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Thanks @snyquist2!! So removing the deltas and gammas from the reference fasta got me past that previous error, but now I'm stuck in call-recover_cdr3s:
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18:58:58, 2021-06-21 - TCRGO: Initialized logger, ready for TCRGO.
18:58:58 - INFO: Parsing input data...
18:58:58 - INFO: No index was detected, building index...
18:59:01 - INFO: Reading /cromwell_root/fc-d8b64044-a999-46bf-846b-b52b2a9a1b7c/33d380fe-6f7e-4362-8472-660f91a7f896/TCRGO/ab90f7a0-15d5-4bf3-9329-dce024f1babe/call-alignment/out/pregnancy_test2_repairedsorted.bam...
18:59:01 - INFO: Reading CDR3 positions file and FASTA file...
18:59:01 - ERROR! AE000658|TRAV1-1*01| does not begin with 'TRA' or 'TRB'!
How is the reference fasta you're using syntactically constructed? Or better yet, could you share the human reference fasta you're using?
Thanks @snyquist2!! So removing the deltas and gammas from the reference fasta got me past that previous error, but now I'm stuck in
call-recover_cdr3s
:How is the reference fasta you're using syntactically constructed? Or better yet, could you share the human reference fasta you're using?
Originally posted by @ajwilk in #1 (comment)
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