From e7c2c61980c5735917b8f0f6babc83deb6442b89 Mon Sep 17 00:00:00 2001 From: nguyenhv Date: Tue, 7 Nov 2023 12:51:53 +0100 Subject: [PATCH 01/66] shorten the similar code parts in PRIME and MixMHCpred --- .../MHC_predictors/MixMHCpred/mixmhcpred.py | 73 ++--------------- neofox/MHC_predictors/prime.py | 78 +++---------------- neofox/__init__.py | 2 +- .../neoantigen_mhc_binding_annotator.py | 3 +- neofox/helpers/mhc_helper.py | 67 +++++++++++++++- 5 files changed, 87 insertions(+), 136 deletions(-) diff --git a/neofox/MHC_predictors/MixMHCpred/mixmhcpred.py b/neofox/MHC_predictors/MixMHCpred/mixmhcpred.py index e72aa830..6e3f5b5d 100755 --- a/neofox/MHC_predictors/MixMHCpred/mixmhcpred.py +++ b/neofox/MHC_predictors/MixMHCpred/mixmhcpred.py @@ -21,21 +21,16 @@ from pandas.errors import EmptyDataError from neofox.helpers.epitope_helper import EpitopeHelper from neofox.helpers.runner import Runner +from neofox.helpers.mhc_helper import ParsedMhcAlleles from neofox.model.mhc_parser import MhcParser from neofox.model.neoantigen import Annotation, Mhc1, MhcAllele, PredictedEpitope, Neoantigen from neofox.model.factories import AnnotationFactory from neofox.helpers import intermediate_files -import pandas as pd import os from logzero import logger from neofox.references.references import DependenciesConfiguration -ALLELE = "BestAllele" -RANK = "%Rank_" -PEPTIDE = "Peptide" -SCORE = "Score_" - class MixMHCpred: @@ -45,65 +40,11 @@ class MixMHCpred: def __init__(self, runner: Runner, configuration: DependenciesConfiguration, mhc_parser: MhcParser): self.runner = runner self.configuration = configuration - self.available_alleles = self._load_available_alleles() self.mhc_parser = mhc_parser - self.results = None - - def _load_available_alleles(self): - """ - loads file with available HLA II alllels for MixMHC2pred prediction, returns set - :return: - """ - alleles = pd.read_csv( - self.configuration.mix_mhc_pred_alleles_list, sep="\t" - ) - return set(alleles["Allele"]) - - def _get_mixmhc_allele_representation(self, mhc_alleles: List[MhcAllele]): - return list( - filter( - lambda y: y in self.available_alleles, - map( - lambda x: "{gene}{group}{protein}".format(gene=x.gene, group=x.group, protein=x.protein), - mhc_alleles) - ) - ) - - def _get_mhc_alleles(self, mixmhc_result): - mhc_alleles = set() - for col in mixmhc_result.columns: - # take out alleles and eliminate the column Score_bestAllele out of the set - if col.startswith(SCORE) and not col.endswith('e'): - allele = col.split('_')[-1] - mhc_alleles.add(allele) - return mhc_alleles + self.parsed_mhc_alleles = ParsedMhcAlleles(mhc_parser) - def _parse_mixmhcpred_output(self, filename: str) -> List[PredictedEpitope]: - parsed_results = [] - try: - results = pd.read_csv(filename, sep="\t", comment="#") - except EmptyDataError: - logger.error("Results from MixMHCpred are empty, something went wrong") - results = pd.DataFrame() - - mhc_alleles = self._get_mhc_alleles(results) - for _, row in results.iterrows(): - # when MixMHCpred returns no results it provides a row with the peptide and NAs for other fields - # pandas reads NAs as float nan. Skip these - for allele in mhc_alleles: - if isinstance(row[PEPTIDE], str): - score = str(SCORE + allele) - rank = str(RANK + allele) - - parsed_results.append( - PredictedEpitope( - allele_mhc_i=self.mhc_parser.parse_mhc_allele(allele), - mutated_peptide=row[PEPTIDE], - affinity_mutated=float(row[score]), - rank_mutated=float(row[rank]), - )) - return parsed_results + self.results = None def _mixmhcprediction(self, mhc_alleles: List[str], potential_ligand_sequences) -> List[PredictedEpitope]: """ @@ -125,7 +66,7 @@ def _mixmhcprediction(self, mhc_alleles: List[str], potential_ligand_sequences) self.runner.run_command( cmd=command ) - results = self._parse_mixmhcpred_output(filename=outtmp) + results = self.parsed_mhc_alleles.parse_mixmhcpred_prime_output(mixmhc_prime_result=outtmp) os.remove(outtmp) os.remove(tmpfasta) return results @@ -140,7 +81,8 @@ def run(self, neoantigen: Neoantigen, mhc: List[Mhc1], uniprot): neoantigen=neoantigen, lengths=[8, 9, 10, 11, 12, 13, 14], uniprot=uniprot ) if len(potential_ligand_sequences) > 0: - mhc1_alleles = self._get_mixmhc_allele_representation([a for m in mhc for a in m.alleles]) + mhc1_alleles = self.parsed_mhc_alleles.get_mixmhc_allele_representation(self.configuration.mix_mhc_pred_alleles_list, + [a for m in mhc for a in m.alleles]) if len(mhc1_alleles) > 0: self.results = self._mixmhcprediction(mhc1_alleles, potential_ligand_sequences) else: @@ -149,7 +91,8 @@ def run(self, neoantigen: Neoantigen, mhc: List[Mhc1], uniprot): def run_peptide(self, peptide: str, allele: MhcAllele) -> PredictedEpitope: """Runs MixMHCpred on a single peptide""" result = None - mhc1_alleles = self._get_mixmhc_allele_representation([allele]) + mhc1_alleles = self.parsed_mhc_alleles.get_mixmhc_allele_representation(self.configuration.mix_mhc_pred_alleles_list, + [allele]) if len(mhc1_alleles) > 0 and 8 <= len(peptide) <= 14: results = self._mixmhcprediction(mhc1_alleles, [peptide]) if results: diff --git a/neofox/MHC_predictors/prime.py b/neofox/MHC_predictors/prime.py index ae97a1d3..c529abcb 100755 --- a/neofox/MHC_predictors/prime.py +++ b/neofox/MHC_predictors/prime.py @@ -19,98 +19,40 @@ # along with this program. If not, see .# from typing import List -from pandas.errors import EmptyDataError +#from pandas.errors import EmptyDataError from neofox.helpers.epitope_helper import EpitopeHelper from neofox.helpers.runner import Runner from neofox.model.mhc_parser import MhcParser +from neofox.helpers.mhc_helper import ParsedMhcAlleles from neofox.model.neoantigen import Annotation, Mhc1, MhcAllele, PredictedEpitope, Neoantigen from neofox.model.factories import AnnotationFactory from neofox.helpers import intermediate_files -import pandas as pd import os from logzero import logger from neofox.references.references import DependenciesConfiguration -ALLELE = "BestAllele" -RANK = "%Rank_" -PEPTIDE = "Peptide" -SCORE = "Score_" - - class Prime: ANNOTATION_PREFIX = 'PRIME' ANNOTATION_PREFIX_WT = 'PRIME_WT' - def __init__(self, runner: Runner, configuration: DependenciesConfiguration, mhc_parser: MhcParser): + def __init__(self, runner: Runner, configuration: DependenciesConfiguration, mhc_parser: MhcParser, + ): self.runner = runner self.configuration = configuration - self.available_alleles = self._load_available_alleles() self.mhc_parser = mhc_parser + self.parsed_mhc_alleles = ParsedMhcAlleles(mhc_parser) + self.best_peptide = None self.best_rank = None self.best_allele = None self.best_score = None self.results = None - def _load_available_alleles(self): - """ - loads file with available HLA II alleles for Prime prediction, returns set - :return: - """ - alleles = pd.read_csv( - self.configuration.prime_alleles_list, sep="\t", header=None, names=['Allele'] - ) - return list(alleles["Allele"]) - - def _get_mixmhc_allele_representation(self, mhc_alleles: List[MhcAllele]): - return list( - filter( - lambda y: y in self.available_alleles, - map( - lambda x: "{gene}{group}{protein}".format(gene=x.gene, group=x.group, protein=x.protein), - mhc_alleles) - ) - ) - def _get_mhc_alleles(self, prime_result): - mhc_alleles = set() - for col in prime_result.columns: - # take out alleles and eliminate the column Score_bestAllele out of the set - if col.startswith(SCORE) and not col.endswith('e'): - allele = col.split('_')[-1] - mhc_alleles.add(allele) - return mhc_alleles - - def _parse_prime_output(self, filename: str) -> List[PredictedEpitope]: - parsed_results = [] - try: - results = pd.read_csv(filename, sep="\t", comment="#") - except EmptyDataError: - logger.error("Results from MixMHCpred are empty, something went wrong") - results = pd.DataFrame() - - mhc_alleles = self._get_mhc_alleles(results) - for _, row in results.iterrows(): - # when MixMHCpred returns no results it provides a row with the peptide and NAs for other fields - # pandas reads NAs as float nan. Skip these - for allele in mhc_alleles: - if isinstance(row[PEPTIDE], str): - score = str(SCORE + allele) - rank = str(RANK + allele) - - parsed_results.append( - PredictedEpitope( - allele_mhc_i=self.mhc_parser.parse_mhc_allele(allele), - mutated_peptide=row[PEPTIDE], - affinity_mutated=float(row[score]), - rank_mutated=float(row[rank]), - )) - return parsed_results - def _prime(self, mhc_alleles: List[str], potential_ligand_sequences) -> List[PredictedEpitope]: """ Runs PRIME for desired hla allele and writes result to temporary file. @@ -133,7 +75,7 @@ def _prime(self, mhc_alleles: List[str], potential_ligand_sequences) -> List[Pre self.runner.run_command( cmd=command ) - results = self._parse_prime_output(filename=outtmp) + results = self.parsed_mhc_alleles.parse_mixmhcpred_prime_output(mixmhc_prime_result=outtmp) os.remove(outtmp) os.remove(tmpfasta) return results @@ -148,7 +90,8 @@ def run(self, neoantigen: Neoantigen, mhc: List[Mhc1], uniprot): neoantigen=neoantigen, lengths=[8, 9, 10, 11, 12, 13, 14], uniprot=uniprot ) if len(potential_ligand_sequences) > 0: - mhc1_alleles = self._get_mixmhc_allele_representation([a for m in mhc for a in m.alleles]) + mhc1_alleles = self.parsed_mhc_alleles.get_mixmhc_allele_representation(self.configuration.prime_alleles_list, + [a for m in mhc for a in m.alleles]) if len(mhc1_alleles) > 0: self.results = self._prime(mhc1_alleles, potential_ligand_sequences) else: @@ -157,7 +100,8 @@ def run(self, neoantigen: Neoantigen, mhc: List[Mhc1], uniprot): def run_peptide(self, peptide: str, allele: MhcAllele) -> PredictedEpitope: result = None if not EpitopeHelper.contains_rare_amino_acid(peptide=peptide): - mhc1_alleles = self._get_mixmhc_allele_representation([allele]) + mhc1_alleles = self.parsed_mhc_alleles.get_mixmhc_allele_representation(self.configuration.prime_alleles_list, + [allele]) if len(mhc1_alleles) > 0: results = self._prime(mhc1_alleles, [peptide]) if results: diff --git a/neofox/__init__.py b/neofox/__init__.py index 41b52e37..5939297b 100755 --- a/neofox/__init__.py +++ b/neofox/__init__.py @@ -18,7 +18,7 @@ # along with this program. If not, see .# -VERSION = "1.1.1" +VERSION = "1.1.1dev1" REFERENCE_FOLDER_ENV = "NEOFOX_REFERENCE_FOLDER" NEOFOX_BLASTP_ENV = "NEOFOX_BLASTP" diff --git a/neofox/annotator/neoantigen_mhc_binding_annotator.py b/neofox/annotator/neoantigen_mhc_binding_annotator.py index 9394a2aa..64d5fbf3 100644 --- a/neofox/annotator/neoantigen_mhc_binding_annotator.py +++ b/neofox/annotator/neoantigen_mhc_binding_annotator.py @@ -65,7 +65,6 @@ def get_mhc_binding_annotations(self, neoantigen: Neoantigen, patient: Patient): self.mhc_parser, neoantigen, patient, - self.mhc_database, self.references ) @@ -85,6 +84,7 @@ def get_mhc_binding_annotations(self, neoantigen: Neoantigen, patient: Patient): self.runner, self.configuration, self.mhc_parser, + neoantigen, patient, ) @@ -161,7 +161,6 @@ def _run_mixmhc2pred( mhc_parser: MhcParser, neoantigen: Neoantigen, patient: Patient, - mhc_database: MhcDatabase, references: ReferenceFolder ): mixmhc2 = MixMHC2pred(runner, configuration, mhc_parser, references) diff --git a/neofox/helpers/mhc_helper.py b/neofox/helpers/mhc_helper.py index edd1c090..be2f2cfb 100644 --- a/neofox/helpers/mhc_helper.py +++ b/neofox/helpers/mhc_helper.py @@ -1,6 +1,10 @@ from typing import List -from neofox.model.neoantigen import Mhc1, Zygosity +from neofox.model.neoantigen import Mhc1, Zygosity, MhcAllele, PredictedEpitope, Neoantigen +from neofox.model.mhc_parser import MhcParser +import pandas as pd +from pandas.errors import EmptyDataError +from logzero import logger class MhcHelper: @@ -29,3 +33,64 @@ def get_heterozygous_or_hemizygous_mhc1_alleles(mhc_isoforms: List[Mhc1]) -> Lis for a in m.alleles if m.zygosity in [Zygosity.HETEROZYGOUS, Zygosity.HEMIZYGOUS] ] + +class ParsedMhcAlleles: + + def __init__(self, mhc_parser: MhcParser): + self.mhc_parser = mhc_parser + + @staticmethod + def get_mixmhc_allele_representation(file_alleles, mhc_alleles: List[MhcAllele]): + """ + loads file with available HLA II alllels for MixMHC2pred prediction, returns set + :return: + """ + alleles = pd.read_csv( + file_alleles, sep="\t" + ) + available_alleles = set(alleles["Allele"]) + return list( + filter( + lambda y: y in available_alleles, + map( + lambda x: "{gene}{group}{protein}".format(gene=x.gene, group=x.group, protein=x.protein), + mhc_alleles) + ) + ) + + def parse_mixmhcpred_prime_output(self, mixmhc_prime_result) -> List[PredictedEpitope]: + RANK = "%Rank_" + PEPTIDE = "Peptide" + SCORE = "Score_" + + parsed_results = [] + + try: + results = pd.read_csv(mixmhc_prime_result, sep="\t", comment="#") + except EmptyDataError: + logger.error("Results from MixMHCpred are empty, something went wrong") + results = pd.DataFrame() + + mhc_alleles = set() + for col in results.columns: + # take out alleles and eliminate the column Score_bestAllele out of the set + if col.startswith(SCORE) and not col.endswith('e'): + allele = col.split('_')[-1] + mhc_alleles.add(allele) + + for _, row in results.iterrows(): + # when MixMHCpred returns no results it provides a row with the peptide and NAs for other fields + # pandas reads NAs as float nan. Skip these + for allele in mhc_alleles: + if isinstance(row[PEPTIDE], str): + score = str(SCORE + allele) + rank = str(RANK + allele) + + parsed_results.append( + PredictedEpitope( + allele_mhc_i=self.mhc_parser.parse_mhc_allele(allele), + mutated_peptide=row[PEPTIDE], + affinity_mutated=float(row[score]), + rank_mutated=float(row[rank]), + )) + return parsed_results \ No newline at end of file From 34c1351fafd8edaa1f641de2ec15430efcab56a2 Mon Sep 17 00:00:00 2001 From: nguyenhv Date: Tue, 7 Nov 2023 13:04:58 +0100 Subject: [PATCH 02/66] version of init --- neofox/__init__.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/neofox/__init__.py b/neofox/__init__.py index 5939297b..a53f0d0b 100755 --- a/neofox/__init__.py +++ b/neofox/__init__.py @@ -18,7 +18,7 @@ # along with this program. If not, see .# -VERSION = "1.1.1dev1" +VERSION = "1.1.1.dev1" REFERENCE_FOLDER_ENV = "NEOFOX_REFERENCE_FOLDER" NEOFOX_BLASTP_ENV = "NEOFOX_BLASTP" From c0d94dae8f9df134c6ed071737af6b7724720f4a Mon Sep 17 00:00:00 2001 From: nguyenhv Date: Tue, 7 Nov 2023 13:25:58 +0100 Subject: [PATCH 03/66] update mini mistakes --- neofox/MHC_predictors/MixMHCpred/mixmhcpred.py | 2 -- neofox/MHC_predictors/prime.py | 2 -- neofox/annotator/neoantigen_mhc_binding_annotator.py | 3 +-- 3 files changed, 1 insertion(+), 6 deletions(-) diff --git a/neofox/MHC_predictors/MixMHCpred/mixmhcpred.py b/neofox/MHC_predictors/MixMHCpred/mixmhcpred.py index 6e3f5b5d..c19935d5 100755 --- a/neofox/MHC_predictors/MixMHCpred/mixmhcpred.py +++ b/neofox/MHC_predictors/MixMHCpred/mixmhcpred.py @@ -18,7 +18,6 @@ # You should have received a copy of the GNU General Public License # along with this program. If not, see .# from typing import List -from pandas.errors import EmptyDataError from neofox.helpers.epitope_helper import EpitopeHelper from neofox.helpers.runner import Runner from neofox.helpers.mhc_helper import ParsedMhcAlleles @@ -41,7 +40,6 @@ def __init__(self, runner: Runner, configuration: DependenciesConfiguration, mhc self.runner = runner self.configuration = configuration self.mhc_parser = mhc_parser - self.parsed_mhc_alleles = ParsedMhcAlleles(mhc_parser) self.results = None diff --git a/neofox/MHC_predictors/prime.py b/neofox/MHC_predictors/prime.py index c529abcb..142db099 100755 --- a/neofox/MHC_predictors/prime.py +++ b/neofox/MHC_predictors/prime.py @@ -19,8 +19,6 @@ # along with this program. If not, see .# from typing import List -#from pandas.errors import EmptyDataError - from neofox.helpers.epitope_helper import EpitopeHelper from neofox.helpers.runner import Runner from neofox.model.mhc_parser import MhcParser diff --git a/neofox/annotator/neoantigen_mhc_binding_annotator.py b/neofox/annotator/neoantigen_mhc_binding_annotator.py index 64d5fbf3..f7cf2aeb 100644 --- a/neofox/annotator/neoantigen_mhc_binding_annotator.py +++ b/neofox/annotator/neoantigen_mhc_binding_annotator.py @@ -8,7 +8,7 @@ from neofox.helpers.runner import Runner from neofox.model.mhc_parser import MhcParser from neofox.model.neoantigen import Neoantigen, Patient -from neofox.references.references import DependenciesConfiguration, AvailableAlleles, ReferenceFolder, MhcDatabase, \ +from neofox.references.references import DependenciesConfiguration, AvailableAlleles, ReferenceFolder, \ ORGANISM_HOMO_SAPIENS @@ -84,7 +84,6 @@ def get_mhc_binding_annotations(self, neoantigen: Neoantigen, patient: Patient): self.runner, self.configuration, self.mhc_parser, - neoantigen, patient, ) From 2138b7c89ebabb25abc1f6eaabd2a8990942a6f0 Mon Sep 17 00:00:00 2001 From: nguyenhv Date: Tue, 7 Nov 2023 15:56:12 +0100 Subject: [PATCH 04/66] update selection based on RANK --- neofox/MHC_predictors/prime.py | 2 +- neofox/__init__.py | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/neofox/MHC_predictors/prime.py b/neofox/MHC_predictors/prime.py index 142db099..b1af6c6e 100755 --- a/neofox/MHC_predictors/prime.py +++ b/neofox/MHC_predictors/prime.py @@ -110,7 +110,7 @@ def run_peptide(self, peptide: str, allele: MhcAllele) -> PredictedEpitope: def get_annotations(self) -> List[Annotation]: - best_result = EpitopeHelper.select_best_by_affinity(predictions=self.results, maximum=True) + best_result = EpitopeHelper.select_best_by_rank(predictions=self.results) return [ AnnotationFactory.build_annotation( value=best_result.mutated_peptide, name="PRIME_best_peptide" diff --git a/neofox/__init__.py b/neofox/__init__.py index a53f0d0b..b86ecb14 100755 --- a/neofox/__init__.py +++ b/neofox/__init__.py @@ -18,7 +18,7 @@ # along with this program. If not, see .# -VERSION = "1.1.1.dev1" +VERSION = "1.1.1.dev2" REFERENCE_FOLDER_ENV = "NEOFOX_REFERENCE_FOLDER" NEOFOX_BLASTP_ENV = "NEOFOX_BLASTP" From 31e0123d3cd537222d9e923b5154ab505cb34867 Mon Sep 17 00:00:00 2001 From: nguyenhv Date: Wed, 8 Nov 2023 10:48:08 +0100 Subject: [PATCH 05/66] change the name of class --- neofox/MHC_predictors/MixMHCpred/mixmhcpred.py | 4 ++-- neofox/MHC_predictors/prime.py | 6 +++--- neofox/helpers/mhc_helper.py | 2 +- 3 files changed, 6 insertions(+), 6 deletions(-) diff --git a/neofox/MHC_predictors/MixMHCpred/mixmhcpred.py b/neofox/MHC_predictors/MixMHCpred/mixmhcpred.py index c19935d5..a4c85fcd 100755 --- a/neofox/MHC_predictors/MixMHCpred/mixmhcpred.py +++ b/neofox/MHC_predictors/MixMHCpred/mixmhcpred.py @@ -20,7 +20,7 @@ from typing import List from neofox.helpers.epitope_helper import EpitopeHelper from neofox.helpers.runner import Runner -from neofox.helpers.mhc_helper import ParsedMhcAlleles +from neofox.helpers.mhc_helper import MixMhcHelper from neofox.model.mhc_parser import MhcParser from neofox.model.neoantigen import Annotation, Mhc1, MhcAllele, PredictedEpitope, Neoantigen from neofox.model.factories import AnnotationFactory @@ -40,7 +40,7 @@ def __init__(self, runner: Runner, configuration: DependenciesConfiguration, mhc self.runner = runner self.configuration = configuration self.mhc_parser = mhc_parser - self.parsed_mhc_alleles = ParsedMhcAlleles(mhc_parser) + self.parsed_mhc_alleles = MixMhcHelper(mhc_parser) self.results = None diff --git a/neofox/MHC_predictors/prime.py b/neofox/MHC_predictors/prime.py index 142db099..f4f829c9 100755 --- a/neofox/MHC_predictors/prime.py +++ b/neofox/MHC_predictors/prime.py @@ -21,9 +21,9 @@ from neofox.helpers.epitope_helper import EpitopeHelper from neofox.helpers.runner import Runner -from neofox.model.mhc_parser import MhcParser -from neofox.helpers.mhc_helper import ParsedMhcAlleles +from neofox.helpers.mhc_helper import MixMhcHelper +from neofox.model.mhc_parser import MhcParser from neofox.model.neoantigen import Annotation, Mhc1, MhcAllele, PredictedEpitope, Neoantigen from neofox.model.factories import AnnotationFactory from neofox.helpers import intermediate_files @@ -43,7 +43,7 @@ def __init__(self, runner: Runner, configuration: DependenciesConfiguration, mhc self.configuration = configuration self.mhc_parser = mhc_parser - self.parsed_mhc_alleles = ParsedMhcAlleles(mhc_parser) + self.parsed_mhc_alleles = MixMhcHelper(mhc_parser) self.best_peptide = None self.best_rank = None diff --git a/neofox/helpers/mhc_helper.py b/neofox/helpers/mhc_helper.py index be2f2cfb..b4a9e13e 100644 --- a/neofox/helpers/mhc_helper.py +++ b/neofox/helpers/mhc_helper.py @@ -34,7 +34,7 @@ def get_heterozygous_or_hemizygous_mhc1_alleles(mhc_isoforms: List[Mhc1]) -> Lis if m.zygosity in [Zygosity.HETEROZYGOUS, Zygosity.HEMIZYGOUS] ] -class ParsedMhcAlleles: +class MixMhcHelper: def __init__(self, mhc_parser: MhcParser): self.mhc_parser = mhc_parser From 69d417e797a1a36d55995e91b9ae7143b93a69a6 Mon Sep 17 00:00:00 2001 From: nguyenhv Date: Wed, 8 Nov 2023 15:44:32 +0100 Subject: [PATCH 06/66] remove R dependency --- MANIFEST.in | 1 - docs/source/02_installation.md | 34 ++----------------- docs/source/03_03_usage.md | 1 - docs/source/notebooks/api_usage.ipynb | 1 - neofox/__init__.py | 3 +- neofox/command_line.py | 10 ++---- neofox/references/installer.py | 18 ++-------- neofox/references/references.py | 3 -- .../tests/integration_tests/test_installer.py | 2 +- neofox/tests/resources/neofox_config.txt | 1 - .../test_dependencies_configuration.py | 2 -- 11 files changed, 9 insertions(+), 67 deletions(-) diff --git a/MANIFEST.in b/MANIFEST.in index c010435d..988c5cb8 100755 --- a/MANIFEST.in +++ b/MANIFEST.in @@ -3,7 +3,6 @@ include neofox/published_features/Tcell_predictor/amino-acids-features.pickle include neofox/published_features/Tcell_predictor/genes-expression.pickle include neofox/published_features/Tcell_predictor/SIRdata.mat include neofox/published_features/Tcell_predictor/Classifier.pickle -include neofox/references/install_r_dependencies.R include neofox/references/h2_database_allele_list.csv include neofox/expression_imputation/tcga_cohort_code.tab include neofox/expression_imputation/tcga_exp_summary_modified.tab.gz.tbi diff --git a/docs/source/02_installation.md b/docs/source/02_installation.md index 4828ede5..0c84062c 100755 --- a/docs/source/02_installation.md +++ b/docs/source/02_installation.md @@ -58,16 +58,6 @@ conda install bioconda::neofox **NOTE**: Please, check the licences of third-party dependencies. -#### Install R - -R 3.6.0 is required. - -Optionally set the environment variable pointing to `Rscript`, otherwise neofox will look for it in the path. -``` -export NEOFOX_RSCRIPT=`which Rscript` -``` - -**NOTE**: when installing from conda this dependency is already installed. #### Install BLASTP @@ -172,12 +162,11 @@ Configure PRIME as explained in the file `PRIME-master/README` ### Configuration of the reference folder -To configure the reference folder, set the environment variables for `makeblastdb`, NetMHCpan, NetMHCIIpan and Rscript, +To configure the reference folder, set the environment variables for `makeblastdb`, NetMHCpan, and NetMHCIIpan, or alternatively rely on these being fetched from the path: ``` export NEOFOX_MAKEBLASTDB=`pwd`/ncbi-blast-2.10.1+/bin/makeblastdb -export NEOFOX_RSCRIPT=`which Rscript` export NEOFOX_NETMHCPAN=`pwd`/netMHCpan-4.1/netMHCpan export NEOFOX_NETMHC2PAN=`pwd`/netMHCIIpan-4.0/netMHCIIpan ``` @@ -194,7 +183,6 @@ Run the following to configure the NeoFox reference folder: neofox-configure --reference-folder /your/neofox/folder [--install-r-dependencies --install_mouse_mixmhc2pred] ``` -**NOTE**: when installing from conda `--install-r-dependencies` is not needed. The above command will download and transform several resources and store in the annotations metadata their version, MD5 checksum and download timestamp. @@ -204,16 +192,7 @@ To run NeoFox on data from mouse with MixMHC2pred, mouse-specific PMWs are requi Depending on your use case please check the licences of these third-party resources (see urls in neofox/references/installer.py). -Unless indicated to the installer by flag `--install-r-dependencies` you will need to install manually some R packages. These packages are the following: -``` -lattice -ggplot2 -caret -Peptides -doParallel -gbm -Biostrings -``` + Add the reference folder to the Path ``` @@ -229,11 +208,4 @@ The test data can be downloaded here: * [test_patients.tsv](_static/test_patients.tsv) ````commandline -neofox --input-file /path/to/test_data.txt --patient-data /path/to/test_patients.txt --output-folder /path/to/outputfolder --output-prefix test -```` - -The resulting output files can be compared to the following test output files: - -* [test_neoantigen_candidates_annotated.tsv](_static/test_neoantigen_candidates_annotated.tsv) -* [test_neoantigen_candidates_annotated.json](_static/test_neoantigen_candidates_annotated.json) - +neofox --input-file /path/to/test_data.tx \ No newline at end of file diff --git a/docs/source/03_03_usage.md b/docs/source/03_03_usage.md index 6f4a4a4a..a553f764 100755 --- a/docs/source/03_03_usage.md +++ b/docs/source/03_03_usage.md @@ -53,7 +53,6 @@ neofox --input-file neoantigens_candidates.tsv \ The optional **config** file with the paths to the dependencies can look like this: ````commandline NEOFOX_REFERENCE_FOLDER=path/to/reference/folder -NEOFOX_RSCRIPT=`which Rscript` NEOFOX_BLASTP=path/to/blast/bin/blastp NEOFOX_NETMHCPAN=path/to/netMHCpan/netMHCpan NEOFOX_NETMHC2PAN=path/to/netMHCIIpan/netMHCIIpan diff --git a/docs/source/notebooks/api_usage.ipynb b/docs/source/notebooks/api_usage.ipynb index 3b34feda..e2c593fa 100644 --- a/docs/source/notebooks/api_usage.ipynb +++ b/docs/source/notebooks/api_usage.ipynb @@ -1701,7 +1701,6 @@ ], "source": [ "os.environ[\"NEOFOX_REFERENCE_FOLDER\"] = \"/neofox_install/reference_data/\"\n", - "os.environ[\"NEOFOX_RSCRIPT\"] = \"/usr/bin/Rscript\"\n", "os.environ[\"NEOFOX_BLASTP\"] = \"/neofox_install/ncbi-blast-2.10.1+/bin/blastp\"\n", "os.environ[\"NEOFOX_NETMHCPAN\"] = \"/neofox_install/netMHCpan-4.1/netMHCpan\"\n", "os.environ[\"NEOFOX_NETMHC2PAN\"] = \"/neofox_install/netMHCIIpan-4.0/netMHCIIpan\"\n", diff --git a/neofox/__init__.py b/neofox/__init__.py index b86ecb14..33440f3f 100755 --- a/neofox/__init__.py +++ b/neofox/__init__.py @@ -18,13 +18,12 @@ # along with this program. If not, see .# -VERSION = "1.1.1.dev2" +VERSION = "1.1.1.dev3" REFERENCE_FOLDER_ENV = "NEOFOX_REFERENCE_FOLDER" NEOFOX_BLASTP_ENV = "NEOFOX_BLASTP" NEOFOX_MIXMHC2PRED_ENV = "NEOFOX_MIXMHC2PRED" NEOFOX_MIXMHCPRED_ENV = "NEOFOX_MIXMHCPRED" -NEOFOX_RSCRIPT_ENV = "NEOFOX_RSCRIPT" NEOFOX_NETMHC2PAN_ENV = "NEOFOX_NETMHC2PAN" NEOFOX_NETMHCPAN_ENV = "NEOFOX_NETMHCPAN" NEOFOX_MAKEBLASTDB_ENV = "NEOFOX_MAKEBLASTDB" diff --git a/neofox/command_line.py b/neofox/command_line.py index f18f6665..3e024c4b 100755 --- a/neofox/command_line.py +++ b/neofox/command_line.py @@ -45,12 +45,7 @@ def neofox_configure(): help="the folder with the references required for Neofox", required=True, ) - parser.add_argument( - "--install-r-dependencies", - dest="install_r_dependencies", - action="store_true", - help="install the R dependencies automatically", - ) + parser.add_argument( "--install-mouse-mixmhc2pred", dest="install_mouse_mixmhc2pred", @@ -60,7 +55,6 @@ def neofox_configure(): args = parser.parse_args() reference_folder = args.reference_folder - install_r_dependencies = args.install_r_dependencies install_mouse_mixmhc2pred = args.install_mouse_mixmhc2pred # makes sure that the output folder exists @@ -68,7 +62,7 @@ def neofox_configure(): logger.info("Starting the installation of references") NeofoxReferenceInstaller( - reference_folder=reference_folder, install_r_dependencies=install_r_dependencies, + reference_folder=reference_folder, install_mouse_mixmhc2pred=install_mouse_mixmhc2pred ).install() logger.info("Finished the installation succesfully!") diff --git a/neofox/references/installer.py b/neofox/references/installer.py index 77a90c87..972e4714 100644 --- a/neofox/references/installer.py +++ b/neofox/references/installer.py @@ -46,11 +46,10 @@ class NeofoxReferenceInstaller(object): - def __init__(self, reference_folder, install_r_dependencies=False, install_mouse_mixmhc2pred=False): + def __init__(self, reference_folder, install_mouse_mixmhc2pred=False): self.config = DependenciesConfigurationForInstaller() self.runner = Runner() self.reference_folder = reference_folder - self.install_r_dependencies = install_r_dependencies self.install_mouse_mixmhc2pred = install_mouse_mixmhc2pred def install(self): @@ -65,10 +64,7 @@ def install(self): proteome_resources = self._set_proteome() hla_resource = self._set_ipd_imgt_hla_database() self._set_h2_resource() - if self.install_r_dependencies: - self._install_r_dependencies() - else: - logger.warning("R dependencies will need to be installed manually") + mixmhc2pred_resources = [] if self.install_mouse_mixmhc2pred: mixmhc2pred_resources = self._set_mixmhc2pred_pwms() @@ -347,16 +343,6 @@ def _set_h2_resource(self): target_file = os.path.join(self.reference_folder, H2_DATABASE_AVAILABLE_ALLELES_FILE) copyfile(source_file, target_file) - def _install_r_dependencies(self): - logger.info("Installing R dependencies...") - cmd = "{rscript} {dependencies_file}".format( - rscript=self.config.rscript, - dependencies_file=os.path.join( - os.path.abspath(os.path.dirname(__file__)), "install_r_dependencies.R" - ), - ) - self._run_command(cmd) - def _set_mixmhc2pred_pwms(self): # Downloads PWMs of other species than human from http://mixmhc2pred.gfellerlab.org/PWMdef # Currently only mouse is supported and downloaded diff --git a/neofox/references/references.py b/neofox/references/references.py index 20922716..c46b4e1c 100755 --- a/neofox/references/references.py +++ b/neofox/references/references.py @@ -32,7 +32,6 @@ DEFAULT_PRIME = 'PRIME' DEFAULT_NETMHCPAN = 'netMHCpan' DEFAULT_NETMHC2PAN = 'netMHCIIpan' -DEFAULT_RSCRIPT = 'Rscript' DEFAULT_MIXMHCPRED = "MixMHCpred" DEFAULT_MIXMHC2PRED = "MixMHC2pred_unix" DEFAULT_BLASTP = "blastp" @@ -146,7 +145,6 @@ def __init__(self): os.path.dirname(self.mix_mhc_pred), "lib", MIXMHCPRED_AVAILABLE_ALLELES_FILE) else: self.mix_mhc_pred_alleles_list = None - self.rscript = self._check_and_load_binary(neofox.NEOFOX_RSCRIPT_ENV, default_value=DEFAULT_RSCRIPT) self.net_mhc2_pan = self._check_and_load_binary(neofox.NEOFOX_NETMHC2PAN_ENV, default_value=DEFAULT_NETMHC2PAN) self.net_mhc_pan = self._check_and_load_binary(neofox.NEOFOX_NETMHCPAN_ENV, default_value=DEFAULT_NETMHCPAN) self.prime = self._check_and_load_binary(neofox.NEOFOX_PRIME_ENV, default_value=DEFAULT_PRIME, optional=True, @@ -162,7 +160,6 @@ def __init__(self): self.net_mhc2_pan = self._check_and_load_binary(neofox.NEOFOX_NETMHC2PAN_ENV, default_value=DEFAULT_NETMHC2PAN) self.net_mhc_pan = self._check_and_load_binary(neofox.NEOFOX_NETMHCPAN_ENV, default_value=DEFAULT_NETMHCPAN) self.make_blastdb = self._check_and_load_binary(neofox.NEOFOX_MAKEBLASTDB_ENV, default_value=DEFAULT_MAKEBLASTDB) - self.rscript = self._check_and_load_binary(neofox.NEOFOX_RSCRIPT_ENV, default_value=DEFAULT_RSCRIPT) class MhcDatabase(ABC): diff --git a/neofox/tests/integration_tests/test_installer.py b/neofox/tests/integration_tests/test_installer.py index f1d0c862..09d1e98a 100644 --- a/neofox/tests/integration_tests/test_installer.py +++ b/neofox/tests/integration_tests/test_installer.py @@ -17,5 +17,5 @@ def test_installer(self): neofox.tests.__name__, "resources/test_installation_{:%Y%m%d%H%M%S}".format(datetime.now())) NeofoxReferenceInstaller( - reference_folder=reference_folder, install_r_dependencies=False + reference_folder=reference_folder ).install() diff --git a/neofox/tests/resources/neofox_config.txt b/neofox/tests/resources/neofox_config.txt index 7b3a28c8..aa0f2feb 100644 --- a/neofox/tests/resources/neofox_config.txt +++ b/neofox/tests/resources/neofox_config.txt @@ -1,4 +1,3 @@ -NEOFOX_RSCRIPT=/neofox/testing/Rscript NEOFOX_BLASTP=/neofox/testing/ncbi-blast-2.10.1+/bin/blastp NEOFOX_NETMHCPAN=/neofox/testing/netMHCpan-4.0/netMHCpan NEOFOX_NETMHC2PAN=/neofox/testing/netMHCIIpan-3.2/netMHCIIpan diff --git a/neofox/tests/unit_tests/test_dependencies_configuration.py b/neofox/tests/unit_tests/test_dependencies_configuration.py index 3f784d72..02ddfdab 100755 --- a/neofox/tests/unit_tests/test_dependencies_configuration.py +++ b/neofox/tests/unit_tests/test_dependencies_configuration.py @@ -32,7 +32,6 @@ def setUp(self): neofox.NEOFOX_BLASTP_ENV: "/path/to/blastp", neofox.NEOFOX_NETMHC2PAN_ENV: "/path/to/netmhc2pan", neofox.NEOFOX_NETMHCPAN_ENV: "/path/to/netmhcpan", - neofox.NEOFOX_RSCRIPT_ENV: "/path/to/rscript", neofox.NEOFOX_MIXMHCPRED_ENV: "/path/to/mixmhcpred", neofox.NEOFOX_MIXMHC2PRED_ENV: "/path/to/mixmhc2pred", neofox.NEOFOX_PRIME_ENV: "/path/to/prime", @@ -94,7 +93,6 @@ def test_all_resources_exist(self): self.assertTrue( config.mix_mhc_pred == self.variables[neofox.NEOFOX_MIXMHCPRED_ENV] ) - self.assertTrue(config.rscript == self.variables[neofox.NEOFOX_RSCRIPT_ENV]) self.assertTrue( config.net_mhc_pan == self.variables[neofox.NEOFOX_NETMHCPAN_ENV] ) From c5e668145974f09217bc54495145f8722d64ccfb Mon Sep 17 00:00:00 2001 From: nguyenhv Date: Wed, 8 Nov 2023 19:06:36 +0100 Subject: [PATCH 07/66] fix copying errors --- docs/source/02_installation.md | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/docs/source/02_installation.md b/docs/source/02_installation.md index 0c84062c..c931784a 100755 --- a/docs/source/02_installation.md +++ b/docs/source/02_installation.md @@ -208,4 +208,5 @@ The test data can be downloaded here: * [test_patients.tsv](_static/test_patients.tsv) ````commandline -neofox --input-file /path/to/test_data.tx \ No newline at end of file +neofox --input-file /path/to/test_data.txt --patient-data /path/to/test_patients.txt --output-folder /path/to/outputfolder --output-prefix test +```` \ No newline at end of file From 9008376370f317641e7cbb67931025fd1bb1f3b4 Mon Sep 17 00:00:00 2001 From: nguyenhv Date: Wed, 8 Nov 2023 19:17:28 +0100 Subject: [PATCH 08/66] add accidentally deleted info --- docs/source/02_installation.md | 7 ++++++- 1 file changed, 6 insertions(+), 1 deletion(-) diff --git a/docs/source/02_installation.md b/docs/source/02_installation.md index c931784a..84aa5cbe 100755 --- a/docs/source/02_installation.md +++ b/docs/source/02_installation.md @@ -209,4 +209,9 @@ The test data can be downloaded here: ````commandline neofox --input-file /path/to/test_data.txt --patient-data /path/to/test_patients.txt --output-folder /path/to/outputfolder --output-prefix test -```` \ No newline at end of file +```` + +The resulting output files can be compared to the following test output files: + +* [test_neoantigen_candidates_annotated.tsv](_static/test_neoantigen_candidates_annotated.tsv) +* [test_neoantigen_candidates_annotated.json](_static/test_neoantigen_candidates_annotated.json) \ No newline at end of file From cf23ed311238a42bd6e2d21e63363aa7e693db26 Mon Sep 17 00:00:00 2001 From: nguyenhv Date: Wed, 8 Nov 2023 19:51:10 +0100 Subject: [PATCH 09/66] update docu --- README.md | 4 +--- docs/source/02_installation.md | 2 +- 2 files changed, 2 insertions(+), 4 deletions(-) diff --git a/README.md b/README.md index dae32e1d..7181c980 100755 --- a/README.md +++ b/README.md @@ -52,7 +52,6 @@ NeoFox covers the following neoantigen features and prediction algorithms: NeoFox depends on the following tools: - Python >=3.7, <=3.8 -- R 3.6.0 - BLAST 2.10.1 - netMHCpan 4.1 - netMHCIIpan 4.0 @@ -96,7 +95,6 @@ neofox --candidate-file/--json-file neoantigens_candidates.tab/neoantigens_candi The optional config file with the paths to the dependencies can look like this: ````commandline NEOFOX_REFERENCE_FOLDER=path/to/reference/folder -NEOFOX_RSCRIPT=`which Rscript` NEOFOX_BLASTP=path/to/ncbi-blast-2.10.1+/bin/blastp NEOFOX_NETMHCPAN=path/to/netMHCpan-4.1/netMHCpan NEOFOX_NETMHC2PAN=path/to/netMHCIIpan-4.0/netMHCIIpan @@ -163,4 +161,4 @@ where: ## 5 Output data -The output data is returned by default in a short wide tab separated values file (`--with-table`). Optionally, it can be provided in JSON format (`--with-json`). +The output data is returned by default in a short wide tab separated values file (`--with-table`). Optionally, it can be provided in JSON format (`--with-json`). \ No newline at end of file diff --git a/docs/source/02_installation.md b/docs/source/02_installation.md index 84aa5cbe..a3e7b57a 100755 --- a/docs/source/02_installation.md +++ b/docs/source/02_installation.md @@ -180,7 +180,7 @@ export NEOFOX_HLA_DATABASE=https://raw.githubusercontent.com/ANHIG/IMGTHLA/Lates Run the following to configure the NeoFox reference folder: ``` -neofox-configure --reference-folder /your/neofox/folder [--install-r-dependencies --install_mouse_mixmhc2pred] +neofox-configure --reference-folder /your/neofox/folder [--install_mouse_mixmhc2pred] ``` From c5f3c61e71aadc835042e1bc59ca7f1bfcc55a15 Mon Sep 17 00:00:00 2001 From: nguyenhv Date: Thu, 23 Nov 2023 13:39:20 +0100 Subject: [PATCH 10/66] meaningful error messages if the separator is not supported --- neofox/__init__.py | 2 +- neofox/annotator/neoantigen_annotator.py | 4 +- neofox/model/conversion.py | 51 ++++++++++++++---------- 3 files changed, 32 insertions(+), 25 deletions(-) diff --git a/neofox/__init__.py b/neofox/__init__.py index 33440f3f..3bf714be 100755 --- a/neofox/__init__.py +++ b/neofox/__init__.py @@ -18,7 +18,7 @@ # along with this program. If not, see .# -VERSION = "1.1.1.dev3" +VERSION = "1.1.1.dev4" REFERENCE_FOLDER_ENV = "NEOFOX_REFERENCE_FOLDER" NEOFOX_BLASTP_ENV = "NEOFOX_BLASTP" diff --git a/neofox/annotator/neoantigen_annotator.py b/neofox/annotator/neoantigen_annotator.py index fd2d6d86..453ec609 100755 --- a/neofox/annotator/neoantigen_annotator.py +++ b/neofox/annotator/neoantigen_annotator.py @@ -26,14 +26,12 @@ from neofox.MHC_predictors.prime import Prime from neofox.annotator.abstract_annotator import AbstractAnnotator from neofox.annotator.neoantigen_mhc_binding_annotator import NeoantigenMhcBindingAnnotator -from neofox.helpers.epitope_helper import EpitopeHelper -from neofox.MHC_predictors.netmhcpan.combine_netmhcpan_pred_multiple_binders import BestAndMultipleBinder from neofox.model.factories import AnnotationFactory from neofox.model.mhc_parser import MhcParser from neofox.published_features.Tcell_predictor.tcellpredictor_wrapper import TcellPrediction from neofox.published_features.self_similarity.self_similarity import SelfSimilarityCalculator from neofox.published_features.expression import Expression -from neofox.model.neoantigen import Patient, Neoantigen, Annotations, PredictedEpitope +from neofox.model.neoantigen import Patient, Neoantigen, Annotations from neofox.published_features.vaxrank.vaxrank import VaxRank from neofox.references.references import ( ReferenceFolder, diff --git a/neofox/model/conversion.py b/neofox/model/conversion.py index 0eec8eb0..4f6399b3 100755 --- a/neofox/model/conversion.py +++ b/neofox/model/conversion.py @@ -37,7 +37,7 @@ NeoepitopeFactory, ) from neofox.references.references import MhcDatabase - +from logzero import logger class ModelConverter(object): @@ -47,6 +47,7 @@ def parse_candidate_file(candidate_file: str) -> List[Neoantigen]: :param candidate_file: the path to an neoantigen candidate input file :return neoantigens in model objects """ + data = pd.read_csv( candidate_file, sep="\t", # NOTE: forces the types of every column to avoid pandas setting the wrong type for corner cases @@ -60,12 +61,15 @@ def parse_candidate_file(candidate_file: str) -> List[Neoantigen]: "rnaVariantAlleleFrequency": float } ) - + # there is a limitation here + # It could be a data has more than one type of separators due to human mistakes + if data.shape[1] == 1: + logger.fatal('Input data has an unsupported delimiter in NeoFox. NeoFox supports only the tab delimiter.') # NOTE: this is the support for the NeoFox format - data = data.replace({np.nan: None}) - neoantigens = ModelConverter._neoantigens_csv2objects(data) - - return neoantigens + else: + data = data.replace({np.nan: None}) + neoantigens = ModelConverter._neoantigens_csv2objects(data) + return neoantigens @staticmethod def parse_candidate_neoepitopes_file(candidate_file: str, mhc_database: MhcDatabase, organism: str) -> List[PredictedEpitope]: @@ -84,11 +88,13 @@ def parse_candidate_neoepitopes_file(candidate_file: str, mhc_database: MhcDatab "isoformMhcII": str, } ) - + if data.shape[1] == 1: + logger.fatal('Input data has an unsupported delimiter in NeoFox. NeoFox supports only the tab delimiter.') # NOTE: this is the support for the NeoFox format - data = data.replace({np.nan: None}) - neoepitopes = ModelConverter._neoepitopes_csv2objects(data, mhc_database, organism) - return neoepitopes + else: + data = data.replace({np.nan: None}) + neoepitopes = ModelConverter._neoepitopes_csv2objects(data, mhc_database, organism) + return neoepitopes @staticmethod @@ -110,18 +116,21 @@ def parse_patients_file(patients_file: str, mhc_database: MhcDatabase) -> List[P "identifier": str } ) - patients = [] - for _, row in df.iterrows(): - patient_dict = row.to_dict() - patient = PatientFactory.build_patient( - identifier=patient_dict.get("identifier"), - tumor_type=patient_dict.get("tumorType"), - mhc_alleles=patient_dict.get("mhcIAlleles", []), - mhc2_alleles=patient_dict.get("mhcIIAlleles", []), - mhc_database=mhc_database - ) - patients.append(patient) + + if df.shape[1] == 1: + logger.fatal('The patient data has an unsupported delimiter in NeoFox. NeoFox supports only the tab delimiter.') + else: + for _, row in df.iterrows(): + patient_dict = row.to_dict() + patient = PatientFactory.build_patient( + identifier=patient_dict.get("identifier"), + tumor_type=patient_dict.get("tumorType"), + mhc_alleles=patient_dict.get("mhcIAlleles", []), + mhc2_alleles=patient_dict.get("mhcIIAlleles", []), + mhc_database=mhc_database + ) + patients.append(patient) return patients @staticmethod From fc1cfc962148be46e11fe6c3f497855542270b57 Mon Sep 17 00:00:00 2001 From: nguyenhv Date: Thu, 23 Nov 2023 14:54:36 +0100 Subject: [PATCH 11/66] add message into log-file that PRIME, MixMHCpred or MixMHC2pred were run --- neofox/__init__.py | 2 +- neofox/annotator/neoantigen_annotator.py | 1 - neofox/command_line.py | 5 +++++ 3 files changed, 6 insertions(+), 2 deletions(-) diff --git a/neofox/__init__.py b/neofox/__init__.py index 3bf714be..06420eba 100755 --- a/neofox/__init__.py +++ b/neofox/__init__.py @@ -18,7 +18,7 @@ # along with this program. If not, see .# -VERSION = "1.1.1.dev4" +VERSION = "1.1.1.dev5" REFERENCE_FOLDER_ENV = "NEOFOX_REFERENCE_FOLDER" NEOFOX_BLASTP_ENV = "NEOFOX_BLASTP" diff --git a/neofox/annotator/neoantigen_annotator.py b/neofox/annotator/neoantigen_annotator.py index 453ec609..398cc8fc 100755 --- a/neofox/annotator/neoantigen_annotator.py +++ b/neofox/annotator/neoantigen_annotator.py @@ -18,7 +18,6 @@ # You should have received a copy of the GNU General Public License # along with this program. If not, see .# -from logzero import logger from datetime import datetime import neofox from neofox.MHC_predictors.MixMHCpred.mixmhc2pred import MixMHC2pred diff --git a/neofox/command_line.py b/neofox/command_line.py index 3e024c4b..8da6af8e 100755 --- a/neofox/command_line.py +++ b/neofox/command_line.py @@ -166,6 +166,11 @@ def neofox_cli(): dotenv.load_dotenv(config, override=True) reference_folder = ReferenceFolder(organism=organism) + if organism=='human': + logger.info('PRIME, MixMHCpred and MixMHC2pred were run.') + else: + logger.info('MixMHC2pred was run.') + # reads the input data neoantigens, patients = _read_data( input_file, From a927149596c93ffcccfc1563306e8ea1a3b52171 Mon Sep 17 00:00:00 2001 From: nguyenhv Date: Thu, 23 Nov 2023 16:28:53 +0100 Subject: [PATCH 12/66] change the tense in the sentences --- neofox/command_line.py | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/neofox/command_line.py b/neofox/command_line.py index 8da6af8e..47528272 100755 --- a/neofox/command_line.py +++ b/neofox/command_line.py @@ -167,9 +167,9 @@ def neofox_cli(): reference_folder = ReferenceFolder(organism=organism) if organism=='human': - logger.info('PRIME, MixMHCpred and MixMHC2pred were run.') + logger.info('PRIME, MixMHCpred and MixMHC2pred will be run.') else: - logger.info('MixMHC2pred was run.') + logger.info('MixMHC2pred will be run.') # reads the input data neoantigens, patients = _read_data( From d13a27dc510efd1b6cec862ea19f0e66f64dbfb1 Mon Sep 17 00:00:00 2001 From: nguyenhv Date: Thu, 23 Nov 2023 16:59:48 +0100 Subject: [PATCH 13/66] edit documentation for supported separator --- docs/source/03_01_input_data.md | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/docs/source/03_01_input_data.md b/docs/source/03_01_input_data.md index 9457da92..5ab3362a 100644 --- a/docs/source/03_01_input_data.md +++ b/docs/source/03_01_input_data.md @@ -2,7 +2,8 @@ ## General information -NeoFox requires two input files: a candidate file with neoantigen or neoepitope candidates and a file with patient data. +NeoFox requires two input files: a candidate file with neoantigen or neoepitope candidates and a file with patient data. +The values between columns in both input files must be tab-separated. The file with neoantigen candidates can be provided either in tabular format or in JSON format and this file may contain additional user-specific input that will be kept during the annotation process. The patient file requires a tabular format. From 4337755cd8f787aed325bf5bb097e93317e15abb Mon Sep 17 00:00:00 2001 From: nguyenhv Date: Thu, 23 Nov 2023 17:06:13 +0100 Subject: [PATCH 14/66] edit documentation --- docs/source/03_01_input_data.md | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/docs/source/03_01_input_data.md b/docs/source/03_01_input_data.md index 5ab3362a..69ce881b 100644 --- a/docs/source/03_01_input_data.md +++ b/docs/source/03_01_input_data.md @@ -3,8 +3,7 @@ ## General information NeoFox requires two input files: a candidate file with neoantigen or neoepitope candidates and a file with patient data. -The values between columns in both input files must be tab-separated. -The file with neoantigen candidates can be provided either in tabular format or in JSON format and this file may contain +Both the input files must be tab-separated. The file with neoantigen candidates can be provided either in tabular format or in JSON format and this file may contain additional user-specific input that will be kept during the annotation process. The patient file requires a tabular format. Alternatively, NeoFox may annotate a set of neoepitope candidates for which it will require a file with neoepitope From a3fe9430d6967b4fc17b63b91273937f9b0f6151 Mon Sep 17 00:00:00 2001 From: nguyenhv Date: Thu, 7 Dec 2023 11:38:52 +0100 Subject: [PATCH 15/66] add log information about CPUs and organims --- neofox/command_line.py | 4 ++++ neofox/neofox.py | 3 ++- 2 files changed, 6 insertions(+), 1 deletion(-) diff --git a/neofox/command_line.py b/neofox/command_line.py index 47528272..119c2c29 100755 --- a/neofox/command_line.py +++ b/neofox/command_line.py @@ -167,10 +167,14 @@ def neofox_cli(): reference_folder = ReferenceFolder(organism=organism) if organism=='human': + logger.info('Organism is human.') logger.info('PRIME, MixMHCpred and MixMHC2pred will be run.') else: + logger.info('Organism is mouse.') logger.info('MixMHC2pred will be run.') + logger.info('Number of CPUs being used: {}'.format(num_cpus)) + # reads the input data neoantigens, patients = _read_data( input_file, diff --git a/neofox/neofox.py b/neofox/neofox.py index 0c8b0761..644a715f 100755 --- a/neofox/neofox.py +++ b/neofox/neofox.py @@ -113,7 +113,8 @@ def __init__( self.neoantigens = self._conditional_expression_imputation() self.with_all_neoepitopes = with_all_neoepitopes - + if with_all_neoepitopes: + logger.info("Prediction of all neoepitopes will be performed") logger.info("Reference data loaded") def _conditional_expression_imputation(self) -> List[Neoantigen]: From db95633900ebaac717ecf99d26d5279fcae853c9 Mon Sep 17 00:00:00 2001 From: nguyenhv Date: Thu, 7 Dec 2023 13:41:46 +0100 Subject: [PATCH 16/66] add information into log files about supported alleles --- .../MHC_predictors/MixMHCpred/mixmhcpred.py | 2 +- neofox/MHC_predictors/prime.py | 2 +- neofox/helpers/mhc_helper.py | 20 ++++++++++++------- 3 files changed, 15 insertions(+), 9 deletions(-) diff --git a/neofox/MHC_predictors/MixMHCpred/mixmhcpred.py b/neofox/MHC_predictors/MixMHCpred/mixmhcpred.py index a4c85fcd..7ca163b8 100755 --- a/neofox/MHC_predictors/MixMHCpred/mixmhcpred.py +++ b/neofox/MHC_predictors/MixMHCpred/mixmhcpred.py @@ -80,7 +80,7 @@ def run(self, neoantigen: Neoantigen, mhc: List[Mhc1], uniprot): ) if len(potential_ligand_sequences) > 0: mhc1_alleles = self.parsed_mhc_alleles.get_mixmhc_allele_representation(self.configuration.mix_mhc_pred_alleles_list, - [a for m in mhc for a in m.alleles]) + [a for m in mhc for a in m.alleles], 'MixMHCpred') if len(mhc1_alleles) > 0: self.results = self._mixmhcprediction(mhc1_alleles, potential_ligand_sequences) else: diff --git a/neofox/MHC_predictors/prime.py b/neofox/MHC_predictors/prime.py index 9bc26327..6c64c39f 100755 --- a/neofox/MHC_predictors/prime.py +++ b/neofox/MHC_predictors/prime.py @@ -89,7 +89,7 @@ def run(self, neoantigen: Neoantigen, mhc: List[Mhc1], uniprot): ) if len(potential_ligand_sequences) > 0: mhc1_alleles = self.parsed_mhc_alleles.get_mixmhc_allele_representation(self.configuration.prime_alleles_list, - [a for m in mhc for a in m.alleles]) + [a for m in mhc for a in m.alleles], 'PRIME') if len(mhc1_alleles) > 0: self.results = self._prime(mhc1_alleles, potential_ligand_sequences) else: diff --git a/neofox/helpers/mhc_helper.py b/neofox/helpers/mhc_helper.py index b4a9e13e..20cfe677 100644 --- a/neofox/helpers/mhc_helper.py +++ b/neofox/helpers/mhc_helper.py @@ -40,22 +40,28 @@ def __init__(self, mhc_parser: MhcParser): self.mhc_parser = mhc_parser @staticmethod - def get_mixmhc_allele_representation(file_alleles, mhc_alleles: List[MhcAllele]): + def get_mixmhc_allele_representation(file_alleles, mhc_alleles: List[MhcAllele], prime_mixmhcpred): """ - loads file with available HLA II alllels for MixMHC2pred prediction, returns set + loads file with available HLA II alleles for MixMHCpred and PRIME prediction, returns set :return: """ alleles = pd.read_csv( file_alleles, sep="\t" ) available_alleles = set(alleles["Allele"]) - return list( - filter( - lambda y: y in available_alleles, - map( + + converted_mhc_alleles = list(map( lambda x: "{gene}{group}{protein}".format(gene=x.gene, group=x.group, protein=x.protein), - mhc_alleles) + mhc_alleles)) + + not_available_alleles = list(set(converted_mhc_alleles).difference(available_alleles)) + if len(not_available_alleles) > 0: + logger.warning( + "MHC I alleles {} are not supported by {}.".format(",".join(not_available_alleles), prime_mixmhcpred) ) + + return list( + set(converted_mhc_alleles).intersection(available_alleles) ) def parse_mixmhcpred_prime_output(self, mixmhc_prime_result) -> List[PredictedEpitope]: From 61ced6189354932c850705c0691a4ad9f72c44dc Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Thu, 4 Jan 2024 17:07:15 +0100 Subject: [PATCH 17/66] Improve user experience: * Added --verbose flag to README and docs * Changed logging to show the version by default and show information on the peptide and tool in verbose mode * Added the supported peptide length to the error message in validation.py --- README.md | 1 + docs/source/03_03_usage.md | 6 ++- .../annotation_resources/uniprot/uniprot.py | 4 +- neofox/command_line.py | 31 ++++++++++---- neofox/helpers/runner.py | 6 +-- neofox/model/validation.py | 42 +++++++++++-------- neofox/neofox.py | 24 ++++++----- neofox/neofox_epitope.py | 16 +++---- .../neoantigen_fitness/neoantigen_fitness.py | 2 +- 9 files changed, 82 insertions(+), 50 deletions(-) diff --git a/README.md b/README.md index 7181c980..de98e184 100755 --- a/README.md +++ b/README.md @@ -90,6 +90,7 @@ neofox --candidate-file/--json-file neoantigens_candidates.tab/neoantigens_candi - `--organism`: the organism to which the data corresponds. Possible values: [human, mouse]. Default value: human - `--affinity-threshold`: a affinity value (*optional*) neoantigen candidates with a best predicted affinity greater than or equal than this threshold will be not annotated with features that specifically model neoepitope recognition. A threshold that is commonly used is 500 nM. +- `--verbose`: get detailed logs The optional config file with the paths to the dependencies can look like this: diff --git a/docs/source/03_03_usage.md b/docs/source/03_03_usage.md index a553f764..6921fab5 100755 --- a/docs/source/03_03_usage.md +++ b/docs/source/03_03_usage.md @@ -19,7 +19,8 @@ neofox --input-file neoantigens_candidates.tsv \ [--num-cpus] \ [--config] \ [--patient-id] \ - [--with-all-neoepitopes] + [--with-all-neoepitopes] \ + [--verbose] ```` where: @@ -36,6 +37,7 @@ where: - `--num-cpus`: number of CPUs to use (*optional*) - `--config`: a config file with the paths to dependencies as shown below (*optional*) - `--patient-id`: patient identifier (*optional*, this is only relevant if the column `patientIdentifier` is missing in the candidate input file) +- `--verbose`: get detailed logs **PLEASE NOTE THE FOLLOWING HINTS**: - If a tumor type has been provided in the patient file, imputated gene expression from TCGA will be annoated and expression-dependent features will be determined with imputated gene expression aswell. @@ -74,6 +76,7 @@ neofox-epitope --input-file neoepitope_candidates.tsv \ [--organism human|mouse] \ [--num-cpus] \ [--config] \ + [--verbose] ```` where: @@ -85,6 +88,7 @@ where: - `--organism`: the organism to which the data corresponds. Possible values: [human, mouse]. Default value: human - `--num-cpus`: number of CPUs to use (*optional*) - `--config`: a config file with the paths to dependencies as shown below (*optional*) +- `--verbose`: get detailed logs ## Running from docker diff --git a/neofox/annotation_resources/uniprot/uniprot.py b/neofox/annotation_resources/uniprot/uniprot.py index ec81978b..7668326f 100755 --- a/neofox/annotation_resources/uniprot/uniprot.py +++ b/neofox/annotation_resources/uniprot/uniprot.py @@ -31,9 +31,9 @@ class Uniprot(object): """ def __init__(self, proteome): - logger.info("Loading Uniprot...") + logger.debug("Loading Uniprot...") self.uniprot = self._load_proteome(proteome) - logger.info("Loaded Uniprot.") + logger.debug("Loaded Uniprot.") @staticmethod def _load_proteome(proteome) -> str: diff --git a/neofox/command_line.py b/neofox/command_line.py index 119c2c29..51222a6f 100755 --- a/neofox/command_line.py +++ b/neofox/command_line.py @@ -33,7 +33,7 @@ from neofox.references.references import ReferenceFolder, ORGANISM_HOMO_SAPIENS, ORGANISM_MUS_MUSCULUS, MhcDatabase epilog = "NeoFox (NEOantigen Feature toolbOX) {}. Copyright (c) 2020-2021 " \ - "TRON – Translational Oncology at the University Medical Center of the " \ + "TRON - Translational Oncology at the University Medical Center of the " \ "Johannes Gutenberg University Mainz gGmbH, all rights reserved".format(neofox.VERSION) @@ -190,7 +190,8 @@ def neofox_cli(): reference_folder=reference_folder, rank_mhci_threshold=rank_mhci_threshold, rank_mhcii_threshold=rank_mhcii_threshold, - with_all_neoepitopes=with_all_neoepitopes + with_all_neoepitopes=with_all_neoepitopes, + verbose=args.verbose ).get_annotations() _write_results( @@ -208,11 +209,19 @@ def neofox_cli(): def _read_data(input_file, patients_data, mhc_database: MhcDatabase) -> Tuple[List[Neoantigen], List[Patient]]: # parse patient data - logger.info("Parsing patients data from: {}".format(patients_data)) - patients = ModelConverter.parse_patients_file(patients_data, mhc_database) - logger.info("Loaded {} patients".format(len(patients))) + if os.path.isfile(patients_data): + logger.info("Parsing patients data from: {}".format(patients_data)) + patients = ModelConverter.parse_patients_file(patients_data, mhc_database) + logger.info("Loaded {} patients".format(len(patients))) + else: + logger.error(f"Patient data file {patients_data} does not exist. Is the spelling correct?") + raise FileNotFoundError("Patient data file does not exist.") # parse the neoantigen candidate data + if not os.path.isfile(input_file): # first check if the file exists + logger.error(f"Neoantigen candidate file {input_file} does not exist. Is the spelling correct?") + raise FileNotFoundError("Neoantigen candidate file does not exist.") + if input_file.endswith('.txt') or input_file.endswith('.tsv'): logger.info("Parsing candidate neoantigens from: {}".format(input_file)) neoantigens = ModelConverter.parse_candidate_file(input_file) @@ -356,7 +365,8 @@ def neofox_epitope_cli(): patients=patients, log_file_name=log_file_name, num_cpus=num_cpus, - reference_folder=reference_folder + reference_folder=reference_folder, + verbose = args.verbose ).get_annotations() _write_results_epitopes( @@ -376,12 +386,19 @@ def _read_data_epitopes( # parse patient data patients = [] - if patients_data is not None: + if os.path.isfile(patients_data): logger.info("Parsing patients data from: {}".format(patients_data)) patients = ModelConverter.parse_patients_file(patients_data, mhc_database) logger.info("Loaded {} patients".format(len(patients))) + else: + logger.error(f"Patient data file {patients_data} does not exist. Is the spelling correct?") + raise FileNotFoundError("Patient data file does not exist.") # parse the neoantigen candidate data + if not os.path.isfile(input_file): # first check if the file exists + logger.error(f"Neoepitope candidate file {input_file} does not exist. Is the spelling correct?") + raise FileNotFoundError("Neoantigen candidate file does not exist.") + if input_file.endswith('.txt') or input_file.endswith('.tsv'): logger.info("Parsing candidate neoepitopes from: {}".format(input_file)) neoepitopes = ModelConverter.parse_candidate_neoepitopes_file(input_file, mhc_database, organism) diff --git a/neofox/helpers/runner.py b/neofox/helpers/runner.py index d705288c..1ab6a49e 100755 --- a/neofox/helpers/runner.py +++ b/neofox/helpers/runner.py @@ -31,7 +31,7 @@ def __init__(self, verbose=True): def run_command(self, cmd, print_log=True, **kwargs): if print_log and self.verbose: - logger.info("Starting command: {}".format(" ".join(cmd))) + logger.debug("Starting command: {}".format(" ".join(cmd))) start = time.time() process = subprocess.Popen( self._preprocess_command(cmd), @@ -43,10 +43,10 @@ def run_command(self, cmd, print_log=True, **kwargs): return_code = process.returncode end = time.time() if print_log and self.verbose: - logger.info("Elapsed time {} seconds".format(round(end - start, 3))) + logger.debug("Elapsed time {} seconds".format(round(end - start, 3))) if return_code == 0: if print_log and self.verbose: - logger.info("Finished command correctly!") + logger.debug("Finished command correctly!") else: logger.error("Finished command with return code {}".format(return_code)) logger.error(self._decode(output)) diff --git a/neofox/model/validation.py b/neofox/model/validation.py index 2b1737bf..cdc48df0 100755 --- a/neofox/model/validation.py +++ b/neofox/model/validation.py @@ -45,6 +45,11 @@ FIELD_WILD_TYPE_XMER = "[WT]_+-13_AA_(SNV)_/_-15_AA_to_STOP_(INDEL)" FIELD_MUTATED_XMER = "+-13_AA_(SNV)_/_-15_AA_to_STOP_(INDEL)" +MIN_MHCI_EPITOPE_LENGTH = 8 +MAX_MHCI_EPITOPE_LENGTH = 14 + +MIN_MHCII_EPITOPE_LENGTH = 9 +MAX_MHCII_EPITOPE_LENGTH = 20000 GENES_BY_MOLECULE = { Mhc2Name.DR: [Mhc2GeneName.DRB1], @@ -127,31 +132,31 @@ def validate_neoepitope(neoepitope: PredictedEpitope, organism: str): length_mutated_peptide = len(neoepitope.mutated_peptide) if has_mhc_i: ModelValidator.validate_mhc_allele_representation(neoepitope.allele_mhc_i, organism=organism) - assert ModelValidator.is_mhci_peptide_length_valid(length_mutated_peptide), \ - "Mutated MHC-I peptide has a non supported length of {}".format(length_mutated_peptide) + assert ModelValidator.is_peptide_length_valid(length_mutated_peptide, MIN_MHCI_EPITOPE_LENGTH, MAX_MHCI_EPITOPE_LENGTH), \ + f"Mutated MHC-I peptide has a non supported length of {length_mutated_peptide}. MHC-I peptides with lengths between {MIN_MHCI_EPITOPE_LENGTH} and {MAX_MHCI_EPITOPE_LENGTH} are supported." elif has_mhc_ii: ModelValidator.validate_mhc2_isoform_representation(neoepitope.isoform_mhc_i_i, organism=organism) - assert ModelValidator.is_mhcii_peptide_length_valid(length_mutated_peptide), \ - "Mutated MHC-II peptide has a non supported length of {}".format(length_mutated_peptide) + assert ModelValidator.is_peptide_length_valid(length_mutated_peptide, MIN_MHCII_EPITOPE_LENGTH, MAX_MHCII_EPITOPE_LENGTH), \ + f"Mutated MHC-II peptide has a non supported length of {length_mutated_peptide}. MHC-II peptides with lengths between {MIN_MHCII_EPITOPE_LENGTH} and {MAX_MHCII_EPITOPE_LENGTH} are supported" else: - assert ModelValidator.is_mhci_peptide_length_valid(length_mutated_peptide) or \ - ModelValidator.is_mhcii_peptide_length_valid(length_mutated_peptide), \ - "Mutated peptide has a non supported length of {}".format(length_mutated_peptide) + assert ModelValidator.is_peptide_length_valid(length_mutated_peptide, MIN_MHCI_EPITOPE_LENGTH, MAX_MHCI_EPITOPE_LENGTH) or \ + ModelValidator.is_peptide_length_valid(length_mutated_peptide, MIN_MHCII_EPITOPE_LENGTH, MAX_MHCII_EPITOPE_LENGTH), \ + f"Mutated peptide has a non supported length of {length_mutated_peptide}. MHC-I peptides with lengths between {MIN_MHCI_EPITOPE_LENGTH} and {MAX_MHCI_EPITOPE_LENGTH} and MHC-II peptides with lengths between {MIN_MHCII_EPITOPE_LENGTH} and {MAX_MHCII_EPITOPE_LENGTH} are supported." if has_wt_peptide: length_wt_peptide = len(neoepitope.wild_type_peptide) assert length_wt_peptide == length_mutated_peptide, \ "Neoepitope does not have the same length of wildtype and mutated sequence. Both sequences should have the same length in case of point mutations. wildTypePeptide shall be empty, specially in the case of neoantigen candidates derived from other sources than SNVs." if has_mhc_i: - assert ModelValidator.is_mhci_peptide_length_valid(length_wt_peptide), \ - "Mutated MHC-I peptide has a non supported length of {}".format(length_wt_peptide) + assert ModelValidator.is_peptide_length_valid(length_wt_peptide, MIN_MHCI_EPITOPE_LENGTH, MAX_MHCI_EPITOPE_LENGTH), \ + f"Wildtype MHC-I peptide has a non supported length of {length_wt_peptide}. MHC-I peptides with lengths between {MIN_MHCI_EPITOPE_LENGTH} and {MAX_MHCI_EPITOPE_LENGTH} are supported." elif has_mhc_ii: - assert ModelValidator.is_mhcii_peptide_length_valid(length_wt_peptide), \ - "Mutated MHC-II peptide has a non supported length of {}".format(length_wt_peptide) + assert ModelValidator.is_peptide_length_valid(length_wt_peptide, MIN_MHCII_EPITOPE_LENGTH, MAX_MHCII_EPITOPE_LENGTH), \ + f"Wildtype MHC-II peptide has a non supported length of {length_wt_peptide}. MHC-II peptides with lengths between {MIN_MHCII_EPITOPE_LENGTH} and {MAX_MHCII_EPITOPE_LENGTH} are supported." else: - assert ModelValidator.is_mhci_peptide_length_valid(length_wt_peptide) or \ - ModelValidator.is_mhcii_peptide_length_valid(length_wt_peptide), \ - "Mutated peptide has a non supported length of {}".format(length_wt_peptide) + assert ModelValidator.is_peptide_length_valid(length_wt_peptide, MIN_MHCI_EPITOPE_LENGTH, MAX_MHCI_EPITOPE_LENGTH) or \ + ModelValidator.is_peptide_length_valid(length_wt_peptide, MIN_MHCII_EPITOPE_LENGTH, MAX_MHCII_EPITOPE_LENGTH), \ + f"Wildtype peptide has a non supported length of {length_wt_peptide}. MHC-I peptides with lengths between {MIN_MHCI_EPITOPE_LENGTH} and {MAX_MHCI_EPITOPE_LENGTH} and MHC-II peptides with lengths between {MIN_MHCII_EPITOPE_LENGTH} and {MAX_MHCII_EPITOPE_LENGTH} are supported." # check the expression values ModelValidator._validate_expression_values(neoepitope) @@ -160,12 +165,13 @@ def validate_neoepitope(neoepitope: PredictedEpitope, organism: str): raise NeofoxDataValidationException(e) @staticmethod - def is_mhcii_peptide_length_valid(length_mutated_peptide): - return 9 <= length_mutated_peptide <= 20000 + def is_peptide_length_valid(length_peptide, min_len, max_len): + return min_len <= length_peptide <= max_len + # NOTE: this method is needed as it is called in neofox_epitope.py @staticmethod - def is_mhci_peptide_length_valid(length_mutated_peptide): - return 8 <= length_mutated_peptide <= 14 + def is_mhci_peptide_length_valid(length_peptide): + return MIN_MHCI_EPITOPE_LENGTH <= length_peptide <= MAX_MHCI_EPITOPE_LENGTH @staticmethod def is_mhcii_epitope(neoepitope): diff --git a/neofox/neofox.py b/neofox/neofox.py index 644a715f..ff996284 100755 --- a/neofox/neofox.py +++ b/neofox/neofox.py @@ -50,13 +50,14 @@ def __init__( log_file_name=None, reference_folder: ReferenceFolder = None, configuration: DependenciesConfiguration = None, - verbose=True, + verbose = False, configuration_file=None, rank_mhci_threshold=neofox.RANK_MHCI_THRESHOLD_DEFAULT, rank_mhcii_threshold=neofox.RANK_MHCII_THRESHOLD_DEFAULT, with_all_neoepitopes=False): - initialise_logs(logfile=log_file_name, verbose=verbose) + self.verbose = verbose + initialise_logs(logfile=log_file_name, verbose=self.verbose) logger.info("Loading reference data...") self.rank_mhci_threshold = rank_mhci_threshold @@ -71,7 +72,7 @@ def __init__( # NOTE: uses the reference folder and config passed as a parameter if exists, this is here to make it # testable with fake objects self.reference_folder = ( - reference_folder if reference_folder else ReferenceFolder(verbose=verbose) + reference_folder if reference_folder else ReferenceFolder(verbose=self.verbose) ) # NOTE: makes this call to force the loading of the available alleles here self.reference_folder.get_available_alleles() @@ -215,7 +216,8 @@ def send_to_client(self, dask_client): self.log_file_name, self.rank_mhci_threshold, self.rank_mhcii_threshold, - self.with_all_neoepitopes + self.with_all_neoepitopes, + self.verbose ) ) annotated_neoantigens = dask_client.gather(futures) @@ -238,11 +240,12 @@ def annotate_neoantigen( log_file_name: str, rank_mhci_threshold = neofox.RANK_MHCI_THRESHOLD_DEFAULT, rank_mhcii_threshold=neofox.RANK_MHCII_THRESHOLD_DEFAULT, - with_all_neoepitopes=False + with_all_neoepitopes=False, + verbose = False ): # the logs need to be initialised inside every dask job - initialise_logs(log_file_name) - logger.info("Starting neoantigen annotation with peptide={}".format(neoantigen.mutated_xmer)) + initialise_logs(log_file_name, verbose) + logger.debug("Starting neoantigen annotation with peptide={}".format(neoantigen.mutated_xmer)) start = time.time() try: annotated_neoantigen = NeoantigenAnnotator( @@ -258,7 +261,7 @@ def annotate_neoantigen( logger.error("Error processing patient {}".format(patient.to_dict())) raise e end = time.time() - logger.info( + logger.debug( "Elapsed time for annotating neoantigen for peptide={}: {} seconds".format( neoantigen.mutated_xmer, int(end - start)) ) @@ -268,8 +271,7 @@ def annotate_neoantigen( def initialise_logs(logfile, verbose=False): if logfile is not None: logzero.logfile(logfile) - # TODO: this does not work if verbose: - logzero.loglevel(logging.INFO) + logzero.loglevel(logging.DEBUG) else: - logzero.loglevel(logging.WARN) + logzero.loglevel(logging.INFO) diff --git a/neofox/neofox_epitope.py b/neofox/neofox_epitope.py index 37d7e79a..a0dd077c 100755 --- a/neofox/neofox_epitope.py +++ b/neofox/neofox_epitope.py @@ -46,9 +46,10 @@ def __init__( log_file_name=None, reference_folder: ReferenceFolder = None, configuration: DependenciesConfiguration = None, - verbose=True, + verbose=False, configuration_file=None): + self.verbose = verbose initialise_logs(logfile=log_file_name, verbose=verbose) logger.info("Loading reference data...") @@ -153,6 +154,7 @@ def send_to_client(self, dask_client): future_tcell_predictor, future_self_similarity, self.log_file_name, + self.verbose, ) ) annotated_neoantigens = dask_client.gather(futures) @@ -179,10 +181,11 @@ def annotate_neoepitope( tcell_predictor: TcellPrediction, self_similarity: SelfSimilarityCalculator, log_file_name: str, + verbose = False ): # the logs need to be initialised inside every dask job - initialise_logs(log_file_name) - logger.info("Starting neoepitope annotation with peptide={}".format(neoepitope.mutated_peptide)) + initialise_logs(log_file_name, verbose=verbose) + logger.debug("Starting neoepitope annotation with peptide={}".format(neoepitope.mutated_peptide)) start = time.time() try: annotated_neoantigen = NeoepitopeAnnotator( @@ -195,7 +198,7 @@ def annotate_neoepitope( logger.error("Error processing neoantigen {}".format(neoepitope.to_dict())) raise e end = time.time() - logger.info( + logger.debug( "Elapsed time for annotating neoantigen for peptide={}: {} seconds".format( neoepitope.mutated_peptide, int(end - start)) ) @@ -221,8 +224,7 @@ def _conditional_expression_imputation(self) -> List[PredictedEpitope]: def initialise_logs(logfile, verbose=False): if logfile is not None: logzero.logfile(logfile) - # TODO: this does not work if verbose: - logzero.loglevel(logging.INFO) + logzero.loglevel(logging.DEBUG) else: - logzero.loglevel(logging.WARN) + logzero.loglevel(logging.INFO) diff --git a/neofox/published_features/neoantigen_fitness/neoantigen_fitness.py b/neofox/published_features/neoantigen_fitness/neoantigen_fitness.py index 8d604c45..566b7997 100755 --- a/neofox/published_features/neoantigen_fitness/neoantigen_fitness.py +++ b/neofox/published_features/neoantigen_fitness/neoantigen_fitness.py @@ -33,7 +33,7 @@ def __init__(self, iedb_blastp_runner: BlastpRunner): def get_pathogen_similarity(self, peptide: str): pathsim = self.iedb_blastp_runner.calculate_similarity_database(peptide=peptide) - logger.info( + logger.debug( "Peptide {} has a pathogen similarity of {}".format(peptide, pathsim) ) return pathsim From ed6a06adddc5e5bbb8c17c4a35469100b719a2ee Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Tue, 9 Jan 2024 18:03:43 +0100 Subject: [PATCH 18/66] Added initial version of VaxRank tests --- .../integration_test_tools.py | 58 +++++++++++++++- .../tests/integration_tests/test_vaxrank.py | 66 +++++++++++++++++++ 2 files changed, 123 insertions(+), 1 deletion(-) create mode 100644 neofox/tests/integration_tests/test_vaxrank.py diff --git a/neofox/tests/integration_tests/integration_test_tools.py b/neofox/tests/integration_tests/integration_test_tools.py index 53c60695..b301c57d 100755 --- a/neofox/tests/integration_tests/integration_test_tools.py +++ b/neofox/tests/integration_tests/integration_test_tools.py @@ -24,7 +24,7 @@ from Bio.Alphabet.IUPAC import IUPACData from neofox.helpers.epitope_helper import EpitopeHelper -from neofox.model.factories import MhcFactory +from neofox.model.factories import MhcFactory, NeoepitopeFactory from neofox.model.neoantigen import PredictedEpitope, MhcAllele, Mhc2Isoform from neofox.references.references import ReferenceFolder, DependenciesConfiguration, ORGANISM_HOMO_SAPIENS, \ ORGANISM_MUS_MUSCULUS @@ -107,6 +107,62 @@ def get_h2_two_test_b(h2_database): ], h2_database ) +def get_test_epitopes(hla_database): + # Epitopes of the neoantigen DSVVIFSGEGSDEFTQGYIYFHKAPSP + # for testing VaxRank + affinity_values = [1.74, 7.67, 802.78, 2057.1, 770.35, 5002.44] + + neoepitopes = [ + NeoepitopeFactory.build_neoepitope( + mutated_peptide = "VIFSGEGSDEF", + wild_type_peptide = "VIFSGEGSDEL", + allele_mhc_i = "HLA-A*24:02", + mhc_database = hla_database, + organism = "human" + ), + NeoepitopeFactory.build_neoepitope( + mutated_peptide = "VIFSGEGSDEF", + wild_type_peptide = "VIFSGEGSDEL", + allele_mhc_i = "HLA-A*02:01", + mhc_database = hla_database, + organism = "human" + ), + NeoepitopeFactory.build_neoepitope( + mutated_peptide = "VIFSGEGSDEF", + wild_type_peptide = "VIFSGEGSDEL", + allele_mhc_i = "HLA-B*15:01", + mhc_database = hla_database, + organism = "human" + ), + NeoepitopeFactory.build_neoepitope( + mutated_peptide = "IFSGEGSDEFT", + wild_type_peptide = "IFSGEGSDELT", + allele_mhc_i = "HLA-A*24:02", + mhc_database = hla_database, + organism = "human" + ), + NeoepitopeFactory.build_neoepitope( + mutated_peptide = "IFSGEGSDEFT", + wild_type_peptide = "IFSGEGSDELT", + allele_mhc_i = "HLA-A*02:01", + mhc_database = hla_database, + organism = "human" + ), + # this epitope will not be considered for vaxrank calculation + NeoepitopeFactory.build_neoepitope( + mutated_peptide = "IFSGEGSDEFT", + wild_type_peptide = "IFSGEGSDELT", + allele_mhc_i = "HLA-B*15:01", + mhc_database = hla_database, + organism = "human" + ), + ] + + for i, a in enumerate(affinity_values): + neoepitopes[i].affinity_mutated = affinity_values[i] + + return neoepitopes + mutations_with_rare_aminoacids = [ ("UTTDSDGKF", "UTTDSWGKF"), # this is an epitope from IEDB of length 9 ("XTTDSDGKF", "XTTDSWGKF"), diff --git a/neofox/tests/integration_tests/test_vaxrank.py b/neofox/tests/integration_tests/test_vaxrank.py new file mode 100644 index 00000000..b2f4d1c6 --- /dev/null +++ b/neofox/tests/integration_tests/test_vaxrank.py @@ -0,0 +1,66 @@ +# +# Copyright (c) 2020-2030 Translational Oncology at the Medical Center of the Johannes Gutenberg-University Mainz gGmbH. +# +# This file is part of Neofox +# (see https://github.com/tron-bioinformatics/neofox). +# +# This program is free software: you can redistribute it and/or modify +# it under the terms of the GNU General Public License as published by +# the Free Software Foundation, either version 3 of the License, or +# (at your option) any later version. +# +# This program is distributed in the hope that it will be useful, +# but WITHOUT ANY WARRANTY; without even the implied warranty of +# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the +# GNU General Public License for more details. +# +# You should have received a copy of the GNU General Public License +# along with this program. If not, see .# +import os +from unittest import TestCase + +import neofox.tests.integration_tests.integration_test_tools as integration_test_tools + +from neofox.helpers.runner import Runner +from neofox.model.neoantigen import PredictedEpitope +from neofox.published_features.vaxrank.vaxrank import ( + VaxRank, +) + + +class TestVaxRank(TestCase): + def setUp(self): + self.references, self.configuration = integration_test_tools.load_references() + self.runner = Runner() + self.vaxrank = VaxRank() + self.hla_database = self.references.get_mhc_database() + + def test_vaxrank(self): + """ + Test whether the VaxRank calculation works correct. + + The binding score of one list of neoepitopes is calculated and compared + with the true value. As floating point calculations can differ between + architectures and the vaxrank annotations are rounded, we only compare on + almost equal. + Additionaly it is checked if the total score and the total imputed score + is correctly calculated. + """ + EXPRESSION_SCORE = 10.4 + IMPUTED_SCORE = 7.6 + + neoepitopes = integration_test_tools.get_test_epitopes(self.hla_database) + + annot = VaxRank().get_annotations( + epitope_predictions = neoepitopes, + expression_score = EXPRESSION_SCORE, + imputed_score = IMPUTED_SCORE + ) + + # to access the values of the Vaxrank annotation even if the order changes + annot_dict = {a.name: float(a.value) for a in annot} + + # the binding score calculated by hand + assert abs(annot_dict["Vaxrank_bindingScore"] - 2.108220805) < 0.001 + assert abs(annot_dict["Vaxrank_bindingScore"] * EXPRESSION_SCORE - annot_dict["Vaxrank_totalScore"]) < 0.001 + assert abs(annot_dict["Vaxrank_bindingScore"] * IMPUTED_SCORE - annot_dict["Vaxrank_totalScore_imputed"]) < 0.001 From 3fd26f5a6718b48d9c7e7b41e4d95f6ee7a300db Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Thu, 11 Jan 2024 09:24:09 +0100 Subject: [PATCH 19/66] Update to NetMHCIIpan 4.3 * Adapted parsed columns to new output * Considered that ERROR is now spelled Error in NetMHCIIpan 4.3 * Version increase --- neofox/MHC_predictors/netmhcpan/netmhcIIpan_prediction.py | 7 +++---- neofox/__init__.py | 2 +- 2 files changed, 4 insertions(+), 5 deletions(-) diff --git a/neofox/MHC_predictors/netmhcpan/netmhcIIpan_prediction.py b/neofox/MHC_predictors/netmhcpan/netmhcIIpan_prediction.py index 8e7c111e..4c0b715c 100755 --- a/neofox/MHC_predictors/netmhcpan/netmhcIIpan_prediction.py +++ b/neofox/MHC_predictors/netmhcpan/netmhcIIpan_prediction.py @@ -124,18 +124,17 @@ def _parse_netmhcpan_output(self, lines: str) -> List[PredictedEpitope]: for line in lines.splitlines(): line = line.rstrip().lstrip() if line: - if line.startswith(("#", "-", "Number", "Temporary", "Seq", "ERROR", "Pos")): + if line.startswith(("#", "-", "Number", "Temporary", "Seq", "ERROR", "Pos", "Error")): continue line = line.split() - line = line[0:-1] if len(line) > 12 else line pred_epitope = PredictedEpitope( position=int(line[0]), isoform_mhc_i_i=self.mhc_parser.parse_mhc2_isoform(line[1]), core=str(line[4]), mutated_peptide=line[2], - affinity_mutated=float(line[11]), - rank_mutated=float(line[8]), + affinity_mutated=float(line[13]), + rank_mutated=float(line[9]), ) pred_epitope.neofox_annotations.annotations.extend( self.get_additional_netmhcpan_annotations(line) diff --git a/neofox/__init__.py b/neofox/__init__.py index 3bf714be..06420eba 100755 --- a/neofox/__init__.py +++ b/neofox/__init__.py @@ -18,7 +18,7 @@ # along with this program. If not, see .# -VERSION = "1.1.1.dev4" +VERSION = "1.1.1.dev5" REFERENCE_FOLDER_ENV = "NEOFOX_REFERENCE_FOLDER" NEOFOX_BLASTP_ENV = "NEOFOX_BLASTP" From 085431621783ee9a91c8bc9611efb111f3a052e3 Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Fri, 12 Jan 2024 11:55:57 +0100 Subject: [PATCH 20/66] Cleaned up vaxrank integration tests --- neofox/__init__.py | 2 +- neofox/tests/fake_classes.py | 6 ++ .../integration_test_tools.py | 55 --------------- .../tests/integration_tests/test_vaxrank.py | 69 +++++++++++++++---- 4 files changed, 61 insertions(+), 71 deletions(-) diff --git a/neofox/__init__.py b/neofox/__init__.py index 3bf714be..06420eba 100755 --- a/neofox/__init__.py +++ b/neofox/__init__.py @@ -18,7 +18,7 @@ # along with this program. If not, see .# -VERSION = "1.1.1.dev4" +VERSION = "1.1.1.dev5" REFERENCE_FOLDER_ENV = "NEOFOX_REFERENCE_FOLDER" NEOFOX_BLASTP_ENV = "NEOFOX_BLASTP" diff --git a/neofox/tests/fake_classes.py b/neofox/tests/fake_classes.py index 3f26d331..82e050e4 100755 --- a/neofox/tests/fake_classes.py +++ b/neofox/tests/fake_classes.py @@ -93,3 +93,9 @@ class FakeH2Database(H2Database): def __init__(self): super().__init__(database_filename=pkg_resources.resource_filename( neofox.references.__name__, "h2_database_allele_list.csv")) + +class FakePredictedEpitope(PredictedEpitope): + + def __init__(self, mutated_epitope, allele, affinity): + self.mutated_peptide = mutated_epitope + self.affinity_mutated = affinity diff --git a/neofox/tests/integration_tests/integration_test_tools.py b/neofox/tests/integration_tests/integration_test_tools.py index b301c57d..0eabb91b 100755 --- a/neofox/tests/integration_tests/integration_test_tools.py +++ b/neofox/tests/integration_tests/integration_test_tools.py @@ -107,61 +107,6 @@ def get_h2_two_test_b(h2_database): ], h2_database ) -def get_test_epitopes(hla_database): - # Epitopes of the neoantigen DSVVIFSGEGSDEFTQGYIYFHKAPSP - # for testing VaxRank - affinity_values = [1.74, 7.67, 802.78, 2057.1, 770.35, 5002.44] - - neoepitopes = [ - NeoepitopeFactory.build_neoepitope( - mutated_peptide = "VIFSGEGSDEF", - wild_type_peptide = "VIFSGEGSDEL", - allele_mhc_i = "HLA-A*24:02", - mhc_database = hla_database, - organism = "human" - ), - NeoepitopeFactory.build_neoepitope( - mutated_peptide = "VIFSGEGSDEF", - wild_type_peptide = "VIFSGEGSDEL", - allele_mhc_i = "HLA-A*02:01", - mhc_database = hla_database, - organism = "human" - ), - NeoepitopeFactory.build_neoepitope( - mutated_peptide = "VIFSGEGSDEF", - wild_type_peptide = "VIFSGEGSDEL", - allele_mhc_i = "HLA-B*15:01", - mhc_database = hla_database, - organism = "human" - ), - NeoepitopeFactory.build_neoepitope( - mutated_peptide = "IFSGEGSDEFT", - wild_type_peptide = "IFSGEGSDELT", - allele_mhc_i = "HLA-A*24:02", - mhc_database = hla_database, - organism = "human" - ), - NeoepitopeFactory.build_neoepitope( - mutated_peptide = "IFSGEGSDEFT", - wild_type_peptide = "IFSGEGSDELT", - allele_mhc_i = "HLA-A*02:01", - mhc_database = hla_database, - organism = "human" - ), - # this epitope will not be considered for vaxrank calculation - NeoepitopeFactory.build_neoepitope( - mutated_peptide = "IFSGEGSDEFT", - wild_type_peptide = "IFSGEGSDELT", - allele_mhc_i = "HLA-B*15:01", - mhc_database = hla_database, - organism = "human" - ), - ] - - for i, a in enumerate(affinity_values): - neoepitopes[i].affinity_mutated = affinity_values[i] - - return neoepitopes mutations_with_rare_aminoacids = [ ("UTTDSDGKF", "UTTDSWGKF"), # this is an epitope from IEDB of length 9 diff --git a/neofox/tests/integration_tests/test_vaxrank.py b/neofox/tests/integration_tests/test_vaxrank.py index b2f4d1c6..1f5710df 100644 --- a/neofox/tests/integration_tests/test_vaxrank.py +++ b/neofox/tests/integration_tests/test_vaxrank.py @@ -20,47 +20,86 @@ from unittest import TestCase import neofox.tests.integration_tests.integration_test_tools as integration_test_tools +from neofox.model.factories import AnnotationFactory from neofox.helpers.runner import Runner from neofox.model.neoantigen import PredictedEpitope from neofox.published_features.vaxrank.vaxrank import ( VaxRank, ) +from neofox.tests.fake_classes import FakePredictedEpitope +# TODO: maybe create Fake Class Predicted Epitope to easier create test epitopes class TestVaxRank(TestCase): def setUp(self): self.references, self.configuration = integration_test_tools.load_references() self.runner = Runner() self.vaxrank = VaxRank() + self.AnnotationFactory = AnnotationFactory() self.hla_database = self.references.get_mhc_database() + # Generate epitopes for test case + # NOTE: these should remain the same, to check if something in the + # vaxrank calculation has changed + epitopes = [ + "VIFSGEGSDEF", "VIFSGEGSDEF", "VIFSGEGSDEF", "IFSGEGSDEFT", "IFSGEGSDEFT", "IFSGEGSDEFT" + ] + alleles = [ + "HLA-A*24:02", "HLA-A*02:01", "HLA-B*15:01", "HLA-A*24:02", "HLA-A*02:01", "HLA-B*15:01" + ] + affinities = [1.74, 7.67, 802.78, 2057.1, 770.35, 5002.44] + self.pred_epitopes = [FakePredictedEpitope(e, m, a) for e, m, a in zip(epitopes, alleles, affinities)] + + # expected binding value + self.TRUE_BINDING_SCORE = 2.1082247484257532 + + def test_vaxrank_binding(self): + """ + Test if the VaxRank binding score is calculated correctly. + + The binding score of one list of neoepitopes is calculated and compared + with the manually calculated value. As floating point calculations can + differ between architectures and the vaxrank annotations are rounded, we + only compare on almost equal. + """ + + binding_score = self.vaxrank.total_binding(self.pred_epitopes) + + self.assertAlmostEqual(binding_score, self.TRUE_BINDING_SCORE) + def test_vaxrank(self): """ Test whether the VaxRank calculation works correct. - The binding score of one list of neoepitopes is calculated and compared - with the true value. As floating point calculations can differ between - architectures and the vaxrank annotations are rounded, we only compare on - almost equal. - Additionaly it is checked if the total score and the total imputed score - is correctly calculated. + It is checked if the total score and the total imputed score is correctly calculated. """ EXPRESSION_SCORE = 10.4 IMPUTED_SCORE = 7.6 - neoepitopes = integration_test_tools.get_test_epitopes(self.hla_database) + binding_score = self.vaxrank.total_binding(self.pred_epitopes) + + # expected annotation + expected_total_score = AnnotationFactory.build_annotation( + value=EXPRESSION_SCORE * binding_score, + name="Vaxrank_totalScore" + ) + expected_total_score_imputed = AnnotationFactory.build_annotation( + value=IMPUTED_SCORE * binding_score, + name="Vaxrank_totalScore_imputed" + ) - annot = VaxRank().get_annotations( - epitope_predictions = neoepitopes, + # calculated annotation + annot = self.vaxrank.get_annotations( + epitope_predictions = self.pred_epitopes, expression_score = EXPRESSION_SCORE, imputed_score = IMPUTED_SCORE ) - # to access the values of the Vaxrank annotation even if the order changes - annot_dict = {a.name: float(a.value) for a in annot} + # access the values of the Vaxrank annotation even if the order changes + annot_dict = {a.name: a.value for a in annot} - # the binding score calculated by hand - assert abs(annot_dict["Vaxrank_bindingScore"] - 2.108220805) < 0.001 - assert abs(annot_dict["Vaxrank_bindingScore"] * EXPRESSION_SCORE - annot_dict["Vaxrank_totalScore"]) < 0.001 - assert abs(annot_dict["Vaxrank_bindingScore"] * IMPUTED_SCORE - annot_dict["Vaxrank_totalScore_imputed"]) < 0.001 + # since strings with the rounded value are returned by the get_annotations + # function, we can compare here on equality + assert annot_dict["Vaxrank_totalScore"] == expected_total_score.value + assert annot_dict["Vaxrank_totalScore_imputed"] == expected_total_score_imputed.value From ea53cb4eadd52106e5f0dba5a29e28de72af7c00 Mon Sep 17 00:00:00 2001 From: "Kress, Luis" Date: Fri, 12 Jan 2024 12:02:21 +0100 Subject: [PATCH 21/66] Revert changes in integration_test_tools.py --- neofox/tests/integration_tests/integration_test_tools.py | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/neofox/tests/integration_tests/integration_test_tools.py b/neofox/tests/integration_tests/integration_test_tools.py index 0eabb91b..53c60695 100755 --- a/neofox/tests/integration_tests/integration_test_tools.py +++ b/neofox/tests/integration_tests/integration_test_tools.py @@ -24,7 +24,7 @@ from Bio.Alphabet.IUPAC import IUPACData from neofox.helpers.epitope_helper import EpitopeHelper -from neofox.model.factories import MhcFactory, NeoepitopeFactory +from neofox.model.factories import MhcFactory from neofox.model.neoantigen import PredictedEpitope, MhcAllele, Mhc2Isoform from neofox.references.references import ReferenceFolder, DependenciesConfiguration, ORGANISM_HOMO_SAPIENS, \ ORGANISM_MUS_MUSCULUS @@ -107,7 +107,6 @@ def get_h2_two_test_b(h2_database): ], h2_database ) - mutations_with_rare_aminoacids = [ ("UTTDSDGKF", "UTTDSWGKF"), # this is an epitope from IEDB of length 9 ("XTTDSDGKF", "XTTDSWGKF"), From 199d21e38f72960ec93e61e71af9f78c8df40609 Mon Sep 17 00:00:00 2001 From: "Kress, Luis" Date: Fri, 12 Jan 2024 12:04:54 +0100 Subject: [PATCH 22/66] Clean up comments in test_vaxrank.py --- neofox/tests/integration_tests/test_vaxrank.py | 6 ++---- 1 file changed, 2 insertions(+), 4 deletions(-) diff --git a/neofox/tests/integration_tests/test_vaxrank.py b/neofox/tests/integration_tests/test_vaxrank.py index 1f5710df..32a64485 100644 --- a/neofox/tests/integration_tests/test_vaxrank.py +++ b/neofox/tests/integration_tests/test_vaxrank.py @@ -59,9 +59,7 @@ def test_vaxrank_binding(self): Test if the VaxRank binding score is calculated correctly. The binding score of one list of neoepitopes is calculated and compared - with the manually calculated value. As floating point calculations can - differ between architectures and the vaxrank annotations are rounded, we - only compare on almost equal. + with the manually calculated value. """ binding_score = self.vaxrank.total_binding(self.pred_epitopes) @@ -72,7 +70,7 @@ def test_vaxrank(self): """ Test whether the VaxRank calculation works correct. - It is checked if the total score and the total imputed score is correctly calculated. + Check if the total score and the total imputed score are calculated correctly. """ EXPRESSION_SCORE = 10.4 IMPUTED_SCORE = 7.6 From 3f810e27b970b7bef0d620309edcf44df8e9c487 Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Mon, 15 Jan 2024 11:33:40 +0100 Subject: [PATCH 23/66] Moved input file validation into validation.py script --- neofox/command_line.py | 67 ++++++++++++++------------------- neofox/model/validation.py | 77 ++++++++++++++++++++++++++++++++++++++ 2 files changed, 106 insertions(+), 38 deletions(-) diff --git a/neofox/command_line.py b/neofox/command_line.py index 51222a6f..5f2c4ed6 100755 --- a/neofox/command_line.py +++ b/neofox/command_line.py @@ -24,7 +24,7 @@ import neofox import neofox.neofox from neofox.model.neoantigen import Neoantigen, Patient, PredictedEpitope -from neofox.model.validation import ModelValidator +from neofox.model.validation import ModelValidator, InputValidator from neofox.neofox import NeoFox import os from neofox.model.conversion import ModelConverter @@ -208,30 +208,23 @@ def neofox_cli(): def _read_data(input_file, patients_data, mhc_database: MhcDatabase) -> Tuple[List[Neoantigen], List[Patient]]: - # parse patient data - if os.path.isfile(patients_data): - logger.info("Parsing patients data from: {}".format(patients_data)) - patients = ModelConverter.parse_patients_file(patients_data, mhc_database) - logger.info("Loaded {} patients".format(len(patients))) - else: - logger.error(f"Patient data file {patients_data} does not exist. Is the spelling correct?") - raise FileNotFoundError("Patient data file does not exist.") + InputValidator.validate_patient_file(patients_data) - # parse the neoantigen candidate data - if not os.path.isfile(input_file): # first check if the file exists - logger.error(f"Neoantigen candidate file {input_file} does not exist. Is the spelling correct?") - raise FileNotFoundError("Neoantigen candidate file does not exist.") + logger.info("Parsing patients data from: {}".format(patients_data)) + patients = ModelConverter.parse_patients_file(patients_data, mhc_database) + logger.info("Loaded {} patients".format(len(patients))) - if input_file.endswith('.txt') or input_file.endswith('.tsv'): - logger.info("Parsing candidate neoantigens from: {}".format(input_file)) - neoantigens = ModelConverter.parse_candidate_file(input_file) - logger.info("Loaded {} candidate neoantigens".format(len(neoantigens))) - elif input_file.endswith('.json') : + + if input_file.endswith('.json') : logger.info("Parsing candidate neoantigens from: {}".format(input_file)) neoantigens = ModelConverter.parse_neoantigens_json_file(input_file) logger.info("Loaded {} candidate neoantigens".format(len(neoantigens))) else: - raise ValueError('Not supported input file extension: {}'.format(input_file)) + InputValidator.validate_input_file(input_file) + + logger.info("Parsing candidate neoantigens from: {}".format(input_file)) + neoantigens = ModelConverter.parse_candidate_file(input_file) + logger.info("Loaded {} candidate neoantigens".format(len(neoantigens))) neoantigens_patient_ids = set(neoantigen.patient_identifier for neoantigen in neoantigens) patient_ids = set(patient.identifier for patient in patients) @@ -385,31 +378,29 @@ def _read_data_epitopes( input_file, patients_data, mhc_database: MhcDatabase, organism: str) -> Tuple[List[PredictedEpitope], List[Patient]]: # parse patient data - patients = [] - if os.path.isfile(patients_data): - logger.info("Parsing patients data from: {}".format(patients_data)) - patients = ModelConverter.parse_patients_file(patients_data, mhc_database) - logger.info("Loaded {} patients".format(len(patients))) - else: - logger.error(f"Patient data file {patients_data} does not exist. Is the spelling correct?") - raise FileNotFoundError("Patient data file does not exist.") + InputValidator.validate_patient_file(patients_data) + + logger.info("Parsing patients data from: {}".format(patients_data)) + patients = ModelConverter.parse_patients_file(patients_data, mhc_database) + logger.info("Loaded {} patients".format(len(patients))) + # parse the neoantigen candidate data - if not os.path.isfile(input_file): # first check if the file exists - logger.error(f"Neoepitope candidate file {input_file} does not exist. Is the spelling correct?") - raise FileNotFoundError("Neoantigen candidate file does not exist.") + if input_file.endswith('.json'): + # TODO: add support for input in JSON format + # logger.info("Parsing candidate neoepitopes from: {}".format(input_file)) + # neoepitopes = ModelConverter.parse_neoepitopes_json_file(input_file) + # logger.info("Loaded {} candidate neoepitopes".format(len(neoepitopes))) + raise ValueError('Not supported input file extension: {}'.format(input_file)) + + else: + InputValidator.validate_input_file(input_file, epitope_mode = True) - if input_file.endswith('.txt') or input_file.endswith('.tsv'): logger.info("Parsing candidate neoepitopes from: {}".format(input_file)) neoepitopes = ModelConverter.parse_candidate_neoepitopes_file(input_file, mhc_database, organism) logger.info("Loaded {} candidate neoepitopes".format(len(neoepitopes))) - # TODO: add support for input in JSON format - #elif input_file.endswith('.json') : - # logger.info("Parsing candidate neoepitopes from: {}".format(input_file)) - # neoepitopes = ModelConverter.parse_neoepitopes_json_file(input_file) - # logger.info("Loaded {} candidate neoepitopes".format(len(neoepitopes))) - else: - raise ValueError('Not supported input file extension: {}'.format(input_file)) + + return neoepitopes, patients diff --git a/neofox/model/validation.py b/neofox/model/validation.py index cdc48df0..23b71509 100755 --- a/neofox/model/validation.py +++ b/neofox/model/validation.py @@ -17,6 +17,9 @@ # You should have received a copy of the GNU General Public License # along with this program. If not, see .# import betterproto +import csv +import os +import pandas as pd from Bio.Alphabet.IUPAC import ExtendedIUPACProtein from Bio.Data import IUPACData from neofox.exceptions import NeofoxDataValidationException @@ -383,3 +386,77 @@ def validate_mhc2_gene(allele: MhcAllele): assert allele.gene in Mhc2GeneName.__members__, \ "MHC II allele is not valid {} at {}".format( allele.gene, allele.full_name) if allele.full_name != "" else "Gene from MHC II allele is empty" + + +class InputValidator(object): + """ + Ensure that the input files are in the correct format. + """ + + input_antigen = [ + "gene", "wildTypeXmer", "mutatedXmer", "patientIdentifier", "rnaExpression", + "rnaVariantAlleleFrequency", "dnaVariantAlleleFrequency" + ] + + input_epitope = [ + "gene", "wildTypePeptide", "mutatedPeptide", "patientIdentifier", "rnaExpression", + "alleleMhcI", "isoformMhcII", "rnaVariantAlleleFrequency", "dnaVariantAlleleFrequency" + ] + + columns_patient_file = ["identifier", "mhcIAlleles", "mhcIIAlleles"] + + @staticmethod + def validate_input_file(file_path: str, epitope_mode: bool = True): + required_columns = InputValidator.input_epitope if epitope_mode else InputValidator.input_antigen + + try: + assert InputValidator._file_exists(file_path), \ + f"File {file_path} does not exist. Is the spelling correct?" + assert InputValidator._is_tab_separated(file_path), \ + f"File {file_path} is not tab separated." + assert InputValidator._required_columns_given(file_path, required_columns), \ + f"File {file_path} does not contain all required columns. Required columns are: {required_columns}" + + except AssertionError as e: + logger.error("Input file validation failed.") + raise NeofoxDataValidationException(e) + + @staticmethod + def validate_patient_file(file_path: str): + try: + assert InputValidator._file_exists(file_path), \ + f"File {file_path} does not exist. Is the spelling correct?" + assert InputValidator._is_tab_separated(file_path), \ + f"File {file_path} is not tab separated." + assert InputValidator._required_columns_given(file_path, InputValidator.columns_patient_file), \ + f"File {file_path} does not contain all required columns. Required columns are: {InputValidator.columns_patient_file}" + + except AssertionError as e: + logger.error("Input file validation failed.") + raise NeofoxDataValidationException(e) + + @staticmethod + def _file_exists(file_path: str): + if file_path is None: + return False + return os.path.isfile(file_path) + + @staticmethod + def _is_tab_separated(file_path: str): + try: + # infers the delimiter from the first 100 rows + reader = pd.read_csv(file_path, sep = None, nrows = 100, iterator = True) + delimiter = reader._engine.data.dialect.delimiter + + logger.debug(f"File {file_path} is '{delimiter}' separated.".encode("unicode_escape").decode("utf-8")) + assert delimiter == "\t" + return True + + except Exception as e: + logger.error(f"File {file_path} is not tab separated.") + raise e + + @staticmethod + def _required_columns_given(file_path: str, required_columns: list): + header = pd.read_csv(file_path, sep = "\t", nrows = 1) + return all(rq in header.columns for rq in required_columns) From 39725063bc0713aa6c74dd7d1cbaf0f6bab38f8d Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Mon, 15 Jan 2024 14:38:39 +0100 Subject: [PATCH 24/66] Set epitope mode in validation to correct default value --- neofox/model/validation.py | 18 +++++++++--------- 1 file changed, 9 insertions(+), 9 deletions(-) diff --git a/neofox/model/validation.py b/neofox/model/validation.py index 23b71509..4767ad69 100755 --- a/neofox/model/validation.py +++ b/neofox/model/validation.py @@ -406,7 +406,7 @@ class InputValidator(object): columns_patient_file = ["identifier", "mhcIAlleles", "mhcIIAlleles"] @staticmethod - def validate_input_file(file_path: str, epitope_mode: bool = True): + def validate_input_file(file_path: str, epitope_mode: bool = False): required_columns = InputValidator.input_epitope if epitope_mode else InputValidator.input_antigen try: @@ -426,8 +426,7 @@ def validate_patient_file(file_path: str): try: assert InputValidator._file_exists(file_path), \ f"File {file_path} does not exist. Is the spelling correct?" - assert InputValidator._is_tab_separated(file_path), \ - f"File {file_path} is not tab separated." + assert InputValidator._is_tab_separated(file_path) assert InputValidator._required_columns_given(file_path, InputValidator.columns_patient_file), \ f"File {file_path} does not contain all required columns. Required columns are: {InputValidator.columns_patient_file}" @@ -445,17 +444,18 @@ def _file_exists(file_path: str): def _is_tab_separated(file_path: str): try: # infers the delimiter from the first 100 rows - reader = pd.read_csv(file_path, sep = None, nrows = 100, iterator = True) - delimiter = reader._engine.data.dialect.delimiter - - logger.debug(f"File {file_path} is '{delimiter}' separated.".encode("unicode_escape").decode("utf-8")) - assert delimiter == "\t" - return True + reader = pd.read_csv(file_path, sep = None, nrows = 100, iterator = True, engine = 'python') except Exception as e: logger.error(f"File {file_path} is not tab separated.") raise e + delimiter = reader._engine.data.dialect.delimiter + logger.debug(f"File {file_path} is '{delimiter}' separated.".encode("unicode_escape").decode("utf-8")) + assert delimiter == "\t", \ + f"File {file_path} is not tab separated." + return True + @staticmethod def _required_columns_given(file_path: str, required_columns: list): header = pd.read_csv(file_path, sep = "\t", nrows = 1) From a2c92d559ec43ab51a811e5ba938ddd91cf07448 Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Mon, 15 Jan 2024 15:02:47 +0100 Subject: [PATCH 25/66] Clean up unnecessary comment --- neofox/tests/integration_tests/test_vaxrank.py | 2 -- 1 file changed, 2 deletions(-) diff --git a/neofox/tests/integration_tests/test_vaxrank.py b/neofox/tests/integration_tests/test_vaxrank.py index 32a64485..ccf46492 100644 --- a/neofox/tests/integration_tests/test_vaxrank.py +++ b/neofox/tests/integration_tests/test_vaxrank.py @@ -29,8 +29,6 @@ ) from neofox.tests.fake_classes import FakePredictedEpitope -# TODO: maybe create Fake Class Predicted Epitope to easier create test epitopes - class TestVaxRank(TestCase): def setUp(self): self.references, self.configuration = integration_test_tools.load_references() From 8419243944952beafe1d3ce765d4242ed08cd6ac Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Tue, 16 Jan 2024 17:22:48 +0100 Subject: [PATCH 26/66] Adapt neofox version for refesources_versions.json in installer.py --- neofox/references/installer.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/neofox/references/installer.py b/neofox/references/installer.py index 972e4714..7ae2ebbc 100644 --- a/neofox/references/installer.py +++ b/neofox/references/installer.py @@ -92,7 +92,7 @@ def _save_resources_versions( resources_version = [ Resource(name="netMHCpan", version="4.1"), - Resource(name="netMHCIIpan", version="4.0"), + Resource(name="netMHCIIpan", version="4.3"), Resource(name="mixMHCpred", version="2.2"), Resource(name="mixMHC2pred", version="2.0.2"), iedb_resource, From 360d1fb875669f8b5e6621c6f6b769bfd071e386 Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Mon, 22 Jan 2024 11:59:08 +0100 Subject: [PATCH 27/66] Move validation of input files to model conversion --- neofox/command_line.py | 9 --------- neofox/model/conversion.py | 10 ++++++++++ neofox/model/validation.py | 12 ++++++------ 3 files changed, 16 insertions(+), 15 deletions(-) diff --git a/neofox/command_line.py b/neofox/command_line.py index 5f2c4ed6..0f2620b2 100755 --- a/neofox/command_line.py +++ b/neofox/command_line.py @@ -208,8 +208,6 @@ def neofox_cli(): def _read_data(input_file, patients_data, mhc_database: MhcDatabase) -> Tuple[List[Neoantigen], List[Patient]]: - InputValidator.validate_patient_file(patients_data) - logger.info("Parsing patients data from: {}".format(patients_data)) patients = ModelConverter.parse_patients_file(patients_data, mhc_database) logger.info("Loaded {} patients".format(len(patients))) @@ -220,8 +218,6 @@ def _read_data(input_file, patients_data, mhc_database: MhcDatabase) -> Tuple[Li neoantigens = ModelConverter.parse_neoantigens_json_file(input_file) logger.info("Loaded {} candidate neoantigens".format(len(neoantigens))) else: - InputValidator.validate_input_file(input_file) - logger.info("Parsing candidate neoantigens from: {}".format(input_file)) neoantigens = ModelConverter.parse_candidate_file(input_file) logger.info("Loaded {} candidate neoantigens".format(len(neoantigens))) @@ -378,8 +374,6 @@ def _read_data_epitopes( input_file, patients_data, mhc_database: MhcDatabase, organism: str) -> Tuple[List[PredictedEpitope], List[Patient]]: # parse patient data - InputValidator.validate_patient_file(patients_data) - logger.info("Parsing patients data from: {}".format(patients_data)) patients = ModelConverter.parse_patients_file(patients_data, mhc_database) logger.info("Loaded {} patients".format(len(patients))) @@ -392,10 +386,7 @@ def _read_data_epitopes( # neoepitopes = ModelConverter.parse_neoepitopes_json_file(input_file) # logger.info("Loaded {} candidate neoepitopes".format(len(neoepitopes))) raise ValueError('Not supported input file extension: {}'.format(input_file)) - else: - InputValidator.validate_input_file(input_file, epitope_mode = True) - logger.info("Parsing candidate neoepitopes from: {}".format(input_file)) neoepitopes = ModelConverter.parse_candidate_neoepitopes_file(input_file, mhc_database, organism) logger.info("Loaded {} candidate neoepitopes".format(len(neoepitopes))) diff --git a/neofox/model/conversion.py b/neofox/model/conversion.py index 4f6399b3..94445a42 100755 --- a/neofox/model/conversion.py +++ b/neofox/model/conversion.py @@ -19,12 +19,14 @@ from typing import List import pandas as pd import betterproto +import os from betterproto import Casing from neofox import NOT_AVAILABLE_VALUE, MHC_II, MHC_I from collections import defaultdict import orjson as json import numpy as np from neofox.model.mhc_parser import MhcParser +from neofox.model.validation import InputValidator from neofox.model.neoantigen import ( Neoantigen, Patient, @@ -48,6 +50,8 @@ def parse_candidate_file(candidate_file: str) -> List[Neoantigen]: :return neoantigens in model objects """ + InputValidator.validate_input_file(candidate_file) + data = pd.read_csv( candidate_file, sep="\t", # NOTE: forces the types of every column to avoid pandas setting the wrong type for corner cases @@ -73,6 +77,9 @@ def parse_candidate_file(candidate_file: str) -> List[Neoantigen]: @staticmethod def parse_candidate_neoepitopes_file(candidate_file: str, mhc_database: MhcDatabase, organism: str) -> List[PredictedEpitope]: + + InputValidator.validate_input_file(candidate_file, epitope_mode = True) + data = pd.read_csv( candidate_file, sep="\t", # NOTE: forces the types of every column to avoid pandas setting the wrong type for corner cases @@ -104,6 +111,9 @@ def parse_patients_file(patients_file: str, mhc_database: MhcDatabase) -> List[P :return: the parsed CSV into model objects """ split_comma_separated_list = lambda x: x.split(",") + + InputValidator.validate_patient_file(patients_file) + df = pd.read_csv( patients_file, sep="\t", diff --git a/neofox/model/validation.py b/neofox/model/validation.py index 4767ad69..79c60d2c 100755 --- a/neofox/model/validation.py +++ b/neofox/model/validation.py @@ -390,20 +390,20 @@ def validate_mhc2_gene(allele: MhcAllele): class InputValidator(object): """ - Ensure that the input files are in the correct format. + Ensure that the input files are in correct format. """ input_antigen = [ - "gene", "wildTypeXmer", "mutatedXmer", "patientIdentifier", "rnaExpression", - "rnaVariantAlleleFrequency", "dnaVariantAlleleFrequency" + "wildTypeXmer", "mutatedXmer", "patientIdentifier", ] input_epitope = [ - "gene", "wildTypePeptide", "mutatedPeptide", "patientIdentifier", "rnaExpression", - "alleleMhcI", "isoformMhcII", "rnaVariantAlleleFrequency", "dnaVariantAlleleFrequency" + "wildTypePeptide", "mutatedPeptide" ] - columns_patient_file = ["identifier", "mhcIAlleles", "mhcIIAlleles"] + columns_patient_file = [ + "identifier" + ] @staticmethod def validate_input_file(file_path: str, epitope_mode: bool = False): From 5a97d3a5cbe06bee1c1f96cabce3f5fea149f491 Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Tue, 23 Jan 2024 15:27:25 +0100 Subject: [PATCH 28/66] Update netMHCIIpan version in README and documentation. --- README.md | 8 ++++---- docs/source/01_overview.md | 4 ++-- docs/source/02_installation.md | 19 ++++++++----------- 3 files changed, 14 insertions(+), 17 deletions(-) diff --git a/README.md b/README.md index 7181c980..5ead3f0d 100755 --- a/README.md +++ b/README.md @@ -28,7 +28,7 @@ NeoFox covers the following neoantigen features and prediction algorithms: | Name | Reference | DOI | |---------------------------------------------------------|--------------------------------------------------------------------------|-------------------------------------------------------------------------------------------| | MHC I binding affinity/rank score (netMHCpan-v4.1) | Reynisson et al, 2020, Nucleic Acids Research | https://doi.org/10.4049/jimmunol.1700893 | -| MHC II binding affinity/rank score (netMHCIIpan-v4.0) | Reynisson et al, 2020, Nucleic Acids Research | https://doi.org/10.1111/imm.12889 | +| MHC II binding affinity/rank score (netMHCIIpan-v4.3) | Nilsson et al, 2023, Science Adv. | https://doi.org/10.1126/sciadv.adj6367 | | MixMHCpred score v2.2 § | Bassani-Sternberg et al., 2017, PLoS Comp Bio; Gfeller, 2018, J Immunol. | https://doi.org/10.1371/journal.pcbi.1005725 , https://doi.org/10.4049/jimmunol.1800914 | | MixMHC2pred score v2.0.2 § | Racle et al, 2019, Nat. Biotech. 2019 | https://doi.org/10.1038/s41587-019-0289-6 | | Differential Agretopicity Index (DAI) | Duan et al, 2014, JEM; Ghorani et al., 2018, Ann Oncol. | https://doi.org/10.1084/jem.20141308 | @@ -54,7 +54,7 @@ NeoFox depends on the following tools: - Python >=3.7, <=3.8 - BLAST 2.10.1 - netMHCpan 4.1 -- netMHCIIpan 4.0 +- netMHCIIpan 4.3 - MixMHCpred 2.2 (optional) - MixMHC2pred 2.0.2 (optional) - PRIME 2.0 (optional) @@ -97,7 +97,7 @@ The optional config file with the paths to the dependencies can look like this: NEOFOX_REFERENCE_FOLDER=path/to/reference/folder NEOFOX_BLASTP=path/to/ncbi-blast-2.10.1+/bin/blastp NEOFOX_NETMHCPAN=path/to/netMHCpan-4.1/netMHCpan -NEOFOX_NETMHC2PAN=path/to/netMHCIIpan-4.0/netMHCIIpan +NEOFOX_NETMHC2PAN=path/to/netMHCIIpan-4.3/netMHCIIpan NEOFOX_MIXMHCPRED=path/to/MixMHCpred-2.2/MixMHCpred NEOFOX_MIXMHC2PRED=path/to/MixMHC2pred-2.0.1/MixMHC2pred_unix NEOFOX_MAKEBLASTDB=path/to/ncbi-blast-2.8.1+/bin/makeblastdb @@ -161,4 +161,4 @@ where: ## 5 Output data -The output data is returned by default in a short wide tab separated values file (`--with-table`). Optionally, it can be provided in JSON format (`--with-json`). \ No newline at end of file +The output data is returned by default in a short wide tab separated values file (`--with-table`). Optionally, it can be provided in JSON format (`--with-json`). diff --git a/docs/source/01_overview.md b/docs/source/01_overview.md index a3401c2a..d7f2c449 100644 --- a/docs/source/01_overview.md +++ b/docs/source/01_overview.md @@ -29,7 +29,7 @@ A list of implemented features and their references are given in Table 1. Please | Name | Reference | DOI | |---------------------------------------------------------|--------------------------------------------------------------------------|-------------------------------------------------------------------------------------------| | MHC I binding affinity/rank score (netMHCpan-v4.1) | Reynisson et al., 2020, Nucleic Acids Res. | https://doi.org/10.1093/nar/gkaa379 | -| MHC II binding affinity/rank score (netMHCIIpan-v4.0) | Reynisson et al., 2020, Nucleic Acids Res. | https://doi.org/10.1093/nar/gkaa379 | +| MHC II binding affinity/rank score (netMHCIIpan-v4.3) | Nilsson et al., 2023, Science Adv. | https://doi.org/10.1126/sciadv.adj6367 | | MixMHCpred score v2.2 § | Bassani-Sternberg et al., 2017, PLoS Comp Bio; Gfeller, 2018, J Immunol. | https://doi.org/10.1371/journal.pcbi.1005725 , https://doi.org/10.4049/jimmunol.1800914 | | MixMHC2pred score v2.0.2 § | Racle et al., 2019, Nat. Biotech. 2019 | https://doi.org/10.1038/s41587-019-0289-6 | | Differential Agretopicity Index (DAI) | Duan et al., 2014, JEM; Ghorani et al., 2018, Ann Oncol. | https://doi.org/10.1084/jem.20141308 | @@ -67,4 +67,4 @@ Happy annotation and modelling! For questions, please contact Franziska Lang ([franziska.lang@tron-mainz.de](mailto:franziska.lang@tron-mainz.de)) or Pablo Riesgo Ferreiro ([pablo.riesgoferreiro@tron-mainz.de](mailto:pablo.riesgoferreiro@tron-mainz.de)). ## How to cite -Franziska Lang, Pablo Riesgo-Ferreiro, Martin Löwer, Ugur Sahin, Barbara Schrörs, **NeoFox: annotating neoantigen candidates with neoantigen features**, Bioinformatics, Volume 37, Issue 22, 15 November 2021, Pages 4246–4247, [https://doi.org/10.1093/bioinformatics/btab344](https://doi.org/10.1093/bioinformatics/btab344) \ No newline at end of file +Franziska Lang, Pablo Riesgo-Ferreiro, Martin Löwer, Ugur Sahin, Barbara Schrörs, **NeoFox: annotating neoantigen candidates with neoantigen features**, Bioinformatics, Volume 37, Issue 22, 15 November 2021, Pages 4246–4247, [https://doi.org/10.1093/bioinformatics/btab344](https://doi.org/10.1093/bioinformatics/btab344) diff --git a/docs/source/02_installation.md b/docs/source/02_installation.md index a3e7b57a..7dc9ebf8 100755 --- a/docs/source/02_installation.md +++ b/docs/source/02_installation.md @@ -18,7 +18,7 @@ distribute the software or provide a direct URL to download it. Please make sure the sites indicated below. - NetMHCpan-4.1: https://services.healthtech.dtu.dk/service.php?NetMHCpan-4.1 (`netMHCpan-4.1b.Linux.tar.gz`) -- NetMHCIIpan-4.0: https://services.healthtech.dtu.dk/software.php (`netMHCIIpan-4.0.Linux.tar.gz`) +- NetMHCIIpan-4.3: https://services.healthtech.dtu.dk/software.php (`netMHCIIpan-4.3.Linux.tar.gz`) Please also check the licences of the other third party dependencies ( e.g. listed in the docker recipe `Dockerfile`). @@ -93,26 +93,23 @@ export NEOFOX_NETMHCPAN=/path/to/netMHCpan-4.1/netMHCpan Configure NetMHCpan as explained in the file `netMHCpan-4.1/netMHCpan-4.1.readme` -#### Install NetMHCIIpan-4.0 +#### Install NetMHCIIpan-4.3 -NetMHCIIpan-4.0 can be downloaded by academic users from https://services.healthtech.dtu.dk/software.php +NetMHCIIpan-4.3 can be downloaded by academic users from https://services.healthtech.dtu.dk/software.php ``` -tar -xvf netMHCIIpan-4.0.Linux.tar.gz -cd netMHCIIpan-4.0 -# download the data -wget http://www.cbs.dtu.dk/services/NetMHCIIpan-4.0/data.Linux.tar.gz -tar -xvf data.Linux.tar.gz +tar -xvf netMHCIIpan-4.3.Linux.tar.gz +cd netMHCIIpan-4.3 # install tcsh shell interpreter if not available yet sudo apt-get install tcsh ``` Optionally set the environment variable pointing to `netMHCIIpan`, otherwise neofox will look for it in the path. ``` -export NEOFOX_NETMHC2PAN=/path/to/netMHCIIpan-4.0/netMHCIIpan +export NEOFOX_NETMHC2PAN=/path/to/netMHCIIpan-4.3/netMHCIIpan ``` -Configure NetMHCIIpan-4.0 as explained in the file `netMHCIIpan-4.0/netMHCIIpan-4.0.readme` +Configure NetMHCIIpan-4.3 as explained in the file `netMHCIIpan-4.3/netMHCIIpan-4.3.readme` #### Install MixMHCpred-2.2 (recommended but optional) @@ -214,4 +211,4 @@ neofox --input-file /path/to/test_data.txt --patient-data /path/to/test_patients The resulting output files can be compared to the following test output files: * [test_neoantigen_candidates_annotated.tsv](_static/test_neoantigen_candidates_annotated.tsv) -* [test_neoantigen_candidates_annotated.json](_static/test_neoantigen_candidates_annotated.json) \ No newline at end of file +* [test_neoantigen_candidates_annotated.json](_static/test_neoantigen_candidates_annotated.json) From 55667f9f575ad8df50915b5b739f46631c8e3805 Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Tue, 23 Jan 2024 15:50:24 +0100 Subject: [PATCH 29/66] Remove logging of iedb score --- neofox/published_features/iedb_immunogenicity/iedb.py | 1 - 1 file changed, 1 deletion(-) diff --git a/neofox/published_features/iedb_immunogenicity/iedb.py b/neofox/published_features/iedb_immunogenicity/iedb.py index aaebabb3..68b6f26d 100755 --- a/neofox/published_features/iedb_immunogenicity/iedb.py +++ b/neofox/published_features/iedb_immunogenicity/iedb.py @@ -140,7 +140,6 @@ def calculate_iedb_immunogenicity( try: if peptide != "-": score = self.predict_immunogenicity(peptide, mhc_allele.name if mhc_allele else None) - logger.info(score) except (ValueError, AttributeError): pass return score From 52f9c701c136053cfd659cea17f9f69f48816799 Mon Sep 17 00:00:00 2001 From: "Kress, Luis" Date: Fri, 26 Jan 2024 08:54:51 +0100 Subject: [PATCH 30/66] Version increase --- neofox/__init__.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/neofox/__init__.py b/neofox/__init__.py index 06420eba..e0169e5f 100755 --- a/neofox/__init__.py +++ b/neofox/__init__.py @@ -18,7 +18,7 @@ # along with this program. If not, see .# -VERSION = "1.1.1.dev5" +VERSION = "1.1.1.dev6" REFERENCE_FOLDER_ENV = "NEOFOX_REFERENCE_FOLDER" NEOFOX_BLASTP_ENV = "NEOFOX_BLASTP" From c40cbc23835afbcd72ea14e3089c5398a29c2ade Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Mon, 29 Jan 2024 16:22:45 +0100 Subject: [PATCH 31/66] Consider also MHC allele for resolving ties when selectiong the best epitope * increase version * update docs on selection of best predicted epitope --- docs/source/03_02_output_data.md | 2 ++ neofox/__init__.py | 2 +- neofox/helpers/epitope_helper.py | 12 +++++++----- 3 files changed, 10 insertions(+), 6 deletions(-) diff --git a/docs/source/03_02_output_data.md b/docs/source/03_02_output_data.md index 202b6007..3860a140 100755 --- a/docs/source/03_02_output_data.md +++ b/docs/source/03_02_output_data.md @@ -130,6 +130,8 @@ In addition, all logging output is appended to a log file with the suffix "*/.log*", where the folder is set by `--output-folder` and the prefix can be set with `--output-prefix`. +> Note on best predicted epitope selection: If no unique selection of the best predicted epitope can be made due to ties, the lexicographically first epitope is returned. If there are nevertheless two or more alleles that have equally good predicted values for the peptide, the lexicographically first one is reported. These cases can be analysed using the `--with-all-epitopes` options. + ### Tabular format An output table with the suffix "*_neoantigen_candidates_annotated.tsv*" is created. diff --git a/neofox/__init__.py b/neofox/__init__.py index e0169e5f..262956b2 100755 --- a/neofox/__init__.py +++ b/neofox/__init__.py @@ -18,7 +18,7 @@ # along with this program. If not, see .# -VERSION = "1.1.1.dev6" +VERSION = "1.1.1.dev7" REFERENCE_FOLDER_ENV = "NEOFOX_REFERENCE_FOLDER" NEOFOX_BLASTP_ENV = "NEOFOX_BLASTP" diff --git a/neofox/helpers/epitope_helper.py b/neofox/helpers/epitope_helper.py index 0ce2fea9..251c3946 100755 --- a/neofox/helpers/epitope_helper.py +++ b/neofox/helpers/epitope_helper.py @@ -154,24 +154,26 @@ def get_empty_epitope(): def select_best_by_rank(predictions: List[PredictedEpitope]) -> PredictedEpitope: """ Returns the peptide with the lowest (ie: meaning highest) rank and in case of tie first peptide on - alphabetical order to ensure determinism + alphabetical order to ensure determinism. If there are two or more alleles with the best score, the + first in alphabetical order is returned to ensure determinism. """ - return max(predictions, key=lambda p: (-p.rank_mutated, p.mutated_peptide)) \ + return max(predictions, key=lambda p: (-p.rank_mutated, p.mutated_peptide, p.allele_mhc_i, p.isoform_mhc_i_i.name)) \ if predictions is not None and len(predictions) > 0 else EpitopeHelper.get_empty_epitope() @staticmethod def select_best_by_affinity(predictions: List[PredictedEpitope], maximum=False) -> PredictedEpitope: """ Returns the peptide with the highest affinity score and in case of tie first peptide on - alphabetical order to ensure determinism + alphabetical order to ensure determinism. If there are two or more alleles with the best score, the + first in alphabetical order is returned to ensure determinism. By default the highest affinity score is the lowest (ie: netmhc family) if maximum=True then the highest score is the highest (ie: mixmhcpred and PRIME) """ if maximum: - return max(predictions, key=lambda p: (p.affinity_mutated, p.mutated_peptide)) \ + return max(predictions, key=lambda p: (p.affinity_mutated, p.mutated_peptide, p.allele_mhc_i.name, p.isoform_mhc_i_i)) \ if predictions is not None and len(predictions) > 0 else EpitopeHelper.get_empty_epitope() else: - return max(predictions, key=lambda p: (-p.affinity_mutated, p.mutated_peptide)) \ + return max(predictions, key=lambda p: (-p.affinity_mutated, p.mutated_peptide, p.allele_mhc_i.name, p.isoform_mhc_i_i)) \ if predictions is not None and len(predictions) > 0 else EpitopeHelper.get_empty_epitope() @staticmethod From b3e5be67189e258d5daeb66b1764026a33b0d81d Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Tue, 30 Jan 2024 11:16:47 +0100 Subject: [PATCH 32/66] Improve readability of select_best_by_affinity --- neofox/helpers/epitope_helper.py | 22 ++++++++++++++++++---- 1 file changed, 18 insertions(+), 4 deletions(-) diff --git a/neofox/helpers/epitope_helper.py b/neofox/helpers/epitope_helper.py index 251c3946..b4ef9642 100755 --- a/neofox/helpers/epitope_helper.py +++ b/neofox/helpers/epitope_helper.py @@ -170,11 +170,25 @@ def select_best_by_affinity(predictions: List[PredictedEpitope], maximum=False) score is the highest (ie: mixmhcpred and PRIME) """ if maximum: - return max(predictions, key=lambda p: (p.affinity_mutated, p.mutated_peptide, p.allele_mhc_i.name, p.isoform_mhc_i_i)) \ - if predictions is not None and len(predictions) > 0 else EpitopeHelper.get_empty_epitope() + return max( + predictions, + key=lambda p: ( + p.affinity_mutated, p.mutated_peptide, + p.allele_mhc_i.name, p.isoform_mhc_i_i.name + ) + ) \ + if predictions is not None and len(predictions) > 0 \ + else EpitopeHelper.get_empty_epitope() else: - return max(predictions, key=lambda p: (-p.affinity_mutated, p.mutated_peptide, p.allele_mhc_i.name, p.isoform_mhc_i_i)) \ - if predictions is not None and len(predictions) > 0 else EpitopeHelper.get_empty_epitope() + return max( + predictions, + key=lambda p: ( + -p.affinity_mutated, p.mutated_peptide, + p.allele_mhc_i.name, p.isoform_mhc_i_i.name + ) + ) \ + if predictions is not None and len(predictions) > 0 \ + else EpitopeHelper.get_empty_epitope() @staticmethod def remove_peptides_in_proteome(predictions: List[PredictedEpitope], uniprot From a0dad51ea7bf52759a2d7bc82b551e6a2c046272 Mon Sep 17 00:00:00 2001 From: "Kress, Luis" Date: Wed, 7 Feb 2024 14:44:17 +0100 Subject: [PATCH 33/66] Add comment on epitope selection step and column naming to documentation --- docs/source/03_02_output_data.md | 2 ++ 1 file changed, 2 insertions(+) diff --git a/docs/source/03_02_output_data.md b/docs/source/03_02_output_data.md index 3860a140..dcf6547d 100755 --- a/docs/source/03_02_output_data.md +++ b/docs/source/03_02_output_data.md @@ -8,6 +8,8 @@ The user gets the neoantigen annotations in all formats. Despite different structures, both formats provide the same content with the exception of the metadata on the annotations which is only present in the JSON format. +> For each neoantigen candidate (`mutatedXmer`), the best subsequences (epitopes) based on MHC prediction are annotated. Columns that refer to a predicted epitope consist of 3 components, each separated by "_". The first part contains the tool name, the second part contains the selection method of the best epitope and the third part contains the name of the value. Example: "ToolName_SelectionMethod_ValueName" -> NetMHCpan_bestRank_peptide (literally: the peptide with the best predicted NetMHCpan rank). + The following table describes each of the annotations in the output: **TABLE 1** From a6d967b893060304db566210eb9032d2bdef9835 Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Thu, 21 Mar 2024 14:59:42 +0100 Subject: [PATCH 34/66] Remove orjson: * Replaced orjson by json * Updated tests to run with NetMHCpan4.3 * Removed prime_mixmhcpred argument which was only used for logging --- neofox/MHC_predictors/MixMHCpred/mixmhcpred.py | 2 +- neofox/MHC_predictors/prime.py | 2 +- neofox/command_line.py | 6 +++--- neofox/helpers/blastp_runner.py | 2 +- neofox/helpers/epitope_helper.py | 2 +- neofox/helpers/mhc_helper.py | 4 ++-- neofox/model/conversion.py | 2 +- .../integration_tests/test_alternative_mutation.py | 10 +++++----- .../integration_tests/test_best_multiple_binder.py | 10 +++++----- neofox/tests/integration_tests/test_neofox.py | 6 +++--- requirements.txt | 1 - 11 files changed, 23 insertions(+), 24 deletions(-) diff --git a/neofox/MHC_predictors/MixMHCpred/mixmhcpred.py b/neofox/MHC_predictors/MixMHCpred/mixmhcpred.py index 7ca163b8..a4c85fcd 100755 --- a/neofox/MHC_predictors/MixMHCpred/mixmhcpred.py +++ b/neofox/MHC_predictors/MixMHCpred/mixmhcpred.py @@ -80,7 +80,7 @@ def run(self, neoantigen: Neoantigen, mhc: List[Mhc1], uniprot): ) if len(potential_ligand_sequences) > 0: mhc1_alleles = self.parsed_mhc_alleles.get_mixmhc_allele_representation(self.configuration.mix_mhc_pred_alleles_list, - [a for m in mhc for a in m.alleles], 'MixMHCpred') + [a for m in mhc for a in m.alleles]) if len(mhc1_alleles) > 0: self.results = self._mixmhcprediction(mhc1_alleles, potential_ligand_sequences) else: diff --git a/neofox/MHC_predictors/prime.py b/neofox/MHC_predictors/prime.py index 6c64c39f..9bc26327 100755 --- a/neofox/MHC_predictors/prime.py +++ b/neofox/MHC_predictors/prime.py @@ -89,7 +89,7 @@ def run(self, neoantigen: Neoantigen, mhc: List[Mhc1], uniprot): ) if len(potential_ligand_sequences) > 0: mhc1_alleles = self.parsed_mhc_alleles.get_mixmhc_allele_representation(self.configuration.prime_alleles_list, - [a for m in mhc for a in m.alleles], 'PRIME') + [a for m in mhc for a in m.alleles]) if len(mhc1_alleles) > 0: self.results = self._prime(mhc1_alleles, potential_ligand_sequences) else: diff --git a/neofox/command_line.py b/neofox/command_line.py index 0f2620b2..68c11c38 100755 --- a/neofox/command_line.py +++ b/neofox/command_line.py @@ -20,7 +20,7 @@ from typing import Tuple, List, Dict import dotenv from logzero import logger -import orjson as json +import json import neofox import neofox.neofox from neofox.model.neoantigen import Neoantigen, Patient, PredictedEpitope @@ -263,7 +263,7 @@ def _write_results(neoantigens, output_folder, output_prefix, with_all_neoepitop ) output_features = os.path.join(output_folder, "{}_neoantigen_candidates_annotated.json".format(output_prefix)) - with open(output_features, "wb") as f: + with open(output_features, "w") as f: f.write(json.dumps(ModelConverter.objects2json(neoantigens))) @@ -424,5 +424,5 @@ def _write_results_epitopes(neoepitopes: List[PredictedEpitope], output_folder, if neoepitopes: output_features = os.path.join(output_folder, "{}_neoepitope_candidates_annotated.json".format(output_prefix)) - with open(output_features, "wb") as f: + with open(output_features, "w") as f: f.write(json.dumps(ModelConverter.objects2json(neoepitopes))) diff --git a/neofox/helpers/blastp_runner.py b/neofox/helpers/blastp_runner.py index d015f424..58807832 100755 --- a/neofox/helpers/blastp_runner.py +++ b/neofox/helpers/blastp_runner.py @@ -17,7 +17,7 @@ # You should have received a copy of the GNU General Public License # along with this program. If not, see .# from math import exp, log -import orjson as json +import json import subprocess from Bio import pairwise2 from Bio.SubsMat import MatrixInfo as matlist diff --git a/neofox/helpers/epitope_helper.py b/neofox/helpers/epitope_helper.py index b4ef9642..33540faf 100755 --- a/neofox/helpers/epitope_helper.py +++ b/neofox/helpers/epitope_helper.py @@ -157,7 +157,7 @@ def select_best_by_rank(predictions: List[PredictedEpitope]) -> PredictedEpitope alphabetical order to ensure determinism. If there are two or more alleles with the best score, the first in alphabetical order is returned to ensure determinism. """ - return max(predictions, key=lambda p: (-p.rank_mutated, p.mutated_peptide, p.allele_mhc_i, p.isoform_mhc_i_i.name)) \ + return max(predictions, key=lambda p: (-p.rank_mutated, p.mutated_peptide, p.allele_mhc_i.name, p.isoform_mhc_i_i.name)) \ if predictions is not None and len(predictions) > 0 else EpitopeHelper.get_empty_epitope() @staticmethod diff --git a/neofox/helpers/mhc_helper.py b/neofox/helpers/mhc_helper.py index 20cfe677..a35ade93 100644 --- a/neofox/helpers/mhc_helper.py +++ b/neofox/helpers/mhc_helper.py @@ -40,7 +40,7 @@ def __init__(self, mhc_parser: MhcParser): self.mhc_parser = mhc_parser @staticmethod - def get_mixmhc_allele_representation(file_alleles, mhc_alleles: List[MhcAllele], prime_mixmhcpred): + def get_mixmhc_allele_representation(file_alleles, mhc_alleles: List[MhcAllele]): """ loads file with available HLA II alleles for MixMHCpred and PRIME prediction, returns set :return: @@ -57,7 +57,7 @@ def get_mixmhc_allele_representation(file_alleles, mhc_alleles: List[MhcAllele], not_available_alleles = list(set(converted_mhc_alleles).difference(available_alleles)) if len(not_available_alleles) > 0: logger.warning( - "MHC I alleles {} are not supported by {}.".format(",".join(not_available_alleles), prime_mixmhcpred) + "MHC I alleles {} are not supported by MixMHCpred.".format(",".join(not_available_alleles)) ) return list( diff --git a/neofox/model/conversion.py b/neofox/model/conversion.py index 94445a42..ebe8edec 100755 --- a/neofox/model/conversion.py +++ b/neofox/model/conversion.py @@ -23,7 +23,7 @@ from betterproto import Casing from neofox import NOT_AVAILABLE_VALUE, MHC_II, MHC_I from collections import defaultdict -import orjson as json +import json import numpy as np from neofox.model.mhc_parser import MhcParser from neofox.model.validation import InputValidator diff --git a/neofox/tests/integration_tests/test_alternative_mutation.py b/neofox/tests/integration_tests/test_alternative_mutation.py index 3193d5e1..4496b19f 100755 --- a/neofox/tests/integration_tests/test_alternative_mutation.py +++ b/neofox/tests/integration_tests/test_alternative_mutation.py @@ -76,18 +76,18 @@ def test_best_multiple_mhc2_run(self): logger.info(best_multiple.best_predicted_epitope_rank.mutated_peptide) logger.info(best_multiple.best_predicted_epitope_rank.wild_type_peptide) logger.info(best_multiple.phbr_ii) - self.assertEqual(0.8, best_multiple.best_predicted_epitope_rank.rank_mutated) + self.assertEqual(0.01, best_multiple.best_predicted_epitope_rank.rank_mutated) self.assertEqual( - 185.02, best_multiple.best_predicted_epitope_affinity.affinity_mutated + 20.33, best_multiple.best_predicted_epitope_affinity.affinity_mutated ) self.assertEqual( - "VVKWKFMVSTADPGS", best_multiple.best_predicted_epitope_rank.mutated_peptide + "TADPGSFTSRPACSS", best_multiple.best_predicted_epitope_rank.mutated_peptide ) self.assertEqual( - "ITPWRFKLSCMPPNS", best_multiple.best_predicted_epitope_rank.wild_type_peptide + "TADPGAGNPQPGDSS", best_multiple.best_predicted_epitope_rank.wild_type_peptide ) self.assertIsNotNone(best_multiple.phbr_ii) - self.assertAlmostEqual(2.9386450524753664, best_multiple.phbr_ii) + self.assertAlmostEqual(0.10615206237888129, best_multiple.phbr_ii) def test_best_multiple_run(self): best_multiple = BestAndMultipleBinder( diff --git a/neofox/tests/integration_tests/test_best_multiple_binder.py b/neofox/tests/integration_tests/test_best_multiple_binder.py index 99c0b235..73c6d5c6 100755 --- a/neofox/tests/integration_tests/test_best_multiple_binder.py +++ b/neofox/tests/integration_tests/test_best_multiple_binder.py @@ -166,12 +166,12 @@ def test_best_multiple_mhc2_run(self): logger.info(best_multiple.best_predicted_epitope_rank) logger.info(best_multiple.best_predicted_epitope_affinity) logger.info(best_multiple.phbr_ii) - self.assertEqual(3.26, best_multiple.best_predicted_epitope_rank.rank_mutated) + self.assertEqual(1.81, best_multiple.best_predicted_epitope_rank.rank_mutated) self.assertEqual( - 1103.46, best_multiple.best_predicted_epitope_affinity.affinity_mutated + 925.14, best_multiple.best_predicted_epitope_affinity.affinity_mutated ) self.assertEqual( - "SQDILVTDQTRLEAT", best_multiple.best_predicted_epitope_rank.mutated_peptide + "VTDQTRLEATISPET", best_multiple.best_predicted_epitope_rank.mutated_peptide ) def test_phbr2(self): @@ -206,7 +206,7 @@ def test_phbr2(self): ) phbr_ii = best_multiple.calculate_phbr_ii(best_predicted_epitopes_per_alelle) self.assertIsNotNone(phbr_ii) - self.assertAlmostEqual(8.895757526065129, phbr_ii) + self.assertAlmostEqual(6.003569784696215, phbr_ii) # mo info for one allele mhc2_alleles = MhcFactory.build_mhc2_alleles( [ @@ -346,5 +346,5 @@ def test_generator_rate_mhcII(self): generator_rate_ADN = best_multiple.determine_number_of_alternative_binders(predictions=paired_predictions) generator_rate_CDN = best_multiple.determine_number_of_binders(predictions=paired_predictions) - self.assertEqual(generator_rate_ADN, 0) + self.assertEqual(generator_rate_ADN, 1) self.assertEqual(generator_rate_CDN, 0) diff --git a/neofox/tests/integration_tests/test_neofox.py b/neofox/tests/integration_tests/test_neofox.py index 76a18bb9..2973ad0b 100755 --- a/neofox/tests/integration_tests/test_neofox.py +++ b/neofox/tests/integration_tests/test_neofox.py @@ -20,7 +20,7 @@ from unittest import TestCase from datetime import datetime import pkg_resources -import orjson as json +import json from neofox.exceptions import NeofoxConfigurationException from neofox import NEOFOX_MIXMHCPRED_ENV, NEOFOX_MIXMHC2PRED_ENV, NEOFOX_PRIME_ENV @@ -135,7 +135,7 @@ def test_neofox(self): ModelConverter.annotations2neoantigens_table(neoantigens=annotations).to_csv( output_file, sep="\t", index=False) - with open(output_json_neoantigens, "wb") as f: + with open(output_json_neoantigens, "w") as f: f.write(json.dumps(ModelConverter.objects2json(annotations))) # regression test @@ -171,7 +171,7 @@ def test_neomouse(self): ModelConverter.annotations2neoantigens_table(neoantigens=annotations).to_csv( output_file, sep="\t", index=False) - 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">=3.7.1,<3.9" +content-hash = "a76af5d0ef601ea1dcd8eb6eeb37e93c3fb3164b1cfcfba7254fe648023b5e18" diff --git a/pyproject.toml b/pyproject.toml new file mode 100644 index 00000000..d14add93 --- /dev/null +++ b/pyproject.toml @@ -0,0 +1,54 @@ +[tool.poetry] +name = "neofox" +version = "1.1.1.dev8" +description = "NEOantigen Feature tOolboX" +authors = [ + "TRON - Translational Oncology at the University Medical Center", + "of the Johannes Gutenberg University Mainz - ", + "Computational Medicine group ", + "" +] +license = "GPLv3" +readme = "README.md" +packages = [ + {include = "neofox"} +] + +# main dependency group +[tool.poetry.dependencies] +python = ">=3.7.1,<3.9" +biopython = "1.76" +mock = "~4.0.3" +pandas = ">=1.3.5,<1.4" +numpy = ">=1.21,<1.22" +scipy = ">=1.7.3,<1.8" +scikit-learn = "0.22.1" +logzero = ">=1.5.0" +python-dotenv = "~0.12.0" +betterproto = "~1.2.5" +pysam = "~0.19.1" +dask = ">=2022.2.0,<2023.0.0" +distributed = ">=2022.2.0,<2023.0.0" +faker = "~13.13.0" +xmltodict = "~0.12.0" +cython = "0.29.33" + +#[tool.poetry.group.doc] +#optional = true +# +#[tool.poetry.group.doc.dependencies] +#Sphinx = "*"#">6.0.0,<=7.1.2" +#docutils = "*"#"~0.20.1" +#m2r2 = "0.3.3"#">0.3.0" +#nbsphinx = "*"#">0.8.0" +#pydata-sphinx-theme = "*" +#pandoc = "*" + +[tool.poetry.scripts] +neofox = "neofox.command_line:neofox_cli" +neofox-epitope = "neofox.command_line:neofox_epitope_cli" +neofox-configure = "neofox.command_line:neofox_configure" + +[build-system] +requires = ["poetry-core"] +build-backend = "poetry.core.masonry.api" From f51bdbd672062c678269c24bf1c035b248ddb74d Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Mon, 25 Mar 2024 13:17:03 +0100 Subject: [PATCH 37/66] Update documentation for poetry installation. Removed setuptools related files --- MANIFEST.in | 11 ----- docs/source/04_developer_guide.md | 55 +++++++++++++++++++++--- requirements.txt | 15 ------- setup.cfg | 2 - setup.py | 71 ------------------------------- tox.ini | 10 ----- 6 files changed, 49 insertions(+), 115 deletions(-) delete mode 100755 MANIFEST.in delete mode 100755 requirements.txt delete mode 100755 setup.cfg delete mode 100755 setup.py delete mode 100755 tox.ini diff --git a/MANIFEST.in b/MANIFEST.in deleted file mode 100755 index 988c5cb8..00000000 --- a/MANIFEST.in +++ /dev/null @@ -1,11 +0,0 @@ -include neofox/published_features/self_similarity/BLOSUM62-2.matrix.txt -include neofox/published_features/Tcell_predictor/amino-acids-features.pickle -include neofox/published_features/Tcell_predictor/genes-expression.pickle -include neofox/published_features/Tcell_predictor/SIRdata.mat -include neofox/published_features/Tcell_predictor/Classifier.pickle -include neofox/references/h2_database_allele_list.csv -include neofox/expression_imputation/tcga_cohort_code.tab -include neofox/expression_imputation/tcga_exp_summary_modified.tab.gz.tbi -include neofox/expression_imputation/tcga_exp_summary_modified.tab.gz -include requirements.txt -include README.md diff --git a/docs/source/04_developer_guide.md b/docs/source/04_developer_guide.md index baa65671..c521c1b9 100644 --- a/docs/source/04_developer_guide.md +++ b/docs/source/04_developer_guide.md @@ -1,17 +1,33 @@ # Developer guide -## Build the package +> Information: The build system changed in version 1.1.1 to poetry (https://python-poetry.org/). + +## Installation of neofox via poetry + +First setup poetry (see ![Setup poetry](setup-poetry)). + +To install neofox into the virtual environment managed by poetry (if a `poetry.lock` file is given, the given versions are used. Otherwise the dependecies listed in pyproject.toml are used to generate the environment): + +``` +poetry install +``` + +If the **wheel** archives are needed, they can be generated using (generates them in the `dist` directory): -To build the package just run: ``` -python setup.py bdist_wheel +poetry build ``` -This will create an installable wheel file under `dist/neofox-x.y.z.whl`. +To use the installed package use `poetry run` e.g.: -## Install the package +``` +poetry run neofox --help +``` + +## Installation via pip + +The wheel file has to be generated as described in ![Installation of neofox via poetry](installation-of-neofox-via-poetry) (`poetry build`). -Install the wheel file as follows: ``` pip install dist/neofox-x.y.z.whl ``` @@ -62,3 +78,30 @@ The models and the required scripts are in the folder `neofox/models` Build the models into Python code with `make models`. Build the HTML documentation with `make html` (this requires docker). + +## Setup poetry + +Use mamba to install poetry + +``` +mamba create -n poetry conda-forge::poetry +``` + +Required python version for the neofox package also installed via mamba + +``` +# installs python v3.7.12 +mamba create -n python3.7 conda-forge::python=3.7 +``` + +Set the python executable for poetry. This generates the python venv `~/.cache/pypoetry/virtualenvs/neofox...`. + +``` +poetry env use ~/.conda/envs/mamba/envs/python3.7/bin/python3.7 +``` + +To get information on the currently used python environment variable run. This will also show the location of the virtual environment in which the package is or will be installed. + +``` +poetry env info +``` diff --git a/requirements.txt b/requirements.txt deleted file mode 100755 index ad19e827..00000000 --- a/requirements.txt +++ /dev/null @@ -1,15 +0,0 @@ -biopython==1.76 -mock~=4.0.3 -pandas>=1.3.5,<1.4 -numpy>=1.21,<1.22 -scipy>=1.7.3,<1.8 -scikit-learn==0.22.1 -logzero>=1.5.0 -python-dotenv~=0.12.0 -betterproto~=1.2.5 -pysam~=0.19.1 -dask[complete]>=2022.2.0,<2023.0.0 -distributed>=2022.2.0,<2023.0.0 -faker~=13.13.0 -xmltodict~=0.12.0 -cython==0.29.33 diff --git a/setup.cfg b/setup.cfg deleted file mode 100755 index a2f3748e..00000000 --- a/setup.cfg +++ /dev/null @@ -1,2 +0,0 @@ -[metadata] -description-file = README.md \ No newline at end of file diff --git a/setup.py b/setup.py deleted file mode 100755 index 7643d65f..00000000 --- a/setup.py +++ /dev/null @@ -1,71 +0,0 @@ -# -# Copyright (c) 2020-2030 Translational Oncology at the Medical Center of the Johannes Gutenberg-University Mainz gGmbH. -# -# This file is part of Neofox -# (see https://github.com/tron-bioinformatics/neofox). -# -# This program is free software: you can redistribute it and/or modify -# it under the terms of the GNU General Public License as published by -# the Free Software Foundation, either version 3 of the License, or -# (at your option) any later version. -# -# This program is distributed in the hope that it will be useful, -# but WITHOUT ANY WARRANTY; without even the implied warranty of -# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the -# GNU General Public License for more details. -# -# You should have received a copy of the GNU General Public License -# along with this program. If not, see .# -from setuptools import find_packages, setup, Command -import neofox - - -# parses requirements from file -with open("requirements.txt", encoding="utf-8") as f: - required = f.read().splitlines() - -with open("README.md", "r", encoding="utf-8") as f: - long_description = f.read() - -# Build the Python package -setup( - name="neofox", - version=neofox.VERSION, - packages=find_packages(), - include_package_data=True, - entry_points={ - "console_scripts": [ - "neofox=neofox.command_line:neofox_cli", - "neofox-epitope=neofox.command_line:neofox_epitope_cli", - "neofox-configure=neofox.command_line:neofox_configure", - ], - }, - author_email="franziska.lang@tron-mainz.de", - author="TRON - Translational Oncology at the University Medical Center of the Johannes Gutenberg University Mainz " - "- Computational Medicine group", - description="Annotation of mutated peptide sequences (mps) with published or novel potential neo-epitope " - "descriptors", - long_description=long_description, - long_description_content_type="text/markdown", - url="https://github.com/tron-bioinformatics/neofox", - requires=[], - # NOTE: always specify versions to ensure build reproducibility - # NOTE2: sklearn==0.19.0 is a hidden dependency as it is required by Classifier.pickle - install_requires=required, - setup_requires=[], - classifiers=[ - "Development Status :: 5 - Production/Stable", # Chose either "3 - Alpha", "4 - Beta" or "5 - Production/Stable" as the current state of your package - "Intended Audience :: Healthcare Industry", - "Intended Audience :: Science/Research", - "Topic :: Scientific/Engineering :: Bio-Informatics", - "Programming Language :: Python", - "Programming Language :: Python :: 3", - 'Programming Language :: Python :: 3.7', - 'Programming Language :: Python :: 3.8', - "Programming Language :: Python :: 3 :: Only", - "License :: OSI Approved :: GNU General Public License v3 (GPLv3)", - "Operating System :: Unix" - ], - python_requires='>=3.6,<3.9', - license='GPLv3', -) diff --git a/tox.ini b/tox.ini deleted file mode 100755 index 56e569c4..00000000 --- a/tox.ini +++ /dev/null @@ -1,10 +0,0 @@ -[tox] -envlist = {py37, py38} - -[testenv] -wheel = true -passenv = * -commands= - pip install -r requirements.txt - pip freeze - python -m unittest discover neofox.tests.unit_tests \ No newline at end of file From 58cc8a0423f111eda5dbf99eb4fac0c37a55ee97 Mon Sep 17 00:00:00 2001 From: "Kress, Luis" Date: Mon, 25 Mar 2024 13:24:27 +0100 Subject: [PATCH 38/66] Update of developer guide to contain crosslinks --- docs/source/04_developer_guide.md | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/docs/source/04_developer_guide.md b/docs/source/04_developer_guide.md index c521c1b9..2f918ce9 100644 --- a/docs/source/04_developer_guide.md +++ b/docs/source/04_developer_guide.md @@ -4,7 +4,7 @@ ## Installation of neofox via poetry -First setup poetry (see ![Setup poetry](setup-poetry)). +First setup poetry (see [Setup poetry](setup-poetry)). To install neofox into the virtual environment managed by poetry (if a `poetry.lock` file is given, the given versions are used. Otherwise the dependecies listed in pyproject.toml are used to generate the environment): @@ -12,7 +12,7 @@ To install neofox into the virtual environment managed by poetry (if a `poetry.l poetry install ``` -If the **wheel** archives are needed, they can be generated using (generates them in the `dist` directory): +If the **wheel** archive is needed, they can be generated using (generates them in the `dist` directory): ``` poetry build @@ -26,7 +26,7 @@ poetry run neofox --help ## Installation via pip -The wheel file has to be generated as described in ![Installation of neofox via poetry](installation-of-neofox-via-poetry) (`poetry build`). +The wheel file has to be generated as described in [Installation of neofox via poetry](installation-of-neofox-via-poetry) (`poetry build`). ``` pip install dist/neofox-x.y.z.whl From e244b2837485b287e639a1fb49af052ca3fc6845 Mon Sep 17 00:00:00 2001 From: "Kress, Luis" Date: Mon, 25 Mar 2024 13:26:03 +0100 Subject: [PATCH 39/66] Make crosslinks in developer guide clickable. --- docs/source/04_developer_guide.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/docs/source/04_developer_guide.md b/docs/source/04_developer_guide.md index 2f918ce9..74657cbb 100644 --- a/docs/source/04_developer_guide.md +++ b/docs/source/04_developer_guide.md @@ -4,7 +4,7 @@ ## Installation of neofox via poetry -First setup poetry (see [Setup poetry](setup-poetry)). +First setup poetry (see [Setup poetry](#setup-poetry)). To install neofox into the virtual environment managed by poetry (if a `poetry.lock` file is given, the given versions are used. Otherwise the dependecies listed in pyproject.toml are used to generate the environment): @@ -26,7 +26,7 @@ poetry run neofox --help ## Installation via pip -The wheel file has to be generated as described in [Installation of neofox via poetry](installation-of-neofox-via-poetry) (`poetry build`). +The wheel file has to be generated as described in [Installation of neofox via poetry](#installation-of-neofox-via-poetry) (`poetry build`). ``` pip install dist/neofox-x.y.z.whl From 5de92dd3d5cd3004d7a44ccc27903afea3434b98 Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Mon, 25 Mar 2024 14:14:38 +0100 Subject: [PATCH 40/66] Update gitlab CI --- .gitlab-ci.yml | 4 +++- tox.ini | 9 +++++++++ 2 files changed, 12 insertions(+), 1 deletion(-) create mode 100644 tox.ini diff --git a/.gitlab-ci.yml b/.gitlab-ci.yml index a2e94a0c..8ca12fad 100755 --- a/.gitlab-ci.yml +++ b/.gitlab-ci.yml @@ -37,6 +37,8 @@ before_script: - apt-get update - apt-get --assume-yes install gcc gfortran build-essential wget libfreetype6-dev libpng-dev libopenblas-dev - python -V + - pip install poetry=1.8.2 + - poetry install --only main stages: - validation @@ -63,7 +65,7 @@ publish_package: stage: deploy script: - pip install twine - - python3 setup.py sdist bdist_wheel + - poetry run python3 setup.py sdist bdist_wheel - TWINE_PASSWORD=${CI_JOB_TOKEN} TWINE_USERNAME=gitlab-ci-token python -m twine upload --repository-url https://gitlab.rlp.net/api/v4/projects/${CI_PROJECT_ID}/packages/pypi dist/* only: # deploys in private gitlab package repository only the develop branch, the master branch is published in PyPI diff --git a/tox.ini b/tox.ini new file mode 100644 index 00000000..231af1ea --- /dev/null +++ b/tox.ini @@ -0,0 +1,9 @@ +[tox] +envlist = {py37, py38} + +[testenv] +wheel = true +passenv = * +commands= + poetry run python -m unittest discover neofox.tests.unit_tests + From d64efb7056cb0c7628b663560f045268e46de665 Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Mon, 25 Mar 2024 14:16:55 +0100 Subject: [PATCH 41/66] Update deploy stage gitlab CI poetry --- .gitlab-ci.yml | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/.gitlab-ci.yml b/.gitlab-ci.yml index 8ca12fad..771b57af 100755 --- a/.gitlab-ci.yml +++ b/.gitlab-ci.yml @@ -38,7 +38,7 @@ before_script: - apt-get --assume-yes install gcc gfortran build-essential wget libfreetype6-dev libpng-dev libopenblas-dev - python -V - pip install poetry=1.8.2 - - poetry install --only main + - poetry install stages: - validation @@ -65,7 +65,7 @@ publish_package: stage: deploy script: - pip install twine - - poetry run python3 setup.py sdist bdist_wheel + - poetry build - TWINE_PASSWORD=${CI_JOB_TOKEN} TWINE_USERNAME=gitlab-ci-token python -m twine upload --repository-url https://gitlab.rlp.net/api/v4/projects/${CI_PROJECT_ID}/packages/pypi dist/* only: # deploys in private gitlab package repository only the develop branch, the master branch is published in PyPI From 7fef2a092010dac065fe65bd8d6c56bcafbe963f Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Mon, 25 Mar 2024 14:19:23 +0100 Subject: [PATCH 42/66] Bump version --- neofox/__init__.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/neofox/__init__.py b/neofox/__init__.py index 427faf9d..61755c66 100755 --- a/neofox/__init__.py +++ b/neofox/__init__.py @@ -18,7 +18,7 @@ # along with this program. If not, see .# -VERSION = "1.1.1.dev8" +VERSION = "1.1.1.dev9" REFERENCE_FOLDER_ENV = "NEOFOX_REFERENCE_FOLDER" NEOFOX_BLASTP_ENV = "NEOFOX_BLASTP" From 91f6c96eccf2916040716837d668259470fa8b56 Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Mon, 25 Mar 2024 14:26:02 +0100 Subject: [PATCH 43/66] Use poetry only in test and deploy stage of Gitlab CI --- .gitlab-ci.yml | 3 ++- tox.ini | 1 + 2 files changed, 3 insertions(+), 1 deletion(-) diff --git a/.gitlab-ci.yml b/.gitlab-ci.yml index 771b57af..e9309521 100755 --- a/.gitlab-ci.yml +++ b/.gitlab-ci.yml @@ -38,7 +38,7 @@ before_script: - apt-get --assume-yes install gcc gfortran build-essential wget libfreetype6-dev libpng-dev libopenblas-dev - python -V - pip install poetry=1.8.2 - - poetry install + #- poetry install stages: - validation @@ -65,6 +65,7 @@ publish_package: stage: deploy script: - pip install twine + - poetry install - poetry build - TWINE_PASSWORD=${CI_JOB_TOKEN} TWINE_USERNAME=gitlab-ci-token python -m twine upload --repository-url https://gitlab.rlp.net/api/v4/projects/${CI_PROJECT_ID}/packages/pypi dist/* only: diff --git a/tox.ini b/tox.ini index 231af1ea..80c76b8e 100644 --- a/tox.ini +++ b/tox.ini @@ -5,5 +5,6 @@ envlist = {py37, py38} wheel = true passenv = * commands= + poetry install --only main poetry run python -m unittest discover neofox.tests.unit_tests From 293d1fcc722ad0c84d57fff154f88960d9fa3e2e Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Mon, 25 Mar 2024 14:29:49 +0100 Subject: [PATCH 44/66] Fixed poetry installation version definition in GitLab CI --- .gitlab-ci.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.gitlab-ci.yml b/.gitlab-ci.yml index e9309521..8abcf724 100755 --- a/.gitlab-ci.yml +++ b/.gitlab-ci.yml @@ -37,7 +37,7 @@ before_script: - apt-get update - apt-get --assume-yes install gcc gfortran build-essential wget libfreetype6-dev libpng-dev libopenblas-dev - python -V - - pip install poetry=1.8.2 + - pip install poetry==1.8.2 #- poetry install stages: From cbc4004a455859356bf5c2d032f2454c6320f35c Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Mon, 25 Mar 2024 14:53:10 +0100 Subject: [PATCH 45/66] Update tox.ini to run with poetry --- tox.ini | 5 +++-- 1 file changed, 3 insertions(+), 2 deletions(-) diff --git a/tox.ini b/tox.ini index 80c76b8e..c4f96075 100644 --- a/tox.ini +++ b/tox.ini @@ -1,10 +1,11 @@ [tox] envlist = {py37, py38} +isolated_build = True [testenv] -wheel = true passenv = * -commands= +commands_pre= poetry install --only main +commands= poetry run python -m unittest discover neofox.tests.unit_tests From 2aa2d38b677b8e16437e9f73e3598d0b699af030 Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Mon, 25 Mar 2024 15:31:49 +0100 Subject: [PATCH 46/66] Version information is now only located in pyproject.toml and inferred in __init__.py --- .gitlab-ci.yml | 3 +-- neofox/__init__.py | 9 ++++++++- 2 files changed, 9 insertions(+), 3 deletions(-) diff --git a/.gitlab-ci.yml b/.gitlab-ci.yml index 8abcf724..97dbbbb4 100755 --- a/.gitlab-ci.yml +++ b/.gitlab-ci.yml @@ -38,7 +38,6 @@ before_script: - apt-get --assume-yes install gcc gfortran build-essential wget libfreetype6-dev libpng-dev libopenblas-dev - python -V - pip install poetry==1.8.2 - #- poetry install stages: - validation @@ -50,7 +49,7 @@ check_version_changes: script: # if the version number does not change between this branch and develop it fails - git fetch origin develop - - if git diff origin/develop -- neofox/__init__.py | grep VERSION; then exit 0; else echo "Version needs to be increased!"; exit -1; fi + - if git diff origin/develop -- pyproject.toml | grep version; then exit 0; else echo "Version needs to be increased!"; exit -1; fi except: - develop - master diff --git a/neofox/__init__.py b/neofox/__init__.py index 61755c66..fdc8e7ee 100755 --- a/neofox/__init__.py +++ b/neofox/__init__.py @@ -17,8 +17,15 @@ # You should have received a copy of the GNU General Public License # along with this program. If not, see .# +import platform +from packaging import version -VERSION = "1.1.1.dev9" +if version.parse(platform.python_version()) Date: Thu, 4 Apr 2024 12:28:00 +0200 Subject: [PATCH 47/66] Update GitLab CI --- .github/workflows/release.yml | 7 ++++--- .gitlab-ci.yml | 8 +++++--- 2 files changed, 9 insertions(+), 6 deletions(-) diff --git a/.github/workflows/release.yml b/.github/workflows/release.yml index 78998c9a..82076c31 100755 --- a/.github/workflows/release.yml +++ b/.github/workflows/release.yml @@ -14,11 +14,12 @@ jobs: - name: Install dependencies run: | python -m pip install --upgrade pip - pip install setuptools wheel twine + pip install poetry==1.8.2 twine - name: Build and publish env: TWINE_USERNAME: __token__ TWINE_PASSWORD: ${{ secrets.PYPI_PASSWORD }} run: | - python setup.py sdist bdist_wheel - twine upload dist/* \ No newline at end of file + poetry install + poetry build + twine upload dist/*.whl \ No newline at end of file diff --git a/.gitlab-ci.yml b/.gitlab-ci.yml index 97dbbbb4..810ffcd9 100755 --- a/.gitlab-ci.yml +++ b/.gitlab-ci.yml @@ -50,9 +50,11 @@ check_version_changes: # if the version number does not change between this branch and develop it fails - git fetch origin develop - if git diff origin/develop -- pyproject.toml | grep version; then exit 0; else echo "Version needs to be increased!"; exit -1; fi - except: - - develop - - master + #except: + # - develop + # - master + rules: + - if: $CI_PIPELINE_SOURCE == 'merge_request_event' test: stage: test From d77c06ba91587560eb8ddb33c89bd4d66e3d3160 Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Thu, 4 Apr 2024 15:43:02 +0200 Subject: [PATCH 48/66] Set GitLab CI version check back to original state --- .gitlab-ci.yml | 8 +++----- 1 file changed, 3 insertions(+), 5 deletions(-) diff --git a/.gitlab-ci.yml b/.gitlab-ci.yml index 810ffcd9..97dbbbb4 100755 --- a/.gitlab-ci.yml +++ b/.gitlab-ci.yml @@ -50,11 +50,9 @@ check_version_changes: # if the version number does not change between this branch and develop it fails - git fetch origin develop - if git diff origin/develop -- pyproject.toml | grep version; then exit 0; else echo "Version needs to be increased!"; exit -1; fi - #except: - # - develop - # - master - rules: - - if: $CI_PIPELINE_SOURCE == 'merge_request_event' + except: + - develop + - master test: stage: test From 13537334f08ffa27320d285687a43bcc4073e26b Mon Sep 17 00:00:00 2001 From: Franziska Lang Date: Fri, 5 Apr 2024 12:50:40 +0200 Subject: [PATCH 49/66] update documentation on poetry --- docs/source/04_developer_guide.md | 52 ++++++++++++++++++++----------- 1 file changed, 34 insertions(+), 18 deletions(-) diff --git a/docs/source/04_developer_guide.md b/docs/source/04_developer_guide.md index 74657cbb..c4c3aa84 100644 --- a/docs/source/04_developer_guide.md +++ b/docs/source/04_developer_guide.md @@ -4,38 +4,48 @@ ## Installation of neofox via poetry -First setup poetry (see [Setup poetry](#setup-poetry)). +Setup poetry once as described under [Setup poetry](#setup-poetry)) . -To install neofox into the virtual environment managed by poetry (if a `poetry.lock` file is given, the given versions are used. Otherwise the dependecies listed in pyproject.toml are used to generate the environment): +Go to your local repository, add poetry to you path and install neofox into a virtual environment managed by poetry. If a `poetry.lock` file is given in the path, herein defined package versions are used. Otherwise the dependencies listed in pyproject.toml are used to generate the environment: ``` -poetry install +cd /path/to/neofox/addannot + +export PATH="$PATH:/path/to/env/poetry/bin/" + +poetry install ``` -If the **wheel** archive is needed, they can be generated using (generates them in the `dist` directory): +If a **wheel** archive is needed, it can be generated optionally using (generates them in the `dist` directory): ``` -poetry build + ~/.conda/envs/poetry/bin/poetry build ``` -To use the installed package use `poetry run` e.g.: +To use the installed neofox package, use `poetry run` e.g.: ``` -poetry run neofox --help + ~/.conda/envs/poetry/bin/poetry run neofox --help ``` ## Installation via pip -The wheel file has to be generated as described in [Installation of neofox via poetry](#installation-of-neofox-via-poetry) (`poetry build`). +If a wheel file was generated with `poetry build` [Installation of neofox via poetry](#installation-of-neofox-via-poetry), neofox can also be installed via pip. ``` pip install dist/neofox-x.y.z.whl ``` -## Run integration tests +## Testing -To run the integration tests make sure you have a file `.env` that contains the environment variables described in the configuration section. +To run the tests make sure you have a file `.env` that contains the environment variables described in the configuration section. + +Activate the virtual environment generated by poetry: +``` +source .venv/bin/activate +``` +### Run integration tests Run the integration tests as follows: ``` python -m unittest discover neofox.tests.integration_tests @@ -52,7 +62,7 @@ python -m unittest neofox.tests.integration_tests.test_neofox This last test (ie: `test_neofox`) writes its output to a file named `neofox/tests/resources/output_yyyymmddHHMMSS.txt`. If there is an existing file named `neofox/tests/resources/output_previous.txt` then it loads both files in memory and compares them. It outputs whether there are some lost or gained columns and for the common columns it evaluates if the values are the same. If they are the same the file `output_previous.txt` is overwritten by the new file, otherwise it outputs the details of the differing columns. -## Run unit tests +### Run unit tests The unit tests do not have any dependency and they finish in seconds. @@ -81,26 +91,32 @@ Build the HTML documentation with `make html` (this requires docker). ## Setup poetry -Use mamba to install poetry +Setup poetry once on your system. + +E.g. use mamba to install poetry: ``` -mamba create -n poetry conda-forge::poetry +mamba create -p /path/to/env/poetry conda-forge::poetry ``` -Required python version for the neofox package also installed via mamba +The required python version for the neofox package can also be installed via mamba (currently python3.7): ``` # installs python v3.7.12 -mamba create -n python3.7 conda-forge::python=3.7 +mamba create -p /path/to/env/python3.7 conda-forge::python=3.7 ``` -Set the python executable for poetry. This generates the python venv `~/.cache/pypoetry/virtualenvs/neofox...`. +Go to your local neofox repository and set the python executable for poetry. This generates the python venv `/path/to/neofox/addannot/.venv`. ``` -poetry env use ~/.conda/envs/mamba/envs/python3.7/bin/python3.7 +export PATH="$PATH:/path/to/env/poetry/bin/" + +cd /path/to/neofox/addannot + +poetry env use /path/to/env/python3.7/bin/python3.7 ``` -To get information on the currently used python environment variable run. This will also show the location of the virtual environment in which the package is or will be installed. +Get information on the currently used python environment variable by running the following command. This will also show the location of the virtual environment in which the package is or will be installed. ``` poetry env info From a6b9d2abd7ac2a0adbcb35be628ad227685aba63 Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Mon, 8 Apr 2024 11:45:46 +0200 Subject: [PATCH 50/66] Updated README and removed home paths from developer guide --- README.md | 35 ++++++++++++++++++++----------- docs/source/04_developer_guide.md | 4 ++-- 2 files changed, 25 insertions(+), 14 deletions(-) diff --git a/README.md b/README.md index 4c453ecf..8f53aa00 100755 --- a/README.md +++ b/README.md @@ -75,21 +75,32 @@ conda install bioconda::neofox NeoFox can be used from the command line as shown below or programmatically (see [https://neofox.readthedocs.io](https://neofox.readthedocs.io/) for more information). ````commandline -neofox --candidate-file/--json-file neoantigens_candidates.tab/neoantigens_candidates.json --patient-data/--patient-data-json patient_data.txt/patient_data.json --output-folder /path/to/out --output-prefix out_prefix [--patient-id] [--with-table] [--with-json] [--num-cpus] [--affinity-threshold] +neofox --input-file neoantigens_candidates.tsv \ + --patient-data patient_data.txt \ + --output-folder /path/to/out \ + [--output-prefix out_prefix] \ + [--organism human|mouse] \ + [--rank-mhci-threshold 2.0] \ + [--rank-mhcii-threshold 5.0] \ + [--num-cpus] \ + [--config] \ + [--patient-id] \ + [--with-all-neoepitopes] \ + [--verbose] ```` -- `--candidate-file`: tab-separated values table with neoantigen candidates represented by long mutated peptide sequences as described [here](#41-neoantigen-candidates-in-tabular-format) -- `--json-file`: JSON file neoantigens in NeoFox model format as described [here](#42-neoantigen-candidates-in-json-format) -- `--patient-id`: patient identifier (*optional*, this will be used if the column `patientIdentifier` is missing in the candidate input file) -- `--patient-data`: a table of tab separated values containing metadata on the patient as described [here](#43-patient-data-format) +- `--input-file`: tab-separated values table with neoantigen candidates represented by long mutated peptide sequences + as described [here](03_01_input_data.md#tabular-file-format) (extensions .txt and .tsv) or JSON file neoantigens in + NeoFox model format as described [here](03_01_input_data.md#json-file-format) (extension .json) +- `--patient-data`: a table of tab separated values containing metadata on the patient as described [here](03_01_input_data.md#file-with-patient-information) - `--output-folder`: path to the folder to which the output files should be written - `--output-prefix`: prefix for the output files (*optional*) -- `--with-table`: output file in tab-separated format (*default*) -- `--with-json`: output file in JSON format (*optional*) +- `--with-all-neoepitopes`: output annotations for all MHC-I and MHC-II neoepitopes on all HLA alleles (*optional*) +- `--rank-mhci-threshold`: MHC-I epitopes with a netMHCpan predicted rank greater than or equal than this threshold will be filtered out (*optional*) +- `--rank-mhcii-threshold`: MHC-II epitopes with a netMHCIIpan predicted rank greater than or equal than this threshold will be filtered out (*optional*) +- `--organism`: the organism to which the data corresponds. Possible values: [human, mouse]. Default value: human - `--num-cpus`: number of CPUs to use (*optional*) - `--config`: a config file with the paths to dependencies as shown below (*optional*) -- `--organism`: the organism to which the data corresponds. Possible values: [human, mouse]. Default value: human -- `--affinity-threshold`: a affinity value (*optional*) neoantigen candidates with a best predicted affinity greater than or equal than this threshold will be not annotated with features that specifically model - neoepitope recognition. A threshold that is commonly used is 500 nM. +- `--patient-id`: patient identifier (*optional*, this is only relevant if the column `patientIdentifier` is missing in the candidate input file) - `--verbose`: get detailed logs @@ -157,9 +168,9 @@ where: - `identifier`: the patient identifier - `mhcIAlleles`: comma separated MHC I alleles of the patient for HLA-A, HLA-B and HLA-C. If homozygous, the allele should be added twice. - `mhcIIAlleles`: comma separated MHC II alleles of the patient for HLA-DRB1, HLA-DQA1, HLA-DQB1, HLA-DPA1 and HLA-DPB1. If homozygous, the allele should be added twice. -- `tumorType`: tumour entity in TCGA study abbreviation format (https://gdc.cancer.gov/resources-tcga-users/tcga-code-tables/tcga-study-abbreviations). This field is required for expression imputation and at the moment the following tumor types are supported: +- `tumorType`: tumour entity in TCGA study abbreviation format (https://gdc.cancer.gov/resources-tcga-users/tcga-code-tables/tcga-study-abbreviations). This field is required for expression imputation. The supported tumor types are listed under "Input data" in the documentation. ## 5 Output data -The output data is returned by default in a short wide tab separated values file (`--with-table`). Optionally, it can be provided in JSON format (`--with-json`). +The output data is returned by default in tsv and json format. With the command line flag `--with-all-neoepitopes`, two additional files are generated containing the epitope candidates for MHCI and MHCII with NetMHCpan predictions below the given thresholds. diff --git a/docs/source/04_developer_guide.md b/docs/source/04_developer_guide.md index c4c3aa84..17699dc0 100644 --- a/docs/source/04_developer_guide.md +++ b/docs/source/04_developer_guide.md @@ -19,13 +19,13 @@ poetry install If a **wheel** archive is needed, it can be generated optionally using (generates them in the `dist` directory): ``` - ~/.conda/envs/poetry/bin/poetry build +poetry build ``` To use the installed neofox package, use `poetry run` e.g.: ``` - ~/.conda/envs/poetry/bin/poetry run neofox --help +poetry run neofox --help ``` ## Installation via pip From ec54e5c919b46b229d754a4b0462925788c29386 Mon Sep 17 00:00:00 2001 From: Luis Kress Date: Mon, 8 Apr 2024 11:58:06 +0200 Subject: [PATCH 51/66] Added poetry instruction to developer guide to install virtual environments in current project --- docs/source/04_developer_guide.md | 3 +++ 1 file changed, 3 insertions(+) diff --git a/docs/source/04_developer_guide.md b/docs/source/04_developer_guide.md index 17699dc0..1dafd8ed 100644 --- a/docs/source/04_developer_guide.md +++ b/docs/source/04_developer_guide.md @@ -113,6 +113,9 @@ export PATH="$PATH:/path/to/env/poetry/bin/" cd /path/to/neofox/addannot +# environments are installed in the current project +poetry config virtualenvs.in-project true + poetry env use /path/to/env/python3.7/bin/python3.7 ``` From f61505a4b14d59a13247fa1d44d3244e95c9b1b7 Mon Sep 17 00:00:00 2001 From: "Kress, Luis" Date: Wed, 3 Jul 2024 15:37:26 +0200 Subject: [PATCH 52/66] Fixed error in dissimilarity test --- .gitlab-ci.yml | 2 +- README.md | 2 +- docs/source/02_installation.md | 9 +- docs/source/04_developer_guide.md | 6 +- .../netmhcpan/netmhcIIpan_prediction.py | 6 + .../netmhcpan/netmhcpan_prediction.py | 6 + neofox/annotator/abstract_annotator.py | 6 - neofox/annotator/neoantigen_annotator.py | 13 +- neofox/annotator/neoepitope_annotator.py | 5 +- .../neoepitope_mhc_binding_annotator.py | 48 +- neofox/helpers/blastp_runner.py | 21 +- neofox/helpers/epitope_helper.py | 12 +- neofox/model/validation.py | 3 +- neofox/neofox.py | 8 - neofox/neofox_epitope.py | 9 - .../Tcell_predictor/Classifier.pickle | Bin 12320161 -> 0 bytes .../Tcell_predictor/SIRdata.mat | Bin 51269 -> 0 bytes .../Tcell_predictor/__init__.py | 18 - .../amino-acids-features.pickle | Bin 768 -> 0 bytes .../Tcell_predictor/genes-expression.pickle | Bin 470308 -> 0 bytes .../Tcell_predictor/preprocess.py | 151 --- .../Tcell_predictor/tcellpredictor_wrapper.py | 105 -- neofox/published_features/hex/pyhex.py | 4 +- neofox/published_features/priority_score.py | 67 +- .../self_similarity/self_similarity.py | 36 +- neofox/references/installer.py | 5 +- .../integration_test_tools.py | 3 +- .../integration_tests/test_dissimilarity.py | 3 +- .../test_neoantigen_annotator.py | 16 +- .../test_neoepitope_annotator.py | 18 +- neofox/tests/integration_tests/test_neofox.py | 6 +- neofox/tests/integration_tests/test_prime.py | 6 +- .../resources/test_data_model_realistic.txt | 22 +- neofox/tests/synthetic_data/factories.py | 2 +- .../unit_tests/test_differential_binding.py | 6 + neofox/tests/unit_tests/test_runner.py | 5 +- .../tests/unit_tests/test_tcell_predictor.py | 83 -- poetry.lock | 909 +++++++++--------- pyproject.toml | 21 +- tox.ini | 2 +- 40 files changed, 653 insertions(+), 991 deletions(-) delete mode 100755 neofox/published_features/Tcell_predictor/Classifier.pickle delete mode 100755 neofox/published_features/Tcell_predictor/SIRdata.mat delete mode 100755 neofox/published_features/Tcell_predictor/__init__.py delete mode 100755 neofox/published_features/Tcell_predictor/amino-acids-features.pickle delete mode 100755 neofox/published_features/Tcell_predictor/genes-expression.pickle delete mode 100755 neofox/published_features/Tcell_predictor/preprocess.py delete mode 100755 neofox/published_features/Tcell_predictor/tcellpredictor_wrapper.py delete mode 100755 neofox/tests/unit_tests/test_tcell_predictor.py diff --git a/.gitlab-ci.yml b/.gitlab-ci.yml index 97dbbbb4..f4bdc09a 100755 --- a/.gitlab-ci.yml +++ b/.gitlab-ci.yml @@ -18,7 +18,7 @@ ## along with this program. If not, see .## # this image contains multiple Python interpreters -image: python:3.8.14-buster +image: python:3.11.9-bookworm # Change pip's cache directory to be inside the project directory since we can # only cache local items. diff --git a/README.md b/README.md index 8f53aa00..9f9cac80 100755 --- a/README.md +++ b/README.md @@ -168,7 +168,7 @@ where: - `identifier`: the patient identifier - `mhcIAlleles`: comma separated MHC I alleles of the patient for HLA-A, HLA-B and HLA-C. If homozygous, the allele should be added twice. - `mhcIIAlleles`: comma separated MHC II alleles of the patient for HLA-DRB1, HLA-DQA1, HLA-DQB1, HLA-DPA1 and HLA-DPB1. If homozygous, the allele should be added twice. -- `tumorType`: tumour entity in TCGA study abbreviation format (https://gdc.cancer.gov/resources-tcga-users/tcga-code-tables/tcga-study-abbreviations). This field is required for expression imputation. The supported tumor types are listed under "Input data" in the documentation. +- `tumorType`: tumour entity in TCGA study abbreviation format (https://gdc.cancer.gov/resources-tcga-users/tcga-code-tables/tcga-study-abbreviations). This field is required for expression imputation. The supported tumor types are listed under "Input data" in the [documentation](https://neofox.readthedocs.io/en/latest/03_01_input_data.html). ## 5 Output data diff --git a/docs/source/02_installation.md b/docs/source/02_installation.md index 7dc9ebf8..fe53a2ac 100755 --- a/docs/source/02_installation.md +++ b/docs/source/02_installation.md @@ -1,8 +1,9 @@ # Installation This guide contains two alternatives to install NeoFox: +- A set of detailed step by step installation instructions without docker with bioconda or PyPI - Building a docker image that automates the installation into a container (**NOTE**: the docker recipe is not supported in neofox-v1.1.0. Please use an older version (=3.9,<3.12) should be preinstalled. The libz compression development library is required. This can be installed in Ubuntu as follows: ``` @@ -56,7 +57,7 @@ conda install bioconda::neofox ### Install third-party dependencies -**NOTE**: Please, check the licences of third-party dependencies. +**NOTE: Please, check the licences of third-party dependencies.** #### Install BLASTP diff --git a/docs/source/04_developer_guide.md b/docs/source/04_developer_guide.md index 1dafd8ed..dd881bcc 100644 --- a/docs/source/04_developer_guide.md +++ b/docs/source/04_developer_guide.md @@ -102,8 +102,8 @@ mamba create -p /path/to/env/poetry conda-forge::poetry The required python version for the neofox package can also be installed via mamba (currently python3.7): ``` -# installs python v3.7.12 -mamba create -p /path/to/env/python3.7 conda-forge::python=3.7 +# installs python v3.11 +mamba create -p /path/to/env/python3.11 conda-forge::python=3.11 ``` Go to your local neofox repository and set the python executable for poetry. This generates the python venv `/path/to/neofox/addannot/.venv`. @@ -116,7 +116,7 @@ cd /path/to/neofox/addannot # environments are installed in the current project poetry config virtualenvs.in-project true -poetry env use /path/to/env/python3.7/bin/python3.7 +poetry env use /path/to/env/python3.11/bin/python3.11 ``` Get information on the currently used python environment variable by running the following command. This will also show the location of the virtual environment in which the package is or will be installed. diff --git a/neofox/MHC_predictors/netmhcpan/netmhcIIpan_prediction.py b/neofox/MHC_predictors/netmhcpan/netmhcIIpan_prediction.py index 4c0b715c..8a72556f 100755 --- a/neofox/MHC_predictors/netmhcpan/netmhcIIpan_prediction.py +++ b/neofox/MHC_predictors/netmhcpan/netmhcIIpan_prediction.py @@ -152,6 +152,12 @@ def set_wt_netmhcpan_scores(self, predictions) -> List[PredictedEpitope]: # NOTE: netmhcpan in peptide mode should return only one epitope p.rank_wild_type = wt_prediction.rank_mutated p.affinity_wild_type = wt_prediction.affinity_mutated + else: + p.rank_wild_type = None + p.affinity_wild_type = None + else: + p.rank_wild_type = None + p.affinity_wild_type = None return predictions def get_wt_predictions(self, neoantigen: Neoantigen, patient_mhc2_isoforms): diff --git a/neofox/MHC_predictors/netmhcpan/netmhcpan_prediction.py b/neofox/MHC_predictors/netmhcpan/netmhcpan_prediction.py index d7934344..dcd52067 100755 --- a/neofox/MHC_predictors/netmhcpan/netmhcpan_prediction.py +++ b/neofox/MHC_predictors/netmhcpan/netmhcpan_prediction.py @@ -162,6 +162,12 @@ def set_wt_netmhcpan_scores(self, predictions) -> List[PredictedEpitope]: # NOTE: netmhcpan in peptide mode should return only one epitope p.rank_wild_type = wt_prediction.rank_mutated p.affinity_wild_type = wt_prediction.affinity_mutated + else: + p.rank_wild_type = None + p.affinity_wild_type = None + else: + p.rank_wild_type = None + p.affinity_wild_type = None return predictions def get_predictions(self, available_alleles, neoantigen: Neoantigen, uniprot) -> List[PredictedEpitope]: diff --git a/neofox/annotator/abstract_annotator.py b/neofox/annotator/abstract_annotator.py index 5f0307ee..66f32666 100644 --- a/neofox/annotator/abstract_annotator.py +++ b/neofox/annotator/abstract_annotator.py @@ -7,7 +7,6 @@ from neofox.helpers.runner import Runner from neofox.model.factories import AnnotationFactory from neofox.model.neoantigen import PredictedEpitope, Neoantigen, Patient -from neofox.published_features.Tcell_predictor.tcellpredictor_wrapper import TcellPrediction from neofox.published_features.differential_binding.amplitude import Amplitude from neofox.published_features.differential_binding.differential_binding import DifferentialBinding from neofox.published_features.dissimilarity_garnish.dissimilaritycalculator import DissimilarityCalculator @@ -25,13 +24,11 @@ def __init__( self, references: ReferenceFolder, configuration: DependenciesConfiguration, - tcell_predictor: TcellPrediction, self_similarity: SelfSimilarityCalculator ): """class to annotate neoantigens""" self.runner = Runner() self.configuration = configuration - self.tcell_predictor = tcell_predictor self.self_similarity = self_similarity self.organism = references.organism @@ -85,9 +82,6 @@ def get_additional_annotations_neoepitope_mhci( self.dissimilarity_calculator.get_annotations_epitope(epitope=epitope) ) - epitope.neofox_annotations.annotations.extend(self.tcell_predictor.get_annotations_epitope_mhci( - epitope=epitope, gene=gene)) - num_mismatches = EpitopeHelper.number_of_mismatches( epitope_wild_type=epitope.wild_type_peptide, epitope_mutation=epitope.mutated_peptide, ) epitope.neofox_annotations.annotations.append(AnnotationFactory.build_annotation( diff --git a/neofox/annotator/neoantigen_annotator.py b/neofox/annotator/neoantigen_annotator.py index 398cc8fc..ab0e70d7 100755 --- a/neofox/annotator/neoantigen_annotator.py +++ b/neofox/annotator/neoantigen_annotator.py @@ -27,7 +27,6 @@ from neofox.annotator.neoantigen_mhc_binding_annotator import NeoantigenMhcBindingAnnotator from neofox.model.factories import AnnotationFactory from neofox.model.mhc_parser import MhcParser -from neofox.published_features.Tcell_predictor.tcellpredictor_wrapper import TcellPrediction from neofox.published_features.self_similarity.self_similarity import SelfSimilarityCalculator from neofox.published_features.expression import Expression from neofox.model.neoantigen import Patient, Neoantigen, Annotations @@ -41,12 +40,12 @@ class NeoantigenAnnotator(AbstractAnnotator): def __init__(self, references: ReferenceFolder, configuration: DependenciesConfiguration, - tcell_predictor: TcellPrediction, self_similarity: SelfSimilarityCalculator, + self_similarity: SelfSimilarityCalculator, rank_mhci_threshold=neofox.RANK_MHCI_THRESHOLD_DEFAULT, rank_mhcii_threshold=neofox.RANK_MHCII_THRESHOLD_DEFAULT): """class to annotate neoantigens""" - super().__init__(references, configuration, tcell_predictor, self_similarity) + super().__init__(references, configuration, self_similarity) self.proteome_db = references.proteome_db self.available_alleles = references.get_available_alleles() self.rank_mhci_threshold = rank_mhci_threshold @@ -162,14 +161,6 @@ def get_annotated_neoantigen(self, neoantigen: Neoantigen, patient: Patient, wit amplitude=self.amplitude) ) - # T cell predictor - if netmhcpan: - neoantigen.neofox_annotations.annotations.extend( - self.tcell_predictor.get_annotations( - neoantigen=neoantigen, netmhcpan=netmhcpan - ) - ) - # self-similarity neoantigen.neofox_annotations.annotations.extend( self.self_similarity.get_annnotations( diff --git a/neofox/annotator/neoepitope_annotator.py b/neofox/annotator/neoepitope_annotator.py index 091c113e..f98dc452 100755 --- a/neofox/annotator/neoepitope_annotator.py +++ b/neofox/annotator/neoepitope_annotator.py @@ -31,7 +31,6 @@ from neofox.MHC_predictors.netmhcpan.combine_netmhcpan_pred_multiple_binders import BestAndMultipleBinder from neofox.model.factories import AnnotationFactory from neofox.model.mhc_parser import MhcParser -from neofox.published_features.Tcell_predictor.tcellpredictor_wrapper import TcellPrediction from neofox.published_features.self_similarity.self_similarity import SelfSimilarityCalculator from neofox.published_features.expression import Expression from neofox.model.neoantigen import Patient, Neoantigen, Annotations, PredictedEpitope @@ -45,10 +44,10 @@ class NeoepitopeAnnotator(AbstractAnnotator): def __init__(self, references: ReferenceFolder, configuration: DependenciesConfiguration, - tcell_predictor: TcellPrediction, self_similarity: SelfSimilarityCalculator): + self_similarity: SelfSimilarityCalculator): """class to annotate neoantigens""" - super().__init__(references, configuration, tcell_predictor, self_similarity) + super().__init__(references, configuration, self_similarity) self.proteome_db = references.proteome_db self.available_alleles = references.get_available_alleles() diff --git a/neofox/annotator/neoepitope_mhc_binding_annotator.py b/neofox/annotator/neoepitope_mhc_binding_annotator.py index e4a6d20e..c7a846f0 100644 --- a/neofox/annotator/neoepitope_mhc_binding_annotator.py +++ b/neofox/annotator/neoepitope_mhc_binding_annotator.py @@ -59,6 +59,7 @@ def get_mhc_binding_annotations(self, neoepitope: PredictedEpitope) -> Predicted epitope=annotated_neoepitope, paired_epitope=mixmhcpred_neoepitope, annotation_name=MixMHCpred.ANNOTATION_PREFIX) + if mixmhcpred_neoepitope_wt: annotated_neoepitope = AnnotationFactory.annotate_epitope( epitope=annotated_neoepitope, paired_epitope=mixmhcpred_neoepitope_wt, @@ -70,6 +71,7 @@ def get_mhc_binding_annotations(self, neoepitope: PredictedEpitope) -> Predicted epitope=annotated_neoepitope, paired_epitope=prime_neoepitope, annotation_name=Prime.ANNOTATION_PREFIX) + if prime_neoepitope_wt: annotated_neoepitope = AnnotationFactory.annotate_epitope( epitope=annotated_neoepitope, paired_epitope=prime_neoepitope_wt, @@ -98,6 +100,8 @@ def _run_netmhcpan(self, neoepitope: PredictedEpitope) -> PredictedEpitope: # runs NetMHCpan in peptide mode over the mutated and WT separately and merges it back in one # predicted epitope annotated_neoepitope = neoepitope + annotated_neoepitope.affinity_wild_type = None + annotated_neoepitope.rank_wild_type = None netmhcpan_allele = self.mhc_parser.get_netmhcpan_representation(neoepitope.allele_mhc_i) if netmhcpan_allele in self.available_alleles.get_available_mhc_i(): @@ -105,14 +109,17 @@ def _run_netmhcpan(self, neoepitope: PredictedEpitope) -> PredictedEpitope: sequence=neoepitope.mutated_peptide, alleles=netmhcpan_allele) annotated_neoepitope.affinity_mutated = mutated_epitope.affinity_mutated annotated_neoepitope.rank_mutated = mutated_epitope.rank_mutated - wt_epitope = self.netmhcpan.mhc_prediction_peptide( - sequence=neoepitope.wild_type_peptide, alleles=netmhcpan_allele) - annotated_neoepitope.affinity_wild_type = wt_epitope.affinity_mutated - annotated_neoepitope.rank_wild_type = wt_epitope.rank_mutated + if neoepitope.wild_type_peptide: + wt_epitope = self.netmhcpan.mhc_prediction_peptide( + sequence=neoepitope.wild_type_peptide, alleles=netmhcpan_allele) + annotated_neoepitope.affinity_wild_type = wt_epitope.affinity_mutated + annotated_neoepitope.rank_wild_type = wt_epitope.rank_mutated return annotated_neoepitope def _run_netmhc2pan(self, neoepitope: PredictedEpitope) -> PredictedEpitope: annotated_neoepitope = neoepitope + annotated_neoepitope.affinity_wild_type = None + annotated_neoepitope.rank_wild_type = None netmhc2pan_allele = self.mhc_parser.get_netmhc2pan_representation(neoepitope.isoform_mhc_i_i) if netmhc2pan_allele in self.available_alleles.get_available_mhc_ii(): @@ -121,29 +128,40 @@ def _run_netmhc2pan(self, neoepitope: PredictedEpitope) -> PredictedEpitope: mhc2_isoform=neoepitope.isoform_mhc_i_i) annotated_neoepitope.affinity_mutated = mutated_epitope.affinity_mutated annotated_neoepitope.rank_mutated = mutated_epitope.rank_mutated - wt_epitope = self.netmhc2pan.mhc2_prediction_peptide( - sequence=neoepitope.wild_type_peptide, - mhc2_isoform=neoepitope.isoform_mhc_i_i) - annotated_neoepitope.affinity_wild_type = wt_epitope.affinity_mutated - annotated_neoepitope.rank_wild_type = wt_epitope.rank_mutated + if neoepitope.wild_type_peptide: + wt_epitope = self.netmhc2pan.mhc2_prediction_peptide( + sequence=neoepitope.wild_type_peptide, + mhc2_isoform=neoepitope.isoform_mhc_i_i) + annotated_neoepitope.affinity_wild_type = wt_epitope.affinity_mutated + annotated_neoepitope.rank_wild_type = wt_epitope.rank_mutated return annotated_neoepitope def _run_mixmhcpred(self, neoepitope: PredictedEpitope) -> Tuple[PredictedEpitope, PredictedEpitope]: + + wt_epitope = None + mutated_epitope = self.mixmhcpred.run_peptide( peptide=neoepitope.mutated_peptide, allele=neoepitope.allele_mhc_i) - wt_epitope = self.mixmhcpred.run_peptide( - peptide=neoepitope.wild_type_peptide, allele=neoepitope.allele_mhc_i) + if neoepitope.wild_type_peptide: + wt_epitope = self.mixmhcpred.run_peptide( + peptide=neoepitope.wild_type_peptide, allele=neoepitope.allele_mhc_i) return mutated_epitope, wt_epitope def _run_prime(self, neoepitope: PredictedEpitope) -> Tuple[PredictedEpitope, PredictedEpitope]: + + wt_epitope = None mutated_epitope = self.prime.run_peptide(peptide=neoepitope.mutated_peptide, allele=neoepitope.allele_mhc_i) - wt_epitope = self.prime.run_peptide( - peptide=neoepitope.wild_type_peptide, allele=neoepitope.allele_mhc_i) + if neoepitope.wild_type_peptide: + wt_epitope = self.prime.run_peptide( + peptide=neoepitope.wild_type_peptide, allele=neoepitope.allele_mhc_i) return mutated_epitope, wt_epitope def _run_mixmhc2pred(self, neoepitope: PredictedEpitope) -> Tuple[PredictedEpitope, PredictedEpitope]: + wt_epitope = None mutated_epitope = self.mixmhc2pred.run_peptide( isoform=neoepitope.isoform_mhc_i_i, peptide=neoepitope.mutated_peptide) - wt_epitope = self.mixmhc2pred.run_peptide( - peptide=neoepitope.wild_type_peptide, isoform=neoepitope.isoform_mhc_i_i) + + if neoepitope.wild_type_peptide: + wt_epitope = self.mixmhc2pred.run_peptide( + peptide=neoepitope.wild_type_peptide, isoform=neoepitope.isoform_mhc_i_i) return mutated_epitope, wt_epitope diff --git a/neofox/helpers/blastp_runner.py b/neofox/helpers/blastp_runner.py index 58807832..06478d08 100755 --- a/neofox/helpers/blastp_runner.py +++ b/neofox/helpers/blastp_runner.py @@ -19,8 +19,8 @@ from math import exp, log import json import subprocess -from Bio import pairwise2 -from Bio.SubsMat import MatrixInfo as matlist +from Bio.Align import PairwiseAligner +from Bio.Align import substitution_matrices from neofox.helpers.runner import Runner from neofox.references.references import DependenciesConfiguration @@ -63,9 +63,9 @@ def calculate_similarity_database(self, peptide, a=26) -> float: if "-" not in query and "-" not in target: al = BlastpRunner.align(query, target) if al and len(al) > 0: - local_alignments.append(al[0]) + local_alignments.append(al) similarity_score = self.computeR(alignments=local_alignments, a=a) - except SystemError: + except (SystemError, ValueError): # NOTE: some rarer aminoacids substitutions may not be present in the BLOSUM matrix and thus cause this to # fail, an example is U>Y similarity_score = None @@ -112,12 +112,11 @@ def align(seq1, seq2): """ Smith-Waterman alignment with default parameters. """ - matrix = matlist.blosum62 - gap_open = -11 - gap_extend = -1 - aln = pairwise2.align.localds( - seq1.upper(), seq2.upper(), matrix, gap_open, gap_extend - ) + aligner = PairwiseAligner() + aligner.open_gap_score = -11 + aligner.extend_gap_score = -1 + aligner.substitution_matrix = substitution_matrices.load("BLOSUM62") + aln = aligner.align(seq1.upper(), seq2.upper()) return aln @staticmethod @@ -126,7 +125,7 @@ def computeR(alignments, a=26, k=4.87) -> float: Compute TCR-recognition probabilities for each neoantigen. """ # energies of all bound states of neoantigen i - bindingEnergies = [-k * (a - el[2]) for el in alignments] + bindingEnergies = [-k * (a - el.score) for el in alignments] # partition function, over all bound states and an unbound state lZ = BlastpRunner.logSum(bindingEnergies + [0]) lGb = BlastpRunner.logSum(bindingEnergies) diff --git a/neofox/helpers/epitope_helper.py b/neofox/helpers/epitope_helper.py index 33540faf..2f66ee3a 100755 --- a/neofox/helpers/epitope_helper.py +++ b/neofox/helpers/epitope_helper.py @@ -68,10 +68,14 @@ def number_of_mismatches(epitope_wild_type, epitope_mutation): This function calculates the number of mismatches between the wt and the mutated epitope """ # TODO: this is not efficient, it can be done with zip or using some library implementing Levenhstein distance - p1 = 0 - for aa_mut, aa_wt in zip(epitope_mutation, epitope_wild_type): - if aa_mut != aa_wt: - p1 += 1 + + p1 = None + if epitope_wild_type and epitope_mutation: + p1 = 0 + for aa_mut, aa_wt in zip(epitope_mutation, epitope_wild_type): + if aa_mut != aa_wt: + p1 += 1 + p1 = int(p1) return p1 @staticmethod diff --git a/neofox/model/validation.py b/neofox/model/validation.py index 79c60d2c..dd61e88d 100755 --- a/neofox/model/validation.py +++ b/neofox/model/validation.py @@ -20,7 +20,6 @@ import csv import os import pandas as pd -from Bio.Alphabet.IUPAC import ExtendedIUPACProtein from Bio.Data import IUPACData from neofox.exceptions import NeofoxDataValidationException from logzero import logger @@ -311,7 +310,7 @@ def _validate_aminoacid(aminoacid): aminoacid = IUPACData.protein_letters_3to1_extended.get(aminoacid) if len(aminoacid) == 1: assert ( - aminoacid in ExtendedIUPACProtein.letters + aminoacid in IUPACData.extended_protein_letters ), "Non existing aminoacid {}".format(aminoacid) else: assert False, "Invalid aminoacid {}".format(aminoacid) diff --git a/neofox/neofox.py b/neofox/neofox.py index ff996284..6843d44f 100755 --- a/neofox/neofox.py +++ b/neofox/neofox.py @@ -28,7 +28,6 @@ import neofox from neofox.expression_imputation.expression_imputation import ExpressionAnnotator from neofox.model.factories import NeoantigenFactory -from neofox.published_features.Tcell_predictor.tcellpredictor_wrapper import TcellPrediction from neofox.published_features.self_similarity.self_similarity import SelfSimilarityCalculator from neofox.published_features.expression import Expression from neofox.references.references import ReferenceFolder, DependenciesConfiguration, ORGANISM_HOMO_SAPIENS @@ -79,7 +78,6 @@ def __init__( self.configuration = ( configuration if configuration else DependenciesConfiguration() ) - self.tcell_predictor = TcellPrediction() self.self_similarity = SelfSimilarityCalculator() self.num_cpus = num_cpus @@ -194,9 +192,6 @@ def send_to_client(self, dask_client): futures = [] start = time.time() # NOTE: sets those heavy resources to be used by all workers in the cluster - future_tcell_predictor = dask_client.scatter( - self.tcell_predictor, broadcast=True - ) future_self_similarity = dask_client.scatter(self.self_similarity, broadcast=True) future_reference_folder = dask_client.scatter(self.reference_folder, broadcast=True) future_configuration = dask_client.scatter(self.configuration, broadcast=True) @@ -211,7 +206,6 @@ def send_to_client(self, dask_client): patient, future_reference_folder, future_configuration, - future_tcell_predictor, future_self_similarity, self.log_file_name, self.rank_mhci_threshold, @@ -235,7 +229,6 @@ def annotate_neoantigen( patient: Patient, reference_folder: ReferenceFolder, configuration: DependenciesConfiguration, - tcell_predictor: TcellPrediction, self_similarity: SelfSimilarityCalculator, log_file_name: str, rank_mhci_threshold = neofox.RANK_MHCI_THRESHOLD_DEFAULT, @@ -251,7 +244,6 @@ def annotate_neoantigen( annotated_neoantigen = NeoantigenAnnotator( reference_folder, configuration, - tcell_predictor=tcell_predictor, self_similarity=self_similarity, rank_mhci_threshold=rank_mhci_threshold, rank_mhcii_threshold=rank_mhcii_threshold diff --git a/neofox/neofox_epitope.py b/neofox/neofox_epitope.py index a0dd077c..5ec65de2 100755 --- a/neofox/neofox_epitope.py +++ b/neofox/neofox_epitope.py @@ -27,7 +27,6 @@ from neofox.annotator.neoepitope_annotator import NeoepitopeAnnotator from neofox.expression_imputation.expression_imputation import ExpressionAnnotator -from neofox.published_features.Tcell_predictor.tcellpredictor_wrapper import TcellPrediction from neofox.published_features.self_similarity.self_similarity import SelfSimilarityCalculator from neofox.references.references import ReferenceFolder, DependenciesConfiguration, ORGANISM_HOMO_SAPIENS from neofox.exceptions import NeofoxConfigurationException, NeofoxDataValidationException @@ -69,7 +68,6 @@ def __init__( self.configuration = ( configuration if configuration else DependenciesConfiguration() ) - self.tcell_predictor = TcellPrediction() self.self_similarity = SelfSimilarityCalculator() self.num_cpus = num_cpus @@ -136,9 +134,6 @@ def send_to_client(self, dask_client): futures = [] start = time.time() # NOTE: sets those heavy resources distributed to all workers in the cluster - future_tcell_predictor = dask_client.scatter( - self.tcell_predictor, broadcast=True - ) future_self_similarity = dask_client.scatter(self.self_similarity, broadcast=True) future_reference_folder = dask_client.scatter(self.reference_folder, broadcast=True) future_configuration = dask_client.scatter(self.configuration, broadcast=True) @@ -151,7 +146,6 @@ def send_to_client(self, dask_client): neoepitope, future_reference_folder, future_configuration, - future_tcell_predictor, future_self_similarity, self.log_file_name, self.verbose, @@ -166,7 +160,6 @@ def send_to_client(self, dask_client): ) # close distributed resources - del future_tcell_predictor del future_self_similarity del future_reference_folder del future_configuration @@ -178,7 +171,6 @@ def annotate_neoepitope( neoepitope: PredictedEpitope, reference_folder: ReferenceFolder, configuration: DependenciesConfiguration, - tcell_predictor: TcellPrediction, self_similarity: SelfSimilarityCalculator, log_file_name: str, verbose = False @@ -191,7 +183,6 @@ def annotate_neoepitope( annotated_neoantigen = NeoepitopeAnnotator( reference_folder, configuration, - tcell_predictor=tcell_predictor, self_similarity=self_similarity, ).get_annotated_neoepitope(neoepitope) except Exception as e: diff --git a/neofox/published_features/Tcell_predictor/Classifier.pickle b/neofox/published_features/Tcell_predictor/Classifier.pickle deleted file mode 100755 index 13d21e1f49644146b22dd4d3f5ebf242bb4b23aa..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 12320161 zcmdqKc|eub_CLIx$CMnw@ra0sq=-nU=-z-cXozNN4xmD4PB~OId2WMgUv62_Ky}T$ zX2b1vEz8VHaw=0(%6USj%+SorIW^1odG=asKhJp%xKH-};r*RIeBA7LTI;jdT6=AG 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See the -# GNU General Public License for more details. -# -# You should have received a copy of the GNU General Public License -# along with this program. 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zlsv$VggpCLY$H+)B{cwco87*X)7_rgSMlV@@_oDT&-t~?SgFpoCyhk0z#DQmwizYh| z7sbG)Z+n({= zwIbngy67r>YX{QiGXGoZ)gc6^Rb2ZxvolaIbaLxkor}j7>$X-{=yjK{lhSUAFGKJt zkIuBEFj`!)6wqVueOKixcfT4JWPY207U?sN7+lYFG#U&Rt5Cesht8T^9`)f2hatBN zC{Jv&^iRmnBN@1*KNyf(%ciX>Ym0%&qhxL`Kan(Vv#KlzI$oXSimj3AoF! zQ)HS7BlMK*s4(mgcymr)Dy%N|=)2o$z^obMkv5cOX6L4n#GYv?%=P-yIHA*Lif9<} zq)l5U=4TIN8MQEG2zKUy5}X{BT8_61!|B0w*=G>42-;*vht*#g5BKHx}Dqx0FG3Mf*_ zNdB$Y2Dq@y;x>|LGtS74abnc#=Qf_d^fdkCc0|c0n`.# -import pickle - -import numpy as np -import scipy.io as sio -import os - -from Bio.Data import IUPACData - -from neofox.helpers.epitope_helper import EpitopeHelper -from neofox.model.neoantigen import PredictedEpitope - -from functools import lru_cache - -MAT = "SIRdata.mat" -GENES_EXPRESSION_PICKLE = "genes-expression.pickle" -ACIDS_FEATURES_PICKLE = "amino-acids-features.pickle" - - -class Preprocessor(object): - def __init__(self): - self.load_data = sio.loadmat( - os.path.join(os.path.abspath(os.path.dirname(__file__)), MAT) - ) - with open( - os.path.join( - os.path.abspath(os.path.dirname(__file__)), GENES_EXPRESSION_PICKLE - ), - "rb", - ) as handle: - self.dict_expression = pickle.load(handle) - with open( - os.path.join( - os.path.abspath(os.path.dirname(__file__)), ACIDS_FEATURES_PICKLE - ), - "rb", - ) as handle: - self.dict_data = pickle.load(handle) - - @staticmethod - def seq2bin(seq): - aa = list(IUPACData.protein_letters_3to1.values()) - dict_aa = dict((i, j) for j, i in enumerate(aa)) - arr = np.zeros((1, 9 * 20)) - for ii, letter in enumerate(seq): - arr[0, ii * 20 + dict_aa.get(letter)] = 1 - return arr - - @staticmethod - def get_hydrophbicity(x, dict_): - pair_letters = [c for c in x if c.isupper()] - res = np.abs(dict_[pair_letters[0]] - dict_[pair_letters[1]]) - return res - - @staticmethod - def get_size(x, dict_): - pair_letters = [c for c in x if c.isupper()] - res = np.abs(dict_[pair_letters[0]] - dict_[pair_letters[1]]) - return res - - @staticmethod - def get_charge_change(x, dict_): - pair_letters = [c for c in x if c.isupper()] - if dict_[pair_letters[0]] == dict_[pair_letters[1]]: - return 0 - else: - return 1 - - @staticmethod - def get_charge_abs(x, dict_): - pair_letters = [c for c in x if c.isupper()] - if dict_[pair_letters[0]] == dict_[pair_letters[1]]: - return 0 - else: - return 1 - - @staticmethod - def get_polar(x, dict_): - pair_letters = [c for c in x if c.isupper()] - res = np.abs(dict_[pair_letters[0]] - dict_[pair_letters[1]]) - return res - - @staticmethod - def get_absolute(x, dict_): - pair_letters = [c for c in x if c.isupper()] - res = np.abs(dict_[pair_letters[0]] - dict_[pair_letters[1]]) - return res - - @staticmethod - def get_diffetenet(x, dict_): - pair_letters = [c for c in x if c.isupper()] - if dict_[pair_letters[0]] == dict_[pair_letters[1]]: - return 0 - else: - return 1 - - def get_gene_expression(self, gene): - res = self.dict_expression.get(gene, 0.0) - return res - - def get_properties(self, amino_substitution): - return np.asarray( - [ - self.get_diffetenet(amino_substitution, self.dict_data["Charge"]), - self.get_absolute(amino_substitution, self.dict_data["Size"]), - self.get_absolute(amino_substitution, self.dict_data["Hydro"]), - self.get_absolute(amino_substitution, self.dict_data["Charge"]), - self.get_diffetenet(amino_substitution, self.dict_data["Polar"]), - ] - ) - - def main(self, gene: str, epitope: PredictedEpitope): - - seq_arr = self.seq2bin(epitope.mutated_peptide) - # tap score - tap_mat = self.load_data.get("tap") - tap_score = tap_mat.dot(seq_arr.T).ravel() - # cleavge score - clv_mat = self.load_data.get("clv") - clv_mat = clv_mat[0, 20:200] - clv_score = clv_mat.dot(seq_arr.T).ravel() - - position_of_mutation = EpitopeHelper.position_of_mutation_epitope(epitope=epitope) - wild_type_aminoacid = epitope.wild_type_peptide[position_of_mutation - 1] # it is 1-based - mutated_aminoacid = epitope.mutated_peptide[position_of_mutation - 1] - features_aa = self.get_properties(wild_type_aminoacid + mutated_aminoacid) - # expresion - expression_value = self.get_gene_expression(gene) - - result = np.hstack((expression_value, features_aa, clv_score, tap_score)) - - mat_features = np.asarray([result]) - - return mat_features diff --git a/neofox/published_features/Tcell_predictor/tcellpredictor_wrapper.py b/neofox/published_features/Tcell_predictor/tcellpredictor_wrapper.py deleted file mode 100755 index f380ce84..00000000 --- a/neofox/published_features/Tcell_predictor/tcellpredictor_wrapper.py +++ /dev/null @@ -1,105 +0,0 @@ -#!/usr/bin/env python -# -# Copyright (c) 2020-2030 Translational Oncology at the Medical Center of the Johannes Gutenberg-University Mainz gGmbH. -# -# This file is part of Neofox -# (see https://github.com/tron-bioinformatics/neofox). -# -# This program is free software: you can redistribute it and/or modify -# it under the terms of the GNU General Public License as published by -# the Free Software Foundation, either version 3 of the License, or -# (at your option) any later version. -# -# This program is distributed in the hope that it will be useful, -# but WITHOUT ANY WARRANTY; without even the implied warranty of -# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the -# GNU General Public License for more details. -# -# You should have received a copy of the GNU General Public License -# along with this program. If not, see .# -import os -import pickle -import warnings -from typing import List -from neofox.model.validation import ModelValidator -from neofox.model.neoantigen import Annotation, Neoantigen, PredictedEpitope -from neofox.model.factories import AnnotationFactory -from neofox.published_features.Tcell_predictor.preprocess import Preprocessor -from neofox.MHC_predictors.netmhcpan.combine_netmhcpan_pred_multiple_binders import ( - BestAndMultipleBinder, -) - -CLASSIFIER_PICKLE = "Classifier.pickle" - - -class TcellPrediction: - - def __init__(self): - # UserWarning: Trying to unpickle estimator DecisionTreeClassifier from version 0.19.0 when using version - # 0.20.3. This might lead to breaking code or invalid results. Use at your own risk. - with warnings.catch_warnings(): - warnings.simplefilter("ignore") - with open( - os.path.join( - os.path.abspath(os.path.dirname(__file__)), CLASSIFIER_PICKLE - ), - 'rb' - ) as f: - # this sets the n_jobs parameter otherwise inherited from the pickle file - self.classifier = pickle.load(f).set_params(n_jobs=1) - - self.preprocessor = Preprocessor() - - def _wrapper_tcellpredictor(self, gene, epitope: PredictedEpitope): - """ - wrapper function to determine - """ - result = None - has_gene = gene is not None and gene.strip() != "" - if has_gene and len(epitope.mutated_peptide) == 9: - mat = self.preprocessor.main(gene, epitope=epitope) - scores = self.classifier.predict_proba(mat) - result = "indefinable_by_TcellPredictor" - if ( - scores is not None - and len(scores) > 0 - and scores[-1] is not None - and len(scores[-1]) > 0 - ): - # it returns the last number from the latest entry in the list - result = str(scores[-1][-1]) - return result - - def calculate_tcell_predictor_score( - self, gene: str, epitope: PredictedEpitope - ): - """returns Tcell_predictor score given mps in dictionary format""" - tcell_predictor_score = None - if not ModelValidator.has_peptide_rare_amino_acids(epitope.mutated_peptide): - tcell_predictor_score = self._wrapper_tcellpredictor(gene=gene, epitope=epitope) - return tcell_predictor_score - - def get_annotations( - self, neoantigen: Neoantigen, netmhcpan: BestAndMultipleBinder - ) -> List[Annotation]: - # TODO: this is difficult to extend to more complex mutations (eg: MNVs, indels) as only considers first mutated - # position - tcell_predictor_score = None - if neoantigen.wild_type_xmer and netmhcpan.best_ninemer_epitope_by_affinity.mutated_peptide: - tcell_predictor_score = self.calculate_tcell_predictor_score( - gene=neoantigen.gene, - epitope=netmhcpan.best_ninemer_epitope_by_affinity) - annotations = [ - AnnotationFactory.build_annotation( - value=tcell_predictor_score, - name="Tcell_predictor", - ) - ] - return annotations - - def get_annotations_epitope_mhci(self, epitope: PredictedEpitope, gene: str) -> List[Annotation]: - return [ - AnnotationFactory.build_annotation( - value=self.calculate_tcell_predictor_score(epitope=epitope, gene=gene), - name='Tcell_predictor') - ] diff --git a/neofox/published_features/hex/pyhex.py b/neofox/published_features/hex/pyhex.py index 9db1e6d6..7d6576fe 100644 --- a/neofox/published_features/hex/pyhex.py +++ b/neofox/published_features/hex/pyhex.py @@ -2,7 +2,7 @@ from Bio import SeqIO from Bio.Align import substitution_matrices -from Bio.Alphabet.IUPAC import ExtendedIUPACProtein +from Bio.Data.IUPACData import protein_letters class PyHex: @@ -19,7 +19,7 @@ def _read_fasta(fasta_file): with open(fasta_file, "r") as handle: for record in SeqIO.parse(handle, "fasta"): # include only records that do not contain non-standard amino acids - if not any([aa not in ExtendedIUPACProtein.letters for aa in record.seq]): + if not any([aa not in protein_letters for aa in record.seq]): sequences.append(record) return sequences diff --git a/neofox/published_features/priority_score.py b/neofox/published_features/priority_score.py index e849e174..9042955f 100755 --- a/neofox/published_features/priority_score.py +++ b/neofox/published_features/priority_score.py @@ -160,50 +160,67 @@ def get_annotations( def get_annotations_epitope_mhci(self, epitope: PredictedEpitope, vaf_tumor, transcript_exp, vaf_rna, gene_exp) -> \ List[Annotation]: - return [ - AnnotationFactory.build_annotation( - value=self.calc_priority_score( + + priority_score_dna = None + priority_score_rna = None + priority_score_imputed_dna = None + priority_score_imputed_rna = None + + if epitope.mutated_peptide and epitope.rank_wild_type: + priority_score_dna = self.calc_priority_score( vaf=vaf_tumor, transcript_gene_expr=transcript_exp, - no_mismatch=int(EpitopeHelper.get_annotation_by_name( - epitope.neofox_annotations.annotations, name='number_of_mismatches')), + no_mismatch=EpitopeHelper.get_annotation_by_name( + epitope.neofox_annotations.annotations, name='number_of_mismatches'), score_mut=epitope.rank_mutated, score_wt=epitope.rank_wild_type, mut_not_in_prot=bool(EpitopeHelper.get_annotation_by_name( - epitope.neofox_annotations.annotations, name='mutation_not_found_in_proteome'))), - name='Priority_score_fromDNA'), - AnnotationFactory.build_annotation( - value=self.calc_priority_score( + epitope.neofox_annotations.annotations, name='mutation_not_found_in_proteome'))) + + if epitope.mutated_peptide and epitope.rank_wild_type: + priority_score_imputed_dna = priority_score_rna = self.calc_priority_score( vaf=vaf_tumor, transcript_gene_expr=gene_exp, - no_mismatch=int(EpitopeHelper.get_annotation_by_name( - epitope.neofox_annotations.annotations, name='number_of_mismatches')), + no_mismatch=EpitopeHelper.get_annotation_by_name( + epitope.neofox_annotations.annotations, name='number_of_mismatches'), score_mut=epitope.rank_mutated, score_wt=epitope.rank_wild_type, mut_not_in_prot=bool(EpitopeHelper.get_annotation_by_name( - epitope.neofox_annotations.annotations, name='mutation_not_found_in_proteome'))), - name='Priority_score_imputed_fromDNA'), - AnnotationFactory.build_annotation( - value=self.calc_priority_score( + epitope.neofox_annotations.annotations, name='mutation_not_found_in_proteome'))) + + if epitope.mutated_peptide and epitope.rank_wild_type: + priority_score_rna = self.calc_priority_score( vaf=vaf_rna, transcript_gene_expr=transcript_exp, - no_mismatch=int(EpitopeHelper.get_annotation_by_name( - epitope.neofox_annotations.annotations, name='number_of_mismatches')), + no_mismatch=EpitopeHelper.get_annotation_by_name( + epitope.neofox_annotations.annotations, name='number_of_mismatches'), score_mut=epitope.rank_mutated, score_wt=epitope.rank_wild_type, mut_not_in_prot=bool(EpitopeHelper.get_annotation_by_name( - epitope.neofox_annotations.annotations, name='mutation_not_found_in_proteome'))), - name='Priority_score_fromRNA'), - AnnotationFactory.build_annotation( - value=self.calc_priority_score( + epitope.neofox_annotations.annotations, name='mutation_not_found_in_proteome'))) + + if epitope.mutated_peptide and epitope.rank_wild_type: + priority_score_imputed_rna = self.calc_priority_score( vaf=vaf_rna, transcript_gene_expr=gene_exp, - no_mismatch=int(EpitopeHelper.get_annotation_by_name( - epitope.neofox_annotations.annotations, name='number_of_mismatches')), + no_mismatch=EpitopeHelper.get_annotation_by_name( + epitope.neofox_annotations.annotations, name='number_of_mismatches'), score_mut=epitope.rank_mutated, score_wt=epitope.rank_wild_type, mut_not_in_prot=bool(EpitopeHelper.get_annotation_by_name( - epitope.neofox_annotations.annotations, name='mutation_not_found_in_proteome'))), - name='Priority_score_imputed_fromRNA'), + epitope.neofox_annotations.annotations, name='mutation_not_found_in_proteome'))) + return [ + AnnotationFactory.build_annotation( + value=priority_score_dna, + name='Priority_score_fromDNA'), + AnnotationFactory.build_annotation( + value=priority_score_imputed_dna, + name='Priority_score_imputed_fromDNA'), + AnnotationFactory.build_annotation( + value=priority_score_rna, + name='Priority_score_fromRNA'), + AnnotationFactory.build_annotation( + value=priority_score_imputed_rna, + name='Priority_score_imputed_fromRNA'), ] diff --git a/neofox/published_features/self_similarity/self_similarity.py b/neofox/published_features/self_similarity/self_similarity.py index 2358a508..b8277511 100755 --- a/neofox/published_features/self_similarity/self_similarity.py +++ b/neofox/published_features/self_similarity/self_similarity.py @@ -101,11 +101,11 @@ def get_self_similarity(self, mutated_peptide, wt_peptide): """ self_similarity = None if not ModelValidator.has_peptide_rare_amino_acids(mutated_peptide) and \ - not ModelValidator.has_peptide_rare_amino_acids(wt_peptide): - try: - self_similarity = str(self.compute_k_hat_3(mutated_peptide, wt_peptide)) - except ZeroDivisionError: - pass + not ModelValidator.has_peptide_rare_amino_acids(wt_peptide): + try: + self_similarity = str(self.compute_k_hat_3(mutated_peptide, wt_peptide)) + except ZeroDivisionError: + pass return self_similarity def is_improved_binder(self, score_mutation, score_wild_type) -> bool: @@ -176,24 +176,34 @@ def get_annnotations( return annotations def get_annotations_epitope_mhcii(self, epitope: PredictedEpitope) -> List[Annotation]: + + self_similarity_mhcii = None + if epitope.mutated_peptide and epitope.wild_type_peptide: + self_similarity_mhcii = self.get_self_similarity( + mutated_peptide=epitope.mutated_peptide, wt_peptide=epitope.wild_type_peptide) + return [ AnnotationFactory.build_annotation( - value=self.get_self_similarity( - mutated_peptide=epitope.mutated_peptide, wt_peptide=epitope.wild_type_peptide), + value=self_similarity_mhcii, name='Selfsimilarity') ] def get_annotations_epitope_mhci(self, epitope: PredictedEpitope) -> List[Annotation]: - is_improved_binder = self.is_improved_binder( - score_mutation=epitope.rank_mutated, score_wild_type=epitope.rank_wild_type) - self_similarity = self.get_self_similarity( - mutated_peptide=epitope.mutated_peptide, wt_peptide=epitope.wild_type_peptide) + + is_improved_binder = None + self_similarity_mhci = None + + if epitope.rank_mutated and epitope.rank_wild_type: + is_improved_binder = self.is_improved_binder( + score_mutation=epitope.rank_mutated, score_wild_type=epitope.rank_wild_type) + self_similarity_mhci = self.get_self_similarity( + mutated_peptide=epitope.mutated_peptide, wt_peptide=epitope.wild_type_peptide) return [ AnnotationFactory.build_annotation(value=is_improved_binder, name='Improved_Binder_MHCI'), - AnnotationFactory.build_annotation(value=self_similarity, name='Selfsimilarity'), + AnnotationFactory.build_annotation(value=self_similarity_mhci, name='Selfsimilarity'), AnnotationFactory.build_annotation( value=self.self_similarity_of_conserved_binder_only( - similarity=self_similarity, is_improved_binder=is_improved_binder), + similarity=self_similarity_mhci, is_improved_binder=is_improved_binder), name='Selfsimilarity_conserved_binder') ] \ No newline at end of file diff --git a/neofox/references/installer.py b/neofox/references/installer.py index 7ae2ebbc..d04c1153 100644 --- a/neofox/references/installer.py +++ b/neofox/references/installer.py @@ -5,8 +5,7 @@ from shutil import copyfile import pandas as pd from Bio import SeqIO -from Bio.Alphabet import _verify_alphabet, IUPAC -from Bio.Seq import Seq +from Bio.Data.IUPACData import protein_letters from datetime import datetime import hashlib import xmltodict @@ -407,7 +406,7 @@ def build_fasta(self, organism, process_type, output_file): filtered_iedb.loc[:, "seq"] = filtered_iedb.loc[:, "Epitope:Name"].transform( lambda x: x.strip()) filtered_iedb.loc[:, "valid_peptide"] = filtered_iedb.loc[:, "seq"].transform( - lambda x: _verify_alphabet(Seq(x, IUPAC.protein))) + lambda x: all(aa in protein_letters for aa in x)) filtered_iedb = filtered_iedb[filtered_iedb.valid_peptide] # build fasta header: 449|FL-160-2 protein - Trypanosoma cruzi|JH0823|Trypanosoma cruzi|5693 diff --git a/neofox/tests/integration_tests/integration_test_tools.py b/neofox/tests/integration_tests/integration_test_tools.py index 53c60695..910c4a98 100755 --- a/neofox/tests/integration_tests/integration_test_tools.py +++ b/neofox/tests/integration_tests/integration_test_tools.py @@ -21,7 +21,7 @@ from unittest import TestCase import dotenv -from Bio.Alphabet.IUPAC import IUPACData +from Bio.Data import IUPACData from neofox.helpers.epitope_helper import EpitopeHelper from neofox.model.factories import MhcFactory @@ -204,7 +204,6 @@ def assert_neoepitope_mhci(self, original_neoepitope: PredictedEpitope, annotate # others to comes self.assert_annotation(annotated_neoepitope, annotation_name="Priority_score_fromDNA") - self.assert_annotation(annotated_neoepitope, annotation_name="Tcell_predictor") def assert_neoepitope_mhcii(self, original_neoepitope: PredictedEpitope, annotated_neoepitope: PredictedEpitope): self.assertIsNotNone(annotated_neoepitope) diff --git a/neofox/tests/integration_tests/test_dissimilarity.py b/neofox/tests/integration_tests/test_dissimilarity.py index 118a22df..f1694402 100755 --- a/neofox/tests/integration_tests/test_dissimilarity.py +++ b/neofox/tests/integration_tests/test_dissimilarity.py @@ -47,7 +47,8 @@ def test_similar_sequences(self): PredictedEpitope(mutated_peptide="DDDDDD", affinity_mutated=600)) self.assertTrue(result < 0.000001) - def test_missing_aminoacid_change(self): + def test_blosum_nonexisting_aminoacid_change(self): + # Amino acid U does not exist in BLOSUM62 matrix result = DissimilarityCalculator(proteome_blastp_runner=self.proteome_blastp_runner).calculate_dissimilarity( PredictedEpitope(mutated_peptide="DDUDDD", affinity_mutated=600)) self.assertIsNone(result) diff --git a/neofox/tests/integration_tests/test_neoantigen_annotator.py b/neofox/tests/integration_tests/test_neoantigen_annotator.py index 6df925e2..071c35eb 100644 --- a/neofox/tests/integration_tests/test_neoantigen_annotator.py +++ b/neofox/tests/integration_tests/test_neoantigen_annotator.py @@ -3,7 +3,6 @@ from neofox.annotator.neoantigen_annotator import NeoantigenAnnotator from neofox.model.factories import MhcFactory, NeoantigenFactory from neofox.model.neoantigen import PredictedEpitope, MhcAllele, Neoantigen, Mhc2Isoform, Patient -from neofox.published_features.Tcell_predictor.tcellpredictor_wrapper import TcellPrediction from neofox.published_features.self_similarity.self_similarity import SelfSimilarityCalculator from neofox.tests.integration_tests import integration_test_tools from neofox.tests.integration_tests.integration_test_tools import get_hla_one_test, get_hla_two_test @@ -16,7 +15,6 @@ def setUp(self) -> None: self.annotator = NeoantigenAnnotator( references=self.references, configuration=self.configuration, - tcell_predictor=TcellPrediction(), self_similarity=SelfSimilarityCalculator() ) self.patient = Patient( @@ -73,6 +71,20 @@ def test_neoantigen_annotation_without_wild_type_and_with_all_epitopes(self): self.assertIsNone(annotated_neoantigen.wild_type_xmer) self._assert_epitopes(annotated_neoantigen, with_all_epitopes=True) + def test_neoantigen_annotation_without_predicted_wild_type_and_with_all_epitopes(self): + neoantigen = NeoantigenFactory.build_neoantigen( + mutated_xmer="ALSIEFQEPVPASMMKVVQVYADTYT", + patient_identifier="123", + gene="BRCA2" + ) + annotated_neoantigen = self.annotator.get_annotated_neoantigen( + neoantigen=neoantigen, patient=self.patient, with_all_neoepitopes=True) + self._assert_neoantigen(annotated_neoantigen, neoantigen) + # wild type xmer is still empty! + self.assertIsNone(annotated_neoantigen.wild_type_xmer) + self._assert_epitopes(annotated_neoantigen, with_all_epitopes=True) + + def test_neoantigen_annotation_with_vaf_and_without_tx_expression(self): neoantigen = NeoantigenFactory.build_neoantigen( wild_type_xmer="DEVLGEPSQDILVIDQTRLEATISPET", diff --git a/neofox/tests/integration_tests/test_neoepitope_annotator.py b/neofox/tests/integration_tests/test_neoepitope_annotator.py index 01f0c10e..da11af3d 100644 --- a/neofox/tests/integration_tests/test_neoepitope_annotator.py +++ b/neofox/tests/integration_tests/test_neoepitope_annotator.py @@ -2,7 +2,6 @@ from neofox.helpers.epitope_helper import EpitopeHelper from neofox.model.factories import MhcFactory from neofox.model.neoantigen import PredictedEpitope, MhcAllele, Mhc2Isoform, Patient -from neofox.published_features.Tcell_predictor.tcellpredictor_wrapper import TcellPrediction from neofox.published_features.self_similarity.self_similarity import SelfSimilarityCalculator from neofox.tests.integration_tests.integration_test_tools import get_hla_one_test, get_hla_two_test, \ BaseIntegrationTest @@ -15,7 +14,6 @@ def setUp(self) -> None: self.annotator = NeoepitopeAnnotator( references=self.references, configuration=self.configuration, - tcell_predictor=TcellPrediction(), self_similarity=SelfSimilarityCalculator() ) self.patient = Patient( @@ -45,6 +43,16 @@ def test_neoepitope_mhci_without_wild_type(self): annotated_neoepitope = self.annotator.get_annotated_neoepitope(neoepitope=neoepitope) self.assert_neoepitope_mhci(original_neoepitope=neoepitope, annotated_neoepitope=annotated_neoepitope) + def test_neoepitope_mhci_without_predicted_wild_type(self): + + neoepitope = PredictedEpitope( + mutated_peptide="ASMMKVVQV", + allele_mhc_i=self._get_test_mhci_allele('HLA-A*01:01') + ) + + annotated_neoepitope = self.annotator.get_annotated_neoepitope(neoepitope=neoepitope) + self.assert_neoepitope_mhci(original_neoepitope=neoepitope, annotated_neoepitope=annotated_neoepitope) + def test_neoepitope_mhci_9mer_with_frequencies_and_gene(self): """ this checks fields that are only annotated when expression, vaf and/or gene are provided @@ -62,9 +70,8 @@ def test_neoepitope_mhci_9mer_with_frequencies_and_gene(self): annotated_neoepitope = self.annotator.get_annotated_neoepitope(neoepitope=neoepitope) self.assert_neoepitope_mhci(original_neoepitope=neoepitope, annotated_neoepitope=annotated_neoepitope) self.assert_float_annotation(annotated_neoepitope, annotation_name="Priority_score_fromDNA") - self.assert_float_annotation(annotated_neoepitope, annotation_name="Tcell_predictor") - def test_neoepitope_mhci_10mer_no_tcell_predictor(self): + def test_neoepitope_mhci_10mer(self): """ this checks fields that are only annotated when expression, vaf and/or gene are provided """ @@ -77,9 +84,6 @@ def test_neoepitope_mhci_10mer_no_tcell_predictor(self): annotated_neoepitope = self.annotator.get_annotated_neoepitope(neoepitope=neoepitope) self.assert_neoepitope_mhci(original_neoepitope=neoepitope, annotated_neoepitope=annotated_neoepitope) - annotation_value = EpitopeHelper.get_annotation_by_name( - annotated_neoepitope.neofox_annotations.annotations, "Tcell_predictor") - self.assertEqual(annotation_value, "NA") def test_neoepitope_mhci_without_dna_vaf(self): """ diff --git a/neofox/tests/integration_tests/test_neofox.py b/neofox/tests/integration_tests/test_neofox.py index 2973ad0b..11822f5a 100755 --- a/neofox/tests/integration_tests/test_neofox.py +++ b/neofox/tests/integration_tests/test_neofox.py @@ -204,7 +204,7 @@ def test_neofox_model_input(self): ).get_annotations() self.assertEqual(5, len(annotations)) self.assertIsInstance(annotations[0], Neoantigen) - self.assertEqual(len(annotations[0].neofox_annotations.annotations), 104) + self.assertEqual(len(annotations[0].neofox_annotations.annotations), 103) def test_neofox_without_mixmhcpreds(self): """ @@ -328,7 +328,7 @@ def test_neofox_without_mhc2(self): ).get_annotations() self.assertEqual(5, len(annotations)) self.assertIsInstance(annotations[0], Neoantigen) - self.assertEqual(len(annotations[0].neofox_annotations.annotations), 79) + self.assertEqual(len(annotations[0].neofox_annotations.annotations), 78) def test_neofox_without_mhc1(self): neoantigens, patients = self._get_test_data() @@ -724,7 +724,7 @@ def _check_single_value(self, s1, s2): return is_equal def _is_float(self, s1): - return isinstance(s1, float) or isinstance(s1, np.float) + return isinstance(s1, float) def _check_columns(self, new_df, previous_df): shared_columns = set(previous_df.columns).intersection(set(new_df.columns)) diff --git a/neofox/tests/integration_tests/test_prime.py b/neofox/tests/integration_tests/test_prime.py index fd105575..6588a845 100755 --- a/neofox/tests/integration_tests/test_prime.py +++ b/neofox/tests/integration_tests/test_prime.py @@ -44,10 +44,10 @@ def test_prime_epitope(self): self.prime.run(neoantigen=neoantigen, mhc=self.test_mhc_one, uniprot=self.uniprot) best_result = EpitopeHelper.select_best_by_affinity( predictions=self.prime.results, maximum=True) - self.assertEquals("LVTDQTRL", best_result.mutated_peptide) + self.assertEqual("LVTDQTRL", best_result.mutated_peptide) self.assertAlmostEqual(0.001858 , best_result.affinity_mutated, delta=0.00001) - self.assertEquals(18.992, best_result.rank_mutated) - self.assertEquals("HLA-C*05:01", best_result.allele_mhc_i.name) + self.assertEqual(18.992, best_result.rank_mutated) + self.assertEqual("HLA-C*05:01", best_result.allele_mhc_i.name) def test_prime_too_small_epitope(self): neoantigen = get_neoantigen(mutated_xmer="NLVP", wild_type_xmer="NLNP") diff --git a/neofox/tests/resources/test_data_model_realistic.txt b/neofox/tests/resources/test_data_model_realistic.txt index d854754b..fef900d0 100755 --- a/neofox/tests/resources/test_data_model_realistic.txt +++ b/neofox/tests/resources/test_data_model_realistic.txt @@ -1,11 +1,11 @@ -gene wildTypeXmer mutatedXmer patientIdentifier external_annotation_1 external_annotation_2 rnaExpression rnaVaf dnaVaf -HOXD10 RKKRCPYTKHQTLELERVLVQYVPHPRAPPRDQ Ptx blah1 blah2 0.250592319 0 0.160818713 -VCAN DEVLGEPSQDILVIDQTRLEATISPET DEVLGEPSQDILVTDQTRLEATISPET Ptx blah1 blah2 0.519506894 0.857142857 0.294573643 -DCST2 RTNLLAALHRSVRRRAADQGHRSAFLV RTNLLAALHRSVRWRAADQGHRSAFLV Ptx blah1 blah2 0.28245175 -1 0.108823529 -EXOSC2 SLKYGKLGQGVLVQVSPPW Ptx blah1 blah2 7.942770402 0.064 0.378881988 -NRAS MTEYKLVVVGAGGVGKSALTIQLIQ MTEYKLVVVGACGVGKSALTIQLIQ Ptx blah1 blah2 42.85666275 0.964426877 0.853658537 -CEP350 QTDSSSSDMQACSQDKAKISLGSSIDS QTDSSSSDMQACSKDKAKISLGSSIDS Ptx blah1 blah2 6.765113758 0.205128205 0.164754953 -CXorf26 YNKAVYISVQDKEGEKGVNNGGEKRAD YNKAVYISVQDKEEEKGVNNGGEKRAD Ptx blah1 blah2 15.38792717 0.534883721 0.46 -IGSF9B ASTHLTVIGTSPHAPGSVRVQVSMTTA ASTHLTVIGTSPHVPGSVRVQVSMTTA Ptx blah1 blah2 0.071129512 -1 0.422222222 -HEATR5A TRRDEKSHPFTNPRWATRVFAAECVCR TRRDEKSHPFTNPQWATRVFAAECVCR Ptx blah1 blah2 NA 0.947368421 0.799065421 -CHRDL2 ARPDMFCLFHGKRYSPGESWHPYLEPQ ARPDMFCLFHGKRHFPGESWHPYLEPQ Ptx blah1 blah2 NA 0 0.231884058 +gene wildTypeXmer mutatedXmer patientIdentifier external_annotation_1 external_annotation_2 rnaExpression rnaVaf dnaVaf +HOXD10 RKKRCPYTKHQTLELERVLVQYVPHPRAPPRDQ Ptx blah1 blah2 0.250592319 0 0.160818713 +VCAN DEVLGEPSQDILVIDQTRLEATISPET DEVLGEPSQDILVTDQTRLEATISPET Ptx blah1 blah2 0.519506894 0.857142857 0.294573643 +DCST2 RTNLLAALHRSVRRRAADQGHRSAFLV RTNLLAALHRSVRWRAADQGHRSAFLV Ptx blah1 blah2 0.28245175 -1 0.108823529 +EXOSC2 ALSIEFQEPVPASMMKVVQVYAVTQM Ptx blah1 blah2 7.942770402 0.064 0.378881988 +NRAS MTEYKLVVVGAGGVGKSALTIQLIQ MTEYKLVVVGACGVGKSALTIQLIQ Ptx blah1 blah2 42.85666275 0.964426877 0.853658537 +CEP350 QTDSSSSDMQACSQDKAKISLGSSIDS QTDSSSSDMQACSKDKAKISLGSSIDS Ptx blah1 blah2 6.765113758 0.205128205 0.164754953 +CXorf26 YNKAVYISVQDKEGEKGVNNGGEKRAD YNKAVYISVQDKEEEKGVNNGGEKRAD Ptx blah1 blah2 15.38792717 0.534883721 0.46 +IGSF9B ASTHLTVIGTSPHAPGSVRVQVSMTTA ASTHLTVIGTSPHVPGSVRVQVSMTTA Ptx blah1 blah2 0.071129512 -1 0.422222222 +HEATR5A TRRDEKSHPFTNPRWATRVFAAECVCR TRRDEKSHPFTNPQWATRVFAAECVCR Ptx blah1 blah2 NA 0.947368421 0.799065421 +CHRDL2 ARPDMFCLFHGKRYSPGESWHPYLEPQ ARPDMFCLFHGKRHFPGESWHPYLEPQ Ptx blah1 blah2 NA 0 0.231884058 diff --git a/neofox/tests/synthetic_data/factories.py b/neofox/tests/synthetic_data/factories.py index 0af11d2e..c482a6bd 100755 --- a/neofox/tests/synthetic_data/factories.py +++ b/neofox/tests/synthetic_data/factories.py @@ -1,7 +1,7 @@ from typing import List from Bio import SeqIO -from Bio.Alphabet.IUPAC import IUPACData +from Bio.Data import IUPACData from faker.providers.address import Provider from neofox.exceptions import NeofoxDataValidationException diff --git a/neofox/tests/unit_tests/test_differential_binding.py b/neofox/tests/unit_tests/test_differential_binding.py index b5e2a461..c71f2cc2 100755 --- a/neofox/tests/unit_tests/test_differential_binding.py +++ b/neofox/tests/unit_tests/test_differential_binding.py @@ -102,3 +102,9 @@ def test_affinity_threshold(self): score_mutation=1030, score_wild_type=1030 ) self.assertIsNotNone(result) + + result = diffbdg_calculator.dai( + score_mutation=50, score_wild_type=None + ) + self.assertIsNone(None) + diff --git a/neofox/tests/unit_tests/test_runner.py b/neofox/tests/unit_tests/test_runner.py index d8cdb6b5..2b418724 100755 --- a/neofox/tests/unit_tests/test_runner.py +++ b/neofox/tests/unit_tests/test_runner.py @@ -28,7 +28,10 @@ def setUp(self): def test_runner(self): output, errors = self.runner.run_command(cmd=["python", "-V"]) - self.assertTrue("Python 3.7" in output or "Python 3.8" in output, "Output:" + output) + self.assertTrue( + any([pyv in output for pyv in ["Python 3.9", "Python 3.10", "Python 3.11"]]), + "Output:" + output + ) self.assertTrue(len(errors) == 0) def test_runner_failure(self): diff --git a/neofox/tests/unit_tests/test_tcell_predictor.py b/neofox/tests/unit_tests/test_tcell_predictor.py deleted file mode 100755 index 3ad1a908..00000000 --- a/neofox/tests/unit_tests/test_tcell_predictor.py +++ /dev/null @@ -1,83 +0,0 @@ -# -# Copyright (c) 2020-2030 Translational Oncology at the Medical Center of the Johannes Gutenberg-University Mainz gGmbH. -# -# This file is part of Neofox -# (see https://github.com/tron-bioinformatics/neofox). -# -# This program is free software: you can redistribute it and/or modify -# it under the terms of the GNU General Public License as published by -# the Free Software Foundation, either version 3 of the License, or -# (at your option) any later version. -# -# This program is distributed in the hope that it will be useful, -# but WITHOUT ANY WARRANTY; without even the implied warranty of -# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the -# GNU General Public License for more details. -# -# You should have received a copy of the GNU General Public License -# along with this program. If not, see .# -from unittest import TestCase - -from neofox.model.neoantigen import PredictedEpitope -from neofox.published_features.Tcell_predictor.tcellpredictor_wrapper import ( - TcellPrediction, -) - - -class TestTCellPredictor(TestCase): - def setUp(self) -> None: - self.tcell_predictor = TcellPrediction() - - # TODO: Franzi maybe you can add some more sensible tests here? - - def test_non_existing_gene(self): - result = TcellPrediction().calculate_tcell_predictor_score( - gene="BLAH", - epitope=PredictedEpitope(wild_type_peptide="BLAHBLAH", mutated_peptide="blaaaah", affinity_mutated=5) - ) - self.assertEqual(None, result) - - def test_empty_gene(self): - result = TcellPrediction().calculate_tcell_predictor_score( - gene=None, - epitope=PredictedEpitope(wild_type_peptide="BLAHBLAH", mutated_peptide="blaaaah", affinity_mutated=5) - ) - self.assertEqual(None, result) - result = TcellPrediction().calculate_tcell_predictor_score( - gene="", - epitope=PredictedEpitope(wild_type_peptide="BLAHBLAH", mutated_peptide="blaaaah", affinity_mutated=5) - ) - self.assertEqual(None, result) - result = TcellPrediction().calculate_tcell_predictor_score( - gene=" ", - epitope=PredictedEpitope(wild_type_peptide="BLAHBLAH", mutated_peptide="blaaaah", affinity_mutated=5) - ) - self.assertEqual(None, result) - - def test_existing_gene_with_too_short_epitope(self): - result = TcellPrediction().calculate_tcell_predictor_score( - gene="BRCA2", - epitope=PredictedEpitope(wild_type_peptide="CCCCCC", mutated_peptide="CCVCCC", affinity_mutated=5) - ) - self.assertEqual(None, result) - - def test_existing_gene_with_too_long_epitope(self): - result = TcellPrediction().calculate_tcell_predictor_score( - gene="BRCA2", - epitope=PredictedEpitope(wild_type_peptide="CCCCCCCCCC", mutated_peptide="CCCCCVCCCC", affinity_mutated=5) - ) - self.assertEqual(None, result) - - def test_existing_gene(self): - result = TcellPrediction().calculate_tcell_predictor_score( - gene="BRCA2", - epitope=PredictedEpitope(wild_type_peptide="CCCCCCCCC", mutated_peptide="CCCCVCCCC", affinity_mutated=5) - ) - self.assertAlmostEqual(0.30162944008956233, float(result)) - - def test_rare_aminoacid(self): - result = TcellPrediction().calculate_tcell_predictor_score( - gene="BRCA2", - epitope=PredictedEpitope(wild_type_peptide="CCCCCCCCC", mutated_peptide="CCCCUCCCC", affinity_mutated=5) - ) - self.assertIsNone(result) diff --git a/poetry.lock b/poetry.lock index 7070c0df..d5d06524 100644 --- a/poetry.lock +++ b/poetry.lock @@ -19,36 +19,38 @@ compiler = ["black", "jinja2", "protobuf"] [[package]] name = "biopython" -version = "1.76" +version = "1.83" description = "Freely available tools for computational molecular biology." optional = false -python-versions = "*" +python-versions = ">=3.8" files = [ - {file = "biopython-1.76-cp27-cp27m-macosx_10_6_intel.whl", hash = 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(>=1.9)", "sphinx (>=3.5)", "sphinx-lint"] +test = ["big-O", "importlib-resources", "jaraco.functools", "jaraco.itertools", "jaraco.test", "more-itertools", "pytest (>=6,!=8.1.*)", "pytest-checkdocs (>=2.4)", "pytest-cov", "pytest-enabler (>=2.2)", "pytest-ignore-flaky", "pytest-mypy", "pytest-ruff (>=0.2.1)"] [metadata] lock-version = "2.0" -python-versions = ">=3.7.1,<3.9" -content-hash = "a76af5d0ef601ea1dcd8eb6eeb37e93c3fb3164b1cfcfba7254fe648023b5e18" +python-versions = "~3.11" +content-hash = "9b853d99cd387e56e708f1e9fca1f693f05e3f8d7d61087722e34dc08a7c21a8" diff --git a/pyproject.toml b/pyproject.toml index d14add93..77216a13 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -1,6 +1,6 @@ [tool.poetry] name = "neofox" -version = "1.1.1.dev8" +version = "1.1.1.dev9" description = "NEOantigen Feature tOolboX" authors = [ "TRON - Translational Oncology at the University Medical Center", @@ -16,22 +16,21 @@ packages = [ # main dependency group [tool.poetry.dependencies] -python = ">=3.7.1,<3.9" -biopython = "1.76" +python = "~3.11" +biopython = ">1.80" mock = "~4.0.3" -pandas = ">=1.3.5,<1.4" -numpy = ">=1.21,<1.22" -scipy = ">=1.7.3,<1.8" -scikit-learn = "0.22.1" +pandas = ">=2.1,<2.3" +numpy = ">=1.24,<1.27" +scipy = ">=1.10.0" logzero = ">=1.5.0" python-dotenv = "~0.12.0" betterproto = "~1.2.5" -pysam = "~0.19.1" -dask = ">=2022.2.0,<2023.0.0" -distributed = ">=2022.2.0,<2023.0.0" +pysam = ">0.20" +dask = ">=2024.0.0,<=2024.6.2" +distributed = ">=2024.0.0,<=2024.6.2" faker = "~13.13.0" xmltodict = "~0.12.0" -cython = "0.29.33" +cython = ">3" #[tool.poetry.group.doc] #optional = true diff --git a/tox.ini b/tox.ini index c4f96075..ee6f51c7 100644 --- a/tox.ini +++ b/tox.ini @@ -1,5 +1,5 @@ [tox] -envlist = {py37, py38} +envlist = {py311} isolated_build = True [testenv] From b8245fe0168bbefc0f5d8c226e7375ef05766000 Mon Sep 17 00:00:00 2001 From: Franziska Lang Date: Wed, 3 Jul 2024 16:16:31 +0200 Subject: [PATCH 53/66] make patient data optional in epitope mode --- neofox/command_line.py | 8 +++++--- neofox/neofox_epitope.py | 11 ++++++----- 2 files changed, 11 insertions(+), 8 deletions(-) diff --git a/neofox/command_line.py b/neofox/command_line.py index 68c11c38..67e77298 100755 --- a/neofox/command_line.py +++ b/neofox/command_line.py @@ -373,10 +373,12 @@ def neofox_epitope_cli(): def _read_data_epitopes( input_file, patients_data, mhc_database: MhcDatabase, organism: str) -> Tuple[List[PredictedEpitope], List[Patient]]: - # parse patient data + # parse optional patient data + patients = None logger.info("Parsing patients data from: {}".format(patients_data)) - patients = ModelConverter.parse_patients_file(patients_data, mhc_database) - logger.info("Loaded {} patients".format(len(patients))) + if patients_data: + patients = ModelConverter.parse_patients_file(patients_data, mhc_database) + logger.info("Loaded {} patients".format(len(patients))) # parse the neoantigen candidate data diff --git a/neofox/neofox_epitope.py b/neofox/neofox_epitope.py index 5ec65de2..0af4de8a 100755 --- a/neofox/neofox_epitope.py +++ b/neofox/neofox_epitope.py @@ -71,11 +71,12 @@ def __init__( self.self_similarity = SelfSimilarityCalculator() self.num_cpus = num_cpus - # validates patients - self.patients = {} - for patient in patients: - ModelValidator.validate_patient(patient, organism=self.reference_folder.organism) - self.patients[patient.identifier] = patient + # validates optional patient object + if patients: + self.patients = {} + for patient in patients: + ModelValidator.validate_patient(patient, organism=self.reference_folder.organism) + self.patients[patient.identifier] = patient if neoepitopes is None or len(neoepitopes) == 0: raise NeofoxConfigurationException("Missing input data to run Neofox") From d1c1f3abcc75b105773b42907249d22d45149bcc Mon Sep 17 00:00:00 2001 From: Franziska Lang Date: Wed, 3 Jul 2024 17:06:47 +0200 Subject: [PATCH 54/66] update documentation --- README.md | 3 +-- docs/source/03_01_input_data.md | 42 ++++++++++++++++---------------- docs/source/03_02_output_data.md | 14 ++++------- docs/source/03_03_usage.md | 11 ++++++--- pyproject.toml | 2 +- 5 files changed, 36 insertions(+), 36 deletions(-) diff --git a/README.md b/README.md index 9f9cac80..8a49343d 100755 --- a/README.md +++ b/README.md @@ -8,7 +8,7 @@ NeoFox annotates neoantigen candidate sequences with published neoantigen features. -For a detailed documentation, please check out [https://neofox.readthedocs.io](https://neofox.readthedocs.io/) +**For a detailed documentation, please check out [https://neofox.readthedocs.io](https://neofox.readthedocs.io/)** If you use NeoFox, please cite the following publication: Franziska Lang, Pablo Riesgo-Ferreiro, Martin Löwer, Ugur Sahin, Barbara Schrörs, **NeoFox: annotating neoantigen candidates with neoantigen features**, Bioinformatics, Volume 37, Issue 22, 15 November 2021, Pages 4246–4247, https://doi.org/10.1093/bioinformatics/btab344 @@ -41,7 +41,6 @@ NeoFox covers the following neoantigen features and prediction algorithms: | Recognition potential § | Łuksza et al, 2017, Nature; Balachandran et al, 2017, Nature | https://doi.org/10.1038/nature24473 , https://doi.org/10.1038/nature24462 | | Vaxrank | Rubinsteyn, 2017, Front Immunol | https://doi.org/10.3389/fimmu.2017.01807 | | Priority score | Bjerregaard et al, 2017, Cancer Immunol Immunother. | https://doi.org/10.1007/s00262-017-2001-3 | -| Tcell predictor | Besser et al, 2019, Journal for ImmunoTherapy of Cancer | https://doi.org/10.1186/s40425-019-0595-z | | PRIME § | Schmidt et al., 2021, Cell Reports Medicine | https://doi.org/10.1016/j.xcrm.2021.100194 | | HEX § | Chiaro et al., 2021, Cancer Immunology Research | https://doi.org/10.1158/2326-6066.CIR-20-0814 | diff --git a/docs/source/03_01_input_data.md b/docs/source/03_01_input_data.md index 69ce881b..2452c484 100644 --- a/docs/source/03_01_input_data.md +++ b/docs/source/03_01_input_data.md @@ -26,13 +26,13 @@ This is an dummy example of a table with neoantigen candidates in tabular format | BRCA2 | AAAAAAAAAAAAAKAAAAAAAAAAAAA | AAAAAAAAAAAAACAAAAAAAAAAAAA | Ptx | 7.942 | 0.85 | 0.34 | some_value | some_value | where: -- `gene`: the HGNC gene symbol. (This field is not required for neoantigen candidates derived from other sources than SNVs) -- `mutatedXmer`: the neoantigen candidate sequence, i.e. the mutated amino acid sequence. In case of SNVs, the mutation should be located in the middle. We advise that the point mutation is flanked by 13 amino acid on both sites (IUPAC 1 respecting casing, eg: A) to cover both MHC I and MHC II neopeptides -- `wildTypeXmer`: the equivalent non-mutated amino acid sequence (IUPAC 1 respecting casing, eg: A). This field shall be empty, specially in the case of neoantigen candidates derived from other sources than SNVs. -- `patientIdentifier`: the patient identifier -- `rnaExpression`: RNA expression. (**optional**) (see *NOTE*) This value can be in any format chosen by the user (e.g. TPM, RPKM) but it is recommended to be consistent for data that should be compared. -- `rnaVariantAlleleFrequency`: the variant allele frequency (VAF) calculated from the RNA (**optional**) -- `dnaVariantAlleleFrequency`: the VAF calculated from the DNA. (**optional**) +- **gene**: the HGNC gene symbol. (This field is not required for neoantigen candidates derived from other sources than SNVs) +- **mutatedXmer**: the neoantigen candidate sequence, i.e. the mutated amino acid sequence. In case of SNVs, the mutation should be located in the middle. We advise that the point mutation is flanked by 13 amino acid on both sites (IUPAC 1 respecting casing, eg: A) to cover both MHC I and MHC II neopeptides +- **wildTypeXmer**: the equivalent non-mutated amino acid sequence (IUPAC 1 respecting casing, eg: A). This field shall be empty, specially in the case of neoantigen candidates derived from other sources than SNVs. +- **patientIdentifier**: the patient identifier +- **rnaExpression**: RNA expression. (see *NOTE*) This value can be in any format chosen by the user (e.g. TPM, RPKM) but it is recommended to be consistent for data that should be compared. (*optional*) +- `rnaVariantAlleleFrequency`: the variant allele frequency (VAF) calculated from the RNA (*optional*) +- `dnaVariantAlleleFrequency`: the VAF calculated from the DNA. (*optional*) **NOTE:** @@ -52,20 +52,20 @@ This is an dummy example of a table with neoepitope candidates in tabular format | BRCA2 | AAAAAAAAAAAAAKAAAAAAAAAAAAA | AAAAAAAAAAAAACAAAAAAAAAAAAA | | | Ptx | 7.942 | 0.85 | 0.34 | where: -- `mutatedPeptide`: the neoepitope candidate sequence, i.e. the mutated amino acid sequence. MHC-I neoepitopes should have a length between 8 and 14 amino acids, MHC-II neoepitopes should have a length between 9 and 20000 amino acids. -- `wildTypePeptide`: the equivalent non-mutated amino acid sequence (IUPAC 1 respecting casing, eg: A). This field shall be empty, specially in the case of neoepitope candidates derived from other sources than SNVs. -- `alleleMhcI`: the MHC-I allele to which this neoepitope is linked (**optional**) -- `isoformMhcII`: the MHC-II isoform to which this neoepitope is linked (**optional**) -- `patientIdentifier`: the patient identifier (**only required if alleleMhcI and isoformMhcII are not provided**) -- `gene`: the HGNC gene symbol. (This field is optional) -- `rnaExpression`: RNA expression. (**optional**) (see *NOTE*) This value can be in any format chosen by the user (e.g. TPM, RPKM) but it is recommended to be consistent for data that should be compared. -- `rnaVariantAlleleFrequency`: the variant allele frequency (VAF) calculated from the RNA (**optional**) -- `dnaVariantAlleleFrequency`: the VAF calculated from the DNA. (**optional**) +- **mutatedPeptide**: the neoepitope candidate sequence, i.e. the mutated amino acid sequence. MHC-I neoepitopes should have a length between 8 and 14 amino acids, MHC-II neoepitopes should have a length between 9 and 20000 amino acids. +- **wildTypePeptide**: the equivalent non-mutated amino acid sequence (IUPAC 1 respecting casing, eg: A). This field shall be empty, specially in the case of neoepitope candidates derived from other sources than SNVs. +- **alleleMhcI**: the MHC-I allele to which this neoepitope is linked (*optional*) +- **isoformMhcII**: the MHC-II isoform to which this neoepitope is linked (*optional*) +- **patientIdentifier**: the patient identifier (*only required if alleleMhcI and isoformMhcII are not provided*) +- **gene**: the HGNC gene symbol. (This field is optional) +- **rnaExpression**: RNA expression. (*optional*) (see *NOTE*) This value can be in any format chosen by the user (e.g. TPM, RPKM) but it is recommended to be consistent for data that should be compared. +- **rnaVariantAlleleFrequency**: the variant allele frequency (VAF) calculated from the RNA (*optional*) +- **dnaVariantAlleleFrequency**: the VAF calculated from the DNA. (*optional*) **NOTE:** - Neoepitopes with a value for `alleleMhcI` are considered MHC-I neoepitopes, likewise neoepitopes with a value for `isoformMhcII` are considered MHC-II neoepitopes. Both fields cannot be provided for the same neoepitope. -- If none of `alleleMhcI` and `isoformMhcII` are provided then the `patientIdentifier` is required and one neoepitope sharing the same sequence will be annotated for each MHC-I allele and MHC-II isoform according to the patient HLA type. +- If none of `alleleMhcI` and `isoformMhcII` are provided then the `patientIdentifier` is required and one neoepitope sharing the same sequence will be annotated for each MHC-I allele and MHC-II isoform according to the patient HLA type provided in the patient data. - Neofox annotates gene expression in TCGA cohort indicated in the `tumorType` in the patient data (see below) which might be helpful if rnaExpression is unknown. Please, not that this does not work for mouse data. @@ -100,10 +100,10 @@ This is an dummy example of a patient file in tabular format: | Pty | HLA-A\*02:01,HLA-A\*30:01,HLA-B\*07:34,HLA-B\*44:03,HLA-C\*07:02,HLA-C*07:02 | HLA-DRB1\*04:02,HLA-DRB1\*08:01,HLA-DQA1\*03:01,HLA-DQA1\*04:01,HLA-DQB1\*03:02,HLA-DQB1\*14:01,HLA-DPA1\*01:03,HLA-DPA1\*02:01,HLA-DPB1\*02:01,HLA-DPB1*04:01 | HNSC | where: -- `identifier`: the patient identifier -- `mhcIAlleles`: comma separated MHC I alleles of the patient for HLA-A, HLA-B and HLA-C. If homozygous, the allele should be added twice. -- `mhcIIAlleles`: comma separated MHC II alleles of the patient for HLA-DRB1, HLA-DQA1, HLA-DQB1, HLA-DPA1 and HLA-DPB1. If homozygous, the allele should be added twice. -- `tumorType`: tumour entity in TCGA study abbreviation format (https://gdc.cancer.gov/resources-tcga-users/tcga-code-tables/tcga-study-abbreviations). This field is required for imputation with gene expression and at the moment the following tumor types are supported: +- **identifier**: the patient identifier +- **mhcIAlleles**: comma separated MHC I alleles of the patient for HLA-A, HLA-B and HLA-C. If homozygous, the allele should be added twice. +- **mhcIIAlleles**: comma separated MHC II alleles of the patient for HLA-DRB1, HLA-DQA1, HLA-DQB1, HLA-DPA1 and HLA-DPB1. If homozygous, the allele should be added twice. +- **tumorType**: tumour entity in TCGA study abbreviation format (https://gdc.cancer.gov/resources-tcga-users/tcga-code-tables/tcga-study-abbreviations). This field is *optional* and only required for imputation with gene expression and at the moment the following tumor types are supported: | Study Name | Abbreviation | |--------------------------------------------------------------------|-------------------| diff --git a/docs/source/03_02_output_data.md b/docs/source/03_02_output_data.md index dcf6547d..445ec537 100755 --- a/docs/source/03_02_output_data.md +++ b/docs/source/03_02_output_data.md @@ -96,7 +96,6 @@ The following table describes each of the annotations in the output: | GeneratorRate_CDN_MHCII | number of neoepitope candidates with MHC II binding rank score < 1 per neoantigen canidate | Generator rate | | GeneratorRate_ADN_MHCII | number of neoepitope candidates with MHC II binding rank score < 4 per neoantigen candidate 4x better rank in comparison to corresponding WT peptide | Generator rate | | GeneratorRate_MHCII | sum of `GeneratorRate_CDN_MHCII` and `GeneratorRate_ADN_MHCII` | Generator rate | -| Tcell_predictor | output score of T cell predictor model | Tcell predictor | | ImprovedBinder_MHCI | ratio of `NetMHCpan_MHCI_rank_bestRankWT` and `NetMHCpan_MHCI_rank_bestRank` > 1.2 | self-similarity | | Selfsimilarity_MHCI_conserved_binder | score representing the similarity between `NetMHCpan_bestRank_peptide` and `NetMHCpan_bestRank_peptideWT` For conservered binder only | self-similarity | | Selfsimilarity_MHCI | score representing the similarity between `NetMHCpan_bestRank_peptide` and `NetMHCpan_bestRank_peptide` | self-similarity | @@ -137,16 +136,14 @@ prefix can be set with `--output-prefix`. ### Tabular format An output table with the suffix "*_neoantigen_candidates_annotated.tsv*" is created. -This table contains the neoantigen candidates information, the neoantigen annotations and if some user-specific additional columns -were provided in the input table, these external annotations. +This table contains the neoantigen candidates information, the neoantigen annotations and external annotations if they were present provided in the input table. This is a dummy example: -| dnaVariantAlleleFrequency | gene | imputedGeneExpression | mutatedXmer | position | wildTypeXmer | patientIdentifier | rnaExpression | rnaVariantAlleleFrequency | +-13_AA_(SNV)_/_-15_AA_to_STOP_(INDEL) | ADN_MHCI | ADN_MHCII | Amplitude_MHCII_rank | Amplitude_MHCI_affinity | Amplitude_MHCI_affinity_9mer | Best_affinity_MHCII_allele | Best_affinity_MHCII_allele_WT | Best_affinity_MHCII_epitope | Best_affinity_MHCII_epitope_WT | Best_affinity_MHCII_score | Best_affinity_MHCII_score_WT | Best_affinity_MHCI_9mer_allele | Best_affinity_MHCI_9mer_allele_WT | Best_affinity_MHCI_9mer_anchor_mutated | Best_affinity_MHCI_9mer_epitope | Best_affinity_MHCI_9mer_epitope_WT | Best_affinity_MHCI_9mer_position_mutation | Best_affinity_MHCI_9mer_score | Best_affinity_MHCI_9mer_score_WT | Best_affinity_MHCI_allele | Best_affinity_MHCI_allele_WT | Best_affinity_MHCI_epitope | Best_affinity_MHCI_epitope_WT | Best_affinity_MHCI_score | Best_affinity_MHCI_score_WT | Best_rank_MHCII_score | Best_rank_MHCII_score_WT | Best_rank_MHCII_score_allele | Best_rank_MHCII_score_allele_WT | Best_rank_MHCII_score_epitope | Best_rank_MHCII_score_epitope_WT | Best_rank_MHCI_9mer_allele | Best_rank_MHCI_9mer_allele_WT | Best_rank_MHCI_9mer_epitope | Best_rank_MHCI_9mer_epitope_WT | Best_rank_MHCI_9mer_score | Best_rank_MHCI_9mer_score_WT | Best_rank_MHCI_score | Best_rank_MHCI_score_WT | Best_rank_MHCI_score_allele | Best_rank_MHCI_score_allele_WT | Best_rank_MHCI_score_epitope | Best_rank_MHCI_score_epitope_WT | CDN_MHCI | CDN_MHCII | DAI_MHCI_affinity_cutoff500nM | Dissimilarity_MHCI_cutoff500nM | Expression_mutated_transcript | Generator_rate | IEDB_Immunogenicity_MHCI_cutoff500nM | Improved_Binder_MHCI | MixMHC2pred_best_allele | MixMHC2pred_best_peptide | MixMHC2pred_best_rank | MixMHCpred_best_allele | MixMHCpred_best_peptide | MixMHCpred_best_rank | MixMHCpred_best_score | Number_of_mismatches_MCHI | PHBR-I | PHBR-II | Pathogensimiliarity_MHCI_affinity_9mer | Priority_score | Recognition_Potential_MHCI_affinity_9mer | Selfsimilarity_MHCI_conserved_binder | Tcell_predictor_score_cutoff500nM | VAF_in_RNA | VAF_in_tumor | [WT]_+-13_AA_(SNV)_/_-15_AA_to_STOP_(INDEL) | mutation_not_found_in_proteome | patient | substitution | transcript_expression | vaxrank_binding_score | vaxrank_total_score | -|---------------------------|-------|------------------------|-----------------------------|-----------|-----------------------------|-------------------|---------------|---------------------------|----------------------------------------|----------|-----------|----------------------|-------------------------|------------------------------|----------------------------|-------------------------------|-----------------------------|--------------------------------|---------------------------|------------------------------|--------------------------------|-----------------------------------|----------------------------------------|---------------------------------|------------------------------------|-------------------------------------------|-------------------------------|----------------------------------|---------------------------|------------------------------|----------------------------|-------------------------------|--------------------------|-----------------------------|-----------------------|--------------------------|------------------------------|---------------------------------|-------------------------------|----------------------------------|----------------------------|-------------------------------|-----------------------------|--------------------------------|---------------------------|------------------------------|----------------------|-------------------------|-----------------------------|--------------------------------|------------------------------|---------------------------------|----------|-----------|-------------------------------|--------------------------------|-------------------------------|----------------|--------------------------------------|----------------------|-------------------------|--------------------------|-----------------------|------------------------|-------------------------|----------------------|-----------------------|---------------------------|---------|---------|----------------------------------------|----------------|------------------------------------------|--------------------------------------|-----------------------------------|------------|--------------|---------------------------------------------|--------------------------------|---------|--------------|-----------------------|-----------------------|---------------------| - | 0.294 | BRCA2 | 0.5 | AAAAAAAAAAAAAFAAAAAAAAAAAAA | 14 | AAAAAAAAAAAAALAAAAAAAAAAAAA | Ptx | 0.51950689 | 0.857 | AAAAAAAAAAAAAFAAAAAAAAAAAAA | 0 | 1 | 28 | 0.88723 | 0.88723 | HLA-DQA10401-DQB10402 | HLA-DQA10401-DQB10402 | AAAAFAAAAAAAAAA | AAAALAAAAAAAAAA | 251.77 | 513.02 | HLA-C*16:01 | HLA-C*16:01 | 1 | AAAAAAAAF | AAAAAAAAL | 9 | 24.3 | 21.7 | HLA-C*16:01 | HLA-C*16:01 | AAAAAAAAF | AAAAAAAAL | 24.3 | 21.7 | 0.05 | 1.4 | HLA-DQA10301-DQB10402 | HLA-DQA10301-DQB10402 | AAAAFAAAAAAAAAA | AAAALAAAAAAAAAA | HLA-C*16:01 | HLA-C*16:01 | AAAAAAAAF | AAAAAAAAL | 0.0592 | 0.0493 | 0.0592 | 0.0493 | HLA-C*16:01 | HLA-C*16:01 | AAAAAAAAF | AAAAAAAAL | 1 | 1 | -2.6 | 1 | 0.44522 | 1 | 0.18288 | 0 | DPA1_01_03__DPB1_04_01 | AAAAFAAAAAAAAAAA | 0.997 | B0702 | AAAAAAAAF | 0.1 | 0.50487 | 1 | 0.31193 | 0.21892 | 0 | 0.07017 | 0 | 0.99178271 | 0.40327581 | 0.857 | 0.294 | AAAAAAAAAAAAALAAAAAAAAAAAAA | 1 | Ptx | I547T | 0.51950689 | 3.7689 | 1.678 | -| 0.173 | BRCA2 | 0.5 | AAAAAAAAAAAAAMAAAAAAAAAAAAA | 14 | AAAAAAAAAAAAARAAAAAAAAAAAAA | Ptx | 0.71575659 | 0.556 | AAAAAAAAAAAAAMAAAAAAAAAAAAA | 1 | 1 | 10 | 90.685 | 90.685 | HLA-DQA10401-DQB10402 | HLA-DQA10401-DQB10402 | AAAAAAAAAMAAAAA | AAAAAAAAARAAAAA | 421.53 | 554.92 | HLA-C*16:01 | HLA-C*16:01 | 1 | AAAAAAAAM | AAAAAAAAR | 9 | 24.1 | 6346.9 | HLA-C*16:01 | HLA-C*16:01 | AAAAAAAAM | AAAAAAAAR | 24.1 | 6346.9 | 0.25 | 2.5 | HLA-DQA10401-DQB10302 | HLA-DQA10401-DQB10302 | AAAAAAAAAAMAAAA | AAAAAAAAAARAAAA | HLA-C*16:01 | HLA-C*16:01 | AAAAAAAAM | AAAAAAAAR | 0.0587 | 8.9317 | 0.0587 | 8.9317 | HLA-C*16:01 | HLA-C*16:01 | AAAAAAAAM | AAAAAAAAR | 1 | 1 | 6322.8 | 1 | 0.39796 | 1 | 0.18288 | 1 | DPA1_01_03__DPB1_04_01 | AAAAMAAAAAAAAAAA | 2.44 | B0702 | AAAAAAAAM | 0.07 | 0.5444 | 1 | 0.29303 | 1.5594 | 0 | 0.10626 | 0 | NA | 0.46452844 | 0.556 | 0.173 | AAAAAAAAAAAAARAAAAAAAAAAAAA | 1 | Ptx | E135S | 0.71575659 | 3.8741 | 1.5417 | - +| patientIdentifier | gene | mutatedXmer | wildTypeXmer | position | dnaVariantAlleleFrequency | rnaVariantAlleleFrequency | rnaExpression | imputedGeneExpression | Alternatively_defined_neopeptide_MHCI | Alternatively_defined_neopeptide_MHCII | Amplitude_MHCII_bestRank | Amplitude_MHCI_bestAffinity | Amplitude_MHCI_bestAffinity9mer | Classically_defined_neopeptide_MHCI | Classically_defined_neopeptide_MHCII | DAI_MHCI_bestAffinity | Dissimilarity_MHCI | Dissimilarity_MHCII | GeneratorRate_ADN_MHCI | GeneratorRate_ADN_MHCII | GeneratorRate_CDN_MHCI | GeneratorRate_CDN_MHCII | GeneratorRate_MHCI | GeneratorRate_MHCII | HexAlignmentScore_MHCI | HexAlignmentScore_MHCII | IEDB_Immunogenicity_MHCI | IEDB_Immunogenicity_MHCII | Improved_Binder_MHCI | MixMHC2pred_bestRank_allele | MixMHC2pred_bestRank_peptide | MixMHC2pred_bestRank_rank | MixMHCpred_bestScore_allele | MixMHCpred_bestScore_peptide | MixMHCpred_bestScore_rank | MixMHCpred_bestScore_score | Mutated_imputedGeneExpression_fromDNA | Mutated_imputedGeneExpression_fromRNA | Mutated_rnaExpression_fromDNA | Mutated_rnaExpression_fromRNA | NetMHCIIpan_bestAffinity_Of | NetMHCIIpan_bestAffinity_affinity | NetMHCIIpan_bestAffinity_affinityWT | NetMHCIIpan_bestAffinity_allele | NetMHCIIpan_bestAffinity_core | NetMHCIIpan_bestAffinity_coreRel | NetMHCIIpan_bestAffinity_peptide | NetMHCIIpan_bestAffinity_peptideWT | NetMHCIIpan_bestRank_Of | NetMHCIIpan_bestRank_allele | NetMHCIIpan_bestRank_core | NetMHCIIpan_bestRank_coreRel | NetMHCIIpan_bestRank_peptide | NetMHCIIpan_bestRank_peptideWT | NetMHCIIpan_bestRank_rank | NetMHCIIpan_bestRank_rankWT | NetMHCpan_bestAffinity9mer_affinity | NetMHCpan_bestAffinity9mer_affinityWT | NetMHCpan_bestAffinity9mer_allele | NetMHCpan_bestAffinity9mer_anchorMutated | NetMHCpan_bestAffinity9mer_peptide | NetMHCpan_bestAffinity9mer_peptideWT | NetMHCpan_bestAffinity9mer_positionMutation | NetMHCpan_bestAffinity_Gl | NetMHCpan_bestAffinity_Gp | NetMHCpan_bestAffinity_Icore | NetMHCpan_bestAffinity_Of | NetMHCpan_bestAffinity_affinity | NetMHCpan_bestAffinity_affinityWT | NetMHCpan_bestAffinity_allele | NetMHCpan_bestAffinity_core | NetMHCpan_bestAffinity_peptide | NetMHCpan_bestAffinity_peptideWT | NetMHCpan_bestRank9mer_allele | NetMHCpan_bestRank9mer_peptide | NetMHCpan_bestRank9mer_peptideWT | NetMHCpan_bestRank9mer_rank | NetMHCpan_bestRank9mer_rankWT | NetMHCpan_bestRank_Gl | NetMHCpan_bestRank_Gp | NetMHCpan_bestRank_Icore | NetMHCpan_bestRank_Of | NetMHCpan_bestRank_allele | NetMHCpan_bestRank_core | NetMHCpan_bestRank_peptide | NetMHCpan_bestRank_peptideWT | NetMHCpan_bestRank_rank | NetMHCpan_bestRank_rankWT | Number_of_mismatches_MCHI | PHBR_I | PHBR_II | PRIME_best_allele | PRIME_best_peptide | PRIME_best_rank | PRIME_best_score | Pathogensimiliarity_MHCII_bestAffinity | Pathogensimiliarity_MHCI_bestAffinity | Pathogensimiliarity_MHCI_bestAffinity9mer | Priority_score_fromDNA | Priority_score_fromRNA | Priority_score_imputed_fromDNA | Priority_score_imputed_fromRNA | RecognitionPotential_MHCI_bestAffinity | RecognitionPotential_MHCI_bestAffinity9mer | Selfsimilarity_MHCI | Selfsimilarity_MHCII | Selfsimilarity_MHCI_conserved_binder | Vaxrank_bindingScore | Vaxrank_totalScore | Vaxrank_totalScore_imputed | mutation_not_found_in_proteome | +|-------------------|-------|-----------------------------|-----------------------------|----------|---------------------------|---------------------------|---------------|-----------------------|---------------------------------------|----------------------------------------|--------------------------|-----------------------------|---------------------------------|-------------------------------------|--------------------------------------|-----------------------|--------------------|---------------------|------------------------|-------------------------|------------------------|-------------------------|--------------------|---------------------|------------------------|-------------------------|--------------------------|---------------------------|----------------------|-----------------------------|------------------------------|---------------------------|-----------------------------|------------------------------|---------------------------|----------------------------|---------------------------------------|---------------------------------------|-------------------------------|-------------------------------|-----------------------------|-----------------------------------|-------------------------------------|---------------------------------|-------------------------------|----------------------------------|----------------------------------|------------------------------------|-------------------------|-----------------------------|---------------------------|------------------------------|------------------------------|--------------------------------|---------------------------|-----------------------------|-------------------------------------|---------------------------------------|-----------------------------------|------------------------------------------|------------------------------------|--------------------------------------|---------------------------------------------|---------------------------|---------------------------|------------------------------|---------------------------|---------------------------------|-----------------------------------|-------------------------------|-----------------------------|--------------------------------|----------------------------------|-------------------------------|--------------------------------|----------------------------------|-----------------------------|-------------------------------|-----------------------|-----------------------|--------------------------|-----------------------|---------------------------|-------------------------|----------------------------|------------------------------|-------------------------|---------------------------|---------------------------|--------|---------|-------------------|--------------------|-----------------|------------------|----------------------------------------|---------------------------------------|-------------------------------------------|------------------------|------------------------|--------------------------------|--------------------------------|----------------------------------------|--------------------------------------------|---------------------|----------------------|--------------------------------------|----------------------|--------------------|----------------------------|--------------------------------| +| Ptx | BRCA2 | AAAAAAAAAAAAALAAAAAAAAAAAAA | AAAAAAAAAAAAAFAAAAAAAAAAAAA | 14 | 0.857 | 0.294 | 0.51950689 | 0.53659963 | 0 | 0 | 0.33333 | 2.3262 | 0.56811 | 0 | 1 | 7165.9 | 1 | 3.00E-05 | 0 | 1 | 0 | 18 | 0 | 19 | 177 | 389 | 0.18182 | 0.36258 | 0 | HLA-DQA1*01:02-DQB1*06:02 | AAAAAAALAAAAAAA | 0.00438 | HLA-B*07:02 | AAALAAAAA | 0.18911 | -0.2706 | 0.45987 | 0.15776 | 0.44522 | 0.15274 | 3 | 14.84 | 21.8 | HLA-DQA1*01:02-DQB1*06:02 | AAAAALAAA | 0.74 | AAAAAAAALAAAAAA | AAAAAAAAFAAAAAA | 3 | HLA-DQA1*03:01-DQB1*06:02 | LAAAAAAAA | 0.75 | AAALAAAAAAAAAAA | AAAFAAAAAAAAAAA | 0.03 | 0.01 | 1534.8 | 1180.8 | HLA-C*03:04 | 0 | AAALAAAAA | AAAFAAAAA | 4 | 1 | 1 | AALAAAAAAA | 0 | 1018.2 | 8184.1 | HLA-A*02:01 | ALAAAAAAA | AALAAAAAAA | AAFAAAAAAA | HLA-B*07:02 | AAALAAAAA | AAAFAAAAA | 4.389 | 5.068 | 0 | 0 | AAALAAAAA | 0 | HLA-B*07:02 | AAALAAAAA | AAALAAAAA | AAAFAAAAA | 4.389 | 5.068 | 1 | 8.5743 | NA | HLA-B*07:02 | AAALAAAAA | 0.114 | 0.10131 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.97634652 | 0.98126377 | 0.97634652 | 0.01301 | 0.00199 | 0.00205 | 1 | +| Ptx | BRCA2 | AAAAAAAAAAAAARAAAAAAAAAAAAA | AAAAAAAAAAAAAMAAAAAAAAAAAAA | 14 | 0.556 | 0.173 | 0.71575659 | 0.53659963 | 0 | 1 | 390 | 5.6409 | 5.6409 | 0 | 1 | 1498 | 1 | 0 | 0 | 18 | 0 | 27 | 0 | 45 | 132 | 409 | 0.19477 | 0.42378 | 1 | HLA-DQA1*01:02-DQB1*06:02 | AAAARAAAAAAAAAA | 0.00438 | HLA-B*07:02 | AAAAAARAA | 0.08828 | 0.04913 | 0.29835 | 0.09283 | 0.39796 | 0.12383 | 3 | 13.55 | 15.81 | HLA-DQA1*01:02-DQB1*06:02 | AAAARAAAA | 0.97 | AAAAAAARAAAAAAA | AAAAAAAMAAAAAAA | 3 | HLA-DPA1*01:03-DPB1*06:01 | RAAAAAAAA | 1 | AAARAAAAAAAAAAA | AAAMAAAAAAAAAAA | 0.01 | 3.9 | 199.38 | 1697.4 | HLA-B*07:02 | 0 | AAAAARAAA | AAAAAMAAA | 6 | 0 | 0 | AAAAARAAA | 0 | 199.38 | 1697.4 | HLA-B*07:02 | AAAAARAAA | AAAAARAAA | AAAAAMAAA | HLA-B*07:02 | AAAAARAAA | AAAAAMAAA | 1.346 | 4.347 | 0 | 0 | AAAAARAAA | 0 | HLA-B*07:02 | AAAAARAAA | AAAAARAAA | AAAAAMAAA | 1.346 | 4.347 | 1 | 4.6189 | NA | HLA-B*07:02 | AAAAAARAA | 0.129 | 0.09563 | 0 | 0 | 0 | 0.32903 | 0.10238 | 0.26268 | 0.08173 | 0 | 0 | 0.9663076 | 0.97299187 | NA | 0.80336 | 0.09948 | 0.07458 | 1 | ### JSON format @@ -341,7 +338,6 @@ The following table describes each of the annotations in the output. MHC-I or MH | Selfsimilarity | score representing the similarity between `rankMutated` and `rankWildType` | self-similarity | | Selfsimilarity_conserved_binder | score representing the similarity between `rankMutated` and `rankWildType` for conserved binder only | self-similarity (only available for MHC-I) | | dissimilarity_score | score reflecting the dissimilarity of `affinityMutated` to the self-proteome | dissimilarity | -| Tcell_predictor_score | output score of T cell predictor model | Tcell predictor (only available for MHC-I) | | amplitude | ratio of `affinityWildType` and `affinityMutated` for MHC-I and `rankWildType` and `rankMutated` for MHC-II | Generator rate | | anchor_mutated | flag indicating if a mutation lies in an anchor position (i.e. position 2 or 9) | anchor/non-anchor (only available for MHC-I) | | hex_alignment_score | the alignment score by HEX for `mutatedSequence` | HEX | diff --git a/docs/source/03_03_usage.md b/docs/source/03_03_usage.md index 6921fab5..cae79a3d 100755 --- a/docs/source/03_03_usage.md +++ b/docs/source/03_03_usage.md @@ -82,18 +82,23 @@ neofox-epitope --input-file neoepitope_candidates.tsv \ where: - `--input-file`: tab-separated values table with neoepitope candidates represented by mutated peptide sequences as described [here](03_01_input_data.md#file-with-neoepitope-candidates) (extensions .txt and .tsv) -- `--patient-data`: a table of tab separated values containing metadata on the patient as described [here](03_01_input_data.md#file-with-patient-information) - `--output-folder`: path to the folder to which the output files should be written +- `--patient-data`: a table of tab separated values containing metadata on the patient as described [here](03_01_input_data.md#file-with-patient-information) (*only required if alleleMhcI and isoformMhcII are not provided*) - `--output-prefix`: prefix for the output files (*optional*) - `--organism`: the organism to which the data corresponds. Possible values: [human, mouse]. Default value: human - `--num-cpus`: number of CPUs to use (*optional*) - `--config`: a config file with the paths to dependencies as shown below (*optional*) - `--verbose`: get detailed logs +**NOTE** +The neoepitope can be run in two ways: + 1. Annotate neoepitope candidate with a given MHC allele. This requires MHC-I or MHC-II allele in in the input file but no patient-data file. + 2. Annotate neoepitope candidate with all MHC alleles given in the patient data. This requires no MHC-I or MHC-II allele in in the input file but a patient-data file similar to the neoantigen mode. + + ## Running from docker -**NOTE: The provided docker recipe is not adapted to Neofox-v1.1.0. Please, use a previous version at the moment if running from docker is required. -The docker recipe will be updated soon.** +**NOTE: The provided docker recipe is not adapted in the current NeoFox version. Please, use a previous version (< Neofox-v1.1.0) at the moment if running from docker is required. The docker recipe will be updated soon.** In order to run the command line in a docker image, all of the above applies but some additional steps are required. diff --git a/pyproject.toml b/pyproject.toml index 77216a13..d0727915 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -1,6 +1,6 @@ [tool.poetry] name = "neofox" -version = "1.1.1.dev9" +version = "1.1.1.dev10" description = "NEOantigen Feature tOolboX" authors = [ "TRON - Translational Oncology at the University Medical Center", From 38afdd6325a237848260f814f75088d584f409be Mon Sep 17 00:00:00 2001 From: Franziska Lang Date: Wed, 3 Jul 2024 17:30:48 +0200 Subject: [PATCH 55/66] fix priority score --- neofox/helpers/epitope_helper.py | 1 - neofox/published_features/priority_score.py | 16 ++++++++-------- 2 files changed, 8 insertions(+), 9 deletions(-) diff --git a/neofox/helpers/epitope_helper.py b/neofox/helpers/epitope_helper.py index 2f66ee3a..69d5b5c8 100755 --- a/neofox/helpers/epitope_helper.py +++ b/neofox/helpers/epitope_helper.py @@ -75,7 +75,6 @@ def number_of_mismatches(epitope_wild_type, epitope_mutation): for aa_mut, aa_wt in zip(epitope_mutation, epitope_wild_type): if aa_mut != aa_wt: p1 += 1 - p1 = int(p1) return p1 @staticmethod diff --git a/neofox/published_features/priority_score.py b/neofox/published_features/priority_score.py index 9042955f..9aed72e6 100755 --- a/neofox/published_features/priority_score.py +++ b/neofox/published_features/priority_score.py @@ -170,8 +170,8 @@ def get_annotations_epitope_mhci(self, epitope: PredictedEpitope, vaf_tumor, tra priority_score_dna = self.calc_priority_score( vaf=vaf_tumor, transcript_gene_expr=transcript_exp, - no_mismatch=EpitopeHelper.get_annotation_by_name( - epitope.neofox_annotations.annotations, name='number_of_mismatches'), + no_mismatch=int(EpitopeHelper.get_annotation_by_name( + epitope.neofox_annotations.annotations, name='number_of_mismatches')), score_mut=epitope.rank_mutated, score_wt=epitope.rank_wild_type, mut_not_in_prot=bool(EpitopeHelper.get_annotation_by_name( @@ -181,8 +181,8 @@ def get_annotations_epitope_mhci(self, epitope: PredictedEpitope, vaf_tumor, tra priority_score_imputed_dna = priority_score_rna = self.calc_priority_score( vaf=vaf_tumor, transcript_gene_expr=gene_exp, - no_mismatch=EpitopeHelper.get_annotation_by_name( - epitope.neofox_annotations.annotations, name='number_of_mismatches'), + no_mismatch=int(EpitopeHelper.get_annotation_by_name( + epitope.neofox_annotations.annotations, name='number_of_mismatches')), score_mut=epitope.rank_mutated, score_wt=epitope.rank_wild_type, mut_not_in_prot=bool(EpitopeHelper.get_annotation_by_name( @@ -192,8 +192,8 @@ def get_annotations_epitope_mhci(self, epitope: PredictedEpitope, vaf_tumor, tra priority_score_rna = self.calc_priority_score( vaf=vaf_rna, transcript_gene_expr=transcript_exp, - no_mismatch=EpitopeHelper.get_annotation_by_name( - epitope.neofox_annotations.annotations, name='number_of_mismatches'), + no_mismatch=int(EpitopeHelper.get_annotation_by_name( + epitope.neofox_annotations.annotations, name='number_of_mismatches')), score_mut=epitope.rank_mutated, score_wt=epitope.rank_wild_type, mut_not_in_prot=bool(EpitopeHelper.get_annotation_by_name( @@ -203,8 +203,8 @@ def get_annotations_epitope_mhci(self, epitope: PredictedEpitope, vaf_tumor, tra priority_score_imputed_rna = self.calc_priority_score( vaf=vaf_rna, transcript_gene_expr=gene_exp, - no_mismatch=EpitopeHelper.get_annotation_by_name( - epitope.neofox_annotations.annotations, name='number_of_mismatches'), + no_mismatch=int(EpitopeHelper.get_annotation_by_name( + epitope.neofox_annotations.annotations, name='number_of_mismatches')), score_mut=epitope.rank_mutated, score_wt=epitope.rank_wild_type, mut_not_in_prot=bool(EpitopeHelper.get_annotation_by_name( From b5c924566e8188522114b6678785f326adc8b8ae Mon Sep 17 00:00:00 2001 From: Franziska Lang Date: Wed, 3 Jul 2024 18:08:43 +0200 Subject: [PATCH 56/66] fix priority score --- .../integration_tests/test_neoantigen_annotator.py | 12 ++++++++++-- .../integration_tests/test_neoepitope_annotator.py | 3 ++- 2 files changed, 12 insertions(+), 3 deletions(-) diff --git a/neofox/tests/integration_tests/test_neoantigen_annotator.py b/neofox/tests/integration_tests/test_neoantigen_annotator.py index 071c35eb..ace58b75 100644 --- a/neofox/tests/integration_tests/test_neoantigen_annotator.py +++ b/neofox/tests/integration_tests/test_neoantigen_annotator.py @@ -73,7 +73,7 @@ def test_neoantigen_annotation_without_wild_type_and_with_all_epitopes(self): def test_neoantigen_annotation_without_predicted_wild_type_and_with_all_epitopes(self): neoantigen = NeoantigenFactory.build_neoantigen( - mutated_xmer="ALSIEFQEPVPASMMKVVQVYADTYT", + mutated_xmer="ELSIEFQEPVPASMMKVVQVYADTQE", patient_identifier="123", gene="BRCA2" ) @@ -82,7 +82,15 @@ def test_neoantigen_annotation_without_predicted_wild_type_and_with_all_epitopes self._assert_neoantigen(annotated_neoantigen, neoantigen) # wild type xmer is still empty! self.assertIsNone(annotated_neoantigen.wild_type_xmer) - self._assert_epitopes(annotated_neoantigen, with_all_epitopes=True) + count_none = 0 + count_valid = 0 + for e in annotated_neoantigen.neoepitopes_mhc_i: + if e: + count_valid = count_valid +1 + else: + count_none = count_none + 1 + self.assertGreater(count_none,0) + self.assertGreater(count_valid,0) def test_neoantigen_annotation_with_vaf_and_without_tx_expression(self): diff --git a/neofox/tests/integration_tests/test_neoepitope_annotator.py b/neofox/tests/integration_tests/test_neoepitope_annotator.py index da11af3d..777ad9d5 100644 --- a/neofox/tests/integration_tests/test_neoepitope_annotator.py +++ b/neofox/tests/integration_tests/test_neoepitope_annotator.py @@ -51,7 +51,8 @@ def test_neoepitope_mhci_without_predicted_wild_type(self): ) annotated_neoepitope = self.annotator.get_annotated_neoepitope(neoepitope=neoepitope) - self.assert_neoepitope_mhci(original_neoepitope=neoepitope, annotated_neoepitope=annotated_neoepitope) + self.assertIsNotNone(annotated_neoepitope.rank_mutated) + self.assertIsNone(annotated_neoepitope.rank_wild_type) def test_neoepitope_mhci_9mer_with_frequencies_and_gene(self): """ From 0fe1e78ec3c66a557e10e69f700d65b9aa219bd2 Mon Sep 17 00:00:00 2001 From: Franziska Lang Date: Wed, 3 Jul 2024 18:14:25 +0200 Subject: [PATCH 57/66] fix integration tests --- neofox/tests/integration_tests/test_neoantigen_annotator.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/neofox/tests/integration_tests/test_neoantigen_annotator.py b/neofox/tests/integration_tests/test_neoantigen_annotator.py index ace58b75..71005adb 100644 --- a/neofox/tests/integration_tests/test_neoantigen_annotator.py +++ b/neofox/tests/integration_tests/test_neoantigen_annotator.py @@ -85,7 +85,7 @@ def test_neoantigen_annotation_without_predicted_wild_type_and_with_all_epitopes count_none = 0 count_valid = 0 for e in annotated_neoantigen.neoepitopes_mhc_i: - if e: + if e.wild_type_peptide: count_valid = count_valid +1 else: count_none = count_none + 1 From 6bce707d7cbf2061e5b81d1b33fc702684bc6ece Mon Sep 17 00:00:00 2001 From: Franziska Lang Date: Thu, 4 Jul 2024 08:19:06 +0200 Subject: [PATCH 58/66] update test data --- ...est_mhcII_epitope_candidates_annotated.tsv | 189 ++++++++---------- ...test_mhcI_epitope_candidates_annotated.tsv | 4 +- .../test_neoantigen_candidates_annotated.json | 2 +- .../test_neoantigen_candidates_annotated.tsv | 6 +- 4 files changed, 90 insertions(+), 111 deletions(-) diff --git a/docs/source/_static/test_mhcII_epitope_candidates_annotated.tsv b/docs/source/_static/test_mhcII_epitope_candidates_annotated.tsv index ccbf5551..5570e4b4 100644 --- a/docs/source/_static/test_mhcII_epitope_candidates_annotated.tsv +++ b/docs/source/_static/test_mhcII_epitope_candidates_annotated.tsv @@ -1,105 +1,84 @@ -position mutatedPeptide wildTypePeptide affinityMutated rankMutated affinityWildType rankWildType patientIdentifier gene rnaExpression imputedGeneExpression dnaVariantAlleleFrequency rnaVariantAlleleFrequency isoformMhcII.name isoformMhcII.alphaChain.fullName isoformMhcII.alphaChain.name isoformMhcII.alphaChain.gene isoformMhcII.alphaChain.group isoformMhcII.alphaChain.protein isoformMhcII.betaChain.fullName isoformMhcII.betaChain.name isoformMhcII.betaChain.gene isoformMhcII.betaChain.group isoformMhcII.betaChain.protein patient_identifier IEDB_Immunogenicity MixMHC2pred_rank Selfsimilarity amplitude dissimilarity_score hex_alignment_score mutation_not_found_in_proteome pathogen_similarity -5 AAAAAAAAALAAAAA AAAAAAAAAFAAAAA 775.74 0.4 778.08 0.8 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.36258 0.783 0.9822502911203502 2 1e-05 246 1 0 -6 AAAAAAAALAAAAAA AAAAAAAAFAAAAAA 812.22 0.39 812.51 1.61 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.36258 0.532 0.981263768075705 4.1282 3e-05 389 1 0 -7 AAAAAAALAAAAAAA AAAAAAAFAAAAAAA 817.84 0.34 831.29 0.97 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.36258 0.73 0.980933318805297 2.8529 0 322 1 0 -8 AAAAAALAAAAAAAA AAAAAAFAAAAAAAA 769.27 0.79 617.85 0.33 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.36258 0.633 0.981263768075705 0.41772 1e-05 281 1 0 -9 AAAAALAAAAAAAAA AAAAAFAAAAAAAAA 707.77 0.37 543.14 0.05 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.36258 0.555 0.98225029112035 0.13514 0 369 1 0 -10 AAAALAAAAAAAAAA AAAAFAAAAAAAAAA 667.61 0.1 505.19 0.01 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.36258 0.349 0.9838711693573455 0.1 0 346 1 0 -11 AAALAAAAAAAAAAA AAAFAAAAAAAAAAA 612.72 0.04 465.8 0.0 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.36095 0.575 0.9860888907728267 0 0 335 1 0 -12 AALAAAAAAAAAAAA AAFAAAAAAAAAAAA 703.69 0.18 560.78 0.04 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.39518 0.79 0.9888559259233813 0.22222 0 336 1 0 -13 ALAAAAAAAAAAAAA AFAAAAAAAAAAAAA 824.45 0.56 678.63 0.28 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.41148 1.24 0.9921226615333941 0.5 0 341 1 0 -5 AAAAAAAAALAAAAA AAAAAAAAAFAAAAA 633.73 7.18 491.05 6.89 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.36258 0.783 0.9822502911203502 0.95961 1e-05 246 1 0 -6 AAAAAAAALAAAAAA AAAAAAAAFAAAAAA 653.53 5.99 547.35 6.37 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.36258 0.532 0.981263768075705 1.0634 3e-05 389 1 0 -7 AAAAAAALAAAAAAA AAAAAAAFAAAAAAA 694.34 5.77 574.09 4.43 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.36258 0.73 0.980933318805297 0.76776 0 322 1 0 -9 AAAAALAAAAAAAAA AAAAAFAAAAAAAAA 584.81 3.23 467.56 1.3 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.36258 0.555 0.98225029112035 0.40248 0 369 1 0 -10 AAAALAAAAAAAAAA AAAAFAAAAAAAAAA 524.6 1.23 443.41 0.67 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.36258 0.349 0.9838711693573455 0.54472 0 346 1 0 -11 AAALAAAAAAAAAAA AAAFAAAAAAAAAAA 495.4 0.78 452.64 0.47 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.36095 0.575 0.9860888907728267 0.60256 0 335 1 0 -12 AALAAAAAAAAAAAA AAFAAAAAAAAAAAA 593.48 2.6 553.29 1.57 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.39518 0.79 0.9888559259233813 0.60385 0 336 1 0 -13 ALAAAAAAAAAAAAA AFAAAAAAAAAAAAA 756.49 7.57 713.32 5.31 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.41148 1.24 0.9921226615333941 0.70145 0 341 1 0 -9 AAAAALAAAAAAAAA AAAAAFAAAAAAAAA 278.25 8.94 259.76 11.67 0.0 0.0 0.0 0.0 HLA-DRB1*04:04 HLA-DRB1*04:04 HLA-DRB1*04:04 DRB1 04 04 Ptx 0.36258 0.555 0.98225029112035 1.3054 0 369 1 0 -10 AAAALAAAAAAAAAA AAAAFAAAAAAAAAA 179.85 4.6 199.63 7.28 0.0 0.0 0.0 0.0 HLA-DRB1*04:04 HLA-DRB1*04:04 HLA-DRB1*04:04 DRB1 04 04 Ptx 0.36258 0.349 0.9838711693573455 1.5826 0 346 1 0 -11 AAALAAAAAAAAAAA AAAFAAAAAAAAAAA 142.15 3.57 169.07 5.85 0.0 0.0 0.0 0.0 HLA-DRB1*04:04 HLA-DRB1*04:04 HLA-DRB1*04:04 DRB1 04 04 Ptx 0.36095 0.575 0.9860888907728267 1.6387 0 335 1 0 -12 AALAAAAAAAAAAAA AAFAAAAAAAAAAAA 193.07 9.05 213.1 13.85 0.0 0.0 0.0 0.0 HLA-DRB1*04:04 HLA-DRB1*04:04 HLA-DRB1*04:04 DRB1 04 04 Ptx 0.39518 0.79 0.9888559259233813 1.5304 0 336 1 0 -11 AAALAAAAAAAAAAA AAAFAAAAAAAAAAA 584.1 7.6 535.79 9.79 0.0 0.0 0.0 0.0 HLA-DRB1*15:01 HLA-DRB1*15:01 HLA-DRB1*15:01 DRB1 15 01 Ptx 0.36095 0.575 0.9860888907728267 1.2882 0 335 1 0 -5 AAAAAAAAALAAAAA AAAAAAAAAFAAAAA 15.77 0.03 20.72 0.27 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.36258 0.783 0.9822502911203502 9 1e-05 246 1 0 -6 AAAAAAAALAAAAAA AAAAAAAAFAAAAAA 15.65 0.04 22.47 0.41 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.36258 0.532 0.981263768075705 10.25 3e-05 389 1 0 -7 AAAAAAALAAAAAAA AAAAAAAFAAAAAAA 17.14 0.07 23.89 0.35 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.36258 0.73 0.980933318805297 5 0 322 1 0 -8 AAAAAALAAAAAAAA AAAAAAFAAAAAAAA 21.13 0.39 27.61 0.4 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.36258 0.633 0.981263768075705 1.0256 1e-05 281 1 0 -9 AAAAALAAAAAAAAA AAAAAFAAAAAAAAA 22.5 0.33 29.02 0.29 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.36258 0.555 0.98225029112035 0.87879 0 369 1 0 -10 AAAALAAAAAAAAAA AAAAFAAAAAAAAAA 22.35 0.13 27.86 0.22 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.36258 0.349 0.9838711693573455 1.6923 0 346 1 0 -11 AAALAAAAAAAAAAA AAAFAAAAAAAAAAA 21.21 0.07 26.86 0.11 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.36095 0.575 0.9860888907728267 1.5714 0 335 1 0 -12 AALAAAAAAAAAAAA AAFAAAAAAAAAAAA 23.24 0.08 27.69 0.08 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.39518 0.79 0.9888559259233813 1 0 336 1 0 -13 ALAAAAAAAAAAAAA AFAAAAAAAAAAAAA 28.07 0.1 29.39 0.09 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.41148 1.24 0.9921226615333941 0.9 0 341 1 0 -5 AAAAAAAAALAAAAA AAAAAAAAAFAAAAA 3415.63 2.93 2585.77 2.12 0.0 0.0 0.0 0.0 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 Ptx 0.36258 0.783 0.9822502911203502 0.72355 1e-05 246 1 0 -6 AAAAAAAALAAAAAA AAAAAAAAFAAAAAA 2958.8 1.55 2154.78 0.99 0.0 0.0 0.0 0.0 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 Ptx 0.36258 0.532 0.981263768075705 0.63871 3e-05 389 1 0 -7 AAAAAAALAAAAAAA AAAAAAAFAAAAAAA 2710.11 1.53 2144.94 1.06 0.0 0.0 0.0 0.0 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 Ptx 0.36258 0.73 0.980933318805297 0.69281 0 322 1 0 -8 AAAAAALAAAAAAAA AAAAAAFAAAAAAAA 2459.74 3.18 1299.57 0.73 0.0 0.0 0.0 0.0 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 Ptx 0.36258 0.633 0.981263768075705 0.22956 1e-05 281 1 0 -9 AAAAALAAAAAAAAA AAAAAFAAAAAAAAA 1990.01 1.81 940.81 0.32 0.0 0.0 0.0 0.0 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 Ptx 0.36258 0.555 0.98225029112035 0.1768 0 369 1 0 -10 AAAALAAAAAAAAAA AAAAFAAAAAAAAAA 1561.25 0.83 698.42 0.16 0.0 0.0 0.0 0.0 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 Ptx 0.36258 0.349 0.9838711693573455 0.19277 0 346 1 0 -11 AAALAAAAAAAAAAA AAAFAAAAAAAAAAA 1308.41 0.62 542.44 0.12 0.0 0.0 0.0 0.0 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 Ptx 0.36095 0.575 0.9860888907728267 0.19355 0 335 1 0 -12 AALAAAAAAAAAAAA AAFAAAAAAAAAAAA 1572.88 1.72 671.31 0.5 0.0 0.0 0.0 0.0 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 Ptx 0.39518 0.79 0.9888559259233813 0.2907 0 336 1 0 -13 ALAAAAAAAAAAAAA AFAAAAAAAAAAAAA 2211.13 4.46 929.37 2.68 0.0 0.0 0.0 0.0 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 Ptx 0.41148 1.24 0.9921226615333941 0.6009 0 341 1 0 -5 AAAAAAAAALAAAAA AAAAAAAAAFAAAAA 988.63 4.52 658.16 3.67 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.36258 0.783 0.9822502911203502 0.81195 1e-05 246 1 0 -6 AAAAAAAALAAAAAA AAAAAAAAFAAAAAA 1040.53 4.39 702.02 2.95 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.36258 0.532 0.981263768075705 0.67198 3e-05 389 1 0 -7 AAAAAAALAAAAAAA AAAAAAAFAAAAAAA 998.08 3.75 717.94 2.67 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.36258 0.73 0.980933318805297 0.712 0 322 1 0 -8 AAAAAALAAAAAAAA AAAAAAFAAAAAAAA 1075.48 5.69 695.28 1.57 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.36258 0.633 0.981263768075705 0.27592 1e-05 281 1 0 -9 AAAAALAAAAAAAAA AAAAAFAAAAAAAAA 1116.09 5.4 744.22 1.03 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.36258 0.555 0.98225029112035 0.19074 0 369 1 0 -10 AAAALAAAAAAAAAA AAAAFAAAAAAAAAA 1119.5 4.0 768.85 0.61 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.36258 0.349 0.9838711693573455 0.1525 0 346 1 0 -11 AAALAAAAAAAAAAA AAAFAAAAAAAAAAA 1106.38 2.76 834.87 0.37 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.36095 0.575 0.9860888907728267 0.13406 0 335 1 0 -12 AALAAAAAAAAAAAA AAFAAAAAAAAAAAA 1248.22 4.25 1005.79 1.58 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.39518 0.79 0.9888559259233813 0.37176 0 336 1 0 -13 ALAAAAAAAAAAAAA AFAAAAAAAAAAAAA 1435.11 5.84 1159.82 3.96 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.41148 1.24 0.9921226615333941 0.67808 0 341 1 0 -1 AAAAAAAAAAAAARA AAAAAAAAAAAAAMA 1511.84 2.77 1323.48 4.81 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.41886 1.52 0.9886461245535874 1.7365 0 349 1 0 -2 AAAAAAAAAAAARAA AAAAAAAAAAAAMAA 1355.29 2.24 1185.64 4.1 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.42214 2.28 0.9840173345571813 1.8304 0 369 1 0 -3 AAAAAAAAAAARAAA AAAAAAAAAAAMAAA 1458.83 2.75 1103.83 4.27 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.42337 1.65 0.9801341662894537 1.5527 0 339 1 0 -4 AAAAAAAAAARAAAA AAAAAAAAAAMAAAA 1244.72 2.32 970.05 4.64 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.42378 2.26 0.9770469333485702 2 0 336 1 0 -5 AAAAAAAAARAAAAA AAAAAAAAAMAAAAA 1313.38 2.71 947.08 4.03 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.42378 2.32 0.9748046960972773 1.4871 0.00382 289 1 0 -6 AAAAAAAARAAAAAA AAAAAAAAMAAAAAA 1483.05 3.03 917.88 4.47 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.42378 1.85 0.9734459201423025 1.4752 0.99994 239 1 0 -7 AAAAAAARAAAAAAA AAAAAAAMAAAAAAA 1461.69 2.32 915.62 5.39 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.42378 2.14 0.9729918667892427 2.3233 1e-05 409 1 0 -8 AAAAAARAAAAAAAA AAAAAAMAAAAAAAA 1508.36 3.04 950.08 4.72 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.42378 0.15 0.9734459201423027 1.5526 1e-05 226 1 0 -9 AAAAARAAAAAAAAA AAAAAMAAAAAAAAA 1466.44 2.71 907.21 3.1 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.42378 0.0473 0.9748046960972773 1.1439 1e-05 284 1 0 -10 AAAARAAAAAAAAAA AAAAMAAAAAAAAAA 1395.27 2.26 897.36 1.96 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.42378 0.0176 0.9770469333485704 0.86726 0.99239 346 1 0 -11 AAARAAAAAAAAAAA AAAMAAAAAAAAAAA 1464.32 2.77 995.3 1.78 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.42419 0.048 0.9801341662894535 0.6426 0 343 1 0 -1 AAAAAAAAAAAAARA AAAAAAAAAAAAAMA 5840.63 2.39 5798.69 6.28 0.0 0.0 0.0 0.0 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 Ptx 0.41886 1.52 0.9886461245535874 2.6276 0 349 1 0 -2 AAAAAAAAAAAARAA AAAAAAAAAAAAMAA 5167.9 1.5 4819.91 4.45 0.0 0.0 0.0 0.0 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 Ptx 0.42214 2.28 0.9840173345571813 2.9667 0 369 1 0 -3 AAAAAAAAAAARAAA AAAAAAAAAAAMAAA 4422.07 1.26 3998.71 3.26 0.0 0.0 0.0 0.0 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 Ptx 0.42337 1.65 0.9801341662894537 2.5873 0 339 1 0 -4 AAAAAAAAAARAAAA AAAAAAAAAAMAAAA 4015.1 1.59 3412.01 3.32 0.0 0.0 0.0 0.0 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 Ptx 0.42378 2.26 0.9770469333485702 2.088 0 336 1 0 -5 AAAAAAAAARAAAAA AAAAAAAAAMAAAAA 4553.8 2.74 3508.29 4.28 0.0 0.0 0.0 0.0 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 Ptx 0.42378 2.32 0.9748046960972773 1.562 0.00382 289 1 0 -6 AAAAAAAARAAAAAA AAAAAAAAMAAAAAA 4749.3 1.95 3495.48 3.68 0.0 0.0 0.0 0.0 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 Ptx 0.42378 1.85 0.9734459201423025 1.8872 0.99994 239 1 0 -7 AAAAAAARAAAAAAA AAAAAAAMAAAAAAA 3586.24 0.44 3424.69 2.93 0.0 0.0 0.0 0.0 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 Ptx 0.42378 2.14 0.9729918667892427 6.6591 1e-05 409 1 0 -8 AAAAAARAAAAAAAA AAAAAAMAAAAAAAA 668.32 0.0 3016.89 3.7 0.0 0.0 0.0 0.0 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 Ptx 0.42378 0.15 0.9734459201423027 NA 1e-05 226 1 0 -9 AAAAARAAAAAAAAA AAAAAMAAAAAAAAA 394.13 0.0 2471.8 2.33 0.0 0.0 0.0 0.0 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 Ptx 0.42378 0.0473 0.9748046960972773 NA 1e-05 284 1 0 -10 AAAARAAAAAAAAAA AAAAMAAAAAAAAAA 296.13 0.0 2096.25 1.31 0.0 0.0 0.0 0.0 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 Ptx 0.42378 0.0176 0.9770469333485704 NA 0.99239 346 1 0 -11 AAARAAAAAAAAAAA AAAMAAAAAAAAAAA 264.65 0.0 1835.64 1.03 0.0 0.0 0.0 0.0 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 Ptx 0.42419 0.048 0.9801341662894535 NA 0 343 1 0 -1 AAAAAAAAAAAAARA AAAAAAAAAAAAAMA 34.83 0.03 33.35 0.09 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.41886 1.52 0.9886461245535874 3 0 349 1 0 -2 AAAAAAAAAAAARAA AAAAAAAAAAAAMAA 30.04 0.03 26.8 0.09 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.42214 2.28 0.9840173345571813 3 0 369 1 0 -3 AAAAAAAAAAARAAA AAAAAAAAAAAMAAA 28.35 0.03 19.73 0.15 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.42337 1.65 0.9801341662894537 5 0 339 1 0 -4 AAAAAAAAAARAAAA AAAAAAAAAAMAAAA 23.91 0.01 15.95 0.06 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.42378 2.26 0.9770469333485702 6 0 336 1 0 -5 AAAAAAAAARAAAAA AAAAAAAAAMAAAAA 21.67 0.01 14.36 0.05 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.42378 2.32 0.9748046960972773 5 0.00382 289 1 0 -6 AAAAAAAARAAAAAA AAAAAAAAMAAAAAA 22.36 0.02 14.69 0.05 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.42378 1.85 0.9734459201423025 2.5 0.99994 239 1 0 -7 AAAAAAARAAAAAAA AAAAAAAMAAAAAAA 22.74 0.03 16.47 0.07 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.42378 2.14 0.9729918667892427 2.3333 1e-05 409 1 0 -8 AAAAAARAAAAAAAA AAAAAAMAAAAAAAA 27.93 0.06 17.78 0.27 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.42378 0.15 0.9734459201423027 4.5 1e-05 226 1 0 -9 AAAAARAAAAAAAAA AAAAAMAAAAAAAAA 30.43 0.07 18.01 0.13 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.42378 0.0473 0.9748046960972773 1.8571 1e-05 284 1 0 -10 AAAARAAAAAAAAAA AAAAMAAAAAAAAAA 32.5 0.05 17.62 0.06 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.42378 0.0176 0.9770469333485704 1.2 0.99239 346 1 0 -11 AAARAAAAAAAAAAA AAAMAAAAAAAAAAA 34.6 0.05 17.16 0.04 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.42419 0.048 0.9801341662894535 0.8 0 343 1 0 -1 AAAAAAAAAAAAARA AAAAAAAAAAAAAMA 851.97 0.14 1053.73 0.64 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.41886 1.52 0.9886461245535874 4.5714 0 349 1 0 -2 AAAAAAAAAAAARAA AAAAAAAAAAAAMAA 677.62 0.13 898.47 0.58 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.42214 2.28 0.9840173345571813 4.4615 0 369 1 0 -3 AAAAAAAAAAARAAA AAAAAAAAAAAMAAA 622.85 0.14 752.33 0.82 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.42337 1.65 0.9801341662894537 5.8571 0 339 1 0 -4 AAAAAAAAAARAAAA AAAAAAAAAAMAAAA 516.75 0.08 691.14 0.46 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.42378 2.26 0.9770469333485702 5.75 0 336 1 0 -5 AAAAAAAAARAAAAA AAAAAAAAAMAAAAA 482.08 0.07 689.4 0.35 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.42378 2.32 0.9748046960972773 5 0.00382 289 1 0 -6 AAAAAAAARAAAAAA AAAAAAAAMAAAAAA 507.17 0.08 721.2 0.45 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.42378 1.85 0.9734459201423025 5.625 0.99994 239 1 0 -7 AAAAAAARAAAAAAA AAAAAAAMAAAAAAA 497.47 0.07 710.44 0.48 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.42378 2.14 0.9729918667892427 6.8571 1e-05 409 1 0 -8 AAAAAARAAAAAAAA AAAAAAMAAAAAAAA 528.6 0.13 628.26 0.83 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.42378 0.15 0.9734459201423027 6.3846 1e-05 226 1 0 -9 AAAAARAAAAAAAAA AAAAAMAAAAAAAAA 540.55 0.06 555.02 0.19 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.42378 0.0473 0.9748046960972773 3.1667 1e-05 284 1 0 -10 AAAARAAAAAAAAAA AAAAMAAAAAAAAAA 543.22 0.05 551.31 0.05 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.42378 0.0176 0.9770469333485704 1 0.99239 346 1 0 -11 AAARAAAAAAAAAAA AAAMAAAAAAAAAAA 631.3 0.11 527.33 0.03 0.0 0.0 0.0 0.0 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 Ptx 0.42419 0.048 0.9801341662894535 0.27273 0 343 1 0 -1 AAAAAAAAAAAAARA AAAAAAAAAAAAAMA 1037.44 3.94 860.3 5.49 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.41886 1.52 0.9886461245535874 1.3934 0 349 1 0 -2 AAAAAAAAAAAARAA AAAAAAAAAAAAMAA 923.33 3.37 703.91 4.81 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.42214 2.28 0.9840173345571813 1.4273 0 369 1 0 -3 AAAAAAAAAAARAAA AAAAAAAAAAAMAAA 933.47 3.57 621.56 4.73 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.42337 1.65 0.9801341662894537 1.3249 0 339 1 0 -4 AAAAAAAAAARAAAA AAAAAAAAAAMAAAA 784.97 2.47 591.88 5.99 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.42378 2.26 0.9770469333485702 2.4251 0 336 1 0 -5 AAAAAAAAARAAAAA AAAAAAAAAMAAAAA 791.86 3.32 626.93 9.48 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.42378 2.32 0.9748046960972773 2.8554 0.00382 289 1 0 -6 AAAAAAAARAAAAAA AAAAAAAAMAAAAAA 826.67 3.38 674.0 8.54 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.42378 1.85 0.9734459201423025 2.5266 0.99994 239 1 0 -7 AAAAAAARAAAAAAA AAAAAAAMAAAAAAA 793.58 2.06 661.79 5.5 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.42378 2.14 0.9729918667892427 2.6699 1e-05 409 1 0 -8 AAAAAARAAAAAAAA AAAAAAMAAAAAAAA 759.9 2.5 592.84 6.39 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.42378 0.15 0.9734459201423027 2.556 1e-05 226 1 0 -9 AAAAARAAAAAAAAA AAAAAMAAAAAAAAA 738.78 1.21 533.46 2.07 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.42378 0.0473 0.9748046960972773 1.7107 1e-05 284 1 0 -10 AAAARAAAAAAAAAA AAAAMAAAAAAAAAA 713.14 0.69 491.92 0.84 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.42378 0.0176 0.9770469333485704 1.2174 0.99239 346 1 0 -11 AAARAAAAAAAAAAA AAAMAAAAAAAAAAA 774.9 1.37 494.5 0.56 0.0 0.0 0.0 0.0 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 Ptx 0.42419 0.048 0.9801341662894535 0.40876 0 343 1 0 +position mutatedPeptide wildTypePeptide core affinityMutated rankMutated affinityWildType rankWildType patientIdentifier gene rnaExpression imputedGeneExpression dnaVariantAlleleFrequency rnaVariantAlleleFrequency isoformMhcII.name isoformMhcII.alphaChain.fullName isoformMhcII.alphaChain.name isoformMhcII.alphaChain.gene isoformMhcII.alphaChain.group isoformMhcII.alphaChain.protein isoformMhcII.betaChain.fullName isoformMhcII.betaChain.name isoformMhcII.betaChain.gene isoformMhcII.betaChain.group isoformMhcII.betaChain.protein mutatedXmer IEDB_Immunogenicity MixMHC2pred_rank Of Selfsimilarity amplitude coreRel dissimilarity_score hex_alignment_score mutation_not_found_in_proteome pathogen_similarity +10 AAAALAAAAAAAAAA AAAAFAAAAAAAAAA LAAAAAAAA 299.87 2.42 370.86 4.69 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DRB1*04:04 HLA-DRB1*04:04 HLA-DRB1*04:04 DRB1 04 04 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.36258 0.00636 4 0.9838711693573455 1.938 1.000 0 346 1 0 +11 AAALAAAAAAAAAAA AAAFAAAAAAAAAAA LAAAAAAAA 220.03 1.55 290.07 3.4 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DRB1*04:04 HLA-DRB1*04:04 HLA-DRB1*04:04 DRB1 04 04 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.36095 0.00636 3 0.9860888907728267 2.1936 1.000 0 335 1 0 +7 AAAAAAALAAAAAAA AAAAAAAFAAAAAAA AAAALAAAA 626.09 4.07 574.5 8.62 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.36258 0.00438 3 0.980933318805297 2.1179 0.710 0 322 1 0 +9 AAAAALAAAAAAAAA AAAAAFAAAAAAAAA LAAAAAAAA 448.93 4.3 412.12 1.86 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.36258 0.00636 5 0.98225029112035 0.43256 0.610 0 369 1 0 +10 AAAALAAAAAAAAAA AAAAFAAAAAAAAAA LAAAAAAAA 436.25 1.56 385.43 0.63 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.36258 0.00636 4 0.9838711693573455 0.40385 0.780 0 346 1 0 +11 AAALAAAAAAAAAAA AAAFAAAAAAAAAAA LAAAAAAAA 422.24 0.73 380.1 0.25 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.36095 0.00636 3 0.9860888907728267 0.34247 0.890 0 335 1 0 +12 AALAAAAAAAAAAAA AAFAAAAAAAAAAAA AAAAAAAAA 586.51 3.34 511.19 1.28 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.39518 0.00636 3 0.9888559259233813 0.38323 0.470 0 336 1 0 +13 ALAAAAAAAAAAAAA AFAAAAAAAAAAAAA AAAAAAAAA 869.49 4.63 745.22 4.59 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.41148 0.00636 3 0.9921226615333941 0.99136 0.700 0 341 1 0 +5 AAAAAAAAALAAAAA AAAAAAAAAFAAAAA AAAAAALAA 16.2 0.16 21.64 0.49 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.36258 0.00438 3 0.9822502911203502 3.0625 0.410 1e-05 246 1 0 +6 AAAAAAAALAAAAAA AAAAAAAAFAAAAAA AAAAALAAA 14.84 0.14 21.8 1.0 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.36258 0.00438 3 0.981263768075705 7.1429 0.740 3e-05 389 1 0 +7 AAAAAAALAAAAAAA AAAAAAAFAAAAAAA AAAALAAAA 15.9 0.25 22.49 0.84 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.36258 0.00438 3 0.980933318805297 3.36 0.680 0 322 1 0 +8 AAAAAALAAAAAAAA AAAAAAFAAAAAAAA AAAALAAAA 20.01 1.02 27.2 1.33 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.36258 0.00636 2 0.981263768075705 1.3039 0.640 1e-05 281 1 0 +9 AAAAALAAAAAAAAA AAAAAFAAAAAAAAA LAAAAAAAA 24.64 0.83 28.29 0.76 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.36258 0.00636 5 0.98225029112035 0.91566 0.390 0 369 1 0 +10 AAAALAAAAAAAAAA AAAAFAAAAAAAAAA LAAAAAAAA 26.67 0.31 31.2 0.58 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.36258 0.00636 4 0.9838711693573455 1.871 0.620 0 346 1 0 +11 AAALAAAAAAAAAAA AAAFAAAAAAAAAAA LAAAAAAAA 26.19 0.17 36.61 0.39 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.36095 0.00636 3 0.9860888907728267 2.2941 0.770 0 335 1 0 +12 AALAAAAAAAAAAAA AAFAAAAAAAAAAAA AAAAAAAAA 29.73 0.21 38.72 0.24 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.39518 0.00636 3 0.9888559259233813 1.1429 0.790 0 336 1 0 +13 ALAAAAAAAAAAAAA AFAAAAAAAAAAAAA AAAAAAAAA 35.5 0.25 43.98 0.25 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.41148 0.00636 3 0.9921226615333941 1 1.000 0 341 1 0 +7 AAAAAAALAAAAAAA AAAAAAAFAAAAAAA AAAALAAAA 1263.13 4.23 690.52 4.81 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.36258 0.00438 3 0.980933318805297 1.1371 0.890 0 322 1 0 +11 AAALAAAAAAAAAAA AAAFAAAAAAAAAAA AAAAAAAAA 1616.74 4.67 1205.31 3.34 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.36095 0.00636 4 0.9860888907728267 0.7152 0.520 0 335 1 0 +12 AALAAAAAAAAAAAA AAFAAAAAAAAAAAA AAAAAAAAA 1896.51 4.66 1528.72 3.83 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.39518 0.00636 3 0.9888559259233813 0.82189 0.790 0 336 1 0 +13 ALAAAAAAAAAAAAA AFAAAAAAAAAAAAA AAAAAAAAA 2155.31 4.66 1733.26 4.28 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.41148 0.00636 3 0.9921226615333941 0.91845 0.740 0 341 1 0 +5 AAAAAAAAALAAAAA AAAAAAAAAFAAAAA AAAAAALAA 1319.01 0.32 1237.75 0.34 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.36258 0.00438 3 0.9822502911203502 1.0625 0.430 1e-05 246 1 0 +6 AAAAAAAALAAAAAA AAAAAAAAFAAAAAA AAAALAAAA 1290.28 0.29 1206.96 0.56 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.36258 0.00438 4 0.981263768075705 1.931 0.490 3e-05 389 1 0 +7 AAAAAAALAAAAAAA AAAAAAAFAAAAAAA AAAALAAAA 1383.7 0.18 1193.61 0.44 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.36258 0.00438 3 0.980933318805297 2.4444 0.730 0 322 1 0 +8 AAAAAALAAAAAAAA AAAAAAFAAAAAAAA AAAALAAAA 1381.42 0.63 959.31 0.38 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.36258 0.00636 2 0.981263768075705 0.60317 0.440 1e-05 281 1 0 +9 AAAAALAAAAAAAAA AAAAAFAAAAAAAAA LAAAAAAAA 1481.58 0.28 949.04 0.04 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.36258 0.00636 5 0.98225029112035 0.14286 0.530 0 369 1 0 +10 AAAALAAAAAAAAAA AAAAFAAAAAAAAAA LAAAAAAAA 1529.98 0.08 916.93 0.01 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.36258 0.00636 4 0.9838711693573455 0.125 0.670 0 346 1 0 +11 AAALAAAAAAAAAAA AAAFAAAAAAAAAAA LAAAAAAAA 1556.24 0.03 1009.15 0.01 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.36095 0.00636 3 0.9860888907728267 0.33333 0.750 0 335 1 0 +12 AALAAAAAAAAAAAA AAFAAAAAAAAAAAA AAAAAAAAA 1691.67 0.11 1186.0 0.03 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.39518 0.00636 3 0.9888559259233813 0.27273 0.620 0 336 1 0 +13 ALAAAAAAAAAAAAA AFAAAAAAAAAAAAA AAAAAAAAA 1967.53 0.19 1456.81 0.14 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.41148 0.00636 3 0.9921226615333941 0.73684 0.830 0 341 1 0 +10 AAAALAAAAAAAAAA AAAAFAAAAAAAAAA LAAAAAAAA 1918.67 4.28 681.99 3.18 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.36258 0.00636 4 0.9838711693573455 0.74299 0.910 0 346 1 0 +11 AAALAAAAAAAAAAA AAAFAAAAAAAAAAA LAAAAAAAA 1342.38 2.94 490.86 2.02 Ptx BRCA2 0.519506894 0.5365996317 0.857 0.294 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 AAAAAAAAAAAAALAAAAAAAAAAAAA 0.36095 0.00636 3 0.9860888907728267 0.68707 1.000 0 335 1 0 +1 AAAAAAAAAAAAARA AAAAAAAAAAAAAMA AAAAAAAAA 1503.2 2.08 1407.83 4.28 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.41886 0.00636 3 0.9886461245535874 2.0577 0.890 0 349 1 0 +2 AAAAAAAAAAAARAA AAAAAAAAAAAAMAA AAAAAAAAA 1217.98 1.99 967.74 4.11 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42214 0.00438 3 0.9840173345571813 2.0653 0.780 0 369 1 0 +3 AAAAAAAAAAARAAA AAAAAAAAAAAMAAA AAAAAAARA 1185.45 2.27 798.77 7.82 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42337 0.00636 4 0.9801341662894537 3.4449 0.660 0 339 1 0 +4 AAAAAAAAAARAAAA AAAAAAAAAAMAAAA AAAAAAARA 949.24 1.3 727.38 7.0 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00636 3 0.9770469333485702 5.3846 0.750 0 336 1 0 +5 AAAAAAAAARAAAAA AAAAAAAAAMAAAAA AAAAAAARA 861.35 1.8 644.72 9.94 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00438 2 0.9748046960972773 5.5222 0.270 0.00382 289 1 0 +6 AAAAAAAARAAAAAA AAAAAAAAMAAAAAA AAAARAAAA 844.08 2.57 603.34 7.53 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00636 4 0.9734459201423025 2.93 0.750 0.99994 266 1 0 +7 AAAAAAARAAAAAAA AAAAAAAMAAAAAAA AAAARAAAA 763.88 1.45 607.3 4.44 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00438 3 0.9729918667892427 3.0621 0.840 0 409 1 0 +8 AAAAAARAAAAAAAA AAAAAAMAAAAAAAA AAAARAAAA 725.4 2.91 488.64 7.19 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00636 2 0.9734459201423027 2.4708 0.350 0 226 1 0 +9 AAAAARAAAAAAAAA AAAAAMAAAAAAAAA ARAAAAAAA 650.36 1.1 431.92 3.84 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00636 4 0.9748046960972773 3.4909 0.390 0 284 1 0 +10 AAAARAAAAAAAAAA AAAAMAAAAAAAAAA RAAAAAAAA 622.78 0.38 395.39 1.42 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00438 4 0.9770469333485704 3.7368 0.470 0 346 1 0 +11 AAARAAAAAAAAAAA AAAMAAAAAAAAAAA RAAAAAAAA 644.98 0.31 386.14 0.57 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*01:02-DQB1*03:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42419 0.00636 3 0.9801341662894535 1.8387 0.580 0 343 1 0 +1 AAAAAAAAAAAAARA AAAAAAAAAAAAAMA AAAAAAAAA 50.81 0.09 52.07 0.23 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.41886 0.00636 3 0.9886461245535874 2.5556 1.000 0 349 1 0 +2 AAAAAAAAAAAARAA AAAAAAAAAAAAMAA AAAAAAAAA 35.97 0.09 38.41 0.23 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42214 0.00438 3 0.9840173345571813 2.5556 0.910 0 369 1 0 +3 AAAAAAAAAAARAAA AAAAAAAAAAAMAAA AAAAAAARA 26.82 0.08 24.16 0.58 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42337 0.00636 4 0.9801341662894537 7.25 0.500 0 339 1 0 +4 AAAAAAAAAARAAAA AAAAAAAAAAMAAAA AAAAAARAA 19.22 0.02 17.29 0.29 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00636 4 0.9770469333485702 14.5 0.610 0 336 1 0 +5 AAAAAAAAARAAAAA AAAAAAAAAMAAAAA AAAAAARAA 14.95 0.01 14.82 0.21 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00438 3 0.9748046960972773 21 0.860 0.00382 289 1 0 +6 AAAAAAAARAAAAAA AAAAAAAAMAAAAAA AAAARAAAA 13.79 0.05 14.47 0.2 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00636 4 0.9734459201423025 4 0.510 0.99994 266 1 0 +7 AAAAAAARAAAAAAA AAAAAAAMAAAAAAA AAAARAAAA 13.55 0.05 15.81 0.29 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00438 3 0.9729918667892427 5.8 0.970 0 409 1 0 +8 AAAAAARAAAAAAAA AAAAAAMAAAAAAAA AAAARAAAA 18.11 0.18 18.79 0.91 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00636 2 0.9734459201423027 5.0556 0.690 0 226 1 0 +9 AAAAARAAAAAAAAA AAAAAMAAAAAAAAA ARAAAAAAA 23.85 0.18 21.89 0.47 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00636 4 0.9748046960972773 2.6111 0.790 0 284 1 0 +10 AAAARAAAAAAAAAA AAAAMAAAAAAAAAA ARAAAAAAA 33.14 0.11 23.2 0.18 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00438 3 0.9770469333485704 1.6364 0.950 0 346 1 0 +11 AAARAAAAAAAAAAA AAAMAAAAAAAAAAA AAAAAAAAA 48.23 0.18 23.55 0.11 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*01:02-DQB1*06:02 HLA-DQA1*01:02 HLA-DQA1*01:02 DQA1 01 02 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42419 0.00636 4 0.9801341662894535 0.61111 0.650 0 343 1 0 +1 AAAAAAAAAAAAARA AAAAAAAAAAAAAMA AAAAAAAAA 2053.86 2.43 1531.3 4.1 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.41886 0.00636 3 0.9886461245535874 1.6872 0.780 0 349 1 0 +2 AAAAAAAAAAAARAA AAAAAAAAAAAAMAA AAAAAAAAA 1697.33 2.18 1146.14 4.03 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42214 0.00438 3 0.9840173345571813 1.8486 0.700 0 369 1 0 +3 AAAAAAAAAAARAAA AAAAAAAAAAAMAAA AAAAAAARA 1778.82 2.71 1236.06 5.82 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42337 0.00636 4 0.9801341662894537 2.1476 0.580 0 339 1 0 +4 AAAAAAAAAARAAAA AAAAAAAAAAMAAAA AAAAAAARA 1289.86 2.37 1171.37 6.27 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00636 3 0.9770469333485702 2.6456 0.710 0 336 1 0 +5 AAAAAAAAARAAAAA AAAAAAAAAMAAAAA AAAAAARAA 1233.94 3.44 1180.92 8.36 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00438 3 0.9748046960972773 2.4302 0.350 0.00382 289 1 0 +6 AAAAAAAARAAAAAA AAAAAAAAMAAAAAA AAAARAAAA 1334.83 3.86 1210.33 7.01 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00636 4 0.9734459201423025 1.8161 0.760 0.99994 266 1 0 +7 AAAAAAARAAAAAAA AAAAAAAMAAAAAAA AAAARAAAA 1294.14 2.92 1182.81 5.36 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00438 3 0.9729918667892427 1.8356 0.800 0 409 1 0 +8 AAAAAARAAAAAAAA AAAAAAMAAAAAAAA ARAAAAAAA 1430.88 3.8 1113.34 7.35 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00636 5 0.9734459201423027 1.9342 0.400 0 226 1 0 +9 AAAAARAAAAAAAAA AAAAAMAAAAAAAAA ARAAAAAAA 1603.43 2.71 1175.78 6.38 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00636 4 0.9748046960972773 2.3542 0.580 0 284 1 0 +10 AAAARAAAAAAAAAA AAAAMAAAAAAAAAA ARAAAAAAA 1689.73 2.6 1233.56 4.85 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00438 3 0.9770469333485704 1.8654 0.570 0 346 1 0 +11 AAARAAAAAAAAAAA AAAMAAAAAAAAAAA AAAAAAAAA 1947.31 3.15 1402.26 4.33 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*03:01-DQB1*03:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*03:02 HLA-DQB1*03:02 DQB1 03 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42419 0.00636 4 0.9801341662894535 1.3746 0.640 0 343 1 0 +1 AAAAAAAAAAAAARA AAAAAAAAAAAAAMA AAAAAAAAA 2537.32 0.04 2621.06 0.18 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.41886 0.00636 3 0.9886461245535874 4.5 0.940 0 349 1 0 +2 AAAAAAAAAAAARAA AAAAAAAAAAAAMAA AAAAAAAAA 1811.76 0.04 1870.56 0.15 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42214 0.00438 3 0.9840173345571813 3.75 0.760 0 369 1 0 +3 AAAAAAAAAAARAAA AAAAAAAAAAAMAAA AAAAAAARA 1538.74 0.04 1565.49 0.27 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42337 0.00636 4 0.9801341662894537 6.75 0.520 0 339 1 0 +4 AAAAAAAAAARAAAA AAAAAAAAAAMAAAA AAAAAAARA 1182.96 0.01 1344.7 0.24 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00636 3 0.9770469333485702 24 0.580 0 336 1 0 +5 AAAAAAAAARAAAAA AAAAAAAAAMAAAAA AAAAAARAA 1000.18 0.02 1224.0 0.45 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00438 3 0.9748046960972773 22.5 0.670 0.00382 289 1 0 +6 AAAAAAAARAAAAAA AAAAAAAAMAAAAAA AAAARAAAA 1055.41 0.04 1186.39 0.46 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00636 4 0.9734459201423025 11.5 0.550 0.99994 266 1 0 +7 AAAAAAARAAAAAAA AAAAAAAMAAAAAAA AAAARAAAA 1058.83 0.04 1185.08 0.2 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00438 3 0.9729918667892427 5 0.860 0 409 1 0 +8 AAAAAARAAAAAAAA AAAAAAMAAAAAAAA AAAARAAAA 1155.12 0.14 1211.53 0.24 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00636 2 0.9734459201423027 1.7143 0.470 0 226 1 0 +9 AAAAARAAAAAAAAA AAAAAMAAAAAAAAA ARAAAAAAA 1360.92 0.06 1302.37 0.09 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00636 4 0.9748046960972773 1.5 0.630 0 284 1 0 +10 AAAARAAAAAAAAAA AAAAMAAAAAAAAAA ARAAAAAAA 1409.63 0.03 1382.42 0.04 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00438 3 0.9770469333485704 1.3333 0.790 0 346 1 0 +11 AAARAAAAAAAAAAA AAAMAAAAAAAAAAA AAAAAAAAA 1782.73 0.06 1447.02 0.02 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DQA1*03:01-DQB1*06:02 HLA-DQA1*03:01 HLA-DQA1*03:01 DQA1 03 01 HLA-DQB1*06:02 HLA-DQB1*06:02 DQB1 06 02 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42419 0.00636 4 0.9801341662894535 0.33333 0.510 0 343 1 0 +2 AAAAAAAAAAAARAA AAAAAAAAAAAAMAA RAAAAAAAA 4080.35 4.82 6734.89 13.15 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42214 0.00438 2 0.9840173345571813 2.7282 0.720 0 369 1 0 +3 AAAAAAAAAAARAAA AAAAAAAAAAAMAAA RAAAAAAAA 2919.11 2.27 5452.99 12.76 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42337 0.00636 3 0.9801341662894537 5.6212 0.970 0 339 1 0 +4 AAAAAAAAAARAAAA AAAAAAAAAAMAAAA RAAAAAAAA 3309.97 3.06 5678.37 12.11 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00636 4 0.9770469333485702 3.9575 0.900 0 336 1 0 +8 AAAAAARAAAAAAAA AAAAAAMAAAAAAAA RAAAAAAAA 837.34 1.15 5458.01 17.13 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00636 6 0.9734459201423027 14.896 0.990 0 226 1 0 +9 AAAAARAAAAAAAAA AAAAAMAAAAAAAAA RAAAAAAAA 290.55 0.09 3936.21 10.44 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00636 5 0.9748046960972773 116 1.000 0 284 1 0 +10 AAAARAAAAAAAAAA AAAAMAAAAAAAAAA RAAAAAAAA 172.39 0.02 2900.22 5.67 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42378 0.00438 4 0.9770469333485704 283.5 1.000 0 346 1 0 +11 AAARAAAAAAAAAAA AAAMAAAAAAAAAAA RAAAAAAAA 122.83 0.01 2280.5 3.9 Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 HLA-DPA1*01:03-DPB1*06:01 HLA-DPA1*01:03 HLA-DPA1*01:03 DPA1 01 03 HLA-DPB1*06:01 HLA-DPB1*06:01 DPB1 06 01 AAAAAAAAAAAAARAAAAAAAAAAAAA 0.42419 0.00636 3 0.9801341662894535 390 1.000 0 343 1 0 diff --git a/docs/source/_static/test_mhcI_epitope_candidates_annotated.tsv b/docs/source/_static/test_mhcI_epitope_candidates_annotated.tsv index 3f718ba5..958fc89b 100644 --- a/docs/source/_static/test_mhcI_epitope_candidates_annotated.tsv +++ b/docs/source/_static/test_mhcI_epitope_candidates_annotated.tsv @@ -1,2 +1,2 @@ -patient_identifier position mutatedPeptide wildTypePeptide affinityMutated rankMutated affinityWildType rankWildType patientIdentifier gene rnaExpression imputedGeneExpression dnaVariantAlleleFrequency rnaVariantAlleleFrequency alleleMhcI.fullName alleleMhcI.name alleleMhcI.gene alleleMhcI.group alleleMhcI.protein DAI IEDB_Immunogenicity Improved_Binder_MHCI MixMHCpred_affinity_score MixMHCpred_rank PRIME_affinity_score PRIME_rank Priority_score Selfsimilarity Selfsimilarity_conserved_binder Tcell_predictor_score amplitude anchor_mutated dissimilarity_score hex_alignment_score mutation_not_found_in_proteome number_of_mismatches pathogen_similarity position_mutation recognition_potential -Ptx 9.0 AAAAARAAA AAAAAMAAA 199.38 1.346 1697.4 4.347 0.0 0.0 0.0 0.0 HLA-B*07:02 HLA-B*07:02 B 07 02 1498 0.19477 1 0.51774 0.08 0.18732 0.06 0.32903 0.966307602001344 NA 0.5173265191848659 5.6409 0 1 132 1 1 0 6 0 +patientIdentifier gene rnaExpression imputedGeneExpression dnaVariantAlleleFrequency rnaVariantAlleleFrequency mutatedXmer position mutatedPeptide wildTypePeptide core affinityMutated rankMutated affinityWildType rankWildType alleleMhcI.fullName alleleMhcI.name alleleMhcI.gene alleleMhcI.group alleleMhcI.protein DAI Gl Gp IEDB_Immunogenicity Icore Improved_Binder_MHCI MixMHCpred_rank MixMHCpred_score Of PRIME_rank PRIME_score Priority_score_fromDNA Priority_score_fromRNA Priority_score_imputed_fromDNA Priority_score_imputed_fromRNA Selfsimilarity Selfsimilarity_conserved_binder amplitude anchor_mutated dissimilarity_score hex_alignment_score mutation_not_found_in_proteome number_of_mismatches pathogen_similarity position_mutation recognition_potential +Ptx BRCA2 0.715756594 0.5365996317 0.556 0.173 AAAAAAAAAAAAARAAAAAAAAAAAAA 9.0 AAAAARAAA AAAAAMAAA AAAAARAAA 199.38 1.346 1697.4 4.347 HLA-B*07:02 HLA-B*07:02 B 07 02 1498 0 0 0.19477 AAAAARAAA 1 0.11351 -0.05382 0 0.159 0.08563 0.32903 0.10238 0.26268 0.08173 0.966307602001344 NA 5.6409 0 1 132 1 1 0 6 0 diff --git a/docs/source/_static/test_neoantigen_candidates_annotated.json b/docs/source/_static/test_neoantigen_candidates_annotated.json index f7d7506f..08a585ad 100644 --- a/docs/source/_static/test_neoantigen_candidates_annotated.json +++ b/docs/source/_static/test_neoantigen_candidates_annotated.json @@ -1 +1 @@ -[{"patient_identifier":"Ptx","gene":"BRCA2","position":[14],"wild_type_xmer":"AAAAAAAAAAAAAFAAAAAAAAAAAAA","mutated_xmer":"AAAAAAAAAAAAALAAAAAAAAAAAAA","rna_expression":0.5195068939999999,"imputed_gene_expression":0.5365996317,"dna_variant_allele_frequency":0.857,"rna_variant_allele_frequency":null,"neofox_annotations":{"annotations":[{"name":"Best_rank_MHCI_score","value":"4.389"},{"name":"Best_rank_MHCI_score_epitope","value":"AAALAAAAA"},{"name":"Best_rank_MHCI_score_allele","value":"HLA-B*07:02"},{"name":"Best_rank_MHCI_score_WT","value":"5.068"},{"name":"Best_rank_MHCI_score_epitope_WT","value":"AAAFAAAAA"},{"name":"Best_affinity_MHCI_score","value":"1018.2"},{"name":"Best_affinity_MHCI_epitope","value":"AALAAAAAAA"},{"name":"Best_affinity_MHCI_allele","value":"HLA-A*02:01"},{"name":"Best_affinity_MHCI_score_WT","value":"8184.1"},{"name":"Best_affinity_MHCI_epitope_WT","value":"AAFAAAAAAA"},{"name":"Best_rank_MHCI_9mer_score","value":"4.389"},{"name":"Best_rank_MHCI_9mer_epitope","value":"AAALAAAAA"},{"name":"Best_rank_MHCI_9mer_allele","value":"HLA-B*07:02"},{"name":"Best_rank_MHCI_9mer_score_WT","value":"5.068"},{"name":"Best_rank_MHCI_9mer_epitope_WT","value":"AAAFAAAAA"},{"name":"Best_affinity_MHCI_9mer_score","value":"1534.8"},{"name":"Best_affinity_MHCI_9mer_allele","value":"HLA-C*03:04"},{"name":"Best_affinity_MHCI_9mer_epitope","value":"AAALAAAAA"},{"name":"Best_affinity_MHCI_9mer_score_WT","value":"1180.8"},{"name":"Best_affinity_MHCI_9mer_epitope_WT","value":"AAAFAAAAA"},{"name":"PHBR_I","value":"8.5743"},{"name":"Generator_rate_MHCI","value":"0"},{"name":"Generator_rate_CDN_MHCI","value":"0"},{"name":"Generator_rate_ADN_MHCI","value":"0"},{"name":"Best_affinity_MHCI_9mer_position_mutation","value":"4"},{"name":"Best_affinity_MHCI_9mer_anchor_mutated","value":"0"},{"name":"Best_rank_MHCII_score","value":"0.03"},{"name":"Best_rank_MHCII_score_epitope","value":"AAAAAAAAALAAAAA"},{"name":"Best_rank_MHCII_score_allele","value":"HLA-DQA1*01:02-DQB1*06:02"},{"name":"Best_rank_MHCII_score_WT","value":"0.27"},{"name":"Best_rank_MHCII_score_epitope_WT","value":"AAAAAAAAAFAAAAA"},{"name":"Best_affinity_MHCII_score","value":"15.65"},{"name":"Best_affinity_MHCII_epitope","value":"AAAAAAAALAAAAAA"},{"name":"Best_affinity_MHCII_allele","value":"HLA-DQA1*01:02-DQB1*06:02"},{"name":"Best_affinity_MHCII_score_WT","value":"22.47"},{"name":"Best_affinity_MHCII_epitope_WT","value":"AAAAAAAAFAAAAAA"},{"name":"PHBR_II","value":"NA"},{"name":"Generator_rate_MHCII","value":"25"},{"name":"Generator_rate_CDN_MHCII","value":"21"},{"name":"Generator_rate_ADN_MHCII","value":"4"},{"name":"MixMHCpred_best_peptide","value":"AAALAAAAA"},{"name":"MixMHCpred_best_score","value":"0.3862"},{"name":"MixMHCpred_best_rank","value":"0.3"},{"name":"MixMHCpred_best_allele","value":"HLA-B*07:02"},{"name":"PRIME_best_peptide","value":"AAALAAAAA"},{"name":"PRIME_best_score","value":"0.18933"},{"name":"PRIME_best_rank","value":"0.04"},{"name":"PRIME_best_allele","value":"HLA-B*07:02"},{"name":"MixMHC2pred_best_peptide","value":"AAAALAAAAAAAAAAA"},{"name":"MixMHC2pred_best_rank","value":"0.296"},{"name":"MixMHC2pred_best_allele","value":"HLA-DPA1*01:03-DPB1*06:01"},{"name":"Expression_mutated_transcript","value":"0.44522"},{"name":"mutation_not_found_in_proteome","value":"1"},{"name":"Amplitude_MHCI_affinity_9mer","value":"0.56811"},{"name":"Amplitude_MHCI_affinity","value":"2.3262"},{"name":"Amplitude_MHCII_rank","value":"9"},{"name":"Pathogensimiliarity_MHCI_9mer","value":"0"},{"name":"Recognition_Potential_MHCI_9mer","value":"0"},{"name":"Pathogensimiliarity_MHCII","value":"0"},{"name":"DAI_MHCI_affinity","value":"7165.9"},{"name":"CDN_MHCI","value":"0"},{"name":"ADN_MHCI","value":"0"},{"name":"CDN_MHCII","value":"1"},{"name":"ADN_MHCII","value":"1"},{"name":"Tcell_predictor_score","value":"0.39121828442992074"},{"name":"Improved_Binder_MHCI","value":"0"},{"name":"Selfsimilarity_MHCII","value":"0.981263768075705"},{"name":"Selfsimilarity_MHCI","value":"0.9763465205057597"},{"name":"Selfsimilarity_MHCI_conserved_binder","value":"0.9763465205057597"},{"name":"Number_of_mismatches_MCHI","value":"1"},{"name":"Priority_score","value":"0"},{"name":"Neoag_immunogenicity","value":"13.16998"},{"name":"IEDB_Immunogenicity_MHCI","value":"0.18182"},{"name":"IEDB_Immunogenicity_MHCII","value":"0.36258"},{"name":"Dissimilarity_MHCI","value":"1"},{"name":"Dissimilarity_MHCII","value":"3e-05"},{"name":"vaxrank_binding_score","value":"0.01301"},{"name":"vaxrank_total_score","value":"0.00579"},{"name":"Hex_alignment_score_MHCI","value":"177"},{"name":"Hex_alignment_score_MHCII","value":"389"}],"annotator":"NeoFox","annotator_version":"0.7.0dev13","timestamp":"20220803154540091490","resources":[{"name":"netMHCpan","version":"4.1"},{"name":"netMHCIIpan","version":"4.0"},{"name":"mixMHCpred","version":"2.1"},{"name":"mixMHC2pred","version":"1.2"},{"name":"IEDB","url":"http://www.iedb.org/downloader.php?file_name=doc/tcell_full_v3.zip","hash":"09f325faadbe664b52d2bf0c1dd2b043","download_timestamp":"20220404152052"},{"name":"IMGT/HLA 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"wild_type_peptide": "AAAAAAAAAMAAAAA", "isoform_mhc_i_i": {"name": "HLA-DQA1*03:01-DQB1*06:02", "alpha_chain": {"full_name": "HLA-DQA1*03:01", "name": "HLA-DQA1*03:01", "gene": "DQA1", "group": "03", "protein": "01"}, "beta_chain": {"full_name": "HLA-DQB1*06:02", "name": "HLA-DQB1*06:02", "gene": "DQB1", "group": "06", "protein": "02"}}, "core": "AAAAAARAA", "affinity_mutated": 1000.18, "rank_mutated": 0.02, "affinity_wild_type": 1224.0, "rank_wild_type": 0.45}, {"position": 6, "mutated_peptide": "AAAAAAAARAAAAAA", "wild_type_peptide": "AAAAAAAAMAAAAAA", "isoform_mhc_i_i": {"name": "HLA-DQA1*03:01-DQB1*06:02", "alpha_chain": {"full_name": "HLA-DQA1*03:01", "name": "HLA-DQA1*03:01", "gene": "DQA1", "group": "03", "protein": "01"}, "beta_chain": {"full_name": "HLA-DQB1*06:02", "name": "HLA-DQB1*06:02", "gene": "DQB1", "group": "06", "protein": "02"}}, "core": "AAAARAAAA", "affinity_mutated": 1055.41, "rank_mutated": 0.04, "affinity_wild_type": 1186.39, "rank_wild_type": 0.46}, {"position": 7, "mutated_peptide": "AAAAAAARAAAAAAA", "wild_type_peptide": "AAAAAAAMAAAAAAA", "isoform_mhc_i_i": {"name": "HLA-DQA1*03:01-DQB1*06:02", "alpha_chain": {"full_name": "HLA-DQA1*03:01", "name": "HLA-DQA1*03:01", "gene": "DQA1", "group": "03", "protein": "01"}, "beta_chain": {"full_name": "HLA-DQB1*06:02", "name": "HLA-DQB1*06:02", "gene": "DQB1", "group": "06", "protein": "02"}}, "core": "AAAARAAAA", "affinity_mutated": 1058.83, "rank_mutated": 0.04, "affinity_wild_type": 1185.08, "rank_wild_type": 0.2}, {"position": 8, "mutated_peptide": "AAAAAARAAAAAAAA", "wild_type_peptide": "AAAAAAMAAAAAAAA", "isoform_mhc_i_i": {"name": "HLA-DQA1*03:01-DQB1*06:02", "alpha_chain": {"full_name": "HLA-DQA1*03:01", "name": "HLA-DQA1*03:01", "gene": "DQA1", "group": "03", "protein": "01"}, "beta_chain": {"full_name": "HLA-DQB1*06:02", "name": "HLA-DQB1*06:02", "gene": "DQB1", "group": "06", "protein": "02"}}, "core": "AAAARAAAA", "affinity_mutated": 1155.12, "rank_mutated": 0.14, "affinity_wild_type": 1211.53, "rank_wild_type": 0.24}, {"position": 9, "mutated_peptide": "AAAAARAAAAAAAAA", "wild_type_peptide": "AAAAAMAAAAAAAAA", "isoform_mhc_i_i": {"name": "HLA-DQA1*03:01-DQB1*06:02", "alpha_chain": {"full_name": "HLA-DQA1*03:01", "name": "HLA-DQA1*03:01", "gene": "DQA1", "group": "03", "protein": "01"}, "beta_chain": {"full_name": "HLA-DQB1*06:02", "name": "HLA-DQB1*06:02", "gene": "DQB1", "group": "06", "protein": "02"}}, "core": "ARAAAAAAA", "affinity_mutated": 1360.92, "rank_mutated": 0.06, "affinity_wild_type": 1302.37, "rank_wild_type": 0.09}, {"position": 10, "mutated_peptide": "AAAARAAAAAAAAAA", "wild_type_peptide": "AAAAMAAAAAAAAAA", "isoform_mhc_i_i": {"name": "HLA-DQA1*03:01-DQB1*06:02", "alpha_chain": {"full_name": "HLA-DQA1*03:01", "name": "HLA-DQA1*03:01", "gene": "DQA1", "group": "03", "protein": "01"}, "beta_chain": {"full_name": "HLA-DQB1*06:02", "name": "HLA-DQB1*06:02", "gene": "DQB1", "group": "06", "protein": "02"}}, "core": "ARAAAAAAA", "affinity_mutated": 1409.63, "rank_mutated": 0.03, "affinity_wild_type": 1382.42, "rank_wild_type": 0.04}, {"position": 11, "mutated_peptide": "AAARAAAAAAAAAAA", "wild_type_peptide": "AAAMAAAAAAAAAAA", "isoform_mhc_i_i": {"name": "HLA-DQA1*03:01-DQB1*06:02", "alpha_chain": {"full_name": "HLA-DQA1*03:01", "name": "HLA-DQA1*03:01", "gene": "DQA1", "group": "03", "protein": "01"}, "beta_chain": {"full_name": "HLA-DQB1*06:02", "name": "HLA-DQB1*06:02", "gene": "DQB1", "group": "06", "protein": "02"}}, "core": "AAAAAAAAA", "affinity_mutated": 1782.73, "rank_mutated": 0.06, "affinity_wild_type": 1447.02, "rank_wild_type": 0.02}, {"position": 2, "mutated_peptide": "AAAAAAAAAAAARAA", "wild_type_peptide": "AAAAAAAAAAAAMAA", "isoform_mhc_i_i": {"name": "HLA-DPA1*01:03-DPB1*06:01", "alpha_chain": {"full_name": "HLA-DPA1*01:03", "name": "HLA-DPA1*01:03", "gene": "DPA1", "group": "01", "protein": "03"}, "beta_chain": {"full_name": "HLA-DPB1*06:01", "name": "HLA-DPB1*06:01", "gene": "DPB1", "group": "06", "protein": "01"}}, "core": "RAAAAAAAA", "affinity_mutated": 4080.35, "rank_mutated": 4.82, "affinity_wild_type": 6734.89, "rank_wild_type": 13.15}, {"position": 3, "mutated_peptide": "AAAAAAAAAAARAAA", "wild_type_peptide": "AAAAAAAAAAAMAAA", "isoform_mhc_i_i": {"name": "HLA-DPA1*01:03-DPB1*06:01", "alpha_chain": {"full_name": "HLA-DPA1*01:03", "name": "HLA-DPA1*01:03", "gene": "DPA1", "group": "01", "protein": "03"}, "beta_chain": {"full_name": "HLA-DPB1*06:01", "name": "HLA-DPB1*06:01", "gene": "DPB1", "group": "06", "protein": "01"}}, "core": "RAAAAAAAA", "affinity_mutated": 2919.11, "rank_mutated": 2.27, "affinity_wild_type": 5452.99, "rank_wild_type": 12.76}, {"position": 4, "mutated_peptide": "AAAAAAAAAARAAAA", "wild_type_peptide": "AAAAAAAAAAMAAAA", "isoform_mhc_i_i": {"name": "HLA-DPA1*01:03-DPB1*06:01", "alpha_chain": {"full_name": "HLA-DPA1*01:03", "name": "HLA-DPA1*01:03", "gene": "DPA1", "group": "01", "protein": "03"}, "beta_chain": {"full_name": "HLA-DPB1*06:01", "name": "HLA-DPB1*06:01", "gene": "DPB1", "group": "06", "protein": "01"}}, "core": "RAAAAAAAA", "affinity_mutated": 3309.97, "rank_mutated": 3.06, "affinity_wild_type": 5678.37, "rank_wild_type": 12.11}, {"position": 8, "mutated_peptide": "AAAAAARAAAAAAAA", "wild_type_peptide": "AAAAAAMAAAAAAAA", "isoform_mhc_i_i": {"name": "HLA-DPA1*01:03-DPB1*06:01", "alpha_chain": {"full_name": "HLA-DPA1*01:03", "name": "HLA-DPA1*01:03", "gene": "DPA1", "group": "01", "protein": "03"}, "beta_chain": {"full_name": "HLA-DPB1*06:01", "name": "HLA-DPB1*06:01", "gene": "DPB1", "group": "06", "protein": "01"}}, "core": "RAAAAAAAA", "affinity_mutated": 837.34, "rank_mutated": 1.15, "affinity_wild_type": 5458.01, "rank_wild_type": 17.13}, {"position": 9, "mutated_peptide": "AAAAARAAAAAAAAA", "wild_type_peptide": "AAAAAMAAAAAAAAA", "isoform_mhc_i_i": {"name": "HLA-DPA1*01:03-DPB1*06:01", "alpha_chain": {"full_name": "HLA-DPA1*01:03", "name": "HLA-DPA1*01:03", "gene": "DPA1", "group": "01", "protein": "03"}, "beta_chain": {"full_name": "HLA-DPB1*06:01", "name": "HLA-DPB1*06:01", "gene": "DPB1", "group": "06", "protein": "01"}}, "core": "RAAAAAAAA", "affinity_mutated": 290.55, "rank_mutated": 0.09, "affinity_wild_type": 3936.21, "rank_wild_type": 10.44}, {"position": 10, "mutated_peptide": "AAAARAAAAAAAAAA", "wild_type_peptide": "AAAAMAAAAAAAAAA", "isoform_mhc_i_i": {"name": "HLA-DPA1*01:03-DPB1*06:01", "alpha_chain": {"full_name": "HLA-DPA1*01:03", "name": "HLA-DPA1*01:03", "gene": "DPA1", "group": "01", "protein": "03"}, "beta_chain": {"full_name": "HLA-DPB1*06:01", "name": "HLA-DPB1*06:01", "gene": "DPB1", "group": "06", "protein": "01"}}, "core": "RAAAAAAAA", "affinity_mutated": 172.39, "rank_mutated": 0.02, "affinity_wild_type": 2900.22, "rank_wild_type": 5.67}, {"position": 11, "mutated_peptide": "AAARAAAAAAAAAAA", "wild_type_peptide": "AAAMAAAAAAAAAAA", "isoform_mhc_i_i": {"name": "HLA-DPA1*01:03-DPB1*06:01", "alpha_chain": {"full_name": "HLA-DPA1*01:03", "name": "HLA-DPA1*01:03", "gene": "DPA1", "group": "01", "protein": "03"}, "beta_chain": {"full_name": "HLA-DPB1*06:01", "name": "HLA-DPB1*06:01", "gene": "DPB1", "group": "06", "protein": "01"}}, "core": "RAAAAAAAA", "affinity_mutated": 122.83, "rank_mutated": 0.01, "affinity_wild_type": 2280.5, "rank_wild_type": 3.9}]}] \ No newline at end of file diff --git a/docs/source/_static/test_neoantigen_candidates_annotated.tsv b/docs/source/_static/test_neoantigen_candidates_annotated.tsv index bd846a42..efad173d 100644 --- a/docs/source/_static/test_neoantigen_candidates_annotated.tsv +++ b/docs/source/_static/test_neoantigen_candidates_annotated.tsv @@ -1,3 +1,3 @@ -patientIdentifier gene mutatedXmer wildTypeXmer position dnaVariantAlleleFrequency rnaVariantAlleleFrequency rnaExpression imputedGeneExpression ADN_MHCI ADN_MHCII Amplitude_MHCII_rank Amplitude_MHCI_affinity Amplitude_MHCI_affinity_9mer Best_affinity_MHCII_allele Best_affinity_MHCII_epitope Best_affinity_MHCII_epitope_WT Best_affinity_MHCII_score Best_affinity_MHCII_score_WT Best_affinity_MHCI_9mer_allele Best_affinity_MHCI_9mer_anchor_mutated Best_affinity_MHCI_9mer_epitope Best_affinity_MHCI_9mer_epitope_WT Best_affinity_MHCI_9mer_position_mutation Best_affinity_MHCI_9mer_score Best_affinity_MHCI_9mer_score_WT Best_affinity_MHCI_allele Best_affinity_MHCI_epitope Best_affinity_MHCI_epitope_WT Best_affinity_MHCI_score Best_affinity_MHCI_score_WT Best_rank_MHCII_score Best_rank_MHCII_score_WT Best_rank_MHCII_score_allele Best_rank_MHCII_score_epitope Best_rank_MHCII_score_epitope_WT Best_rank_MHCI_9mer_allele Best_rank_MHCI_9mer_epitope Best_rank_MHCI_9mer_epitope_WT Best_rank_MHCI_9mer_score Best_rank_MHCI_9mer_score_WT Best_rank_MHCI_score Best_rank_MHCI_score_WT Best_rank_MHCI_score_allele Best_rank_MHCI_score_epitope Best_rank_MHCI_score_epitope_WT CDN_MHCI CDN_MHCII DAI_MHCI_affinity Dissimilarity_MHCI Dissimilarity_MHCII Expression_mutated_transcript Generator_rate_ADN_MHCI Generator_rate_ADN_MHCII Generator_rate_CDN_MHCI Generator_rate_CDN_MHCII Generator_rate_MHCI Generator_rate_MHCII Hex_alignment_score_MHCI Hex_alignment_score_MHCII IEDB_Immunogenicity_MHCI IEDB_Immunogenicity_MHCII Improved_Binder_MHCI MixMHC2pred_best_allele MixMHC2pred_best_peptide MixMHC2pred_best_rank MixMHCpred_best_allele MixMHCpred_best_peptide MixMHCpred_best_rank MixMHCpred_best_score Neoag_immunogenicity Number_of_mismatches_MCHI PHBR_I PHBR_II PRIME_best_allele PRIME_best_peptide PRIME_best_rank PRIME_best_score Pathogensimiliarity_MHCII Pathogensimiliarity_MHCI_9mer Priority_score Recognition_Potential_MHCI_9mer Selfsimilarity_MHCI Selfsimilarity_MHCII Selfsimilarity_MHCI_conserved_binder Tcell_predictor_score mutation_not_found_in_proteome vaxrank_binding_score vaxrank_total_score -Ptx BRCA2 AAAAAAAAAAAAALAAAAAAAAAAAAA AAAAAAAAAAAAAFAAAAAAAAAAAAA 14 0.857 NA 0.5195068939999999 0.5365996317 0 1 9 2.3262 0.56811 HLA-DQA1*01:02-DQB1*06:02 AAAAAAAALAAAAAA AAAAAAAAFAAAAAA 15.65 22.47 HLA-C*03:04 0 AAALAAAAA AAAFAAAAA 4 1534.8 1180.8 HLA-A*02:01 AALAAAAAAA AAFAAAAAAA 1018.2 8184.1 0.03 0.27 HLA-DQA1*01:02-DQB1*06:02 AAAAAAAAALAAAAA AAAAAAAAAFAAAAA HLA-B*07:02 AAALAAAAA AAAFAAAAA 4.389 5.068 4.389 5.068 HLA-B*07:02 AAALAAAAA AAAFAAAAA 0 1 7165.9 1 3e-05 0.44522 0 4 0 21 0 25 177 389 0.18182 0.36258 0 HLA-DPA1*01:03-DPB1*06:01 AAAALAAAAAAAAAAA 0.296 HLA-B*07:02 AAALAAAAA 0.3 0.3862 13.16998 1 8.5743 NA HLA-B*07:02 AAALAAAAA 0.04 0.18933 0 0 0 0 0.9763465205057597 0.981263768075705 0.9763465205057597 0.39121828442992074 1 0.01301 0.00579 -Ptx BRCA2 AAAAAAAAAAAAARAAAAAAAAAAAAA AAAAAAAAAAAAAMAAAAAAAAAAAAA 14 0.556 NA 0.715756594 0.5365996317 0 NA NA 5.6409 5.6409 HLA-DQA1*01:02-DQB1*06:02 AAAAAAAAARAAAAA AAAAAAAAAMAAAAA 21.67 14.36 HLA-B*07:02 0 AAAAARAAA AAAAAMAAA 6 199.38 1697.4 HLA-B*07:02 AAAAARAAA AAAAAMAAA 199.38 1697.4 0 1.03 HLA-DPA1*01:03-DPB1*06:01 AAARAAAAAAAAAAA AAAMAAAAAAAAAAA HLA-B*07:02 AAAAARAAA AAAAAMAAA 1.346 4.347 1.346 4.347 HLA-B*07:02 AAAAARAAA AAAAAMAAA 0 1 1498 1 0.00382 0.39796 0 17 0 28 0 45 132 289 0.19477 0.42378 1 HLA-DPA1*01:03-DPB1*06:01 AAAARAAAAAAAAAA 0.0176 HLA-B*07:02 AAAAAARAA 0.08 0.51814 13.16998 1 4.6189 NA HLA-B*07:02 AAAAARAAA 0.06 0.18732 0 0 0.32903 0 0.966307602001344 0.9748046960972773 NA 0.5173265191848659 1 0.80336 0.31971 +patientIdentifier gene mutatedXmer wildTypeXmer position dnaVariantAlleleFrequency rnaVariantAlleleFrequency rnaExpression imputedGeneExpression Alternatively_defined_neopeptide_MHCI Alternatively_defined_neopeptide_MHCII Amplitude_MHCII_bestRank Amplitude_MHCI_bestAffinity Amplitude_MHCI_bestAffinity9mer Classically_defined_neopeptide_MHCI Classically_defined_neopeptide_MHCII DAI_MHCI_bestAffinity Dissimilarity_MHCI Dissimilarity_MHCII GeneratorRate_ADN_MHCI GeneratorRate_ADN_MHCII GeneratorRate_CDN_MHCI GeneratorRate_CDN_MHCII GeneratorRate_MHCI GeneratorRate_MHCII HexAlignmentScore_MHCI HexAlignmentScore_MHCII IEDB_Immunogenicity_MHCI IEDB_Immunogenicity_MHCII Improved_Binder_MHCI MixMHC2pred_bestRank_allele MixMHC2pred_bestRank_peptide MixMHC2pred_bestRank_rank MixMHCpred_bestScore_allele MixMHCpred_bestScore_peptide MixMHCpred_bestScore_rank MixMHCpred_bestScore_score Mutated_imputedGeneExpression_fromDNA Mutated_imputedGeneExpression_fromRNA Mutated_rnaExpression_fromDNA Mutated_rnaExpression_fromRNA NetMHCIIpan_bestAffinity_Of NetMHCIIpan_bestAffinity_affinity NetMHCIIpan_bestAffinity_affinityWT NetMHCIIpan_bestAffinity_allele NetMHCIIpan_bestAffinity_core NetMHCIIpan_bestAffinity_coreRel NetMHCIIpan_bestAffinity_peptide NetMHCIIpan_bestAffinity_peptideWT NetMHCIIpan_bestRank_Of NetMHCIIpan_bestRank_allele NetMHCIIpan_bestRank_core NetMHCIIpan_bestRank_coreRel NetMHCIIpan_bestRank_peptide NetMHCIIpan_bestRank_peptideWT NetMHCIIpan_bestRank_rank NetMHCIIpan_bestRank_rankWT NetMHCpan_bestAffinity9mer_affinity NetMHCpan_bestAffinity9mer_affinityWT NetMHCpan_bestAffinity9mer_allele NetMHCpan_bestAffinity9mer_anchorMutated NetMHCpan_bestAffinity9mer_peptide NetMHCpan_bestAffinity9mer_peptideWT NetMHCpan_bestAffinity9mer_positionMutation NetMHCpan_bestAffinity_Gl NetMHCpan_bestAffinity_Gp NetMHCpan_bestAffinity_Icore NetMHCpan_bestAffinity_Of NetMHCpan_bestAffinity_affinity NetMHCpan_bestAffinity_affinityWT NetMHCpan_bestAffinity_allele NetMHCpan_bestAffinity_core NetMHCpan_bestAffinity_peptide NetMHCpan_bestAffinity_peptideWT NetMHCpan_bestRank9mer_allele NetMHCpan_bestRank9mer_peptide NetMHCpan_bestRank9mer_peptideWT NetMHCpan_bestRank9mer_rank NetMHCpan_bestRank9mer_rankWT NetMHCpan_bestRank_Gl NetMHCpan_bestRank_Gp NetMHCpan_bestRank_Icore NetMHCpan_bestRank_Of NetMHCpan_bestRank_allele NetMHCpan_bestRank_core NetMHCpan_bestRank_peptide NetMHCpan_bestRank_peptideWT NetMHCpan_bestRank_rank NetMHCpan_bestRank_rankWT Number_of_mismatches_MCHI PHBR_I PHBR_II PRIME_best_allele PRIME_best_peptide PRIME_best_rank PRIME_best_score Pathogensimiliarity_MHCII_bestAffinity Pathogensimiliarity_MHCI_bestAffinity Pathogensimiliarity_MHCI_bestAffinity9mer Priority_score_fromDNA Priority_score_fromRNA Priority_score_imputed_fromDNA Priority_score_imputed_fromRNA RecognitionPotential_MHCI_bestAffinity RecognitionPotential_MHCI_bestAffinity9mer Selfsimilarity_MHCI Selfsimilarity_MHCII Selfsimilarity_MHCI_conserved_binder Vaxrank_bindingScore Vaxrank_totalScore Vaxrank_totalScore_imputed mutation_not_found_in_proteome +Ptx BRCA2 AAAAAAAAAAAAALAAAAAAAAAAAAA AAAAAAAAAAAAAFAAAAAAAAAAAAA 14 0.857 0.294 0.519506894 0.5365996317 0 0 0.33333 2.3262 0.56811 0 1 7165.9 1 3e-05 0 1 0 18 0 19 177 389 0.18182 0.36258 0 HLA-DQA1*01:02-DQB1*06:02 AAAAAAALAAAAAAA 0.00438 HLA-B*07:02 AAALAAAAA 0.18911 -0.2706 0.45987 0.15776 0.44522 0.15274 3 14.84 21.8 HLA-DQA1*01:02-DQB1*06:02 AAAAALAAA 0.740 AAAAAAAALAAAAAA AAAAAAAAFAAAAAA 3 HLA-DQA1*03:01-DQB1*06:02 LAAAAAAAA 0.750 AAALAAAAAAAAAAA AAAFAAAAAAAAAAA 0.03 0.01 1534.8 1180.8 HLA-C*03:04 0 AAALAAAAA AAAFAAAAA 4 1 1 AALAAAAAAA 0 1018.2 8184.1 HLA-A*02:01 ALAAAAAAA AALAAAAAAA AAFAAAAAAA HLA-B*07:02 AAALAAAAA AAAFAAAAA 4.389 5.068 0 0 AAALAAAAA 0 HLA-B*07:02 AAALAAAAA AAALAAAAA AAAFAAAAA 4.389 5.068 1 8.5743 NA HLA-B*07:02 AAALAAAAA 0.114 0.10131 0 0 0 0 0 0 0 0 0 0.9763465205057597 0.981263768075705 0.9763465205057597 0.01301 0.00199 0.00205 1 +Ptx BRCA2 AAAAAAAAAAAAARAAAAAAAAAAAAA AAAAAAAAAAAAAMAAAAAAAAAAAAA 14 0.556 0.173 0.715756594 0.5365996317 0 1 390 5.6409 5.6409 0 1 1498 1 0 0 18 0 27 0 45 132 409 0.19477 0.42378 1 HLA-DQA1*01:02-DQB1*06:02 AAAARAAAAAAAAAA 0.00438 HLA-B*07:02 AAAAAARAA 0.08828 0.04913 0.29835 0.09283 0.39796 0.12383 3 13.55 15.81 HLA-DQA1*01:02-DQB1*06:02 AAAARAAAA 0.970 AAAAAAARAAAAAAA AAAAAAAMAAAAAAA 3 HLA-DPA1*01:03-DPB1*06:01 RAAAAAAAA 1.000 AAARAAAAAAAAAAA AAAMAAAAAAAAAAA 0.01 3.9 199.38 1697.4 HLA-B*07:02 0 AAAAARAAA AAAAAMAAA 6 0 0 AAAAARAAA 0 199.38 1697.4 HLA-B*07:02 AAAAARAAA AAAAARAAA AAAAAMAAA HLA-B*07:02 AAAAARAAA AAAAAMAAA 1.346 4.347 0 0 AAAAARAAA 0 HLA-B*07:02 AAAAARAAA AAAAARAAA AAAAAMAAA 1.346 4.347 1 4.6189 NA HLA-B*07:02 AAAAAARAA 0.129 0.09563 0 0 0 0.32903 0.10238 0.26268 0.08173 0 0 0.966307602001344 0.9729918667892427 NA 0.80336 0.09948 0.07458 1 From 3dd6e8f35e6fa326fefff8c23ac17fda72986855 Mon Sep 17 00:00:00 2001 From: Franziska Lang Date: Thu, 11 Jul 2024 09:58:13 +0200 Subject: [PATCH 59/66] increase version --- docs/source/02_installation.md | 6 ++++-- docs/source/conf.py | 2 +- pyproject.toml | 2 +- 3 files changed, 6 insertions(+), 4 deletions(-) diff --git a/docs/source/02_installation.md b/docs/source/02_installation.md index fe53a2ac..79250891 100755 --- a/docs/source/02_installation.md +++ b/docs/source/02_installation.md @@ -8,6 +8,8 @@ This guide contains two alternatives to install NeoFox: The first approach has the lowest entry barrier to use NeoFox as a command line tool. While the second provides access to the command line tool and allows the integration of the NeoFox API. +**NOTE: Please, check the licences of third-party dependencies.** + ## Build and run the docker image Clone the repository: `git clone git@github.com:TRON-Bioinformatics/neofox.git` @@ -170,10 +172,10 @@ export NEOFOX_NETMHC2PAN=`pwd`/netMHCIIpan-4.0/netMHCIIpan ``` Furthermore, a list of available MHC alleles is required. Optionally, you can provide the URL to the IPD-IMGT/HLA database CSV table, see releases here https://www.ebi.ac.uk/ipd/imgt/hla/docs/release.html. -If not provided the default value is the latest version at the time of this writing https://raw.githubusercontent.com/ANHIG/IMGTHLA/Latest/Allelelist.3430.txt +If not provided the default value is the latest version at the time of this writing https://raw.githubusercontent.com/ANHIG/IMGTHLA/Latest/Allelelist.txt ``` -export NEOFOX_HLA_DATABASE=https://raw.githubusercontent.com/ANHIG/IMGTHLA/Latest/Allelelist.3430.txt +export NEOFOX_HLA_DATABASE=https://raw.githubusercontent.com/ANHIG/IMGTHLA/Latest/Allelelist.txt ``` Run the following to configure the NeoFox reference folder: diff --git a/docs/source/conf.py b/docs/source/conf.py index b05a439e..fc530e3a 100644 --- a/docs/source/conf.py +++ b/docs/source/conf.py @@ -24,7 +24,7 @@ # The full version, including alpha/beta/rc tags # TODO: it would be great to have a common versioning, but right now it would require importing neofox here which does # TODO: not work in readthedocs environment -release = "1.1.0" +release = "1.2.0" # -- General configuration --------------------------------------------------- diff --git a/pyproject.toml b/pyproject.toml index d0727915..91d18c0d 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -1,6 +1,6 @@ [tool.poetry] name = "neofox" -version = "1.1.1.dev10" +version = "1.2.0" description = "NEOantigen Feature tOolboX" authors = [ "TRON - Translational Oncology at the University Medical Center", From 910ffcd21dee0d0c713d0fcc9b8e7de5869c9d41 Mon Sep 17 00:00:00 2001 From: Franziska Lang Date: Thu, 11 Jul 2024 10:01:27 +0200 Subject: [PATCH 60/66] remove unnessary file --- neofox/references/install_r_dependencies.R | 3 --- 1 file changed, 3 deletions(-) delete mode 100644 neofox/references/install_r_dependencies.R diff --git a/neofox/references/install_r_dependencies.R b/neofox/references/install_r_dependencies.R deleted file mode 100644 index 2d8f6bc4..00000000 --- a/neofox/references/install_r_dependencies.R +++ /dev/null @@ -1,3 +0,0 @@ -install.packages("caret", repo="http://cran.rstudio.com/") -install.packages("Peptides", repo="http://cran.rstudio.com/") -install.packages("doParallel", repo="http://cran.rstudio.com/") From 62775fa8c22fd2e24aa3ba230c28eb2e2be171de Mon Sep 17 00:00:00 2001 From: Franziska Lang Date: Thu, 11 Jul 2024 10:55:11 +0200 Subject: [PATCH 61/66] update documentation --- docs/source/01_overview.md | 3 +-- docs/source/02_installation.md | 10 +++------- docs/source/03_01_input_data.md | 6 +++--- docs/source/03_02_output_data.md | 5 +++-- docs/source/03_03_usage.md | 2 +- docs/source/04_developer_guide.md | 2 +- 6 files changed, 12 insertions(+), 16 deletions(-) diff --git a/docs/source/01_overview.md b/docs/source/01_overview.md index d7f2c449..17771dab 100644 --- a/docs/source/01_overview.md +++ b/docs/source/01_overview.md @@ -22,7 +22,7 @@ in the last years. NeoFox supports annotation of neoantigen candidates derived from SNVs (single nucleotide variant) and alternative mutation classes such as INDELs or fusion genes. Furthermore, NeoFox supports both human and mouse derived neoantigen candidates. NeoFox covers neoepitope prediction by MHC binding and ligand prediction, similarity/foreignness of a neoepitope candidate sequence, combinatorial features and machine learning approaches. -A list of implemented features and their references are given in Table 1. Please not that some features are currently not available for mouse. +A list of implemented features and their references are given in Table 1. Please note that some features are currently not available for mouse. **Table 1**: Neoantigen features and prioritization algorithms (*§ currently not supported for mouse*) @@ -42,7 +42,6 @@ A list of implemented features and their references are given in Table 1. Please | Recognition potential § | Łuksza et al., 2017, Nature; Balachandran et al, 2017, Nature | https://doi.org/10.1038/nature24473 , https://doi.org/10.1038/nature24462 | | Vaxrank | Rubinsteyn, 2017, Front Immunol | https://doi.org/10.3389/fimmu.2017.01807 | | Priority score | Bjerregaard et al., 2017, Cancer Immunol Immunother. | https://doi.org/10.1007/s00262-017-2001-3 | -| Tcell predictor | Besser et al., 2019, Journal for ImmunoTherapy of Cancer | https://doi.org/10.1186/s40425-019-0595-z | | PRIME v2.0 § | Schmidt et al., 2021, Cell Reports Medicine | https://doi.org/10.1016/j.xcrm.2021.100194 | | HEX § | Chiaro et al., 2021, Cancer Immunology Research | https://doi.org/10.1158/2326-6066.CIR-20-0814 | diff --git a/docs/source/02_installation.md b/docs/source/02_installation.md index 79250891..ca0a0439 100755 --- a/docs/source/02_installation.md +++ b/docs/source/02_installation.md @@ -1,14 +1,10 @@ # Installation This guide contains two alternatives to install NeoFox: -- A set of detailed step by step installation instructions without docker with bioconda or PyPI -- Building a docker image that automates the installation into a container (**NOTE**: the docker recipe is not supported in neofox-v1.1.0. Please use an older version ( For each neoantigen candidate (`mutatedXmer`), the best subsequences (epitopes) based on MHC prediction are annotated. Columns that refer to a predicted epitope consist of 3 components, each separated by "_". The first part contains the tool name, the second part contains the selection method of the best epitope and the third part contains the name of the value. Example: "ToolName_SelectionMethod_ValueName" -> NetMHCpan_bestRank_peptide (literally: the peptide with the best predicted NetMHCpan rank). +> For each neoantigen candidate (`mutatedXmer`), the best predicted epitope based on MHC prediction is annotated. Columns that refer to a predicted epitope consist of 3 components, each separated by "_". The first part contains the tool name, the second part contains the selection method of the best epitope and the third part contains the name of the value. Example: "ToolName_SelectionMethod_ValueName" -> NetMHCpan_bestRank_peptide (literally: the peptide with the best predicted NetMHCpan rank). +If no unique selection of the best predicted epitope can be made due to ties, the lexicographically first epitope is returned. If there are nevertheless two or more alleles that have equally good predicted values for the peptide, the lexicographically first one is reported. These cases can be analysed using the --with-all-epitopes options. The following table describes each of the annotations in the output: @@ -131,7 +132,7 @@ In addition, all logging output is appended to a log file with the suffix "*/.log*", where the folder is set by `--output-folder` and the prefix can be set with `--output-prefix`. -> Note on best predicted epitope selection: If no unique selection of the best predicted epitope can be made due to ties, the lexicographically first epitope is returned. If there are nevertheless two or more alleles that have equally good predicted values for the peptide, the lexicographically first one is reported. These cases can be analysed using the `--with-all-epitopes` options. + ### Tabular format diff --git a/docs/source/03_03_usage.md b/docs/source/03_03_usage.md index cae79a3d..c47c88a7 100755 --- a/docs/source/03_03_usage.md +++ b/docs/source/03_03_usage.md @@ -36,7 +36,7 @@ where: - `--organism`: the organism to which the data corresponds. Possible values: [human, mouse]. Default value: human - `--num-cpus`: number of CPUs to use (*optional*) - `--config`: a config file with the paths to dependencies as shown below (*optional*) -- `--patient-id`: patient identifier (*optional*, this is only relevant if the column `patientIdentifier` is missing in the candidate input file) +- `--patient-id`: patient identifier (*optional*, this is only relevant if the column `patientIdentifier` is missing in the candidate input file and it contains only candidates from one patient) - `--verbose`: get detailed logs **PLEASE NOTE THE FOLLOWING HINTS**: diff --git a/docs/source/04_developer_guide.md b/docs/source/04_developer_guide.md index dd881bcc..12c4df36 100644 --- a/docs/source/04_developer_guide.md +++ b/docs/source/04_developer_guide.md @@ -1,6 +1,6 @@ # Developer guide -> Information: The build system changed in version 1.1.1 to poetry (https://python-poetry.org/). +> Information: The build system changed in NeoFox v1.2.0 to poetry (https://python-poetry.org/). ## Installation of neofox via poetry From 987d8ca0c53a94c4b40db386a92d0b30f242221d Mon Sep 17 00:00:00 2001 From: Franziska Lang Date: Thu, 11 Jul 2024 13:26:30 +0200 Subject: [PATCH 62/66] update doc --- docs/source/02_installation.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/source/02_installation.md b/docs/source/02_installation.md index ca0a0439..e451b971 100755 --- a/docs/source/02_installation.md +++ b/docs/source/02_installation.md @@ -2,7 +2,7 @@ This guide contains two alternatives to install NeoFox: - A set of detailed step by step installation instructions without docker with [bioconda or PyPI](##step-by-step-guide-without-docker) -- Building a [docker image](##build-and-run-the-docker-image) that automates the installation into a container (**NOTE**: the docker recipe is not supported in neofox-v1.1.0. Therefore, we recommend instalation via PyPI or bioconda at the moment. Please use an older version ( Date: Thu, 11 Jul 2024 13:46:03 +0200 Subject: [PATCH 63/66] update docs --- docs/source/02_installation.md | 4 ++-- docs/source/03_03_usage.md | 2 +- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/docs/source/02_installation.md b/docs/source/02_installation.md index e451b971..fefc4620 100755 --- a/docs/source/02_installation.md +++ b/docs/source/02_installation.md @@ -2,9 +2,9 @@ This guide contains two alternatives to install NeoFox: - A set of detailed step by step installation instructions without docker with [bioconda or PyPI](##step-by-step-guide-without-docker) -- Building a [docker image](##build-and-run-the-docker-image) that automates the installation into a container (**NOTE**: the docker recipe is currently not supported.) +- Building a [docker image](##build-and-run-the-docker-image) that automates the installation into a container (**NOTE**: the docker recipe is currently not supported. The docker recipe will be updated soon.) -**NOTE: NeoFox relies on several third-parties dependencies. Please, check the licences of third-party dependencies.** +> NOTE: NeoFox relies on several third-parties dependencies. Please, check the licences of third-party dependencies. ## Build and run the docker image diff --git a/docs/source/03_03_usage.md b/docs/source/03_03_usage.md index c47c88a7..4fc692dc 100755 --- a/docs/source/03_03_usage.md +++ b/docs/source/03_03_usage.md @@ -98,7 +98,7 @@ The neoepitope can be run in two ways: ## Running from docker -**NOTE: The provided docker recipe is not adapted in the current NeoFox version. Please, use a previous version (< Neofox-v1.1.0) at the moment if running from docker is required. The docker recipe will be updated soon.** +**NOTE: The provided docker recipe is not adapted in the current NeoFox version. Please, use NeoFox without docker for now. The docker recipe will be updated soon.** In order to run the command line in a docker image, all of the above applies but some additional steps are required. From 6ae8c847b905e6813e454e5b5104af398ca300e8 Mon Sep 17 00:00:00 2001 From: Franziska Lang Date: Thu, 11 Jul 2024 13:47:05 +0200 Subject: [PATCH 64/66] update docs --- docs/source/04_developer_guide.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/source/04_developer_guide.md b/docs/source/04_developer_guide.md index 12c4df36..61ea508a 100644 --- a/docs/source/04_developer_guide.md +++ b/docs/source/04_developer_guide.md @@ -4,7 +4,7 @@ ## Installation of neofox via poetry -Setup poetry once as described under [Setup poetry](#setup-poetry)) . +Setup poetry once as described under [Setup poetry](#setup-poetry) . Go to your local repository, add poetry to you path and install neofox into a virtual environment managed by poetry. If a `poetry.lock` file is given in the path, herein defined package versions are used. Otherwise the dependencies listed in pyproject.toml are used to generate the environment: From 9ec7c1c2de2581cea06d76fd1c11427b5926381e Mon Sep 17 00:00:00 2001 From: Franziska Lang Date: Thu, 11 Jul 2024 13:52:30 +0200 Subject: [PATCH 65/66] update docs --- docs/source/02_installation.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/source/02_installation.md b/docs/source/02_installation.md index fefc4620..911e5704 100755 --- a/docs/source/02_installation.md +++ b/docs/source/02_installation.md @@ -2,7 +2,7 @@ This guide contains two alternatives to install NeoFox: - A set of detailed step by step installation instructions without docker with [bioconda or PyPI](##step-by-step-guide-without-docker) -- Building a [docker image](##build-and-run-the-docker-image) that automates the installation into a container (**NOTE**: the docker recipe is currently not supported. The docker recipe will be updated soon.) +- Building a [docker image](##build-and-run-the-docker-image) that automates the installation into a container (NOTE: the docker recipe is currently not supported. Therefore, please install NeoFox without docker for now. The docker recipe will be updated soon.) > NOTE: NeoFox relies on several third-parties dependencies. Please, check the licences of third-party dependencies. From 2660a72f4d190474d4c56a58cef793ef7c8f1b71 Mon Sep 17 00:00:00 2001 From: Franziska Lang Date: Thu, 11 Jul 2024 15:04:32 +0200 Subject: [PATCH 66/66] add lxml-html-clean to doc requirements --- docs/requirements.txt | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/docs/requirements.txt b/docs/requirements.txt index 67342ba7..b39fe6f9 100755 --- a/docs/requirements.txt +++ b/docs/requirements.txt @@ -8,4 +8,5 @@ ipykernel==6.4.1 #insipid_sphinx_theme==0.2.1 pydata-sphinx-theme==0.11.0 Jinja2==3.1.2 -markupsafe==2.1.1 \ No newline at end of file +markupsafe==2.1.1 +lxml-html-clean==0.1.1 \ No newline at end of file

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