what does they mean in the params.txt

[huqingtao2018]

hi,
would you please tell me what does those parameters means in the params.txt?
crispra_downstream=50
crispra_upstream=400
crispri_downstream=50
crispri_upstream=300

Thank you~

[fheigwer]

hi there,
these are parameters that take action when purpose_exclusive=1 and purpose=CRISPRa or purpose=CRISPRi and govern in what range around the transcription start sites of the genes' transcripts sgRNA targets are searched.

Best,
Florian

[huqingtao2018]

Thank you very much for answering so quickly.
Best,
Qingtao Hu