + .../ascat/COLO-829.copynumber.segBaf.bw | 1 + .../ascat/COLO-829.copynumber.segLogR.bw | 1 + .../ascat/COLO-829.copynumber.totalCn.bw | 1 + .../ascat/COLO-829.copynumber.txt.gz | 1 + .../ascat/COLO-829.germline.png | Bin 0 -> 212694 bytes .../ascat/COLO-829.rawprofile.png | Bin 0 -> 55454 bytes .../ascat/COLO-829.samplestatistics.txt | 7 + .../ascat/COLO-829.sunrise.png | Bin 0 -> 35675 bytes .../ascat/COLO-829.tumour.png | Bin 0 -> 359125 bytes .../brass/COLO-829-BL.brm.bam | 1 + .../brass/COLO-829-BL.brm.bam.bai | 1 + .../brass/COLO-829.brm.bam | 1 + .../brass/COLO-829.brm.bam.bai | 1 + .../COLO-829_vs_COLO-829-BL.annot.bedpe.gz | Bin 0 -> 1997 bytes ...COLO-829_vs_COLO-829-BL.annot.bedpe.gz.tbi | 1 + .../COLO-829_vs_COLO-829-BL.annot.vcf.gz | Bin 0 -> 3636 bytes .../COLO-829_vs_COLO-829-BL.annot.vcf.gz.tbi | 1 + ...LO-829_vs_COLO-829-BL.intermediates.tar.gz | 1 + .../COLO-829_vs_COLO-829-BL.ngscn.abs_cn.bw | 1 + .../COLO-829_vs_COLO-829-BL.annot.muts.vcf.gz | Bin 0 -> 9112 bytes ...O-829_vs_COLO-829-BL.annot.muts.vcf.gz.tbi | 1 + ...OLO-829_vs_COLO-829-BL.flagged.muts.vcf.gz | 1 + ...829_vs_COLO-829-BL.flagged.muts.vcf.gz.tbi | 1 + .../COLO-829_vs_COLO-829-BL.muts.ids.vcf.gz | 1 + ...OLO-829_vs_COLO-829-BL.muts.ids.vcf.gz.tbi | 1 + .../COLO-829_vs_COLO-829-BL.no_analysis.bed | 34944 +++++++++++++++ .../COLO-829_vs_COLO-829-BL.snps.ids.vcf.gz | Bin 0 -> 775266 bytes ...OLO-829_vs_COLO-829-BL.snps.ids.vcf.gz.tbi | 1 + .../caveman/alg_bean | 4 + .../caveman/caveman.cfg.ini | 14 + .../caveman/cov_arr | Bin 0 -> 454298 bytes .../caveman/prob_arr | 1 + .../caveman/splitList | 3 + .../genotyped/COLO-829-BL.full_gender.tsv | 5 + .../genotyped/COLO-829-BL.full_genotype.tsv | 93 + .../genotyped/COLO-829.full_gender.tsv | 5 + .../genotyped/COLO-829.full_genotype.tsv | 93 + .../COLO-829_vs_COLO-829-BL.genotype.txt | 10 + .../genotyped/result.json | 1 + .../COLO-829_vs_COLO-829-BL.annot.vcf.gz | Bin 0 -> 8670 bytes .../COLO-829_vs_COLO-829-BL.annot.vcf.gz.tbi | 1 + .../COLO-829_vs_COLO-829-BL.flagged.vcf.gz | 1 + ...COLO-829_vs_COLO-829-BL.flagged.vcf.gz.tbi | 1 + .../COLO-829_vs_COLO-829-BL.germline.bed.gz | Bin 0 -> 545 bytes ...OLO-829_vs_COLO-829-BL.germline.bed.gz.tbi | 1 + .../pindel/COLO-829_vs_COLO-829-BL_mt.bam | Bin 0 -> 35723 bytes .../pindel/COLO-829_vs_COLO-829-BL_mt.bam.bai | 1 + .../pindel/COLO-829_vs_COLO-829-BL_mt.bam.md5 | 1 + .../pindel/COLO-829_vs_COLO-829-BL_wt.bam | Bin 0 -> 30326 bytes .../pindel/COLO-829_vs_COLO-829-BL_wt.bam.bai | 1 + .../pindel/COLO-829_vs_COLO-829-BL_wt.bam.md5 | 1 + git-hooks/pre-commit | 4 +- 77 files changed, 126586 insertions(+), 10 deletions(-) create mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL.depthRG create mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL.depthSM create mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL.log create mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL.selfRG create mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL.selfSM create mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL_snps.vcf create mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/result.json create mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/COLO-829.depthRG create mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/COLO-829.depthSM create mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/COLO-829.log create mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/COLO-829.selfRG create mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/COLO-829.selfSM create mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/COLO-829_snps.vcf create mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/result.json create mode 100644 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mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/ascat/COLO-829.copynumber.segBaf.bw create mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/ascat/COLO-829.copynumber.segLogR.bw create mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/ascat/COLO-829.copynumber.totalCn.bw create mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/ascat/COLO-829.copynumber.txt.gz create mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/ascat/COLO-829.germline.png create mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/ascat/COLO-829.rawprofile.png create mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/ascat/COLO-829.samplestatistics.txt create mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/ascat/COLO-829.sunrise.png create mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/ascat/COLO-829.tumour.png create mode 100644 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expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_wt.bam.bai create mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_wt.bam.md5 diff --git a/examples/cgpwgs/bam_bai.json b/examples/cgpwgs/bam_bai.json index 52c8a67..6b9d5e2 100644 --- a/examples/cgpwgs/bam_bai.json +++ b/examples/cgpwgs/bam_bai.json @@ -24,19 +24,19 @@ "class": "File" }, "tumour": { - "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/12x/COLO-829.bam", + "path": "http://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/sampled/COLO-829.bam", "class": "File" }, "tumourIdx": { - "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/12x/COLO-829.bam.bai", + "path": "http://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/sampled/COLO-829.bam.bai", "class": "File" }, "normal": { - "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/12x/COLO-829-BL.bam", + "path": "http://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/sampled/COLO-829-BL.bam", "class": "File" }, "normalIdx": { - "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/12x/COLO-829-BL.bam.bai", + "path": "http://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/sampled/COLO-829-BL.bam.bai", "class": "File" }, "exclude": "1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,22,X,Y,MT,NC_007605,hs37d5,GL%", diff --git a/examples/cgpwgs/bam_bai_pu_pl.json b/examples/cgpwgs/bam_bai_pu_pl.json index 61810c6..d31ad27 100644 --- a/examples/cgpwgs/bam_bai_pu_pl.json +++ b/examples/cgpwgs/bam_bai_pu_pl.json @@ -24,19 +24,19 @@ "class": "File" }, "tumour": { - "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/12x/COLO-829.bam", + "path": "http://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/sampled/COLO-829.bam", "class": "File" }, "tumourIdx": { - "path": "ftp://ngs.sanger.ac.uk/production/cancer/dockstore/cgpwgs/12x/COLO-829.bam.bai", + "path": 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35283 21872 0.62 0.00000 9421.76 9421.76 NA NA NA NA NA NA NA NA NA NA +COLO-829-BL 2720 NA 35283 15939 0.45 0.00000 6965.54 6965.54 NA NA NA NA NA NA NA NA NA NA +COLO-829-BL 2722 NA 35283 18407 0.52 0.00000 8049.76 8049.76 NA NA NA NA NA NA NA NA NA NA +COLO-829-BL 2724 NA 35283 22054 0.63 0.00000 9468.13 9468.13 NA NA NA NA NA NA NA NA NA NA +COLO-829-BL 2727 NA 35283 21526 0.61 0.00000 9304.01 9304.01 NA NA NA NA NA NA NA NA NA NA +COLO-829-BL 2729 NA 35283 19194 0.54 0.00000 8278.21 8278.21 NA NA NA NA NA NA NA NA NA NA +COLO-829-BL 2731 NA 35283 21456 0.61 0.00000 9403.38 9403.38 NA NA NA NA NA NA NA NA NA NA +COLO-829-BL 2733 NA 35283 17864 0.51 0.00000 7830.53 7830.53 NA NA NA NA NA NA NA NA NA NA +COLO-829-BL 2735 NA 35283 21201 0.60 0.00000 9077.09 9077.09 NA NA NA NA NA NA NA NA NA NA +COLO-829-BL 2737 NA 35283 15358 0.44 0.00000 6809.59 6809.59 NA NA NA NA NA NA NA NA NA NA +COLO-829-BL 2739 NA 35283 21685 0.61 0.00000 9419.21 9419.21 NA NA NA NA NA NA NA NA NA NA 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NA NA NA NA NA +COLO-829-BL 2764 NA 35283 24581 0.70 0.00000 10365.04 10365.04 NA NA NA NA NA NA NA NA NA NA +COLO-829-BL 2767 NA 35283 24253 0.69 0.00000 10348.05 10348.05 NA NA NA NA NA NA NA NA NA NA +COLO-829-BL 2769 NA 35283 24453 0.69 0.00000 10321.45 10321.45 NA NA NA NA NA NA NA NA NA NA +COLO-829-BL 2772 NA 35283 23534 0.67 0.00000 10112.26 10112.26 NA NA NA NA NA NA NA NA NA NA +COLO-829-BL 2774 NA 35283 23461 0.66 0.00000 10083.68 10083.68 NA NA NA NA NA NA NA NA NA NA +COLO-829-BL 2776 NA 35283 24907 0.71 0.00000 10460.70 10460.70 NA NA NA NA NA NA NA NA NA NA +COLO-829-BL 2779 NA 35283 24558 0.70 0.00000 10413.08 10413.08 NA NA NA NA NA NA NA NA NA NA +COLO-829-BL 2781 NA 35283 21801 0.62 0.00000 9339.48 9339.48 NA NA NA NA NA NA NA NA NA NA +COLO-829-BL 2783 NA 35283 23326 0.66 0.00000 9874.64 9874.64 NA NA NA NA NA NA NA NA NA NA +COLO-829-BL 2785 NA 35283 22665 0.64 0.00000 9652.76 9652.76 NA NA NA NA NA NA NA NA NA NA +COLO-829-BL 2787 NA 35283 23602 0.67 0.00000 9966.43 9966.43 NA NA NA NA NA NA NA NA NA NA +COLO-829-BL 2790 NA 35283 23681 0.67 0.00000 10074.88 10074.88 NA NA NA NA NA NA NA NA NA NA +COLO-829-BL 2792 NA 35283 23065 0.65 0.00000 9725.33 9725.33 NA NA NA NA NA NA NA NA NA NA diff --git a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL.selfSM b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL.selfSM new file mode 100644 index 0000000..463644d --- /dev/null +++ b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL.selfSM @@ -0,0 +1,2 @@ +#SEQ_ID RG CHIP_ID #SNPS #READS AVG_DP FREEMIX FREELK1 FREELK0 FREE_RH FREE_RA CHIPMIX CHIPLK1 CHIPLK0 CHIP_RH CHIP_RA DPREF RDPHET RDPALT +COLO-829-BL ALL NA 35283 1489080 42.20 0.00075 336467.25 336532.29 NA NA NA NA NA NA NA NA NA NA diff --git a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL_snps.vcf b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL_snps.vcf new file mode 100644 index 0000000..cf49e8c --- /dev/null +++ b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL_snps.vcf @@ -0,0 +1,35408 @@ +#CHROM POS ID REF ALT QUAL FILTER INFO +1 1017216 rs6667248 A G . PASS AF=0.04188 +1 1311716 rs2649589 G A . PASS AF=0.77138 +1 1778090 rs12126768 T G . PASS AF=0.15348 +1 2069681 rs3753242 C T . PASS AF=0.19781 +1 2234251 rs1496555 A G . PASS AF=0.86161 +1 2328714 rs2645081 A G . PASS AF=0.84304 +1 2546229 rs6684865 G A . PASS AF=0.43266 +1 2831369 rs2606408 T C . PASS AF=0.05865 +1 2919942 rs12022429 G A . PASS AF=0.17887 +1 2974212 rs12057171 T C . PASS AF=0.02565 +1 3080855 rs1798246 A C . PASS AF=0.43709 +1 3138136 rs2817148 A G . PASS AF=0.13715 +1 3220160 rs6670518 A G . PASS AF=0.11327 +1 3256202 rs16824338 G A . PASS AF=0.04755 +1 3337552 rs2493283 T C . PASS AF=0.08286 +1 3494079 rs12080677 A G . PASS AF=0.22687 +1 3607520 rs3765730 G A . PASS AF=0.25503 +1 3696639 rs16824088 C T . PASS AF=0.02402 +1 3751929 rs12093010 G T . PASS AF=0.04990 +1 3999637 rs4654522 C G . PASS AF=0.33273 +1 4039440 rs12758410 C T . PASS AF=0.51987 +1 4111741 rs4553153 C T . PASS AF=0.25766 +1 4158734 rs12031803 T A . PASS AF=0.03986 +1 4220301 rs7549636 G A . PASS AF=0.24675 +1 4284659 rs903540 T C . PASS AF=0.68878 +1 4343581 rs16838549 G A . PASS AF=0.02398 +1 4370346 rs16838636 C A . PASS AF=0.01553 +1 4395020 rs12046130 G A . PASS AF=0.05761 +1 4426478 rs16838819 A C . PASS AF=0.18149 +1 4457112 rs16838929 C G . PASS AF=0.16709 +1 4482610 rs737324 G C . PASS AF=0.38945 +1 4523410 rs17453664 G A . PASS AF=0.12283 +1 4559484 rs616317 C A . PASS AF=0.07528 +1 4582089 rs518456 T G . PASS AF=0.35263 +1 4617856 rs10799244 T C . PASS AF=0.25289 +1 4657295 rs651695 A T . PASS AF=0.25761 +1 4693931 rs6687814 A G . PASS AF=0.40161 +1 4734514 rs12411035 A G . PASS AF=0.17888 +1 4774438 rs242058 T C . PASS AF=0.87270 +1 4812815 rs381240 A G . PASS AF=0.30488 +1 4853068 rs12408533 A G . PASS AF=0.03817 +1 4897644 rs11576972 G C . PASS AF=0.18922 +1 4958939 rs10915656 A G . PASS AF=0.22449 +1 4986435 rs4529681 G A . PASS AF=0.08583 +1 5011290 rs12403612 C G . PASS AF=0.07067 +1 5048624 rs7541612 T C . PASS AF=0.85640 +1 5088617 rs2169089 A G . PASS AF=0.76304 +1 5151476 rs2199215 T C . PASS AF=0.52212 +1 5198789 rs16840051 C T . PASS AF=0.34230 +1 5247355 rs10492931 G A . PASS AF=0.45884 +1 5303371 rs480807 G A . PASS AF=0.64359 +1 5330286 rs494138 C T . PASS AF=0.46626 +1 5362292 rs9439551 C G . PASS AF=0.38029 +1 5410542 rs4129058 C T . PASS AF=0.54484 +1 5532101 rs12566922 T G . PASS AF=0.14638 +1 5572076 rs10796398 G A . PASS AF=0.22742 +1 5625929 rs7523214 C G . PASS AF=0.05431 +1 5645712 rs4845805 G A . PASS AF=0.39912 +1 5684440 rs945321 C A . PASS AF=0.16142 +1 5718367 rs17028547 A C . PASS AF=0.02072 +1 5750582 rs770710 A T . PASS AF=0.16094 +1 5813724 rs12061902 T C . PASS AF=0.17633 +1 5902086 rs11579676 C T . PASS AF=0.21427 +1 5925955 rs12737596 T C . PASS AF=0.04300 +1 5985072 rs1011994 C T . PASS AF=0.14861 +1 6059322 rs7524865 G A . PASS AF=0.14348 +1 6141261 rs6701690 C G . PASS AF=0.10958 +1 6272813 rs2294711 C T . PASS AF=0.11485 +1 6416104 rs3789470 T C . PASS AF=0.10013 +1 6581283 rs12410405 C G . PASS AF=0.20773 +1 6713114 rs2235564 C T . PASS AF=0.31874 +1 6817343 rs17027008 T A . PASS AF=0.13993 +1 6927752 rs11120780 A G . PASS AF=0.63959 +1 7018719 rs11120797 A G . PASS AF=0.03063 +1 7089029 rs17030160 A C . PASS AF=0.01558 +1 7146809 rs9434834 T C . PASS AF=0.15858 +1 7214374 rs13376128 G T . PASS AF=0.22414 +1 7297400 rs7521056 A T . PASS AF=0.11816 +1 7336978 rs4908615 T A . PASS AF=0.43213 +1 7406419 rs4908456 T C . PASS AF=0.34129 +1 7449971 rs7524314 G A . PASS AF=0.29214 +1 7527996 rs1201529 T C . PASS AF=0.52139 +1 7554982 rs1725269 C T . PASS AF=0.70066 +1 7626995 rs17031145 C A . PASS AF=0.05192 +1 7697179 rs6661496 A T . PASS AF=0.09450 +1 7795554 rs4908688 C T . PASS AF=0.18933 +1 7850081 rs10864315 C T . PASS AF=0.26519 +1 7926542 rs504560 A G . PASS AF=0.64277 +1 8000562 rs9657956 C T . PASS AF=0.05221 +1 8161850 rs11487884 A G . PASS AF=0.08060 +1 8206326 rs6577473 C A . PASS AF=0.59929 +1 8273177 rs6683603 T G . PASS AF=0.32833 +1 8329710 rs2661869 C T . PASS AF=0.63665 +1 8457623 rs1443929 G T . PASS AF=0.15060 +1 8612104 rs6577499 G C . PASS AF=0.64178 +1 8767780 rs10864364 C A . PASS AF=0.85979 +1 8899305 rs6577532 C T . PASS AF=0.81602 +1 8993867 rs7417097 G A . PASS AF=0.30485 +1 9045124 rs7513804 C T . PASS AF=0.71809 +1 9116743 rs12080175 T C . PASS AF=0.30975 +1 9219383 rs6577556 T C . PASS AF=0.20481 +1 9292082 rs3753271 C T . PASS AF=0.30014 +1 9368209 rs1294040 G A . PASS AF=0.64682 +1 9417946 rs9435244 G A . PASS AF=0.10350 +1 9495587 rs9726405 T C . PASS AF=0.17077 +1 9650571 rs6669846 C T . PASS AF=0.02455 +1 9784423 rs11121484 C T . PASS AF=0.21830 +1 10013014 rs7516611 A C . PASS AF=0.15632 +1 10232200 rs17034569 C T . PASS AF=0.08874 +1 10353112 rs10492972 T C . PASS AF=0.32968 +1 10502710 rs2847337 A G . PASS AF=0.81899 +1 10591104 rs12090079 T C . PASS AF=0.07608 +1 10627542 rs12033275 G T . PASS AF=0.20859 +1 10726689 rs284321 G A . PASS AF=0.12132 +1 10832861 rs205474 C T . PASS AF=0.57717 +1 10929298 rs1281001 A G . PASS AF=0.59128 +1 11034817 rs9659356 A G . PASS AF=0.29462 +1 11191232 rs12732063 G A . PASS AF=0.01854 +1 11289161 rs2076655 G A . PASS AF=0.57495 +1 11401745 rs2982384 G A . PASS AF=0.37641 +1 11461601 rs6671983 G A . PASS AF=0.36952 +1 11507303 rs3010220 G A . PASS AF=0.02134 +1 11567190 rs2817637 C T . PASS AF=0.46179 +1 11647669 rs2817644 C T . PASS AF=0.31047 +1 11749908 rs6694489 C T . PASS AF=0.19377 +1 11828319 rs4845881 G A . PASS AF=0.65428 +1 11896654 rs198403 G A . PASS AF=0.12652 +1 11960394 rs17346334 T C . PASS AF=0.06100 +1 12117901 rs6665486 A G . PASS AF=0.46139 +1 12194612 rs1148471 G A . PASS AF=0.47178 +1 12249770 rs548580 G A . PASS AF=0.19639 +1 12418700 rs10864552 T C . PASS AF=0.94679 +1 12615069 rs6659122 G T . PASS AF=0.13976 +1 12672536 rs12046693 G C . PASS AF=0.21400 +1 12765166 rs4568844 C T . PASS AF=0.75593 +1 12813522 rs4548455 C T . PASS AF=0.05027 +1 13008676 rs3928862 G A . PASS AF=0.16698 +1 13841055 rs2990683 T C . PASS AF=0.65144 +1 13909142 rs355023 A C . PASS AF=0.63583 +1 13948047 rs12085273 G T . PASS AF=0.02610 +1 14043026 rs12049438 G A . PASS AF=0.17276 +1 14119085 rs1203642 T C . PASS AF=0.17732 +1 14150992 rs2697962 T C . PASS AF=0.82785 +1 14216150 rs17350970 C T . PASS AF=0.03696 +1 14252247 rs1534946 A G . PASS AF=0.12197 +1 14278991 rs7532844 T A . PASS AF=0.10033 +1 14313274 rs12728009 A G . PASS AF=0.18096 +1 14346018 rs7515012 C T . PASS AF=0.18178 +1 14380326 rs7519048 G C . PASS AF=0.14984 +1 14441384 rs7519796 A G . PASS AF=0.73920 +1 14474715 rs11586283 T C . PASS AF=0.33907 +1 14509276 rs577987 A G . PASS AF=0.15488 +1 14586318 rs10754915 T C . PASS AF=0.94610 +1 14655424 rs16853960 G A . PASS AF=0.04361 +1 14726096 rs820668 T C . PASS AF=0.96262 +1 14782380 rs6682103 T G . PASS AF=0.57814 +1 14811781 rs2480063 G A . PASS AF=0.45767 +1 14859579 rs7540545 T C . PASS AF=0.39903 +1 14919895 rs664747 G A . PASS AF=0.46653 +1 14981240 rs7530538 T C . PASS AF=0.59303 +1 15047171 rs10754867 G A . PASS AF=0.70954 +1 15089767 rs4543785 C T . PASS AF=0.38483 +1 15136508 rs3916544 C T . PASS AF=0.24768 +1 15192916 rs4661539 C A . PASS AF=0.44374 +1 15256903 rs12141312 T A . PASS AF=0.40972 +1 15295333 rs10927602 A G . PASS AF=0.51805 +1 15333053 rs4633284 C G . PASS AF=0.24429 +1 15360721 rs938255 C T . PASS AF=0.75659 +1 15431657 rs494983 G T . PASS AF=0.60133 +1 15500332 rs16851358 C T . PASS AF=0.16298 +1 15528667 rs17382617 G A . PASS AF=0.03972 +1 15566699 rs10927744 G A . PASS AF=0.87298 +1 15631649 rs17377023 G A . PASS AF=0.03249 +1 15701116 rs10927776 C T . PASS AF=0.02729 +1 15759547 rs6700655 T A . PASS AF=0.38681 +1 15843244 rs2042369 G A . PASS AF=0.21551 +1 15960608 rs11808281 G A . PASS AF=0.02708 +1 16087824 rs12091731 C T . PASS AF=0.25773 +1 16282776 rs4661346 G C . PASS AF=0.15663 +1 16379416 rs12015135 A C . PASS AF=0.18019 +1 16534646 rs221058 C G . PASS AF=0.18944 +1 16716374 rs612727 T C . PASS AF=0.73533 +1 16814994 rs156851 C T . PASS AF=0.93350 +1 17290058 rs6696928 A G . PASS AF=0.94176 +1 17386104 rs12404121 C T . PASS AF=0.55366 +1 17409241 rs2235925 G A . PASS AF=0.41606 +1 17465174 rs2746486 A G . PASS AF=0.53898 +1 17514734 rs3003464 C G . PASS AF=0.25143 +1 17557837 rs3003409 A C . PASS AF=0.39339 +1 17595925 rs17458091 T C . PASS AF=0.08699 +1 17655407 rs1748041 C T . PASS AF=0.63610 +1 17694701 rs1204890 T G . PASS AF=0.28934 +1 17785736 rs2428740 C T . PASS AF=0.34500 +1 17903298 rs6702679 G A . PASS AF=0.32566 +1 18003946 rs6665721 C T . PASS AF=0.02171 +1 18079812 rs12136469 C T . PASS AF=0.22986 +1 18143674 rs508571 C T . PASS AF=0.33696 +1 18201318 rs563835 T C . PASS AF=0.48431 +1 18246140 rs12073092 T A . PASS AF=0.31540 +1 18282095 rs1192583 G A . PASS AF=0.93892 +1 18317841 rs12726822 G A . PASS AF=0.20157 +1 18343106 rs12058837 G T . PASS AF=0.14041 +1 18374838 rs4920416 T C . PASS AF=0.99900 +1 18428766 rs1342572 G A . PASS AF=0.94886 +1 18468789 rs1455913 G A . PASS AF=0.29995 +1 18504792 rs4920448 T C . PASS AF=0.07059 +1 18553632 rs17489003 T C . PASS AF=0.21247 +1 18586815 rs402642 T C . PASS AF=0.37700 +1 18633357 rs12127986 G T . PASS AF=0.24380 +1 18678170 rs16861827 C T . PASS AF=0.11961 +1 18707373 rs10492995 A C . PASS AF=0.14486 +1 18761893 rs1850750 C T . PASS AF=0.12576 +1 18797703 rs2992736 A G . PASS AF=0.43313 +1 18839394 rs2294656 C T . PASS AF=0.27825 +1 18913558 rs4920334 T C . PASS AF=0.31500 +1 18995326 rs12141588 C T . PASS AF=0.02624 +1 19049615 rs2236817 C A . PASS AF=0.13805 +1 19094790 rs7545724 A G . PASS AF=0.05729 +1 19131080 rs7418296 G A . PASS AF=0.13208 +1 19203997 rs2230705 C G . PASS AF=0.64065 +1 19323050 rs6690097 G C . PASS AF=0.19334 +1 19400626 rs3748762 T C . PASS AF=0.04243 +1 19442508 rs4911974 C T . PASS AF=0.73542 +1 19564037 rs710879 C T . PASS AF=0.21175 +1 19645673 rs4912069 G A . PASS AF=0.24652 +1 19697915 rs10753558 G A . PASS AF=0.28179 +1 19734009 rs7512231 G A . PASS AF=0.05000 +1 19772847 rs10492998 C T . PASS AF=0.16305 +1 19809066 rs6703356 G A . PASS AF=0.36141 +1 19896220 rs3133373 C T . PASS AF=0.92459 +1 19988894 rs1977101 A G . PASS AF=0.15076 +1 20047186 rs3934190 T C . PASS AF=0.53637 +1 20092176 rs2281245 C T . PASS AF=0.17375 +1 20150984 rs4654865 C G . PASS AF=0.36410 +1 20187321 rs7531128 G A . PASS AF=0.05214 +1 20257351 rs12059920 T C . PASS AF=0.01680 +1 20306571 rs11573152 C T . PASS AF=0.01087 +1 20403098 rs11573222 G A . PASS AF=0.32173 +1 20452434 rs623784 A G . PASS AF=0.32409 +1 20544764 rs16823611 T C . PASS AF=0.05000 +1 20583060 rs11804672 C T . PASS AF=0.10455 +1 20631246 rs10753495 G A . PASS AF=0.77826 +1 20686162 rs230174 T C . PASS AF=0.70231 +1 20769184 rs750070 A C . PASS AF=0.45329 +1 20834738 rs610763 G C . PASS AF=0.17634 +1 20888348 rs657624 G A . PASS AF=0.22903 +1 20943195 rs12404655 A G . PASS AF=0.15579 +1 20985796 rs625149 G A . PASS AF=0.55371 +1 21047404 rs3738144 A G . PASS AF=0.15820 +1 21155195 rs2320590 C T . PASS AF=0.58429 +1 21281181 rs10916908 T A . PASS AF=0.05000 +1 21515906 rs6702110 A G . PASS AF=0.24856 +1 21621536 rs213051 T G . PASS AF=0.42972 +1 21669801 rs3026806 C T . PASS AF=0.05000 +1 21769997 rs10916992 G A . PASS AF=0.94033 +1 21863367 rs16825522 T A . PASS AF=0.01451 +1 21933193 rs829370 C T . PASS AF=0.92052 +1 21997282 rs3738091 G A . PASS AF=0.37796 +1 22067338 rs333174 G A . PASS AF=0.76417 +1 22198190 rs11586789 C T . PASS AF=0.08688 +1 22276058 rs12025633 C T . PASS AF=0.07590 +1 22358055 rs760922 T C . PASS AF=0.97601 +1 22443760 rs2235530 C T . PASS AF=0.07874 +1 22507423 rs2807346 C T . PASS AF=0.34045 +1 22547479 rs2505709 C T . PASS AF=0.46630 +1 22577056 rs16826845 A G . PASS AF=0.03396 +1 22636808 rs4589080 T C . PASS AF=0.52177 +1 22706579 rs1317353 G A . PASS AF=0.22591 +1 22814354 rs4655068 C T . PASS AF=0.36612 +1 22916671 rs10917263 G A . PASS AF=0.08912 +1 22986738 rs629409 C T . PASS AF=0.27781 +1 23060250 rs12566963 C T . PASS AF=0.01761 +1 23117460 rs10799764 C T . PASS AF=0.20689 +1 23164756 rs16827646 T G . PASS AF=0.30046 +1 23204183 rs12239985 G A . PASS AF=0.01306 +1 23271504 rs2903995 T A . PASS AF=0.36751 +1 23432346 rs1208930 C T . PASS AF=0.62800 +1 23540543 rs16828054 G A . PASS AF=0.14463 +1 23631714 rs2849021 T G . PASS AF=0.05086 +1 23712338 rs685895 G C . PASS AF=0.86794 +1 23765928 rs16828494 A G . PASS AF=0.04264 +1 23914063 rs2811948 T C . PASS AF=0.95779 +1 24004099 rs2864705 C T . PASS AF=0.72093 +1 24237631 rs3123558 A T . PASS AF=0.92270 +1 24404988 rs12719820 T C . PASS AF=0.43898 +1 24475252 rs4649190 G A . PASS AF=0.93798 +1 24541243 rs11249035 C T . PASS AF=0.09484 +1 24602626 rs12065087 C A . PASS AF=0.04940 +1 24641335 rs635539 A G . PASS AF=0.77805 +1 24713655 rs519782 C A . PASS AF=0.25138 +1 24785393 rs2272935 C T . PASS AF=0.31273 +1 24898453 rs6424106 C T . PASS AF=0.11986 +1 24967165 rs12072370 G A . PASS AF=0.01210 +1 25111040 rs3122056 T A . PASS AF=0.05097 +1 25207629 rs4649033 A G . PASS AF=0.37370 +1 25286151 rs4648888 C T . PASS AF=0.60942 +1 25329752 rs11249231 G A . PASS AF=0.16001 +1 25372257 rs311441 G C . PASS AF=0.88461 +1 25408685 rs12081177 G T . PASS AF=0.06313 +1 25459432 rs2753320 T C . PASS AF=0.32543 +1 25628088 rs1053360 G A . PASS AF=0.20051 +1 25940020 rs11247855 C G . PASS AF=0.12593 +1 26031014 rs1971442 G T . PASS AF=0.03898 +1 26118149 rs807250 G A . PASS AF=0.04826 +1 26224633 rs12037513 A G . PASS AF=0.37687 +1 26305225 rs4659400 T A . PASS AF=0.53198 +1 26391259 rs12089458 G A . PASS AF=0.02444 +1 26524706 rs9970046 A G . PASS AF=0.72390 +1 26622974 rs4659423 A C . PASS AF=0.18968 +1 26699103 rs11584185 T C . PASS AF=0.43476 +1 26804406 rs11576300 G C . PASS AF=0.15431 +1 26910754 rs4970510 T A . PASS AF=0.25670 +1 27272844 rs7866 C T . PASS AF=0.02852 +1 27488299 rs12402451 T C . PASS AF=0.23429 +1 27686063 rs7521552 G C . PASS AF=0.97833 +1 27934863 rs545502 C T . PASS AF=0.02937 +1 28015435 rs4609453 A G . PASS AF=0.14539 +1 28212806 rs11399 C T . PASS AF=0.09820 +1 28270737 rs11247730 T C . PASS AF=0.58822 +1 28424910 rs6678050 G C . PASS AF=0.63634 +1 28564279 rs9508 A G . PASS AF=0.33501 +1 28729142 rs5013826 T C . PASS AF=0.33449 +1 29154255 rs482692 T C . PASS AF=0.91562 +1 29242197 rs203278 T C . PASS AF=0.62424 +1 29472435 rs2452783 C T . PASS AF=0.26309 +1 29607279 rs4428852 A G . PASS AF=0.37073 +1 29681060 rs11585813 T A . PASS AF=0.68867 +1 29755757 rs9426351 A G . PASS AF=0.33119 +1 29795699 rs9426456 T C . PASS AF=0.07712 +1 29868794 rs6422671 A G . PASS AF=0.91130 +1 30115820 rs271328 G A . PASS AF=0.29650 +1 30149249 rs9661274 A G . PASS AF=0.24438 +1 30191185 rs7520938 G C . PASS AF=0.50072 +1 30221061 rs10914307 A G . PASS AF=0.35262 +1 30265652 rs6691847 T C . PASS AF=0.83797 +1 30341797 rs12032770 A C . PASS AF=0.90998 +1 30369659 rs10753261 C T . PASS AF=0.43055 +1 30410502 rs4949232 A C . PASS AF=0.10210 +1 30445631 rs267708 A G . PASS AF=0.68781 +1 30499408 rs267719 T C . PASS AF=0.67030 +1 30537232 rs867720 T C . PASS AF=0.25822 +1 30609989 rs11804863 C T . PASS AF=0.05989 +1 30660403 rs7523165 A G . PASS AF=0.37237 +1 30711065 rs12410878 C A . PASS AF=0.09866 +1 30750679 rs459233 A G . PASS AF=0.20536 +1 30803478 rs6425951 C T . PASS AF=0.42228 +1 30849034 rs1410870 G A . PASS AF=0.53004 +1 30893321 rs728340 C A . PASS AF=0.18267 +1 30930479 rs6670642 T C . PASS AF=0.04678 +1 30981125 rs454900 A G . PASS AF=0.95320 +1 31006103 rs4949613 A G . PASS AF=0.96596 +1 31045509 rs3104435 A G . PASS AF=0.81412 +1 31080261 rs1391379 T C . PASS AF=0.63212 +1 31118383 rs1924270 A G . PASS AF=0.23903 +1 31180714 rs912218 G A . PASS AF=0.15731 +1 31232076 rs1323444 G T . PASS AF=0.05000 +1 31308918 rs6693933 T C . 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a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/COLO-829.selfSM b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/COLO-829.selfSM new file mode 100644 index 0000000..d673731 --- /dev/null +++ b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/COLO-829.selfSM @@ -0,0 +1,2 @@ +#SEQ_ID RG CHIP_ID #SNPS #READS AVG_DP FREEMIX FREELK1 FREELK0 FREE_RH FREE_RA CHIPMIX CHIPLK1 CHIPLK0 CHIP_RH CHIP_RA DPREF RDPHET RDPALT +COLO-829 ALL NA 20887 1234173 59.09 0.00109 272092.96 272205.96 NA NA NA NA NA NA NA NA NA NA diff --git a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/COLO-829_snps.vcf b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/COLO-829_snps.vcf new file mode 100644 index 0000000..bbe9eb6 --- /dev/null +++ b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/COLO-829_snps.vcf @@ -0,0 +1,20961 @@ +#CHROM POS ID REF ALT QUAL FILTER INFO +1 144551842 rs1699721 C T . PASS AF=0.95000 +1 144945737 rs2996281 G T . PASS AF=0.68029 +1 145041016 rs2799329 G A . PASS AF=0.05000 +2 41404 rs10173732 G A . PASS AF=0.18223 +2 151464 rs381726 T C . PASS AF=0.51279 +2 210076 rs11692330 G C . PASS AF=0.34338 +2 282736 rs6708541 G T . PASS AF=0.67819 +2 355800 rs6740249 T C . PASS AF=0.68073 +2 454860 rs1511324 G A . PASS AF=0.25911 +2 548121 rs2685261 C T . PASS AF=0.73483 +2 589743 rs2683980 G A . PASS AF=0.11729 +2 614168 rs2947411 A G . PASS AF=0.84001 +2 637830 rs13393304 A G . PASS AF=0.87828 +2 669023 rs3187671 C T . PASS AF=0.11917 +2 759749 rs7596247 A G . PASS AF=0.27573 +2 825721 rs4358155 A C . PASS AF=0.85572 +2 877633 rs12466616 G A . PASS AF=0.52501 +2 943250 rs4971346 C T . PASS AF=0.26955 +2 1025239 rs10201045 A G . PASS AF=0.71192 +2 1077718 rs10470000 T C . PASS AF=0.99900 +2 1173710 rs4464311 T C . PASS AF=0.58960 +2 1311760 rs9678456 T C . PASS AF=0.07164 +2 1383237 rs4927650 T G . PASS AF=0.68697 +2 1432599 rs949834 G A . PASS AF=0.08284 +2 1473898 rs11897718 T A . PASS AF=0.22705 +2 1620721 rs12614899 C T . PASS AF=0.20106 +2 1696016 rs2288183 G A . PASS AF=0.62480 +2 1772199 rs13403413 G A . PASS AF=0.25325 +2 1838964 rs6747366 A G . PASS AF=0.50786 +2 1897522 rs12476079 G C . PASS AF=0.08354 +2 1974582 rs1864882 T G . PASS AF=0.51705 +2 2058426 rs6716149 C T . PASS AF=0.04490 +2 2204863 rs12471456 G A . PASS AF=0.28694 +2 2268764 rs6753548 C G . PASS AF=0.64530 +2 2355993 rs2551214 G T . PASS AF=0.57480 +2 2410541 rs2063549 C T . PASS AF=0.25024 +2 2474047 rs1706869 G T . PASS AF=0.08784 +2 2520736 rs1710503 C T . PASS AF=0.61866 +2 2538135 rs17039995 G A . PASS AF=0.02618 +2 2583815 rs10166891 T C . PASS AF=0.17379 +2 2608466 rs17496843 C T . PASS AF=0.17934 +2 2643137 rs7591104 C G . PASS AF=0.38903 +2 2687911 rs1470738 A G . PASS AF=0.61086 +2 2740902 rs9309697 A G . PASS AF=0.86916 +2 2787699 rs4146909 T C . PASS AF=0.67053 +2 2837549 rs11686670 C A . PASS AF=0.24521 +2 2872628 rs876299 A G . PASS AF=0.35077 +2 2898776 rs6724661 A G . PASS AF=0.03476 +2 2944589 rs935626 A G . PASS AF=0.05100 +2 3001674 rs1729936 C T . PASS AF=0.56846 +2 3084999 rs6754010 T C . PASS AF=0.05000 +2 3129553 rs17040861 G A . PASS AF=0.13359 +2 3193040 rs17682 G A . PASS AF=0.24028 +2 3242858 rs6548144 C T . PASS AF=0.91382 +2 3280842 rs2462956 C T . PASS AF=0.61137 +2 3371308 rs10189709 G C . PASS AF=0.68827 +2 3457097 rs10193454 G A . PASS AF=0.19286 +2 3511044 rs12052249 C A . PASS AF=0.30512 +2 3665146 rs11896016 T C . PASS AF=0.66625 +2 3702733 rs11902059 A G . PASS AF=0.09878 +2 3732546 rs6743352 T C . PASS AF=0.15799 +2 3780896 rs357953 C A . PASS AF=0.31458 +2 3815767 rs4849927 G A . PASS AF=0.68382 +2 3841699 rs12466058 T C . PASS AF=0.57616 +2 3896630 rs1902020 A C . PASS AF=0.25202 +2 3929278 rs7592385 A G . PASS AF=0.49176 +2 4030608 rs1494055 G T . PASS AF=0.33029 +2 4088831 rs12467317 G A . PASS AF=0.11411 +2 4137992 rs6759055 T G . PASS AF=0.10721 +2 4186566 rs4850056 T G . PASS AF=0.26714 +2 4270966 rs10204732 C T . PASS AF=0.21922 +2 4313449 rs7606619 T G . PASS AF=0.04784 +2 4352168 rs17552614 A C . PASS AF=0.08816 +2 4427111 rs11897967 A G . PASS AF=0.12831 +2 4502958 rs654853 T G . PASS AF=0.61706 +2 4583303 rs17562653 A G . PASS AF=0.03849 +2 4607508 rs6717561 C G . PASS AF=0.25649 +2 4659821 rs13006169 A G . PASS AF=0.16464 +2 4724464 rs10208517 C A . PASS AF=0.07951 +2 4773653 rs987689 C G . PASS AF=0.04467 +2 4822438 rs13395076 G A . PASS AF=0.12013 +2 4853967 rs1439818 A C . PASS AF=0.37512 +2 4894001 rs17020102 A G . PASS AF=0.07035 +2 4929220 rs1371485 G A . PASS AF=0.21523 +2 4973754 rs12711964 A G . PASS AF=0.46424 +2 5205484 rs12466042 C T . PASS AF=0.10444 +2 5253908 rs6720425 G A . PASS AF=0.05325 +2 5293171 rs17331611 C A . PASS AF=0.05168 +2 5377640 rs17333600 A G . PASS AF=0.42806 +2 5418064 rs1453783 C T . PASS AF=0.53083 +2 5456009 rs6752772 A T . PASS AF=0.69376 +2 5511339 rs16863669 C T . PASS AF=0.06308 +2 5547463 rs1659014 G C . PASS AF=0.94664 +2 5588619 rs16863779 T C . PASS AF=0.09016 +2 5645201 rs12614734 T C . PASS AF=0.53370 +2 5700470 rs13036193 A G . PASS AF=0.12032 +2 5773869 rs10195195 C A . PASS AF=0.04161 +2 5821748 rs4547512 G T . PASS AF=0.74475 +2 5870314 rs16864077 G A . PASS AF=0.04686 +2 5921095 rs16864193 C T . PASS AF=0.05000 +2 5968922 rs995949 A C . PASS AF=0.35181 +2 6002657 rs12471085 C T . PASS AF=0.15341 +2 6040560 rs4668813 A G . PASS AF=0.88704 +2 6072487 rs12470700 C T . PASS AF=0.13850 +2 6117771 rs12623457 T A . PASS AF=0.28371 +2 6165022 rs16864588 T C . PASS AF=0.01168 +2 6204312 rs12992673 G A . PASS AF=0.24981 +2 6247942 rs4233842 T A . PASS AF=0.91947 +2 6297054 rs6738047 G A . PASS AF=0.17991 +2 6343942 rs11897219 A G . PASS AF=0.02500 +2 6381852 rs10495535 G A . PASS AF=0.03955 +2 6398894 rs6734659 T A . PASS AF=0.82854 +2 6443516 rs921227 G A . PASS AF=0.61601 +2 6496322 rs16865067 G T . PASS AF=0.10499 +2 6550266 rs1462060 G A . PASS AF=0.13385 +2 6580645 rs3101047 C T . PASS AF=0.37607 +2 6622471 rs12475855 A T . PASS AF=0.29136 +2 6655781 rs16865292 T C . PASS AF=0.05327 +2 6701257 rs2061315 A G . PASS AF=0.47396 +2 6753497 rs11884506 G A . PASS AF=0.05000 +2 6794825 rs7564462 T G . PASS AF=0.43025 +2 6857712 rs6725067 T C . PASS AF=0.42506 +2 6891670 rs6721103 T C . PASS AF=0.14217 +2 6948715 rs771177 T C . PASS AF=0.95000 +2 7007620 rs2122103 C G . PASS AF=0.94399 +2 7069534 rs11674053 G A . PASS AF=0.01087 +2 7101720 rs771318 A G . PASS AF=0.02314 +2 7150330 rs447721 C T . PASS AF=0.41743 +2 7187738 rs7575969 A G . PASS AF=0.17086 +2 7229091 rs6431847 T C . PASS AF=0.66016 +2 7263133 rs10221865 G A . PASS AF=0.21458 +2 7322437 rs12151441 T C . PASS AF=0.18653 +2 7350916 rs4669156 C A . PASS AF=0.29344 +2 7407463 rs16866052 G T . PASS AF=0.27630 +2 7471732 rs6732990 G A . PASS AF=0.50513 +2 7533435 rs6756247 G A . PASS AF=0.50075 +2 7586436 rs11897766 T C . PASS AF=0.03812 +2 7647860 rs1907451 G T . PASS AF=0.21387 +2 7714913 rs6710248 T C . PASS AF=0.38644 +2 7812716 rs11682611 C T . PASS AF=0.15396 +2 7869555 rs772431 C T . PASS AF=0.95981 +2 7942491 rs7570944 T C . PASS AF=0.20037 +2 7997419 rs1861500 T C . PASS AF=0.68291 +2 8045363 rs16866529 T C . PASS AF=0.46225 +2 8094445 rs42208 C T . PASS AF=0.95000 +2 8115782 rs1974696 G A . PASS AF=0.53508 +2 8161538 rs16866571 A T . PASS AF=0.14358 +2 8227802 rs6759347 A C . PASS AF=0.46412 +2 8283740 rs6735900 G T . PASS AF=0.17881 +2 8373273 rs1034381 T C . PASS AF=0.60006 +2 8436099 rs346830 A G . PASS AF=0.32265 +2 8466486 rs10210006 C G . PASS AF=0.31655 +2 8501738 rs3097283 T C . PASS AF=0.82707 +2 8549910 rs6744484 A G . PASS AF=0.09584 +2 8579305 rs876861 G A . PASS AF=0.13562 +2 8613081 rs6739697 T C . PASS AF=0.10287 +2 8667160 rs6712259 A G . PASS AF=0.69392 +2 8707402 rs345898 C T . PASS AF=0.06196 +2 8753269 rs12993630 C T . PASS AF=0.41669 +2 8810012 rs3856461 C T . PASS AF=0.76617 +2 8927209 rs2033755 C T . PASS AF=0.95842 +2 9076600 rs7596539 C T . PASS AF=0.07031 +2 9226881 rs328628 C G . PASS AF=0.72862 +2 9274692 rs12622209 C A . PASS AF=0.57889 +2 9328737 rs7582342 G A . PASS AF=0.33991 +2 9398844 rs2709590 A G . PASS AF=0.48675 +2 9449556 rs6715973 G A . PASS AF=0.05734 +2 9503165 rs4669386 G A . PASS AF=0.22443 +2 9574542 rs12464664 T C . PASS AF=0.44989 +2 9662820 rs10929587 T A . PASS AF=0.51541 +2 9770050 rs13417081 A G . PASS AF=0.50379 +2 9807984 rs6713719 C T . PASS AF=0.12039 +2 9863969 rs12466375 G A . PASS AF=0.46238 +2 9908191 rs16867157 C T . PASS AF=0.04292 +2 9985606 rs287986 T C . PASS AF=0.17170 +2 10046283 rs4669490 A C . PASS AF=0.51479 +2 10098227 rs6708858 T C . PASS AF=0.45760 +2 10163328 rs10803714 T C . PASS AF=0.74683 +2 10275814 rs717886 T C . PASS AF=0.69530 +2 10364272 rs16855971 C T . PASS AF=0.21964 +2 10413431 rs17379538 T C . PASS AF=0.33045 +2 10468296 rs1003652 A C . PASS AF=0.20545 +2 10519847 rs12477018 G A . PASS AF=0.19458 +2 10566804 rs2270307 A G . PASS AF=0.21516 +2 10640667 rs11686084 G A . PASS AF=0.06033 +2 10688969 rs4668687 T G . PASS AF=0.54211 +2 10734703 rs11680669 T C . PASS AF=0.05014 +2 10845437 rs6738662 C G . PASS AF=0.02290 +2 10922516 rs1686482 A C . PASS AF=0.38370 +2 10943882 rs1734365 A G . PASS AF=0.44218 +2 10986305 rs1734394 C T . PASS AF=0.60591 +2 11018001 rs2884310 G A . PASS AF=0.75607 +2 11033567 rs4233891 T G . PASS AF=0.78153 +2 11085961 rs6735622 C G . PASS AF=0.35715 +2 11135121 rs10185930 G A . PASS AF=0.67054 +2 11162735 rs1349722 C T . PASS AF=0.33021 +2 11258601 rs11892684 G A . PASS AF=0.05675 +2 11366000 rs10167277 T A . PASS AF=0.38573 +2 11509480 rs4668722 G A . PASS AF=0.75652 +2 11562669 rs6708015 T C . PASS AF=0.37061 +2 11649547 rs2082830 G A . PASS AF=0.95818 +2 11706730 rs13383579 C T . PASS AF=0.05000 +2 11753955 rs10174816 T C . PASS AF=0.11310 +2 11815723 rs11676470 G T . PASS AF=0.13260 +2 11872755 rs6723479 T C . PASS AF=0.01577 +2 11938946 rs2577262 G A . PASS AF=0.35863 +2 12017371 rs893341 C T . PASS AF=0.81988 +2 12069382 rs2581084 T G . PASS AF=0.41315 +2 12110926 rs6712004 T C . PASS AF=0.42622 +2 12146345 rs7565113 T C . PASS AF=0.27709 +2 12201097 rs10929783 G C . PASS AF=0.24947 +2 12242056 rs6432262 T C . PASS AF=0.73327 +2 12272871 rs10201223 A C . PASS AF=0.33915 +2 12304843 rs3863945 G C . PASS AF=0.24243 +2 12326837 rs17618039 A T . PASS AF=0.37096 +2 12363549 rs10929794 G A . PASS AF=0.23819 +2 12418532 rs6432275 C T . PASS AF=0.88380 +2 12451586 rs1453490 C G . PASS AF=0.05000 +2 12495667 rs4417692 C T . PASS AF=0.05760 +2 12568996 rs10929808 G A . PASS AF=0.79180 +2 12624618 rs12614015 C T . PASS AF=0.49376 +2 12678708 rs16859063 A C . PASS AF=0.04673 +2 12745377 rs6729948 T C . PASS AF=0.02164 +2 12791196 rs6746789 A G . PASS AF=0.04840 +2 12855750 rs7593863 A G . PASS AF=0.06448 +2 12898480 rs13400230 T C . PASS AF=0.05000 +2 12969972 rs7558818 C T . PASS AF=0.61856 +2 13069079 rs7607052 C T . PASS AF=0.49573 +2 13126278 rs7590964 A G . PASS AF=0.31416 +2 13202306 rs11678695 G T . PASS AF=0.14675 +2 13310106 rs10182726 G A . PASS AF=0.34464 +2 13392540 rs388556 C G . PASS AF=0.28923 +2 13549728 rs10182743 A G . PASS AF=0.13245 +2 13617364 rs10495616 G A . PASS AF=0.27591 +2 13676862 rs2140525 A G . PASS AF=0.69861 +2 13698137 rs4669937 A G . PASS AF=0.69140 +2 13792992 rs1510832 G A . PASS AF=0.13877 +2 13863284 rs6432409 G A . PASS AF=0.74476 +2 13955627 rs7592520 C T . PASS AF=0.13996 +2 14056291 rs6742948 C T . PASS AF=0.35433 +2 14126218 rs12612782 C T . PASS AF=0.05988 +2 14204517 rs16861144 A T . PASS AF=0.02068 +2 14291599 rs7355592 G A . PASS AF=0.09892 +2 14332903 rs7600508 A G . PASS AF=0.46007 +2 14373024 rs16861506 C T . PASS AF=0.07195 +2 14433542 rs11684967 G A . PASS AF=0.23486 +2 14505949 rs10171046 T G . PASS AF=0.55445 +2 14571744 rs4145975 T C . PASS AF=0.44785 +2 14658959 rs6745064 G A . PASS AF=0.56939 +2 14696966 rs2675896 G C . PASS AF=0.67694 +2 14759577 rs16861922 G C . PASS AF=0.05257 +2 14851012 rs1434983 G C . PASS AF=0.08831 +2 14911179 rs10929918 G T . PASS AF=0.28127 +2 15000512 rs7570741 C T . PASS AF=0.06644 +2 15043800 rs4670039 G A . PASS AF=0.13109 +2 15091566 rs17368670 A C . PASS AF=0.16720 +2 15133451 rs10495643 C T . PASS AF=0.13434 +2 15181133 rs6720338 T C . PASS AF=0.34935 +2 15203160 rs1367489 C T . PASS AF=0.49166 +2 15270541 rs4668873 A G . PASS AF=0.88718 +2 15326834 rs2302940 T C . PASS AF=0.08263 +2 15409565 rs735447 A G . PASS AF=0.20144 +2 15497974 rs6727262 G A . PASS AF=0.70764 +2 15601441 rs7590340 C T . PASS AF=0.55127 +2 15722503 rs13391772 A G . PASS AF=0.53607 +2 15787986 rs807616 A G . PASS AF=0.99510 +2 15818041 rs7582338 A G . PASS AF=0.16074 +2 15867762 rs4668964 C T . PASS AF=0.20597 +2 15906778 rs2544535 T C . PASS AF=0.56530 +2 15944021 rs10185339 C T . PASS AF=0.09042 +2 15986586 rs11678237 C A . PASS AF=0.27315 +2 16040430 rs2081782 C T . PASS AF=0.00100 +2 16137237 rs3213827 G A . PASS AF=0.57285 +2 16199265 rs10173113 G A . PASS AF=0.27502 +2 16394600 rs13018508 C T . PASS AF=0.04299 +2 16435385 rs16982180 A G . PASS AF=0.14966 +2 16471243 rs340742 C A . PASS AF=0.69614 +2 16545353 rs6761668 A G . PASS AF=0.06015 +2 16606596 rs4832591 A G . PASS AF=0.80419 +2 16644988 rs11096574 T G . PASS AF=0.68678 +2 16680691 rs1430054 A G . PASS AF=0.80269 +2 16734284 rs12997119 T C . PASS AF=0.13911 +2 16803169 rs10188832 T C . PASS AF=0.36735 +2 16826386 rs6727449 T C . PASS AF=0.03403 +2 16896822 rs17645454 C T . PASS AF=0.16373 +2 16949316 rs11691792 A C . PASS AF=0.18156 +2 16983700 rs500053 T C . PASS AF=0.55870 +2 17037809 rs6727942 G T . PASS AF=0.41711 +2 17121628 rs2287517 A C . PASS AF=0.29777 +2 17222830 rs2880387 T A . PASS AF=0.35885 +2 17303956 rs10495659 A G . PASS AF=0.25631 +2 17369844 rs287295 C T . PASS AF=0.68407 +2 17444663 rs16983422 A G . 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HcmV?d00001 diff --git a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.annot.muts.vcf.gz.tbi b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.annot.muts.vcf.gz.tbi new file mode 100644 index 0000000..8b13789 --- /dev/null +++ b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.annot.muts.vcf.gz.tbi @@ -0,0 +1 @@ + diff --git a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.flagged.muts.vcf.gz b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.flagged.muts.vcf.gz new file mode 100644 index 0000000..8b13789 --- /dev/null +++ b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.flagged.muts.vcf.gz @@ -0,0 +1 @@ + diff --git a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.flagged.muts.vcf.gz.tbi 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b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/caveman/alg_bean @@ -0,0 +1,4 @@ +rd_pos 2.63;47.37;23.68;13.16;13.16 +base_qual 0-9;10-19;20-24;25-29;30-34;35-39;40-200 +map_qual 0-60;255-255;61-254 +lane 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diff --git a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/caveman/caveman.cfg.ini b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/caveman/caveman.cfg.ini new file mode 100644 index 0000000..7648e75 --- /dev/null +++ b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/caveman/caveman.cfg.ini @@ -0,0 +1,14 @@ +CWD=/var/spool/cwl +MUT_BAM=/var/spool/cwl/tmp/COLO-829.bam +NORM_BAM=/var/spool/cwl/tmp/COLO-829-BL.bam +REF_IDX=/var/spool/cwl/reference_files/genome.fa.fai +IGNORE=/var/spool/cwl/reference_files/caveman/HiDepth.tsv +ALG_FILE=/var/spool/cwl/WGS_COLO-829_vs_COLO-829-BL/caveman/tmpCaveman/alg_bean +RESULT_DIR=/var/spool/cwl/WGS_COLO-829_vs_COLO-829-BL/caveman/tmpCaveman/results +SPLIT_FILE=/var/spool/cwl/WGS_COLO-829_vs_COLO-829-BL/caveman/tmpCaveman/splitList +SW=0 +SE=0 +DUP=0 +NORMCN=/var/spool/cwl/tmp/norm.cn.bed +TUMCN=/var/spool/cwl/tmp/tum.cn.bed +VER=1.13.2 diff --git 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ziAT&XcuJHx+Y!@va?0FUQ{;*{uN~(={|%IMn>CLrogAN!rc}_nV*Wuz;fB zGDag~Oq$87W9ep@nGy?TOXP#&Lty^{pqD)8;S#y&nN#~03}^*b^@>i+US7dGnpX$N ztDPBoLv%X~WC+@HT1fxEDX$c()@-@gzq-foCj*tZ2Bo5c54rzi6o7fdW8%u;6p+dE zPu3QGk)*h_<@r(4{nD@BfzW*SBgYRzHasOwWi6P*Ub1xEvb0L>XJ>BTJLtI{G<1t? z(sk+3kzPd!dpU9ZgOQdn*N;CM&#p&$GyFy44%$1bvjfW&ouQFHEf zPwwV;UbW8Wsn+wphn7*#=X3lTzl_taX&Pv|y2fS!n;OUc}``Xtb{e0?? z<7Sm7r=OxOImjP~OIa0e@=+W-?e@Ce7}&2 cat << EOF @@ -57,7 +57,7 @@ $TO_MINIMISE To do this quickly run: - find expected -type f \( -iname \*.bai -o -iname \*.crai -o -iname \*.tbi -o -iname \*.md5 -o -iname prob_arr -o -iname \*.flagged.muts.vcf.gz -o -iname \*.muts.ids.vcf.gz -o -iname \*.flagged.vcf.gz \) -size +1c -exec bash -c 'echo > {}' \; + find expected -type f \( -iname \*.log -o -iname \*.brm.bam -o -iname \*.intermediates.tar.gz -o -iname \*.copynumber.txt.gz -o -iname \*.bw -o -iname \*.bai -o -iname \*.crai -o -iname \*.tbi -o -iname \*.md5 -o -iname prob_arr -o -iname \*.flagged.muts.vcf.gz -o -iname \*.muts.ids.vcf.gz -o -iname \*.flagged.vcf.gz \) -size +1c -exec bash -c 'echo > {}' \; EOF exit 1 From 5e3e1100e784165f6bc33cb6d1bb2ce517717ede Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 11 Apr 2018 14:11:46 +0100 Subject: [PATCH 49/57] Minor correction to syntax --- scripts/analysisWGS.sh | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/scripts/analysisWGS.sh b/scripts/analysisWGS.sh index 47bc4ff..b429da3 100755 --- a/scripts/analysisWGS.sh +++ b/scripts/analysisWGS.sh @@ -68,7 +68,7 @@ if [ -z ${CPU+x} ]; then fi PINDEL_CPU=$CPU -if [ $PINDEL_CPU -gt 8 ]; +if [ $PINDEL_CPU -gt 8 ]; then PINDEL_CPU=8 fi From 6c2ee2d10055c6cf1767651a5c8b68b24e9d91ba Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Sat, 14 Apr 2018 10:02:27 +0100 Subject: [PATCH 50/57] cert expired at ucsc, reported but waiting for fix isn't an option --- build/opt-build.sh | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/build/opt-build.sh b/build/opt-build.sh index 40f59c5..4c083d9 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -156,7 +156,7 @@ if [ ! -e $SETUP_DIR/BRASS.success ]; then fi if [ ! -e $SETUP_DIR/blat.success ]; then - curl -sSL --retry 10 $SOURCE_BLAT > distro.zip + curl -k -sSL --retry 10 $SOURCE_BLAT > distro.zip rm -rf distro/* unzip -d distro distro.zip cd distro/blatSrc From 31c16af87c1e96242050a2d595d7004b49e83b12 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Mon, 16 Apr 2018 13:08:18 +0100 Subject: [PATCH 51/57] create workspace is not found --- scripts/ds-cgpwgs.pl | 2 ++ 1 file changed, 2 insertions(+) diff --git a/scripts/ds-cgpwgs.pl b/scripts/ds-cgpwgs.pl index f8f9ea7..fe82580 100644 --- a/scripts/ds-cgpwgs.pl +++ b/scripts/ds-cgpwgs.pl @@ -62,6 +62,8 @@ printf "Options loaded: \n%s\n",Dumper(\%opts); +make_path($opts{'o'}) unless(-d $opts{'o'}); + # figure out if ref already unpacked: my $ref_area = $opts{'o'}.'/reference_files'; my $ref_unpack = 1; From fc0f05cc6d5823f5235506c5cad49263ebd22bd4 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Fri, 4 May 2018 15:43:41 +0100 Subject: [PATCH 52/57] Bump version of base image and update docs --- CHANGES.md | 4 ++-- Dockerfile | 2 +- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/CHANGES.md b/CHANGES.md index 2a88c64..41e0c2f 100644 --- a/CHANGES.md +++ b/CHANGES.md @@ -4,8 +4,8 @@ * Full support for BAM+CSI and CRAM+CRAI as inputs * Reorganisation to allow access of other tools or necessary bindings -* See dockstore-cgpmap 3.0.0 - 3.0.2 (primarily adds mismatchQc and threads to some tools) -* See dockstore-cgpwxs 3.0.0 - 3.0.1 (fragment based analysis) +* See dockstore-cgpmap 3.0.0 - 3.0.4 (primarily adds mismatchQc and threads to some tools) +* See dockstore-cgpwxs 3.0.0 - 3.0.3 (fragment based analysis) * You will need to update the reference pack to include the new flagging rules, see example `json` files * Using build stages to shrink images. diff --git a/Dockerfile b/Dockerfile index 6ffbb1d..ee3ee13 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM quay.io/wtsicgp/dockstore-cgpwxs:3.0.2 as builder +FROM quay.io/wtsicgp/dockstore-cgpwxs:3.0.3 as builder USER root From dd63e45eba3f7434fd1e671ec81f1b133aae3c78 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Fri, 4 May 2018 15:56:16 +0100 Subject: [PATCH 53/57] Resolves #19 --- scripts/ds-cgpwgs.pl | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/scripts/ds-cgpwgs.pl b/scripts/ds-cgpwgs.pl index fe82580..1f6b4c0 100644 --- a/scripts/ds-cgpwgs.pl +++ b/scripts/ds-cgpwgs.pl @@ -260,7 +260,7 @@ =head1 SYNOPSIS Optional parameters -species -sp Species name (may require quoting) - -assembly -a Reference assembly + -assembly -as Reference assembly -skipbb -sb Skip Battenberg allele counts -outdir -o Set the output folder [$HOME] -cores -c Set the number of cpu/cores available [default all]. From b37a82caa07ffc8ceccf921b461804021fa32d02 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Fri, 4 May 2018 16:07:30 +0100 Subject: [PATCH 54/57] Resolves #20 --- scripts/ds-cgpwgs.pl | 23 +++++++++++++++++++++++ 1 file changed, 23 insertions(+) diff --git a/scripts/ds-cgpwgs.pl b/scripts/ds-cgpwgs.pl index 1f6b4c0..12f073b 100644 --- a/scripts/ds-cgpwgs.pl +++ b/scripts/ds-cgpwgs.pl @@ -18,6 +18,7 @@ 'as' => undef, 'pu' => undef, 'pi' => undef, + ); GetOptions( 'h|help' => \$opts{'h'}, @@ -62,6 +63,26 @@ printf "Options loaded: \n%s\n",Dumper(\%opts); +if(! defined $opts{'tidx'} || $opts{'tidx'} eq q{}) { + die "-tidx not defined"; +} elsif(! -e $opts{'tidx'}) { + die "File not found for -tidx ($opts{tidx})"; +} + +if(! defined $opts{'nidx'} || $opts{'nidx'} eq q{}) { + die "-nidx not defined"; +} elsif(! -e $opts{'nidx'}) { + die "File not found for -nidx ($opts{nidx})"; +} + +if(! -e $opts{'t'}.'.bas') { + die "No co-located bas file found for tumour, expected at $opts{t}.bas"; +} + +if(! -e $opts{'n'}.'.bas') { + die "No co-located bas file found for normal, expected at $opts{n}.bas"; +} + make_path($opts{'o'}) unless(-d $opts{'o'}); # figure out if ref already unpacked: @@ -254,7 +275,9 @@ =head1 SYNOPSIS -cnv_sv -cs Path to CNV_SV*.tar.gz -subcl -sc Path to SUBCL*.tar.gz -tumour -t Tumour [CR|B]AM file + -tidx Tumour [CR|B]AM index (bai|csi|crai) -normal -n Normal [CR|B]AM file + -nidx Normal [CR|B]AM index (bai|csi|crai) -exclude -e Exclude these contigs from SNV/Indel analysis e.g. NC_007605,hs37d5,GL% From 89a3384981703599f388232f45edef901b906807 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 8 May 2018 09:00:54 +0100 Subject: [PATCH 55/57] Move to https for example datasets --- examples/cgpwgs/bam_bai.json | 12 ++++++------ examples/cgpwgs/bam_bai_pu_pl.json | 12 ++++++------ 2 files changed, 12 insertions(+), 12 deletions(-) diff --git a/examples/cgpwgs/bam_bai.json b/examples/cgpwgs/bam_bai.json index 6b9d5e2..fd60c16 100644 --- a/examples/cgpwgs/bam_bai.json +++ b/examples/cgpwgs/bam_bai.json @@ -1,26 +1,26 @@ { "reference": { - "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/core_ref_GRCh37d5.tar.gz", + "path": "http://ftp.sanger.ac.uk/pub/cancer/dockstore/human/core_ref_GRCh37d5.tar.gz", "class": "File" }, "annot": { - "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/VAGrENT_ref_GRCh37d5_ensembl_75.tar.gz", + "path": "http://ftp.sanger.ac.uk/pub/cancer/dockstore/human/VAGrENT_ref_GRCh37d5_ensembl_75.tar.gz", "class": "File" }, "snv_indel": { - "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/SNV_INDEL_ref_GRCh37d5-fragment.tar.gz", + "path": "http://ftp.sanger.ac.uk/pub/cancer/dockstore/human/SNV_INDEL_ref_GRCh37d5-fragment.tar.gz", "class": "File" }, "cnv_sv": { - "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/CNV_SV_ref_GRCh37d5_brass6+.tar.gz", + "path": "http://ftp.sanger.ac.uk/pub/cancer/dockstore/human/CNV_SV_ref_GRCh37d5_brass6+.tar.gz", "class": "File" }, "subcl": { - "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/SUBCL_ref_GRCh37d5.tar.gz", + "path": "http://ftp.sanger.ac.uk/pub/cancer/dockstore/human/SUBCL_ref_GRCh37d5.tar.gz", "class": "File" }, "qcset": { - "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/qcGenotype_GRCh37d5.tar.gz", + "path": "http://ftp.sanger.ac.uk/pub/cancer/dockstore/human/qcGenotype_GRCh37d5.tar.gz", "class": "File" }, "tumour": { diff --git a/examples/cgpwgs/bam_bai_pu_pl.json b/examples/cgpwgs/bam_bai_pu_pl.json index d31ad27..3cbe4ee 100644 --- a/examples/cgpwgs/bam_bai_pu_pl.json +++ b/examples/cgpwgs/bam_bai_pu_pl.json @@ -1,26 +1,26 @@ { "reference": { - "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/core_ref_GRCh37d5.tar.gz", + "path": "http://ftp.sanger.ac.uk/pub/cancer/dockstore/human/core_ref_GRCh37d5.tar.gz", "class": "File" }, "annot": { - "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/VAGrENT_ref_GRCh37d5_ensembl_75.tar.gz", + "path": "http://ftp.sanger.ac.uk/pub/cancer/dockstore/human/VAGrENT_ref_GRCh37d5_ensembl_75.tar.gz", "class": "File" }, "snv_indel": { - "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/SNV_INDEL_ref_GRCh37d5-fragment.tar.gz", + "path": "http://ftp.sanger.ac.uk/pub/cancer/dockstore/human/SNV_INDEL_ref_GRCh37d5-fragment.tar.gz", "class": "File" }, "cnv_sv": { - "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/CNV_SV_ref_GRCh37d5_brass6+.tar.gz", + "path": "http://ftp.sanger.ac.uk/pub/cancer/dockstore/human/CNV_SV_ref_GRCh37d5_brass6+.tar.gz", "class": "File" }, "subcl": { - "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/SUBCL_ref_GRCh37d5.tar.gz", + "path": "http://ftp.sanger.ac.uk/pub/cancer/dockstore/human/SUBCL_ref_GRCh37d5.tar.gz", "class": "File" }, "qcset": { - "path": "ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/human/qcGenotype_GRCh37d5.tar.gz", + "path": "http://ftp.sanger.ac.uk/pub/cancer/dockstore/human/qcGenotype_GRCh37d5.tar.gz", "class": "File" }, "tumour": { From a79d888493ea938a285a1b17e6aa8847848f928d Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 8 May 2018 15:08:07 +0100 Subject: [PATCH 56/57] Resolves #24 --- scripts/ds-cgpwgs.pl | 5 +++-- 1 file changed, 3 insertions(+), 2 deletions(-) diff --git a/scripts/ds-cgpwgs.pl b/scripts/ds-cgpwgs.pl index 12f073b..43d0c4c 100644 --- a/scripts/ds-cgpwgs.pl +++ b/scripts/ds-cgpwgs.pl @@ -18,7 +18,7 @@ 'as' => undef, 'pu' => undef, 'pi' => undef, - + 'cr' => 350000, ); GetOptions( 'h|help' => \$opts{'h'}, @@ -37,7 +37,7 @@ 'sp|species:s' => \$opts{'sp'}, 'as|assembly:s' => \$opts{'as'}, 'sb|skipbb' => \$opts{'sb'}, - 'cr|cavereads=i' => \$opts{'cr'}, + 'cr|cavereads:i' => \$opts{'cr'}, 'c|cores:i' => \$opts{'c'}, 'o|outdir:s' => \$opts{'o'}, 'pu|purity:f' => \$opts{'pu'}, @@ -289,6 +289,7 @@ =head1 SYNOPSIS -cores -c Set the number of cpu/cores available [default all]. -ploidy -pl Set the ploidy (rho) for ascat - requires purity -purity -pu Set the purity (psi) for ascat - requires ploidy + -cavereads -cr Reads target per caveman split section [350000] Other: -help -h Brief help message. From 141b6d3d3fe904808b64f523839f80e6536f9e61 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 8 May 2018 19:16:33 +0100 Subject: [PATCH 57/57] [ci skip] doc fixed issues --- CHANGES.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGES.md b/CHANGES.md index 41e0c2f..9409b98 100644 --- a/CHANGES.md +++ b/CHANGES.md @@ -10,6 +10,7 @@ files * Using build stages to shrink images. * remove legacy PRE/POST-EXEC from cgpbox days +* resolves #17, #18, #19, #20, #21, #24 ## 1.1.2

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