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Seurat required for MUSIC::Input_preprocess_10X() #3

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gwaybio opened this issue Nov 3, 2020 · 0 comments
Open

Seurat required for MUSIC::Input_preprocess_10X() #3

gwaybio opened this issue Nov 3, 2020 · 0 comments

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@gwaybio
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gwaybio commented Nov 3, 2020

Hello, I am using this package, and I wanted to document a roadblock that I ran into in the event that others have this issue as well.

The following code throws the following error:

Error

Error in Read10X(directory): could not find function "Read10X"
Traceback:

1. Input_preprocess_10X(data_dir)

Code

library(Biostrings)
library(clusterProfiler)
library(devtools)
library(SAVER)
install_github("bm2-lab/MUSIC")
library(MUSIC)

# Provide directory of 10X data
data_dir <- "data/perturbseq/"
data <- Input_preprocess_10X(data_dir)

Solution

Luckily, the solution was simple: Install and load Seurat.

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