Skip to content

STAR 2.7.3a ______ 2019/10/08
Compare
Choose a tag to compare
@alexdobin alexdobin released this 08 Oct 19:14
· 366 commits to master since this release
2.7.3a
921a50b

Major new STARsolo features:

  • Output enhancements:
    • Summary.csv statistics output for raw and filtered cells useful for quick run quality assessment.
    • --soloCellFilter option for basic filtering of the cells, similar to the methods used by CellRanger 2.2.x.
  • Better compatibility with CellRanger 3.x.x:
    • --soloUMIfiltering MultiGeneUMI option introduced in CellRanger 3.x.x for filtering UMI collisions between different genes.
    • --soloCBmatchWLtype 1MM_multi_pseudocounts option, introduced in CellRanger 3.x.x, which slightly changes the posterior probability calculation for CB with 1 mismatch.
  • Velocyto spliced/unspliced/ambiguous quantification:
    • --soloFeatures Velocyto option to produce Spliced, Unspliced, and Ambiguous counts similar to the velocyto.py tool developed by LaManno et al. This option is under active development and the results may change in the future versions.
  • Support for complex barcodes, e.g. inDrop:
    • Complex barcodes in STARsolo with --soloType CB_UMI_Complex, --soloCBmatchWLtype --soloAdapterSequence, --soloAdapterMismatchesNmax, --soloCBposition,--soloUMIposition
  • BAM tags:
    • CB/UB for corrected CellBarcode/UMI
    • GX/GN for gene ID/name
  • STARsolo most up-to-date documentation.
Assets 2
Loading