From 6bb052d9a64b889800b6c1050f2df5e0a4ae319e Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 14 Feb 2024 13:05:25 +1300 Subject: [PATCH 01/65] Added nf-core/fastq_trim_fastp_fastqc subworkflow --- .editorconfig | 6 +- .gitignore | 1 + .pre-commit-config.yaml | 10 + CHANGELOG.md | 4 + CITATION.cff | 2 +- README.md | 2 +- bin/assembly_qc_report_943e0fb.py | 2 +- .../templates/params/params.html | 3 +- conf/modules.config | 26 + conf/test_full.config | 3 + docs/full_run.md | 10 +- modules.json | 19 + modules/nf-core/fastp/environment.yml | 7 + modules/nf-core/fastp/main.nf | 120 +++ modules/nf-core/fastp/meta.yml | 75 ++ modules/nf-core/fastp/tests/main.nf.test | 725 ++++++++++++++++++ modules/nf-core/fastp/tests/main.nf.test.snap | 330 ++++++++ modules/nf-core/fastp/tests/nextflow.config | 6 + modules/nf-core/fastp/tests/tags.yml | 2 + modules/nf-core/fastqc/environment.yml | 7 + modules/nf-core/fastqc/main.nf | 55 ++ modules/nf-core/fastqc/meta.yml | 57 ++ modules/nf-core/fastqc/tests/main.nf.test | 212 +++++ .../nf-core/fastqc/tests/main.nf.test.snap | 88 +++ modules/nf-core/fastqc/tests/tags.yml | 2 + nextflow.config | 5 + subworkflows/local/hic_contact_map.nf | 4 +- .../nf-core/fastq_trim_fastp_fastqc/main.nf | 103 +++ .../nf-core/fastq_trim_fastp_fastqc/meta.yml | 108 +++ version_check.sh | 23 + workflows/assembly_qc.nf | 48 +- 31 files changed, 2035 insertions(+), 30 deletions(-) create mode 100644 modules/nf-core/fastp/environment.yml create mode 100644 modules/nf-core/fastp/main.nf create mode 100644 modules/nf-core/fastp/meta.yml create mode 100644 modules/nf-core/fastp/tests/main.nf.test create mode 100644 modules/nf-core/fastp/tests/main.nf.test.snap create mode 100644 modules/nf-core/fastp/tests/nextflow.config create mode 100644 modules/nf-core/fastp/tests/tags.yml create mode 100644 modules/nf-core/fastqc/environment.yml create mode 100644 modules/nf-core/fastqc/main.nf create mode 100644 modules/nf-core/fastqc/meta.yml create mode 100644 modules/nf-core/fastqc/tests/main.nf.test create mode 100644 modules/nf-core/fastqc/tests/main.nf.test.snap create mode 100644 modules/nf-core/fastqc/tests/tags.yml create mode 100644 subworkflows/nf-core/fastq_trim_fastp_fastqc/main.nf create mode 100644 subworkflows/nf-core/fastq_trim_fastp_fastqc/meta.yml create mode 100755 version_check.sh diff --git a/.editorconfig b/.editorconfig index cf88ed60..e572f526 100644 --- a/.editorconfig +++ b/.editorconfig @@ -11,9 +11,6 @@ indent_style = space [*.{md,yml,yaml,cff}] indent_size = 2 -[*.nf.test] -insert_final_newline = false - [{LICENSE,LEGAL.txt}] charset = unset end_of_line = unset @@ -24,3 +21,6 @@ indent_size = unset [*.{pl,pm}] indent_size = unset + +[*.nf.test] +insert_final_newline = false diff --git a/.gitignore b/.gitignore index 8698da13..fdbb53de 100644 --- a/.gitignore +++ b/.gitignore @@ -6,3 +6,4 @@ work/ results/ *.stdout *.stderr +data/ diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index 82855944..94efb370 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -12,3 +12,13 @@ repos: hooks: - id: editorconfig-checker alias: ec + - repo: local + hooks: + - id: version_checks + name: Version checks + language: system + entry: > + ./version_check.sh + always_run: true + fail_fast: true + pass_filenames: false diff --git a/CHANGELOG.md b/CHANGELOG.md index ded6ca14..2b5675dc 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -1,5 +1,9 @@ # Change Log +## Version 1.4 (14-Feb-2023) + +1. Now it is possible to skip FASTP and FASTQC for the HIC module + ## Version 1.3 (08-Feb-2023) 1. Docker engine is now also supported diff --git a/CITATION.cff b/CITATION.cff index 97253e43..efb1b209 100644 --- a/CITATION.cff +++ b/CITATION.cff @@ -21,7 +21,7 @@ authors: - family-names: "Deng" given-names: "Cecilia" title: "AssemblyQC: A NextFlow pipeline for evaluating assembly quality" -version: 1.3 +version: 1.4 date-released: 2024-02-12 url: "https://github.com/Plant-Food-Research-Open/assembly_qc" doi: 10.5281/zenodo.10647870 diff --git a/README.md b/README.md index 7b126557..11ba59ff 100644 --- a/README.md +++ b/README.md @@ -130,4 +130,4 @@ Cecilia Deng [@CeciliaDeng](https://github.com/CeciliaDeng), Chen Wu [@christina For a comprehensive list of references and versions for the tools, see [CITATIONS.md](./CITATIONS.md). -> Rashid, U., Wu, C., Shiller, J., Smith, K., Crowhurst, R., Davy, M., Chen, T.-H., Thomson, S., & Deng, C. (2024). AssemblyQC: A NextFlow pipeline for evaluating assembly quality (1.3). Zenodo. https://doi.org/10.5281/zenodo.10647870 +> Rashid, U., Wu, C., Shiller, J., Smith, K., Crowhurst, R., Davy, M., Chen, T.-H., Thomson, S., & Deng, C. (2024). AssemblyQC: A NextFlow pipeline for evaluating assembly quality (1.4). Zenodo. https://doi.org/10.5281/zenodo.10647870 diff --git a/bin/assembly_qc_report_943e0fb.py b/bin/assembly_qc_report_943e0fb.py index a862be8d..698026c9 100755 --- a/bin/assembly_qc_report_943e0fb.py +++ b/bin/assembly_qc_report_943e0fb.py @@ -42,7 +42,7 @@ data_from_tools = { **data_from_tools, "VERSIONS": { - "SELF": "v1.3", + "SELF": "v1.4", "NCBI_FCS_ADAPTOR": "0.4", "NCBI_FCS_GX": "0.4", "ASSEMBLATHON_STATS": "github/PlantandFoodResearch/assemblathon2-analysis/a93cba2", diff --git a/bin/report_modules/templates/params/params.html b/bin/report_modules/templates/params/params.html index 6526ab03..20edc093 100644 --- a/bin/report_modules/templates/params/params.html +++ b/bin/report_modules/templates/params/params.html @@ -7,7 +7,8 @@

Reference:

Rashid, U., Wu, C., Shiller, J., Smith, K., Crowhurst, R., Davy, M., Chen, T.-H., Thomson, S., & Deng, C. - (2024). AssemblyQC: A NextFlow pipeline for evaluating assembly quality (1.3). Zenodo. + (2024). AssemblyQC: A NextFlow pipeline for evaluating assembly quality ({{ + all_stats_dicts['VERSIONS']['SELF'] }}). Zenodo. 10.5281/zenodo.10647870. GitHub. https://github.com/Plant-Food-Research-Open/assembly_qc filename.equals("versions.yml") ? null : filename } + ] + } + + withName: FASTQC_TRIM { + publishDir = [ + path: { "${params.outdir}/hic/fastqc_trim" }, + mode: "copy", + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: FASTP { + ext.args = params.hic.fastp_ext_args + publishDir = [ + path: { "${params.outdir}/hic/fastp" }, + mode: "copy", + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + withName: BWA_MEM { ext.prefix = { "${meta.id}.on.${meta.ref_id}.bwa.mem" } ext.args = '-5SP' diff --git a/conf/test_full.config b/conf/test_full.config index 9db94272..d06036b3 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -49,6 +49,9 @@ params { hic { paired_reads = "SRR8238190" + + skip_fastp = 0 + skip_fastqc = 0 } synteny { diff --git a/docs/full_run.md b/docs/full_run.md index b7938a08..c9c46e57 100644 --- a/docs/full_run.md +++ b/docs/full_run.md @@ -154,12 +154,20 @@ kraken2 { Following parameter must be configured: - `paired_reads`: A relative or absolute path to paired reads in fastq.gz format, or a SRA ID. The format for file path is `*R{1,2}*.(fasta|fq).gz`. An example is '/input/genomic/fungal/Neonectria/Genome/20190506_CAGRF19591_CGYCF_HiC/PG_PETUNIA_HiC_CGYCF_CACTCA_L001_R{1,2}.fastq.gz'. +- `skip_fastp`: Skips fastp trimming. +- `skip_fastqc`: Skips QC by fastqc. +- `fastp_ext_args`: Additional arguments for fastp. > ⚙️ From conf/test_full.config ```groovy hic { - paired_reads = "SRR8238190" + paired_reads = "SRR8238190" + + skip_fastp = 0 + skip_fastqc = 0 + + fastp_ext_args = '--qualified_quality_phred 20 --length_required 50' } ``` diff --git a/modules.json b/modules.json index da4cff8c..09dd6642 100644 --- a/modules.json +++ b/modules.json @@ -75,12 +75,31 @@ "https://github.com/nf-core/modules.git": { "modules": { "nf-core": { + "fastp": { + "branch": "master", + "git_sha": "003920c7f9a8ae19b69a97171922880220bedf56", + "installed_by": ["fastq_trim_fastp_fastqc"] + }, + "fastqc": { + "branch": "master", + "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", + "installed_by": ["fastq_trim_fastp_fastqc"] + }, "untar": { "branch": "master", "git_sha": "e719354ba77df0a1bd310836aa2039b45c29d620", "installed_by": ["modules"] } } + }, + "subworkflows": { + "nf-core": { + "fastq_trim_fastp_fastqc": { + "branch": "master", + "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", + "installed_by": ["subworkflows"] + } + } } } } diff --git a/modules/nf-core/fastp/environment.yml b/modules/nf-core/fastp/environment.yml new file mode 100644 index 00000000..70389e66 --- /dev/null +++ b/modules/nf-core/fastp/environment.yml @@ -0,0 +1,7 @@ +name: fastp +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::fastp=0.23.4 diff --git a/modules/nf-core/fastp/main.nf b/modules/nf-core/fastp/main.nf new file mode 100644 index 00000000..96a89f62 --- /dev/null +++ b/modules/nf-core/fastp/main.nf @@ -0,0 +1,120 @@ +process FASTP { + tag "$meta.id" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + 'https://depot.galaxyproject.org/singularity/fastp:0.23.4--h5f740d0_0' : + 'quay.io/biocontainers/fastp:0.23.4--h5f740d0_0' }" + + input: + tuple val(meta), path(reads) + path adapter_fasta + val save_trimmed_fail + val save_merged + + output: + tuple val(meta), path('*.fastp.fastq.gz') , optional:true, emit: reads + tuple val(meta), path('*.json') , emit: json + tuple val(meta), path('*.html') , emit: html + tuple val(meta), path('*.log') , emit: log + path "versions.yml" , emit: versions + tuple val(meta), path('*.fail.fastq.gz') , optional:true, emit: reads_fail + tuple val(meta), path('*.merged.fastq.gz'), optional:true, emit: reads_merged + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def adapter_list = adapter_fasta ? "--adapter_fasta ${adapter_fasta}" : "" + def fail_fastq = save_trimmed_fail && meta.single_end ? "--failed_out ${prefix}.fail.fastq.gz" : save_trimmed_fail && !meta.single_end ? "--unpaired1 ${prefix}_1.fail.fastq.gz --unpaired2 ${prefix}_2.fail.fastq.gz" : '' + // Added soft-links to original fastqs for consistent naming in MultiQC + // Use single ended for interleaved. Add --interleaved_in in config. + if ( task.ext.args?.contains('--interleaved_in') ) { + """ + [ ! -f ${prefix}.fastq.gz ] && ln -sf $reads ${prefix}.fastq.gz + + fastp \\ + --stdout \\ + --in1 ${prefix}.fastq.gz \\ + --thread $task.cpus \\ + --json ${prefix}.fastp.json \\ + --html ${prefix}.fastp.html \\ + $adapter_list \\ + $fail_fastq \\ + $args \\ + 2> >(tee ${prefix}.fastp.log >&2) \\ + | gzip -c > ${prefix}.fastp.fastq.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g") + END_VERSIONS + """ + } else if (meta.single_end) { + """ + [ ! -f ${prefix}.fastq.gz ] && ln -sf $reads ${prefix}.fastq.gz + + fastp \\ + --in1 ${prefix}.fastq.gz \\ + --out1 ${prefix}.fastp.fastq.gz \\ + --thread $task.cpus \\ + --json ${prefix}.fastp.json \\ + --html ${prefix}.fastp.html \\ + $adapter_list \\ + $fail_fastq \\ + $args \\ + 2> >(tee ${prefix}.fastp.log >&2) + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g") + END_VERSIONS + """ + } else { + def merge_fastq = save_merged ? "-m --merged_out ${prefix}.merged.fastq.gz" : '' + """ + [ ! -f ${prefix}_1.fastq.gz ] && ln -sf ${reads[0]} ${prefix}_1.fastq.gz + [ ! -f ${prefix}_2.fastq.gz ] && ln -sf ${reads[1]} ${prefix}_2.fastq.gz + fastp \\ + --in1 ${prefix}_1.fastq.gz \\ + --in2 ${prefix}_2.fastq.gz \\ + --out1 ${prefix}_1.fastp.fastq.gz \\ + --out2 ${prefix}_2.fastp.fastq.gz \\ + --json ${prefix}.fastp.json \\ + --html ${prefix}.fastp.html \\ + $adapter_list \\ + $fail_fastq \\ + $merge_fastq \\ + --thread $task.cpus \\ + --detect_adapter_for_pe \\ + $args \\ + 2> >(tee ${prefix}.fastp.log >&2) + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g") + END_VERSIONS + """ + } + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + def is_single_output = task.ext.args?.contains('--interleaved_in') || meta.single_end + def touch_reads = is_single_output ? "${prefix}.fastp.fastq.gz" : "${prefix}_1.fastp.fastq.gz ${prefix}_2.fastp.fastq.gz" + def touch_merged = (!is_single_output && save_merged) ? "touch ${prefix}.merged.fastq.gz" : "" + """ + touch $touch_reads + touch "${prefix}.fastp.json" + touch "${prefix}.fastp.html" + touch "${prefix}.fastp.log" + $touch_merged + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g") + END_VERSIONS + """ +} diff --git a/modules/nf-core/fastp/meta.yml b/modules/nf-core/fastp/meta.yml new file mode 100644 index 00000000..c22a16ab --- /dev/null +++ b/modules/nf-core/fastp/meta.yml @@ -0,0 +1,75 @@ +name: fastp +description: Perform adapter/quality trimming on sequencing reads +keywords: + - trimming + - quality control + - fastq +tools: + - fastp: + description: | + A tool designed to provide fast all-in-one preprocessing for FastQ files. This tool is developed in C++ with multithreading supported to afford high performance. + documentation: https://github.com/OpenGene/fastp + doi: 10.1093/bioinformatics/bty560 + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information. Use 'single_end: true' to specify single ended or interleaved FASTQs. Use 'single_end: false' for paired-end reads. + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. If you wish to run interleaved paired-end data, supply as single-end data + but with `--interleaved_in` in your `modules.conf`'s `ext.args` for the module. + - adapter_fasta: + type: file + description: File in FASTA format containing possible adapters to remove. + pattern: "*.{fasta,fna,fas,fa}" + - save_trimmed_fail: + type: boolean + description: Specify true to save files that failed to pass trimming thresholds ending in `*.fail.fastq.gz` + - save_merged: + type: boolean + description: Specify true to save all merged reads to the a file ending in `*.merged.fastq.gz` +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: The trimmed/modified/unmerged fastq reads + pattern: "*fastp.fastq.gz" + - json: + type: file + description: Results in JSON format + pattern: "*.json" + - html: + type: file + description: Results in HTML format + pattern: "*.html" + - log: + type: file + description: fastq log file + pattern: "*.log" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - reads_fail: + type: file + description: Reads the failed the preprocessing + pattern: "*fail.fastq.gz" + - reads_merged: + type: file + description: Reads that were successfully merged + pattern: "*.{merged.fastq.gz}" +authors: + - "@drpatelh" + - "@kevinmenden" +maintainers: + - "@drpatelh" + - "@kevinmenden" diff --git a/modules/nf-core/fastp/tests/main.nf.test b/modules/nf-core/fastp/tests/main.nf.test new file mode 100644 index 00000000..7ccd9b59 --- /dev/null +++ b/modules/nf-core/fastp/tests/main.nf.test @@ -0,0 +1,725 @@ +nextflow_process { + + name "Test Process FASTP" + script "../main.nf" + process "FASTP" + tag "modules" + tag "modules_nfcore" + tag "fastp" + + test("test_fastp_single_end") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = false + save_merged = false + + input[0] = Channel.of([ + [ id:'test', single_end:true ], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + def html_text = [ "Q20 bases:12.922000 K (92.984097%)", + "single end (151 cycles)" ] + def log_text = [ "Q20 bases: 12922(92.9841%)", + "reads passed filter: 99" ] + def read_lines = ["@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1", + "TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT", + "AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE + { assert path(process.out.reads.get(0).get(1)).linesGzip.contains(read_line) } + } + }, + { html_text.each { html_part -> + { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } + } + }, + { assert snapshot(process.out.json).match("test_fastp_single_end_json") }, + { log_text.each { log_part -> + { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } + } + }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { file(it[1]).getName() } + + process.out.json.collect { file(it[1]).getName() } + + process.out.html.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.reads_fail.collect { file(it[1]).getName() } + + process.out.reads_merged.collect { file(it[1]).getName() } + ).sort() + ).match("test_fastp_single_end-_match") + }, + { assert snapshot(process.out.versions).match("versions_single_end") } + ) + } + } + + test("test_fastp_single_end-stub") { + + options '-stub' + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = false + save_merged = false + + input[0] = Channel.of([ + [ id:'test', single_end:true ], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + + assertAll( + { assert process.success }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { file(it[1]).getName() } + + process.out.json.collect { file(it[1]).getName() } + + process.out.html.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.reads_fail.collect { file(it[1]).getName() } + + process.out.reads_merged.collect { file(it[1]).getName() } + ).sort() + ).match("test_fastp_single_end-for_stub_match") + }, + { assert snapshot(process.out.versions).match("versions_single_end_stub") } + ) + } + } + + test("test_fastp_paired_end") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = false + save_merged = false + + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + def html_text = [ "Q20 bases:25.719000 K (93.033098%)", + "The input has little adapter percentage (~0.000000%), probably it's trimmed before."] + def log_text = [ "No adapter detected for read1", + "Q30 bases: 12281(88.3716%)"] + def json_text = ['"passed_filter_reads": 198'] + def read1_lines = ["@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1", + "TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT", + "AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE + { assert path(process.out.reads.get(0).get(1).get(0)).linesGzip.contains(read1_line) } + } + }, + { read2_lines.each { read2_line -> + { assert path(process.out.reads.get(0).get(1).get(1)).linesGzip.contains(read2_line) } + } + }, + { html_text.each { html_part -> + { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } + } + }, + { json_text.each { json_part -> + { assert path(process.out.json.get(0).get(1)).getText().contains(json_part) } + } + }, + { log_text.each { log_part -> + { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } + } + }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { it[1].collect { item -> file(item).getName() } } + + process.out.json.collect { file(it[1]).getName() } + + process.out.html.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.reads_fail.collect { file(it[1]).getName() } + + process.out.reads_merged.collect { file(it[1]).getName() } + ).sort() + ).match("test_fastp_paired_end_match") + }, + { assert snapshot(process.out.versions).match("versions_paired_end") } + ) + } + } + + test("test_fastp_paired_end-stub") { + + options '-stub' + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = false + save_merged = false + + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + assertAll( + { assert process.success }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { it[1].collect { item -> file(item).getName() } } + + process.out.json.collect { file(it[1]).getName() } + + process.out.html.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.reads_fail.collect { file(it[1]).getName() } + + process.out.reads_merged.collect { file(it[1]).getName() } + ).sort() + ).match("test_fastp_paired_end-for_stub_match") + }, + { assert snapshot(process.out.versions).match("versions_paired_end-stub") } + ) + } + } + + test("fastp test_fastp_interleaved") { + config './nextflow.config' + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = false + save_merged = false + + input[0] = Channel.of([ + [ id:'test', single_end:true ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) ] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + def html_text = [ "Q20 bases:25.719000 K (93.033098%)", + "paired end (151 cycles + 151 cycles)"] + def log_text = [ "Q20 bases: 12922(92.9841%)", + "reads passed filter: 198"] + def read_lines = [ "@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1", + "TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT", + "AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE + { assert path(process.out.reads.get(0).get(1)).linesGzip.contains(read_line) } + } + }, + { html_text.each { html_part -> + { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } + } + }, + { assert snapshot(process.out.json).match("fastp test_fastp_interleaved_json") }, + { log_text.each { log_part -> + { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } + } + }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { file(it[1]).getName() } + + process.out.json.collect { file(it[1]).getName() } + + process.out.html.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.reads_fail.collect { file(it[1]).getName() } + + process.out.reads_merged.collect { file(it[1]).getName() } + ).sort() + ).match("test_fastp_interleaved-_match") + }, + { assert snapshot(process.out.versions).match("versions_interleaved") } + ) + } + } + + test("fastp test_fastp_interleaved-stub") { + + options '-stub' + + config './nextflow.config' + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = false + save_merged = false + + input[0] = Channel.of([ + [ id:'test', single_end:true ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) ] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + assertAll( + { assert process.success }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { file(it[1]).getName() } + + process.out.json.collect { file(it[1]).getName() } + + process.out.html.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.reads_fail.collect { file(it[1]).getName() } + + process.out.reads_merged.collect { file(it[1]).getName() } + ).sort() + ).match("test_fastp_interleaved-for_stub_match") + }, + { assert snapshot(process.out.versions).match("versions_interleaved-stub") } + ) + } + } + + test("test_fastp_single_end_trim_fail") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = true + save_merged = false + + input[0] = Channel.of([ + [ id:'test', single_end:true ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + def html_text = [ "Q20 bases:12.922000 K (92.984097%)", + "single end (151 cycles)"] + def log_text = [ "Q20 bases: 12922(92.9841%)", + "reads passed filter: 99" ] + def read_lines = [ "@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1", + "TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT", + "AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE + { assert path(process.out.reads.get(0).get(1)).linesGzip.contains(read_line) } + } + }, + { failed_read_lines.each { failed_read_line -> + { assert path(process.out.reads_fail.get(0).get(1)).linesGzip.contains(failed_read_line) } + } + }, + { html_text.each { html_part -> + { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } + } + }, + { assert snapshot(process.out.json).match("test_fastp_single_end_trim_fail_json") }, + { log_text.each { log_part -> + { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } + } + }, + { assert snapshot(process.out.versions).match("versions_single_end_trim_fail") } + ) + } + } + + test("test_fastp_paired_end_trim_fail") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = true + save_merged = false + + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + def html_text = [ "Q20 bases:25.719000 K (93.033098%)", + "The input has little adapter percentage (~0.000000%), probably it's trimmed before."] + def log_text = [ "No adapter detected for read1", + "Q30 bases: 12281(88.3716%)"] + def json_text = ['"passed_filter_reads": 198'] + def read1_lines = ["@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1", + "TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT", + "AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE + { assert path(process.out.reads.get(0).get(1).get(0)).linesGzip.contains(read1_line) } + } + }, + { read2_lines.each { read2_line -> + { assert path(process.out.reads.get(0).get(1).get(1)).linesGzip.contains(read2_line) } + } + }, + { failed_read2_lines.each { failed_read2_line -> + { assert path(process.out.reads_fail.get(0).get(1).get(1)).linesGzip.contains(failed_read2_line) } + } + }, + { html_text.each { html_part -> + { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } + } + }, + { json_text.each { json_part -> + { assert path(process.out.json.get(0).get(1)).getText().contains(json_part) } + } + }, + { log_text.each { log_part -> + { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } + } + }, + { assert snapshot(process.out.versions).match("versions_paired_end_trim_fail") } + ) + } + } + + test("test_fastp_paired_end_merged") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = false + save_merged = true + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + def html_text = [ "

"] + def log_text = [ "Merged and filtered:", + "total reads: 75", + "total bases: 13683"] + def json_text = ['"merged_and_filtered": {', '"total_reads": 75', '"total_bases": 13683'] + def read1_lines = [ "@ERR5069949.1066259 NS500628:121:HK3MMAFX2:1:11312:18369:8333/1", + "CCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTC", + "AAAAAEAEEAEEEEEEEEEEEEEEEEAEEEEAEEEEEEEEAEEEEEEEEEEEEEEEEE/EAEEEEEE/6EEEEEEEEEEAEEAEEE/EE/AEEAEEEEEAEEEA/EEAAEAE + { assert path(process.out.reads.get(0).get(1).get(0)).linesGzip.contains(read1_line) } + } + }, + { read2_lines.each { read2_line -> + { assert path(process.out.reads.get(0).get(1).get(1)).linesGzip.contains(read2_line) } + } + }, + { read_merged_lines.each { read_merged_line -> + { assert path(process.out.reads_merged.get(0).get(1)).linesGzip.contains(read_merged_line) } + } + }, + { html_text.each { html_part -> + { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } + } + }, + { json_text.each { json_part -> + { assert path(process.out.json.get(0).get(1)).getText().contains(json_part) } + } + }, + { log_text.each { log_part -> + { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } + } + }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { it[1].collect { item -> file(item).getName() } } + + process.out.json.collect { file(it[1]).getName() } + + process.out.html.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.reads_fail.collect { file(it[1]).getName() } + + process.out.reads_merged.collect { file(it[1]).getName() } + ).sort() + ).match("test_fastp_paired_end_merged_match") + }, + { assert snapshot(process.out.versions).match("versions_paired_end_merged") } + ) + } + } + + test("test_fastp_paired_end_merged-stub") { + + options '-stub' + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = false + save_merged = true + + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + assertAll( + { assert process.success }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { it[1].collect { item -> file(item).getName() } } + + process.out.json.collect { file(it[1]).getName() } + + process.out.html.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.reads_fail.collect { file(it[1]).getName() } + + process.out.reads_merged.collect { file(it[1]).getName() } + ).sort() + ).match("test_fastp_paired_end_merged-for_stub_match") + }, + { assert snapshot(process.out.versions).match("versions_paired_end_merged_stub") } + ) + } + } + + test("test_fastp_paired_end_merged_adapterlist") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = Channel.of([ file(params.modules_testdata_base_path + 'delete_me/fastp/adapters.fasta', checkIfExists: true) ]) + save_trimmed_fail = false + save_merged = true + + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + def html_text = [ "
"] + def log_text = [ "Merged and filtered:", + "total reads: 75", + "total bases: 13683"] + def json_text = ['"merged_and_filtered": {', '"total_reads": 75', '"total_bases": 13683',"--adapter_fasta"] + def read1_lines = ["@ERR5069949.1066259 NS500628:121:HK3MMAFX2:1:11312:18369:8333/1", + "CCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTC", + "AAAAAEAEEAEEEEEEEEEEEEEEEEAEEEEAEEEEEEEEAEEEEEEEEEEEEEEEEE/EAEEEEEE/6EEEEEEEEEEAEEAEEE/EE/AEEAEEEEEAEEEA/EEAAEAE + { assert path(process.out.reads.get(0).get(1).get(0)).linesGzip.contains(read1_line) } + } + }, + { read2_lines.each { read2_line -> + { assert path(process.out.reads.get(0).get(1).get(1)).linesGzip.contains(read2_line) } + } + }, + { read_merged_lines.each { read_merged_line -> + { assert path(process.out.reads_merged.get(0).get(1)).linesGzip.contains(read_merged_line) } + } + }, + { html_text.each { html_part -> + { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } + } + }, + { json_text.each { json_part -> + { assert path(process.out.json.get(0).get(1)).getText().contains(json_part) } + } + }, + { log_text.each { log_part -> + { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } + } + }, + { assert snapshot(process.out.versions).match("versions_paired_end_merged_adapterlist") } + ) + } + } +} \ No newline at end of file diff --git a/modules/nf-core/fastp/tests/main.nf.test.snap b/modules/nf-core/fastp/tests/main.nf.test.snap new file mode 100644 index 00000000..b4c0e1dd --- /dev/null +++ b/modules/nf-core/fastp/tests/main.nf.test.snap @@ -0,0 +1,330 @@ +{ + "fastp test_fastp_interleaved_json": { + "content": [ + [ + [ + { + "id": "test", + "single_end": true + }, + "test.fastp.json:md5,168f516f7bd4b7b6c32da7cba87299a4" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-17T18:08:06.123035" + }, + "test_fastp_paired_end_merged-for_stub_match": { + "content": [ + [ + [ + "test_1.fastp.fastq.gz", + "test_2.fastp.fastq.gz" + ], + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "test.merged.fastq.gz", + "{id=test, single_end=false}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-17T18:10:13.467574" + }, + "versions_interleaved": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:56:24.615634793" + }, + "test_fastp_single_end_json": { + "content": [ + [ + [ + { + "id": "test", + "single_end": true + }, + "test.fastp.json:md5,c852d7a6dba5819e4ac8d9673bedcacc" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-17T18:06:00.223817" + }, + "versions_paired_end": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:55:42.333545689" + }, + "test_fastp_paired_end_match": { + "content": [ + [ + [ + "test_1.fastp.fastq.gz", + "test_2.fastp.fastq.gz" + ], + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "{id=test, single_end=false}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T12:03:06.431833729" + }, + "test_fastp_interleaved-_match": { + "content": [ + [ + "test.fastp.fastq.gz", + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "{id=test, single_end=true}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T12:03:37.827323085" + }, + "test_fastp_paired_end_merged_match": { + "content": [ + [ + [ + "test_1.fastp.fastq.gz", + "test_2.fastp.fastq.gz" + ], + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "test.merged.fastq.gz", + "{id=test, single_end=false}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T12:08:44.496251446" + }, + "versions_single_end_stub": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:55:27.354051299" + }, + "versions_interleaved-stub": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:56:46.535528418" + }, + "versions_single_end_trim_fail": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:59:03.724591407" + }, + "test_fastp_paired_end-for_stub_match": { + "content": [ + [ + [ + "test_1.fastp.fastq.gz", + "test_2.fastp.fastq.gz" + ], + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "{id=test, single_end=false}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-17T18:07:15.398827" + }, + "versions_paired_end-stub": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:56:06.50017282" + }, + "versions_single_end": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:55:07.67921647" + }, + "versions_paired_end_merged_stub": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:59:47.350653154" + }, + "test_fastp_interleaved-for_stub_match": { + "content": [ + [ + "test.fastp.fastq.gz", + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "{id=test, single_end=true}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-17T18:08:06.127974" + }, + "versions_paired_end_trim_fail": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:59:18.140484878" + }, + "test_fastp_single_end-for_stub_match": { + "content": [ + [ + "test.fastp.fastq.gz", + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "{id=test, single_end=true}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-17T18:06:00.244202" + }, + "test_fastp_single_end-_match": { + "content": [ + [ + "test.fastp.fastq.gz", + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "{id=test, single_end=true}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:57:30.791982648" + }, + "versions_paired_end_merged_adapterlist": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T12:05:37.845370554" + }, + "versions_paired_end_merged": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:59:32.860543858" + }, + "test_fastp_single_end_trim_fail_json": { + "content": [ + [ + [ + { + "id": "test", + "single_end": true + }, + "test.fastp.json:md5,9a7ee180f000e8d00c7fb67f06293eb5" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-17T18:08:41.942317" + } +} \ No newline at end of file diff --git a/modules/nf-core/fastp/tests/nextflow.config b/modules/nf-core/fastp/tests/nextflow.config new file mode 100644 index 00000000..0f7849ad --- /dev/null +++ b/modules/nf-core/fastp/tests/nextflow.config @@ -0,0 +1,6 @@ +process { + + withName: FASTP { + ext.args = "--interleaved_in" + } +} diff --git a/modules/nf-core/fastp/tests/tags.yml b/modules/nf-core/fastp/tests/tags.yml new file mode 100644 index 00000000..c1afcce7 --- /dev/null +++ b/modules/nf-core/fastp/tests/tags.yml @@ -0,0 +1,2 @@ +fastp: + - modules/nf-core/fastp/** diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml new file mode 100644 index 00000000..1787b38a --- /dev/null +++ b/modules/nf-core/fastqc/environment.yml @@ -0,0 +1,7 @@ +name: fastqc +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::fastqc=0.12.1 diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf new file mode 100644 index 00000000..e89d2c47 --- /dev/null +++ b/modules/nf-core/fastqc/main.nf @@ -0,0 +1,55 @@ +process FASTQC { + tag "$meta.id" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + 'https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0' : + 'quay.io/biocontainers/fastqc:0.12.1--hdfd78af_0' }" + + input: + tuple val(meta), path(reads) + + output: + tuple val(meta), path("*.html"), emit: html + tuple val(meta), path("*.zip") , emit: zip + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + // Make list of old name and new name pairs to use for renaming in the bash while loop + def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] } + def rename_to = old_new_pairs*.join(' ').join(' ') + def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ') + """ + printf "%s %s\\n" $rename_to | while read old_name new_name; do + [ -f "\${new_name}" ] || ln -s \$old_name \$new_name + done + + fastqc \\ + $args \\ + --threads $task.cpus \\ + $renamed_files + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.html + touch ${prefix}.zip + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) + END_VERSIONS + """ +} diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml new file mode 100644 index 00000000..ee5507e0 --- /dev/null +++ b/modules/nf-core/fastqc/meta.yml @@ -0,0 +1,57 @@ +name: fastqc +description: Run FastQC on sequenced reads +keywords: + - quality control + - qc + - adapters + - fastq +tools: + - fastqc: + description: | + FastQC gives general quality metrics about your reads. + It provides information about the quality score distribution + across your reads, the per base sequence content (%A/C/G/T). + You get information about adapter contamination and other + overrepresented sequences. + homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ + documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/ + licence: ["GPL-2.0-only"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - html: + type: file + description: FastQC report + pattern: "*_{fastqc.html}" + - zip: + type: file + description: FastQC report archive + pattern: "*_{fastqc.zip}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" + - "@grst" + - "@ewels" + - "@FelixKrueger" +maintainers: + - "@drpatelh" + - "@grst" + - "@ewels" + - "@FelixKrueger" diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test new file mode 100644 index 00000000..31a21314 --- /dev/null +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -0,0 +1,212 @@ +nextflow_process { + + name "Test Process FASTQC" + script "../main.nf" + process "FASTQC" + + tag "modules" + tag "modules_nfcore" + tag "fastqc" + + test("sarscov2 single-end [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [ id: 'test', single_end:true ], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. + // looks like this:
Mon 2 Oct 2023
test.gz
+ // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, + + { assert snapshot(process.out.versions).match("fastqc_versions_single") } + ) + } + } + + test("sarscov2 paired-end [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("File typeConventional base calls") }, + { assert path(process.out.html[0][1][1]).text.contains("File typeConventional base calls") }, + + { assert snapshot(process.out.versions).match("fastqc_versions_paired") } + ) + } + } + + test("sarscov2 interleaved [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, + + { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") } + ) + } + } + + test("sarscov2 paired-end [bam]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, + + { assert snapshot(process.out.versions).match("fastqc_versions_bam") } + ) + } + } + + test("sarscov2 multiple [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, + { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, + { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("File typeConventional base calls") }, + { assert path(process.out.html[0][1][1]).text.contains("File typeConventional base calls") }, + { assert path(process.out.html[0][1][2]).text.contains("File typeConventional base calls") }, + { assert path(process.out.html[0][1][3]).text.contains("File typeConventional base calls") }, + + { assert snapshot(process.out.versions).match("fastqc_versions_multiple") } + ) + } + } + + test("sarscov2 custom_prefix") { + + when { + process { + """ + input[0] = Channel.of([ + [ id:'mysample', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, + + { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") } + ) + } + } + + test("sarscov2 single-end [fastq] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of([ + [ id: 'test', single_end:true ], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.html.collect { file(it[1]).getName() } + + process.out.zip.collect { file(it[1]).getName() } + + process.out.versions ).match("fastqc_stub") } + ) + } + } + +} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap new file mode 100644 index 00000000..86f7c311 --- /dev/null +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -0,0 +1,88 @@ +{ + "fastqc_versions_interleaved": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:07.293713" + }, + "fastqc_stub": { + "content": [ + [ + "test.html", + "test.zip", + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:31:01.425198" + }, + "fastqc_versions_multiple": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:55.797907" + }, + "fastqc_versions_bam": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:26.795862" + }, + "fastqc_versions_single": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:27.043675" + }, + "fastqc_versions_paired": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:47.584191" + }, + "fastqc_versions_custom_prefix": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:41:14.576531" + } +} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/tags.yml b/modules/nf-core/fastqc/tests/tags.yml new file mode 100644 index 00000000..7834294b --- /dev/null +++ b/modules/nf-core/fastqc/tests/tags.yml @@ -0,0 +1,2 @@ +fastqc: + - modules/nf-core/fastqc/** diff --git a/nextflow.config b/nextflow.config index 3450387b..c49c9eb5 100644 --- a/nextflow.config +++ b/nextflow.config @@ -138,6 +138,11 @@ params { // A relative or absolute path to paired reads: *_R{1,2}.(fastq|fq).gz // OR SRA: SRR8238190 paired_reads = "/input/genomic/fungal/Neonectria/Genome/20190506_CAGRF19591_CGYCF_HiC/PG_PETUNIA_HiC_CGYCF_CACTCA_L001_R{1,2}.fastq.gz" + + skip_fastp = 0 + skip_fastqc = 0 + + fastp_ext_args = '--qualified_quality_phred 20 --length_required 50' } synteny { diff --git a/subworkflows/local/hic_contact_map.nf b/subworkflows/local/hic_contact_map.nf index c2629ee5..7c688f12 100644 --- a/subworkflows/local/hic_contact_map.nf +++ b/subworkflows/local/hic_contact_map.nf @@ -7,14 +7,14 @@ include { HIC_QC } from '../../modules/local/hic_qc.nf' workflow HIC_CONTACT_MAP { take: - reads // [ val(id), [ fq ] ] + reads // [ val(meta), [ fq ] ] fasta // [ val(tag), fasta ] main: if (!params.hic.skip) { FASTQ_BWA_MEM_SAMBLASTER( - reads.map { id, fq -> [ [ id: id ], fq ]}, + reads, fasta.map { tag, fasta -> [ [ id: tag ], fasta, [] ] } ) .bam diff --git a/subworkflows/nf-core/fastq_trim_fastp_fastqc/main.nf b/subworkflows/nf-core/fastq_trim_fastp_fastqc/main.nf new file mode 100644 index 00000000..4f1c84fc --- /dev/null +++ b/subworkflows/nf-core/fastq_trim_fastp_fastqc/main.nf @@ -0,0 +1,103 @@ +// +// Read QC and trimming +// + +include { FASTQC as FASTQC_RAW } from '../../../modules/nf-core/fastqc/main' +include { FASTQC as FASTQC_TRIM } from '../../../modules/nf-core/fastqc/main' +include { FASTP } from '../../../modules/nf-core/fastp/main' + +// +// Function that parses fastp json output file to get total number of reads after trimming +// +import groovy.json.JsonSlurper + +def getFastpReadsAfterFiltering(json_file) { + def Map json = (Map) new JsonSlurper().parseText(json_file.text).get('summary') + return json['after_filtering']['total_reads'].toLong() +} + +workflow FASTQ_TRIM_FASTP_FASTQC { + take: + ch_reads // channel: [ val(meta), path(reads) ] + ch_adapter_fasta // channel: [ path(fasta) ] + val_save_trimmed_fail // value: boolean + val_save_merged // value: boolean + val_skip_fastp // value: boolean + val_skip_fastqc // value: boolean + + main: + + ch_versions = Channel.empty() + + ch_fastqc_raw_html = Channel.empty() + ch_fastqc_raw_zip = Channel.empty() + if (!val_skip_fastqc) { + FASTQC_RAW ( + ch_reads + ) + ch_fastqc_raw_html = FASTQC_RAW.out.html + ch_fastqc_raw_zip = FASTQC_RAW.out.zip + ch_versions = ch_versions.mix(FASTQC_RAW.out.versions.first()) + } + + ch_trim_reads = ch_reads + ch_trim_json = Channel.empty() + ch_trim_html = Channel.empty() + ch_trim_log = Channel.empty() + ch_trim_reads_fail = Channel.empty() + ch_trim_reads_merged = Channel.empty() + ch_fastqc_trim_html = Channel.empty() + ch_fastqc_trim_zip = Channel.empty() + if (!val_skip_fastp) { + FASTP ( + ch_reads, + ch_adapter_fasta, + val_save_trimmed_fail, + val_save_merged + ) + ch_trim_reads = FASTP.out.reads + ch_trim_json = FASTP.out.json + ch_trim_html = FASTP.out.html + ch_trim_log = FASTP.out.log + ch_trim_reads_fail = FASTP.out.reads_fail + ch_trim_reads_merged = FASTP.out.reads_merged + ch_versions = ch_versions.mix(FASTP.out.versions.first()) + + // + // Filter empty FastQ files after adapter trimming so FastQC doesn't fail + // + ch_trim_reads + .join(ch_trim_json) + .map { + meta, reads, json -> + if (getFastpReadsAfterFiltering(json) > 0) { + [ meta, reads ] + } + } + .set { ch_trim_reads } + + if (!val_skip_fastqc) { + FASTQC_TRIM ( + ch_trim_reads + ) + ch_fastqc_trim_html = FASTQC_TRIM.out.html + ch_fastqc_trim_zip = FASTQC_TRIM.out.zip + ch_versions = ch_versions.mix(FASTQC_TRIM.out.versions.first()) + } + } + + emit: + reads = ch_trim_reads // channel: [ val(meta), path(reads) ] + trim_json = ch_trim_json // channel: [ val(meta), path(json) ] + trim_html = ch_trim_html // channel: [ val(meta), path(html) ] + trim_log = ch_trim_log // channel: [ val(meta), path(log) ] + trim_reads_fail = ch_trim_reads_fail // channel: [ val(meta), path(fastq.gz) ] + trim_reads_merged = ch_trim_reads_merged // channel: [ val(meta), path(fastq.gz) ] + + fastqc_raw_html = ch_fastqc_raw_html // channel: [ val(meta), path(html) ] + fastqc_raw_zip = ch_fastqc_raw_zip // channel: [ val(meta), path(zip) ] + fastqc_trim_html = ch_fastqc_trim_html // channel: [ val(meta), path(html) ] + fastqc_trim_zip = ch_fastqc_trim_zip // channel: [ val(meta), path(zip) ] + + versions = ch_versions.ifEmpty(null) // channel: [ path(versions.yml) ] +} diff --git a/subworkflows/nf-core/fastq_trim_fastp_fastqc/meta.yml b/subworkflows/nf-core/fastq_trim_fastp_fastqc/meta.yml new file mode 100644 index 00000000..9f4e12e0 --- /dev/null +++ b/subworkflows/nf-core/fastq_trim_fastp_fastqc/meta.yml @@ -0,0 +1,108 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "fastq_trim_fastp_fastqc" +description: Read QC, fastp trimming and read qc +keywords: + - qc + - quality_control + - adapters + - trimming + - fastq +components: + - fastqc + - fastp +input: + - ch_reads: + type: file + description: | + Structure: [ val(meta), path (reads) ] + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ], List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. If you wish to run interleaved paired-end data, supply as single-end data + but with `--interleaved_in` in your `modules.conf`'s `ext.args` for the module. + - ch_adapter_fasta: + type: file + description: | + Structure: path(adapter_fasta) + File in FASTA format containing possible adapters to remove. + - val_save_trimmed_fail: + type: boolean + description: | + Structure: val(save_trimmed_fail) + Specify true to save files that failed to pass trimming thresholds ending in `*.fail.fastq.gz` + - val_save_merged: + type: boolean + description: | + Structure: val(save_merged) + Specify true to save all merged reads to the a file ending in `*.merged.fastq.gz` + - val_skip_fastqc: + type: boolean + description: | + Structure: val(skip_fastqc) + skip the fastqc process if true + - val_skip_fastp: + type: boolean + description: | + Structure: val(skip_fastp) + skip the fastp process if true +output: + - meta: + type: value + description: Groovy Map containing sample information e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + Structure: [ val(meta), path(reads) ] + The trimmed/modified/unmerged fastq reads + - trim_json: + type: file + description: | + Structure: [ val(meta), path(trim_json) ] + Results in JSON format + - trim_html: + type: file + description: | + Structure: [ val(meta), path(trim_html) ] + Results in HTML format + - trim_log: + type: file + description: | + Structure: [ val(meta), path(trim_log) ] + fastq log file + - trim_reads_fail: + type: file + description: | + Structure: [ val(meta), path(trim_reads_fail) ] + Reads the failed the preprocessing + - trim_reads_merged: + type: file + description: | + Structure: [ val(meta), path(trim_reads_merged) ] + Reads that were successfully merged + - fastqc_raw_html: + type: file + description: | + Structure: [ val(meta), path(fastqc_raw_html) ] + Raw fastQC report + - fastqc_raw_zip: + type: file + description: | + Structure: [ val(meta), path(fastqc_raw_zip) ] + Raw fastQC report archive + - fastqc_trim_html: + type: file + description: | + Structure: [ val(meta), path(fastqc_trim_html) ] + Trimmed fastQC report + - fastqc_trim_zip: + type: file + description: | + Structure: [ val(meta), path(fastqc_trim_zip) ] + Trimmed fastQC report archive + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@Joon-Klaps" +maintainers: + - "@Joon-Klaps" diff --git a/version_check.sh b/version_check.sh new file mode 100755 index 00000000..1af5c650 --- /dev/null +++ b/version_check.sh @@ -0,0 +1,23 @@ +#!/usr/bin/env bash + +report_version=$(sed -n '/"SELF": /s/"SELF": "v//p' ./bin/assembly_qc_report_943e0fb.py | sed 's/",//' | tr -d '[:space:]') +cff_version=$(sed -n '/^version: /s/version: //p' CITATION.cff | tr -d '[:space:]') + +if [[ $report_version != $cff_version ]]; then + echo 'report_version != cff_version' + exit 1 +fi + +# Check README version + +readme_citation_text=$(grep -A10 '## Citations' README.md) + +if [[ ! $readme_citation_text =~ "($report_version)" ]]; then + echo 'Failed to match README version' + exit 1 +fi + +# Check CHANGELOG version + +grep "Version $report_version" CHANGELOG.md >/dev/null \ + || (echo 'Failed to match CHANGELOG version'; exit 1) diff --git a/workflows/assembly_qc.nf b/workflows/assembly_qc.nf index fb4ea013..8ef70d26 100644 --- a/workflows/assembly_qc.nf +++ b/workflows/assembly_qc.nf @@ -1,24 +1,24 @@ nextflow.enable.dsl=2 -include {validateParams } from '../modules/local/utils.nf' -include {jsonifyParams } from '../modules/local/utils.nf' - -include { VALIDATE_FASTA } from '../subworkflows/local/validate_fasta.nf' -include { VALIDATE_GFF3 } from '../subworkflows/local/validate_gff3.nf' -include { BUSCO } from '../subworkflows/local/busco.nf' -include { TIDK } from '../subworkflows/local/tidk.nf' -include { FASTA_LTRRETRIEVER_LAI} from '../subworkflows/pfr/fasta_ltrretriever_lai/main.nf' -include { KRAKEN2 } from '../subworkflows/local/kraken2.nf' -include { NCBI_FCS_ADAPTOR } from '../subworkflows/local/ncbi_fcs_adaptor.nf' -include { NCBI_FCS_GX } from '../subworkflows/local/ncbi_fcs_gx.nf' -include { HIC_PREPROCESS } from '../subworkflows/local/hic_preprocess.nf' -include { HIC_CONTACT_MAP } from '../subworkflows/local/hic_contact_map.nf' -include { SYNTENY } from '../subworkflows/local/synteny.nf' - -include { CREATE_REPORT } from '../modules/local/create_report.nf' -include { ASSEMBLATHON_STATS } from '../modules/local/assemblathon_stats.nf' -include { GENOMETOOLS_GT_STAT } from '../modules/local/genometools_gt_stat.nf' -include { BIOCODE_GFF3_STATS } from '../modules/local/biocode_gff3_stats.nf' +include { validateParams } from '../modules/local/utils.nf' +include { jsonifyParams } from '../modules/local/utils.nf' + +include { VALIDATE_FASTA } from '../subworkflows/local/validate_fasta.nf' +include { VALIDATE_GFF3 } from '../subworkflows/local/validate_gff3.nf' +include { BUSCO } from '../subworkflows/local/busco.nf' +include { TIDK } from '../subworkflows/local/tidk.nf' +include { FASTA_LTRRETRIEVER_LAI } from '../subworkflows/pfr/fasta_ltrretriever_lai/main.nf' +include { KRAKEN2 } from '../subworkflows/local/kraken2.nf' +include { NCBI_FCS_ADAPTOR } from '../subworkflows/local/ncbi_fcs_adaptor.nf' +include { NCBI_FCS_GX } from '../subworkflows/local/ncbi_fcs_gx.nf' +include { FASTQ_TRIM_FASTP_FASTQC } from '../subworkflows/nf-core/fastq_trim_fastp_fastqc/main' +include { HIC_CONTACT_MAP } from '../subworkflows/local/hic_contact_map.nf' +include { SYNTENY } from '../subworkflows/local/synteny.nf' + +include { CREATE_REPORT } from '../modules/local/create_report.nf' +include { ASSEMBLATHON_STATS } from '../modules/local/assemblathon_stats.nf' +include { GENOMETOOLS_GT_STAT } from '../modules/local/genometools_gt_stat.nf' +include { BIOCODE_GFF3_STATS } from '../modules/local/biocode_gff3_stats.nf' validateParams(params) def paramsAsJSON = jsonifyParams(params) @@ -144,7 +144,15 @@ workflow ASSEMBLY_QC { ch_paired_reads = Channel.empty() } - HIC_PREPROCESS(ch_paired_reads) + FASTQ_TRIM_FASTP_FASTQC( + ch_paired_reads.map { sample, fq -> [ [ id: sample, single_end: false], fq ] }, + [], + true, // val_save_trimmed_fail + false, // val_save_merged + params.hic.skip_fastp, + params.hic.skip_fastqc + ) + .reads | set { ch_cleaned_paired_reads } HIC_CONTACT_MAP( From a88808ac4e3f3976ae594a0c6c0ff2d7df3f821f Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 14 Feb 2024 13:10:14 +1300 Subject: [PATCH 02/65] Removed old HIC_PREPROCESS subworkflow --- subworkflows/local/hic_preprocess.nf | 73 ---------------------------- 1 file changed, 73 deletions(-) delete mode 100644 subworkflows/local/hic_preprocess.nf diff --git a/subworkflows/local/hic_preprocess.nf b/subworkflows/local/hic_preprocess.nf deleted file mode 100644 index 59607e8f..00000000 --- a/subworkflows/local/hic_preprocess.nf +++ /dev/null @@ -1,73 +0,0 @@ -nextflow.enable.dsl=2 - -workflow HIC_PREPROCESS { - take: - paired_reads - - main: - if (!params.hic.skip) { - FASTP(paired_reads) - | set { ch_cleaned_paired_reads } - - paired_reads - .join(ch_cleaned_paired_reads, remainder: true) - | FAST_QC - } else { - ch_cleaned_paired_reads = Channel.of([]) - } - - emit: - cleaned_paired_reads = ch_cleaned_paired_reads -} - -process FASTP { - tag "$sample_id" - label "process_medium" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/fastp:0.23.2--h5f740d0_3': - 'quay.io/biocontainers/fastp:0.23.2--h5f740d0_3' }" - - input: - tuple val(sample_id), path(reads) - - output: - tuple val(sample_id), path('*.fastp.fastq.gz') - - script: - """ - fastp \ - -i ${reads[0]} \ - -o "\$(basename ${reads[0]} .fastq.gz).fastp.fastq.gz" \ - -I ${reads[1]} \ - -O "\$(basename ${reads[1]} .fastq.gz).fastp.fastq.gz" \ - --qualified_quality_phred 20 \ - --length_required 50 \ - --thread ${task.cpus} - """ -} - - -process FAST_QC { - tag "$sample_id" - label "process_medium" - - publishDir "${params.outdir}/hic/fastqc", mode:'copy' - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/fastqc:0.11.9--hdfd78af_1': - 'quay.io/biocontainers/fastqc:0.11.9--hdfd78af_1' }" - - input: - tuple val(sample_id), path(raw_reads), path(clean_reads) - - output: - path '*.html' - path '*.zip' - - script: - """ - fastqc ${raw_reads} ${clean_reads} \ - -t ${task.cpus} \ - --nogroup - """ -} From 391011cffe98c9a0ed3635bc81a14816449f8d97 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 14 Feb 2024 13:15:34 +1300 Subject: [PATCH 03/65] Updated fastqc and fastp versions --- CITATIONS.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/CITATIONS.md b/CITATIONS.md index 00c1e7ad..3a80b8e8 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -80,9 +80,9 @@ - HIC CONTACT MAP - JUICEBOX.JS (2.4.3, [MIT](https://github.com/igvteam/juicebox.js/blob/master/LICENSE)) > Robinson JT, Turner D, Durand NC, Thorvaldsdóttir H, Mesirov JP, Aiden EL. 2018. Juicebox.js Provides a Cloud-Based Visualization System for Hi-C Data. Cell Syst. 2018 Feb 28;6(2):256-258.e1. doi: . Epub 2018 Feb 7. PMID: 29428417; PMCID: PMC6047755. - - FASTP (0.23.2, [MIT](https://github.com/OpenGene/fastp/blob/master/LICENSE)) + - FASTP (0.23.4, [MIT](https://github.com/OpenGene/fastp/blob/master/LICENSE)) > Chen S, Zhou Y, Chen Y, Gu J. 2018. fastp: an ultra-fast all-in-one FASTQ preprocessor, Bioinformatics, Volume 34, Issue 17, 01 September 2018, Pages i884–i890, - - FASTQC (0.11.9, [GPL v3](https://github.com/s-andrews/FastQC/blob/master/LICENSE.txt)) + - FASTQC (0.12.9, [GPL v3](https://github.com/s-andrews/FastQC/blob/master/LICENSE.txt)) > - RUN_ASSEMBLY_VISUALIZER (commit: 63029aa, [MIT](https://github.com/aidenlab/3d-dna/blob/master/LICENSE)) > Dudchenko O, Batra SS, Omer AD, Nyquist SK, Hoeger M, Durand NC, Shamim MS, Machol I, Lander, Aiden AP, Aiden EL 2017. De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds.Science356, 92-95(2017). doi: . Available at: From 7fe4d3da8b487483eaac3af24eec08e102cf85ee Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 14 Feb 2024 14:52:11 +1300 Subject: [PATCH 04/65] Now using nf-core/FASTA_EXPLORE_SEARCH_PLOT_TIDK --- CHANGELOG.md | 2 + bin/report_modules/parsers/tidk_parser.py | 18 +- conf/modules.config | 63 +++ main.nf | 8 +- modules.json | 30 ++ modules/nf-core/seqkit/seq/environment.yml | 9 + modules/nf-core/seqkit/seq/main.nf | 63 +++ modules/nf-core/seqkit/seq/meta.yml | 48 +++ modules/nf-core/seqkit/seq/tests/main.nf.test | 149 +++++++ .../seqkit/seq/tests/main.nf.test.snap | 126 ++++++ .../nf-core/seqkit/seq/tests/nextflow.config | 3 + modules/nf-core/seqkit/seq/tests/tags.yml | 2 + modules/nf-core/seqkit/sort/environment.yml | 9 + modules/nf-core/seqkit/sort/main.nf | 63 +++ modules/nf-core/seqkit/sort/meta.yml | 45 ++ .../nf-core/seqkit/sort/tests/main.nf.test | 149 +++++++ .../seqkit/sort/tests/main.nf.test.snap | 126 ++++++ .../nf-core/seqkit/sort/tests/nextflow.config | 3 + modules/nf-core/seqkit/sort/tests/tags.yml | 2 + modules/nf-core/tidk/explore/environment.yml | 9 + modules/nf-core/tidk/explore/main.nf | 57 +++ modules/nf-core/tidk/explore/meta.yml | 52 +++ .../nf-core/tidk/explore/tests/main.nf.test | 86 ++++ .../tidk/explore/tests/main.nf.test.snap | 68 +++ .../tidk/explore/tests/nextflow.config | 3 + modules/nf-core/tidk/explore/tests/tags.yml | 2 + modules/nf-core/tidk/plot/environment.yml | 9 + modules/nf-core/tidk/plot/main.nf | 47 +++ modules/nf-core/tidk/plot/meta.yml | 47 +++ modules/nf-core/tidk/plot/tests/main.nf.test | 74 ++++ .../nf-core/tidk/plot/tests/main.nf.test.snap | 68 +++ modules/nf-core/tidk/plot/tests/tags.yml | 2 + modules/nf-core/tidk/search/environment.yml | 9 + modules/nf-core/tidk/search/main.nf | 62 +++ modules/nf-core/tidk/search/meta.yml | 51 +++ .../nf-core/tidk/search/tests/main.nf.test | 119 ++++++ .../tidk/search/tests/main.nf.test.snap | 36 ++ .../nf-core/tidk/search/tests/nextflow.config | 3 + modules/nf-core/tidk/search/tests/tags.yml | 2 + .../fasta_explore_search_plot_tidk/main.nf | 90 ++++ .../fasta_explore_search_plot_tidk/meta.yml | 74 ++++ .../tests/main.nf.test | 119 ++++++ .../tests/main.nf.test.snap | 389 ++++++++++++++++++ .../tests/nextflow.config | 19 + .../tests/tags.yml | 2 + workflows/{assembly_qc.nf => assemblyqc.nf} | 66 ++- 46 files changed, 2450 insertions(+), 33 deletions(-) create mode 100644 modules/nf-core/seqkit/seq/environment.yml create mode 100644 modules/nf-core/seqkit/seq/main.nf create mode 100644 modules/nf-core/seqkit/seq/meta.yml create mode 100644 modules/nf-core/seqkit/seq/tests/main.nf.test create mode 100644 modules/nf-core/seqkit/seq/tests/main.nf.test.snap create mode 100644 modules/nf-core/seqkit/seq/tests/nextflow.config create mode 100644 modules/nf-core/seqkit/seq/tests/tags.yml create mode 100644 modules/nf-core/seqkit/sort/environment.yml create mode 100644 modules/nf-core/seqkit/sort/main.nf create mode 100644 modules/nf-core/seqkit/sort/meta.yml create mode 100644 modules/nf-core/seqkit/sort/tests/main.nf.test create mode 100644 modules/nf-core/seqkit/sort/tests/main.nf.test.snap create mode 100644 modules/nf-core/seqkit/sort/tests/nextflow.config create mode 100644 modules/nf-core/seqkit/sort/tests/tags.yml create mode 100644 modules/nf-core/tidk/explore/environment.yml create mode 100644 modules/nf-core/tidk/explore/main.nf create mode 100644 modules/nf-core/tidk/explore/meta.yml create mode 100644 modules/nf-core/tidk/explore/tests/main.nf.test create mode 100644 modules/nf-core/tidk/explore/tests/main.nf.test.snap create mode 100644 modules/nf-core/tidk/explore/tests/nextflow.config create mode 100644 modules/nf-core/tidk/explore/tests/tags.yml create mode 100644 modules/nf-core/tidk/plot/environment.yml create mode 100644 modules/nf-core/tidk/plot/main.nf create mode 100644 modules/nf-core/tidk/plot/meta.yml create mode 100644 modules/nf-core/tidk/plot/tests/main.nf.test create mode 100644 modules/nf-core/tidk/plot/tests/main.nf.test.snap create mode 100644 modules/nf-core/tidk/plot/tests/tags.yml create mode 100644 modules/nf-core/tidk/search/environment.yml create mode 100644 modules/nf-core/tidk/search/main.nf create mode 100644 modules/nf-core/tidk/search/meta.yml create mode 100644 modules/nf-core/tidk/search/tests/main.nf.test create mode 100644 modules/nf-core/tidk/search/tests/main.nf.test.snap create mode 100644 modules/nf-core/tidk/search/tests/nextflow.config create mode 100644 modules/nf-core/tidk/search/tests/tags.yml create mode 100644 subworkflows/nf-core/fasta_explore_search_plot_tidk/main.nf create mode 100644 subworkflows/nf-core/fasta_explore_search_plot_tidk/meta.yml create mode 100644 subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test create mode 100644 subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test.snap create mode 100644 subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/nextflow.config create mode 100644 subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/tags.yml rename workflows/{assembly_qc.nf => assemblyqc.nf} (66%) diff --git a/CHANGELOG.md b/CHANGELOG.md index 2b5675dc..36170a52 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,6 +3,8 @@ ## Version 1.4 (14-Feb-2023) 1. Now it is possible to skip FASTP and FASTQC for the HIC module +2. Renamed ASSEMBLY_QC workflow to ASSEMBLYQC +3. Now using nf-core/FASTA_EXPLORE_SEARCH_PLOT_TIDK ## Version 1.3 (08-Feb-2023) diff --git a/bin/report_modules/parsers/tidk_parser.py b/bin/report_modules/parsers/tidk_parser.py index c12c5adf..556d93a4 100644 --- a/bin/report_modules/parsers/tidk_parser.py +++ b/bin/report_modules/parsers/tidk_parser.py @@ -30,35 +30,35 @@ def parse_tidk_folder(folder_name="tidk_outputs"): plot_url = f"data:image/{ext}+xml;base64,{base64_utf8_str}" file_tokens = re.findall( - r"([\w]+).tidk.plot(.empty)?.svg", + r"([\w]+).([\w]+).svg", os.path.basename(str(plot_path)), )[0] - if "_a_posteriori" in file_tokens[0]: - hap_str_literal = file_tokens[0].replace("_a_posteriori", "") - sequence_file_name = f"{hap_str_literal}.a_posteriori.sequence" + sample_tag = file_tokens[0].strip() + plot_type = file_tokens[1].strip() + if "aposteriori" in plot_type: + sequence_file_name = f"{sample_tag}.top.sequence.txt" with open(f"{dir}/{folder_name}/{sequence_file_name}", "r") as file: lines = file.readlines() sequence = "" if len(lines) < 1 else lines[0].strip() - display_name = f"{hap_str_literal}: a posteriori sequence" + display_name = f"{sample_tag}: a posteriori sequence" else: - hap_str_literal = file_tokens[0].replace("_a_priori", "") - display_name = f"{hap_str_literal}: a priori sequence" + display_name = f"{sample_tag}: a priori sequence" sequence = "" data["TIDK"].append( { - "hap": file_tokens[0], + "hap": f"{sample_tag}_{plot_type}", "hap_display": display_name, "sequence": sequence, "is_a_priori": "a priori" in display_name, "a_priori_sequence": a_priori_sequence, "has_sequence": sequence != "", "tidk_plot": plot_url, - "tidk_plot_empty": file_tokens[1] != "", + "tidk_plot_empty": False, } ) diff --git a/conf/modules.config b/conf/modules.config index ff8c7f24..71a2e696 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -1,3 +1,66 @@ +if(!params.tidk.skip) { + process { + + withName: FILTER_BY_LENGTH { + ext.args = params.tidk.filter_by_size ? "-m ${params.tidk.filter_size_bp}" : '' + ext.prefix = { "${meta.id}.filtered" } + } + + withName: SORT_BY_LENGTH { + ext.args = '--quiet --reverse --by-length' + ext.prefix = { "${meta.id}.sorted" } + } + + withName: TIDK_EXPLORE { + ext.args = '--minimum 5 --maximum 30' + publishDir = [ + path: { "${params.outdir}/tidk" }, + mode: "copy", + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: TIDK_SEARCH_APRIORI { + ext.prefix = { "${meta.id}.apriori" } + ext.args = '--extension tsv' + publishDir = [ + path: { "${params.outdir}/tidk" }, + mode: "copy", + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: TIDK_SEARCH_APOSTERIORI { + ext.prefix = { "${meta.id}.aposteriori" } + ext.args = '--extension tsv' + publishDir = [ + path: { "${params.outdir}/tidk" }, + mode: "copy", + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: TIDK_PLOT_APRIORI { + ext.prefix = { "${meta.id}.apriori" } + publishDir = [ + path: { "${params.outdir}/tidk" }, + mode: "copy", + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: TIDK_PLOT_APOSTERIORI { + ext.prefix = { "${meta.id}.aposteriori" } + publishDir = [ + path: { "${params.outdir}/tidk" }, + mode: "copy", + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + } +} + + if(!params.lai.skip) { process { diff --git a/main.nf b/main.nf index 6d7dc34c..af0fa69e 100755 --- a/main.nf +++ b/main.nf @@ -2,8 +2,12 @@ nextflow.enable.dsl=2 -include { ASSEMBLY_QC } from './workflows/assembly_qc.nf' +include { ASSEMBLYQC } from './workflows/assemblyqc.nf' workflow { - ASSEMBLY_QC() + PFR_ASSEMBLYQC() +} + +workflow PFR_ASSEMBLYQC { + ASSEMBLYQC() } diff --git a/modules.json b/modules.json index 09dd6642..be08b97d 100644 --- a/modules.json +++ b/modules.json @@ -85,6 +85,31 @@ "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", "installed_by": ["fastq_trim_fastp_fastqc"] }, + "seqkit/seq": { + "branch": "master", + "git_sha": "687ad41c14008d3d55cf7c2ffacebe6a057211a4", + "installed_by": ["fasta_explore_search_plot_tidk"] + }, + "seqkit/sort": { + "branch": "master", + "git_sha": "ffcdfb354f8c8e59bdccc1a4249f0f3b232c2a3d", + "installed_by": ["fasta_explore_search_plot_tidk"] + }, + "tidk/explore": { + "branch": "master", + "git_sha": "fad335b31b32d78f89a8340373d3ccfc0f2b18b5", + "installed_by": ["fasta_explore_search_plot_tidk"] + }, + "tidk/plot": { + "branch": "master", + "git_sha": "d54cd1d823f151854825da8ffe7289bcbf77206b", + "installed_by": ["fasta_explore_search_plot_tidk"] + }, + "tidk/search": { + "branch": "master", + "git_sha": "669a329f4aa37f5b7f03776c2ed1cd0ef122c626", + "installed_by": ["fasta_explore_search_plot_tidk"] + }, "untar": { "branch": "master", "git_sha": "e719354ba77df0a1bd310836aa2039b45c29d620", @@ -94,6 +119,11 @@ }, "subworkflows": { "nf-core": { + "fasta_explore_search_plot_tidk": { + "branch": "master", + "git_sha": "2b21fbeb20ad9f17612f4a3dd7b12971513f08d5", + "installed_by": ["subworkflows"] + }, "fastq_trim_fastp_fastqc": { "branch": "master", "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", diff --git a/modules/nf-core/seqkit/seq/environment.yml b/modules/nf-core/seqkit/seq/environment.yml new file mode 100644 index 00000000..9019d269 --- /dev/null +++ b/modules/nf-core/seqkit/seq/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "seqkit_seq" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::seqkit=2.6.1" diff --git a/modules/nf-core/seqkit/seq/main.nf b/modules/nf-core/seqkit/seq/main.nf new file mode 100644 index 00000000..d05da23d --- /dev/null +++ b/modules/nf-core/seqkit/seq/main.nf @@ -0,0 +1,63 @@ +process SEQKIT_SEQ { + tag "$meta.id" + label 'process_low' + // File IO can be a bottleneck. See: https://bioinf.shenwei.me/seqkit/usage/#parallelization-of-cpu-intensive-jobs + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + 'https://depot.galaxyproject.org/singularity/seqkit:2.6.1--h9ee0642_0': + 'quay.io/biocontainers/seqkit:2.6.1--h9ee0642_0' }" + + input: + tuple val(meta), path(fastx) + + output: + tuple val(meta), path("${prefix}.*") , emit: fastx + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + prefix = task.ext.prefix ?: "${meta.id}" + def extension = "fastq" + if ("$fastx" ==~ /.+\.fasta|.+\.fasta.gz|.+\.fa|.+\.fa.gz|.+\.fas|.+\.fas.gz|.+\.fna|.+\.fna.gz|.+\.fsa|.+\.fsa.gz/ ) { + extension = "fasta" + } + extension = fastx.toString().endsWith('.gz') ? "${extension}.gz" : extension + def call_gzip = extension.endsWith('.gz') ? "| gzip -c $args2" : '' + if("${prefix}.${extension}" == "$fastx") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ + seqkit \\ + seq \\ + --threads $task.cpus \\ + $args \\ + $fastx \\ + $call_gzip \\ + > ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + seqkit: \$(seqkit version | cut -d' ' -f2) + END_VERSIONS + """ + + stub: + prefix = task.ext.prefix ?: "${meta.id}" + def extension = "fastq" + if ("$fastx" ==~ /.+\.fasta|.+\.fasta.gz|.+\.fa|.+\.fa.gz|.+\.fas|.+\.fas.gz|.+\.fna|.+\.fna.gz|.+\.fsa|.+\.fsa.gz/ ) { + extension = "fasta" + } + extension = fastx.toString().endsWith('.gz') ? "${extension}.gz" : extension + if("${prefix}.${extension}" == "$fastx") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ + touch ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + seqkit: \$(seqkit version | cut -d' ' -f2) + END_VERSIONS + """ +} diff --git a/modules/nf-core/seqkit/seq/meta.yml b/modules/nf-core/seqkit/seq/meta.yml new file mode 100644 index 00000000..8d4e2b16 --- /dev/null +++ b/modules/nf-core/seqkit/seq/meta.yml @@ -0,0 +1,48 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "seqkit_seq" +description: Transforms sequences (extract ID, filter by length, remove gaps, reverse complement...) +keywords: + - genomics + - fasta + - fastq + - transform + - filter + - gaps + - complement +tools: + - "seqkit": + description: "A cross-platform and ultrafast toolkit for FASTA/Q file manipulation" + homepage: "https://bioinf.shenwei.me/seqkit/" + documentation: "https://bioinf.shenwei.me/seqkit/usage/" + tool_dev_url: "https://github.com/shenwei356/seqkit" + doi: "10.1371/journal.pone.0163962" + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fastx: + type: file + description: Input fasta/fastq file + pattern: "*.{fsa,fas,fa,fasta,fastq,fq,fsa.gz,fas.gz,fa.gz,fasta.gz,fastq.gz,fq.gz}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fastx: + type: file + description: Output fasta/fastq file + pattern: "*.{fasta,fasta.gz,fastq,fastq.gz}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/nf-core/seqkit/seq/tests/main.nf.test b/modules/nf-core/seqkit/seq/tests/main.nf.test new file mode 100644 index 00000000..304d1a67 --- /dev/null +++ b/modules/nf-core/seqkit/seq/tests/main.nf.test @@ -0,0 +1,149 @@ +nextflow_process { + + name "Test Process SEQKIT_SEQ" + script "../main.nf" + process "SEQKIT_SEQ" + config './nextflow.config' + + tag "modules" + tag "modules_nfcore" + tag "seqkit" + tag "seqkit/seq" + + test("sarscov2-genome_fasta") { + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("sarscov2-genome_fasta_gz") { + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta_gz'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("sarscov2-test_1_fastq_gz") { + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("file_name_conflict-fail_with_error") { + when { + process { + """ + input[0] = [ + [ id:'test_1' ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert !process.success }, + { assert process.stdout.toString().contains("Input and output names are the same") } + ) + } + + } + + test("sarscov2-genome_fasta-stub") { + + options '-stub' + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("file_name_conflict-fail_with_error-stub") { + + options '-stub' + + when { + process { + """ + input[0] = [ + [ id:'genome' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert !process.success }, + { assert process.stdout.toString().contains("Input and output names are the same") } + ) + } + + } + +} \ No newline at end of file diff --git a/modules/nf-core/seqkit/seq/tests/main.nf.test.snap b/modules/nf-core/seqkit/seq/tests/main.nf.test.snap new file mode 100644 index 00000000..91b4d17a --- /dev/null +++ b/modules/nf-core/seqkit/seq/tests/main.nf.test.snap @@ -0,0 +1,126 @@ +{ + "sarscov2-genome_fasta-stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.fasta:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" + ], + "fastx": [ + [ + { + "id": "test" + }, + "test.fasta:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" + ] + } + ], + "timestamp": "2023-12-18T10:34:00.37449" + }, + "versions": { + "content": [ + [ + "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" + ] + ], + "timestamp": "2023-12-17T13:56:53.318962" + }, + "sarscov2-test_1_fastq_gz": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.fastq.gz:md5,4161df271f9bfcd25d5845a1e220dbec" + ] + ], + "1": [ + "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" + ], + "fastx": [ + [ + { + "id": "test" + }, + "test.fastq.gz:md5,4161df271f9bfcd25d5845a1e220dbec" + ] + ], + "versions": [ + "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" + ] + } + ], + "timestamp": "2023-12-18T10:33:53.528342" + }, + "sarscov2-genome_fasta": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.fasta:md5,483f4a5dfe60171c86ee9b7e6dff908b" + ] + ], + "1": [ + "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" + ], + "fastx": [ + [ + { + "id": "test" + }, + "test.fasta:md5,483f4a5dfe60171c86ee9b7e6dff908b" + ] + ], + "versions": [ + "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" + ] + } + ], + "timestamp": "2023-12-18T10:33:44.757686" + }, + "sarscov2-genome_fasta_gz": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.fasta.gz:md5,483f4a5dfe60171c86ee9b7e6dff908b" + ] + ], + "1": [ + "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" + ], + "fastx": [ + [ + { + "id": "test" + }, + "test.fasta.gz:md5,483f4a5dfe60171c86ee9b7e6dff908b" + ] + ], + "versions": [ + "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" + ] + } + ], + "timestamp": "2023-12-18T10:33:49.115171" + } +} \ No newline at end of file diff --git a/modules/nf-core/seqkit/seq/tests/nextflow.config b/modules/nf-core/seqkit/seq/tests/nextflow.config new file mode 100644 index 00000000..d8e3c66a --- /dev/null +++ b/modules/nf-core/seqkit/seq/tests/nextflow.config @@ -0,0 +1,3 @@ +process { + ext.args2 = '-n' +} diff --git a/modules/nf-core/seqkit/seq/tests/tags.yml b/modules/nf-core/seqkit/seq/tests/tags.yml new file mode 100644 index 00000000..5eeca7e3 --- /dev/null +++ b/modules/nf-core/seqkit/seq/tests/tags.yml @@ -0,0 +1,2 @@ +seqkit/seq: + - "modules/nf-core/seqkit/seq/**" diff --git a/modules/nf-core/seqkit/sort/environment.yml b/modules/nf-core/seqkit/sort/environment.yml new file mode 100644 index 00000000..820c5707 --- /dev/null +++ b/modules/nf-core/seqkit/sort/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "seqkit_sort" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::seqkit=2.6.1" diff --git a/modules/nf-core/seqkit/sort/main.nf b/modules/nf-core/seqkit/sort/main.nf new file mode 100644 index 00000000..ae2485e1 --- /dev/null +++ b/modules/nf-core/seqkit/sort/main.nf @@ -0,0 +1,63 @@ +process SEQKIT_SORT { + tag "$meta.id" + label 'process_low' + // File IO can be a bottleneck. See: https://bioinf.shenwei.me/seqkit/usage/#parallelization-of-cpu-intensive-jobs + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + 'https://depot.galaxyproject.org/singularity/seqkit:2.6.1--h9ee0642_0': + 'quay.io/biocontainers/seqkit:2.6.1--h9ee0642_0' }" + + input: + tuple val(meta), path(fastx) + + output: + tuple val(meta), path("${prefix}.*") , emit: fastx + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + prefix = task.ext.prefix ?: "${meta.id}" + def extension = "fastq" + if ("$fastx" ==~ /.+\.fasta|.+\.fasta.gz|.+\.fa|.+\.fa.gz|.+\.fas|.+\.fas.gz|.+\.fna|.+\.fna.gz|.+\.fsa|.+\.fsa.gz/ ) { + extension = "fasta" + } + extension = fastx.toString().endsWith('.gz') ? "${extension}.gz" : extension + def call_gzip = extension.endsWith('.gz') ? "| gzip -c $args2 " : '' + if("${prefix}.${extension}" == "$fastx") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ + seqkit \\ + sort \\ + --threads $task.cpus \\ + $args \\ + $fastx \\ + $call_gzip \\ + > ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + seqkit: \$(seqkit version | cut -d' ' -f2) + END_VERSIONS + """ + + stub: + prefix = task.ext.prefix ?: "${meta.id}" + def extension = "fastq" + if ("$fastx" ==~ /.+\.fasta|.+\.fasta.gz|.+\.fa|.+\.fa.gz|.+\.fas|.+\.fas.gz|.+\.fna|.+\.fna.gz|.+\.fsa|.+\.fsa.gz/ ) { + extension = "fasta" + } + extension = fastx.toString().endsWith('.gz') ? "${extension}.gz" : extension + if("${prefix}.${extension}" == "$fastx") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ + touch ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + seqkit: \$(seqkit version | cut -d' ' -f2) + END_VERSIONS + """ +} diff --git a/modules/nf-core/seqkit/sort/meta.yml b/modules/nf-core/seqkit/sort/meta.yml new file mode 100644 index 00000000..2e61ce15 --- /dev/null +++ b/modules/nf-core/seqkit/sort/meta.yml @@ -0,0 +1,45 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "seqkit_sort" +description: Sorts sequences by id/name/sequence/length +keywords: + - genomics + - fasta + - fastq + - sort +tools: + - "seqkit": + description: "A cross-platform and ultrafast toolkit for FASTA/Q file manipulation" + homepage: "https://bioinf.shenwei.me/seqkit/" + documentation: "https://bioinf.shenwei.me/seqkit/usage/" + tool_dev_url: "https://github.com/shenwei356/seqkit" + doi: "10.1371/journal.pone.0163962" + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fastx: + type: file + description: Input fasta/fastq file + pattern: "*.{fsa,fas,fa,fasta,fastq,fq,fsa.gz,fas.gz,fa.gz,fasta.gz,fastq.gz,fq.gz}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fastx: + type: file + description: Output fasta/fastq file + pattern: "*.{fasta.gz,fastq.gz}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/nf-core/seqkit/sort/tests/main.nf.test b/modules/nf-core/seqkit/sort/tests/main.nf.test new file mode 100644 index 00000000..19857b32 --- /dev/null +++ b/modules/nf-core/seqkit/sort/tests/main.nf.test @@ -0,0 +1,149 @@ +nextflow_process { + + name "Test Process SEQKIT_SORT" + script "../main.nf" + process "SEQKIT_SORT" + config './nextflow.config' + + tag "modules" + tag "modules_nfcore" + tag "seqkit" + tag "seqkit/sort" + + test("sarscov2-genome_fasta") { + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("sarscov2-genome_fasta_gz") { + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta_gz'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("sarscov2-test_1_fastq_gz") { + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("file_name_conflict-fail_with_error") { + when { + process { + """ + input[0] = [ + [ id:'test_1' ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert !process.success }, + { assert process.stdout.toString().contains("Input and output names are the same,") } + ) + } + + } + + test("sarscov2-genome_fasta-stub") { + + options '-stub' + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("file_name_conflict-fail_with_error-stub") { + + options '-stub' + + when { + process { + """ + input[0] = [ + [ id:'genome' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert !process.success }, + { assert process.stdout.toString().contains("Input and output names are the same") } + ) + } + + } + +} \ No newline at end of file diff --git a/modules/nf-core/seqkit/sort/tests/main.nf.test.snap b/modules/nf-core/seqkit/sort/tests/main.nf.test.snap new file mode 100644 index 00000000..94e2cb35 --- /dev/null +++ b/modules/nf-core/seqkit/sort/tests/main.nf.test.snap @@ -0,0 +1,126 @@ +{ + "sarscov2-genome_fasta-stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.fasta:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" + ], + "fastx": [ + [ + { + "id": "test" + }, + "test.fasta:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" + ] + } + ], + "timestamp": "2023-12-18T10:07:19.28815" + }, + "versions": { + "content": [ + [ + "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" + ] + ], + "timestamp": "2023-12-18T10:07:15.341516" + }, + "sarscov2-test_1_fastq_gz": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.fastq.gz:md5,80d9fd1976648214f44e42bc3d705972" + ] + ], + "1": [ + "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" + ], + "fastx": [ + [ + { + "id": "test" + }, + "test.fastq.gz:md5,80d9fd1976648214f44e42bc3d705972" + ] + ], + "versions": [ + "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" + ] + } + ], + "timestamp": "2023-12-18T10:12:25.704893" + }, + "sarscov2-genome_fasta": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.fasta:md5,483f4a5dfe60171c86ee9b7e6dff908b" + ] + ], + "1": [ + "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" + ], + "fastx": [ + [ + { + "id": "test" + }, + "test.fasta:md5,483f4a5dfe60171c86ee9b7e6dff908b" + ] + ], + "versions": [ + "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" + ] + } + ], + "timestamp": "2023-12-18T10:07:15.293713" + }, + "sarscov2-genome_fasta_gz": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.fasta.gz:md5,483f4a5dfe60171c86ee9b7e6dff908b" + ] + ], + "1": [ + "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" + ], + "fastx": [ + [ + { + "id": "test" + }, + "test.fasta.gz:md5,483f4a5dfe60171c86ee9b7e6dff908b" + ] + ], + "versions": [ + "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" + ] + } + ], + "timestamp": "2023-12-18T10:09:20.306713" + } +} \ No newline at end of file diff --git a/modules/nf-core/seqkit/sort/tests/nextflow.config b/modules/nf-core/seqkit/sort/tests/nextflow.config new file mode 100644 index 00000000..d8e3c66a --- /dev/null +++ b/modules/nf-core/seqkit/sort/tests/nextflow.config @@ -0,0 +1,3 @@ +process { + ext.args2 = '-n' +} diff --git a/modules/nf-core/seqkit/sort/tests/tags.yml b/modules/nf-core/seqkit/sort/tests/tags.yml new file mode 100644 index 00000000..c839b58c --- /dev/null +++ b/modules/nf-core/seqkit/sort/tests/tags.yml @@ -0,0 +1,2 @@ +seqkit/sort: + - "modules/nf-core/seqkit/sort/**" diff --git a/modules/nf-core/tidk/explore/environment.yml b/modules/nf-core/tidk/explore/environment.yml new file mode 100644 index 00000000..4fe55407 --- /dev/null +++ b/modules/nf-core/tidk/explore/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "tidk_explore" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::tidk=0.2.41" diff --git a/modules/nf-core/tidk/explore/main.nf b/modules/nf-core/tidk/explore/main.nf new file mode 100644 index 00000000..c8baeca8 --- /dev/null +++ b/modules/nf-core/tidk/explore/main.nf @@ -0,0 +1,57 @@ +process TIDK_EXPLORE { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + 'https://depot.galaxyproject.org/singularity/tidk:0.2.41--hdbdd923_0': + 'quay.io/biocontainers/tidk:0.2.41--hdbdd923_0' }" + + input: + tuple val(meta), path(fasta) + + output: + tuple val(meta), path("*.tidk.explore.tsv") , emit: explore_tsv + tuple val(meta), path("*.top.sequence.txt") , emit: top_sequence, optional: true + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + tidk \\ + explore \\ + $args \\ + $fasta \\ + > ${prefix}.tidk.explore.tsv + + [[ \$(cat ${prefix}.tidk.explore.tsv | wc -l) -gt 1 ]] \\ + && cat \\ + ${prefix}.tidk.explore.tsv \\ + | sed -n 2p \\ + | awk '{print \$1;}' \\ + > ${prefix}.top.sequence.txt \\ + || echo "No sequence identified" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tidk: \$(tidk --version | sed 's/tidk //') + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.tidk.explore.tsv + touch ${prefix}.top.sequence.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tidk: \$(tidk --version | sed 's/tidk //') + END_VERSIONS + """ +} diff --git a/modules/nf-core/tidk/explore/meta.yml b/modules/nf-core/tidk/explore/meta.yml new file mode 100644 index 00000000..582aaf56 --- /dev/null +++ b/modules/nf-core/tidk/explore/meta.yml @@ -0,0 +1,52 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "tidk_explore" +description: | + `tidk explore` attempts to find the simple telomeric repeat unit in the genome provided. + It will report this repeat in its canonical form (e.g. TTAGG -> AACCT). +keywords: + - genomics + - telomere + - search +tools: + - "tidk": + description: tidk is a toolkit to identify and visualise telomeric repeats in genomes + homepage: "https://github.com/tolkit/telomeric-identifier" + documentation: "https://github.com/tolkit/telomeric-identifier" + tool_dev_url: "https://github.com/tolkit/telomeric-identifier" + doi: "10.5281/zenodo.10091385" + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fasta: + type: file + description: The input fasta file + pattern: "*.{fsa,fa,fasta}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - explore_tsv: + type: file + description: Telomeres and their frequencies in TSV format + pattern: "*.tidk.explore.tsv" + - top_sequence: + type: file + description: | + The most frequent telomere sequence if one or more + sequences are identified by the toolkit + pattern: "*.top.sequence.txt" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/nf-core/tidk/explore/tests/main.nf.test b/modules/nf-core/tidk/explore/tests/main.nf.test new file mode 100644 index 00000000..9f00f522 --- /dev/null +++ b/modules/nf-core/tidk/explore/tests/main.nf.test @@ -0,0 +1,86 @@ +nextflow_process { + + name "Test Process TIDK_EXPLORE" + script "../main.nf" + process "TIDK_EXPLORE" + config "./nextflow.config" + + tag "modules" + tag "modules_nfcore" + tag "tidk" + tag "tidk/explore" + + test("homo_sapiens-genome_21_fasta-success") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("homo_sapiens-genome_fasta-no_top_sequence") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.explore_tsv).match("no_top_sequence_explore_tsv") }, + { assert process.out.top_sequence == [] }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("stub") { + + options '-stub' + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert process.out.explore_tsv != null }, + { assert process.out.top_sequence != null }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + +} \ No newline at end of file diff --git a/modules/nf-core/tidk/explore/tests/main.nf.test.snap b/modules/nf-core/tidk/explore/tests/main.nf.test.snap new file mode 100644 index 00000000..c46d31c1 --- /dev/null +++ b/modules/nf-core/tidk/explore/tests/main.nf.test.snap @@ -0,0 +1,68 @@ +{ + "homo_sapiens-genome_21_fasta-success": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.tidk.explore.tsv:md5,89de91ef36eb0925aefca61757f5275f" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.top.sequence.txt:md5,bd63900958df06516e45b887072d788f" + ] + ], + "2": [ + "versions.yml:md5,8de27958aee1d1fc9075e8046114bdb5" + ], + "explore_tsv": [ + [ + { + "id": "test" + }, + "test.tidk.explore.tsv:md5,89de91ef36eb0925aefca61757f5275f" + ] + ], + "top_sequence": [ + [ + { + "id": "test" + }, + "test.top.sequence.txt:md5,bd63900958df06516e45b887072d788f" + ] + ], + "versions": [ + "versions.yml:md5,8de27958aee1d1fc9075e8046114bdb5" + ] + } + ], + "timestamp": "2023-12-05T14:13:24.14906" + }, + "no_top_sequence_explore_tsv": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.tidk.explore.tsv:md5,27b8513be5478ea2b846a407b3639c01" + ] + ] + ], + "timestamp": "2023-12-05T14:16:18.982423" + }, + "versions": { + "content": [ + [ + "versions.yml:md5,8de27958aee1d1fc9075e8046114bdb5" + ] + ], + "timestamp": "2023-12-05T14:13:24.198311" + } +} \ No newline at end of file diff --git a/modules/nf-core/tidk/explore/tests/nextflow.config b/modules/nf-core/tidk/explore/tests/nextflow.config new file mode 100644 index 00000000..d8f33fe0 --- /dev/null +++ b/modules/nf-core/tidk/explore/tests/nextflow.config @@ -0,0 +1,3 @@ +process { + ext.args = '--minimum 5 --maximum 30' +} diff --git a/modules/nf-core/tidk/explore/tests/tags.yml b/modules/nf-core/tidk/explore/tests/tags.yml new file mode 100644 index 00000000..af666655 --- /dev/null +++ b/modules/nf-core/tidk/explore/tests/tags.yml @@ -0,0 +1,2 @@ +tidk/explore: + - "modules/nf-core/tidk/explore/**" diff --git a/modules/nf-core/tidk/plot/environment.yml b/modules/nf-core/tidk/plot/environment.yml new file mode 100644 index 00000000..ed1706e3 --- /dev/null +++ b/modules/nf-core/tidk/plot/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "tidk_plot" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::tidk=0.2.41" diff --git a/modules/nf-core/tidk/plot/main.nf b/modules/nf-core/tidk/plot/main.nf new file mode 100644 index 00000000..0282f1c9 --- /dev/null +++ b/modules/nf-core/tidk/plot/main.nf @@ -0,0 +1,47 @@ +process TIDK_PLOT { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + 'https://depot.galaxyproject.org/singularity/tidk:0.2.41--hdbdd923_0': + 'quay.io/biocontainers/tidk:0.2.41--hdbdd923_0' }" + + input: + tuple val(meta), path(tsv) + + output: + tuple val(meta), path("*.svg"), emit: svg + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + tidk \\ + plot \\ + --output $prefix \\ + $args \\ + --tsv "$tsv" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tidk: \$(tidk --version | sed 's/tidk //') + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.svg + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tidk: \$(tidk --version | sed 's/tidk //') + END_VERSIONS + """ +} diff --git a/modules/nf-core/tidk/plot/meta.yml b/modules/nf-core/tidk/plot/meta.yml new file mode 100644 index 00000000..451195c8 --- /dev/null +++ b/modules/nf-core/tidk/plot/meta.yml @@ -0,0 +1,47 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "tidk_plot" +description: | + Plots telomeric repeat frequency against sliding window location + using data produced by `tidk/search` +keywords: + - genomics + - telomere + - search + - plot +tools: + - "tidk": + description: tidk is a toolkit to identify and visualise telomeric repeats in genomes + homepage: "https://github.com/tolkit/telomeric-identifier" + documentation: "https://github.com/tolkit/telomeric-identifier" + tool_dev_url: "https://github.com/tolkit/telomeric-identifier" + doi: "10.5281/zenodo.10091385" + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - tsv: + type: file + description: Search results in TSV format from `tidk search` + pattern: "*.tsv" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - svg: + type: file + description: Telomere search plot + pattern: "*.svg" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/nf-core/tidk/plot/tests/main.nf.test b/modules/nf-core/tidk/plot/tests/main.nf.test new file mode 100644 index 00000000..179c537e --- /dev/null +++ b/modules/nf-core/tidk/plot/tests/main.nf.test @@ -0,0 +1,74 @@ +nextflow_process { + + name "Test Process TIDK_PLOT" + script "../main.nf" + process "TIDK_PLOT" + + tag "modules" + tag "modules_nfcore" + tag "tidk" + tag "tidk/search" + tag "tidk/plot" + + test("homo_sapiens-genome_21_fasta-success") { + + setup { + run("TIDK_SEARCH") { + script "../../../../nf-core/tidk/search" + + process { + """ + input[0] = [ + [ id:'test' ], + file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) + ] + input[1] = 'TTAGGG' + """ + } + } + } + + when { + process { + """ + input[0] = TIDK_SEARCH.out.tsv + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("stub") { + + options '-stub' + + when { + process { + """ + input[0] = [ + [ id:'test' ], + [] + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + +} \ No newline at end of file diff --git a/modules/nf-core/tidk/plot/tests/main.nf.test.snap b/modules/nf-core/tidk/plot/tests/main.nf.test.snap new file mode 100644 index 00000000..5de9533e --- /dev/null +++ b/modules/nf-core/tidk/plot/tests/main.nf.test.snap @@ -0,0 +1,68 @@ +{ + "homo_sapiens-genome_21_fasta-success": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.svg:md5,e76985fdc220867a05967984c2c4675d" + ] + ], + "1": [ + "versions.yml:md5,b850b0d8b9add80d7863cc62e3f32c99" + ], + "svg": [ + [ + { + "id": "test" + }, + "test.svg:md5,e76985fdc220867a05967984c2c4675d" + ] + ], + "versions": [ + "versions.yml:md5,b850b0d8b9add80d7863cc62e3f32c99" + ] + } + ], + "timestamp": "2023-12-05T14:29:24.721706" + }, + "versions": { + "content": [ + [ + "versions.yml:md5,b850b0d8b9add80d7863cc62e3f32c99" + ] + ], + "timestamp": "2023-12-05T14:29:55.412674" + }, + "stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.svg:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + "versions.yml:md5,b850b0d8b9add80d7863cc62e3f32c99" + ], + "svg": [ + [ + { + "id": "test" + }, + "test.svg:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,b850b0d8b9add80d7863cc62e3f32c99" + ] + } + ], + "timestamp": "2023-12-16T18:50:29.344319" + } +} \ No newline at end of file diff --git a/modules/nf-core/tidk/plot/tests/tags.yml b/modules/nf-core/tidk/plot/tests/tags.yml new file mode 100644 index 00000000..91228695 --- /dev/null +++ b/modules/nf-core/tidk/plot/tests/tags.yml @@ -0,0 +1,2 @@ +tidk/plot: + - "modules/nf-core/tidk/plot/**" diff --git a/modules/nf-core/tidk/search/environment.yml b/modules/nf-core/tidk/search/environment.yml new file mode 100644 index 00000000..eaf92acc --- /dev/null +++ b/modules/nf-core/tidk/search/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "tidk_search" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::tidk=0.2.41" diff --git a/modules/nf-core/tidk/search/main.nf b/modules/nf-core/tidk/search/main.nf new file mode 100644 index 00000000..20f402e2 --- /dev/null +++ b/modules/nf-core/tidk/search/main.nf @@ -0,0 +1,62 @@ +process TIDK_SEARCH { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + 'https://depot.galaxyproject.org/singularity/tidk:0.2.41--hdbdd923_0': + 'quay.io/biocontainers/tidk:0.2.41--hdbdd923_0' }" + + input: + tuple val(meta), path(fasta) + val string + + output: + tuple val(meta), path("*.tsv") , emit: tsv , optional: true + tuple val(meta), path("*.bedgraph") , emit: bedgraph , optional: true + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + tidk \\ + search \\ + --string $string \\ + --output $prefix \\ + --dir tidk \\ + $args \\ + $fasta + + mv \\ + tidk/${prefix}_telomeric_repeat_windows.tsv \\ + ${prefix}.tsv \\ + || echo "TSV file was not produced" + + mv \\ + tidk/${prefix}_telomeric_repeat_windows.bedgraph \\ + ${prefix}.bedgraph \\ + || echo "BEDGRAPH file was not produced" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tidk: \$(tidk --version | sed 's/tidk //') + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def extension = args.contains("--extension bedgraph") ? 'bedgraph' : 'tsv' + """ + touch ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tidk: \$(tidk --version | sed 's/tidk //') + END_VERSIONS + """ +} diff --git a/modules/nf-core/tidk/search/meta.yml b/modules/nf-core/tidk/search/meta.yml new file mode 100644 index 00000000..8ba07350 --- /dev/null +++ b/modules/nf-core/tidk/search/meta.yml @@ -0,0 +1,51 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "tidk_search" +description: Searches a genome for a telomere string such as TTAGGG +keywords: + - genomics + - telomere + - search +tools: + - "tidk": + description: tidk is a toolkit to identify and visualise telomeric repeats in genomes + homepage: "https://github.com/tolkit/telomeric-identifier" + documentation: "https://github.com/tolkit/telomeric-identifier" + tool_dev_url: "https://github.com/tolkit/telomeric-identifier" + doi: "10.5281/zenodo.10091385" + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fasta: + type: file + description: The input fasta file + pattern: "*.{fsa,fa,fasta}" + - string: + type: string + description: Search string such as TTAGGG +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - tsv: + type: file + description: Search results in TSV format + pattern: "*.tsv" + - bedgraph: + type: file + description: Search results in BEDGRAPH format + pattern: "*.bedgraph" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/nf-core/tidk/search/tests/main.nf.test b/modules/nf-core/tidk/search/tests/main.nf.test new file mode 100644 index 00000000..64e1d3a5 --- /dev/null +++ b/modules/nf-core/tidk/search/tests/main.nf.test @@ -0,0 +1,119 @@ +nextflow_process { + + name "Test Process TIDK_SEARCH" + script "../main.nf" + process "TIDK_SEARCH" + + tag "modules" + tag "modules_nfcore" + tag "tidk" + tag "tidk/search" + + test("homo_sapiens-genome_fasta-bedgraph") { + + config "./nextflow.config" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + input[1] = 'TTAGGG' + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.bedgraph).match("bedgraph") }, + { assert snapshot(process.out.versions).match("versions") }, + { assert process.out.tsv == [] } + ) + } + + } + + test("homo_sapiens-genome_fasta-tsv") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + input[1] = 'TTAGGG' + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.tsv).match("tsv") }, + { assert snapshot(process.out.versions).match("versions") }, + { assert process.out.bedgraph == [] } + ) + } + + } + + test("stub-bedgraph") { + + options '-stub' + config "./nextflow.config" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + input[1] = 'TTAGGG' + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert process.out.bedgraph != null }, + { assert process.out.tsv == [] }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("stub-tsv") { + + options '-stub' + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + input[1] = 'TTAGGG' + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert process.out.bedgraph == [] }, + { assert process.out.tsv != null }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + +} \ No newline at end of file diff --git a/modules/nf-core/tidk/search/tests/main.nf.test.snap b/modules/nf-core/tidk/search/tests/main.nf.test.snap new file mode 100644 index 00000000..15aedd25 --- /dev/null +++ b/modules/nf-core/tidk/search/tests/main.nf.test.snap @@ -0,0 +1,36 @@ +{ + "bedgraph": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.bedgraph:md5,8277cc74bf083f51584ba6d2b8a8013f" + ] + ] + ], + "timestamp": "2023-12-05T09:53:52.893139" + }, + "versions": { + "content": [ + [ + "versions.yml:md5,6baeeb0e50c9bea6975173a329179f8e" + ] + ], + "timestamp": "2023-12-05T09:53:52.89726" + }, + "tsv": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.tsv:md5,5b44a89396f412eb571ea240ef9deedd" + ] + ] + ], + "timestamp": "2023-12-05T09:53:56.455271" + } +} \ No newline at end of file diff --git a/modules/nf-core/tidk/search/tests/nextflow.config b/modules/nf-core/tidk/search/tests/nextflow.config new file mode 100644 index 00000000..5d36acfb --- /dev/null +++ b/modules/nf-core/tidk/search/tests/nextflow.config @@ -0,0 +1,3 @@ +process { + ext.args = '--extension bedgraph' +} diff --git a/modules/nf-core/tidk/search/tests/tags.yml b/modules/nf-core/tidk/search/tests/tags.yml new file mode 100644 index 00000000..6d27fc6d --- /dev/null +++ b/modules/nf-core/tidk/search/tests/tags.yml @@ -0,0 +1,2 @@ +tidk/search: + - "modules/nf-core/tidk/search/**" diff --git a/subworkflows/nf-core/fasta_explore_search_plot_tidk/main.nf b/subworkflows/nf-core/fasta_explore_search_plot_tidk/main.nf new file mode 100644 index 00000000..9dc3e6cc --- /dev/null +++ b/subworkflows/nf-core/fasta_explore_search_plot_tidk/main.nf @@ -0,0 +1,90 @@ +include { SEQKIT_SEQ as FILTER_BY_LENGTH } from '../../../modules/nf-core/seqkit/seq/main' +include { SEQKIT_SORT as SORT_BY_LENGTH } from '../../../modules/nf-core/seqkit/sort/main' +include { TIDK_EXPLORE } from '../../../modules/nf-core/tidk/explore/main' +include { TIDK_SEARCH as TIDK_SEARCH_APRIORI } from '../../../modules/nf-core/tidk/search/main' +include { TIDK_SEARCH as TIDK_SEARCH_APOSTERIORI } from '../../../modules/nf-core/tidk/search/main' +include { TIDK_PLOT as TIDK_PLOT_APRIORI } from '../../../modules/nf-core/tidk/plot/main' +include { TIDK_PLOT as TIDK_PLOT_APOSTERIORI } from '../../../modules/nf-core/tidk/plot/main' + + +workflow FASTA_EXPLORE_SEARCH_PLOT_TIDK { + + take: + ch_fasta // channel: [ val(meta), [ fasta ] ] + ch_apriori_sequence // channel: [ val(meta), val(sequence) ]; Optional: Set to [] if not needed + // val(meta) from ch_fasta and ch_apriori_sequence are only required to have + // the same `id` + + main: + ch_versions = Channel.empty() + + // MODULE: SEQKIT_SEQ as FILTER_BY_LENGTH + FILTER_BY_LENGTH ( ch_fasta ) + + ch_filtered_fasta = FILTER_BY_LENGTH.out.fastx + ch_versions = ch_versions.mix(FILTER_BY_LENGTH.out.versions.first()) + + // MODULE: SEQKIT_SORT as SORT_BY_LENGTH + SORT_BY_LENGTH ( ch_filtered_fasta ) + + ch_sorted_fasta = SORT_BY_LENGTH.out.fastx + ch_versions = ch_versions.mix(SORT_BY_LENGTH.out.versions.first()) + + // TIDK_EXPLORE + TIDK_EXPLORE ( ch_filtered_fasta ) + + ch_top_sequence = TIDK_EXPLORE.out.top_sequence + ch_versions = ch_versions.mix(TIDK_EXPLORE.out.versions.first()) + + // TIDK_SEARCH as TIDK_SEARCH_APRIORI + ch_apriori_inputs = ch_sorted_fasta + | map { meta, fasta -> [ meta.id, meta, fasta ] } + | join( + ( ch_apriori_sequence ?: Channel.empty() ) + | map { meta, seq -> [ meta.id, seq ] } + ) + | map { id, meta, fasta, seq -> [ meta, fasta, seq ] } + + TIDK_SEARCH_APRIORI ( + ch_apriori_inputs.map { meta, fasta, seq -> [ meta, fasta ] }, + ch_apriori_inputs.map { meta, fasta, seq -> seq } + ) + + ch_apriori_tsv = TIDK_SEARCH_APRIORI.out.tsv + ch_versions = ch_versions.mix(TIDK_SEARCH_APRIORI.out.versions.first()) + + // TIDK_SEARCH as TIDK_SEARCH_APOSTERIORI + ch_aposteriori_inputs = ch_sorted_fasta + | join(ch_top_sequence) + | map { meta, fasta, txt -> + [ meta, fasta, txt.getText().strip() ] + } + + TIDK_SEARCH_APOSTERIORI ( + ch_aposteriori_inputs.map { meta, fasta, seq -> [ meta, fasta ] }, + ch_aposteriori_inputs.map { meta, fasta, seq -> seq } + ) + + ch_aposteriori_tsv = TIDK_SEARCH_APOSTERIORI.out.tsv + ch_versions = ch_versions.mix(TIDK_SEARCH_APOSTERIORI.out.versions.first()) + + // TIDK_PLOT as TIDK_PLOT_APRIORI + TIDK_PLOT_APRIORI ( ch_apriori_tsv ) + + ch_apriori_svg = TIDK_PLOT_APRIORI.out.svg + ch_versions = ch_versions.mix(TIDK_PLOT_APRIORI.out.versions.first()) + + // TIDK_PLOT as TIDK_PLOT_APOSTERIORI + TIDK_PLOT_APOSTERIORI ( ch_aposteriori_tsv ) + + ch_aposteriori_svg = TIDK_PLOT_APOSTERIORI.out.svg + ch_versions = ch_versions.mix(TIDK_PLOT_APOSTERIORI.out.versions.first()) + + emit: + apriori_tsv = ch_apriori_tsv // channel: [ val(meta), tsv ] + apriori_svg = ch_apriori_svg // channel: [ val(meta), svg ] + aposteriori_sequence = ch_top_sequence // channel: [ val(meta), txt ] + aposteriori_tsv = ch_aposteriori_tsv // channel: [ val(meta), tsv ] + aposteriori_svg = ch_aposteriori_svg // channel: [ val(meta), svg ] + versions = ch_versions // channel: [ versions.yml ] +} diff --git a/subworkflows/nf-core/fasta_explore_search_plot_tidk/meta.yml b/subworkflows/nf-core/fasta_explore_search_plot_tidk/meta.yml new file mode 100644 index 00000000..80bd15fd --- /dev/null +++ b/subworkflows/nf-core/fasta_explore_search_plot_tidk/meta.yml @@ -0,0 +1,74 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "fasta_explore_search_plot_tidk" +description: | + Uses Telomere Identification toolKit (TIDK) to identify the frequency of telomeric repeats + along a sliding window for each sequence in the input fasta file. Results are presented in + TSV and SVG formats. The user can specify an a priori sequence for identification. + Possible a posteriori sequences are also explored and the most frequent sequence is + used for identification similar to the a priori sequence. seqkit/seq and seqkit/sort modules are + also included to filter out small sequences and sort sequences by length. +keywords: + - genomics + - telomere + - repeat + - search + - plot +components: + - seqkit/seq + - seqkit/sort + - tidk/explore + - tidk/plot + - tidk/search +input: + - ch_fasta: + type: file + description: | + Input assembly + Structure: [ val(meta), path(fasta) ] + pattern: "*.{fsa/fa/fasta}" + - ch_apriori_sequence: + type: string + description: | + A priori sequence + Structure: [ val(meta), val(sequence) ] +output: + - apriori_tsv: + type: file + description: | + Frequency table for the identification of the a priori sequence + Structure: [ val(meta), path(tsv) ] + pattern: "*.tsv" + - apriori_svg: + type: file + description: | + Frequency graph for the identification of the a priori sequence + Structure: [ val(meta), path(svg) ] + pattern: "*.svg" + - aposteriori_sequence: + type: file + description: | + The most frequent a posteriori sequence + Structure: [ val(meta), path(txt) ] + pattern: "*.txt" + - aposteriori_tsv: + type: file + description: | + Frequency table for the identification of the a aposteriori sequence + Structure: [ val(meta), path(tsv) ] + pattern: "*.tsv" + - aposteriori_svg: + type: file + description: | + Frequency graph for the identification of the a aposteriori sequence + Structure: [ val(meta), path(svg) ] + pattern: "*.svg" + - versions: + type: file + description: | + File containing software versions + Structure: [ path(versions.yml) ] + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test b/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test new file mode 100644 index 00000000..9f69267d --- /dev/null +++ b/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test @@ -0,0 +1,119 @@ +nextflow_workflow { + + name "Test Workflow FASTA_EXPLORE_SEARCH_PLOT_TIDK" + script "../main.nf" + workflow "FASTA_EXPLORE_SEARCH_PLOT_TIDK" + config './nextflow.config' + + tag "subworkflows" + tag "subworkflows_nfcore" + tag "subworkflows/fasta_explore_search_plot_tidk" + tag "fasta_explore_search_plot_tidk" + tag "seqkit/seq" + tag "seqkit/sort" + tag "tidk/explore" + tag "tidk/search" + tag "tidk/plot" + + test("homo_sapiens-genome_fasta-genome_21_fasta") { + + when { + workflow { + """ + input[0] = Channel.of( + [ + [ id:'test' ], + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ], + [ + [ id:'test2' ], + file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) + ] + ) + input[1] = Channel.of( + [ + [ id:'test' ], + 'TTAGGG' + ], + [ + [ id:'test2' ], + 'TTAGGG' + ] + ) + """ + } + } + + then { + assertAll( + { assert workflow.success}, + { assert snapshot(workflow.out).match()} + ) + } + } + + test("homo_sapiens-genome_fasta-genome_21_fasta-partial_apriori-stub") { + + options '-stub' + + when { + workflow { + """ + input[0] = Channel.of( + [ + [ id:'test' ], + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ], + [ + [ id:'test2' ], + file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) + ] + ) + input[1] = Channel.of( + [ + [ id:'test' ], + 'TTAGGG' + ] + ) + """ + } + } + + then { + assertAll( + { assert workflow.success}, + { assert snapshot(workflow.out).match()} + ) + } + } + + test("homo_sapiens-genome_fasta-genome_21_fasta-no_apriori-stub") { + + options '-stub' + + when { + workflow { + """ + input[0] = Channel.of( + [ + [ id:'test' ], + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ], + [ + [ id:'test2' ], + file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) + ] + ) + input[1] = [] + """ + } + } + + then { + assertAll( + { assert workflow.success}, + { assert snapshot(workflow.out).match()} + ) + } + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test.snap b/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test.snap new file mode 100644 index 00000000..6e4e99c6 --- /dev/null +++ b/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test.snap @@ -0,0 +1,389 @@ +{ + "homo_sapiens-genome_fasta-genome_21_fasta": { + "content": [ + { + "0": [ + [ + { + "id": "test2" + }, + "test2.tsv:md5,4a42ed016c022a8238739e4acddf649c" + ], + [ + { + "id": "test" + }, + "test.tsv:md5,5b44a89396f412eb571ea240ef9deedd" + ] + ], + "1": [ + [ + { + "id": "test2" + }, + "test2.svg:md5,e76985fdc220867a05967984c2c4675d" + ], + [ + { + "id": "test" + }, + "test.svg:md5,d1bca1077cd52ad8c5126a4c9614d8f7" + ] + ], + "2": [ + [ + { + "id": "test2" + }, + "test2.top.sequence.txt:md5,bd63900958df06516e45b887072d788f" + ] + ], + "3": [ + [ + { + "id": "test2" + }, + "test2.tsv:md5,43f358de532d30e02e65339db07e4e00" + ] + ], + "4": [ + [ + { + "id": "test2" + }, + "test2.svg:md5,f0a6d4bb1464125bcbb6b54a471c1e3c" + ] + ], + "5": [ + "versions.yml:md5,02d48eb43c3882d9832c88b9e080b420", + "versions.yml:md5,506585e66b23f17620bf582ef60af56d", + "versions.yml:md5,5f9958ea613eceae4b09bb42ecaac656", + "versions.yml:md5,6713d71b01fe0402e5d32f1a5a181e0f", + "versions.yml:md5,83a6abbf9b68ec1e6152c5b6eb12f0d7", + "versions.yml:md5,b900c2a21c17ac2d16e33641e64fc7c1", + "versions.yml:md5,d0a1f1950f8dc5eb106b04a9364c8f04" + ], + "aposteriori_sequence": [ + [ + { + "id": "test2" + }, + "test2.top.sequence.txt:md5,bd63900958df06516e45b887072d788f" + ] + ], + "aposteriori_svg": [ + [ + { + "id": "test2" + }, + "test2.svg:md5,f0a6d4bb1464125bcbb6b54a471c1e3c" + ] + ], + "aposteriori_tsv": [ + [ + { + "id": "test2" + }, + "test2.tsv:md5,43f358de532d30e02e65339db07e4e00" + ] + ], + "apriori_svg": [ + [ + { + "id": "test2" + }, + "test2.svg:md5,e76985fdc220867a05967984c2c4675d" + ], + [ + { + "id": "test" + }, + "test.svg:md5,d1bca1077cd52ad8c5126a4c9614d8f7" + ] + ], + "apriori_tsv": [ + [ + { + "id": "test2" + }, + "test2.tsv:md5,4a42ed016c022a8238739e4acddf649c" + ], + [ + { + "id": "test" + }, + "test.tsv:md5,5b44a89396f412eb571ea240ef9deedd" + ] + ], + "versions": [ + "versions.yml:md5,02d48eb43c3882d9832c88b9e080b420", + "versions.yml:md5,506585e66b23f17620bf582ef60af56d", + "versions.yml:md5,5f9958ea613eceae4b09bb42ecaac656", + "versions.yml:md5,6713d71b01fe0402e5d32f1a5a181e0f", + "versions.yml:md5,83a6abbf9b68ec1e6152c5b6eb12f0d7", + "versions.yml:md5,b900c2a21c17ac2d16e33641e64fc7c1", + "versions.yml:md5,d0a1f1950f8dc5eb106b04a9364c8f04" + ] + } + ], + "timestamp": "2023-12-12T15:33:35.106116" + }, + 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+ } + + withName: SORT_BY_LENGTH { + ext.args = '--quiet --reverse --by-length' + ext.prefix = { "${meta.id}.sorted" } + } + + withName: TIDK_EXPLORE { + ext.args = '--minimum 5 --maximum 30' + } + + withName: 'TIDK_SEARCH_*' { + ext.args = '--extension tsv' + } +} diff --git a/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/tags.yml b/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/tags.yml new file mode 100644 index 00000000..d2d150d7 --- /dev/null +++ b/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/fasta_explore_search_plot_tidk: + - subworkflows/nf-core/fasta_explore_search_plot_tidk/** diff --git a/workflows/assembly_qc.nf b/workflows/assemblyqc.nf similarity index 66% rename from workflows/assembly_qc.nf rename to workflows/assemblyqc.nf index 8ef70d26..1284619e 100644 --- a/workflows/assembly_qc.nf +++ b/workflows/assemblyqc.nf @@ -1,29 +1,30 @@ nextflow.enable.dsl=2 -include { validateParams } from '../modules/local/utils.nf' -include { jsonifyParams } from '../modules/local/utils.nf' - -include { VALIDATE_FASTA } from '../subworkflows/local/validate_fasta.nf' -include { VALIDATE_GFF3 } from '../subworkflows/local/validate_gff3.nf' -include { BUSCO } from '../subworkflows/local/busco.nf' -include { TIDK } from '../subworkflows/local/tidk.nf' -include { FASTA_LTRRETRIEVER_LAI } from '../subworkflows/pfr/fasta_ltrretriever_lai/main.nf' -include { KRAKEN2 } from '../subworkflows/local/kraken2.nf' -include { NCBI_FCS_ADAPTOR } from '../subworkflows/local/ncbi_fcs_adaptor.nf' -include { NCBI_FCS_GX } from '../subworkflows/local/ncbi_fcs_gx.nf' -include { FASTQ_TRIM_FASTP_FASTQC } from '../subworkflows/nf-core/fastq_trim_fastp_fastqc/main' -include { HIC_CONTACT_MAP } from '../subworkflows/local/hic_contact_map.nf' -include { SYNTENY } from '../subworkflows/local/synteny.nf' - -include { CREATE_REPORT } from '../modules/local/create_report.nf' -include { ASSEMBLATHON_STATS } from '../modules/local/assemblathon_stats.nf' -include { GENOMETOOLS_GT_STAT } from '../modules/local/genometools_gt_stat.nf' -include { BIOCODE_GFF3_STATS } from '../modules/local/biocode_gff3_stats.nf' +include { validateParams } from '../modules/local/utils' +include { jsonifyParams } from '../modules/local/utils' + +include { VALIDATE_FASTA } from '../subworkflows/local/validate_fasta' +include { VALIDATE_GFF3 } from '../subworkflows/local/validate_gff3' +include { BUSCO } from '../subworkflows/local/busco' +include { FASTA_EXPLORE_SEARCH_PLOT_TIDK } from '../subworkflows/nf-core/fasta_explore_search_plot_tidk/main' +include { FASTA_LTRRETRIEVER_LAI } from '../subworkflows/pfr/fasta_ltrretriever_lai/main' +include { KRAKEN2 } from '../subworkflows/local/kraken2' +include { NCBI_FCS_ADAPTOR } from '../subworkflows/local/ncbi_fcs_adaptor' +include { NCBI_FCS_GX } from '../subworkflows/local/ncbi_fcs_gx' +include { FASTQ_TRIM_FASTP_FASTQC } from '../subworkflows/nf-core/fastq_trim_fastp_fastqc/main' +include { HIC_CONTACT_MAP } from '../subworkflows/local/hic_contact_map' +include { SYNTENY } from '../subworkflows/local/synteny' + +include { CREATE_REPORT } from '../modules/local/create_report' +include { ASSEMBLATHON_STATS } from '../modules/local/assemblathon_stats' +include { GENOMETOOLS_GT_STAT } from '../modules/local/genometools_gt_stat' +include { BIOCODE_GFF3_STATS } from '../modules/local/biocode_gff3_stats' + validateParams(params) def paramsAsJSON = jsonifyParams(params) -workflow ASSEMBLY_QC { +workflow ASSEMBLYQC { // VALIDATE_FASTA Channel.fromList(params.target_assemblies) @@ -102,7 +103,28 @@ workflow ASSEMBLY_QC { | BUSCO // TIDK - TIDK(ch_clean_target_assemblies) + ch_tidk_inputs = params.tidk.skip + ? Channel.empty() + : ch_clean_target_assemblies + | map { tag, fa -> [ [ id: tag ], fa ] } + | combine( + Channel.of(params.tidk.repeat_seq) + ) + + FASTA_EXPLORE_SEARCH_PLOT_TIDK( + ch_tidk_inputs.map { meta, fa, seq -> [ meta, fa ] }, + ch_tidk_inputs.map { meta, fa, seq -> [ meta, seq ] } + ) + + ch_tidk_outputs = FASTA_EXPLORE_SEARCH_PLOT_TIDK.out.apriori_svg + | mix(FASTA_EXPLORE_SEARCH_PLOT_TIDK.out.aposteriori_svg) + | mix(FASTA_EXPLORE_SEARCH_PLOT_TIDK.out.aposteriori_sequence) + | map { meta, file -> file } + | mix( + Channel.of("$params.tidk.repeat_seq") + | collectFile(name: 'a_priori.sequence', newLine: true) + ) + | collect // FASTA_LTRRETRIEVER_LAI ch_lai_inputs = params.lai.skip @@ -194,7 +216,7 @@ workflow ASSEMBLY_QC { ch_genometools_gt_stats.ifEmpty([]), ch_biocode_gff3_stats.ifEmpty([]), BUSCO.out.list_of_outputs.ifEmpty([]), - TIDK.out.list_of_plots.ifEmpty([]), + ch_tidk_outputs.ifEmpty([]), ch_lai_outputs.ifEmpty([]), KRAKEN2.out.list_of_outputs.ifEmpty([]), HIC_CONTACT_MAP.out.list_of_html_files.ifEmpty([]), From aa80bbb74a74f402b1c312fd053454863eb8d309 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 19 Feb 2024 20:37:13 +1300 Subject: [PATCH 05/65] Multiple changes, see CHANGELOG.md --- CHANGELOG.md | 14 +- CITATIONS.md | 147 ++-- README.md | 4 +- bin/assembly_qc_report_943e0fb.py | 46 +- bin/check_gff3_fasta_corresp_3031aca.sh | 54 -- .../parsers/biocode_gff3_stats_parser.py | 214 ----- .../parsers/fasta_validate_parser.py | 35 + .../parsers/genometools_gt_stat_parser.py | 312 ++++++- .../parsers/gff3_validate_parser.py | 35 + bin/report_modules/parsers/tools_parser.py | 33 + .../assemblathon_stats.html | 4 +- bin/report_modules/templates/base.html | 41 +- .../biocode_gff3_stats.html | 13 - .../biocode_gff3_stats/dropdown.html | 10 - .../biocode_gff3_stats/report_contents.html | 20 - bin/report_modules/templates/busco/busco.html | 4 +- .../templates/circos/circos.html | 10 +- .../templates/fasta_validate/dropdown.html | 10 + .../fasta_validate/fasta_validate.html | 14 + .../fasta_validate/report_contents.html | 18 + .../genometools_gt_stat.html | 2 +- .../genometools_gt_stat/report_contents.html | 18 + .../templates/gff3_validate/dropdown.html | 10 + .../gff3_validate/gff3_validate.html | 22 + .../gff3_validate/report_contents.html | 18 + bin/report_modules/templates/hic/hic.html | 7 +- .../templates/kraken2/kraken2.html | 6 +- bin/report_modules/templates/lai/lai.html | 4 +- .../ncbi_fcs_adaptor/ncbi_fcs_adaptor.html | 2 +- .../templates/ncbi_fcs_gx/ncbi_fcs_gx.html | 7 +- .../templates/params/params.html | 2 +- bin/report_modules/templates/tidk/tidk.html | 2 +- .../templates/tools/contents.html | 9 + bin/report_modules/templates/tools/tools.html | 1 + bin/reverse_edta_naming_f1b7bce.py | 48 -- bin/shorten_fasta_ids_c97537f.py | 170 ---- conf/base.config | 4 + conf/modules.config | 353 ++++---- conf/test_invalid.config | 40 + modules.json | 40 + modules/local/agp2_assembly.nf | 14 +- modules/local/assemblathon_stats.nf | 51 +- modules/local/assembly2_bedpe.nf | 2 - modules/local/biocode_gff3_stats.nf | 22 - modules/local/create_report.nf | 44 +- modules/local/genometools_gt_stat.nf | 24 - modules/local/gzip_fasta.nf | 24 - modules/local/hic_qc.nf | 2 - modules/local/juicer_sort.nf | 16 +- modules/local/make_agp_from_fasta.nf | 2 - modules/local/matlock_bam2_juicer.nf | 2 - modules/local/ncbi_fcs_adaptor.nf | 34 + modules/local/run_assembly_visualizer.nf | 23 +- modules/local/utils.nf | 2 - .../dumpsoftwareversions/environment.yml | 7 + .../custom/dumpsoftwareversions/main.nf | 24 + .../custom/dumpsoftwareversions/meta.yml | 37 + .../templates/dumpsoftwareversions.py | 103 +++ .../dumpsoftwareversions/tests/main.nf.test | 43 + .../tests/main.nf.test.snap | 33 + .../dumpsoftwareversions/tests/tags.yml | 2 + .../nf-core/fastavalidator/environment.yml | 9 + modules/nf-core/fastavalidator/main.nf | 62 ++ modules/nf-core/fastavalidator/meta.yml | 53 ++ .../nf-core/fastavalidator/tests/main.nf.test | 60 ++ .../fastavalidator/tests/main.nf.test.snap | 76 ++ modules/nf-core/fastavalidator/tests/tags.yml | 2 + modules/nf-core/gunzip/environment.yml | 7 + modules/nf-core/gunzip/main.nf | 48 ++ modules/nf-core/gunzip/meta.yml | 39 + modules/nf-core/gunzip/tests/main.nf.test | 36 + .../nf-core/gunzip/tests/main.nf.test.snap | 31 + modules/nf-core/gunzip/tests/tags.yml | 2 + .../environment.yml | 9 + .../checkgff3fastacorrespondence/main.nf | 25 + .../checkgff3fastacorrespondence/meta.yml | 56 ++ .../check_gff3_fasta_correspondence.sh | 70 ++ .../tests/main.nf.test | 70 ++ .../tests/main.nf.test.snap | 72 ++ .../tests/tags.yml | 2 + modules/pfr/gt/gff3/environment.yml | 9 + modules/pfr/gt/gff3/main.nf | 51 ++ modules/pfr/gt/gff3/meta.yml | 48 ++ modules/pfr/gt/gff3/tests/main.nf.test | 61 ++ modules/pfr/gt/gff3/tests/main.nf.test.snap | 72 ++ modules/pfr/gt/gff3/tests/nextflow.config | 3 + modules/pfr/gt/gff3/tests/tags.yml | 2 + modules/pfr/gt/gff3validator/environment.yml | 9 + modules/pfr/gt/gff3validator/main.nf | 50 ++ modules/pfr/gt/gff3validator/meta.yml | 49 ++ .../pfr/gt/gff3validator/tests/main.nf.test | 63 ++ .../gt/gff3validator/tests/main.nf.test.snap | 72 ++ modules/pfr/gt/gff3validator/tests/tags.yml | 2 + modules/pfr/gt/stat/environment.yml | 9 + modules/pfr/gt/stat/main.nf | 35 + modules/pfr/gt/stat/meta.yml | 46 ++ modules/pfr/gt/stat/tests/main.nf.test | 37 + modules/pfr/gt/stat/tests/main.nf.test.snap | 31 + modules/pfr/gt/stat/tests/tags.yml | 2 + nextflow.config | 1 + subworkflows/local/busco.nf | 90 +-- subworkflows/local/create_hic_file.nf | 31 +- subworkflows/local/hic_contact_map.nf | 75 +- subworkflows/local/kraken2.nf | 111 ++- subworkflows/local/ncbi_fcs_adaptor.nf | 107 --- subworkflows/local/ncbi_fcs_gx.nf | 203 ++--- subworkflows/local/synteny.nf | 761 +++++++++--------- subworkflows/local/tidk.nf | 212 ----- subworkflows/local/validate_fasta.nf | 52 -- subworkflows/local/validate_gff3.nf | 143 ---- subworkflows/pfr/gff3_validate/main.nf | 61 ++ subworkflows/pfr/gff3_validate/meta.yml | 50 ++ .../pfr/gff3_validate/tests/main.nf.test | 86 ++ .../pfr/gff3_validate/tests/main.nf.test.snap | 115 +++ .../pfr/gff3_validate/tests/nextflow.config | 6 + subworkflows/pfr/gff3_validate/tests/tags.yml | 2 + version_check.sh | 10 +- workflows/assemblyqc.nf | 475 +++++++---- 118 files changed, 3961 insertions(+), 2324 deletions(-) delete mode 100755 bin/check_gff3_fasta_corresp_3031aca.sh delete mode 100644 bin/report_modules/parsers/biocode_gff3_stats_parser.py create mode 100644 bin/report_modules/parsers/fasta_validate_parser.py create mode 100644 bin/report_modules/parsers/gff3_validate_parser.py create mode 100644 bin/report_modules/parsers/tools_parser.py delete mode 100644 bin/report_modules/templates/biocode_gff3_stats/biocode_gff3_stats.html delete mode 100644 bin/report_modules/templates/biocode_gff3_stats/dropdown.html delete mode 100644 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mode 100644 modules/pfr/gt/gff3/environment.yml create mode 100644 modules/pfr/gt/gff3/main.nf create mode 100644 modules/pfr/gt/gff3/meta.yml create mode 100644 modules/pfr/gt/gff3/tests/main.nf.test create mode 100644 modules/pfr/gt/gff3/tests/main.nf.test.snap create mode 100644 modules/pfr/gt/gff3/tests/nextflow.config create mode 100644 modules/pfr/gt/gff3/tests/tags.yml create mode 100644 modules/pfr/gt/gff3validator/environment.yml create mode 100644 modules/pfr/gt/gff3validator/main.nf create mode 100644 modules/pfr/gt/gff3validator/meta.yml create mode 100644 modules/pfr/gt/gff3validator/tests/main.nf.test create mode 100644 modules/pfr/gt/gff3validator/tests/main.nf.test.snap create mode 100644 modules/pfr/gt/gff3validator/tests/tags.yml create mode 100644 modules/pfr/gt/stat/environment.yml create mode 100644 modules/pfr/gt/stat/main.nf create mode 100644 modules/pfr/gt/stat/meta.yml create mode 100644 modules/pfr/gt/stat/tests/main.nf.test create mode 100644 modules/pfr/gt/stat/tests/main.nf.test.snap create mode 100644 modules/pfr/gt/stat/tests/tags.yml delete mode 100644 subworkflows/local/ncbi_fcs_adaptor.nf delete mode 100644 subworkflows/local/tidk.nf delete mode 100644 subworkflows/local/validate_fasta.nf delete mode 100644 subworkflows/local/validate_gff3.nf create mode 100644 subworkflows/pfr/gff3_validate/main.nf create mode 100644 subworkflows/pfr/gff3_validate/meta.yml create mode 100644 subworkflows/pfr/gff3_validate/tests/main.nf.test create mode 100644 subworkflows/pfr/gff3_validate/tests/main.nf.test.snap create mode 100644 subworkflows/pfr/gff3_validate/tests/nextflow.config create mode 100644 subworkflows/pfr/gff3_validate/tests/tags.yml diff --git a/CHANGELOG.md b/CHANGELOG.md index 36170a52..03dd8732 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -1,10 +1,22 @@ # Change Log -## Version 1.4 (14-Feb-2023) +## Version 1.4 (20-Feb-2023) 1. Now it is possible to skip FASTP and FASTQC for the HIC module 2. Renamed ASSEMBLY_QC workflow to ASSEMBLYQC 3. Now using nf-core/FASTA_EXPLORE_SEARCH_PLOT_TIDK +4. Now redirecting validation errors to AssemblyQC report +5. Simplified layout of CITATIONS.md file +6. Now using pfr/gff3_validate sub-workflow for gff3 validation +7. Now listing software versions from the versions.yml file +8. Replaced custom GUNZIP module with nf-core/gunzip +9. Replaced custom gt/stat with pfr/gt/stat +10. Replaced custom fasta_validator with nf-core/fastavalidator +11. Removed BIOCODE GFF3 STATS owing to its frequent failures +12. Added pre-commit version checking +13. Now gt/stat reports extended stats and multiple distribution plots have been added to the report +14. Added a tools tab to the report which lists the tools used by the pipeline to create the report +15. Refactored and cleaned data flows for all the custom sub-workflow ## Version 1.3 (08-Feb-2023) diff --git a/CITATIONS.md b/CITATIONS.md index 3a80b8e8..19b4531d 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -1,107 +1,106 @@ ## Citations -- nf-core/modules([MIT](https://github.com/nf-core/modules/blob/master/LICENSE)) +- nf-core/modules, [MIT](https://github.com/nf-core/modules/blob/master/LICENSE) > Ewels PA, Peltzer A, Fillinger S et al. 2020. The nf-core framework for community-curated bioinformatics pipelines. Nat Biotechnol 38, 276–278 (2020). doi: -- FASTA_VALIDATE ([MIT](https://github.com/GallVp/fasta_validator/blob/master/LICENSE)) - > - > - > Forked from: - > - > Edwards RA. 2019. fasta_validate: a fast and efficient fasta validator written in pure C. doi: -- GT_GFF3VALIDATOR ([ISC](http://genometools.org/license.html)) +- py_fasta_validator, [MIT](https://github.com/linsalrob/py_fasta_validator/blob/master/LICENSE) - > Gremme G, Steinbiss S, Kurtz S. 2013. "GenomeTools: A Comprehensive Software Library for Efficient Processing of Structured Genome Annotations," in IEEE/ACM Transactions on Computational Biology and Bioinformatics, vol. 10, no. 3, pp. 645-656, May 2013, doi: . + > Edwards, R.A. 2019. fasta_validate: a fast and efficient fasta validator written in pure C. doi: - GT_GFF3VALIDATOR workflow also employs: +- GenomeTools, [ISC](http://genometools.org/license.html) - - SAMTOOLS (1.16.1, [MIT/Expat](https://github.com/samtools/samtools/blob/develop/LICENSE)) - > Danecek P, Bonfield JK, Liddle J, Marshall J, Ohan V, Pollard MO, Whitwham A, Keane T, McCarthy SA, Davies RM, Li H. 2021. Twelve years of SAMtools and BCFtools, GigaScience, Volume 10, Issue 2, February 2021, giab008, + > Gremme G, Steinbiss S, Kurtz S. 2013. "GenomeTools: A Comprehensive Software Library for Efficient Processing of Structured Genome Annotations," in IEEE/ACM Transactions on Computational Biology and Bioinformatics, vol. 10, no. 3, pp. 645-656, May 2013, doi: -- NCBI-FCS-ADAPTOR (0.4, [License](https://github.com/ncbi/fcs/blob/main/LICENSE.txt)) - > -- NCBI-FCS-GX (0.4, [License](https://github.com/ncbi/fcs/blob/main/LICENSE.txt)) +- SAMTOOLS, [MIT/Expat](https://github.com/samtools/samtools/blob/develop/LICENSE) - > - > - > Astashyn A, Tvedte ES, Sweeney D, Sapojnikov V, Bouk N, Joukov V, Mozes E, Strope PK, Sylla PM, Wagner L, Bidwell SL, Clark K, Davis EW, Smith-White B, Hlavina W, Pruitt KD, Schneider VA, Murphy TD. 2023. bioRxiv 2023.06.02.543519; doi: + > Danecek P, Bonfield JK, Liddle J, Marshall J, Ohan V, Pollard MO, Whitwham A, Keane T, McCarthy SA, Davies RM, Li H. 2021. Twelve years of SAMtools and BCFtools, GigaScience, Volume 10, Issue 2, February 2021, giab008, + +- NCBI/FCS, [License](https://github.com/ncbi/fcs/blob/main/LICENSE.txt) - NCBI-FCS-GX workflow also employs: + > Astashyn A, Tvedte ES, Sweeney D, Sapojnikov V, Bouk N, Joukov V, Mozes E, Strope PK, Sylla PM, Wagner L, Bidwell SL, Clark K, Davis EW, Smith-White B, Hlavina W, Pruitt KD, Schneider VA, Murphy TD. 2023. Rapid and sensitive detection of genome contamination at scale with FCS-GX. bioRxiv 2023.06.02.543519; doi: - - KRONA (2.7.1, [License](https://github.com/marbl/Krona/blob/master/KronaTools/LICENSE.txt)) - > Ondov BD, Bergman NH, Phillippy AM. 2011. Interactive metagenomic visualization in a Web browser. BMC Bioinformatics. 2011 Sep 30;12:385. doi: +- KRONA, [License](https://github.com/marbl/Krona/blob/master/KronaTools/LICENSE.txt) + + > Ondov BD, Bergman NH, Phillippy AM. 2011. Interactive metagenomic visualization in a Web browser. BMC Bioinformatics. 2011 Sep 30;12:385. doi: + +- assemblathon_stats, [CC BY-NC-SA 3.0](https://creativecommons.org/licenses/by-nc-sa/3.0/) -- ASSEMBLATHON_STATS ([CC BY-NC-SA 3.0](https://creativecommons.org/licenses/by-nc-sa/3.0/)) > [github/PlantandFoodResearch/assemblathon2-analysis/a93cba2](https://github.com/PlantandFoodResearch/assemblathon2-analysis/blob/a93cba25d847434f7eadc04e63b58c567c46a56d/assemblathon_stats.pl) > > Forked from: -- GENOMETOOLS_GT_STAT (1.6.2, [ISC](http://genometools.org/license.html)): - > Gremme G, Steinbiss S, Kurtz S. 2013. "GenomeTools: A Comprehensive Software Library for Efficient Processing of Structured Genome Annotations," in IEEE/ACM Transactions on Computational Biology and Bioinformatics, vol. 10, no. 3, pp. 645-656, May 2013, doi: . -- BIOCODE_GFF3_STATS (0.10.0, [MIT](https://github.com/jorvis/biocode/blob/master/LICENSE)) - > -- BUSCO (5.2.2, [MIT](https://gitlab.com/ezlab/busco/-/blob/master/LICENSE)) + +- BUSCO, [MIT](https://gitlab.com/ezlab/busco/-/blob/master/LICENSE) + > Manni M, Berkeley MR, Seppey M, Simão FA, Zdobnov EM. 2021. BUSCO Update: Novel and Streamlined Workflows along with Broader and Deeper Phylogenetic Coverage for Scoring of Eukaryotic, Prokaryotic, and Viral Genomes, Molecular Biology and Evolution, Volume 38, Issue 10, October 2021, Pages 4647–4654, -- TIDK (0.2.31, [MIT](https://github.com/tolkit/telomeric-identifier/blob/main/LICENSE)) + +- TIDK, [MIT](https://github.com/tolkit/telomeric-identifier/blob/main/LICENSE) > - TIDK workflow also employs: +- SEQKIT, [MIT](https://github.com/shenwei356/seqkit/blob/master/LICENSE) - - SEQKIT (2.3.1, [MIT](https://github.com/shenwei356/seqkit/blob/master/LICENSE)) - > Shen W, Le S, Li Y, Hu F. 2016. SeqKit: A Cross-Platform and Ultrafast Toolkit for FASTA/Q File Manipulation. PLoS ONE 11(10): e0163962. + > Shen W, Le S, Li Y, Hu F. 2016. SeqKit: A Cross-Platform and Ultrafast Toolkit for FASTA/Q File Manipulation. PLoS ONE 11(10): e0163962. -- LAI (beta3.2, [GPL v3](https://github.com/oushujun/LTR_retriever/blob/master/LICENSE)) +- LAI, [GPL v3](https://github.com/oushujun/LTR_retriever/blob/master/LICENSE) > Ou S, Chen J, Jiang N. 2018. Assessing genome assembly quality using the LTR Assembly Index (LAI), Nucleic Acids Research, Volume 46, Issue 21, 30 November 2018, Page e126, - LAI workflow also employs: +- LTR_FINDER_parallel, [MIT](https://github.com/oushujun/LTR_FINDER_parallel/blob/master/LICENSE) + + > Ou S, Jiang N 2019. LTR_FINDER_parallel: parallelization of LTR_FINDER enabling rapid identification of long terminal repeat retrotransposons. Mobile DNA 10, 48 (2019). - - LTR_FINDER_parallel (1.2, [MIT](https://github.com/oushujun/LTR_FINDER_parallel/blob/master/LICENSE)) - > Ou S, Jiang N 2019. LTR_FINDER_parallel: parallelization of LTR_FINDER enabling rapid identification of long terminal repeat retrotransposons. Mobile DNA 10, 48 (2019). - - GT_LTRHARVEST (1.6.2, [ISC](http://genometools.org/license.html)) +- LTRharvest, [ISC](http://genometools.org/license.html) - > Gremme G, Steinbiss S, Kurtz S. 2013. "GenomeTools: A Comprehensive Software Library for Efficient Processing of Structured Genome Annotations," in IEEE/ACM Transactions on Computational Biology and Bioinformatics, vol. 10, no. 3, pp. 645-656, May 2013, doi: . + > Ellinghaus, D, Kurtz, S & Willhoeft, U 2008. LTRharvest, an efficient and flexible software for de novo detection of LTR retrotransposons. BMC Bioinformatics 9, 18 (2008). - > Ellinghaus, D, Kurtz, S & Willhoeft, U 2008. LTRharvest, an efficient and flexible software for de novo detection of LTR retrotransposons. BMC Bioinformatics 9, 18 (2008). +- LTR_retriever, [GPL v3](https://github.com/oushujun/LTR_retriever/blob/master/LICENSE) - - LTR_retriever (2.9.0 [GPL v3](https://github.com/oushujun/LTR_retriever/blob/master/LICENSE)) - > Shujun O, Ning J 2018. LTR_retriever: A Highly Accurate and Sensitive Program for Identification of Long Terminal Repeat Retrotransposons, Plant Physiology, 176, 2 (2018). + > Shujun O, Ning J 2018. LTR_retriever: A Highly Accurate and Sensitive Program for Identification of Long Terminal Repeat Retrotransposons, Plant Physiology, 176, 2 (2018). -- KRAKEN2 (2.1.2, [MIT](https://github.com/DerrickWood/kraken2/blob/master/LICENSE)) +- KRAKEN2, [MIT](https://github.com/DerrickWood/kraken2/blob/master/LICENSE) > Wood DE, Salzberg SL, Wood DE, Lu J, Langmead B. 2019. Improved metagenomic analysis with Kraken 2. Genome Biol 20, 257 (2019). - KRAKEN2 workflow also employs: - - - KRONA (2.7.1, [License](https://github.com/marbl/Krona/blob/master/KronaTools/LICENSE.txt)) - > Ondov BD, Bergman NH, Phillippy AM. 2011. Interactive metagenomic visualization in a Web browser. BMC Bioinformatics. 2011 Sep 30;12:385. doi: - -- HIC CONTACT MAP - - JUICEBOX.JS (2.4.3, [MIT](https://github.com/igvteam/juicebox.js/blob/master/LICENSE)) - > Robinson JT, Turner D, Durand NC, Thorvaldsdóttir H, Mesirov JP, Aiden EL. 2018. Juicebox.js Provides a Cloud-Based Visualization System for Hi-C Data. Cell Syst. 2018 Feb 28;6(2):256-258.e1. doi: . Epub 2018 Feb 7. PMID: 29428417; PMCID: PMC6047755. - - FASTP (0.23.4, [MIT](https://github.com/OpenGene/fastp/blob/master/LICENSE)) - > Chen S, Zhou Y, Chen Y, Gu J. 2018. fastp: an ultra-fast all-in-one FASTQ preprocessor, Bioinformatics, Volume 34, Issue 17, 01 September 2018, Pages i884–i890, - - FASTQC (0.12.9, [GPL v3](https://github.com/s-andrews/FastQC/blob/master/LICENSE.txt)) - > - - RUN_ASSEMBLY_VISUALIZER (commit: 63029aa, [MIT](https://github.com/aidenlab/3d-dna/blob/master/LICENSE)) - > Dudchenko O, Batra SS, Omer AD, Nyquist SK, Hoeger M, Durand NC, Shamim MS, Machol I, Lander, Aiden AP, Aiden EL 2017. De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds.Science356, 92-95(2017). doi: . Available at: - - HIC_QC (commit: 6881c33, [AGPL v3](https://github.com/phasegenomics/hic_qc/blob/master/LICENSE)) - > - - JUICEBOX_SCRIPTS (commit: a7ae991, [AGPL v3](https://github.com/phasegenomics/juicebox_scripts/blob/master/LICENSE)) - > - - BWA (0.7.17, [GPL v3](https://github.com/lh3/bwa/blob/master/COPYING)) - > Li H. 2013. Aligning sequence reads, clone sequences and assembly contigs with BWA-MEM. - - MATLOCK ([AGPL v3](https://github.com/phasegenomics/matlock/blob/master/LICENSE)) - > ; - - SAMBLASTER (0.1.26, [MIT](https://github.com/GregoryFaust/samblaster/blob/master/LICENSE.txt)) - > Faust GG, Hall IM. 2014. SAMBLASTER: fast duplicate marking and structural variant read extraction, Bioinformatics, Volume 30, Issue 17, September 2014, Pages 2503–2505, - - SAMTOOLS (1.18, [MIT/Expat](https://github.com/samtools/samtools/blob/develop/LICENSE)) - > Danecek P, Bonfield JK, Liddle J, Marshall J, Ohan V, Pollard MO, Whitwham A, Keane T, McCarthy SA, Davies RM, Li H. 2021. Twelve years of SAMtools and BCFtools, GigaScience, Volume 10, Issue 2, February 2021, giab008, -- SYNTENY - - CIRCOS (0.23-1, [GPL v3](https://www.gnu.org/licenses/gpl-3.0.txt)) - > Krzywinski M, Schein J, Birol I, Connors J, Gascoyne R. Horsman D, ... Marra MA. 2009. Circos: an information aesthetic for comparative genomics. Genome research, 19(9), 1639-1645. - - MUMMER (4.0.0, [Artistic 2.0](https://github.com/mummer4/mummer/blob/master/LICENSE.md)) - > Marçais G, Delcher AL, Phillippy AM, Coston R, Salzberg SL, Zimin A. 2018. MUMmer4: A fast and versatile genome alignment system. PLoS Comput Biol. 2018 Jan 26;14(1):e1005944. doi: . PMID: 29373581; PMCID: PMC5802927. - - SAMTOOLS (1.16.1, [MIT/Expat](https://github.com/samtools/samtools/blob/develop/LICENSE)) - > Danecek P, Bonfield JK, Liddle J, Marshall J, Ohan V, Pollard MO, Whitwham A, Keane T, McCarthy SA, M Davies RM, Li H. 2021. Twelve years of SAMtools and BCFtools, GigaScience, Volume 10, Issue 2, February 2021, giab008, +- JUICEBOX.JS, [MIT](https://github.com/igvteam/juicebox.js/blob/master/LICENSE) + + > Robinson JT, Turner D, Durand NC, Thorvaldsdóttir H, Mesirov JP, Aiden EL. 2018. Juicebox.js Provides a Cloud-Based Visualization System for Hi-C Data. Cell Syst. 2018 Feb 28;6(2):256-258.e1. doi: . Epub 2018 Feb 7. PMID: 29428417; PMCID: PMC6047755. + +- FASTP, [MIT](https://github.com/OpenGene/fastp/blob/master/LICENSE) + + > Chen S, Zhou Y, Chen Y, Gu J. 2018. fastp: an ultra-fast all-in-one FASTQ preprocessor, Bioinformatics, Volume 34, Issue 17, 01 September 2018, Pages i884–i890, + +- FASTQC, [GPL v3](https://github.com/s-andrews/FastQC/blob/master/LICENSE.txt) + + > + +- run-assembly-visualizer.sh, [MIT](https://github.com/aidenlab/3d-dna/blob/master/LICENSE) + + > Dudchenko O, Batra SS, Omer AD, Nyquist SK, Hoeger M, Durand NC, Shamim MS, Machol I, Lander, Aiden AP, Aiden EL 2017. De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds.Science356, 92-95(2017). doi: . Available at: + +- HIC_QC, [AGPL v3](https://github.com/phasegenomics/hic_qc/blob/master/LICENSE) + + > + +- JUICEBOX_SCRIPTS, [AGPL v3](https://github.com/phasegenomics/juicebox_scripts/blob/master/LICENSE) + + > + +- BWA, [GPL v3](https://github.com/lh3/bwa/blob/master/COPYING) + + > Li H. 2013. Aligning sequence reads, clone sequences and assembly contigs with BWA-MEM. + +- MATLOCK, [AGPL v3](https://github.com/phasegenomics/matlock/blob/master/LICENSE) + + > ; + +- SAMBLASTER, [MIT](https://github.com/GregoryFaust/samblaster/blob/master/LICENSE.txt) + + > Faust GG, Hall IM. 2014. SAMBLASTER: fast duplicate marking and structural variant read extraction, Bioinformatics, Volume 30, Issue 17, September 2014, Pages 2503–2505, + +- CIRCOS, [GPL v3](https://www.gnu.org/licenses/gpl-3.0.txt) + + > Krzywinski M, Schein J, Birol I, Connors J, Gascoyne R. Horsman D, ... Marra MA. 2009. Circos: an information aesthetic for comparative genomics. Genome research, 19(9), 1639-1645. + +- MUMMER, [Artistic 2.0](https://github.com/mummer4/mummer/blob/master/LICENSE.md) + > Marçais G, Delcher AL, Phillippy AM, Coston R, Salzberg SL, Zimin A. 2018. MUMmer4: A fast and versatile genome alignment system. PLoS Comput Biol. 2018 Jan 26;14(1):e1005944. doi: . PMID: 29373581; PMCID: PMC5802927. diff --git a/README.md b/README.md index 11ba59ff..8564d306 100644 --- a/README.md +++ b/README.md @@ -35,7 +35,6 @@ flowchart LR Skip --> REPORT VALIDATE_FORMAT --> GFF_STATS[GENOMETOOLS GT STAT] - VALIDATE_FORMAT --> GFF_STATS_II[BIOCODE GFF3 STATS] Run --> ASS_STATS[ASSEMBLATHON STATS] Run --> BUSCO @@ -48,7 +47,6 @@ flowchart LR ASS_STATS --> REPORT GFF_STATS --> REPORT - GFF_STATS_II --> REPORT BUSCO --> REPORT TIDK --> REPORT LAI --> REPORT @@ -60,7 +58,7 @@ flowchart LR - [FASTA VALIDATION](https://github.com/GallVp/fasta_validator) - [GFF3 VALIDATION](https://github.com/genometools/genometools) - [ASSEMBLATHON STATS](https://github.com/PlantandFoodResearch/assemblathon2-analysis/blob/a93cba25d847434f7eadc04e63b58c567c46a56d/assemblathon_stats.pl): Assembly statistics -- [GENOMETOOLS GT STAT](https://github.com/genometools/genometools)/[BIOCODE GFF3 STATS](https://github.com/jorvis/biocode): Annotation statistics +- [GENOMETOOLS GT STAT](https://github.com/genometools/genometools): Annotation statistics - [NCBI FCS ADAPTOR](https://github.com/ncbi/fcs): Adaptor contamination pass/fail - [NCBI FCS GX](https://github.com/ncbi/fcs): Foreign organism contamination pass/fail - [BUSCO](https://gitlab.com/ezlab/busco/-/tree/master): Gene-space completeness estimation diff --git a/bin/assembly_qc_report_943e0fb.py b/bin/assembly_qc_report_943e0fb.py index 698026c9..f305101e 100755 --- a/bin/assembly_qc_report_943e0fb.py +++ b/bin/assembly_qc_report_943e0fb.py @@ -1,6 +1,15 @@ -#!/usr/bin/env python +#!/usr/bin/env python3.9 import json +import yaml + +from report_modules.report_printer import ReportPrinter + +from report_modules.parsers.params_parser import parse_params_json +from report_modules.parsers.tools_parser import parse_tools_yaml + +from report_modules.parsers.gff3_validate_parser import parse_gff3_validate_folder +from report_modules.parsers.fasta_validate_parser import parse_fasta_validate_folder from report_modules.parsers.ncbi_fcs_adaptor_parser import parse_ncbi_fcs_adaptor_folder from report_modules.parsers.ncbi_fcs_gx_parser import parse_ncbi_fcs_gx_folder @@ -10,28 +19,25 @@ from report_modules.parsers.genometools_gt_stat_parser import ( parse_genometools_gt_stat_folder, ) -from report_modules.parsers.biocode_gff3_stats_parser import ( - parse_biocode_gff3_stats_folder, -) from report_modules.parsers.busco_parser import parse_busco_folder from report_modules.parsers.tidk_parser import parse_tidk_folder from report_modules.parsers.lai_parser import parse_lai_folder from report_modules.parsers.kraken2_parser import parse_kraken2_folder from report_modules.parsers.hic_parser import parse_hic_folder from report_modules.parsers.circos_parser import parse_circos_folder -from report_modules.report_printer import ReportPrinter -from report_modules.parsers.params_parser import parse_params_json if __name__ == "__main__": params_dict, params_table = parse_params_json() + tools_dict, tools_table = parse_tools_yaml() data_from_tools = {} + data_from_tools = {**data_from_tools, **parse_gff3_validate_folder()} + data_from_tools = {**data_from_tools, **parse_fasta_validate_folder()} data_from_tools = {**data_from_tools, **parse_ncbi_fcs_adaptor_folder()} data_from_tools = {**data_from_tools, **parse_ncbi_fcs_gx_folder()} data_from_tools = {**data_from_tools, **parse_assemblathon_stats_folder()} data_from_tools = {**data_from_tools, **parse_genometools_gt_stat_folder()} - data_from_tools = {**data_from_tools, **parse_biocode_gff3_stats_folder()} data_from_tools = {**data_from_tools, **parse_busco_folder()} data_from_tools = {**data_from_tools, **parse_tidk_folder()} data_from_tools = {**data_from_tools, **parse_lai_folder()} @@ -39,25 +45,19 @@ data_from_tools = {**data_from_tools, **parse_hic_folder()} data_from_tools = {**data_from_tools, **parse_circos_folder()} + with open("software_versions.yml", "r") as f: + versions_from_ch_versions = yaml.safe_load(f) + data_from_tools = { - **data_from_tools, - "VERSIONS": { - "SELF": "v1.4", - "NCBI_FCS_ADAPTOR": "0.4", - "NCBI_FCS_GX": "0.4", - "ASSEMBLATHON_STATS": "github/PlantandFoodResearch/assemblathon2-analysis/a93cba2", - "GENOMETOOLS_GT_STAT": "1.6.2", - "BIOCODE_GFF3_STATS": "0.10.0", - "BUSCO": "5.2.2", - "TIDK": "0.2.31", - "LAI": "beta3.2", - "KRAKEN2": "2.1.2", - "HIC": "2.4.3", - "CIRCOS": "0.23-1", - "MUMMER": "4.0.0", - }, "PARAMS_DICT": params_dict, "PARAMS_TABLE": params_table, + "TOOLS_DICT": tools_dict, + "TOOLS_TABLE": tools_table, + "VERSIONS": { + **versions_from_ch_versions, + "JUICEBOX_JS": "2.4.3", + }, + **data_from_tools, } report_printer = ReportPrinter() diff --git a/bin/check_gff3_fasta_corresp_3031aca.sh b/bin/check_gff3_fasta_corresp_3031aca.sh deleted file mode 100755 index f302be2b..00000000 --- a/bin/check_gff3_fasta_corresp_3031aca.sh +++ /dev/null @@ -1,54 +0,0 @@ -#!/usr/bin/env bash - -## Inputs -fasta_file="$1" -gff3_file="$2" - -# Requires -# samtools faidx - -## STEP 1 -# Check that gff3 has no identifers that are not in fasta (fasta can -# have ids that are not in gff3 since not all assembly units have gff3 records - -# Extract identifiers from the GFF3 file -gff3_identifiers=$(grep -v '^#' "$gff3_file" | awk '{print $1}' | sort -u) - -# Extract identifiers from the FASTA file -fasta_identifiers=$(grep '^>' "$fasta_file" | awk '{print substr($1, 2)}' | sort -u) - -# Compare identifiers and find any that are present in the GFF3 but not in the FASTA -missing_identifiers=$(comm -23 <(echo "$gff3_identifiers") <(echo "$fasta_identifiers")) - -# Check if any missing identifiers were found -if [[ -n "$missing_identifiers" ]]; then - echo "Failed to validate gff3 file for: $tag_label" - echo "Fasta file: $fasta_file" - echo "Gff3 file: $gff3_file" - echo "GFF3 file contains identifiers not present in FASTA:" - echo "$missing_identifiers" - exit 1 -fi - -## STEP 2 -# check that there are no coordiantes in gff3 for any seqid that are -# greater than the seq length of the paretn fasta entry - -# Compute sequence lengths using samtools faidx -samtools faidx "$fasta_file" | cut -f 1,2 > sequence_lengths.txt - -# Check GFF3 file for coordinates exceeding sequence lengths -while IFS=$'\t' read -r seqname source feature start end score strand frame attributes && \ - read -r seq seq_length <&3; do - if [[ $start -gt $seq_length || $end -gt $seq_length ]]; then - echo "Failed to validate gff3 file for: $tag_label" - echo "Fasta file: $fasta_file" - echo "Gff3 file: $gff3_file" - echo "Coordinates exceed sequence length in GFF3 file:" - echo "Sequence: $seqname" - echo "Sequence length: $seq_length" - echo "Start: $start" - echo "End: $end" - exit 1 - fi -done < "$gff3_file" 3< "sequence_lengths.txt" diff --git a/bin/report_modules/parsers/biocode_gff3_stats_parser.py b/bin/report_modules/parsers/biocode_gff3_stats_parser.py deleted file mode 100644 index 01925375..00000000 --- a/bin/report_modules/parsers/biocode_gff3_stats_parser.py +++ /dev/null @@ -1,214 +0,0 @@ -import os -import re -import base64 -from pathlib import Path -from tabulate import tabulate -import pandas as pd -import numpy as np -import matplotlib.pyplot as plt -import math - -from report_modules.parsers.parsing_commons import sort_list_of_results - - -def parse_gff3_statistics(file_lines): - general_stats = [] - cds_stats = [] - - read_lines = 0 - for line in file_lines: - read_lines += 1 - - if line.startswith("Skipping feature"): - continue - - if line == "\n" or len(line) < 1: - continue - - if line.startswith("# CDS fragment composition profile"): - break - - line_components = line.split("\t") - - # Return None as the parsing assumptions are not valid anymore. - # The file is not parsable. - if len(line_components) != 2: - return None - - key = line_components[0] - value = line_components[1] - - if key == "Assembly length": - continue - - general_stats.append((key, int(round(float(value))))) - - for line in file_lines[read_lines - 1 :]: - if line.startswith("# CDS fragment composition profile"): - continue - - if line == "\n" or len(line) < 1: - continue - - key, value, percentage = line.split("\t") - cds_stats.append((int(key.split(" ")[2]), int(value), float(percentage))) - - general_stats_table = pd.DataFrame(general_stats, columns=["Metric", "Value"]) - cds_stats_table = pd.DataFrame( - cds_stats, columns=["CDS Count", "mRNA Count", "Percentage"] - ) - - return general_stats_table, cds_stats_table - - -def create_bar_graph(cds_stats_table, file_name): - _, ax = plt.subplots() - ax.bar(cds_stats_table["CDS Count"], cds_stats_table["mRNA Count"]) - - ax.set_xlabel("CDS Count") - ax.set_ylabel("mRNA Count") - ax.set_title("CDS fragment composition profile") - - num_ticks = 16.0 - min_x = float(min(cds_stats_table["CDS Count"])) - max_x = float(max(cds_stats_table["CDS Count"])) - setp_x = math.ceil((max_x - min_x) / num_ticks) - plt.xticks(np.arange(int(min_x), int(max_x) + setp_x, setp_x)) - - plt.gca().spines["top"].set_visible(False) - plt.gca().spines["right"].set_visible(False) - - offset = 0.01 * max(cds_stats_table["mRNA Count"]) - - if len(cds_stats_table["CDS Count"]) <= 24: - plt.yticks([]) - plt.ylim(0, max(cds_stats_table["mRNA Count"]) * 1.2) - - for i, value in enumerate(cds_stats_table["mRNA Count"]): - plt.text( - cds_stats_table["CDS Count"].iloc[i], - value + offset, - str(value), - ha="center", - va="bottom", - rotation="vertical", - ) - - plt.gca().spines["left"].set_visible(False) - else: - num_ticks = 10.0 - min_y = float(min(cds_stats_table["mRNA Count"])) - max_y = float(max(cds_stats_table["mRNA Count"])) - setp_y = math.ceil((max_y - min_y) / num_ticks) - plt.yticks(np.arange(int(min_y), int(max_y) + setp_y, setp_y)) - - max_y = cds_stats_table["mRNA Count"].max() - max_y_i = cds_stats_table["mRNA Count"].idxmax() - x_for_max_of_y = cds_stats_table["CDS Count"].iloc[max_y_i] - - plt.text( - x_for_max_of_y, - max_y + offset, - f"Max: {str(max_y)}", - ha="left", - va="baseline", - rotation="horizontal", - ) - - plt.savefig(file_name, dpi=600) - - -def read_file_lines(file_path): - with open(file_path, "r") as f: - file_lines = f.readlines() - - return file_lines - - -def parse_biocode_gff3_stats_folder(folder_name="biocode_gff3_stats"): - dir = os.getcwdb().decode() - reports_folder_path = Path(f"{dir}/{folder_name}") - - if not os.path.exists(reports_folder_path): - return {} - - list_of_report_files = reports_folder_path.glob("*.csv") - - data = {"BIOCODE_GFF3_STATS": []} - - for report_path in list_of_report_files: - file_lines = read_file_lines(report_path) - - file_tag = re.findall( - r"([\w]+)_stats.csv", - os.path.basename(str(report_path)), - )[0] - - parsed_stats = parse_gff3_statistics(file_lines) - - if parsed_stats == None: - data["BIOCODE_GFF3_STATS"].append( - { - "hap": file_tag, - "general_stats_table": {}, - "cds_stats_table": {}, - "general_stats_table_html": '
'
-                    + "\n".join(
-                        ["Failed to parse the BIOCODE GFF3 STATS output:\n\n"]
-                        + file_lines
-                    )
-                    + "
", - "cds_plot": "", - } - ) - continue - - general_stats_table = parsed_stats[0] - cds_stats_table = parsed_stats[1] - - plot_path = f"./{folder_name}/{os.path.basename(report_path)}.png" - create_bar_graph(cds_stats_table, plot_path) - - general_stats_metric = general_stats_table.iloc[:, 0].values.tolist() - general_stats_values = general_stats_table.iloc[:, 1].values.tolist() - - cds_stats_metric = cds_stats_table.iloc[:, 0].values.tolist() - cds_stats_values = cds_stats_table.iloc[:, 1].values.tolist() - cds_stats_percentages = cds_stats_table.iloc[:, 2].values.tolist() - - general_stats_dict = { - f"{x}": f"{y}" for (x, y) in zip(general_stats_metric, general_stats_values) - } - cds_stats_dict = { - f"{x}": [f"{y}", f"{z}"] - for (x, y, z) in zip( - cds_stats_metric, cds_stats_values, cds_stats_percentages - ) - } - - with open(plot_path, "rb") as f: - binary_fc = f.read() - - base64_utf8_str = base64.b64encode(binary_fc).decode("utf-8") - ext = str(plot_path).split(".")[-1] - plot_url = f"data:image/{ext}+xml;base64,{base64_utf8_str}" - - data["BIOCODE_GFF3_STATS"].append( - { - "hap": file_tag, - "general_stats_table": general_stats_dict, - "cds_stats_table": cds_stats_dict, - "general_stats_table_html": tabulate( - general_stats_table, - headers=["Metric", "Value"], - tablefmt="html", - numalign="left", - showindex=False, - ), - "cds_plot": plot_url, - } - ) - - return { - "BIOCODE_GFF3_STATS": sort_list_of_results(data["BIOCODE_GFF3_STATS"], "hap") - } diff --git a/bin/report_modules/parsers/fasta_validate_parser.py b/bin/report_modules/parsers/fasta_validate_parser.py new file mode 100644 index 00000000..786978ec --- /dev/null +++ b/bin/report_modules/parsers/fasta_validate_parser.py @@ -0,0 +1,35 @@ +import os +from pathlib import Path +import re + +from report_modules.parsers.parsing_commons import sort_list_of_results + + +def parse_fasta_validate_folder(folder_name="fastavalidator_logs"): + dir = os.getcwdb().decode() + logs_folder_path = Path(f"{dir}/{folder_name}") + + if not os.path.exists(logs_folder_path): + return {} + + list_of_log_files = logs_folder_path.glob("*.log") + + data = {"FASTA_VALIDATE": []} + + for log_path in list_of_log_files: + with open(log_path, "r") as f: + log_lines = [f"

{l}

" for l in f.readlines()] + + file_tokens = re.findall( + r"([\w]+).error.log", + os.path.basename(str(log_path)), + )[0] + + data["FASTA_VALIDATE"].append( + { + "hap": file_tokens, + "validation_log": "".join(log_lines), + } + ) + + return {"FASTA_VALIDATE": sort_list_of_results(data["FASTA_VALIDATE"], "hap")} diff --git a/bin/report_modules/parsers/genometools_gt_stat_parser.py b/bin/report_modules/parsers/genometools_gt_stat_parser.py index 4fc80402..a4e4e6f4 100644 --- a/bin/report_modules/parsers/genometools_gt_stat_parser.py +++ b/bin/report_modules/parsers/genometools_gt_stat_parser.py @@ -3,6 +3,9 @@ import pandas as pd from tabulate import tabulate import re +import matplotlib.pyplot as plt +import numpy as np +import base64 from report_modules.parsers.parsing_commons import sort_list_of_results @@ -14,37 +17,328 @@ def parse_genometools_gt_stat_folder(folder_name="genometools_gt_stat"): if not os.path.exists(reports_folder_path): return {} - list_of_report_files = reports_folder_path.glob("*.csv") + list_of_report_files = reports_folder_path.glob("*.yml") data = {"GENOMETOOLS_GT_STAT": []} for report_path in list_of_report_files: - report_table = pd.read_csv(report_path) - stat_names = report_table.iloc[:, 0].values.tolist() - stat_values = report_table.iloc[:, 1].values.tolist() + NUM_GROUPS = -1 + ( + report_table_dict, + gene_length_distribution, + gene_score_distribution, + exon_length_distribution, + exon_number_distribution, + intron_length_distribution, + cds_length_distribution, + ) = extract_report_data(report_path, NUM_GROUPS) - report_table_dict = {f"{x}": f"{y}" for (x, y) in zip(stat_names, stat_values)} + gene_length_distribution_graph = "" + if gene_length_distribution != []: + gene_length_distribution_graph = create_dist_graph( + gene_length_distribution, + "Length", + "Gene Length Distribution", + f"./{folder_name}/{os.path.basename(report_path)}.gene.length.png", + ) - file_tokens = re.findall( - r"([\w]+)_stats.csv", + gene_score_distribution_graph = "" + if gene_score_distribution != []: + gene_score_distribution_graph = create_dist_graph( + gene_score_distribution, + "Score", + "Gene Score Distribution", + f"./{folder_name}/{os.path.basename(report_path)}.gene.score.png", + ) + + exon_length_distribution_graph = "" + if exon_length_distribution != []: + exon_length_distribution_graph = create_dist_graph( + exon_length_distribution, + "Length", + "Exon Length Distribution", + f"./{folder_name}/{os.path.basename(report_path)}.exon.length.png", + ) + + exon_number_distribution_graph = "" + if exon_number_distribution != []: + exon_number_distribution_graph = create_dist_graph( + exon_number_distribution, + "Number", + "Exon Number Distribution", + f"./{folder_name}/{os.path.basename(report_path)}.exon.number.png", + ) + + intron_length_distribution_graph = "" + if intron_length_distribution != []: + intron_length_distribution_graph = create_dist_graph( + intron_length_distribution, + "Length", + "Intron Length Distribution", + f"./{folder_name}/{os.path.basename(report_path)}.intron.length.png", + ) + + cds_length_distribution_graph = "" + if cds_length_distribution != []: + cds_length_distribution_graph = create_dist_graph( + intron_length_distribution, + "Length", + "CDS Length Distribution", + f"./{folder_name}/{os.path.basename(report_path)}.cds.length.png", + ) + + file_tag = re.findall( + r"([\w]+).gt.stat.yml", os.path.basename(str(report_path)), )[0] data["GENOMETOOLS_GT_STAT"].append( { - "hap": file_tokens, + "hap": file_tag, "report_table": report_table_dict, "report_table_html": tabulate( - report_table, + pd.DataFrame( + report_table_dict.items(), columns=["Metric", "Value"] + ), headers=["Stat", "Value"], tablefmt="html", numalign="left", showindex=False, ), + "gene_length_plot": gene_length_distribution_graph, + "gene_score_plot": gene_score_distribution_graph, + "exon_length_plot": exon_length_distribution_graph, + "exon_number_plot": exon_number_distribution_graph, + "intron_length_plot": intron_length_distribution_graph, + "cds_length_plot": cds_length_distribution_graph, } ) return { "GENOMETOOLS_GT_STAT": sort_list_of_results(data["GENOMETOOLS_GT_STAT"], "hap") } + + +def extract_report_data(report_path, num_groups): + yaml_data = {} + parent_key = "" + with open(report_path, "r") as stream: + for line in stream: + key, value = line.strip().split(":", 1) + + if value == "": + parent_key = key + yaml_data[parent_key] = {} + continue + + if parent_key == "": + yaml_data[key] = value.strip() + continue + + yaml_data[parent_key][key] = value.strip() + + report_table_dict = { + key: value for key, value in yaml_data.items() if not isinstance(value, dict) + } + gene_length_distribution = create_frequency_groups( + [ + (int(key), int(value.split("(")[0].strip())) + for key, value in yaml_data["gene length distribution"].items() + ], + num_groups, + ) + gene_score_distribution = create_frequency_groups( + [ + (int(key), int(value.split("(")[0].strip())) + for key, value in yaml_data["gene score distribution"].items() + ], + num_groups, + ) + exon_length_distribution = create_frequency_groups( + [ + (int(key), int(value.split("(")[0].strip())) + for key, value in yaml_data["exon length distribution"].items() + ], + num_groups, + ) + exon_number_distribution = create_frequency_groups( + [ + (int(key), int(value.split("(")[0].strip())) + for key, value in yaml_data["exon number distribution"].items() + ], + num_groups, + ) + intron_length_distribution = create_frequency_groups( + [ + (int(key), int(value.split("(")[0].strip())) + for key, value in yaml_data["intron length distribution"].items() + ], + num_groups, + ) + cds_length_distribution = create_frequency_groups( + [ + (int(key), int(value.split("(")[0].strip())) + for key, value in yaml_data["CDS length distribution"].items() + ], + num_groups, + ) + + return ( + report_table_dict, + gene_length_distribution, + gene_score_distribution, + exon_length_distribution, + exon_number_distribution, + intron_length_distribution, + cds_length_distribution, + ) + + +def create_frequency_groups(data, num_groups): + + if num_groups == -1: + sorted_data = sorted(data, key=lambda x: x[0]) + return [ + { + "start": x, + "stop": x, + "freq": freq, + } + for x, freq in sorted_data + ] + + assert ( + num_groups >= 1 + ), f"num_groups should be larger than or equal to 1. It is {num_groups}" + + if data == []: + return [] + + sorted_data = sorted(data, key=lambda x: x[0]) + + ordinal = [x for x, _ in sorted_data] + + ordinal_max = max(ordinal) + ordinal_range = ordinal_max - min(ordinal) + ordinal_step = ordinal_range // num_groups + + groups = [] + current_group = { + "start": sorted_data[0][0], + "stop": [x for x in ordinal if x <= (sorted_data[0][0] + ordinal_step)][-1], + "freq": 0, + } + + for num, freq in sorted_data: + if num <= current_group["stop"]: + current_group["freq"] += freq + continue + + groups.append(current_group.copy()) + + current_group["start"] = num + current_group["stop"] = [x for x in ordinal if x <= (num + ordinal_step)][-1] + current_group["freq"] = freq + + groups.append(current_group) + + return groups + + +def test_create_frequency_groups_multiple(): + data = [(15, 4), (5, 1), (70, 10)] + num_groups = 2 + + expect = [ + {"start": 5, "stop": 15, "freq": 5}, + {"start": 70, "stop": 70, "freq": 10}, + ] + + assert expect == create_frequency_groups(data, num_groups) + + +def test_create_frequency_groups_single(): + data = [(15, 4)] + num_groups = 2 + + expect = [{"start": 15, "stop": 15, "freq": 4}] + + assert expect == create_frequency_groups(data, num_groups) + + +def test_create_frequency_groups_repeat(): + data = [(15, 4), (15, 8)] + num_groups = 2 + + expect = [{"start": 15, "stop": 15, "freq": 12}] + + assert expect == create_frequency_groups(data, num_groups) + + +# test_create_frequency_groups_multiple() +# test_create_frequency_groups_single() +# test_create_frequency_groups_repeat() + + +def create_dist_graph(groups_dict, x_label, title, file_name): + + x_list = [i["stop"] for i in groups_dict] + y_list = [i["freq"] for i in groups_dict] + sum_y = float(sum(y_list)) + cum_sum_y = np.cumsum(y_list) + y_list = [float(y) / sum_y * 100.0 for y in cum_sum_y] + + _, ax = plt.subplots() + ax.plot(x_list, y_list) + + ax.set_xlabel(x_label) + ax.set_ylabel("Cumulative percentage (%)") + ax.set_title(title) + + plt.gca().spines["top"].set_visible(False) + plt.gca().spines["right"].set_visible(False) + + min_x, min_y = (min(x_list), min(y_list)) + x_anno_step = int(float(max(x_list)) * 0.1) + ax.annotate( + f"(<={min_x}, {round(min_y, 2)}%)", + xy=(min_x, min_y), + xytext=(min_x + x_anno_step, min_y + 10), + arrowprops=dict(color="red", arrowstyle="->, head_width=.15"), + ) + + near_50 = min([y for y in y_list if y >= 50.0]) + min_x, min_y = (x_list[y_list.index(near_50)], near_50) + ax.annotate( + f"(<={min_x}, {round(min_y, 2)}%)", + xy=(min_x, min_y), + xytext=(min_x + x_anno_step, min_y), + arrowprops=dict(color="red", arrowstyle="->, head_width=.15"), + ) + + near_90 = min([y for y in y_list if y >= 90.0]) + min_x, min_y = (x_list[y_list.index(near_90)], near_90) + ax.annotate( + f"(<={min_x}, {round(min_y, 2)}%)", + xy=(min_x, min_y), + xytext=(min_x + x_anno_step, min_y - 10), + arrowprops=dict(color="red", arrowstyle="->, head_width=.15"), + ) + + near_3_sigma = min([y for y in y_list if y >= 99.7]) + min_x, min_y = (x_list[y_list.index(near_3_sigma)], near_3_sigma) + ax.annotate( + f"(<={min_x}, {round(min_y, 2)}%)", + xy=(min_x, min_y), + xytext=(min_x + x_anno_step, min_y - 10), + arrowprops=dict(color="red", arrowstyle="->, head_width=.15"), + ) + + plt.savefig(file_name, dpi=600) + + with open(file_name, "rb") as f: + binary_fc = f.read() + + base64_utf8_str = base64.b64encode(binary_fc).decode("utf-8") + return f"data:image/png+xml;base64,{base64_utf8_str}" diff --git a/bin/report_modules/parsers/gff3_validate_parser.py b/bin/report_modules/parsers/gff3_validate_parser.py new file mode 100644 index 00000000..e9a7d1e7 --- /dev/null +++ b/bin/report_modules/parsers/gff3_validate_parser.py @@ -0,0 +1,35 @@ +import os +from pathlib import Path +import re + +from report_modules.parsers.parsing_commons import sort_list_of_results + + +def parse_gff3_validate_folder(folder_name="gff3_validate_logs"): + dir = os.getcwdb().decode() + logs_folder_path = Path(f"{dir}/{folder_name}") + + if not os.path.exists(logs_folder_path): + return {} + + list_of_log_files = logs_folder_path.glob("*.log") + + data = {"GFF3_VALIDATE": []} + + for log_path in list_of_log_files: + with open(log_path, "r") as f: + log_lines = [f"

{l}

" for l in f.readlines()] + + file_tokens = re.findall( + r"([\w]+).error.log", + os.path.basename(str(log_path)), + )[0] + + data["GFF3_VALIDATE"].append( + { + "hap": file_tokens, + "validation_log": "".join(log_lines), + } + ) + + return {"GFF3_VALIDATE": sort_list_of_results(data["GFF3_VALIDATE"], "hap")} diff --git a/bin/report_modules/parsers/tools_parser.py b/bin/report_modules/parsers/tools_parser.py new file mode 100644 index 00000000..54746094 --- /dev/null +++ b/bin/report_modules/parsers/tools_parser.py @@ -0,0 +1,33 @@ +import yaml +import json + +from pygments import highlight +from pygments.lexers import JsonLexer +from pygments.formatters import HtmlFormatter + + +def parse_tools_yaml(): + with open("software_versions.yml", "r") as f: + tools_dict = yaml.safe_load(f) + formatted_tools_json = highlight_json( + json.dumps(format_tools_dict(tools_dict), indent=4) + ) + + return tools_dict, formatted_tools_json + + +def highlight_json(json_string): + lexer = JsonLexer() + formatter = HtmlFormatter() + + return highlight(json_string, lexer, formatter) + + +def format_tools_dict(input_dict): + output_list = [] + for _, top_level_value in input_dict.items(): + for key, value in top_level_value.items(): + if (key, value) not in output_list: + output_list.append((key, value)) + + return dict(sorted(output_list, key=lambda x: x[0])) diff --git a/bin/report_modules/templates/assemblathon_stats/assemblathon_stats.html b/bin/report_modules/templates/assemblathon_stats/assemblathon_stats.html index 108e8f13..d8e5b82d 100644 --- a/bin/report_modules/templates/assemblathon_stats/assemblathon_stats.html +++ b/bin/report_modules/templates/assemblathon_stats/assemblathon_stats.html @@ -7,7 +7,9 @@ >https://github.com/KorfLab/Assemblathon

-

Version: {{ all_stats_dicts['VERSIONS']['ASSEMBLATHON_STATS'] }}

+

+ Version: {{ all_stats_dicts['VERSIONS']['ASSEMBLATHON_STATS']['assemblathon_stats'] }} +

Warning:

Contig-related stats are based on the assumption that the n_limit ({{ diff --git a/bin/report_modules/templates/base.html b/bin/report_modules/templates/base.html index f2b6e872..e0b8eef0 100644 --- a/bin/report_modules/templates/base.html +++ b/bin/report_modules/templates/base.html @@ -3,16 +3,18 @@

-
AssemblyQC {{ all_stats_dicts['VERSIONS']['SELF'] }}
+
AssemblyQC {{ all_stats_dicts['VERSIONS']['Workflow']['AssemblyQC'] }}
- {% if 'NCBI_FCS_ADAPTOR' in all_stats_dicts %}{% endif %} {% if 'NCBI_FCS_GX' in all_stats_dicts %} + {% if 'GFF3_VALIDATE' in all_stats_dicts %} + + {% endif %} {% if 'FASTA_VALIDATE' in all_stats_dicts %} + + {% endif %} {% if 'NCBI_FCS_ADAPTOR' in all_stats_dicts %} + + {% endif %} {% if 'NCBI_FCS_GX' in all_stats_dicts %}{% endif %} {% if 'BIOCODE_GFF3_STATS' in all_stats_dicts %}{% endif %} {% if 'BUSCO' in all_stats_dicts %}
- {% include 'params/params.html' %} {% if 'NCBI_FCS_ADAPTOR' in all_stats_dicts %}{% include - 'ncbi_fcs_adaptor/ncbi_fcs_adaptor.html' %}{% endif %} {% if 'NCBI_FCS_GX' in all_stats_dicts %}{% include - 'ncbi_fcs_gx/ncbi_fcs_gx.html' %}{% endif %} {% if 'ASSEMBLATHON_STATS' in all_stats_dicts %}{% include + {% include 'params/params.html' %} {% include 'tools/tools.html' %} {% if 'GFF3_VALIDATE' in all_stats_dicts + %}{% include 'gff3_validate/gff3_validate.html' %}{% endif %} {% if 'FASTA_VALIDATE' in all_stats_dicts %}{% + include 'fasta_validate/fasta_validate.html' %}{% endif %} {% if 'NCBI_FCS_ADAPTOR' in all_stats_dicts %}{% + include 'ncbi_fcs_adaptor/ncbi_fcs_adaptor.html' %}{% endif %} {% if 'NCBI_FCS_GX' in all_stats_dicts %}{% + include 'ncbi_fcs_gx/ncbi_fcs_gx.html' %}{% endif %} {% if 'ASSEMBLATHON_STATS' in all_stats_dicts %}{% include 'assemblathon_stats/assemblathon_stats.html' %}{% endif %} {% if 'GENOMETOOLS_GT_STAT' in all_stats_dicts %}{% - include 'genometools_gt_stat/genometools_gt_stat.html' %}{% endif %} {% if 'BIOCODE_GFF3_STATS' in - all_stats_dicts %}{% include 'biocode_gff3_stats/biocode_gff3_stats.html' %}{% endif %} {% if 'BUSCO' in - all_stats_dicts %}{% include 'busco/busco.html' %}{% endif %} {% if 'TIDK' in all_stats_dicts %}{% include - 'tidk/tidk.html' %}{% endif %} {% if 'LAI' in all_stats_dicts %}{% include 'lai/lai.html' %}{% endif %} {% if - 'KRAKEN2' in all_stats_dicts %}{% include 'kraken2/kraken2.html' %}{% endif %} {% if 'HIC' in all_stats_dicts - %}{% include 'hic/hic.html' %}{% endif %} {% if 'CIRCOS' in all_stats_dicts %}{% include 'circos/circos.html' - %}{% endif %} + include 'genometools_gt_stat/genometools_gt_stat.html' %}{% endif %} {% if 'BUSCO' in all_stats_dicts %}{% + include 'busco/busco.html' %}{% endif %} {% if 'TIDK' in all_stats_dicts %}{% include 'tidk/tidk.html' %}{% + endif %} {% if 'LAI' in all_stats_dicts %}{% include 'lai/lai.html' %}{% endif %} {% if 'KRAKEN2' in + all_stats_dicts %}{% include 'kraken2/kraken2.html' %}{% endif %} {% if 'HIC' in all_stats_dicts %}{% include + 'hic/hic.html' %}{% endif %} {% if 'CIRCOS' in all_stats_dicts %}{% include 'circos/circos.html' %}{% endif %} {% include 'js.html' %} diff --git a/bin/report_modules/templates/biocode_gff3_stats/biocode_gff3_stats.html b/bin/report_modules/templates/biocode_gff3_stats/biocode_gff3_stats.html deleted file mode 100644 index 711b74ab..00000000 --- a/bin/report_modules/templates/biocode_gff3_stats/biocode_gff3_stats.html +++ /dev/null @@ -1,13 +0,0 @@ - diff --git a/bin/report_modules/templates/biocode_gff3_stats/dropdown.html b/bin/report_modules/templates/biocode_gff3_stats/dropdown.html deleted file mode 100644 index 33f97326..00000000 --- a/bin/report_modules/templates/biocode_gff3_stats/dropdown.html +++ /dev/null @@ -1,10 +0,0 @@ -
-
- -
-
diff --git a/bin/report_modules/templates/biocode_gff3_stats/report_contents.html b/bin/report_modules/templates/biocode_gff3_stats/report_contents.html deleted file mode 100644 index d5acfe56..00000000 --- a/bin/report_modules/templates/biocode_gff3_stats/report_contents.html +++ /dev/null @@ -1,20 +0,0 @@ -{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["BIOCODE_GFF3_STATS"]|length) %} {% set -active_text = 'display: block' if vars.is_first else 'display: none' %} -
-
-
-
{{ all_stats_dicts['BIOCODE_GFF3_STATS'][item]['hap'] }}
-
-
-
-
{{ all_stats_dicts['BIOCODE_GFF3_STATS'][item]['general_stats_table_html'] }}
-
-
- -
-
-{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/bin/report_modules/templates/busco/busco.html b/bin/report_modules/templates/busco/busco.html index c8a180a1..06d560e5 100644 --- a/bin/report_modules/templates/busco/busco.html +++ b/bin/report_modules/templates/busco/busco.html @@ -9,9 +9,9 @@ Manni M., Berkeley M.R., Seppey M., Simao F.A., Zdobnov E.M. 2021. BUSCO update: novel and streamlined workflows along with broader and deeper phylogenetic coverage for scoring of eukaryotic, prokaryotic, and viral genomes. arXiv:2106.11799 [q-bio] [Internet]. Available from: - http://arxiv.org/abs/2106.11799 + arxiv.org/abs/2106.11799

-

Version: {{ all_stats_dicts['VERSIONS']['BUSCO'] }}

+

Version: {{ all_stats_dicts['VERSIONS']['RUN_BUSCO']['busco'] }}

{% include 'busco/dropdown.html' %} {% include 'busco/summary_contents.html' %} {% include 'busco/report_contents.html' %} diff --git a/bin/report_modules/templates/circos/circos.html b/bin/report_modules/templates/circos/circos.html index 959b9841..2099a251 100644 --- a/bin/report_modules/templates/circos/circos.html +++ b/bin/report_modules/templates/circos/circos.html @@ -8,19 +8,17 @@

Krzywinski, M., Schein, J., Birol, I., Connors, J., Gascoyne, R., Horsman, D., ... & Marra, M. A. (2009). Circos: an information aesthetic for comparative genomics. Genome research, 19(9), 1639-1645. - https://doi.org/10.1101/gr.092759.109 + 10.1101/gr.092759.109

Marçais G, Delcher AL, Phillippy AM, Coston R, Salzberg SL, Zimin A. MUMmer4: A fast and versatile genome alignment system. PLoS Comput Biol. 2018 Jan 26;14(1):e1005944. - https://doi.org/10.1371/journal.pcbi.1005944 + 10.1371/journal.pcbi.1005944

Version: {{ all_stats_dicts['VERSIONS']['CIRCOS'] }} (CIRCOS), {{ all_stats_dicts['VERSIONS']['MUMMER'] - }} (MUMMER)Versions: {{ all_stats_dicts['VERSIONS']['CIRCOS']['circos'] }} (CIRCOS), {{ + all_stats_dicts['VERSIONS']['MUMMER']['nucmer'] }} (MUMMER)

Notes:

diff --git a/bin/report_modules/templates/fasta_validate/dropdown.html b/bin/report_modules/templates/fasta_validate/dropdown.html new file mode 100644 index 00000000..56fb72ef --- /dev/null +++ b/bin/report_modules/templates/fasta_validate/dropdown.html @@ -0,0 +1,10 @@ +
+
+ +
+
diff --git a/bin/report_modules/templates/fasta_validate/fasta_validate.html b/bin/report_modules/templates/fasta_validate/fasta_validate.html new file mode 100644 index 00000000..e0643542 --- /dev/null +++ b/bin/report_modules/templates/fasta_validate/fasta_validate.html @@ -0,0 +1,14 @@ + diff --git a/bin/report_modules/templates/fasta_validate/report_contents.html b/bin/report_modules/templates/fasta_validate/report_contents.html new file mode 100644 index 00000000..7abaee35 --- /dev/null +++ b/bin/report_modules/templates/fasta_validate/report_contents.html @@ -0,0 +1,18 @@ +{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["FASTA_VALIDATE"]|length) %} {% set active_text += 'display: block' if vars.is_first else 'display: none' %} +
+
+
+
{{ all_stats_dicts['FASTA_VALIDATE'][item]['hap'] }}
+
+
+

Validation failed!

+
+
{{ all_stats_dicts['FASTA_VALIDATE'][item]['validation_log'] }}
+
+
+{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/bin/report_modules/templates/genometools_gt_stat/genometools_gt_stat.html b/bin/report_modules/templates/genometools_gt_stat/genometools_gt_stat.html index a181d093..c5a5e7a1 100644 --- a/bin/report_modules/templates/genometools_gt_stat/genometools_gt_stat.html +++ b/bin/report_modules/templates/genometools_gt_stat/genometools_gt_stat.html @@ -9,7 +9,7 @@ structured genome annotations. IEEE/ACM Trans Comput Biol Bioinform. 2013 May-Jun;10(3):645-56. doi: 10.1109/TCBB.2013.68. PMID: 24091398.

-

Version: {{ all_stats_dicts['VERSIONS']['GENOMETOOLS_GT_STAT'] }}

+

Version: {{ all_stats_dicts['VERSIONS']['GT_STAT']['genometools'] }}

{% include 'genometools_gt_stat/dropdown.html' %} {% include 'genometools_gt_stat/report_contents.html' %} diff --git a/bin/report_modules/templates/genometools_gt_stat/report_contents.html b/bin/report_modules/templates/genometools_gt_stat/report_contents.html index 0b7f1119..d2975ede 100644 --- a/bin/report_modules/templates/genometools_gt_stat/report_contents.html +++ b/bin/report_modules/templates/genometools_gt_stat/report_contents.html @@ -13,5 +13,23 @@
{{ all_stats_dicts['GENOMETOOLS_GT_STAT'][item]['report_table_html'] }}
+
+ +
+
+ +
+
+ +
+
+ +
+
+ +
+
+ +
{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/bin/report_modules/templates/gff3_validate/dropdown.html b/bin/report_modules/templates/gff3_validate/dropdown.html new file mode 100644 index 00000000..0bece7a0 --- /dev/null +++ b/bin/report_modules/templates/gff3_validate/dropdown.html @@ -0,0 +1,10 @@ +
+
+ +
+
diff --git a/bin/report_modules/templates/gff3_validate/gff3_validate.html b/bin/report_modules/templates/gff3_validate/gff3_validate.html new file mode 100644 index 00000000..01fa2754 --- /dev/null +++ b/bin/report_modules/templates/gff3_validate/gff3_validate.html @@ -0,0 +1,22 @@ + diff --git a/bin/report_modules/templates/gff3_validate/report_contents.html b/bin/report_modules/templates/gff3_validate/report_contents.html new file mode 100644 index 00000000..d61358a2 --- /dev/null +++ b/bin/report_modules/templates/gff3_validate/report_contents.html @@ -0,0 +1,18 @@ +{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["GFF3_VALIDATE"]|length) %} {% set active_text += 'display: block' if vars.is_first else 'display: none' %} +
+
+
+
{{ all_stats_dicts['GFF3_VALIDATE'][item]['hap'] }}
+
+
+

Validation failed!

+
+
{{ all_stats_dicts['GFF3_VALIDATE'][item]['validation_log'] }}
+
+
+{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/bin/report_modules/templates/hic/hic.html b/bin/report_modules/templates/hic/hic.html index 2937ce52..868dc089 100644 --- a/bin/report_modules/templates/hic/hic.html +++ b/bin/report_modules/templates/hic/hic.html @@ -9,11 +9,10 @@

Robinson JT, Turner D, Durand NC, Thorvaldsdóttir H, Mesirov JP, Aiden EL. Juicebox.js Provides a Cloud-Based Visualization System for Hi-C Data. Cell Syst. 2018 Feb 28;6(2):256-258.e1. - https://doi.org/10.1016/j.cels.2018.01.001. Epub 2018 Feb 7. PMID: 29428417; PMCID: PMC6047755. + 10.1016/j.cels.2018.01.001. Epub + 2018 Feb 7. PMID: 29428417; PMCID: PMC6047755.

-

Version: {{ all_stats_dicts['VERSIONS']['HIC'] }}

+

Version: {{ all_stats_dicts['VERSIONS']['JUICEBOX_JS'] }}

{% include 'hic/dropdown.html' %} {% include 'hic/report_contents.html' %} diff --git a/bin/report_modules/templates/kraken2/kraken2.html b/bin/report_modules/templates/kraken2/kraken2.html index ec12d3e8..1b87e9e2 100644 --- a/bin/report_modules/templates/kraken2/kraken2.html +++ b/bin/report_modules/templates/kraken2/kraken2.html @@ -7,11 +7,9 @@

Reference:

Wood, D.E., Lu, J. & Langmead, B. Improved metagenomic analysis with Kraken 2. Genome Biol 20, 257 (2019). - https://doi.org/10.1186/s13059-019-1891-0 + 10.1186/s13059-019-1891-0

-

Version: {{ all_stats_dicts['VERSIONS']['KRAKEN2'] }}

+

Version: {{ all_stats_dicts['VERSIONS']['RUN_KRAKEN2']['kraken2'] }}

{% include 'kraken2/dropdown.html' %} {% include 'kraken2/report_contents.html' %} diff --git a/bin/report_modules/templates/lai/lai.html b/bin/report_modules/templates/lai/lai.html index ebe389f9..4b0775ae 100644 --- a/bin/report_modules/templates/lai/lai.html +++ b/bin/report_modules/templates/lai/lai.html @@ -12,9 +12,9 @@

Shujun Ou, Jinfeng Chen, Ning Jiang, Assessing genome assembly quality using the LTR Assembly Index (LAI), Nucleic Acids Research, Volume 46, Issue 21, 30 November 2018, Page e126, - https://doi.org/10.1093/nar/gky730 + 10.1093/nar/gky730

-

Version: {{ all_stats_dicts['VERSIONS']['LAI'] }}

+

Version: {{ all_stats_dicts['VERSIONS']['LAI']['lai'] }}

{% if all_stats_dicts['PARAMS_DICT']['lai']['mode'] == '-qq' %}

Warning:

diff --git a/bin/report_modules/templates/ncbi_fcs_adaptor/ncbi_fcs_adaptor.html b/bin/report_modules/templates/ncbi_fcs_adaptor/ncbi_fcs_adaptor.html index 9246ccb3..e62678c9 100644 --- a/bin/report_modules/templates/ncbi_fcs_adaptor/ncbi_fcs_adaptor.html +++ b/bin/report_modules/templates/ncbi_fcs_adaptor/ncbi_fcs_adaptor.html @@ -5,7 +5,7 @@

https://github.com/ncbi/fcs

-

Version: {{ all_stats_dicts['VERSIONS']['NCBI_FCS_ADAPTOR'] }}

+

Version: {{ all_stats_dicts['VERSIONS']['NCBI_FCS_ADAPTOR']['av_screen_x'] }}

{% include 'ncbi_fcs_adaptor/dropdown.html' %} {% include 'ncbi_fcs_adaptor/report_contents.html' %} diff --git a/bin/report_modules/templates/ncbi_fcs_gx/ncbi_fcs_gx.html b/bin/report_modules/templates/ncbi_fcs_gx/ncbi_fcs_gx.html index 097f48c8..5d34fd49 100644 --- a/bin/report_modules/templates/ncbi_fcs_gx/ncbi_fcs_gx.html +++ b/bin/report_modules/templates/ncbi_fcs_gx/ncbi_fcs_gx.html @@ -7,11 +7,10 @@ Mozes, Pooja K Strope, Pape M Sylla, Lukas Wagner, Shelby L Bidwell, Karen Clark, Emily W Davis, Brian Smith-White, Wratko Hlavina, Kim D Pruitt, Valerie A Schneider, Terence D Murphy bioRxiv 2023.06.02.543519; doi: - https://doi.org/10.1101/2023.06.02.543519, GitHub: https://github.com/ncbi/fcs + 10.1101/2023.06.02.543519, GitHub: + https://github.com/ncbi/fcs

-

Version: {{ all_stats_dicts['VERSIONS']['NCBI_FCS_GX'] }}

+

Version: {{ all_stats_dicts['VERSIONS']['SCREEN_SAMPLES']['fcs_gx'] }}

DB Version: {{ all_stats_dicts['NCBI_FCS_GX'][0]['report_meta_data'][1]['db']['build-date'] }}

diff --git a/bin/report_modules/templates/params/params.html b/bin/report_modules/templates/params/params.html index 20edc093..276d1e15 100644 --- a/bin/report_modules/templates/params/params.html +++ b/bin/report_modules/templates/params/params.html @@ -8,7 +8,7 @@

Rashid, U., Wu, C., Shiller, J., Smith, K., Crowhurst, R., Davy, M., Chen, T.-H., Thomson, S., & Deng, C. (2024). AssemblyQC: A NextFlow pipeline for evaluating assembly quality ({{ - all_stats_dicts['VERSIONS']['SELF'] }}). Zenodo. + all_stats_dicts['VERSIONS']['Workflow']['AssemblyQC'] }}). Zenodo. 10.5281/zenodo.10647870. GitHub. https://github.com/Plant-Food-Research-Open/assembly_qchttps://github.com/tolkit/telomeric-identifier

-

Version: {{ all_stats_dicts['VERSIONS']['TIDK'] }}

+

Version: {{ all_stats_dicts['VERSIONS']['TIDK_PLOT_APRIORI']['tidk'] }}

{% include 'tidk/dropdown.html' %} {% include 'tidk/report_contents.html' %} diff --git a/bin/report_modules/templates/tools/contents.html b/bin/report_modules/templates/tools/contents.html new file mode 100644 index 00000000..721721b6 --- /dev/null +++ b/bin/report_modules/templates/tools/contents.html @@ -0,0 +1,9 @@ +
+
+
+
Pipeline Tools
+

Following is a non-exhaustive list of tools used to generate this report.

+
+ {{ all_stats_dicts['TOOLS_TABLE'] }} +
+
diff --git a/bin/report_modules/templates/tools/tools.html b/bin/report_modules/templates/tools/tools.html new file mode 100644 index 00000000..4d78232c --- /dev/null +++ b/bin/report_modules/templates/tools/tools.html @@ -0,0 +1 @@ + diff --git a/bin/reverse_edta_naming_f1b7bce.py b/bin/reverse_edta_naming_f1b7bce.py deleted file mode 100755 index c0471008..00000000 --- a/bin/reverse_edta_naming_f1b7bce.py +++ /dev/null @@ -1,48 +0,0 @@ -#!/usr/bin/env python - -import sys - -renamed_ids_tsv = sys.argv[1] -te_anno_gff3 = sys.argv[2] -intact_gff3 = sys.argv[3] -output_prefix = sys.argv[4] - - -def create_name_mapping_from_file(file_path): - dictionary = {} - - with open(file_path, "r") as tsv_file: - for line in tsv_file: - columns = line.strip().split("\t") - if len(columns) != 2: - raise ValueError(f"{file_path} should be a two column TSV file") - - orig_id, new_id = columns[0], columns[1] - dictionary[new_id] = orig_id - - return dictionary - - -def reverse_rename_gff3_file(new_to_orig_ids, file_path, output_file_name): - with open(file_path, "r") as input_gff3_file: - input_lines = input_gff3_file.readlines() - - with open(output_file_name, "w") as output_gff_file: - for line in input_lines: - if line.startswith("##"): - output_gff_file.write(line) - continue - - new_id = line.split("\t")[0] - orig_id = new_to_orig_ids[new_id] - output_gff_file.write(line.replace(new_id, orig_id)) - - -if __name__ == "__main__": - new_to_orig_ids = create_name_mapping_from_file(renamed_ids_tsv) - reverse_rename_gff3_file( - new_to_orig_ids, te_anno_gff3, f"{output_prefix}.EDTA.TEanno.gff3" - ) - reverse_rename_gff3_file( - new_to_orig_ids, intact_gff3, f"{output_prefix}.EDTA.intact.gff3" - ) diff --git a/bin/shorten_fasta_ids_c97537f.py b/bin/shorten_fasta_ids_c97537f.py deleted file mode 100755 index 1ae5076c..00000000 --- a/bin/shorten_fasta_ids_c97537f.py +++ /dev/null @@ -1,170 +0,0 @@ -#!/usr/bin/env python - -import re -import sys - -from Bio import SeqIO - -# The input fasta file path -fasta_file_path = sys.argv[1] - -# The prefix for output files: prefix.renamed.ids.fa, prefix.renamed.ids.tsv -output_files_prefix = sys.argv[2] - -# In the case where IDs have acceptable character and no change is needed, the output is stdout: -# "IDs have acceptable length and character. No change required." - - -def extract_fasta_ids(fasta_file_path): - fasta_file_obj = SeqIO.parse(fasta_file_path, "fasta") - - ids = [] - for record in fasta_file_obj: - ids.append(record.id) - return ids - - -def write_fasta_with_new_ids(fasta_file_path, id_mapping, file_prefix): - old_fasta_file_obj = SeqIO.parse(fasta_file_path, "fasta") - id_map = dict(id_mapping) - - replaced_records = [] - for record in old_fasta_file_obj: - old_id = record.id - - new_id = id_map[old_id] - record.id = new_id - record.description = "" - - replaced_records.append(record) - - SeqIO.write(replaced_records, f"{file_prefix}.renamed.ids.fa", "fasta") - - -def write_fasta_without_comments(fasta_file_path, file_prefix): - old_fasta_file_obj = SeqIO.parse(fasta_file_path, "fasta") - - replaced_records = [] - for record in old_fasta_file_obj: - record.description = "" - replaced_records.append(record) - - SeqIO.write(replaced_records, f"{file_prefix}.renamed.ids.fa", "fasta") - - -def do_id_need_to_change(id): - if len(id) > 13 or not re.match(r"^[a-zA-Z0-9_]+$", id): - return True - - return False - - -def do_ids_need_to_change(ids): - return any([do_id_need_to_change(id) for id in ids]) - - -def extract_common_patterns(ids): - pattern_counts = {} - for id in ids: - patterns = re.findall(r"[A-Za-z0_]{4,}", id) - for pattern in set(patterns): - pattern_counts[pattern] = pattern_counts.get(pattern, 0) + 1 - - common_patterns = [ - pattern for pattern, count in pattern_counts.items() if count >= 2 - ] - - if len(common_patterns) < 1: - return {} - - return {pattern: pattern[:3] for pattern in common_patterns} - - -def shorten_ids(ids, patterns_dict): - shortened_ids = [] - - for id in ids: - if not do_id_need_to_change(id): - shortened_ids.append(id) - continue - - shortened_id = shorten_id_by_pattern_replacement(patterns_dict, id) - - if not do_id_need_to_change(shortened_id): - shortened_ids.append(shortened_id) - continue - - shortened_id = f"Ctg{generate_hash(id)}" - - if not do_id_need_to_change(shortened_id): - shortened_ids.append(shortened_id) - continue - - raise ValueError(f"Failed to shorten id: {id} ({shortened_id})") - - return shortened_ids - - -def shorten_id_by_pattern_replacement(patterns_dict, id): - if patterns_dict == {}: - return id - - shortened_id = id - matches_for_id = match_substrings(patterns_dict.keys(), shortened_id) - - for pattern in matches_for_id: - shortened_id = re.sub( - r"({})".format(re.escape(pattern)), - patterns_dict[pattern], - shortened_id, - ) - return ( - shortened_id - if shortened_id[len(shortened_id) - 1] != "_" - else shortened_id[0 : (len(shortened_id) - 1)] - ) - - -def match_substrings(substrings, target_string): - pattern = "|".join(map(re.escape, substrings)) - matches = re.findall(pattern, target_string) - return matches - - -def generate_hash(string): - import hashlib - - hash_object = hashlib.sha1(string.encode()) - full_hash = hash_object.hexdigest() - short_hash = full_hash[:10] - return short_hash - - -def fail_if_new_ids_not_valid(ids): - if len(ids) != len(set(ids)): - raise ValueError("Th new IDs are not unique") - - -if __name__ == "__main__": - input_ids = extract_fasta_ids(fasta_file_path) - - if not do_ids_need_to_change(input_ids): - print("IDs have acceptable length and character. No change required.") - - with open(f"{output_files_prefix}.renamed.ids.tsv", "w") as f: - f.write("IDs have acceptable length and character. No change required.") - - write_fasta_without_comments(fasta_file_path, output_files_prefix) - - exit(0) - - new_ids = shorten_ids(input_ids, extract_common_patterns(input_ids)) - fail_if_new_ids_not_valid(new_ids) - - with open(f"{output_files_prefix}.renamed.ids.tsv", "w") as f: - for input_id, new_id in zip(input_ids, new_ids): - f.write(f"{input_id}\t{new_id}\n") - - write_fasta_with_new_ids( - fasta_file_path, zip(input_ids, new_ids), output_files_prefix - ) diff --git a/conf/base.config b/conf/base.config index 3daf70ce..ab5a46a2 100644 --- a/conf/base.config +++ b/conf/base.config @@ -79,6 +79,10 @@ process { withLabel:process_very_high_memory { memory = { check_max( 512.GB * task.attempt, 'memory' ) } } + + withName:CUSTOM_DUMPSOFTWAREVERSIONS { + cache = false + } } // Source: https://github.com/nf-core/rnaseq diff --git a/conf/modules.config b/conf/modules.config index 71a2e696..0d1d111b 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -1,151 +1,220 @@ -if(!params.tidk.skip) { - process { - - withName: FILTER_BY_LENGTH { - ext.args = params.tidk.filter_by_size ? "-m ${params.tidk.filter_size_bp}" : '' - ext.prefix = { "${meta.id}.filtered" } - } - - withName: SORT_BY_LENGTH { - ext.args = '--quiet --reverse --by-length' - ext.prefix = { "${meta.id}.sorted" } - } - - withName: TIDK_EXPLORE { - ext.args = '--minimum 5 --maximum 30' - publishDir = [ - path: { "${params.outdir}/tidk" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } - ] - } - - withName: TIDK_SEARCH_APRIORI { - ext.prefix = { "${meta.id}.apriori" } - ext.args = '--extension tsv' - publishDir = [ - path: { "${params.outdir}/tidk" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } - ] - } - - withName: TIDK_SEARCH_APOSTERIORI { - ext.prefix = { "${meta.id}.aposteriori" } - ext.args = '--extension tsv' - publishDir = [ - path: { "${params.outdir}/tidk" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } - ] - } - - withName: TIDK_PLOT_APRIORI { - ext.prefix = { "${meta.id}.apriori" } - publishDir = [ - path: { "${params.outdir}/tidk" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } - ] - } - - withName: TIDK_PLOT_APOSTERIORI { - ext.prefix = { "${meta.id}.aposteriori" } - publishDir = [ - path: { "${params.outdir}/tidk" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } - ] - } +process { + withName: ASSEMBLATHON_STATS { + publishDir = [ + path: { "${params.outdir}/assemblathon_stats" }, + mode: "copy", + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: NCBI_FCS_ADAPTOR { + publishDir = [ + path: { "${params.outdir}/ncbi_fcs_adaptor" }, + mode: "copy", + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: RUN_BUSCO { + publishDir = [ + path: { "${params.outdir}/busco" }, + mode: "copy", + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: RUN_KRAKEN2 { + publishDir = [ + path: { "${params.outdir}/kraken2" }, + mode: "copy", + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: '.*:NCBI_FCS_GX:SCREEN_SAMPLES' { + publishDir = [ + path: { "${params.outdir}/ncbi_fcs_gx" }, + mode: "copy", + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: CIRCOS { + publishDir = [ + path: { "${params.outdir}/synteny/${target_on_ref_seq}" }, + mode: "copy", + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } +} + + +process { + withName: '.*:GFF3_VALIDATE:GT_GFF3' { + ext.args = '-tidy -retainids' + } + + withName: GT_STAT { + ext.args = [ + '-genelengthdistri', + '-genescoredistri', + '-exonlengthdistri', + '-exonnumberdistri', + '-intronlengthdistri', + '-cdslengthdistri', + '-addintrons' + ].join(' ').trim() + + publishDir = [ + path: { "${params.outdir}/genometools_gt_stat" }, + mode: "copy", + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } +} + +process { + + withName: FILTER_BY_LENGTH { + ext.args = params.tidk.filter_by_size ? "-m ${params.tidk.filter_size_bp}" : '' + ext.prefix = { "${meta.id}.filtered" } + } + + withName: SORT_BY_LENGTH { + ext.args = '--quiet --reverse --by-length' + ext.prefix = { "${meta.id}.sorted" } + } + + withName: TIDK_EXPLORE { + ext.args = '--minimum 5 --maximum 30' + publishDir = [ + path: { "${params.outdir}/tidk" }, + mode: "copy", + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: TIDK_SEARCH_APRIORI { + ext.prefix = { "${meta.id}.apriori" } + ext.args = '--extension tsv' + publishDir = [ + path: { "${params.outdir}/tidk" }, + mode: "copy", + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: TIDK_SEARCH_APOSTERIORI { + ext.prefix = { "${meta.id}.aposteriori" } + ext.args = '--extension tsv' + publishDir = [ + path: { "${params.outdir}/tidk" }, + mode: "copy", + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: TIDK_PLOT_APRIORI { + ext.prefix = { "${meta.id}.apriori" } + publishDir = [ + path: { "${params.outdir}/tidk" }, + mode: "copy", + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: TIDK_PLOT_APOSTERIORI { + ext.prefix = { "${meta.id}.aposteriori" } + publishDir = [ + path: { "${params.outdir}/tidk" }, + mode: "copy", + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] } } -if(!params.lai.skip) { - process { - - withName: CUSTOM_SHORTENFASTAIDS { - publishDir = [ - path: { "${params.outdir}/lai" }, - mode: "copy", - pattern: '*.short.ids.tsv' - ] - } - - withName: EDTA_LTRHARVEST { - ext.prefix = { "${meta.id}_edta_ltrharvest" } - } - - withName: LTRFINDER { - ext.args = '-harvest_out -size 1000000 -time 300' - } - - withName: CAT_CAT { - ext.prefix = { "${meta.id}_ltrharvest_ltrfinder.tabout" } - } - - withName: LTRRETRIEVER { - publishDir = [ - path: { "${params.outdir}/lai" }, - mode: "copy", - pattern: '*.LTRlib.fa' - ] - } - - withName: CUSTOM_RESTOREGFFIDS { - publishDir = [ - path: { "${params.outdir}/lai" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } - ] - } - - withName: LAI { - publishDir = [ - path: { "${params.outdir}/lai" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } - ] - } +process { + + withName: CUSTOM_SHORTENFASTAIDS { + publishDir = [ + path: { "${params.outdir}/lai" }, + mode: "copy", + pattern: '*.short.ids.tsv' + ] + } + + withName: EDTA_LTRHARVEST { + ext.prefix = { "${meta.id}_edta_ltrharvest" } + } + + withName: LTRFINDER { + ext.args = '-harvest_out -size 1000000 -time 300' + } + + withName: CAT_CAT { + ext.prefix = { "${meta.id}_ltrharvest_ltrfinder.tabout" } + } + + withName: LTRRETRIEVER { + publishDir = [ + path: { "${params.outdir}/lai" }, + mode: "copy", + pattern: '*.LTRlib.fa' + ] + } + + withName: CUSTOM_RESTOREGFFIDS { + publishDir = [ + path: { "${params.outdir}/lai" }, + mode: "copy", + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: LAI { + publishDir = [ + path: { "${params.outdir}/lai" }, + mode: "copy", + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] } } -if(!params.hic.skip){ - process { - - withName: FASTQC_RAW { - publishDir = [ - path: { "${params.outdir}/hic/fastqc_raw" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } - ] - } - - withName: FASTQC_TRIM { - publishDir = [ - path: { "${params.outdir}/hic/fastqc_trim" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } - ] - } - - withName: FASTP { - ext.args = params.hic.fastp_ext_args - publishDir = [ - path: { "${params.outdir}/hic/fastp" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } - ] - } - - withName: BWA_MEM { - ext.prefix = { "${meta.id}.on.${meta.ref_id}.bwa.mem" } - ext.args = '-5SP' - } - - withName: SAMBLASTER { - ext.prefix = { "${meta.id}.on.${meta.ref_id}.samblaster" } - ext.args3 = '-h -F 2316' - } +process { + + withName: FASTQC_RAW { + publishDir = [ + path: { "${params.outdir}/hic/fastqc_raw" }, + mode: "copy", + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: FASTQC_TRIM { + publishDir = [ + path: { "${params.outdir}/hic/fastqc_trim" }, + mode: "copy", + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: FASTP { + ext.args = params.hic.fastp_ext_args + publishDir = [ + path: { "${params.outdir}/hic/fastp" }, + mode: "copy", + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: BWA_MEM { + ext.prefix = { "${meta.id}.on.${meta.ref_id}.bwa.mem" } + ext.args = '-5SP' + } + + withName: SAMBLASTER { + ext.prefix = { "${meta.id}.on.${meta.ref_id}.samblaster" } + ext.args3 = '-h -F 2316' } } diff --git a/conf/test_invalid.config b/conf/test_invalid.config new file mode 100644 index 00000000..6af810e6 --- /dev/null +++ b/conf/test_invalid.config @@ -0,0 +1,40 @@ +params { + target_assemblies = [ + [ + "FI1", + "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz" + ], + [ + "FI2", + "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz" + ] + ] + + assembly_gff3 = [ + [ + "FI1", + "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz" + ], + [ + "FI2", + "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/196/095/GCF_000196095.1_ASM19609v1/GCF_000196095.1_ASM19609v1_genomic.gff.gz" + ], + ] + + ncbi_fcs_adaptor { skip = 1 } + ncbi_fcs_gx { skip = 1 } + busco { skip = 1 } + tidk { skip = 1 } + lai { skip = 1 } + kraken2 { skip = 1 } + hic { skip = 1 } + synteny { skip = 1 } + + outdir = "./results" + + max_time = 1.hour +} + +apptainer { + cacheDir = "/workspace/assembly_qc/singularity" +} diff --git a/modules.json b/modules.json index be08b97d..67f866b4 100644 --- a/modules.json +++ b/modules.json @@ -20,6 +20,11 @@ "git_sha": "a2c6aa54917a232ac543d88edc5d3c3d8bf71ef3", "installed_by": ["fasta_ltrretriever_lai"] }, + "custom/checkgff3fastacorrespondence": { + "branch": "main", + "git_sha": "1a76f884082c786760559c462063a5d1de94ca83", + "installed_by": ["gff3_validate"] + }, "custom/restoregffids": { "branch": "main", "git_sha": "e9f6bdd634bdbcd52c5568ba82f16176ec06631f", @@ -35,6 +40,21 @@ "git_sha": "5a3fa7e2643328a95ced3632b761edea710abe07", "installed_by": ["fasta_ltrretriever_lai"] }, + "gt/gff3": { + "branch": "main", + "git_sha": "bfa4874d3942bdff70cb8df17322834125cafb28", + "installed_by": ["gff3_validate"] + }, + "gt/gff3validator": { + "branch": "main", + "git_sha": "889b9b57b611dcb063594608c2a222c928327cba", + "installed_by": ["gff3_validate"] + }, + "gt/stat": { + "branch": "main", + "git_sha": "cb5fb0be78a98fd1e32b7c90d6adf8c3bf44133e", + "installed_by": ["modules"] + }, "lai": { "branch": "main", "git_sha": "7e6e3cb41362a045c6bb6065903efa0eba246e87", @@ -68,6 +88,11 @@ "branch": "main", "git_sha": "9639ac9a556898d0f0e8592bff24585c33326458", "installed_by": ["subworkflows"] + }, + "gff3_validate": { + "branch": "main", + "git_sha": "3fa824f807c433eebd154a424c2e50e1dae7b050", + "installed_by": ["subworkflows"] } } } @@ -75,6 +100,16 @@ "https://github.com/nf-core/modules.git": { "modules": { "nf-core": { + "custom/dumpsoftwareversions": { + "branch": "master", + "git_sha": "de45447d060b8c8b98575bc637a4a575fd0638e1", + "installed_by": ["modules"] + }, + "fastavalidator": { + "branch": "master", + "git_sha": "89ff95427f695086369d7927a3c17cea2a37a382", + "installed_by": ["modules"] + }, "fastp": { "branch": "master", "git_sha": "003920c7f9a8ae19b69a97171922880220bedf56", @@ -85,6 +120,11 @@ "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", "installed_by": ["fastq_trim_fastp_fastqc"] }, + "gunzip": { + "branch": "master", + "git_sha": "3a5fef109d113b4997c9822198664ca5f2716208", + "installed_by": ["modules"] + }, "seqkit/seq": { "branch": "master", "git_sha": "687ad41c14008d3d55cf7c2ffacebe6a057211a4", diff --git a/modules/local/agp2_assembly.nf b/modules/local/agp2_assembly.nf index b010c712..b78450ac 100644 --- a/modules/local/agp2_assembly.nf +++ b/modules/local/agp2_assembly.nf @@ -1,5 +1,3 @@ -nextflow.enable.dsl=2 - process AGP2_ASSEMBLY { tag "$sample_id_on_tag" label "process_single" @@ -8,14 +6,14 @@ process AGP2_ASSEMBLY { publishDir "${params.outdir}/hic/assembly", mode:'copy' input: - tuple val(sample_id_on_tag), path(agp_file) + tuple val(sample_id_on_tag), path(agp_file) output: - tuple val(sample_id_on_tag), path("*.agp.assembly"), emit: agp_assembly_file + tuple val(sample_id_on_tag), path("*.agp.assembly"), emit: assembly script: - """ - assembly_tag=\$(echo $sample_id_on_tag | sed 's/.*\\.on\\.//g') - agp2assembly.py $agp_file "\${assembly_tag}.agp.assembly" - """ + """ + assembly_tag=\$(echo $sample_id_on_tag | sed 's/.*\\.on\\.//g') + agp2assembly.py $agp_file "\${assembly_tag}.agp.assembly" + """ } diff --git a/modules/local/assemblathon_stats.nf b/modules/local/assemblathon_stats.nf index 7acd55b9..edd85107 100644 --- a/modules/local/assemblathon_stats.nf +++ b/modules/local/assemblathon_stats.nf @@ -1,6 +1,3 @@ -nextflow.enable.dsl=2 - - process ASSEMBLATHON_STATS { tag "${hap_name}" label "process_single" @@ -9,32 +6,38 @@ process ASSEMBLATHON_STATS { 'https://depot.galaxyproject.org/singularity/ubuntu:20.04': 'quay.io/nf-core/ubuntu:20.04' }" - publishDir "${params.outdir}/assemblathon_stats", mode: 'copy' - input: - tuple val(hap_name), path(fasta_file) + tuple val(hap_name), path(fasta_file) output: - path "${hap_name}_stats.csv" + path "${hap_name}_stats.csv" , emit: stats + path 'versions.yml' , emit: versions script: - """ - paths_to_check=\$(printf "%s\\n" \$(echo \$PATH | tr ':' ' ') \ - | xargs -I {} find {} -maxdepth 0 -print 2>/dev/null \ - | grep -v '^\$' \ - | grep -v '/sbin' \ - | xargs) - - falite_path="\$(find \$paths_to_check -name FAlite_943e0fb.pm)" - - ln -s "\$falite_path" FAlite_943e0fb.pm - - PERL5LIB=./ assemblathon_stats_943e0fb.pl \ - -n ${params.assemblathon_stats.n_limit} \ - -csv \ + def VERSION = "github/PlantandFoodResearch/assemblathon2-analysis/a93cba2" + """ + paths_to_check=\$(printf "%s\\n" \$(echo \$PATH | tr ':' ' ') \\ + | xargs -I {} find {} -maxdepth 0 -print 2>/dev/null \\ + | grep -v '^\$' \\ + | grep -v '/sbin' \\ + | xargs + ) + + falite_path="\$(find \$paths_to_check -name FAlite_943e0fb.pm)" + + ln -s "\$falite_path" FAlite_943e0fb.pm + + PERL5LIB=./ assemblathon_stats_943e0fb.pl \\ + -n ${params.assemblathon_stats.n_limit} \\ + -csv \\ "${fasta_file}" - csv_file_name=\$(ls | grep "csv") - mv \$csv_file_name "${hap_name}_stats.csv" - """ + csv_file_name=\$(ls | grep "csv") + mv \$csv_file_name "${hap_name}_stats.csv" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + assemblathon_stats: $VERSION + END_VERSIONS + """ } diff --git a/modules/local/assembly2_bedpe.nf b/modules/local/assembly2_bedpe.nf index d2c05113..9d117e50 100644 --- a/modules/local/assembly2_bedpe.nf +++ b/modules/local/assembly2_bedpe.nf @@ -1,5 +1,3 @@ -nextflow.enable.dsl=2 - process ASSEMBLY2_BEDPE { tag "$sample_id_on_tag" label "process_single" diff --git a/modules/local/biocode_gff3_stats.nf b/modules/local/biocode_gff3_stats.nf deleted file mode 100644 index 9f52aba6..00000000 --- a/modules/local/biocode_gff3_stats.nf +++ /dev/null @@ -1,22 +0,0 @@ -nextflow.enable.dsl=2 - -process BIOCODE_GFF3_STATS { - tag "${tag_label}" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/biocode:0.10.0--pyhdfd78af_0': - 'quay.io/biocontainers/biocode:0.10.0--pyhdfd78af_0' }" - publishDir "${params.outdir}/biocode_gff3_stats", mode: 'copy' - - input: - tuple val(tag_label), path(gff3_file) - - output: - path "${tag_label}_stats.csv" - - script: - """ - report_gff3_statistics.py --input_file "$gff3_file" &>> "${tag_label}_stats.csv" || true - """ -} diff --git a/modules/local/create_report.nf b/modules/local/create_report.nf index 703e375a..6a9fd169 100644 --- a/modules/local/create_report.nf +++ b/modules/local/create_report.nf @@ -1,33 +1,33 @@ -nextflow.enable.dsl=2 - process CREATE_REPORT { - tag "all modules" + tag "AssemblyQC" label "process_single" - container "docker.io/gallvp/python3npkgs:v0.4" + // container "docker.io/gallvp/python3npkgs:v0.4" publishDir params.outdir, mode: 'copy' input: - path ncbi_fcs_adaptor_reports, stageAs: 'ncbi_fcs_adaptor_reports/*' - path fcs_gx_reports, stageAs: 'fcs_gx_reports/*' - path assemblathon_stats, stageAs: 'assemblathon_stats/*' - path genometools_gt_stats, stageAs: 'genometools_gt_stat/*' - path biocode_gff3_stats, stageAs: 'biocode_gff3_stats/*' - path busco_outputs, stageAs: 'busco_outputs/*' - path tidk_plots, stageAs: 'tidk_outputs/*' - path lai_outputs, stageAs: 'lai_outputs/*' - path kraken2_outputs, stageAs: 'kraken2_outputs/*' - path hic_outputs, stageAs: 'hic_outputs/*' - path circos_outputs, stageAs: 'circos_outputs/*' - val params_json + path fastavalidator_logs, stageAs: 'fastavalidator_logs/*' + path gff3_validate_logs, stageAs: 'gff3_validate_logs/*' + path ncbi_fcs_adaptor_reports, stageAs: 'ncbi_fcs_adaptor_reports/*' + path fcs_gx_reports, stageAs: 'fcs_gx_reports/*' + path assemblathon_stats, stageAs: 'assemblathon_stats/*' + path genometools_gt_stats, stageAs: 'genometools_gt_stat/*' + path busco_outputs, stageAs: 'busco_outputs/*' + path tidk_plots, stageAs: 'tidk_outputs/*' + path lai_outputs, stageAs: 'lai_outputs/*' + path kraken2_outputs, stageAs: 'kraken2_outputs/*' + path hic_outputs, stageAs: 'hic_outputs/*' + path circos_outputs, stageAs: 'circos_outputs/*' + path versions + val params_json output: - path 'report.html' - path 'report.json' + path 'report.html' + path 'report.json' script: - """ - echo -n '$params_json' > params_json.json - assembly_qc_report_943e0fb.py > report.html - """ + """ + echo -n '$params_json' > params_json.json + assembly_qc_report_943e0fb.py > report.html + """ } diff --git a/modules/local/genometools_gt_stat.nf b/modules/local/genometools_gt_stat.nf deleted file mode 100644 index e7bd29c1..00000000 --- a/modules/local/genometools_gt_stat.nf +++ /dev/null @@ -1,24 +0,0 @@ -nextflow.enable.dsl=2 - - -process GENOMETOOLS_GT_STAT { - tag "${hap_name}" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/genometools-genometools:1.6.2--py310he7ef181_3': - 'quay.io/biocontainers/genometools-genometools:1.6.2--py310he7ef181_3' }" - - publishDir "${params.outdir}/genometools_gt_stat", mode: 'copy' - - input: - tuple val(hap_name), path(gff3_file) - - output: - path "${hap_name}_stats.csv" - - script: - """ - gt stat "${gff3_file}" | sed 's/:/,/1' > "${hap_name}_stats.csv" - """ -} diff --git a/modules/local/gzip_fasta.nf b/modules/local/gzip_fasta.nf deleted file mode 100644 index 747a0ce1..00000000 --- a/modules/local/gzip_fasta.nf +++ /dev/null @@ -1,24 +0,0 @@ -nextflow.enable.dsl=2 - -process GZIP_FASTA { - tag "${tag_label}" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/ubuntu:20.04': - 'quay.io/nf-core/ubuntu:20.04' }" - - input: - tuple val(tag_label), path(fasta_file) - - output: - tuple val(tag_label), path("*.gzip.fa") - - script: - """ - input_file_name_var="\$(basename $fasta_file .gz)" - output_file_name="\${input_file_name_var%.*}.gzip.fa" - - gzip -cdf $fasta_file > \$output_file_name - """ -} diff --git a/modules/local/hic_qc.nf b/modules/local/hic_qc.nf index b2f63f31..cc87c68c 100644 --- a/modules/local/hic_qc.nf +++ b/modules/local/hic_qc.nf @@ -1,5 +1,3 @@ -nextflow.enable.dsl=2 - process HIC_QC { tag "$sample_id_on_tag" label "process_single" diff --git a/modules/local/juicer_sort.nf b/modules/local/juicer_sort.nf index 4d7c15d1..c9b0577f 100644 --- a/modules/local/juicer_sort.nf +++ b/modules/local/juicer_sort.nf @@ -1,5 +1,3 @@ -nextflow.enable.dsl=2 - process JUICER_SORT { tag "$sample_id_on_tag" label "process_high" @@ -9,16 +7,16 @@ process JUICER_SORT { 'quay.io/nf-core/ubuntu:20.04' }" input: - tuple val(sample_id_on_tag), path(out_links_txt) + tuple val(sample_id_on_tag), path(out_links_txt) output: - tuple val(sample_id_on_tag), path("*sorted.links.txt"), emit: sorted_links_txt_file + tuple val(sample_id_on_tag), path("*sorted.links.txt"), emit: links script: - """ - sort --parallel=${task.cpus} \ - -k2,2 -k6,6 \ - $out_links_txt \ + """ + sort --parallel=${task.cpus} \\ + -k2,2 -k6,6 \\ + $out_links_txt \\ > out.sorted.links.txt - """ + """ } diff --git a/modules/local/make_agp_from_fasta.nf b/modules/local/make_agp_from_fasta.nf index 7734eed7..6541b3e5 100644 --- a/modules/local/make_agp_from_fasta.nf +++ b/modules/local/make_agp_from_fasta.nf @@ -1,5 +1,3 @@ -nextflow.enable.dsl=2 - process MAKE_AGP_FROM_FASTA { tag "$sample_id_on_tag" label "process_single" diff --git a/modules/local/matlock_bam2_juicer.nf b/modules/local/matlock_bam2_juicer.nf index a37f7bd1..4432f0c9 100644 --- a/modules/local/matlock_bam2_juicer.nf +++ b/modules/local/matlock_bam2_juicer.nf @@ -1,5 +1,3 @@ -nextflow.enable.dsl=2 - process MATLOCK_BAM2_JUICER { tag "$sample_id_on_tag" label "process_single" diff --git a/modules/local/ncbi_fcs_adaptor.nf b/modules/local/ncbi_fcs_adaptor.nf new file mode 100644 index 00000000..9e14c0c5 --- /dev/null +++ b/modules/local/ncbi_fcs_adaptor.nf @@ -0,0 +1,34 @@ +process NCBI_FCS_ADAPTOR { + tag "${hap_name}" + label "process_single" + + container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + 'https://ftp.ncbi.nlm.nih.gov/genomes/TOOLS/FCS/releases/0.4.0/fcs-adaptor.sif': + 'docker.io/ncbi/fcs-adaptor:0.4.0' }" + + input: + tuple val(hap_name), path(fasta_file) + + output: + tuple val(hap_name), path("${hap_name}_fcs_adaptor_report.tsv") , emit: report + path "versions.yml" , emit: versions + + script: + def VERSION = 0.4 + """ + mkdir "${hap_name}_outputdir" + + /app/fcs/bin/av_screen_x \\ + -o "${hap_name}_outputdir" \\ + --${params.ncbi_fcs_adaptor.empire} \\ + "${fasta_file}" + + mv "${hap_name}_outputdir/fcs_adaptor_report.txt" \\ + "./${hap_name}_fcs_adaptor_report.tsv" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + av_screen_x: $VERSION + END_VERSIONS + """ +} diff --git a/modules/local/run_assembly_visualizer.nf b/modules/local/run_assembly_visualizer.nf index e0cfac8e..3f44a168 100644 --- a/modules/local/run_assembly_visualizer.nf +++ b/modules/local/run_assembly_visualizer.nf @@ -1,5 +1,3 @@ -nextflow.enable.dsl=2 - process RUN_ASSEMBLY_VISUALIZER { tag "$sample_id_on_tag" label "process_medium" @@ -8,17 +6,20 @@ process RUN_ASSEMBLY_VISUALIZER { container "gallvp/3d-dna:63029aa" input: - tuple val(sample_id_on_tag), path(agp_assembly_file), path(sorted_links_txt_file) + tuple val(sample_id_on_tag), path(agp_assembly_file), path(sorted_links_txt_file) output: - tuple val(sample_id_on_tag), path("*.hic"), emit: hic_file + tuple val(sample_id_on_tag), path("*.hic"), emit: hic script: - // -p true/false Use GNU Parallel to speed up computation (default is true). - """ - assembly_tag=\$(echo $sample_id_on_tag | sed 's/.*\\.on\\.//g') - file_name="${agp_assembly_file}" - /usr/src/3d-dna/visualize/run-assembly-visualizer.sh $agp_assembly_file $sorted_links_txt_file - mv "\${file_name%.*}.hic" "\${assembly_tag}.hic" - """ + // -p true/false Use GNU Parallel to speed up computation (default is true). + """ + assembly_tag=\$(echo $sample_id_on_tag | sed 's/.*\\.on\\.//g') + file_name="${agp_assembly_file}" + + /usr/src/3d-dna/visualize/run-assembly-visualizer.sh \\ + $agp_assembly_file $sorted_links_txt_file + + mv "\${file_name%.*}.hic" "\${assembly_tag}.hic" + """ } diff --git a/modules/local/utils.nf b/modules/local/utils.nf index 9075beb0..c3d3d8ea 100644 --- a/modules/local/utils.nf +++ b/modules/local/utils.nf @@ -1,5 +1,3 @@ -nextflow.enable.dsl=2 - import groovy.json.JsonOutput def jsonifyParams(params) { diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml new file mode 100644 index 00000000..b48ced26 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -0,0 +1,7 @@ +name: custom_dumpsoftwareversions +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::multiqc=1.20 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf new file mode 100644 index 00000000..27f7f11b --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -0,0 +1,24 @@ +process CUSTOM_DUMPSOFTWAREVERSIONS { + label 'process_single' + + // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + 'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' : + 'quay.io/biocontainers/multiqc:1.20--pyhdfd78af_0' }" + + input: + path versions + + output: + path "software_versions.yml" , emit: yml + path "software_versions_mqc.yml", emit: mqc_yml + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + template 'dumpsoftwareversions.py' +} diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml new file mode 100644 index 00000000..5f15a5fd --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/meta.yml @@ -0,0 +1,37 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: custom_dumpsoftwareversions +description: Custom module used to dump software versions within the nf-core pipeline template +keywords: + - custom + - dump + - version +tools: + - custom: + description: Custom module used to dump software versions within the nf-core pipeline template + homepage: https://github.com/nf-core/tools + documentation: https://github.com/nf-core/tools + licence: ["MIT"] +input: + - versions: + type: file + description: YML file containing software versions + pattern: "*.yml" +output: + - yml: + type: file + description: Standard YML file containing software versions + pattern: "software_versions.yml" + - mqc_yml: + type: file + description: MultiQC custom content YML file containing software versions + pattern: "software_versions_mqc.yml" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" + - "@grst" +maintainers: + - "@drpatelh" + - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py new file mode 100755 index 00000000..4a993608 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py @@ -0,0 +1,103 @@ +#!/usr/bin/env python + + +"""Provide functions to merge multiple versions.yml files.""" + + +import yaml +import platform +from textwrap import dedent + + +def _make_versions_html(versions): + """Generate a tabular HTML output of all versions for MultiQC.""" + html = [ + dedent( + """\\ + + + + + + + + + + """ + ) + ] + for process, tmp_versions in sorted(versions.items()): + html.append("") + for i, (tool, version) in enumerate(sorted(tmp_versions.items())): + html.append( + dedent( + f"""\\ + + + + + + """ + ) + ) + html.append("") + html.append("
Process Name Software Version
{process if (i == 0) else ''}{tool}{version}
") + return "\\n".join(html) + + +def main(): + """Load all version files and generate merged output.""" + versions_this_module = {} + versions_this_module["${task.process}"] = { + "python": platform.python_version(), + "yaml": yaml.__version__, + } + + with open("$versions") as f: + versions_by_process = ( + yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module + ) + + # aggregate versions by the module name (derived from fully-qualified process name) + versions_by_module = {} + for process, process_versions in versions_by_process.items(): + module = process.split(":")[-1] + try: + if versions_by_module[module] != process_versions: + raise AssertionError( + "We assume that software versions are the same between all modules. " + "If you see this error-message it means you discovered an edge-case " + "and should open an issue in nf-core/tools. " + ) + except KeyError: + versions_by_module[module] = process_versions + + versions_by_module["Workflow"] = { + "Nextflow": "$workflow.nextflow.version", + "$workflow.manifest.name": "$workflow.manifest.version", + } + + versions_mqc = { + "id": "software_versions", + "section_name": "${workflow.manifest.name} Software Versions", + "section_href": "https://github.com/${workflow.manifest.name}", + "plot_type": "html", + "description": "are collected at run time from the software output.", + "data": _make_versions_html(versions_by_module), + } + + with open("software_versions.yml", "w") as f: + yaml.dump(versions_by_module, f, default_flow_style=False) + with open("software_versions_mqc.yml", "w") as f: + yaml.dump(versions_mqc, f, default_flow_style=False) + + with open("versions.yml", "w") as f: + yaml.dump(versions_this_module, f, default_flow_style=False) + + +if __name__ == "__main__": + main() diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test new file mode 100644 index 00000000..2455d302 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test @@ -0,0 +1,43 @@ +nextflow_process { + + name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS" + script "../main.nf" + process "CUSTOM_DUMPSOFTWAREVERSIONS" + tag "modules" + tag "modules_nfcore" + tag "custom" + tag "dumpsoftwareversions" + tag "custom/dumpsoftwareversions" + + test("Should run without failures") { + when { + process { + """ + def tool1_version = ''' + TOOL1: + tool1: 0.11.9 + '''.stripIndent() + + def tool2_version = ''' + TOOL2: + tool2: 1.9 + '''.stripIndent() + + input[0] = Channel.of(tool1_version, tool2_version).collectFile() + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.versions, + file(process.out.mqc_yml[0]).readLines()[0..10], + file(process.out.yml[0]).readLines()[0..7] + ).match() + } + ) + } + } +} \ No newline at end of file diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap new file mode 100644 index 00000000..5f59a936 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap @@ -0,0 +1,33 @@ +{ + "Should run without failures": { + "content": [ + [ + "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" + ], + [ + "data: \"\\n\\n \\n \\n \\n \\n \\n \\n \\n\\", + " \\n\\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n \\n \\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n\\n\\n \\n\\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\" + ], + [ + "CUSTOM_DUMPSOFTWAREVERSIONS:", + " python: 3.11.7", + " yaml: 5.4.1", + "TOOL1:", + " tool1: 0.11.9", + "TOOL2:", + " tool2: '1.9'", + "Workflow:" + ] + ], + "timestamp": "2024-01-09T23:01:18.710682" + } +} \ No newline at end of file diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml new file mode 100644 index 00000000..405aa24a --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml @@ -0,0 +1,2 @@ +custom/dumpsoftwareversions: + - modules/nf-core/custom/dumpsoftwareversions/** diff --git a/modules/nf-core/fastavalidator/environment.yml b/modules/nf-core/fastavalidator/environment.yml new file mode 100644 index 00000000..70f346ef --- /dev/null +++ b/modules/nf-core/fastavalidator/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "fastavalidator" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::py_fasta_validator=0.6" diff --git a/modules/nf-core/fastavalidator/main.nf b/modules/nf-core/fastavalidator/main.nf new file mode 100644 index 00000000..8371dd6f --- /dev/null +++ b/modules/nf-core/fastavalidator/main.nf @@ -0,0 +1,62 @@ +process FASTAVALIDATOR { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + 'https://depot.galaxyproject.org/singularity/py_fasta_validator:0.6--py37h595c7a6_0': + 'quay.io/biocontainers/py_fasta_validator:0.6--py37h595c7a6_0' }" + + input: + tuple val(meta), path(fasta) + + output: + tuple val(meta), path('*.success.log') , emit: success_log , optional: true + tuple val(meta), path('*.error.log') , emit: error_log , optional: true + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + py_fasta_validator \\ + -f $fasta \\ + 2> "${prefix}.error.log" \\ + || echo "Errors from fasta_validate printed to ${prefix}.error.log" + + if [ \$(cat "${prefix}.error.log" | wc -l) -gt 0 ]; then + echo "Validation failed..." + + cat \\ + "${prefix}.error.log" + else + echo "Validation successful..." + + mv \\ + "${prefix}.error.log" \\ + fasta_validate.stderr + + echo "Validation successful..." \\ + > "${prefix}.success.log" + fi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + py_fasta_validator: \$(py_fasta_validator -v | sed 's/.* version //') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + echo "Validation successful..." \\ + > "${prefix}.success.log" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + py_fasta_validator: \$(py_fasta_validator -v | sed 's/.* version //') + END_VERSIONS + """ +} diff --git a/modules/nf-core/fastavalidator/meta.yml b/modules/nf-core/fastavalidator/meta.yml new file mode 100644 index 00000000..c5c4371c --- /dev/null +++ b/modules/nf-core/fastavalidator/meta.yml @@ -0,0 +1,53 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "fastavalidator" +description: | + "Python C-extension for a simple validator for fasta files. The module emits the validated file or an + error log upon validation failure." +keywords: + - fasta + - validation + - genome +tools: + - fasta_validate: + description: | + "Python C-extension for a simple C code to validate a fasta file. It only checks a few things, + and by default only sets its response via the return code, + so you will need to check that!" + homepage: "https://github.com/linsalrob/py_fasta_validator" + documentation: "https://github.com/linsalrob/py_fasta_validator" + tool_dev_url: "https://github.com/linsalrob/py_fasta_validator" + doi: "10.5281/zenodo.5002710" + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing file information + e.g. [ id:'test' ] + - fasta: + type: file + description: Input fasta file + pattern: "*.fasta" +output: + - meta: + type: map + description: | + Groovy Map containing file information + e.g. [ id:'test' ] + - success_log: + type: file + description: Log file for successful validation + pattern: "*.success.log" + - error_log: + type: file + description: Log file for failed validation + pattern: "*.error.log" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@gallvp" +maintainers: + - "@gallvp" diff --git a/modules/nf-core/fastavalidator/tests/main.nf.test b/modules/nf-core/fastavalidator/tests/main.nf.test new file mode 100644 index 00000000..52766f49 --- /dev/null +++ b/modules/nf-core/fastavalidator/tests/main.nf.test @@ -0,0 +1,60 @@ +nextflow_process { + + name "Test Process FASTAVALIDATOR" + script "../main.nf" + process "FASTAVALIDATOR" + + tag "modules" + tag "modules_nfcore" + tag "fastavalidator" + + test("sarscov2-fasta-valid") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.success_log != null }, + { assert process.out.error_log == [] }, + { assert path(process.out.success_log.get(0).get(1)).getText().contains("Validation successful...") } + ) + } + + } + + test("sarscov2-gff3-invalid") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.success_log == [] }, + { assert process.out.error_log != null }, + { assert path(process.out.error_log.get(0).get(1)).getText().contains("genome.gff3 does not start with a >") } + ) + } + + } +} \ No newline at end of file diff --git a/modules/nf-core/fastavalidator/tests/main.nf.test.snap b/modules/nf-core/fastavalidator/tests/main.nf.test.snap new file mode 100644 index 00000000..382dee72 --- /dev/null +++ b/modules/nf-core/fastavalidator/tests/main.nf.test.snap @@ -0,0 +1,76 @@ +{ + "sarscov2-fasta-valid": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.success.log:md5,b0b859eda1db5cd43915846e00ebc22c" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,05aa059840b3b4dd6d88bc1e4bf976d7" + ], + "error_log": [ + + ], + "success_log": [ + [ + { + "id": "test", + "single_end": false + }, + "test.success.log:md5,b0b859eda1db5cd43915846e00ebc22c" + ] + ], + "versions": [ + "versions.yml:md5,05aa059840b3b4dd6d88bc1e4bf976d7" + ] + } + ], + "timestamp": "2023-11-28T11:23:25.106872" + }, + "sarscov2-gff3-invalid": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test.error.log:md5,531d520c0e7767176f743f197f1f87b3" + ] + ], + "2": [ + "versions.yml:md5,05aa059840b3b4dd6d88bc1e4bf976d7" + ], + "error_log": [ + [ + { + "id": "test", + "single_end": false + }, + "test.error.log:md5,531d520c0e7767176f743f197f1f87b3" + ] + ], + "success_log": [ + + ], + "versions": [ + "versions.yml:md5,05aa059840b3b4dd6d88bc1e4bf976d7" + ] + } + ], + "timestamp": "2023-11-28T11:23:29.40324" + } +} \ No newline at end of file diff --git a/modules/nf-core/fastavalidator/tests/tags.yml b/modules/nf-core/fastavalidator/tests/tags.yml new file mode 100644 index 00000000..c3c77576 --- /dev/null +++ b/modules/nf-core/fastavalidator/tests/tags.yml @@ -0,0 +1,2 @@ +fastavalidator: + - "modules/nf-core/fastavalidator/**" diff --git a/modules/nf-core/gunzip/environment.yml b/modules/nf-core/gunzip/environment.yml new file mode 100644 index 00000000..25910b34 --- /dev/null +++ b/modules/nf-core/gunzip/environment.yml @@ -0,0 +1,7 @@ +name: gunzip +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - conda-forge::sed=4.7 diff --git a/modules/nf-core/gunzip/main.nf b/modules/nf-core/gunzip/main.nf new file mode 100644 index 00000000..aea95e75 --- /dev/null +++ b/modules/nf-core/gunzip/main.nf @@ -0,0 +1,48 @@ +process GUNZIP { + tag "$archive" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : + 'quay.io/nf-core/ubuntu:20.04' }" + + input: + tuple val(meta), path(archive) + + output: + tuple val(meta), path("$gunzip"), emit: gunzip + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + gunzip = archive.toString() - '.gz' + """ + # Not calling gunzip itself because it creates files + # with the original group ownership rather than the + # default one for that user / the work directory + gzip \\ + -cd \\ + $args \\ + $archive \\ + > $gunzip + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//') + END_VERSIONS + """ + + stub: + gunzip = archive.toString() - '.gz' + """ + touch $gunzip + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/gunzip/meta.yml b/modules/nf-core/gunzip/meta.yml new file mode 100644 index 00000000..231034f2 --- /dev/null +++ b/modules/nf-core/gunzip/meta.yml @@ -0,0 +1,39 @@ +name: gunzip +description: Compresses and decompresses files. +keywords: + - gunzip + - compression + - decompression +tools: + - gunzip: + description: | + gzip is a file format and a software application used for file compression and decompression. + documentation: https://www.gnu.org/software/gzip/manual/gzip.html + licence: ["GPL-3.0-or-later"] +input: + - meta: + type: map + description: | + Optional groovy Map containing meta information + e.g. [ id:'test', single_end:false ] + - archive: + type: file + description: File to be compressed/uncompressed + pattern: "*.*" +output: + - gunzip: + type: file + description: Compressed/uncompressed file + pattern: "*.*" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@joseespinosa" + - "@drpatelh" + - "@jfy133" +maintainers: + - "@joseespinosa" + - "@drpatelh" + - "@jfy133" diff --git a/modules/nf-core/gunzip/tests/main.nf.test b/modules/nf-core/gunzip/tests/main.nf.test new file mode 100644 index 00000000..3e9b88b4 --- /dev/null +++ b/modules/nf-core/gunzip/tests/main.nf.test @@ -0,0 +1,36 @@ +nextflow_process { + + name "Test Process GUNZIP" + script "../main.nf" + process "GUNZIP" + tag "gunzip" + tag "modules_nfcore" + tag "modules" + + test("Should run without failures") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([ + [], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ] + ) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} \ No newline at end of file diff --git a/modules/nf-core/gunzip/tests/main.nf.test.snap b/modules/nf-core/gunzip/tests/main.nf.test.snap new file mode 100644 index 00000000..720fd9ff --- /dev/null +++ b/modules/nf-core/gunzip/tests/main.nf.test.snap @@ -0,0 +1,31 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + [ + [ + + ], + "test_1.fastq:md5,4161df271f9bfcd25d5845a1e220dbec" + ] + ], + "1": [ + "versions.yml:md5,54376d32aca20e937a4ec26dac228e84" + ], + "gunzip": [ + [ + [ + + ], + "test_1.fastq:md5,4161df271f9bfcd25d5845a1e220dbec" + ] + ], + "versions": [ + "versions.yml:md5,54376d32aca20e937a4ec26dac228e84" + ] + } + ], + "timestamp": "2023-10-17T15:35:37.690477896" + } +} \ No newline at end of file diff --git a/modules/nf-core/gunzip/tests/tags.yml b/modules/nf-core/gunzip/tests/tags.yml new file mode 100644 index 00000000..fd3f6915 --- /dev/null +++ b/modules/nf-core/gunzip/tests/tags.yml @@ -0,0 +1,2 @@ +gunzip: + - modules/nf-core/gunzip/** diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/environment.yml b/modules/pfr/custom/checkgff3fastacorrespondence/environment.yml new file mode 100644 index 00000000..ec0e86d1 --- /dev/null +++ b/modules/pfr/custom/checkgff3fastacorrespondence/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "custom_checkgff3fastacorrespondence" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::samtools=1.18" diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/main.nf b/modules/pfr/custom/checkgff3fastacorrespondence/main.nf new file mode 100644 index 00000000..09b472c0 --- /dev/null +++ b/modules/pfr/custom/checkgff3fastacorrespondence/main.nf @@ -0,0 +1,25 @@ +process CUSTOM_CHECKGFF3FASTACORRESPONDENCE { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1': + 'quay.io/biocontainers/samtools:1.18--h50ea8bc_1' }" + + input: + tuple val(meta), path(gff3) + path(fasta) + + output: + tuple val(meta), path('*.success.log') , emit: success_log , optional: true + tuple val(meta), path('*.error.log') , emit: error_log , optional: true + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + shell: + prefix = task.ext.prefix ?: "${meta.id}" + template 'check_gff3_fasta_correspondence.sh' +} diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/meta.yml b/modules/pfr/custom/checkgff3fastacorrespondence/meta.yml new file mode 100644 index 00000000..69bbd053 --- /dev/null +++ b/modules/pfr/custom/checkgff3fastacorrespondence/meta.yml @@ -0,0 +1,56 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "custom_checkgff3fastacorrespondence" +description: "A custom bash script which checks the correspondence of a gff3 file with a fasta file" +keywords: + - genome + - gff3 + - annotation + - validation +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: http://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 + licence: ["MIT"] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - gff3: + type: file + description: Input gff3 file + pattern: "*.{gff,gff3}" + - fasta: + type: file + description: Input fasta file + pattern: "*.{fsa,fa,fasta}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - success_log: + type: file + description: Log file for successful validation + pattern: "*.success.log" + - error_log: + type: file + description: Log file for failed validation + pattern: "*.error.log" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/templates/check_gff3_fasta_correspondence.sh b/modules/pfr/custom/checkgff3fastacorrespondence/templates/check_gff3_fasta_correspondence.sh new file mode 100755 index 00000000..611c64b3 --- /dev/null +++ b/modules/pfr/custom/checkgff3fastacorrespondence/templates/check_gff3_fasta_correspondence.sh @@ -0,0 +1,70 @@ +#!/usr/bin/env bash + +# Bump VERSION on edit +VERSION="v1" + +gff3_file="!{gff3}" +fasta_file="!{fasta}" +out_prefix="!{prefix}" +task_process="!{task.process}" + +# Record versions +cat <<-END_VERSIONS > versions.yml +"${task_process}": + samtools: $(echo $(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*$//' ) +END_VERSIONS + +# Requires +# samtools faidx + +## STEP 1 +# Check that gff3 has no identifers that are not in fasta (fasta can +# have ids that are not in gff3 since not all assembly units have gff3 records + +# Extract identifiers from the GFF3 file +gff3_identifiers=$(grep -v '^#' "$gff3_file" | awk '{print $1}' | sort -u) + +# Extract identifiers from the FASTA file +fasta_identifiers=$(grep '^>' "$fasta_file" | awk '{print substr($1, 2)}' | sort -u) + +# Compare identifiers and find any that are present in the GFF3 but not in the FASTA +missing_identifiers=$(comm -23 <(echo "$gff3_identifiers") <(echo "$fasta_identifiers")) + +# Check if any missing identifiers were found +if [[ -n "$missing_identifiers" ]]; then + touch "${out_prefix}.error.log" + echo "Failed to validate gff3 file for: $tag_label" >> "${out_prefix}.error.log" + echo "Fasta file: $fasta_file" >> "${out_prefix}.error.log" + echo "Gff3 file: $gff3_file" >> "${out_prefix}.error.log" + echo "GFF3 file contains identifiers not present in FASTA:" >> "${out_prefix}.error.log" + echo "$missing_identifiers" >> "${out_prefix}.error.log" + exit 0 +fi + +## STEP 2 +# check that there are no coordiantes in gff3 for any seqid that are +# greater than the seq length of the paretn fasta entry + +# Compute sequence lengths using samtools faidx +samtools faidx "$fasta_file" | cut -f 1,2 > sequence_lengths.txt + +# Check GFF3 file for coordinates exceeding sequence lengths +while IFS=$'\t' read -r seqname source feature start end score strand frame attributes && \ + read -r seq seq_length <&3; do + if [[ $start -gt $seq_length || $end -gt $seq_length ]]; then + touch "${out_prefix}.error.log" + echo "Failed to validate gff3 file for: $tag_label" >> "${out_prefix}.error.log" + echo "Fasta file: $fasta_file" >> "${out_prefix}.error.log" + echo "Gff3 file: $gff3_file" >> "${out_prefix}.error.log" + echo "Coordinates exceed sequence length in GFF3 file:" >> "${out_prefix}.error.log" + echo "Sequence: $seqname" >> "${out_prefix}.error.log" + echo "Sequence length: $seq_length" >> "${out_prefix}.error.log" + echo "Start: $start" >> "${out_prefix}.error.log" + echo "End: $end" >> "${out_prefix}.error.log" + exit 0 + fi +done < "$gff3_file" 3< "sequence_lengths.txt" + +touch "${out_prefix}.success.log" +echo "All tests passed..." >> "${out_prefix}.success.log" +exit 0 diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test b/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test new file mode 100644 index 00000000..a45992c2 --- /dev/null +++ b/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test @@ -0,0 +1,70 @@ +nextflow_process { + + name "Test Process CUSTOM_CHECKGFF3FASTACORRESPONDENCE" + script "../main.nf" + process "CUSTOM_CHECKGFF3FASTACORRESPONDENCE" + + tag "modules" + tag "modules_nfcore" + tag "custom" + tag "custom/checkgff3fastacorrespondence" + + test("sarscov2-fasta-gff3-success") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) + ] + + input[1] = [ + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.error_log == [] }, + { assert process.out.success_log != null }, + { assert path(process.out.success_log.get(0).get(1)).getText().contains("All tests passed...")}, + ) + } + + } + + test("sarscov2-gff3-homo_sapiens-fasta-error") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) + ] + + input[1] = [ + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.success_log == [] }, + { assert process.out.error_log != null }, + { assert path(process.out.error_log.get(0).get(1)).getText().contains("GFF3 file contains identifiers not present in FASTA")}, + ) + } + + } + +} \ No newline at end of file diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test.snap b/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test.snap new file mode 100644 index 00000000..261e0dc3 --- /dev/null +++ b/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test.snap @@ -0,0 +1,72 @@ +{ + "sarscov2-gff3-homo_sapiens-fasta-error": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "test" + }, + "test.error.log:md5,8a119170625dc95fb2faa6843fad2c3f" + ] + ], + "2": [ + "versions.yml:md5,c8e0bb60f7422aa6c15db35013620802" + ], + "error_log": [ + [ + { + "id": "test" + }, + "test.error.log:md5,8a119170625dc95fb2faa6843fad2c3f" + ] + ], + "success_log": [ + + ], + "versions": [ + "versions.yml:md5,c8e0bb60f7422aa6c15db35013620802" + ] + } + ], + "timestamp": "2023-11-29T12:24:08.677505" + }, + "sarscov2-fasta-gff3-success": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.success.log:md5,5cad27984e6af4889f7dcf12264fe47b" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,c8e0bb60f7422aa6c15db35013620802" + ], + "error_log": [ + + ], + "success_log": [ + [ + { + "id": "test" + }, + "test.success.log:md5,5cad27984e6af4889f7dcf12264fe47b" + ] + ], + "versions": [ + "versions.yml:md5,c8e0bb60f7422aa6c15db35013620802" + ] + } + ], + "timestamp": "2023-11-29T12:24:04.530428" + } +} \ No newline at end of file diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/tests/tags.yml b/modules/pfr/custom/checkgff3fastacorrespondence/tests/tags.yml new file mode 100644 index 00000000..708130d8 --- /dev/null +++ b/modules/pfr/custom/checkgff3fastacorrespondence/tests/tags.yml @@ -0,0 +1,2 @@ +custom/checkgff3fastacorrespondence: + - "modules/pfr/custom/checkgff3fastacorrespondence/**" diff --git a/modules/pfr/gt/gff3/environment.yml b/modules/pfr/gt/gff3/environment.yml new file mode 100644 index 00000000..8289fb31 --- /dev/null +++ b/modules/pfr/gt/gff3/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "gt_gff3" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::genometools-genometools=1.6.5" diff --git a/modules/pfr/gt/gff3/main.nf b/modules/pfr/gt/gff3/main.nf new file mode 100644 index 00000000..f1774d28 --- /dev/null +++ b/modules/pfr/gt/gff3/main.nf @@ -0,0 +1,51 @@ +process GT_GFF3 { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + 'https://depot.galaxyproject.org/singularity/genometools-genometools:1.6.5--py310h3db02ab_0': + 'quay.io/biocontainers/genometools-genometools:1.6.5--py310h3db02ab_0' }" + + input: + tuple val(meta), path(gff3) + + output: + tuple val(meta), path("*.gt.gff3") , emit: gt_gff3 , optional: true + tuple val(meta), path("*.error.log"), emit: error_log , optional: true + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + gt \\ + gff3 \\ + $args \\ + "$gff3" \\ + > "${prefix}.gt.gff3" \\ + 2> "${prefix}.error.log" \\ + || echo "Errors from gt-gff3 printed to ${prefix}.error.log" + + if grep -q "gt gff3: error:" "${prefix}.error.log"; then + echo "gt-gff3 failed to parse $gff3" + + rm \\ + "${prefix}.gt.gff3" + else + echo "gt-gff3 successfully parsed $gff3" + + mv \\ + "${prefix}.error.log" \\ + gt_gff3.stderr + fi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + genometools: \$(gt --version | head -1 | sed 's/gt (GenomeTools) //') + END_VERSIONS + """ +} diff --git a/modules/pfr/gt/gff3/meta.yml b/modules/pfr/gt/gff3/meta.yml new file mode 100644 index 00000000..5cecd8d0 --- /dev/null +++ b/modules/pfr/gt/gff3/meta.yml @@ -0,0 +1,48 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "gt_gff3" +description: "GenomeTools gt-gff3 utility to parse, possibly transform, and output GFF3 files" +keywords: + - genome + - gff3 + - annotation +tools: + - "gt": + description: "The GenomeTools genome analysis system" + homepage: "https://genometools.org/index.html" + documentation: "https://genometools.org/documentation.html" + tool_dev_url: "https://github.com/genometools/genometools" + doi: "10.1109/TCBB.2013.68" + licence: ["ISC"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - gff3: + type: file + description: Input gff3 file + pattern: "*.{gff,gff3}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - gt_gff3: + type: file + description: Parsed gff3 file produced only if there is no parsing error + pattern: "*.gt.gff3" + - error_log: + type: file + description: Error log if gt-gff3 failed to parse the input gff3 file + pattern: "*.error.log" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@gallvp" +maintainers: + - "@gallvp" diff --git a/modules/pfr/gt/gff3/tests/main.nf.test b/modules/pfr/gt/gff3/tests/main.nf.test new file mode 100644 index 00000000..b57439ef --- /dev/null +++ b/modules/pfr/gt/gff3/tests/main.nf.test @@ -0,0 +1,61 @@ +nextflow_process { + + name "Test Process GT_GFF3" + script "../main.nf" + process "GT_GFF3" + config "./nextflow.config" + + tag "modules" + tag "modules_nfcore" + tag "gt" + tag "gt/gff3" + + test("sarscov2-gff3-valid") { + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.gt_gff3 != null }, + { assert process.out.error_log == [] } + ) + } + + } + + test("sarscov2-gff3-invalid") { + when { + process { + """ + input[0] = Channel.of( + '##gff-version 3', + 'chr22\tID=gene:ENSG00000233995;Name=AP000547.1' + ) + .collectFile(name: 'sample.gff3', newLine: true) + .map { file -> [ [ id:'test' ], file ] } + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.gt_gff3 == [] }, + { assert process.out.error_log != null }, + { assert path(process.out.error_log.get(0).get(1)).getText().contains("gt gff3: error:") } + ) + } + } + +} \ No newline at end of file diff --git a/modules/pfr/gt/gff3/tests/main.nf.test.snap b/modules/pfr/gt/gff3/tests/main.nf.test.snap new file mode 100644 index 00000000..f31e8d1c --- /dev/null +++ b/modules/pfr/gt/gff3/tests/main.nf.test.snap @@ -0,0 +1,72 @@ +{ + "sarscov2-gff3-invalid": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "test" + }, + "test.error.log:md5,31e6117c516f936ec403f792c732bc76" + ] + ], + "2": [ + "versions.yml:md5,9753770dd19a2a306dcf16d4aaf049eb" + ], + "error_log": [ + [ + { + "id": "test" + }, + "test.error.log:md5,31e6117c516f936ec403f792c732bc76" + ] + ], + "gt_gff3": [ + + ], + "versions": [ + "versions.yml:md5,9753770dd19a2a306dcf16d4aaf049eb" + ] + } + ], + "timestamp": "2023-11-28T13:43:34.620429" + }, + "sarscov2-gff3-valid": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.gt.gff3:md5,2ae900237ace415557b8735fac088b85" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,9753770dd19a2a306dcf16d4aaf049eb" + ], + "error_log": [ + + ], + "gt_gff3": [ + [ + { + "id": "test" + }, + "test.gt.gff3:md5,2ae900237ace415557b8735fac088b85" + ] + ], + "versions": [ + "versions.yml:md5,9753770dd19a2a306dcf16d4aaf049eb" + ] + } + ], + "timestamp": "2023-11-28T13:43:31.065832" + } +} \ No newline at end of file diff --git a/modules/pfr/gt/gff3/tests/nextflow.config b/modules/pfr/gt/gff3/tests/nextflow.config new file mode 100644 index 00000000..af562267 --- /dev/null +++ b/modules/pfr/gt/gff3/tests/nextflow.config @@ -0,0 +1,3 @@ +process { + ext.args = '-tidy -retainids' +} diff --git a/modules/pfr/gt/gff3/tests/tags.yml b/modules/pfr/gt/gff3/tests/tags.yml new file mode 100644 index 00000000..ae040309 --- /dev/null +++ b/modules/pfr/gt/gff3/tests/tags.yml @@ -0,0 +1,2 @@ +gt/gff3: + - "modules/pfr/gt/gff3/**" diff --git a/modules/pfr/gt/gff3validator/environment.yml b/modules/pfr/gt/gff3validator/environment.yml new file mode 100644 index 00000000..ea57ebe0 --- /dev/null +++ b/modules/pfr/gt/gff3validator/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "gt_gff3validator" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::genometools-genometools=1.6.5" diff --git a/modules/pfr/gt/gff3validator/main.nf b/modules/pfr/gt/gff3validator/main.nf new file mode 100644 index 00000000..92e5dc2a --- /dev/null +++ b/modules/pfr/gt/gff3validator/main.nf @@ -0,0 +1,50 @@ +process GT_GFF3VALIDATOR { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + 'https://depot.galaxyproject.org/singularity/genometools-genometools:1.6.5--py310h3db02ab_0': + 'quay.io/biocontainers/genometools-genometools:1.6.5--py310h3db02ab_0' }" + + input: + tuple val(meta), path(gff3) + + output: + tuple val(meta), path('*.success.log') , emit: success_log , optional: true + tuple val(meta), path('*.error.log') , emit: error_log , optional: true + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + gt \\ + gff3validator \\ + "$gff3" \\ + > "${prefix}.success.log" \\ + 2> "${prefix}.error.log" \\ + || echo "Errors from gt-gff3validator printed to ${prefix}.error.log" + + if grep -q "input is valid GFF3" "${prefix}.success.log"; then + echo "Validation successful..." + + mv \\ + "${prefix}.error.log" \\ + gt_gff3validator.stderr + else + echo "Validation failed..." + + mv \\ + "${prefix}.success.log" \\ + gt_gff3validator.stdout + fi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + genometools: \$(gt --version | head -1 | sed 's/gt (GenomeTools) //') + END_VERSIONS + """ +} diff --git a/modules/pfr/gt/gff3validator/meta.yml b/modules/pfr/gt/gff3validator/meta.yml new file mode 100644 index 00000000..3322faf9 --- /dev/null +++ b/modules/pfr/gt/gff3validator/meta.yml @@ -0,0 +1,49 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "gt_gff3validator" +description: "GenomeTools gt-gff3validator utility to strictly validate a GFF3 file" +keywords: + - genome + - gff3 + - annotation + - validation +tools: + - "gt": + description: "The GenomeTools genome analysis system" + homepage: "https://genometools.org/index.html" + documentation: "https://genometools.org/documentation.html" + tool_dev_url: "https://github.com/genometools/genometools" + doi: "10.1109/TCBB.2013.68" + licence: ["ISC"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - gff3: + type: file + description: Input gff3 file + pattern: "*.{gff,gff3}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - success_log: + type: file + description: Log file for successful validation + pattern: "*.success.log" + - error_log: + type: file + description: Log file for failed validation + pattern: "*.error.log" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/pfr/gt/gff3validator/tests/main.nf.test b/modules/pfr/gt/gff3validator/tests/main.nf.test new file mode 100644 index 00000000..9addd7d8 --- /dev/null +++ b/modules/pfr/gt/gff3validator/tests/main.nf.test @@ -0,0 +1,63 @@ +nextflow_process { + + name "Test Process GT_GFF3VALIDATOR" + script "../main.nf" + process "GT_GFF3VALIDATOR" + + tag "modules" + tag "modules_nfcore" + tag "gt" + tag "gt/gff3validator" + + test("custom-gff3-valid") { + + when { + process { + """ + input[0] = Channel.of( + '##gff-version 3', + 'chr22\thavana\tpseudogene\t16572027\t16574637\t.\t+\t.\tID=gene:ENSG00000233995;Name=AP000547.1' + ) + .collectFile(name: 'sample.gff3', newLine: true) + .map { file -> [ [ id:'test' ], file ] } + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.error_log == [] }, + { assert process.out.success_log != null }, + { assert path(process.out.success_log.get(0).get(1)).getText().contains("input is valid GFF3") } + ) + } + + } + + test("sarscov2-gff3-invalid") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_gff3'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.success_log == [] }, + { assert process.out.error_log != null }, + { assert path(process.out.error_log.get(0).get(1)).getText().contains("gt gff3validator: error:") } + ) + } + + } +} \ No newline at end of file diff --git a/modules/pfr/gt/gff3validator/tests/main.nf.test.snap b/modules/pfr/gt/gff3validator/tests/main.nf.test.snap new file mode 100644 index 00000000..0b6f065a --- /dev/null +++ b/modules/pfr/gt/gff3validator/tests/main.nf.test.snap @@ -0,0 +1,72 @@ +{ + "sarscov2-gff3-invalid": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "test" + }, + "test.error.log:md5,c5d16b263a87072a13cca44fd811b8e2" + ] + ], + "2": [ + "versions.yml:md5,5927673eb73a8c22408643d224414215" + ], + "error_log": [ + [ + { + "id": "test" + }, + "test.error.log:md5,c5d16b263a87072a13cca44fd811b8e2" + ] + ], + "success_log": [ + + ], + "versions": [ + "versions.yml:md5,5927673eb73a8c22408643d224414215" + ] + } + ], + "timestamp": "2023-11-29T11:09:23.708792" + }, + "custom-gff3-valid": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.success.log:md5,b11ca5c18c865fc808ea0fef0b07da30" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,5927673eb73a8c22408643d224414215" + ], + "error_log": [ + + ], + "success_log": [ + [ + { + "id": "test" + }, + "test.success.log:md5,b11ca5c18c865fc808ea0fef0b07da30" + ] + ], + "versions": [ + "versions.yml:md5,5927673eb73a8c22408643d224414215" + ] + } + ], + "timestamp": "2023-11-29T11:09:19.530068" + } +} \ No newline at end of file diff --git a/modules/pfr/gt/gff3validator/tests/tags.yml b/modules/pfr/gt/gff3validator/tests/tags.yml new file mode 100644 index 00000000..e247d55e --- /dev/null +++ b/modules/pfr/gt/gff3validator/tests/tags.yml @@ -0,0 +1,2 @@ +gt/gff3validator: + - "modules/pfr/gt/gff3validator/**" diff --git a/modules/pfr/gt/stat/environment.yml b/modules/pfr/gt/stat/environment.yml new file mode 100644 index 00000000..dca959b3 --- /dev/null +++ b/modules/pfr/gt/stat/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "gt_stat" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::genometools-genometools=1.6.5" diff --git a/modules/pfr/gt/stat/main.nf b/modules/pfr/gt/stat/main.nf new file mode 100644 index 00000000..ad8da8a9 --- /dev/null +++ b/modules/pfr/gt/stat/main.nf @@ -0,0 +1,35 @@ +process GT_STAT { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + 'https://depot.galaxyproject.org/singularity/genometools-genometools:1.6.5--py310h3db02ab_0': + 'quay.io/biocontainers/genometools-genometools:1.6.5--py310h3db02ab_0' }" + + input: + tuple val(meta), path(gff3) + + output: + tuple val(meta), path("*.gt.stat.yml") , emit: stats + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + gt \\ + stat \\ + $args \\ + "$gff3" \\ + > "${prefix}.gt.stat.yml" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + genometools: \$(gt --version | head -1 | sed 's/gt (GenomeTools) //') + END_VERSIONS + """ +} diff --git a/modules/pfr/gt/stat/meta.yml b/modules/pfr/gt/stat/meta.yml new file mode 100644 index 00000000..203059a6 --- /dev/null +++ b/modules/pfr/gt/stat/meta.yml @@ -0,0 +1,46 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "gt_stat" +description: "GenomeTools gt-stat utility to show statistics about features contained in GFF3 files" +keywords: + - genome + - gff3 + - annotation + - statistics + - stats +tools: + - "gt": + description: "The GenomeTools genome analysis system" + homepage: "https://genometools.org/index.html" + documentation: "https://genometools.org/documentation.html" + tool_dev_url: "https://github.com/genometools/genometools" + doi: "10.1109/TCBB.2013.68" + licence: ["ISC"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - gff3: + type: file + description: Input gff3 file + pattern: "*.{gff,gff3}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - stats: + type: file + description: Stats file in yaml format + pattern: "*.gt.stat.yml" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/pfr/gt/stat/tests/main.nf.test b/modules/pfr/gt/stat/tests/main.nf.test new file mode 100644 index 00000000..8736d013 --- /dev/null +++ b/modules/pfr/gt/stat/tests/main.nf.test @@ -0,0 +1,37 @@ +nextflow_process { + + name "Test Process GT_STAT" + script "../main.nf" + process "GT_STAT" + + tag "modules" + tag "modules_nfcore" + tag "gt" + tag "gt/stat" + + test("sarscov2-gff3") { + + when { + process { + """ + input[0] = Channel.of( + "##gff-version 3" + + file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true).getText().toLowerCase() + ) + .collectFile(name: 'sample.gff3', newLine: true) + .map { file -> [ [ id:'test' ], file ] } + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert path(process.out.stats.get(0).get(1)).getText().contains("cdss: 12") } + ) + } + + } + +} \ No newline at end of file diff --git a/modules/pfr/gt/stat/tests/main.nf.test.snap b/modules/pfr/gt/stat/tests/main.nf.test.snap new file mode 100644 index 00000000..2fcfb8a8 --- /dev/null +++ b/modules/pfr/gt/stat/tests/main.nf.test.snap @@ -0,0 +1,31 @@ +{ + "sarscov2-gff3": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.gt.stat.yml:md5,ebba7831ddbf916b8bbea675ba8693b5" + ] + ], + "1": [ + "versions.yml:md5,a184b50afb2ad6dd2d3d37b0a211dd71" + ], + "stats": [ + [ + { + "id": "test" + }, + "test.gt.stat.yml:md5,ebba7831ddbf916b8bbea675ba8693b5" + ] + ], + "versions": [ + "versions.yml:md5,a184b50afb2ad6dd2d3d37b0a211dd71" + ] + } + ], + "timestamp": "2023-11-29T11:34:48.057277" + } +} \ No newline at end of file diff --git a/modules/pfr/gt/stat/tests/tags.yml b/modules/pfr/gt/stat/tests/tags.yml new file mode 100644 index 00000000..46be6341 --- /dev/null +++ b/modules/pfr/gt/stat/tests/tags.yml @@ -0,0 +1,2 @@ +gt/stat: + - "modules/pfr/gt/stat/**" diff --git a/nextflow.config b/nextflow.config index c49c9eb5..8ade16d1 100644 --- a/nextflow.config +++ b/nextflow.config @@ -230,6 +230,7 @@ apptainer { manifest { name = "AssemblyQC" nextflowVersion = '!>=22.04.3' + version = '1.4' } includeConfig './conf/modules.config' diff --git a/subworkflows/local/busco.nf b/subworkflows/local/busco.nf index 10519bc5..30c0b006 100644 --- a/subworkflows/local/busco.nf +++ b/subworkflows/local/busco.nf @@ -1,28 +1,23 @@ -nextflow.enable.dsl=2 - workflow BUSCO { take: - tuple_of_hap_file_lineage + tuple_of_hap_file_lineage main: - if (!params.busco.skip) { - RUN_BUSCO(tuple_of_hap_file_lineage) - | collect - | set {ch_busco_summaries} - - CREATE_PLOT(ch_busco_summaries) - .set { ch_busco_plot } - - ch_busco_summaries - .mix(ch_busco_plot) - .collect() - .set { ch_outputs } - } else { - ch_outputs = Channel.of([]) - } + // MODULE: RUN_BUSCO + RUN_BUSCO ( tuple_of_hap_file_lineage ) + + ch_busco_summaries = RUN_BUSCO.out.summary + | collect + + // MODULE: RUN_BUSCO + CREATE_PLOT ( ch_busco_summaries ) + + ch_busco_plot = CREATE_PLOT.out.png emit: - list_of_outputs = ch_outputs + summary = RUN_BUSCO.out.summary + plot = ch_busco_plot + versions = Channel.empty().mix(RUN_BUSCO.out.versions.first()) } process RUN_BUSCO { @@ -33,32 +28,37 @@ process RUN_BUSCO { 'https://depot.galaxyproject.org/singularity/busco:5.2.2--pyhdfd78af_0': 'quay.io/biocontainers/busco:5.2.2--pyhdfd78af_0' }" - publishDir "${params.outdir}/busco", mode: 'copy' - input: - tuple val(hap_name), path(fasta_file), val(lineage_dataset) + tuple val(hap_name), path(fasta_file), val(lineage_dataset) output: - path "${hap_name}/short_summary.specific.${lineage_dataset}.${hap_name}_${lineage_split}.txt" + path "${hap_name}/short_summary.specific.${lineage_dataset}.${hap_name}_${lineage_split}.txt" , emit: summary + path "versions.yml" , emit: versions script: - def lineages_path = params.busco.download_path ? "--download_path ${params.busco.download_path}" : '' - def lineage_to_split = "${lineage_dataset}"; - def parts = lineage_to_split.split("_"); - lineage_split = parts[0]; - - """ - busco \ - -m ${params.busco.mode} \ - -o ${hap_name} \ - -i $fasta_file \ - -l ${lineage_dataset} \ - --update-data \ - $lineages_path \ - -c ${task.cpus} - - mv "${hap_name}/short_summary.specific.${lineage_dataset}.${hap_name}.txt" "${hap_name}/short_summary.specific.${lineage_dataset}.${hap_name}_${lineage_split}.txt" - """ + def lineages_path = params.busco.download_path ? "--download_path ${params.busco.download_path}" : '' + def lineage_to_split = "${lineage_dataset}"; + def parts = lineage_to_split.split("_"); + lineage_split = parts[0]; + + """ + busco \ + -m ${params.busco.mode} \ + -o ${hap_name} \ + -i $fasta_file \ + -l ${lineage_dataset} \ + --update-data \ + $lineages_path \ + -c ${task.cpus} + + mv "${hap_name}/short_summary.specific.${lineage_dataset}.${hap_name}.txt" \\ + "${hap_name}/short_summary.specific.${lineage_dataset}.${hap_name}_${lineage_split}.txt" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + busco: \$( busco --version 2>&1 | sed 's/^BUSCO //' ) + END_VERSIONS + """ } process CREATE_PLOT { @@ -72,13 +72,13 @@ process CREATE_PLOT { publishDir params.outdir, mode: 'copy' input: - path "short_summary.*", stageAs: 'busco/*' + path "short_summary.*", stageAs: 'busco/*' output: - path 'busco/*.png' + path 'busco/*.png', emit: png script: - """ - generate_plot.py -wd ./busco - """ + """ + generate_plot.py -wd ./busco + """ } diff --git a/subworkflows/local/create_hic_file.nf b/subworkflows/local/create_hic_file.nf index 2c5af32d..8ce10049 100644 --- a/subworkflows/local/create_hic_file.nf +++ b/subworkflows/local/create_hic_file.nf @@ -1,5 +1,3 @@ -nextflow.enable.dsl=2 - include { MAKE_AGP_FROM_FASTA } from '../../modules/local/make_agp_from_fasta.nf' include { AGP2_ASSEMBLY } from '../../modules/local/agp2_assembly.nf' include { ASSEMBLY2_BEDPE } from '../../modules/local/assembly2_bedpe.nf' @@ -9,28 +7,21 @@ include { RUN_ASSEMBLY_VISUALIZER } from '../../modules/local/run_assembly_vis workflow CREATE_HIC_FILE { take: - create_hic_file_inputs // [sample_id.on.tag, assembly_fasta, alignment_bam] + tuple_of_tag_fa_bam main: - create_hic_file_inputs - | map { - [it[0], it[1]] // [sample_id.on.tag, assembly_fasta] - } - | MAKE_AGP_FROM_FASTA - | AGP2_ASSEMBLY - | ASSEMBLY2_BEDPE + // MODULE: MAKE_AGP_FROM_FASTA | AGP2_ASSEMBLY | ASSEMBLY2_BEDPE + MAKE_AGP_FROM_FASTA ( tuple_of_tag_fa_bam.map { tag, fa, bam -> [ tag, fa ] } ) + | AGP2_ASSEMBLY + | ASSEMBLY2_BEDPE - create_hic_file_inputs - | map { - [it[0], it[2]] // [sample_id.on.tag, alignment_bam] - } - | MATLOCK_BAM2_JUICER - | JUICER_SORT + // MODULE: MATLOCK_BAM2_JUICER | JUICER_SORT + MATLOCK_BAM2_JUICER ( tuple_of_tag_fa_bam.map { tag, fa, bam -> [ tag, bam ] } ) + | JUICER_SORT - AGP2_ASSEMBLY.out.agp_assembly_file - | join(JUICER_SORT.out.sorted_links_txt_file) // [sample_id.on.tag, agp_assembly_file, sorted_links_txt_file] - | RUN_ASSEMBLY_VISUALIZER + // MODULE: RUN_ASSEMBLY_VISUALIZER + RUN_ASSEMBLY_VISUALIZER ( AGP2_ASSEMBLY.out.assembly.join(JUICER_SORT.out.links) ) emit: - hic_file = RUN_ASSEMBLY_VISUALIZER.out.hic_file // [sample_id_on_tag, hic_file] + hic = RUN_ASSEMBLY_VISUALIZER.out.hic } diff --git a/subworkflows/local/hic_contact_map.nf b/subworkflows/local/hic_contact_map.nf index 7c688f12..e102a981 100644 --- a/subworkflows/local/hic_contact_map.nf +++ b/subworkflows/local/hic_contact_map.nf @@ -1,45 +1,44 @@ -nextflow.enable.dsl=2 - include { FASTQ_BWA_MEM_SAMBLASTER } from '../pfr/fastq_bwa_mem_samblaster/main' include { CREATE_HIC_FILE } from './create_hic_file.nf' - include { HIC_QC } from '../../modules/local/hic_qc.nf' workflow HIC_CONTACT_MAP { take: - reads // [ val(meta), [ fq ] ] - fasta // [ val(tag), fasta ] + reads // [ val(meta), [ fq ] ] + fasta // [ val(tag), fasta ] main: - if (!params.hic.skip) { - - FASTQ_BWA_MEM_SAMBLASTER( - reads, - fasta.map { tag, fasta -> [ [ id: tag ], fasta, [] ] } - ) - .bam - | map { meta, bam -> [ meta.ref_id, meta, bam ] } - | join( - fasta - ) - | map { ref_id, meta, bam, fasta -> - [ "${meta.id}.on.${meta.ref_id}", fasta, bam ] - } - | set { ch_fasta_bam } - - HIC_QC ( ch_fasta_bam.map { id, fasta, bam -> [ id, bam ] } ) - - ch_fasta_bam - | CREATE_HIC_FILE - | HIC2_HTML - | collect - | set { ch_list_of_html_files } - } else { - ch_list_of_html_files = Channel.of([]) - } + + // SUBWORKFLOW: FASTQ_BWA_MEM_SAMBLASTER + FASTQ_BWA_MEM_SAMBLASTER( + reads, + fasta.map { tag, fasta -> [ [ id: tag ], fasta, [] ] } + ) + + ch_bam = FASTQ_BWA_MEM_SAMBLASTER.out.bam + + ch_fasta_and_bam = ch_bam + | map { meta, bam -> [ meta.ref_id, meta, bam ] } + | join( + fasta + ) + | map { ref_id, meta, bam, fasta -> + [ "${meta.id}.on.${meta.ref_id}", fasta, bam ] + } + + // MODULE: HIC_QC + HIC_QC ( ch_fasta_and_bam.map { id, fasta, bam -> [ id, bam ] } ) + + // SUBWORKFLOW: CREATE_HIC_FILE | MODULE: HIC2_HTML + CREATE_HIC_FILE ( ch_fasta_and_bam ) + | HIC2_HTML + + ch_versions = Channel.empty() + | mix(FASTQ_BWA_MEM_SAMBLASTER.out.versions) emit: - list_of_html_files = ch_list_of_html_files + html = HIC2_HTML.out.html + versions = ch_versions } process HIC2_HTML { @@ -50,14 +49,14 @@ process HIC2_HTML { publishDir "${params.outdir}/hic", mode: 'copy' input: - tuple val(sample_id_on_tag), path(hic_file) + tuple val(sample_id_on_tag), path(hic_file) output: - path "*.html" + path "*.html", emit: html script: - """ - file_name="$hic_file" - hic_2_html_fc62f04.py "$hic_file" > "\${file_name%.*}.html" - """ + """ + file_name="$hic_file" + hic_2_html_fc62f04.py "$hic_file" > "\${file_name%.*}.html" + """ } diff --git a/subworkflows/local/kraken2.nf b/subworkflows/local/kraken2.nf index dc79884f..9f054dfd 100644 --- a/subworkflows/local/kraken2.nf +++ b/subworkflows/local/kraken2.nf @@ -1,48 +1,43 @@ -nextflow.enable.dsl=2 - include { UNTAR } from '../../modules/nf-core/untar/main.nf' workflow KRAKEN2 { take: - tuple_of_hap_file - db_path // val + tuple_of_hap_file + db_path // channel: path main: - if (!params.kraken2.skip) { - - ch_tar_db = "$db_path".endsWith('.tar.gz') - ? Channel.of(file(db_path, checkIfExists:true)) - : Channel.empty() - - ch_untar_db = "$db_path".endsWith('.tar.gz') - ? Channel.empty() - : Channel.of(file(db_path, checkIfExists:true)) - - ch_tar_db - | map { tar -> [ [ id: "kraken2_db" ], tar ] } - | UNTAR - - UNTAR.out.untar - | map { meta, untar -> untar } - | mix( - ch_untar_db - ) - | combine(tuple_of_hap_file) - | set { ch_kraken2_inputs } - - RUN_KRAKEN2( - ch_kraken2_inputs.map { db, tag, fasta -> [ tag, fasta ] }, - ch_kraken2_inputs.map { db, tag, fasta -> db } - ) - | KRONA_PLOT - | collect - | set { ch_list_of_kraken2_outputs } - } else { - ch_list_of_kraken2_outputs = Channel.of([]) - } + ch_tar_db = db_path + | filter { db -> "$db".endsWith('.tar.gz') } + + ch_untar_db = db_path + | filter { db -> !( "$db".endsWith('.tar.gz') ) } + + // MODULE: UNTAR + UNTAR ( ch_tar_db.map { tar -> [ [ id: "kraken2_db" ], tar ] } ) + + ch_kraken2_inputs = UNTAR.out.untar + | map { meta, untar -> untar } + | mix( + ch_untar_db + ) + | combine(tuple_of_hap_file) + + // MODULE: RUN_KRAKEN2 + RUN_KRAKEN2( + ch_kraken2_inputs.map { db, tag, fasta -> [ tag, fasta ] }, + ch_kraken2_inputs.map { db, tag, fasta -> db } + ) + + // MODULE: KRONA_PLOT + KRONA_PLOT ( RUN_KRAKEN2.out.report ) + + ch_versions = Channel.empty() + | mix(RUN_KRAKEN2.out.versions.first()) + | mix(UNTAR.out.versions.first()) emit: - list_of_outputs = ch_list_of_kraken2_outputs + plot = KRONA_PLOT.out.plot + versions = ch_versions } process RUN_KRAKEN2 { @@ -54,25 +49,29 @@ process RUN_KRAKEN2 { 'https://depot.galaxyproject.org/singularity/kraken2:2.1.2--pl5321h9f5acd7_2': 'quay.io/biocontainers/kraken2:2.1.2--pl5321h9f5acd7_2' }" - publishDir "${params.outdir}/kraken2", mode: 'copy' - input: - tuple val(hap_name), path(fasta_file) - path db_path + tuple val(hap_name), path(fasta_file) + path db_path output: - tuple val(hap_name), path("*.kraken2.cut"), path("*.kraken2.report") + tuple val(hap_name), path("*.kraken2.cut"), path("*.kraken2.report"), emit: report + path "versions.yml" , emit: versions script: - """ - kraken2 \ - --output "${hap_name}.kraken2.cut" \ - --report "${hap_name}.kraken2.report" \ - --use-names \ - --db $db_path \ - --threads ${task.cpus} \ + """ + kraken2 \\ + --output "${hap_name}.kraken2.cut" \\ + --report "${hap_name}.kraken2.report" \\ + --use-names \\ + --db $db_path \\ + --threads ${task.cpus} \\ $fasta_file > kraken2.log - """ + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + kraken2: \$(echo \$(kraken2 --version 2>&1) | sed 's/^.*Kraken version //; s/ .*\$//') + END_VERSIONS + """ } process KRONA_PLOT { @@ -83,14 +82,14 @@ process KRONA_PLOT { publishDir "${params.outdir}/kraken2", mode: 'copy' input: - tuple val(hap_name), path(kraken2_cut), path(kraken2_report) + tuple val(hap_name), path(kraken2_cut), path(kraken2_report) output: - tuple path("*.kraken2.krona.cut"), path("*.kraken2.krona.html") + tuple path("*.kraken2.krona.cut"), path("*.kraken2.krona.html"), emit: plot script: - """ - perl -lane '@a=split /\\t/; if (\$a[2] =~ /taxid\\s+(\\d+)/) {print "\$a[1]\\t\$1\\t1\\t\$a[3]";}' $kraken2_cut > "${hap_name}.kraken2.krona.cut" - ktImportTaxonomy -i -o "${hap_name}.kraken2.krona.html" -m "4" "${hap_name}.kraken2.krona.cut" - """ + """ + perl -lane '@a=split /\\t/; if (\$a[2] =~ /taxid\\s+(\\d+)/) {print "\$a[1]\\t\$1\\t1\\t\$a[3]";}' $kraken2_cut > "${hap_name}.kraken2.krona.cut" + ktImportTaxonomy -i -o "${hap_name}.kraken2.krona.html" -m "4" "${hap_name}.kraken2.krona.cut" + """ } diff --git a/subworkflows/local/ncbi_fcs_adaptor.nf b/subworkflows/local/ncbi_fcs_adaptor.nf deleted file mode 100644 index 48de75c9..00000000 --- a/subworkflows/local/ncbi_fcs_adaptor.nf +++ /dev/null @@ -1,107 +0,0 @@ -nextflow.enable.dsl=2 - -workflow NCBI_FCS_ADAPTOR { - take: - tuple_of_tag_file - - main: - if (!params.ncbi_fcs_adaptor.skip) { - SCREEN_SAMPLE(tuple_of_tag_file) - | set { ch_report } - - ch_report - .map { - it[1] // report file path - } - .collect() - .set { ch_all_reports } - - ch_report - | CHECK_CONTAMINATION - | map { - def itTokes = "$it".tokenize(':') - def status = itTokes[1] - def tag = itTokes[2] - - def isClean = status == "CLEAN" - - [tag, isClean] - } - | set { ch_tuple_tag_is_clean } // [tag, is_clean flag] - - ch_tuple_tag_is_clean - | map { - def tag = it[0] - def isClean = it[1] - - if (!isClean) { - log.warn(""" - Adaptor contamination detected in ${tag}. - See the report for further details. - """.stripIndent()) - } - } - } else { - tuple_of_tag_file - .map { - [it[0], true] // [tag, true] - } - .set { ch_tuple_tag_is_clean } - - ch_all_reports = Channel.of([]) - } - - emit: - is_clean_status = ch_tuple_tag_is_clean - reports = ch_all_reports -} - -process SCREEN_SAMPLE { - tag "${hap_name}" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://ftp.ncbi.nlm.nih.gov/genomes/TOOLS/FCS/releases/0.4.0/fcs-adaptor.sif': - 'docker.io/ncbi/fcs-adaptor:0.4.0' }" - - publishDir "${params.outdir}/ncbi_fcs_adaptor", mode: 'copy' - - input: - tuple val(hap_name), path(fasta_file) - - output: - tuple val(hap_name), path("${hap_name}_fcs_adaptor_report.tsv") - - script: - """ - mkdir "${hap_name}_outputdir" - - /app/fcs/bin/av_screen_x \ - -o "${hap_name}_outputdir" \ - --${params.ncbi_fcs_adaptor.empire} \ - "${fasta_file}" - - mv "${hap_name}_outputdir/fcs_adaptor_report.txt" "./${hap_name}_fcs_adaptor_report.tsv" - """ -} - -process CHECK_CONTAMINATION { - tag "${hap_name}" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/ubuntu:20.04': - 'quay.io/nf-core/ubuntu:20.04' }" - - input: - tuple val(hap_name), path(report_tsv) - - output: - stdout - - script: - """ - num_lines=\$(cat $report_tsv | wc -l) - [[ \$num_lines -gt 1 ]] && echo -n "CHECK_ADAPTOR_CONTAMINATION:CONTAMINATED:$hap_name" || echo -n "CHECK_ADAPTOR_CONTAMINATION:CLEAN:$hap_name" - """ -} diff --git a/subworkflows/local/ncbi_fcs_gx.nf b/subworkflows/local/ncbi_fcs_gx.nf index 421cdb58..fc3832a1 100644 --- a/subworkflows/local/ncbi_fcs_gx.nf +++ b/subworkflows/local/ncbi_fcs_gx.nf @@ -1,85 +1,43 @@ -nextflow.enable.dsl=2 - workflow NCBI_FCS_GX { take: - tuple_of_tag_file - db_path // val + tuple_of_tag_file + db_path // channel: path main: - if (!params.ncbi_fcs_gx.skip) { - - tuple_of_tag_file - | SETUP_SAMPLE - | collect - | set {ch_all_samples} - - SCREEN_SAMPLES(ch_all_samples, file(db_path, checkIfExists:true)) - - // Clean/contaminated branching - SCREEN_SAMPLES - .out - .fcs_gx_reports - | flatten - | map { - def parts = it.getName().split("\\.") - def tag = parts[0] - [tag, it] - } - | CHECK_CONTAMINATION - | map { - def itTokes = "$it".tokenize(':') - def status = itTokes[1] - def tag = itTokes[2] - - def isClean = status == "CLEAN" - - [tag, isClean] - } - | set { ch_tuple_tag_is_clean } // [tag, is_clean flag] - - ch_tuple_tag_is_clean - | map { - def tag = it[0] - def isClean = it[1] - - if (!isClean) { - log.warn(""" - Foreign organism contamination detected in ${tag}. - See the report for further details. - """.stripIndent()) - } - } - - // Taxonomy Krona plot - SCREEN_SAMPLES - .out - .fcs_gx_taxonomies - | flatten - | map { - def parts = it.getName().split("\\.") - def tag = parts[0] - [tag, it] - } - | FCS_GX_KRONA_PLOT - | flatten - | mix( - SCREEN_SAMPLES.out.fcs_gx_reports.flatten() - ) - | collect - | set { ch_fcs_gx_reports } - } else { - tuple_of_tag_file - .map { - [it[0], true] // [tag, true] - } - .set { ch_tuple_tag_is_clean } - - ch_fcs_gx_reports = Channel.of([]) - } - + // MODULE: SETUP_SAMPLE + SETUP_SAMPLE ( tuple_of_tag_file ) + + ch_all_samples = SETUP_SAMPLE.out.fsata + | collect + + // MODULE: SCREEN_SAMPLES + SCREEN_SAMPLES ( ch_all_samples, db_path ) + + ch_gx_report = SCREEN_SAMPLES.out.fcs_gx_reports + | flatten + | map { + def parts = it.getName().split("\\.") + def tag = parts[0] + [tag, it] + } + + ch_gx_taxonomy = SCREEN_SAMPLES.out.fcs_gx_taxonomies + | flatten + | map { + def parts = it.getName().split("\\.") + def tag = parts[0] + [tag, it] + } + + // MODULE: FCS_GX_KRONA_PLOT + FCS_GX_KRONA_PLOT ( ch_gx_taxonomy ) + + ch_gx_taxonomy_plot = FCS_GX_KRONA_PLOT.out.plot emit: - is_clean_status = ch_tuple_tag_is_clean - fcs_gx_reports = ch_fcs_gx_reports + gx_report = ch_gx_report + gx_taxonomy = ch_gx_taxonomy + gx_taxonomy_plot = ch_gx_taxonomy_plot + versions = Channel.empty().mix(SCREEN_SAMPLES.out.versions.first()) } process SETUP_SAMPLE { @@ -91,15 +49,15 @@ process SETUP_SAMPLE { 'quay.io/nf-core/ubuntu:20.04' }" input: - tuple val(hap_name), path(fasta_file) + tuple val(hap_name), path(fasta_file) output: - path 'fasta.file.for.*.fasta' + path 'fasta.file.for.*.fasta', emit: fsata script: - """ - ln -s $fasta_file "fasta.file.for.${hap_name}.fasta" - """ + """ + ln -s $fasta_file "fasta.file.for.${hap_name}.fasta" + """ } @@ -113,49 +71,32 @@ process SCREEN_SAMPLES { 'https://ftp.ncbi.nlm.nih.gov/genomes/TOOLS/FCS/releases/0.4.0/fcs-gx.sif': 'docker.io/ncbi/fcs-gx:0.4.0' }" - publishDir "${params.outdir}/ncbi_fcs_gx", mode: 'copy' - - input: - path samples - path db_path - - output: - path "*.fcs_gx_report.txt", emit: fcs_gx_reports - path "*.taxonomy.rpt", emit: fcs_gx_taxonomies - - script: - """ - for sample_fasta in $samples; - do - sample_tag=\$(echo "\$sample_fasta" | sed 's/fasta.file.for.//g' | sed 's/.fasta//g') - python3 /app/bin/run_gx --fasta ./\$sample_fasta --out-dir ./ --gx-db $db_path --tax-id "${params.ncbi_fcs_gx.tax_id}" - - mv "\${sample_fasta%.fasta}.${params.ncbi_fcs_gx.tax_id}.fcs_gx_report.txt" "\${sample_tag}.fcs_gx_report.txt" - mv "\${sample_fasta%.fasta}.${params.ncbi_fcs_gx.tax_id}.taxonomy.rpt" "\${sample_tag}.taxonomy.rpt" - done - """ -} - -process CHECK_CONTAMINATION { - tag "${hap_name}" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/ubuntu:20.04': - 'quay.io/nf-core/ubuntu:20.04' }" - input: - tuple val(hap_name), path(report_file) + path samples + path db_path output: - stdout + path "*.fcs_gx_report.txt" , emit: fcs_gx_reports + path "*.taxonomy.rpt" , emit: fcs_gx_taxonomies + path "versions.yml" , emit: versions script: - """ - hap_name=\$(echo "$report_file" | sed 's/.fcs_gx_report.txt//g') - num_lines=\$(cat $report_file | wc -l) - [[ \$num_lines -gt 2 ]] && echo -n "CHECK_GX_CONTAMINATION:CONTAMINATED:\$hap_name" || echo -n "CHECK_GX_CONTAMINATION:CLEAN:\$hap_name" - """ + def VERSION = 0.4 + """ + for sample_fasta in $samples; + do + sample_tag=\$(echo "\$sample_fasta" | sed 's/fasta.file.for.//g' | sed 's/.fasta//g') + python3 /app/bin/run_gx --fasta ./\$sample_fasta --out-dir ./ --gx-db $db_path --tax-id "${params.ncbi_fcs_gx.tax_id}" + + mv "\${sample_fasta%.fasta}.${params.ncbi_fcs_gx.tax_id}.fcs_gx_report.txt" "\${sample_tag}.fcs_gx_report.txt" + mv "\${sample_fasta%.fasta}.${params.ncbi_fcs_gx.tax_id}.taxonomy.rpt" "\${sample_tag}.taxonomy.rpt" + done + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fcs_gx: $VERSION + END_VERSIONS + """ } process FCS_GX_KRONA_PLOT { @@ -166,25 +107,25 @@ process FCS_GX_KRONA_PLOT { publishDir "${params.outdir}/ncbi_fcs_gx", mode: 'copy' input: - tuple val(tag_name), path(fcs_gx_taxonomy) + tuple val(tag_name), path(fcs_gx_taxonomy) output: - tuple path("${tag_name}.inter.tax.rpt.tsv"), path("${tag_name}.fcs.gx.krona.cut"), path("${tag_name}.fcs.gx.krona.html") + tuple path("${tag_name}.inter.tax.rpt.tsv"), path("${tag_name}.fcs.gx.krona.cut"), path("${tag_name}.fcs.gx.krona.html"), emit: plot script: - """ - cat $fcs_gx_taxonomy \ - | awk 'NR>1 {print \$1,\$2,\$6,\$7,\$11,\$32}' FS="\\t" OFS="\\t" \ + """ + cat $fcs_gx_taxonomy \\ + | awk 'NR>1 {print \$1,\$2,\$6,\$7,\$11,\$32}' FS="\\t" OFS="\\t" \\ > "${tag_name}.inter.tax.rpt.tsv" - cat "${tag_name}.inter.tax.rpt.tsv" \ - | awk '\$6 !~ /(bogus|repeat|low-coverage|inconclusive)/ {print \$1,\$4,\$5,\$2}' FS="\\t" OFS="\\t" \ + cat "${tag_name}.inter.tax.rpt.tsv" \\ + | awk '\$6 !~ /(bogus|repeat|low-coverage|inconclusive)/ {print \$1,\$4,\$5,\$2}' FS="\\t" OFS="\\t" \\ > "${tag_name}.fcs.gx.krona.cut" - cat "${tag_name}.inter.tax.rpt.tsv" \ - | awk 'NR>1 && \$6 ~ /(bogus|repeat|low-coverage|inconclusive)/ {print \$1,"0",\$5,\$2}' FS="\\t" OFS="\\t" \ + cat "${tag_name}.inter.tax.rpt.tsv" \\ + | awk 'NR>1 && \$6 ~ /(bogus|repeat|low-coverage|inconclusive)/ {print \$1,"0",\$5,\$2}' FS="\\t" OFS="\\t" \\ >> "${tag_name}.fcs.gx.krona.cut" - ktImportTaxonomy -i -o "${tag_name}.fcs.gx.krona.html" -m "4" "${tag_name}.fcs.gx.krona.cut" - """ + ktImportTaxonomy -i -o "${tag_name}.fcs.gx.krona.html" -m "4" "${tag_name}.fcs.gx.krona.cut" + """ } diff --git a/subworkflows/local/synteny.nf b/subworkflows/local/synteny.nf index ce4742fe..f4fa5b59 100644 --- a/subworkflows/local/synteny.nf +++ b/subworkflows/local/synteny.nf @@ -1,115 +1,128 @@ -nextflow.enable.dsl=2 - -include { GZIP_FASTA } from '../../modules/local/gzip_fasta' +include { GUNZIP as GUNZIP_FASTA } from '../../modules/nf-core/gunzip/main' workflow SYNTENY { take: - tuple_of_tag_fasta_seq_list - tuple_of_tag_xref_fasta_seq_list + tuple_of_tag_fasta_seq_list + tuple_of_tag_xref_fasta_seq_list main: - if(!params.synteny.skip) { - - if(params.synteny.between_target_asm) { - tuple_of_tag_fasta_seq_list - | map { - [it] - } - | collect - | map { - getUniqueWithinCombinations(it) - } - | flatten - | buffer(size:6) - | set { ch_between_target_asm_combinations } - } else { - ch_between_target_asm_combinations = Channel.empty() - } - - tuple_of_tag_xref_fasta_seq_list - | map { - [it[0], it[1]] // [tag, xref fasta file path] - } - | GZIP_FASTA - | join( - tuple_of_tag_xref_fasta_seq_list - ) - | map { - [it[0], it[1], it[3]] // [tag, uncompressed xref fasta file path, seq list] - } - | set { ch_tuple_tag_xref_uncompressed_fasta_seq_list } - - ch_between_target_asm_combinations - .mix( - tuple_of_tag_fasta_seq_list - | combine( - ch_tuple_tag_xref_uncompressed_fasta_seq_list - ) - ) - .tap { ch_full_tap_from_all_combinations } - .map { - ["${it[0]}.on.${it[3]}", it[2], it[5]] // [target.on.reference, target_seq_list, ref_seq_list] - } - .set { ch_seq_lists } - - - ch_full_tap_from_all_combinations - | FILTER_SORT_FASTA_AND_VALIDATE_SEQ_LISTS - | (MUMMER & GET_FASTA_LEN) - - - FILTER_SORT_FASTA_AND_VALIDATE_SEQ_LISTS.out.tags_fasta_files - .map { target, reference, target_fasta, ref_fasta -> - [ "${target}.on.${reference}", target_fasta, ref_fasta ] - } - .join( - MUMMER.out.tag_delta_file - ) - | DNADIFF - | CIRCOS_BUNDLE_LINKS - | ADD_COLOUR_TO_BUNDLE_LINKS - | join(ch_seq_lists) - | RELABEL_BUNDLE_LINKS - | SPLIT_BUNDLE_FILE_BY_TARGET_SEQS - | map { - flattenSplitBundles(it) - } - | flatten - | buffer(size:3) - | set { ch_circos_split_bundle_links } - - GET_FASTA_LEN - .out - .tag_len_files - | join(ch_seq_lists) - | RELABEL_FASTA_LEN - | cross( - ch_circos_split_bundle_links - ) - | map { - [it[0][0], it[1][1], it[1][2], it[0][1], it[0][2]] // [target.on.reference, seq_tag, split_bundle_file, target_seq_len, ref_seq_len] - } - | GENERATE_KARYOTYPE - | join( - ch_circos_split_bundle_links - | map { - ["${it[0]}.${it[1]}", it[2]] // [target.on.reference.seq_tag, split_bundle_file] - } - ) - | CIRCOS - - CIRCOS - .out - .png_file - | collect - | set{ ch_list_of_circos_plots } - } - else { - ch_list_of_circos_plots = Channel.of([]) - } + ch_between_target_asm_combinations = !params.synteny.between_target_asm + ? Channel.empty() + : tuple_of_tag_fasta_seq_list + | map { [it] } + | collect + | map { getUniqueWithinCombinations(it) } + | flatten + | buffer(size:6) + + ch_xref_fa_branch = tuple_of_tag_xref_fasta_seq_list + | map { tag, fa, seq_list -> + [ [ id: tag ], fa ] + } + | branch { meta, fa -> + gz: "$fa".endsWith(".gz") + rest: !"$fa".endsWith(".gz") + } + + // MODULE: GUNZIP_FASTA + GUNZIP_FASTA ( ch_xref_fa_branch.gz ) + + ch_xref_ungz_fasta_seq_list = GUNZIP_FASTA.out.gunzip + | mix( + ch_xref_fa_branch.rest + ) + | map { meta, fa -> [ meta.id, fa ] } + | join( + tuple_of_tag_xref_fasta_seq_list + ) + | map { tag, fa, input_fa, seq_list -> + [ tag, fa, seq_list ] + } + + // MODULE: FILTER_SORT_FASTA_AND_VALIDATE_SEQ_LISTS | MUMMER & GET_FASTA_LEN + ch_seq_lists = ch_between_target_asm_combinations + .mix( + tuple_of_tag_fasta_seq_list + | combine( + ch_xref_ungz_fasta_seq_list + ) + ) + .tap { ch_full_tap_from_all_combinations } + | map { target_tag, target_fa, target_sl, xref_tag, xref_fa, xref_sl -> + [ "${target_tag}.on.${xref_tag}", target_sl, xref_sl ] + } + + FILTER_SORT_FASTA_AND_VALIDATE_SEQ_LISTS ( ch_full_tap_from_all_combinations ) + | ( MUMMER & GET_FASTA_LEN ) + + + ch_dnadiff_inputs = FILTER_SORT_FASTA_AND_VALIDATE_SEQ_LISTS.out.tags_fasta_files + | map { target, reference, target_fasta, ref_fasta -> + [ "${target}.on.${reference}", target_fasta, ref_fasta ] + } + | join( + MUMMER.out.tag_delta_file + ) + + // MODULE: DNADIFF | CIRCOS_BUNDLE_LINKS | ADD_COLOUR_TO_BUNDLE_LINKS + DNADIFF ( ch_dnadiff_inputs ) + | CIRCOS_BUNDLE_LINKS + | ADD_COLOUR_TO_BUNDLE_LINKS + + ch_coloured_links = ADD_COLOUR_TO_BUNDLE_LINKS.out.coloured_bundle_links + + ch_relabel_links_inputs = ch_coloured_links + | join(ch_seq_lists) + + // MODULE: RELABEL_BUNDLE_LINKS | SPLIT_BUNDLE_FILE_BY_TARGET_SEQS + RELABEL_BUNDLE_LINKS ( ch_relabel_links_inputs ) + | SPLIT_BUNDLE_FILE_BY_TARGET_SEQS + + ch_circos_split_bundle_links = SPLIT_BUNDLE_FILE_BY_TARGET_SEQS.out.split_file + | map { flattenSplitBundles(it) } + | flatten + | buffer(size:3) + + // MODULE: RELABEL_FASTA_LEN + ch_relabel_fasta_len_inputs = GET_FASTA_LEN.out.tag_len_files + | join(ch_seq_lists) + + RELABEL_FASTA_LEN ( ch_relabel_fasta_len_inputs ) + + // MODULE: GENERATE_KARYOTYPE + ch_generate_karyotype_inputs = RELABEL_FASTA_LEN.out.relabeled_seq_lengths + | cross( + ch_circos_split_bundle_links + ) + | map { seq_len_tuple, split_bundle_tuple -> + + def target_on_xref = seq_len_tuple[0] + def seq_tag = split_bundle_tuple[1] + def split_bundle_file = split_bundle_tuple[2] + def target_seq_len = seq_len_tuple[1] + def ref_seq_len = seq_len_tuple[2] + + [ target_on_xref, seq_tag, split_bundle_file, target_seq_len, ref_seq_len ] + } + GENERATE_KARYOTYPE ( ch_generate_karyotype_inputs ) + + // MODULE: CIRCOS + ch_circos_inputs = GENERATE_KARYOTYPE.out.karyotype + | join( + ch_circos_split_bundle_links + | map { target_on_xref, seq_tag, txt -> + [ "${target_on_xref}.${seq_tag}", txt ] + } + ) + CIRCOS ( ch_circos_inputs ) + + ch_versions = Channel.empty() + | mix(MUMMER.out.versions.first()) + | mix(CIRCOS.out.versions.first()) emit: - list_of_circos_plots = ch_list_of_circos_plots + plot = CIRCOS.out.png_file + versions = ch_versions } def getUniqueWithinCombinations(inputArray) { @@ -181,17 +194,17 @@ process FILTER_SORT_FASTA_AND_VALIDATE_SEQ_LISTS { 'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }" input: - tuple val(target), path(target_fasta), path(target_seq_list), val(reference), path(ref_fasta), path(ref_seq_list) + tuple val(target), path(target_fasta), path(target_seq_list), val(reference), path(ref_fasta), path(ref_seq_list) output: - tuple val(target), val(reference), path("filtered.ordered.target.fasta"), path("filtered.ordered.ref.fasta"), emit: tags_fasta_files + tuple val(target), val(reference), path("filtered.ordered.target.fasta"), path("filtered.ordered.ref.fasta"), emit: tags_fasta_files script: - """ - validate_seq_lists_1d50376.sh "$target_seq_list" "$ref_seq_list" - samtools faidx $target_fasta \$(awk '{print \$1}' $target_seq_list) > filtered.ordered.target.fasta - samtools faidx $ref_fasta \$(awk '{print \$1}' $ref_seq_list) > filtered.ordered.ref.fasta - """ + """ + validate_seq_lists_1d50376.sh "$target_seq_list" "$ref_seq_list" + samtools faidx $target_fasta \$(awk '{print \$1}' $target_seq_list) > filtered.ordered.target.fasta + samtools faidx $ref_fasta \$(awk '{print \$1}' $ref_seq_list) > filtered.ordered.ref.fasta + """ } process GET_FASTA_LEN { @@ -203,19 +216,19 @@ process GET_FASTA_LEN { 'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }" input: - tuple val(target), val(reference), path(filtered_ordered_target_fasta), path(filtered_ordered_ref_fasta) + tuple val(target), val(reference), path(filtered_ordered_target_fasta), path(filtered_ordered_ref_fasta) output: - tuple val("${target}.on.${reference}"), path("target.seq.lengths"), path("ref.seq.lengths"), emit: tag_len_files + tuple val("${target}.on.${reference}"), path("target.seq.lengths"), path("ref.seq.lengths"), emit: tag_len_files script: - """ - samtools faidx $filtered_ordered_target_fasta - samtools faidx $filtered_ordered_ref_fasta + """ + samtools faidx $filtered_ordered_target_fasta + samtools faidx $filtered_ordered_ref_fasta - cat "${filtered_ordered_target_fasta}.fai" | awk '{print \$1, \$2}' OFS="\\t" > target.seq.lengths - cat "${filtered_ordered_ref_fasta}.fai" | awk '{print \$1, \$2}' OFS="\\t" > ref.seq.lengths - """ + cat "${filtered_ordered_target_fasta}.fai" | awk '{print \$1, \$2}' OFS="\\t" > target.seq.lengths + cat "${filtered_ordered_ref_fasta}.fai" | awk '{print \$1, \$2}' OFS="\\t" > ref.seq.lengths + """ } process MUMMER { @@ -225,20 +238,26 @@ process MUMMER { container "docker.io/staphb/mummer:4.0.0" input: - tuple val(target), val(reference), path(target_fasta), path(ref_fasta) + tuple val(target), val(reference), path(target_fasta), path(ref_fasta) output: - tuple val("${target}.on.${reference}"), path("*.delta"), emit: tag_delta_file + tuple val("${target}.on.${reference}"), path("*.delta"), emit: tag_delta_file + path "versions.yml", emit: versions script: - """ - nucmer \ - --mum \ - -t ${task.cpus} \ - -p "${target}.on.${reference}" \ - $ref_fasta \ + """ + nucmer \ + --mum \\ + -t ${task.cpus} \\ + -p "${target}.on.${reference}" \\ + $ref_fasta \\ $target_fasta - """ + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + nucmer: \$(nucmer -V) + END_VERSIONS + """ } process DNADIFF { @@ -249,28 +268,28 @@ process DNADIFF { container "docker.io/staphb/mummer:4.0.0" input: - tuple val(target_on_ref), path(target_fasta), path(ref_fasta), path(dnadiff_file) + tuple val(target_on_ref), path(target_fasta), path(ref_fasta), path(dnadiff_file) output: - tuple val(target_on_ref), path("*.xcoords"), path("*.report") + tuple val(target_on_ref), path("*.xcoords"), path("*.report") script: - def inter_extension = params.synteny.many_to_many_align == 1 ? 'mcoords' : '1coords' - def out_extension = params.synteny.many_to_many_align == 1 ? 'm.xcoords' : '1.xcoords' - """ - cat \\ - $dnadiff_file \\ - | sed '1s/.*/${ref_fasta} ${target_fasta}/' \\ - > ${target_on_ref}.sed.delta - - dnadiff \\ - -p $target_on_ref \\ - -d ${target_on_ref}.sed.delta - - cat \\ - "${target_on_ref}.${inter_extension}" \\ - > "${target_on_ref}.${out_extension}" - """ + def inter_extension = params.synteny.many_to_many_align == 1 ? 'mcoords' : '1coords' + def out_extension = params.synteny.many_to_many_align == 1 ? 'm.xcoords' : '1.xcoords' + """ + cat \\ + $dnadiff_file \\ + | sed '1s/.*/${ref_fasta} ${target_fasta}/' \\ + > ${target_on_ref}.sed.delta + + dnadiff \\ + -p $target_on_ref \\ + -d ${target_on_ref}.sed.delta + + cat \\ + "${target_on_ref}.${inter_extension}" \\ + > "${target_on_ref}.${out_extension}" + """ } process CIRCOS_BUNDLE_LINKS { @@ -280,22 +299,22 @@ process CIRCOS_BUNDLE_LINKS { container "docker.io/gallvp/circos-tools:v0.23-1_ps" input: - tuple val(target_on_ref), path(coords_file), path(report_file) + tuple val(target_on_ref), path(coords_file), path(report_file) output: - tuple val(target_on_ref), path("*.xcoords.bundle.txt") + tuple val(target_on_ref), path("*.xcoords.bundle.txt") script: - """ - cat $coords_file | awk '{print \$12,\$1,\$2,\$13,\$3,\$4}' OFS="\\t" > "\$(basename $coords_file).links.txt" - - /usr/share/circos/tools/bundlelinks/bin/bundlelinks \ - -links "\$(basename $coords_file).links.txt" \ - -max_gap "${params.synteny.max_gap}" \ - -min_bundle_size "${params.synteny.min_bundle_size}" \ - 1>"\$(basename $coords_file).bundle.txt" \ + """ + cat $coords_file | awk '{print \$12,\$1,\$2,\$13,\$3,\$4}' OFS="\\t" > "\$(basename $coords_file).links.txt" + + /usr/share/circos/tools/bundlelinks/bin/bundlelinks \\ + -links "\$(basename $coords_file).links.txt" \\ + -max_gap "${params.synteny.max_gap}" \\ + -min_bundle_size "${params.synteny.min_bundle_size}" \\ + 1>"\$(basename $coords_file).bundle.txt" \\ 2>bundlelinks.err - """ + """ } process ADD_COLOUR_TO_BUNDLE_LINKS { @@ -305,23 +324,23 @@ process ADD_COLOUR_TO_BUNDLE_LINKS { container "docker.io/gallvp/python3npkgs:v0.4" input: - tuple val(target_on_ref), path(bundle_links) + tuple val(target_on_ref), path(bundle_links) output: - tuple val(target_on_ref), path("*.xcoords.bundle.coloured.txt"), emit: coloured_bundle_links + tuple val(target_on_ref), path("*.xcoords.bundle.coloured.txt"), emit: coloured_bundle_links script: - """ - if [[ "${params.synteny.color_by_contig}" = "1" ]];then - color_circos_bundles_by_contig_943e0fb.py \ - "${bundle_links}" \ + """ + if [[ "${params.synteny.color_by_contig}" = "1" ]];then + color_circos_bundles_by_contig_943e0fb.py \\ + "${bundle_links}" \\ > "\$(basename $bundle_links .bundle.txt).bundle.coloured.txt" - else - add_color_2_circos_bundle_file_943e0fb.pl \ - -i="${bundle_links}" \ + else + add_color_2_circos_bundle_file_943e0fb.pl \\ + -i="${bundle_links}" \\ -o="\$(basename $bundle_links .bundle.txt).bundle.coloured.txt" - fi - """ + fi + """ } process RELABEL_BUNDLE_LINKS { @@ -331,39 +350,39 @@ process RELABEL_BUNDLE_LINKS { container "docker.io/gallvp/python3npkgs:v0.4" input: - tuple val(target_on_ref), path(coloured_bundle_links), path(target_seq_list), path(ref_seq_list) + tuple val(target_on_ref), path(coloured_bundle_links), path(target_seq_list), path(ref_seq_list) output: - tuple val(target_on_ref), path("*.xcoords.bundle.coloured.relabeled.txt"), emit: relabeled_coloured_bundle_links + tuple val(target_on_ref), path("*.xcoords.bundle.coloured.relabeled.txt"), emit: relabeled_coloured_bundle_links script: - """ - #!/usr/bin/env python + """ + #!/usr/bin/env python - import pandas as pd - import sys - import os + import pandas as pd + import sys + import os - output_file_name = ".".join("$coloured_bundle_links".split(".")[0:-1]) + ".relabeled.txt" + output_file_name = ".".join("$coloured_bundle_links".split(".")[0:-1]) + ".relabeled.txt" - subs_target_seq = pd.read_csv('$target_seq_list', sep='\\t', header=None) - subs_target_seq_dict = dict(zip(subs_target_seq.iloc[:, 0], subs_target_seq.iloc[:, 1])) + subs_target_seq = pd.read_csv('$target_seq_list', sep='\\t', header=None) + subs_target_seq_dict = dict(zip(subs_target_seq.iloc[:, 0], subs_target_seq.iloc[:, 1])) - subs_ref_seq = pd.read_csv('$ref_seq_list', sep='\\t', header=None) - subs_ref_seq_dict = dict(zip(subs_ref_seq.iloc[:, 0], subs_ref_seq.iloc[:, 1])) + subs_ref_seq = pd.read_csv('$ref_seq_list', sep='\\t', header=None) + subs_ref_seq_dict = dict(zip(subs_ref_seq.iloc[:, 0], subs_ref_seq.iloc[:, 1])) - if os.path.getsize('$coloured_bundle_links') == 0: - with open(output_file_name, 'w') as f: - f.write('') - sys.exit(0) - else: - df = pd.read_csv('$coloured_bundle_links', sep=' ', header=None) + if os.path.getsize('$coloured_bundle_links') == 0: + with open(output_file_name, 'w') as f: + f.write('') + sys.exit(0) + else: + df = pd.read_csv('$coloured_bundle_links', sep=' ', header=None) - df.iloc[:, 3] = df.iloc[:, 3].replace(subs_target_seq_dict, regex=False) - df.iloc[:, 0] = df.iloc[:, 0].replace(subs_ref_seq_dict, regex=False) + df.iloc[:, 3] = df.iloc[:, 3].replace(subs_target_seq_dict, regex=False) + df.iloc[:, 0] = df.iloc[:, 0].replace(subs_ref_seq_dict, regex=False) - df.to_csv(output_file_name, sep=' ', index=False, header=None) - """ + df.to_csv(output_file_name, sep=' ', index=False, header=None) + """ } process RELABEL_FASTA_LEN { @@ -373,31 +392,31 @@ process RELABEL_FASTA_LEN { container "docker.io/gallvp/python3npkgs:v0.4" input: - tuple val(target_on_ref), path(target_seq_lengths), path(ref_seq_lengths), path(target_seq_list), path(ref_seq_list) + tuple val(target_on_ref), path(target_seq_lengths), path(ref_seq_lengths), path(target_seq_list), path(ref_seq_list) output: - tuple val(target_on_ref), path("relabeld.target.seq.lengths"), path("relabeld.ref.seq.lengths"), emit: relabeled_seq_lengths + tuple val(target_on_ref), path("relabeld.target.seq.lengths"), path("relabeld.ref.seq.lengths"), emit: relabeled_seq_lengths script: - """ - #!/usr/bin/env python + """ + #!/usr/bin/env python - import pandas as pd + import pandas as pd - subs_target_seq = pd.read_csv('$target_seq_list', sep='\\t', header=None) - subs_target_seq_dict = dict(zip(subs_target_seq.iloc[:, 0], subs_target_seq.iloc[:, 1])) + subs_target_seq = pd.read_csv('$target_seq_list', sep='\\t', header=None) + subs_target_seq_dict = dict(zip(subs_target_seq.iloc[:, 0], subs_target_seq.iloc[:, 1])) - subs_ref_seq = pd.read_csv('$ref_seq_list', sep='\\t', header=None) - subs_ref_seq_dict = dict(zip(subs_ref_seq.iloc[:, 0], subs_ref_seq.iloc[:, 1])) + subs_ref_seq = pd.read_csv('$ref_seq_list', sep='\\t', header=None) + subs_ref_seq_dict = dict(zip(subs_ref_seq.iloc[:, 0], subs_ref_seq.iloc[:, 1])) - df_target_seq_lengths = pd.read_csv('$target_seq_lengths', sep='\\t', header=None) - df_target_seq_lengths.iloc[:, 0] = df_target_seq_lengths.iloc[:, 0].replace(subs_target_seq_dict, regex=False) - df_target_seq_lengths.to_csv("relabeld.target.seq.lengths", sep='\\t', index=False, header=None) + df_target_seq_lengths = pd.read_csv('$target_seq_lengths', sep='\\t', header=None) + df_target_seq_lengths.iloc[:, 0] = df_target_seq_lengths.iloc[:, 0].replace(subs_target_seq_dict, regex=False) + df_target_seq_lengths.to_csv("relabeld.target.seq.lengths", sep='\\t', index=False, header=None) - df_ref_seq_lengths = pd.read_csv('$ref_seq_lengths', sep='\\t', header=None) - df_ref_seq_lengths.iloc[:, 0] = df_ref_seq_lengths.iloc[:, 0].replace(subs_ref_seq_dict, regex=False) - df_ref_seq_lengths.to_csv("relabeld.ref.seq.lengths", sep='\\t', index=False, header=None) - """ + df_ref_seq_lengths = pd.read_csv('$ref_seq_lengths', sep='\\t', header=None) + df_ref_seq_lengths.iloc[:, 0] = df_ref_seq_lengths.iloc[:, 0].replace(subs_ref_seq_dict, regex=False) + df_ref_seq_lengths.to_csv("relabeld.ref.seq.lengths", sep='\\t', index=False, header=None) + """ } process SPLIT_BUNDLE_FILE_BY_TARGET_SEQS { @@ -409,25 +428,25 @@ process SPLIT_BUNDLE_FILE_BY_TARGET_SEQS { 'quay.io/nf-core/ubuntu:20.04' }" input: - tuple val(target_on_ref), path(coloured_bundle_links) + tuple val(target_on_ref), path(coloured_bundle_links) output: - tuple val(target_on_ref), path("*.split.bundle.txt") + tuple val(target_on_ref), path("*.split.bundle.txt"), emit: split_file script: - """ - if [[ "${params.synteny.plot_1_vs_all}" = "1" ]];then - target_seqs=(\$(awk '{print \$4}' $coloured_bundle_links | sort | uniq)) - - for i in "\${!target_seqs[@]}" - do - target_seq=\${target_seqs[\$i]} - awk -v seq="\$target_seq" '\$4==seq {print \$0}' $coloured_bundle_links > "${target_on_ref}.\${target_seq}.split.bundle.txt" - done - fi - - cat $coloured_bundle_links > "${target_on_ref}.all.split.bundle.txt" - """ + """ + if [[ "${params.synteny.plot_1_vs_all}" = "1" ]];then + target_seqs=(\$(awk '{print \$4}' $coloured_bundle_links | sort | uniq)) + + for i in "\${!target_seqs[@]}" + do + target_seq=\${target_seqs[\$i]} + awk -v seq="\$target_seq" '\$4==seq {print \$0}' $coloured_bundle_links > "${target_on_ref}.\${target_seq}.split.bundle.txt" + done + fi + + cat $coloured_bundle_links > "${target_on_ref}.all.split.bundle.txt" + """ } process GENERATE_KARYOTYPE { @@ -439,43 +458,43 @@ process GENERATE_KARYOTYPE { 'quay.io/nf-core/ubuntu:20.04' }" input: - tuple val(target_on_ref), val(seq_tag), path(split_bundle_file), path(target_seq_len), path(ref_seq_len) + tuple val(target_on_ref), val(seq_tag), path(split_bundle_file), path(target_seq_len), path(ref_seq_len) output: - tuple val("${target_on_ref}.${seq_tag}"), path("*.karyotype") + tuple val("${target_on_ref}.${seq_tag}"), path("*.karyotype"), emit: karyotype script: - """ - ref_seqs=(\$(awk '{print \$1}' $split_bundle_file | sort | uniq)) + """ + ref_seqs=(\$(awk '{print \$1}' $split_bundle_file | sort | uniq)) - if [ \${#ref_seqs[@]} -eq 0 ]; then - touch "${target_on_ref}.${seq_tag}.karyotype" - exit 0 - fi + if [ \${#ref_seqs[@]} -eq 0 ]; then + touch "${target_on_ref}.${seq_tag}.karyotype" + exit 0 + fi - tmp_file=\$(mktemp) - printf '%s\\n' "\${ref_seqs[@]}" > "\$tmp_file" + tmp_file=\$(mktemp) + printf '%s\\n' "\${ref_seqs[@]}" > "\$tmp_file" - if [[ $seq_tag = "all" ]];then - cat $target_seq_len > filtered.target.seq.len - else - grep -w "$seq_tag" $target_seq_len > filtered.target.seq.len - fi - cat filtered.target.seq.len | awk '{print \$1,\$2,"grey"}' OFS="\\t" > colored.filtered.target.seq.len + if [[ $seq_tag = "all" ]];then + cat $target_seq_len > filtered.target.seq.len + else + grep -w "$seq_tag" $target_seq_len > filtered.target.seq.len + fi + cat filtered.target.seq.len | awk '{print \$1,\$2,"grey"}' OFS="\\t" > colored.filtered.target.seq.len - grep -w -f "\$tmp_file" $ref_seq_len > filtered.ref.seq.len - cat filtered.ref.seq.len | awk '{print \$1,\$2,"black"}' OFS="\\t" > colored.filtered.ref.seq.len + grep -w -f "\$tmp_file" $ref_seq_len > filtered.ref.seq.len + cat filtered.ref.seq.len | awk '{print \$1,\$2,"black"}' OFS="\\t" > colored.filtered.ref.seq.len - cat colored.filtered.ref.seq.len | sort -k1V > merged.seq.lengths - cat colored.filtered.target.seq.len | sort -k1Vr >> merged.seq.lengths - sed -i '/^\$/d' merged.seq.lengths + cat colored.filtered.ref.seq.len | sort -k1V > merged.seq.lengths + cat colored.filtered.target.seq.len | sort -k1Vr >> merged.seq.lengths + sed -i '/^\$/d' merged.seq.lengths - cat merged.seq.lengths \ - | awk '{print "chr -",\$1,\$1,"0",\$2-1,\$3}' OFS="\\t" \ - > "${target_on_ref}.${seq_tag}.karyotype" + cat merged.seq.lengths \ + | awk '{print "chr -",\$1,\$1,"0",\$2-1,\$3}' OFS="\\t" \ + > "${target_on_ref}.${seq_tag}.karyotype" - rm "\$tmp_file" - """ + rm "\$tmp_file" + """ } process CIRCOS { @@ -483,131 +502,137 @@ process CIRCOS { label "process_single" container "docker.io/gallvp/circos-tools:v0.23-1_ps" - publishDir "${params.outdir}/synteny/${target_on_ref_seq}", mode: 'copy' input: - tuple val(target_on_ref_seq), path(karyotype), path(bundle_file) + tuple val(target_on_ref_seq), path(karyotype), path(bundle_file) output: - path "*.svg", emit: svg_file - path "*.png", emit: png_file - path "bundled.links.tsv", emit: bundled_links_tsv - path "circos.conf", emit: circos_conf - path "karyotype.tsv", emit: karyotype_tsv + path "*.svg", emit: svg_file + path "*.png", emit: png_file + path "bundled.links.tsv", emit: bundled_links_tsv + path "circos.conf", emit: circos_conf + path "karyotype.tsv", emit: karyotype_tsv + path "versions.yml", emit: versions script: - """ - - links_count=\$(wc -l < "$bundle_file") - max_links=20000 - if [ "\$links_count" -gt "\$max_links" ]; then - echo "Link count exceeded \$max_links for ${bundle_file}." - echo "Try to shrink the number of links by increasing the max_gap and min_bundle_size options in the config file." - exit 1 - fi - - cat $karyotype > "karyotype.tsv" - cat $bundle_file | awk '{print \$1,\$2,\$3,\$4,\$5,\$6,\$7}' OFS="\\t" > bundled.links.tsv - - num_sequences=\$(cat $karyotype | wc -l) - if (( \$num_sequences <= 10 )); then - label_font_size=40 - elif (( \$num_sequences <= 30 )); then - label_font_size=30 - else - label_font_size=15 - fi - - if (( \$num_sequences <= 10 )); then - ticks_config=" - radius = dims(ideogram,radius_outer) - orientation = out - label_multiplier = 1e-6 - color = black - thickness = 5p - label_offset = 5p - - spacing = 0.5u - size = 10p - show_label = yes - label_size = 20p - format = %.1f - - - spacing = 1.0u - size = 15p - show_label = yes - label_size = 30p - format = %.1f - - " - - label_offset=" + 120p" - else - ticks_config="" - - label_offset=" + 25p" - fi - - cat <<- EOF > circos.conf - # circos.conf - karyotype = $karyotype - - - - default = 0.005r - - - radius = 0.8r - thickness = 25p - fill = yes - stroke_thickness = 0 - + """ + + links_count=\$(wc -l < "$bundle_file") + max_links=20000 + if [ "\$links_count" -gt "\$max_links" ]; then + echo "Link count exceeded \$max_links for ${bundle_file}." + echo "Try to shrink the number of links by increasing the max_gap and min_bundle_size options in the config file." + exit 1 + fi + + cat $karyotype > "karyotype.tsv" + cat $bundle_file | awk '{print \$1,\$2,\$3,\$4,\$5,\$6,\$7}' OFS="\\t" > bundled.links.tsv + + num_sequences=\$(cat $karyotype | wc -l) + if (( \$num_sequences <= 10 )); then + label_font_size=40 + elif (( \$num_sequences <= 30 )); then + label_font_size=30 + else + label_font_size=15 + fi + + if (( \$num_sequences <= 10 )); then + ticks_config=" + radius = dims(ideogram,radius_outer) + orientation = out + label_multiplier = 1e-6 + color = black + thickness = 5p + label_offset = 5p + + spacing = 0.5u + size = 10p + show_label = yes + label_size = 20p + format = %.1f + + + spacing = 1.0u + size = 15p show_label = yes - label_font = default - label_radius = dims(ideogram,radius_outer)\$label_offset - label_size = \$label_font_size - label_parallel = yes - - - - radius = 0.99r - crest = 1 - ribbon = yes - flat = yes - stroke_thickness = 0 - color = grey_a3 - - bezier_radius = 0r - bezier_radius_purity = 0.5 - - file = bundled.links.tsv - - - - show_ticks = yes - show_tick_labels = yes - chromosomes_units = 1000000 - chromosomes_display_default = yes - - \$ticks_config - - - <> - - <> - <> -EOF - - if [ ! -s $karyotype ]; then - touch "${target_on_ref_seq}.svg" - touch "${target_on_ref_seq}.png" - exit 0 - fi - - circos - - mv circos.svg "${target_on_ref_seq}.svg" - mv circos.png "${target_on_ref_seq}.png" - """ + label_size = 30p + format = %.1f + + " + + label_offset=" + 120p" + else + ticks_config="" + + label_offset=" + 25p" + fi + + cat <<-END_CONF > circos.conf + # circos.conf + karyotype = $karyotype + + + + default = 0.005r + + + radius = 0.8r + thickness = 25p + fill = yes + stroke_thickness = 0 + + show_label = yes + label_font = default + label_radius = dims(ideogram,radius_outer)\$label_offset + label_size = \$label_font_size + label_parallel = yes + + + + radius = 0.99r + crest = 1 + ribbon = yes + flat = yes + stroke_thickness = 0 + color = grey_a3 + + bezier_radius = 0r + bezier_radius_purity = 0.5 + + file = bundled.links.tsv + + + + show_ticks = yes + show_tick_labels = yes + chromosomes_units = 1000000 + chromosomes_display_default = yes + + \$ticks_config + + + <> + + <> + <> + END_CONF + + if [ ! -s $karyotype ]; then + touch "${target_on_ref_seq}.svg" + touch "${target_on_ref_seq}.png" + exit 0 + fi + + circos + + mv circos.svg "${target_on_ref_seq}.svg" + mv circos.png "${target_on_ref_seq}.png" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + circos: \$(circos -version | awk '{print \$2}' FS='|' | tr -d '[:space:]') + perl: \$(circos -version | awk '{print \$4}' FS='|' | tr -d '[:space:]Perl') + END_VERSIONS + """ } diff --git a/subworkflows/local/tidk.nf b/subworkflows/local/tidk.nf deleted file mode 100644 index dd5728ce..00000000 --- a/subworkflows/local/tidk.nf +++ /dev/null @@ -1,212 +0,0 @@ -nextflow.enable.dsl=2 - -workflow TIDK { - take: - tuple_of_hap_file - - main: - if (!params.tidk.skip) { - GET_APRIORI_SEQUENCE() - .set { ch_apriori_sequence } - - SORT_AND_FILTER_BY_SEQ_LENGTH(tuple_of_hap_file) - .set { ch_sorted_hap_file } - - EXPLORE_REPEAT_SEQ(tuple_of_hap_file) - .set { ch_explored_repeat_seq } - - ch_explored_repeat_seq - .join( - ch_sorted_hap_file - ) - | SEARCH_A_POSTERIORI_REPEAT_SEQ - | PLOT_A_POSTERIORI_REPEAT_SEQ - | collect - | set { ch_list_of_a_posteriori_tidk_plots } - - SEARCH_A_PRIORI_REPEAT_SEQ(ch_sorted_hap_file) - | PLOT_A_PRIORI_REPEAT_SEQ - | collect - | set { ch_list_of_a_priori_tidk_plots } - - - ch_list_of_a_posteriori_tidk_plots - .mix(ch_list_of_a_priori_tidk_plots) - .mix( - ch_explored_repeat_seq - .map { - it[1] // a_posteriori sequence - } - ) - .mix(ch_apriori_sequence) - .collect() - .set { ch_list_of_tidk_plots } - } - else { - ch_list_of_tidk_plots = Channel.of([]) - } - - emit: - list_of_plots = ch_list_of_tidk_plots -} - -process GET_APRIORI_SEQUENCE { - tag "setup" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/ubuntu:20.04': - 'quay.io/nf-core/ubuntu:20.04' }" - - output: - path("a_priori.sequence") - - script: - """ - echo "${params.tidk.repeat_seq}" >> a_priori.sequence - """ -} - -process SORT_AND_FILTER_BY_SEQ_LENGTH { - tag "${hap_name}" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/seqkit:2.3.1--h9ee0642_0': - 'quay.io/biocontainers/seqkit:2.3.1--h9ee0642_0' }" - - input: - tuple val(hap_name), path(fasta_file) - - output: - tuple val(hap_name), path("${hap_name}.seqkit.sort.fasta") - - script: - """ - if [[ "${params.tidk.filter_by_size}" = "1" ]];then - seqkit seq -m ${params.tidk.filter_size_bp} $fasta_file > filtered.file.fasta - else - cat $fasta_file > filtered.file.fasta - fi - - cat filtered.file.fasta \ - | seqkit sort --quiet --reverse --by-length \ - > "${hap_name}.seqkit.sort.fasta" - """ -} - -process SEARCH_A_PRIORI_REPEAT_SEQ { - tag "${hap_name}" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/tidk:0.2.31--h87f3376_0': - 'quay.io/biocontainers/tidk:0.2.31--h87f3376_0' }" - publishDir params.outdir, mode: 'copy' - - input: - tuple val(hap_name), path(fasta_file) - - output: - tuple val(hap_name), path("tidk/${hap_name}.a_priori.tidk.search*.tsv") - - script: - """ - tidk search --string "${params.tidk.repeat_seq}" --output "${hap_name}.a_priori.tidk.search" --dir tidk --extension "tsv" "${fasta_file}" - """ -} - -process EXPLORE_REPEAT_SEQ { - tag "${hap_name}" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/tidk:0.2.31--h87f3376_0': - 'quay.io/biocontainers/tidk:0.2.31--h87f3376_0' }" - publishDir "${params.outdir}/tidk", mode: 'copy' - - input: - tuple val(hap_name), path(fasta_file) - - output: - tuple val(hap_name), path("${hap_name}.a_posteriori.sequence") - - script: - """ - tidk explore --minimum 5 --maximum 30 "${fasta_file}" > ${hap_name}.tidk.explore.txt - cat ${hap_name}.tidk.explore.txt | sed -n 2p | awk '{print \$1;}' > "${hap_name}.a_posteriori.sequence" - """ -} - -process SEARCH_A_POSTERIORI_REPEAT_SEQ { - tag "${hap_name}" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/tidk:0.2.31--h87f3376_0': - 'quay.io/biocontainers/tidk:0.2.31--h87f3376_0' }" - publishDir params.outdir, mode: 'copy' - - input: - tuple val(hap_name), path(hap_explored_sequence), path(fasta_file) - - output: - tuple val(hap_name), path("tidk/${hap_name}.a_posteriori.tidk.search*.tsv") - - script: - """ - if [ -s ${hap_name}.a_posteriori.sequence ]; then - xyz=`cat ${hap_name}.a_posteriori.sequence` - tidk search --string "\${xyz}" --output "${hap_name}.a_posteriori.tidk.search" --dir tidk --extension "tsv" "${fasta_file}" - else - mkdir tidk - touch tidk/${hap_name}.a_posteriori.tidk.search.empty.tsv - fi - """ -} - -process PLOT_A_PRIORI_REPEAT_SEQ { - tag "${hap_name}" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/tidk:0.2.31--h87f3376_0': - 'quay.io/biocontainers/tidk:0.2.31--h87f3376_0' }" - publishDir "${params.outdir}/tidk", mode: 'copy' - - input: - tuple val(hap_name), path(tsv_file) - - output: - path "${hap_name}_a_priori.tidk.plot*.svg" - - script: - """ - tidk plot --tsv "$tsv_file" --output "${hap_name}_a_priori.tidk.plot" - """ -} - -process PLOT_A_POSTERIORI_REPEAT_SEQ { - tag "${hap_name}" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/tidk:0.2.31--h87f3376_0': - 'quay.io/biocontainers/tidk:0.2.31--h87f3376_0' }" - publishDir "${params.outdir}/tidk", mode: 'copy' - - input: - tuple val(hap_name), path(tsv_file) - - output: - path "${hap_name}_a_posteriori.tidk.plot*.svg" - - script: - """ - if [ -s ${tsv_file} ]; then - tidk plot --tsv "$tsv_file" --output "${hap_name}_a_posteriori.tidk.plot" - else - touch ${hap_name}_a_posteriori.tidk.plot.empty.svg - fi - """ -} diff --git a/subworkflows/local/validate_fasta.nf b/subworkflows/local/validate_fasta.nf deleted file mode 100644 index 796911b8..00000000 --- a/subworkflows/local/validate_fasta.nf +++ /dev/null @@ -1,52 +0,0 @@ -nextflow.enable.dsl=2 - -include { GZIP_FASTA } from '../../modules/local/gzip_fasta' - -workflow VALIDATE_FASTA { - take: - tuple_of_tag_file - - main: - tuple_of_tag_file - | GZIP_FASTA - | set { ch_tuple_tag_extracted_file } - - ch_tuple_tag_extracted_file - | RUN_VALIDATOR - | map { - def literals = it.split(":") - - [literals[1]] // [tag] - } - | join( - ch_tuple_tag_extracted_file - ) - | set { ch_tuple_tag_valid_fasta } - - emit: - tuple_tag_valid_fasta = ch_tuple_tag_valid_fasta -} - -process RUN_VALIDATOR { - tag "${tag_label}" - label "process_single" - - container "docker.io/gallvp/fasta_validator:a6a2ec1_ps" - - input: - tuple val(tag_label), path(fasta_file) - - output: - stdout - - script: - """ - fasta_validate -v $fasta_file >/dev/null - - # If invalid, the above command will fail and - # the NXF error startegy will kick in. - # Otherwise, pass the is_valid status to stdout - - echo -n "VALIDATE_FASTA:$tag_label:VALID" - """ -} diff --git a/subworkflows/local/validate_gff3.nf b/subworkflows/local/validate_gff3.nf deleted file mode 100644 index d29ca495..00000000 --- a/subworkflows/local/validate_gff3.nf +++ /dev/null @@ -1,143 +0,0 @@ -nextflow.enable.dsl=2 - -workflow VALIDATE_GFF3 { - take: - tuple_of_tag_gff3_file - tuple_of_tag_fasta_file - - main: - tuple_of_tag_gff3_file - | GZIP_GFF3 - | FORMAT_GFF3 - | set { ch_tuple_tag_extracted_file } - - ch_tuple_tag_extracted_file - | RUN_VALIDATOR - | map { - def literals = it.split(":") - - [literals[1]] // [tag] - } - | join( - ch_tuple_tag_extracted_file - ) - | set { ch_tuple_tag_after_validator } - - - tuple_of_tag_fasta_file - | cross(ch_tuple_tag_after_validator) - | map { - [it[0][0], it[1][1], it[0][1]] // [tag, gff3, fasta] - } - | CHECK_FASTA_GFF3_CORRESPONDENCE - | map { - def literals = it.split(":") - - [literals[1]] // [tag] - } - | join( - ch_tuple_tag_extracted_file - ) - | set { ch_tuple_tag_valid_gff3 } - - emit: - tuple_tag_valid_gff3 = ch_tuple_tag_valid_gff3 -} - -process GZIP_GFF3 { - tag "${tag_label}" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/ubuntu:20.04': - 'quay.io/nf-core/ubuntu:20.04' }" - - input: - tuple val(tag_label), path(gff3_file) - - output: - tuple val(tag_label), path("*.gzip.gff3") - - script: - """ - input_file_name_var="\$(basename $gff3_file .gz)" - output_file_name="\${input_file_name_var%.*}.gzip.gff3" - - gzip -cdf "$gff3_file" > "\$output_file_name" - """ -} - -process FORMAT_GFF3 { - tag "${tag_label}" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/genometools-genometools:1.6.2--py310he7ef181_3': - 'quay.io/biocontainers/genometools-genometools:1.6.2--py310he7ef181_3' }" - - input: - tuple val(tag_label), path(gff3_file) - - output: - tuple val(tag_label), path("*.gt.gff3") - - script: - """ - output_file_name="\$(basename $gff3_file .gzip.gff3).gt.gff3" - - gt gff3 -tidy -retainids "$gff3_file" \ - > "\$output_file_name" - """ -} - -process RUN_VALIDATOR { - tag "${tag_label}" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/genometools-genometools:1.6.2--py310he7ef181_3': - 'quay.io/biocontainers/genometools-genometools:1.6.2--py310he7ef181_3' }" - - input: - tuple val(tag_label), path(gff3_file) - - output: - stdout - - script: - """ - gt gff3validator "$gff3_file" >/dev/null - - # If invalid, the above command will fail and - # the NXF error startegy will kick in. - # Otherwise, pass the is_valid status to stdout - - echo -n "VALIDATE_GFF3:$tag_label:VALID" - """ -} - -process CHECK_FASTA_GFF3_CORRESPONDENCE { - tag "${tag_label}" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1': - 'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }" - - input: - tuple val(tag_label), path(gff3_file), path(fasta_file) - - output: - stdout - - script: - """ - check_gff3_fasta_corresp_3031aca.sh "$fasta_file" "$gff3_file" - - # If invalid, the above command will fail and - # the NXF error startegy will kick in. - # Otherwise, pass the is_valid status to stdout - - echo -n "CHECK_FASTA_GFF3_CORRESPONDENCE:$tag_label:VALID" - """ -} diff --git a/subworkflows/pfr/gff3_validate/main.nf b/subworkflows/pfr/gff3_validate/main.nf new file mode 100644 index 00000000..5437c5a6 --- /dev/null +++ b/subworkflows/pfr/gff3_validate/main.nf @@ -0,0 +1,61 @@ +include { GT_GFF3 } from '../../../modules/pfr/gt/gff3/main' +include { GT_GFF3VALIDATOR } from '../../../modules/pfr/gt/gff3validator/main' +include { CUSTOM_CHECKGFF3FASTACORRESPONDENCE } from '../../../modules/pfr/custom/checkgff3fastacorrespondence/main' + +workflow GFF3_VALIDATE { + + take: + ch_gff3 // channel: [ val(meta), gff3 ] + ch_fasta // channel: [ val(meta), fasta ] + + main: + + ch_versions = Channel.empty() + + // MODULE: GT_GFF3 + GT_GFF3 ( ch_gff3 ) + ch_versions = ch_versions.mix(GT_GFF3.out.versions.first()) + + // MODULE: GT_GFF3VALIDATOR + GT_GFF3VALIDATOR ( GT_GFF3.out.gt_gff3 ) + ch_versions = ch_versions.mix(GT_GFF3VALIDATOR.out.versions.first()) + + // MODULE: CUSTOM_CHECKGFF3FASTACORRESPONDENCE + GT_GFF3VALIDATOR.out.success_log + | join ( + GT_GFF3.out.gt_gff3 + ) + | map { meta, log, gff3 -> [ meta, gff3 ] } + | join ( + ch_fasta + ) + | set { ch_gff3_fasta } + + CUSTOM_CHECKGFF3FASTACORRESPONDENCE ( + ch_gff3_fasta.map { meta, gff3, fasta -> [ meta, gff3 ] }, + ch_gff3_fasta.map { meta, gff3, fasta -> fasta } + ) + + ch_versions = ch_versions.mix(CUSTOM_CHECKGFF3FASTACORRESPONDENCE.out.versions.first()) + + CUSTOM_CHECKGFF3FASTACORRESPONDENCE.out.success_log + | join ( + ch_gff3_fasta.map { meta, gff3, fasta -> [ meta, gff3 ] } + ) + | map { meta, log, gff3 -> [ meta, gff3 ] } + | set { ch_valid_gff3 } + + GT_GFF3.out.error_log + | mix ( + GT_GFF3VALIDATOR.out.error_log + ) + | mix ( + CUSTOM_CHECKGFF3FASTACORRESPONDENCE.out.error_log + ) + | set { ch_log_for_invalid_gff3 } + + emit: + valid_gff3 = ch_valid_gff3 // channel: [ val(meta), gff3 ] + log_for_invalid_gff3 = ch_log_for_invalid_gff3 // channel: [ val(meta), log ] + versions = ch_versions // channel: [ versions.yml ] +} diff --git a/subworkflows/pfr/gff3_validate/meta.yml b/subworkflows/pfr/gff3_validate/meta.yml new file mode 100644 index 00000000..5dea12a5 --- /dev/null +++ b/subworkflows/pfr/gff3_validate/meta.yml @@ -0,0 +1,50 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: gff3_validate +description: | + Validates a gff3 file using GenomeTools gt-gff3, gt-gff3validator and + checks its correspondence with a fasta file +keywords: + - genome + - gff3 + - annotation + - validation +components: + - gt/gff3 + - gt/gff3validator + - custom/checkgff3fastacorrespondence +input: + - ch_gff3: + type: file + description: | + Input channel containing a gff3 file + Structure: [ val(meta), path(gff3) ] + pattern: "*.{gff,gff3}" + - ch_fasta: + type: file + description: | + Input channel containing a fasta file + Structure: [ val(meta), path(fasta) ] + pattern: "*.{fsa,fa,fasta}" +output: + - valid_gff3: + type: file + description: | + Valid gff3 file + Structure: [ val(meta), path(gff3) ] + pattern: "*.gff3" + - log_for_invalid_gff3: + type: file + description: | + Error log if the gff3 file is invalid + Structure: [ val(meta), path(gff3) ] + pattern: "*.gff3" + - versions: + type: file + description: | + File containing software versions + Structure: [ path(versions.yml) ] + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/subworkflows/pfr/gff3_validate/tests/main.nf.test b/subworkflows/pfr/gff3_validate/tests/main.nf.test new file mode 100644 index 00000000..fc1d7622 --- /dev/null +++ b/subworkflows/pfr/gff3_validate/tests/main.nf.test @@ -0,0 +1,86 @@ +nextflow_workflow { + + name "Test Workflow GFF3_VALIDATE" + script "../main.nf" + workflow "GFF3_VALIDATE" + config "./nextflow.config" + + tag "subworkflows" + tag "subworkflows_nfcore" + tag "subworkflows/gff3_validate" + tag "gff3_validate" + tag "gt" + tag "gt/gff3'" + tag "gt/gff3validator'" + tag "custom" + tag "custom/checkgff3fastacorrespondence'" + + test("sarscov2-genome_gff3-genome_fasta-all_pass") { + + when { + workflow { + """ + input[0] = Channel.of([ [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) + ]) + input[1] = Channel.of([ [ id:'test' ], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ]) + """ + } + } + + then { + assertAll( + { assert workflow.success}, + { assert snapshot(workflow.out).match()} + ) + } + } + + test("homo_sapiens-genome_bed-genome_fasta-gt_gff3_fail") { + + when { + workflow { + """ + input[0] = Channel.of([ [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true) + ]) + input[1] = Channel.of([ [ id:'test' ], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ]) + """ + } + } + + then { + assertAll( + { assert workflow.success}, + { assert snapshot(workflow.out).match()} + ) + } + } + + test("sarscov2-genome_gff3-homo_sapiens-genome_fasta-correspondence_fail") { + + when { + workflow { + """ + input[0] = Channel.of([ [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) + ]) + input[1] = Channel.of([ [ id:'test' ], + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ]) + """ + } + } + + then { + assertAll( + { assert workflow.success}, + { assert snapshot(workflow.out).match()} + ) + } + } +} \ No newline at end of file diff --git a/subworkflows/pfr/gff3_validate/tests/main.nf.test.snap b/subworkflows/pfr/gff3_validate/tests/main.nf.test.snap new file mode 100644 index 00000000..4d2a59b2 --- /dev/null +++ b/subworkflows/pfr/gff3_validate/tests/main.nf.test.snap @@ -0,0 +1,115 @@ +{ + "sarscov2-genome_gff3-genome_fasta-all_pass": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.gt.gff3:md5,2ae900237ace415557b8735fac088b85" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,10fe5c201e5fcddb52c3607ab3fdfb34", + "versions.yml:md5,856745cef2fff087e50ea4c0ffa3addd", + "versions.yml:md5,a89255422a163684b0c80ebdd8ad28ae" + ], + "log_for_invalid_gff3": [ + + ], + "valid_gff3": [ + [ + { + "id": "test" + }, + "test.gt.gff3:md5,2ae900237ace415557b8735fac088b85" + ] + ], + "versions": [ + "versions.yml:md5,10fe5c201e5fcddb52c3607ab3fdfb34", + "versions.yml:md5,856745cef2fff087e50ea4c0ffa3addd", + "versions.yml:md5,a89255422a163684b0c80ebdd8ad28ae" + ] + } + ], + "timestamp": "2023-12-07T10:33:21.09887" + }, + "homo_sapiens-genome_bed-genome_fasta-gt_gff3_fail": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "test" + }, + "test.error.log:md5,c096494c3cd02864eb54434c294ba382" + ] + ], + "2": [ + "versions.yml:md5,a89255422a163684b0c80ebdd8ad28ae" + ], + "log_for_invalid_gff3": [ + [ + { + "id": "test" + }, + "test.error.log:md5,c096494c3cd02864eb54434c294ba382" + ] + ], + "valid_gff3": [ + + ], + "versions": [ + "versions.yml:md5,a89255422a163684b0c80ebdd8ad28ae" + ] + } + ], + "timestamp": "2023-12-07T10:35:26.549003" + }, + "sarscov2-genome_gff3-homo_sapiens-genome_fasta-correspondence_fail": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "test" + }, + "test.error.log:md5,67686ea1ef271821f1218a8fe0207e1f" + ] + ], + "2": [ + "versions.yml:md5,10fe5c201e5fcddb52c3607ab3fdfb34", + "versions.yml:md5,856745cef2fff087e50ea4c0ffa3addd", + "versions.yml:md5,a89255422a163684b0c80ebdd8ad28ae" + ], + "log_for_invalid_gff3": [ + [ + { + "id": "test" + }, + "test.error.log:md5,67686ea1ef271821f1218a8fe0207e1f" + ] + ], + "valid_gff3": [ + + ], + "versions": [ + "versions.yml:md5,10fe5c201e5fcddb52c3607ab3fdfb34", + "versions.yml:md5,856745cef2fff087e50ea4c0ffa3addd", + "versions.yml:md5,a89255422a163684b0c80ebdd8ad28ae" + ] + } + ], + "timestamp": "2023-12-07T10:35:32.53584" + } +} \ No newline at end of file diff --git a/subworkflows/pfr/gff3_validate/tests/nextflow.config b/subworkflows/pfr/gff3_validate/tests/nextflow.config new file mode 100644 index 00000000..d07a8881 --- /dev/null +++ b/subworkflows/pfr/gff3_validate/tests/nextflow.config @@ -0,0 +1,6 @@ +process { + + withName: GT_GFF3 { + ext.args = '-tidy -retainids -addintrons' + } +} diff --git a/subworkflows/pfr/gff3_validate/tests/tags.yml b/subworkflows/pfr/gff3_validate/tests/tags.yml new file mode 100644 index 00000000..60ffbf0c --- /dev/null +++ b/subworkflows/pfr/gff3_validate/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/gff3_validate: + - subworkflows/pfr/gff3_validate/** diff --git a/version_check.sh b/version_check.sh index 1af5c650..ce55eaba 100755 --- a/version_check.sh +++ b/version_check.sh @@ -1,10 +1,10 @@ #!/usr/bin/env bash -report_version=$(sed -n '/"SELF": /s/"SELF": "v//p' ./bin/assembly_qc_report_943e0fb.py | sed 's/",//' | tr -d '[:space:]') +config_version=$(sed -n '/version/s/version//p' nextflow.config | tr -d " ='[:space:]}") cff_version=$(sed -n '/^version: /s/version: //p' CITATION.cff | tr -d '[:space:]') -if [[ $report_version != $cff_version ]]; then - echo 'report_version != cff_version' +if [[ $config_version != $cff_version ]]; then + echo 'config_version != cff_version' exit 1 fi @@ -12,12 +12,12 @@ fi readme_citation_text=$(grep -A10 '## Citations' README.md) -if [[ ! $readme_citation_text =~ "($report_version)" ]]; then +if [[ ! $readme_citation_text =~ "($config_version)" ]]; then echo 'Failed to match README version' exit 1 fi # Check CHANGELOG version -grep "Version $report_version" CHANGELOG.md >/dev/null \ +grep "Version $config_version" CHANGELOG.md >/dev/null \ || (echo 'Failed to match CHANGELOG version'; exit 1) diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index 1284619e..b655c82f 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -3,168 +3,299 @@ nextflow.enable.dsl=2 include { validateParams } from '../modules/local/utils' include { jsonifyParams } from '../modules/local/utils' -include { VALIDATE_FASTA } from '../subworkflows/local/validate_fasta' -include { VALIDATE_GFF3 } from '../subworkflows/local/validate_gff3' +include { GUNZIP as GUNZIP_FASTA } from '../modules/nf-core/gunzip/main' +include { FASTAVALIDATOR } from '../modules/nf-core/fastavalidator/main' +include { GUNZIP as GUNZIP_GFF3 } from '../modules/nf-core/gunzip/main' +include { GFF3_VALIDATE } from '../subworkflows/pfr/gff3_validate/main' +include { GT_STAT } from '../modules/pfr/gt/stat/main' +include { NCBI_FCS_ADAPTOR } from '../modules/local/ncbi_fcs_adaptor' +include { NCBI_FCS_GX } from '../subworkflows/local/ncbi_fcs_gx' +include { ASSEMBLATHON_STATS } from '../modules/local/assemblathon_stats' include { BUSCO } from '../subworkflows/local/busco' include { FASTA_EXPLORE_SEARCH_PLOT_TIDK } from '../subworkflows/nf-core/fasta_explore_search_plot_tidk/main' include { FASTA_LTRRETRIEVER_LAI } from '../subworkflows/pfr/fasta_ltrretriever_lai/main' include { KRAKEN2 } from '../subworkflows/local/kraken2' -include { NCBI_FCS_ADAPTOR } from '../subworkflows/local/ncbi_fcs_adaptor' -include { NCBI_FCS_GX } from '../subworkflows/local/ncbi_fcs_gx' include { FASTQ_TRIM_FASTP_FASTQC } from '../subworkflows/nf-core/fastq_trim_fastp_fastqc/main' include { HIC_CONTACT_MAP } from '../subworkflows/local/hic_contact_map' include { SYNTENY } from '../subworkflows/local/synteny' - +include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' include { CREATE_REPORT } from '../modules/local/create_report' -include { ASSEMBLATHON_STATS } from '../modules/local/assemblathon_stats' -include { GENOMETOOLS_GT_STAT } from '../modules/local/genometools_gt_stat' -include { BIOCODE_GFF3_STATS } from '../modules/local/biocode_gff3_stats' - validateParams(params) -def paramsAsJSON = jsonifyParams(params) workflow ASSEMBLYQC { - // VALIDATE_FASTA - Channel.fromList(params.target_assemblies) - | map { - [it[0], file(it[1], checkIfExists: true)] // [tag, assembly fasta path] - } - | VALIDATE_FASTA - | set { ch_tag_valid_fasta } + // Input channels + ch_versions = Channel.empty() - // VALIDATE_GFF3 - Channel.fromList(params.assembly_gff3) - | map { - [it[0], file(it[1], checkIfExists: true)] // [tag, assembly gff3 path] - } - | set { ch_tag_gff3_file } + ch_target_assemby_branch = Channel.fromList(params.target_assemblies) + | map { tag, fasta -> + [ [ id: tag ], file(fasta, checkIfExists: true) ] + } + | branch { meta, fasta -> + gz: "$fasta".endsWith(".gz") + rest: !"$fasta".endsWith(".gz") + } - VALIDATE_GFF3(ch_tag_gff3_file, ch_tag_valid_fasta) - | set { ch_tag_valid_gff3 } + ch_assemby_gff3_branch = Channel.fromList(params.assembly_gff3) + | map { tag, gff3 -> + [ [ id: tag ], file(gff3, checkIfExists: true) ] + } + | branch { meta, gff3 -> + gz: "$gff3".endsWith(".gz") + rest: !"$gff3".endsWith(".gz") + } + // MODULE: GUNZIP as GUNZIP_FASTA + GUNZIP_FASTA ( ch_target_assemby_branch.gz ) - // GENOMETOOLS_GT_STAT - ch_tag_valid_gff3 - | GENOMETOOLS_GT_STAT - | collect - | set { ch_genometools_gt_stats } + ch_target_assembly = GUNZIP_FASTA.out.gunzip.mix(ch_target_assemby_branch.rest) + ch_versions = ch_versions.mix(GUNZIP_FASTA.out.versions.first()) - // BIOCODE_GFF3_STATS - ch_tag_valid_gff3 - | BIOCODE_GFF3_STATS - | collect - | set { ch_biocode_gff3_stats } + // MODULE: GUNZIP as GUNZIP_GFF3 + GUNZIP_GFF3 ( ch_assemby_gff3_branch.gz ) + ch_assembly_gff3 = GUNZIP_GFF3.out.gunzip.mix(ch_assemby_gff3_branch.rest) + ch_versions = ch_versions.mix(GUNZIP_GFF3.out.versions.first()) - // NCBI-FCS-ADAPTOR & NCBI-FCS-GX - ch_tag_valid_fasta - | NCBI_FCS_ADAPTOR + // MODULE: FASTAVALIDATOR + FASTAVALIDATOR ( ch_target_assembly ) - NCBI_FCS_GX( - ch_tag_valid_fasta, - params.ncbi_fcs_gx.db_path - ) + ch_valid_target_assembly = ch_target_assembly.join(FASTAVALIDATOR.out.success_log) + | map { meta, fasta, log -> [ meta, fasta ] } + + ch_invalid_assembly_log = FASTAVALIDATOR.out.error_log + | map { meta, error_log -> + log.warn("FASTA validation failed for ${meta.id}\n${error_log.text}") + + [ meta, error_log ] + } - NCBI_FCS_ADAPTOR - .out - .is_clean_status - | join( - NCBI_FCS_GX - .out - .is_clean_status + ch_versions = ch_versions.mix(FASTAVALIDATOR.out.versions.first()) + + // SUBWORKFLOW: GFF3_VALIDATE + GFF3_VALIDATE ( + ch_assembly_gff3, + ch_valid_target_assembly ) - | filter { - it[1] && it[2] // NCBI_FCS_ADAPTOR and NCBI_FCS_GX both report no contamination - } - | join( - ch_tag_valid_fasta + + ch_valid_gff3 = GFF3_VALIDATE.out.valid_gff3 + + ch_invalid_gff3_log = GFF3_VALIDATE.out.log_for_invalid_gff3 + | map { meta, error_log -> + log.warn("GFF3 validation failed for ${meta.id}\n${error_log.text}") + + [ meta, error_log ] + } + + ch_versions = ch_versions.mix(GFF3_VALIDATE.out.versions) + + // MODULE: GT_STAT + GT_STAT ( ch_valid_gff3 ) + + ch_gt_stats = GT_STAT.out.stats + | map { meta, yml -> yml } + + ch_versions = ch_versions.mix(GT_STAT.out.versions.first()) + + // MODULE: NCBI_FCS_ADAPTOR + ch_fcs_adaptor_inputs = params.ncbi_fcs_adaptor.skip + ? Channel.empty() + : ch_valid_target_assembly + | map { meta, fa -> [ meta.id, fa ] } + + NCBI_FCS_ADAPTOR ( ch_fcs_adaptor_inputs ) + + ch_fcs_adaptor_report = NCBI_FCS_ADAPTOR.out.report + | map { tag, report -> + def is_clean = file(report).readLines().size < 2 + + if (!is_clean) { + log.warn(""" + Adaptor contamination detected in ${tag}. + See the report for further details. + """.stripIndent()) + } + + [ tag, report ] + } + + ch_fcs_adaptor_passed_assembly = params.ncbi_fcs_adaptor.skip + ? ( + ch_valid_target_assembly + | map { meta, fa -> [ meta.id, fa ] } + ) + : ( + ch_fcs_adaptor_report + | map { tag, report -> + [ tag, file(report).readLines().size < 2 ] + } + | filter { tag, is_clean -> is_clean } + | join( + ch_valid_target_assembly + | map { meta, fa -> [ meta.id, fa ] } + ) + | map { tag, clean, fa -> + [ tag, fa ] + } + ) + + ch_versions = ch_versions.mix(NCBI_FCS_ADAPTOR.out.versions.first()) + + // SUBWORKFLOW: NCBI_FCS_GX + ch_fcs_gx_inputs = params.ncbi_fcs_gx.skip + ? Channel.empty() + : ch_valid_target_assembly + | map { meta, fa -> [ meta.id, fa ] } + | combine( Channel.of(file(params.ncbi_fcs_gx.db_path, checkIfExists:true)) ) + + NCBI_FCS_GX( + ch_fcs_gx_inputs.map { tag, fa, db -> [ tag, fa ] }, + ch_fcs_gx_inputs.map { tag, fa, db -> db } ) - | map { - [it[0], it[3]] // [tag, valid fasta path] - } - | set { ch_clean_target_assemblies } - - - // ASSEMBLATHON_STATS - ASSEMBLATHON_STATS(ch_clean_target_assemblies) - | collect - | set { ch_general_stats } - - - // BUSCO - ch_clean_target_assemblies - | combine(Channel.fromList(params.busco.lineage_datasets)) - | map { - [it[0], file(it[1], checkIfExists: true), it[2]] // [tag, assembly fasta path, busco lineage] - } - | BUSCO - - // TIDK - ch_tidk_inputs = params.tidk.skip - ? Channel.empty() - : ch_clean_target_assemblies - | map { tag, fa -> [ [ id: tag ], fa ] } - | combine( - Channel.of(params.tidk.repeat_seq) - ) + + ch_fcs_gx_report = NCBI_FCS_GX.out.gx_report + | map { tag, report -> + def is_clean = file(report).readLines().size < 3 + + if (!is_clean) { + log.warn(""" + Foreign organism contamination detected in ${tag}. + See the report for further details. + """.stripIndent()) + } + + [ tag, report ] + } + + ch_fcs_gx_taxonomy_plot = NCBI_FCS_GX.out.gx_taxonomy_plot + | map { tag, cut, html -> [ tag, html ] } + + ch_fcs_gx_passed_assembly = params.ncbi_fcs_gx.skip + ? ( + ch_valid_target_assembly + | map { meta, fa -> [ meta.id, fa ] } + ) + : ( + ch_fcs_gx_report + | map { tag, report -> + [ tag, file(report).readLines().size < 3 ] + } + | filter { tag, is_clean -> is_clean } + | join( + ch_valid_target_assembly + | map { meta, fa -> [ meta.id, fa ] } + ) + | map { tag, clean, fa -> + [ tag, fa ] + } + ) + + ch_versions = ch_versions.mix(NCBI_FCS_GX.out.versions) + + ch_clean_assembly = ch_fcs_adaptor_passed_assembly + | join( + ch_fcs_gx_passed_assembly + ) + | map { tag, fa, fa2 -> + [ tag, fa ] + } + + // MODULE: ASSEMBLATHON_STATS + ASSEMBLATHON_STATS ( ch_clean_assembly ) + + ch_assemblathon_stats = ASSEMBLATHON_STATS.out.stats + ch_versions = ch_versions.mix(ASSEMBLATHON_STATS.out.versions.first()) + + + // SUBWORKFLOW: BUSCO + ch_busco_inputs = params.busco.skip + ? Channel.empty() + : ch_clean_assembly + | combine(Channel.fromList(params.busco.lineage_datasets)) + | map { tag, fa, lineage -> + [ tag, file(fa, checkIfExists: true), lineage ] + } + BUSCO ( ch_busco_inputs ) + + ch_busco_summary = BUSCO.out.summary + ch_busco_plot = BUSCO.out.plot + ch_versions = ch_versions.mix(BUSCO.out.versions) + + // SUBWORKFLOW: FASTA_EXPLORE_SEARCH_PLOT_TIDK + ch_tidk_inputs = params.tidk.skip + ? Channel.empty() + : ch_clean_assembly + | map { tag, fa -> [ [ id: tag ], fa ] } + | combine( + Channel.of(params.tidk.repeat_seq) + ) FASTA_EXPLORE_SEARCH_PLOT_TIDK( ch_tidk_inputs.map { meta, fa, seq -> [ meta, fa ] }, ch_tidk_inputs.map { meta, fa, seq -> [ meta, seq ] } ) - ch_tidk_outputs = FASTA_EXPLORE_SEARCH_PLOT_TIDK.out.apriori_svg - | mix(FASTA_EXPLORE_SEARCH_PLOT_TIDK.out.aposteriori_svg) - | mix(FASTA_EXPLORE_SEARCH_PLOT_TIDK.out.aposteriori_sequence) - | map { meta, file -> file } - | mix( - Channel.of("$params.tidk.repeat_seq") - | collectFile(name: 'a_priori.sequence', newLine: true) - ) - | collect - - // FASTA_LTRRETRIEVER_LAI - ch_lai_inputs = params.lai.skip - ? Channel.empty() - : ch_clean_target_assemblies - | join( - Channel.fromList(params.lai.monoploid_seqs) - | map { - [it[0], file(it[1], checkIfExists: true)] // [tag, monoploid_seqs] - }, remainder: true - ) - | map { id, fasta, mono -> [ id, fasta, mono ?: [] ] } + ch_tidk_outputs = FASTA_EXPLORE_SEARCH_PLOT_TIDK.out.apriori_svg + | mix(FASTA_EXPLORE_SEARCH_PLOT_TIDK.out.aposteriori_svg) + | mix(FASTA_EXPLORE_SEARCH_PLOT_TIDK.out.aposteriori_sequence) + | map { meta, file -> file } + | mix( + Channel.of("$params.tidk.repeat_seq") + | collectFile(name: 'a_priori.sequence', newLine: true) + ) + + ch_versions = ch_versions.mix(FASTA_EXPLORE_SEARCH_PLOT_TIDK.out.versions) + + // SUBWORKFLOW: FASTA_LTRRETRIEVER_LAI + ch_lai_inputs = params.lai.skip + ? Channel.empty() + : ch_clean_assembly + | join( + Channel.fromList(params.lai.monoploid_seqs) + | map { tag, seqs -> + [ tag, file(seqs, checkIfExists: true)] + }, remainder: true + ) + | map { id, fasta, mono -> [ id, fasta, mono ?: [] ] } FASTA_LTRRETRIEVER_LAI( ch_lai_inputs.map { id, fasta, mono -> [ [ id:id ], fasta ] }, ch_lai_inputs.map { id, fasta, mono -> [ [ id:id ], mono ] }, - false // Not using this flag + false // Not skipping LAI using this flag ) - ch_lai_outputs = FASTA_LTRRETRIEVER_LAI.out.lai_log - | join(FASTA_LTRRETRIEVER_LAI.out.lai_out, remainder: true) - | map { meta, log, out -> out ? [ log, out ] : [log] } - | collect + ch_lai_outputs = FASTA_LTRRETRIEVER_LAI.out.lai_log + | join(FASTA_LTRRETRIEVER_LAI.out.lai_out, remainder: true) + | map { meta, log, out -> out ? [ log, out ] : [log] } + + ch_versions = ch_versions.mix(FASTA_LTRRETRIEVER_LAI.out.versions) - // KRAKEN2 + // SUBWORKFLOW: KRAKEN2 + ch_kraken2_input_assembly = params.kraken2.skip + ? Channel.empty() + : ch_clean_assembly + + ch_kraken2_db_path = params.kraken2.skip + ? Channel.empty() + : Channel.of(file(params.kraken2.db_path, checkIfExists:true)) KRAKEN2( - ch_clean_target_assemblies, - params.kraken2.db_path + ch_kraken2_input_assembly, + ch_kraken2_db_path ) - // HIC_CONTACT_MAP - if(!params.hic.skip) { - if ("${params.hic.paired_reads}".find(/.*[\/].*\.(fastq|fq)\.gz/)) { - ch_paired_reads = Channel.fromFilePairs(params.hic.paired_reads, checkIfExists: true) - } else { - ch_paired_reads = Channel.fromSRA(params.hic.paired_reads) - } - } else { - ch_paired_reads = Channel.empty() - } + ch_kraken2_plot = KRAKEN2.out.plot + ch_versions = ch_versions.mix(KRAKEN2.out.versions) + + // SUBWORKFLOW: FASTQ_TRIM_FASTP_FASTQC + ch_paired_reads = params.hic.skip + ? Channel.empty() + : ( + "${params.hic.paired_reads}".find(/.*[\/].*\.(fastq|fq)\.gz/) + ? Channel.fromFilePairs(params.hic.paired_reads, checkIfExists: true) + : Channel.fromSRA(params.hic.paired_reads) + ) FASTQ_TRIM_FASTP_FASTQC( ch_paired_reads.map { sample, fq -> [ [ id: sample, single_end: false], fq ] }, @@ -174,53 +305,71 @@ workflow ASSEMBLYQC { params.hic.skip_fastp, params.hic.skip_fastqc ) - .reads - | set { ch_cleaned_paired_reads } + + ch_cleaned_paired_reads = FASTQ_TRIM_FASTP_FASTQC.out.reads + ch_versions = ch_versions.mix(FASTQ_TRIM_FASTP_FASTQC.out.versions) + + // SUBWORKFLOW: HIC_CONTACT_MAP + ch_hic_input_assembly = params.hic.skip + ? Channel.empty() + : ch_clean_assembly HIC_CONTACT_MAP( ch_cleaned_paired_reads, - ch_clean_target_assemblies + ch_hic_input_assembly + ) + + ch_hic_map_html = HIC_CONTACT_MAP.out.html + ch_versions = ch_versions.mix(HIC_CONTACT_MAP.out.versions) + + // SUBWORKFLOW: SYNTENY + ch_clean_assembly_seq_list = params.synteny.skip + ? Channel.empty() + : ch_clean_assembly + | join( + Channel.fromList(params.synteny.assembly_seq_list) + | map { tag, seq_list -> + [ tag, file(seq_list, checkIfExists: true) ] + } + ) + + ch_xref_assembly = params.synteny.skip + ? Channel.empty() + : Channel.fromList(params.synteny.xref_assemblies) + | map { tag, xref_fa, xref_seq_list -> + [ tag, file(xref_fa, checkIfExists: true), file(xref_seq_list, checkIfExists: true) ] + } + + SYNTENY( + ch_clean_assembly_seq_list, + ch_xref_assembly + ) + + ch_synteny_plot = SYNTENY.out.plot + ch_versions = ch_versions.mix(SYNTENY.out.versions) + + // MODULE: CUSTOM_DUMPSOFTWAREVERSIONS + CUSTOM_DUMPSOFTWAREVERSIONS( + ch_versions + | unique() + | collectFile(name: 'collated_versions.yml') ) - // SYNTENY - if(!params.synteny.skip) { - ch_clean_target_assemblies - .join( - Channel.fromList(params.synteny.assembly_seq_list) - .map { - [it[0], file(it[1], checkIfExists: true)] // [tag, assembly seq list path] - } - ) - .set { ch_clean_target_assemblies_seq_list } - - Channel.fromList(params.synteny.xref_assemblies) - .map { - [it[0], file(it[1], checkIfExists: true), file(it[2], checkIfExists: true)] // [tag, xref assembly fasta file path, seq list path] - } - .set { ch_with_assemblies } - } else { - Channel.empty() - .set { ch_clean_target_assemblies_seq_list } - - Channel.empty() - .set { ch_with_assemblies } - } - - SYNTENY(ch_clean_target_assemblies_seq_list, ch_with_assemblies) - - // CREATE REPORT + // MODULE: CREATE REPORT CREATE_REPORT( - NCBI_FCS_ADAPTOR.out.reports.ifEmpty([]), - NCBI_FCS_GX.out.fcs_gx_reports.ifEmpty([]), - ch_general_stats.ifEmpty([]), - ch_genometools_gt_stats.ifEmpty([]), - ch_biocode_gff3_stats.ifEmpty([]), - BUSCO.out.list_of_outputs.ifEmpty([]), - ch_tidk_outputs.ifEmpty([]), - ch_lai_outputs.ifEmpty([]), - KRAKEN2.out.list_of_outputs.ifEmpty([]), - HIC_CONTACT_MAP.out.list_of_html_files.ifEmpty([]), - SYNTENY.out.list_of_circos_plots.ifEmpty([]), - Channel.of("$paramsAsJSON") + ch_invalid_assembly_log .map { meta, file -> file }.collect().ifEmpty([]), + ch_invalid_gff3_log .map { meta, file -> file }.collect().ifEmpty([]), + ch_fcs_adaptor_report .map { meta, file -> file }.collect().ifEmpty([]), + ch_fcs_gx_report .mix(ch_fcs_gx_taxonomy_plot).map { meta, file -> file }.collect().ifEmpty([]), + ch_assemblathon_stats .collect().ifEmpty([]), + ch_gt_stats .collect().ifEmpty([]), + ch_busco_summary .mix(ch_busco_plot).collect().ifEmpty([]), + ch_tidk_outputs .collect().ifEmpty([]), + ch_lai_outputs .collect().ifEmpty([]), + ch_kraken2_plot .collect().ifEmpty([]), + ch_hic_map_html .collect().ifEmpty([]), + ch_synteny_plot .collect().ifEmpty([]), + CUSTOM_DUMPSOFTWAREVERSIONS .out.yml, + Channel.of ( jsonifyParams ( params ) ), ) } From f9f6a7da0027308a75b9597ce635874f52a9a5d8 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 19 Feb 2024 20:56:05 +1300 Subject: [PATCH 06/65] Added versions snippet to additional tools --- .../templates/ncbi_fcs_gx/ncbi_fcs_gx.html | 4 ++- conf/modules.config | 2 +- subworkflows/local/kraken2.nf | 7 ++++++ subworkflows/local/ncbi_fcs_gx.nf | 25 +++++++++++++------ 4 files changed, 29 insertions(+), 9 deletions(-) diff --git a/bin/report_modules/templates/ncbi_fcs_gx/ncbi_fcs_gx.html b/bin/report_modules/templates/ncbi_fcs_gx/ncbi_fcs_gx.html index 5d34fd49..e2c2a338 100644 --- a/bin/report_modules/templates/ncbi_fcs_gx/ncbi_fcs_gx.html +++ b/bin/report_modules/templates/ncbi_fcs_gx/ncbi_fcs_gx.html @@ -10,7 +10,9 @@ 10.1101/2023.06.02.543519, GitHub: https://github.com/ncbi/fcs

-

Version: {{ all_stats_dicts['VERSIONS']['SCREEN_SAMPLES']['fcs_gx'] }}

+

+ Version: {{ all_stats_dicts['VERSIONS']['NCBI_FCS_GX_SCREEN_SAMPLES']['fcs_gx'] }} +

DB Version: {{ all_stats_dicts['NCBI_FCS_GX'][0]['report_meta_data'][1]['db']['build-date'] }}

diff --git a/conf/modules.config b/conf/modules.config index 0d1d111b..11aa15b3 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -31,7 +31,7 @@ process { ] } - withName: '.*:NCBI_FCS_GX:SCREEN_SAMPLES' { + withName: NCBI_FCS_GX_SCREEN_SAMPLES { publishDir = [ path: { "${params.outdir}/ncbi_fcs_gx" }, mode: "copy", diff --git a/subworkflows/local/kraken2.nf b/subworkflows/local/kraken2.nf index 9f054dfd..2d3bcb3c 100644 --- a/subworkflows/local/kraken2.nf +++ b/subworkflows/local/kraken2.nf @@ -34,6 +34,7 @@ workflow KRAKEN2 { ch_versions = Channel.empty() | mix(RUN_KRAKEN2.out.versions.first()) | mix(UNTAR.out.versions.first()) + | mix(KRONA_PLOT.out.versions.first()) emit: plot = KRONA_PLOT.out.plot @@ -86,10 +87,16 @@ process KRONA_PLOT { output: tuple path("*.kraken2.krona.cut"), path("*.kraken2.krona.html"), emit: plot + path "versions.yml" , emit: versions script: """ perl -lane '@a=split /\\t/; if (\$a[2] =~ /taxid\\s+(\\d+)/) {print "\$a[1]\\t\$1\\t1\\t\$a[3]";}' $kraken2_cut > "${hap_name}.kraken2.krona.cut" ktImportTaxonomy -i -o "${hap_name}.kraken2.krona.html" -m "4" "${hap_name}.kraken2.krona.cut" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + KronaTools: \$(ktImportTaxonomy | sed -n '/KronaTools/s/KronaTools//p' | tr -d ' _/[:space:]' | sed 's/-ktImportTaxonomy\\\\//1') + END_VERSIONS """ } diff --git a/subworkflows/local/ncbi_fcs_gx.nf b/subworkflows/local/ncbi_fcs_gx.nf index fc3832a1..e114968f 100644 --- a/subworkflows/local/ncbi_fcs_gx.nf +++ b/subworkflows/local/ncbi_fcs_gx.nf @@ -10,10 +10,10 @@ workflow NCBI_FCS_GX { ch_all_samples = SETUP_SAMPLE.out.fsata | collect - // MODULE: SCREEN_SAMPLES - SCREEN_SAMPLES ( ch_all_samples, db_path ) + // MODULE: NCBI_FCS_GX_SCREEN_SAMPLES + NCBI_FCS_GX_SCREEN_SAMPLES ( ch_all_samples, db_path ) - ch_gx_report = SCREEN_SAMPLES.out.fcs_gx_reports + ch_gx_report = NCBI_FCS_GX_SCREEN_SAMPLES.out.fcs_gx_reports | flatten | map { def parts = it.getName().split("\\.") @@ -21,7 +21,7 @@ workflow NCBI_FCS_GX { [tag, it] } - ch_gx_taxonomy = SCREEN_SAMPLES.out.fcs_gx_taxonomies + ch_gx_taxonomy = NCBI_FCS_GX_SCREEN_SAMPLES.out.fcs_gx_taxonomies | flatten | map { def parts = it.getName().split("\\.") @@ -33,11 +33,16 @@ workflow NCBI_FCS_GX { FCS_GX_KRONA_PLOT ( ch_gx_taxonomy ) ch_gx_taxonomy_plot = FCS_GX_KRONA_PLOT.out.plot + + ch_versions = Channel.empty() + | mix(NCBI_FCS_GX_SCREEN_SAMPLES.out.versions.first()) + | mix(FCS_GX_KRONA_PLOT.out.versions.first()) + emit: gx_report = ch_gx_report gx_taxonomy = ch_gx_taxonomy gx_taxonomy_plot = ch_gx_taxonomy_plot - versions = Channel.empty().mix(SCREEN_SAMPLES.out.versions.first()) + versions = ch_versions } process SETUP_SAMPLE { @@ -61,7 +66,7 @@ process SETUP_SAMPLE { } -process SCREEN_SAMPLES { +process NCBI_FCS_GX_SCREEN_SAMPLES { tag "all samples" label "process_high" label "process_long" @@ -110,7 +115,8 @@ process FCS_GX_KRONA_PLOT { tuple val(tag_name), path(fcs_gx_taxonomy) output: - tuple path("${tag_name}.inter.tax.rpt.tsv"), path("${tag_name}.fcs.gx.krona.cut"), path("${tag_name}.fcs.gx.krona.html"), emit: plot + tuple path("${tag_name}.inter.tax.rpt.tsv"), path("${tag_name}.fcs.gx.krona.cut"), path("${tag_name}.fcs.gx.krona.html") , emit: plot + path "versions.yml" , emit: versions script: """ @@ -127,5 +133,10 @@ process FCS_GX_KRONA_PLOT { >> "${tag_name}.fcs.gx.krona.cut" ktImportTaxonomy -i -o "${tag_name}.fcs.gx.krona.html" -m "4" "${tag_name}.fcs.gx.krona.cut" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + KronaTools: \$(ktImportTaxonomy | sed -n '/KronaTools/s/KronaTools//p' | tr -d ' _/[:space:]' | sed 's/-ktImportTaxonomy\\\\//1') + END_VERSIONS """ } From 24151a6210bb11654c6465d5fa344654d1fca7b7 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 20 Feb 2024 09:29:48 +1300 Subject: [PATCH 07/65] Added nf-core/TEMPLATE --- .devcontainer/devcontainer.json | 28 + .editorconfig | 16 +- .gitattributes | 4 + .github/.dockstore.yml | 6 + 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diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json new file mode 100644 index 00000000..4ecfbfe3 --- /dev/null +++ b/.devcontainer/devcontainer.json @@ -0,0 +1,28 @@ +{ + "name": "nfcore", + "image": "nfcore/gitpod:latest", + "remoteUser": "gitpod", + "runArgs": ["--privileged"], + + // Configure tool-specific properties. + "customizations": { + // Configure properties specific to VS Code. + "vscode": { + // Set *default* container specific settings.json values on container create. + "settings": { + "python.defaultInterpreterPath": "/opt/conda/bin/python", + "python.linting.enabled": true, + "python.linting.pylintEnabled": true, + "python.formatting.autopep8Path": "/opt/conda/bin/autopep8", + "python.formatting.yapfPath": "/opt/conda/bin/yapf", + "python.linting.flake8Path": "/opt/conda/bin/flake8", + "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", + "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", + "python.linting.pylintPath": "/opt/conda/bin/pylint" + }, + + // Add the IDs of extensions you want installed when the container is created. + "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"] + } + } +} diff --git a/.editorconfig b/.editorconfig index e572f526..9b990088 100644 --- a/.editorconfig +++ b/.editorconfig @@ -8,10 +8,11 @@ trim_trailing_whitespace = true indent_size = 4 indent_style = space -[*.{md,yml,yaml,cff}] +[*.{md,yml,yaml,html,css,scss,js}] indent_size = 2 -[{LICENSE,LEGAL.txt}] +# These files are edited and tested upstream in nf-core/modules +[/modules/nf-core/**] charset = unset end_of_line = unset insert_final_newline = unset @@ -19,8 +20,13 @@ trim_trailing_whitespace = unset indent_style = unset indent_size = unset -[*.{pl,pm}] +[/assets/email*] indent_size = unset -[*.nf.test] -insert_final_newline = false +# ignore Readme +[README.md] +indent_style = unset + +# ignore python +[*.{py}] +indent_style = unset diff --git a/.gitattributes b/.gitattributes new file mode 100644 index 00000000..7a2dabc2 --- /dev/null +++ b/.gitattributes @@ -0,0 +1,4 @@ +*.config linguist-language=nextflow +*.nf.test linguist-language=nextflow +modules/nf-core/** linguist-generated +subworkflows/nf-core/** linguist-generated diff --git a/.github/.dockstore.yml b/.github/.dockstore.yml new file mode 100644 index 00000000..191fabd2 --- /dev/null +++ b/.github/.dockstore.yml @@ -0,0 +1,6 @@ +# Dockstore config version, not pipeline version +version: 1.2 +workflows: + - subclass: nfl + primaryDescriptorPath: /nextflow.config + publish: True diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md new file mode 100644 index 00000000..ab01b3d5 --- /dev/null +++ b/.github/CONTRIBUTING.md @@ -0,0 +1,115 @@ +# plant-food-research-open/assemblyqc: Contributing Guidelines + +Hi there! +Many thanks for taking an interest in improving plant-food-research-open/assemblyqc. + +We try to manage the required tasks for plant-food-research-open/assemblyqc using GitHub issues, you probably came to this page when creating one. +Please use the pre-filled template to save time. + +However, don't be put off by this template - other more general issues and suggestions are welcome! +Contributions to the code are even more welcome ;) + +## Contribution workflow + +If you'd like to write some code for plant-food-research-open/assemblyqc, the standard workflow is as follows: + +1. Check that there isn't already an issue about your idea in the [plant-food-research-open/assemblyqc issues](https://github.com/plant-food-research-open/assemblyqc/issues) to avoid duplicating work. If there isn't one already, please create one so that others know you're working on this +2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [plant-food-research-open/assemblyqc repository](https://github.com/plant-food-research-open/assemblyqc) to your GitHub account +3. Make the necessary changes / additions within your forked repository following [Pipeline conventions](#pipeline-contribution-conventions) +4. Use `nf-core schema build` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10). +5. Submit a Pull Request against the `dev` branch and wait for the code to be reviewed and merged + +If you're not used to this workflow with git, you can start with some [docs from GitHub](https://help.github.com/en/github/collaborating-with-issues-and-pull-requests) or even their [excellent `git` resources](https://try.github.io/). + +## Tests + +You can optionally test your changes by running the pipeline locally. Then it is recommended to use the `debug` profile to +receive warnings about process selectors and other debug info. Example: `nextflow run . -profile debug,test,docker --outdir `. + +When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests. +Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then. + +There are typically two types of tests that run: + +### Lint tests + +`nf-core` has a [set of guidelines](https://nf-co.re/developers/guidelines) which all pipelines must adhere to. +To enforce these and ensure that all pipelines stay in sync, we have developed a helper tool which runs checks on the pipeline code. This is in the [nf-core/tools repository](https://github.com/nf-core/tools) and once installed can be run locally with the `nf-core lint ` command. + +If any failures or warnings are encountered, please follow the listed URL for more documentation. + +### Pipeline tests + +Each `nf-core` pipeline should be set up with a minimal set of test-data. +`GitHub Actions` then runs the pipeline on this data to ensure that it exits successfully. +If there are any failures then the automated tests fail. +These tests are run both with the latest available version of `Nextflow` and also the minimum required version that is stated in the pipeline code. + +## Patch + +:warning: Only in the unlikely and regretful event of a release happening with a bug. + +- On your own fork, make a new branch `patch` based on `upstream/master`. +- Fix the bug, and bump version (X.Y.Z+1). +- A PR should be made on `master` from patch to directly this particular bug. + +## Pipeline contribution conventions + +To make the plant-food-research-open/assemblyqc code and processing logic more understandable for new contributors and to ensure quality, we semi-standardise the way the code and other contributions are written. + +### Adding a new step + +If you wish to contribute a new step, please use the following coding standards: + +1. Define the corresponding input channel into your new process from the expected previous process channel +2. Write the process block (see below). +3. Define the output channel if needed (see below). +4. Add any new parameters to `nextflow.config` with a default (see below). +5. Add any new parameters to `nextflow_schema.json` with help text (via the `nf-core schema build` tool). +6. Add sanity checks and validation for all relevant parameters. +7. Perform local tests to validate that the new code works as expected. +8. If applicable, add a new test command in `.github/workflow/ci.yml`. +9. Update MultiQC config `assets/multiqc_config.yml` so relevant suffixes, file name clean up and module plots are in the appropriate order. If applicable, add a [MultiQC](https://https://multiqc.info/) module. +10. Add a description of the output files and if relevant any appropriate images from the MultiQC report to `docs/output.md`. + +### Default values + +Parameters should be initialised / defined with default values in `nextflow.config` under the `params` scope. + +Once there, use `nf-core schema build` to add to `nextflow_schema.json`. + +### Default processes resource requirements + +Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. + +The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block. + +### Naming schemes + +Please use the following naming schemes, to make it easy to understand what is going where. + +- initial process channel: `ch_output_from_` +- intermediate and terminal channels: `ch__for_` + +### Nextflow version bumping + +If you are using a new feature from core Nextflow, you may bump the minimum required version of nextflow in the pipeline with: `nf-core bump-version --nextflow . [min-nf-version]` + +### Images and figures + +For overview images and other documents we follow the nf-core [style guidelines and examples](https://nf-co.re/developers/design_guidelines). + +## GitHub Codespaces + +This repo includes a devcontainer configuration which will create a GitHub Codespaces for Nextflow development! This is an online developer environment that runs in your browser, complete with VSCode and a terminal. + +To get started: + +- Open the repo in [Codespaces](https://github.com/plant-food-research-open/assemblyqc/codespaces) +- Tools installed + - nf-core + - Nextflow + +Devcontainer specs: + +- [DevContainer config](.devcontainer/devcontainer.json) diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml new file mode 100644 index 00000000..c22d7e64 --- /dev/null +++ b/.github/ISSUE_TEMPLATE/bug_report.yml @@ -0,0 +1,54 @@ +name: Bug report +description: Report something that is broken or incorrect +labels: bug +body: +- type: textarea + id: description + attributes: + label: Description of the bug + description: A clear and concise description of what the bug is. + validations: + required: true +- type: textarea + id: command_used + attributes: + label: Command used and terminal output + description: Steps to reproduce the behaviour. Please paste the command you used + to launch the pipeline and the output from your terminal. + render: console + placeholder: '$ nextflow run ... + + + Some output where something broke + + ' +- type: textarea + id: files + attributes: + label: Relevant files + description: 'Please drag and drop the relevant files here. Create a `.zip` archive + if the extension is not allowed. + + Your verbose log file `.nextflow.log` is often useful _(this is a hidden file + in the directory where you launched the pipeline)_ as well as custom Nextflow + configuration files. + + ' +- type: textarea + id: system + attributes: + label: System information + description: '* Nextflow version _(eg. 23.04.0)_ + + * Hardware _(eg. HPC, Desktop, Cloud)_ + + * Executor _(eg. slurm, local, awsbatch)_ + + * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter, Charliecloud, + or Apptainer)_ + + * OS _(eg. CentOS Linux, macOS, Linux Mint)_ + + * Version of plant-food-research-open/assemblyqc _(eg. 1.1, 1.5, 1.8.2)_ + + ' diff --git a/.github/ISSUE_TEMPLATE/feature_request.yml b/.github/ISSUE_TEMPLATE/feature_request.yml new file mode 100644 index 00000000..6287e290 --- /dev/null +++ b/.github/ISSUE_TEMPLATE/feature_request.yml @@ -0,0 +1,11 @@ +name: Feature request +description: Suggest an idea for the plant-food-research-open/assemblyqc pipeline +labels: enhancement +body: + - type: textarea + id: description + attributes: + label: Description of feature + description: Please describe your suggestion for a new feature. It might help to describe a problem or use case, plus any alternatives that you have considered. + validations: + required: true diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md new file mode 100644 index 00000000..3da295ca --- /dev/null +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -0,0 +1,25 @@ + + +## PR checklist + +- [ ] This comment contains a description of changes (with reason). +- [ ] If you've fixed a bug or added code that should be tested, add tests! +- [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/plant-food-research-open/assemblyqc/tree/master/.github/CONTRIBUTING.md) +- [ ] Make sure your code lints (`nf-core lint`). +- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). +- [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). +- [ ] Usage Documentation in `docs/usage.md` is updated. +- [ ] Output Documentation in `docs/output.md` is updated. +- [ ] `CHANGELOG.md` is updated. +- [ ] `README.md` is updated (including new tool citations and authors/contributors). diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml new file mode 100644 index 00000000..752ea1bf --- /dev/null +++ b/.github/workflows/branch.yml @@ -0,0 +1,44 @@ +name: nf-core branch protection +# This workflow is triggered on PRs to master branch on the repository +# It fails when someone tries to make a PR against the nf-core `master` branch instead of `dev` +on: + pull_request_target: + branches: [master] + +jobs: + test: + runs-on: ubuntu-latest + steps: + # PRs to the nf-core repo master branch are only ok if coming from the nf-core repo `dev` or any `patch` branches + - name: Check PRs + if: github.repository == 'plant-food-research-open/assemblyqc' + run: | + { [[ ${{github.event.pull_request.head.repo.full_name }} == plant-food-research-open/assemblyqc ]] && [[ $GITHUB_HEAD_REF == "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]] + + # If the above check failed, post a comment on the PR explaining the failure + # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets + - name: Post PR comment + if: failure() + uses: mshick/add-pr-comment@v2 + with: + message: | + ## This PR is against the `master` branch :x: + + * Do not close this PR + * Click _Edit_ and change the `base` to `dev` + * This CI test will remain failed until you push a new commit + + --- + + Hi @${{ github.event.pull_request.user.login }}, + + It looks like this pull-request is has been made against the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `master` branch. + The `master` branch on nf-core repositories should always contain code from the latest release. + Because of this, PRs to `master` are only allowed if they come from the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `dev` branch. + + You do not need to close this PR, you can change the target branch to `dev` by clicking the _"Edit"_ button at the top of this page. + Note that even after this, the test will continue to show as failing until you push a new commit. + + Thanks again for your contribution! + repo-token: ${{ secrets.GITHUB_TOKEN }} + allow-repeats: false diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml new file mode 100644 index 00000000..9e9668cc --- /dev/null +++ b/.github/workflows/ci.yml @@ -0,0 +1,43 @@ +name: nf-core CI +# This workflow runs the pipeline with the minimal test dataset to check that it completes without any syntax errors +on: + push: + branches: + - dev + pull_request: + release: + types: [published] + +env: + NXF_ANSI_LOG: false + +concurrency: + group: "${{ github.workflow }}-${{ github.event.pull_request.number || github.ref }}" + cancel-in-progress: true + +jobs: + test: + name: Run pipeline with test data + # Only run on push if this is the nf-core dev branch (merged PRs) + if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'plant-food-research-open/assemblyqc') }}" + runs-on: ubuntu-latest + strategy: + matrix: + NXF_VER: + - "23.04.0" + - "latest-everything" + steps: + - name: Check out pipeline code + uses: actions/checkout@v4 + + - name: Install Nextflow + uses: nf-core/setup-nextflow@v1 + with: + version: "${{ matrix.NXF_VER }}" + + - name: Run pipeline with test data + # TODO nf-core: You can customise CI pipeline run tests as required + # For example: adding multiple test runs with different parameters + # Remember that you can parallelise this by using strategy.matrix + run: | + nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml new file mode 100644 index 00000000..e37cfda5 --- /dev/null +++ b/.github/workflows/clean-up.yml @@ -0,0 +1,24 @@ +name: "Close user-tagged issues and PRs" +on: + schedule: + - cron: "0 0 * * 0" # Once a week + +jobs: + clean-up: + runs-on: ubuntu-latest + permissions: + issues: write + pull-requests: write + steps: + - uses: actions/stale@v9 + with: + stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days." + stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful." + close-issue-message: "This issue was closed because it has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor and then staled for 20 days with no activity." + days-before-stale: 30 + days-before-close: 20 + days-before-pr-close: -1 + any-of-labels: "awaiting-changes,awaiting-feedback" + exempt-issue-labels: "WIP" + exempt-pr-labels: "WIP" + repo-token: "${{ secrets.GITHUB_TOKEN }}" diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml new file mode 100644 index 00000000..47168689 --- /dev/null +++ b/.github/workflows/download_pipeline.yml @@ -0,0 +1,67 @@ +name: Test successful pipeline download with 'nf-core download' + +# Run the workflow when: +# - dispatched manually +# - when a PR is opened or reopened to master branch +# - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev. +on: + workflow_dispatch: + pull_request: + types: + - opened + branches: + - master + pull_request_target: + branches: + - master + +env: + NXF_ANSI_LOG: false + +jobs: + download: + runs-on: ["self-hosted"] + steps: + - name: Install Nextflow + uses: nf-core/setup-nextflow@v1 + + - uses: actions/setup-python@v5 + with: + python-version: "3.11" + architecture: "x64" + - uses: eWaterCycle/setup-singularity@v7 + with: + singularity-version: 3.8.3 + + - name: Install dependencies + run: | + python -m pip install --upgrade pip + pip install git+https://github.com/nf-core/tools.git@dev + + - name: Get the repository name and current branch set as environment variable + run: | + echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV} + echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} + echo "REPO_BRANCH=${GITHUB_REF#refs/heads/}" >> ${GITHUB_ENV} + + - name: Download the pipeline + env: + NXF_SINGULARITY_CACHEDIR: ./ + run: | + nf-core download ${{ env.REPO_LOWERCASE }} \ + --revision ${{ env.REPO_BRANCH }} \ + --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ + --compress "none" \ + --container-system 'singularity' \ + --container-library "quay.io" -l "docker.io" -l "ghcr.io" \ + --container-cache-utilisation 'amend' \ + --download-configuration + + - name: Inspect download + run: tree ./${{ env.REPOTITLE_LOWERCASE }} + + - name: Run the downloaded pipeline + env: + NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_HOME_MOUNT: true + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml new file mode 100644 index 00000000..41963bec --- /dev/null +++ b/.github/workflows/fix-linting.yml @@ -0,0 +1,48 @@ +name: Fix linting from a comment +on: + issue_comment: + types: [created] + +jobs: + deploy: + # Only run if comment is on a PR with the main repo, and if it contains the magic keywords + if: > + contains(github.event.comment.html_url, '/pull/') && + contains(github.event.comment.body, '@nf-core-bot fix linting') && + github.repository == 'plant-food-research-open/assemblyqc' + runs-on: ubuntu-latest + steps: + # Use the @nf-core-bot token to check out so we can push later + - uses: actions/checkout@v4 + with: + token: ${{ secrets.nf_core_bot_auth_token }} + + # Action runs on the issue comment, so we don't get the PR by default + # Use the gh cli to check out the PR + - name: Checkout Pull Request + run: gh pr checkout ${{ github.event.issue.number }} + env: + GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }} + + - name: Set up Python 3.11 + uses: actions/setup-python@v5 + with: + python-version: 3.11 + cache: "pip" + + - name: Install pre-commit + run: pip install pre-commit + + - name: Run pre-commit + run: pre-commit run --all-files || echo "status=fail" >> $GITHUB_ENV + + - name: Commit & push changes + if: env.status == 'fail' + run: | + git config user.email "core@nf-co.re" + git config user.name "nf-core-bot" + git config push.default upstream + git add . + git status + git commit -m "[automated] Fix linting with pre-commit" + git push diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml new file mode 100644 index 00000000..81cd098e --- /dev/null +++ b/.github/workflows/linting.yml @@ -0,0 +1,69 @@ +name: nf-core linting +# This workflow is triggered on pushes and PRs to the repository. +# It runs the `nf-core lint` and markdown lint tests to ensure +# that the code meets the nf-core guidelines. +on: + push: + branches: + - dev + pull_request: + release: + types: [published] + +jobs: + pre-commit: + runs-on: ubuntu-latest + steps: + - uses: actions/checkout@v4 + + - name: Set up Python 3.11 + uses: actions/setup-python@v5 + with: + python-version: 3.11 + cache: "pip" + + - name: Install pre-commit + run: pip install pre-commit + + - name: Run pre-commit + run: pre-commit run --all-files + + nf-core: + runs-on: ubuntu-latest + steps: + - name: Check out pipeline code + uses: actions/checkout@v4 + + - name: Install Nextflow + uses: nf-core/setup-nextflow@v1 + + - uses: actions/setup-python@v5 + with: + python-version: "3.11" + architecture: "x64" + + - name: Install dependencies + run: | + python -m pip install --upgrade pip + pip install nf-core + + - name: Run nf-core lint + env: + GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }} + GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} + GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }} + run: nf-core -l lint_log.txt lint --dir ${GITHUB_WORKSPACE} --markdown lint_results.md + + - name: Save PR number + if: ${{ always() }} + run: echo ${{ github.event.pull_request.number }} > PR_number.txt + + - name: Upload linting log file artifact + if: ${{ always() }} + uses: actions/upload-artifact@v4 + with: + name: linting-logs + path: | + lint_log.txt + lint_results.md + PR_number.txt diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml new file mode 100644 index 00000000..147bcd10 --- /dev/null +++ b/.github/workflows/linting_comment.yml @@ -0,0 +1,28 @@ +name: nf-core linting comment +# This workflow is triggered after the linting action is complete +# It posts an automated comment to the PR, even if the PR is coming from a fork + +on: + workflow_run: + workflows: ["nf-core linting"] + +jobs: + test: + runs-on: ubuntu-latest + steps: + - name: Download lint results + uses: dawidd6/action-download-artifact@v3 + with: + workflow: linting.yml + workflow_conclusion: completed + + - name: Get PR number + id: pr_number + run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT + + - name: Post PR comment + uses: marocchino/sticky-pull-request-comment@v2 + with: + GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} + number: ${{ steps.pr_number.outputs.pr_number }} + path: linting-logs/lint_results.md diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml new file mode 100644 index 00000000..21ac3f06 --- /dev/null +++ b/.github/workflows/release-announcements.yml @@ -0,0 +1,68 @@ +name: release-announcements +# Automatic release toot and tweet anouncements +on: + release: + types: [published] + workflow_dispatch: + +jobs: + toot: + runs-on: ubuntu-latest + steps: + - uses: rzr/fediverse-action@master + with: + access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }} + host: "mstdn.science" # custom host if not "mastodon.social" (default) + # GitHub event payload + # https://docs.github.com/en/developers/webhooks-and-events/webhooks/webhook-events-and-payloads#release + message: | + Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! + + Please see the changelog: ${{ github.event.release.html_url }} + + send-tweet: + runs-on: ubuntu-latest + + steps: + - uses: actions/setup-python@v5 + with: + python-version: "3.10" + - name: Install dependencies + run: pip install tweepy==4.14.0 + - name: Send tweet + shell: python + run: | + import os + import tweepy + + client = tweepy.Client( + access_token=os.getenv("TWITTER_ACCESS_TOKEN"), + access_token_secret=os.getenv("TWITTER_ACCESS_TOKEN_SECRET"), + consumer_key=os.getenv("TWITTER_CONSUMER_KEY"), + consumer_secret=os.getenv("TWITTER_CONSUMER_SECRET"), + ) + tweet = os.getenv("TWEET") + client.create_tweet(text=tweet) + env: + TWEET: | + Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! + + Please see the changelog: ${{ github.event.release.html_url }} + TWITTER_CONSUMER_KEY: ${{ secrets.TWITTER_CONSUMER_KEY }} + TWITTER_CONSUMER_SECRET: ${{ secrets.TWITTER_CONSUMER_SECRET }} + TWITTER_ACCESS_TOKEN: ${{ secrets.TWITTER_ACCESS_TOKEN }} + TWITTER_ACCESS_TOKEN_SECRET: ${{ secrets.TWITTER_ACCESS_TOKEN_SECRET }} + + bsky-post: + runs-on: ubuntu-latest + steps: + - uses: zentered/bluesky-post-action@v0.1.0 + with: + post: | + Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! + + Please see the changelog: ${{ github.event.release.html_url }} + env: + BSKY_IDENTIFIER: ${{ secrets.BSKY_IDENTIFIER }} + BSKY_PASSWORD: ${{ secrets.BSKY_PASSWORD }} + # diff --git a/.gitignore b/.gitignore index fdbb53de..5124c9ac 100644 --- a/.gitignore +++ b/.gitignore @@ -1,9 +1,8 @@ -.DS_Store -*.pyc -__pycahce__ .nextflow* work/ -results/ -*.stdout -*.stderr data/ +results/ +.DS_Store +testing/ +testing* +*.pyc diff --git a/.gitpod.yml b/.gitpod.yml new file mode 100644 index 00000000..363d5b1d --- /dev/null +++ b/.gitpod.yml @@ -0,0 +1,22 @@ +image: nfcore/gitpod:latest +tasks: + - name: Update Nextflow and setup pre-commit + command: | + pre-commit install --install-hooks + nextflow self-update + - name: unset JAVA_TOOL_OPTIONS + command: | + unset JAVA_TOOL_OPTIONS + +vscode: + extensions: # based on nf-core.nf-core-extensionpack + - codezombiech.gitignore # Language support for .gitignore files + # - cssho.vscode-svgviewer # SVG viewer + - esbenp.prettier-vscode # Markdown/CommonMark linting and style checking for Visual Studio Code + - eamodio.gitlens # Quickly glimpse into whom, why, and when a line or code block was changed + - EditorConfig.EditorConfig # override user/workspace settings with settings found in .editorconfig files + - Gruntfuggly.todo-tree # Display TODO and FIXME in a tree view in the activity bar + - mechatroner.rainbow-csv # Highlight columns in csv files in different colors + # - nextflow.nextflow # Nextflow syntax highlighting + - oderwat.indent-rainbow # Highlight indentation level + - streetsidesoftware.code-spell-checker # Spelling checker for source code diff --git a/.nf-core.yml b/.nf-core.yml index 3805dc81..98319d1b 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1 +1,24 @@ +lint: + files_exist: + - CODE_OF_CONDUCT.md + - assets/nf-core-assemblyqc_logo_light.png + - docs/images/nf-core-assemblyqc_logo_light.png + - docs/images/nf-core-assemblyqc_logo_dark.png + - .github/ISSUE_TEMPLATE/config.yml + - .github/workflows/awstest.yml + - .github/workflows/awsfulltest.yml + files_unchanged: + - CODE_OF_CONDUCT.md + - assets/nf-core-assemblyqc_logo_light.png + - docs/images/nf-core-assemblyqc_logo_light.png + - docs/images/nf-core-assemblyqc_logo_dark.png + - .github/ISSUE_TEMPLATE/bug_report.yml + multiqc_config: + - report_comment + nextflow_config: + - manifest.name + - manifest.homePage repository_type: pipeline +template: + prefix: plant-food-research-open + skip: [] diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index 94efb370..984321ff 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -1,24 +1,10 @@ repos: - repo: https://github.com/pre-commit/mirrors-prettier - rev: "v3.1.0" + rev: "v3.1.1" hooks: - id: prettier - - repo: https://github.com/psf/black - rev: 22.10.0 - hooks: - - id: black - repo: https://github.com/editorconfig-checker/editorconfig-checker.python - rev: 2.7.3 + rev: "2.7.3" hooks: - id: editorconfig-checker alias: ec - - repo: local - hooks: - - id: version_checks - name: Version checks - language: system - entry: > - ./version_check.sh - always_run: true - fail_fast: true - pass_filenames: false diff --git a/.prettierignore b/.prettierignore index e5fc455d..437d763d 100644 --- a/.prettierignore +++ b/.prettierignore @@ -1,9 +1,12 @@ -# gitignore -.DS_Store -*.pyc -__pycahce__ +email_template.html +adaptivecard.json +slackreport.json .nextflow* work/ +data/ results/ -*.stdout -*.stderr +.DS_Store +testing/ +testing* +*.pyc +bin/ diff --git a/CHANGELOG.md b/CHANGELOG.md index 03dd8732..4aed6c94 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -1,289 +1,16 @@ -# Change Log +# plant-food-research-open/assemblyqc: Changelog -## Version 1.4 (20-Feb-2023) +The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) +and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -1. Now it is possible to skip FASTP and FASTQC for the HIC module -2. Renamed ASSEMBLY_QC workflow to ASSEMBLYQC -3. Now using nf-core/FASTA_EXPLORE_SEARCH_PLOT_TIDK -4. Now redirecting validation errors to AssemblyQC report -5. Simplified layout of CITATIONS.md file -6. Now using pfr/gff3_validate sub-workflow for gff3 validation -7. Now listing software versions from the versions.yml file -8. Replaced custom GUNZIP module with nf-core/gunzip -9. Replaced custom gt/stat with pfr/gt/stat -10. Replaced custom fasta_validator with nf-core/fastavalidator -11. Removed BIOCODE GFF3 STATS owing to its frequent failures -12. Added pre-commit version checking -13. Now gt/stat reports extended stats and multiple distribution plots have been added to the report -14. Added a tools tab to the report which lists the tools used by the pipeline to create the report -15. Refactored and cleaned data flows for all the custom sub-workflow +## v1.4dev - [date] -## Version 1.3 (08-Feb-2023) +Initial release of plant-food-research-open/assemblyqc, created with the [nf-core](https://nf-co.re/) template. -1. Docker engine is now also supported -2. Added Amazon Genomics CLI project file and a minimal test params file: [./docs/test_params/test_agc.json](./docs/test_params/test_agc.json) -3. Downgraded to Nextflow 22.04.3 -4. Removed container setup process from NCBI_FCS_ADAPTOR workflow -5. The pipeline does not download the kraken database anymore -6. Fixed a bug in SYNTENY/DNADIFF module which caused failure on AWS Batch -7. Now tar zipped database can be directly used with Kraken2 -8. Removed `db_manifest_url` parameter for the NCBI_FCS_GX workflow -9. Now using parallel version of LTRHARVEST from the EDTA package -10. BWA_INDEX_AND_MEM can now run for two days -11. Now using FASTQ_BWA_MEM_SAMBLASTER subworkflow to optimize SAM file transfer on AWS +### `Added` -## Version 1.2 (18-Dec-2023) +### `Fixed` -1. Switched to apptainer from singularity -2. Now requiring Nextflow/23.04.4 -3. Simplified output directory from `outdir.main` to `outdir` -4. Changed profile name from slurm to pfr -5. Now using APPTAINER_BINDPATH to provide TMPDIR -6. Integrated and tested FASTA_LTRRETRIEVER_LAI to replace EDTA_LAI sub-workflow -7. Corrected LAI version to beta3.2 +### `Dependencies` -### FASTA_LTRRETRIEVER_LAI vs EDTA_LAI - -For a ~600 MB assembly, EDTA (without sensitive flag) takes ~25 hours of compute time. Whereas, FASTA_LTRRETRIEVER_LAI sub-workflow ( LTRHARVEST+LTRFINDER -> LTRRETRIEVER ) takes ~2.5 hours of compute time. LAI estimates for four plant assemblies are listed below. - -| Assembly | EDTA_LAI | FASTA_LTRRETRIEVER_LAI | -| ----------- | -------- | ---------------------- | -| ck6901m/v2 | 18.43 | 16.19 | -| donghong/v1 | 19.03 | 16.85 | -| red5/v2.1 | 18.75 | 16.59 | -| tair/v10 | 18.06 | 17.42 | - -## Version 1.1 (09-Nov-2023) - -1. Now running kraken2 with a single cpu. -2. Now pulling containers from https://depot.galaxyproject.org/singularity/ - -## Version 1.0.1 (07-Sep-2023) - -1. Now pipeline timeline, report, and trace are enabled by default. -2. Included `procps` package where needed to allow NextFlow to collect system statistics. - -## Version 1 (25-Jul-2023) - -Same as Version 1 RC6c - -## Version 1 (RC6c; 20-Jul-2023) - -1. Added logic for the `-mono` parameter in LAI. This parameter allows correct LAI calculation for polyploid assemblies. -2. Fixed the typo in `assemblathon_stats` in nextflow.config. -3. Fixed the test_full.config example config and docs to exclude the mitochondrion genome from synteny and LAI modules. -4. Now saving `*.EDTA.TEanno.gff3` and `*.EDTA.intact.gff3` with original fasta ids. -5. Removed comments from the ID lines of the FASTA file before running lAI. -6. Now presenting the PARAMS page as formatted JSON rather than a table. -7. Now SAMBLASTER can run up to 20 hours. -8. (RC6b) NCBI FCS GX taxonomy is now presented as a Krona plot. (RC6c) No hits are included. Sequence length is used when calculating abundance. -9. (RC6c) Krona plot for Kraken2 now uses sequence length for abundance calculation. -10. Made ASSEMBLATHON_STATS robust to missing paths declared in the PATH variable. - -## Version 1 (RC5; 22-Jun-2023) - -1. Updated README in accordance with SPO Editor. -2. Added a note on LTR sequence identity in the nextflow.config. -3. Split MATLOCK_BAM2_JUICER module into MATLOCK_BAM2_JUICER and JUICER_SORT and using `--parallel` with `sort`. - -## Version 1 (RC4; 15-Jun-2023) - -1. Fixed a bug in the BIOCODE GFF3 STATS module which resulted in a cramped up plot of CDS vs mRNA counts. - -## Version 1 (RC3; 14-Jun-2023) - -1. Fixed a bug in the BIOCODE GFF3 STATS module which prevented it from processing valid gff3 files. - -## Version 1 (RC2; 13-Jun-2023) - -1. Added labels to the pipeline flowchart. -2. Update the README based on team feedback. - -## Version 1 (RC1; 12-Jun-2023) - -1. Added validation for fasta and gff3 files. -2. Added support for compressed files (fasta.gz, gff3.gz). -3. Added BIOCODE GFF3 STATS. -4. Added correspondence checks between gff3 and fasta files. -5. Now using standard mode as default for LAI. -6. Added information regarding LAI:EDTA time requirements for various genome sizes. -7. Added information regarding influence of LAI:EDTA:is_sensitive flag on LAI scores. -8. Added a params summary page. -9. Now the default config file (nextflow.config) is designed to run out-of-the-box at PFR. There is no need to do any setup. -10. "report" is now the default results folder. -11. Added documentation and configuration files for examples based on publicly accessible data from NCBI. -12. Added test configurations for Fungal, Bacterial, and Viral assemblies. -13. Added test configuration for a Transcriptome of a Nematode. -14. Now allowed up to 7 days for SYNTENY::DNADIFF based on recent evidence from two ~2.5 GB genomes. - -## Version 0.10.9 (01-Jun-2023) - -1. CRITICAL: Fixed a bug in LAI::EDTA which prevented it from renaming fasta ids in case they were longer than 13 characters. - -## Version 0.10.8 (30-May-2023) - -1. Now NCBI FCS Adaptor and NCBI FCS GX both run in parallel so that both contamination checks are part of the final report even if there is adaptor contamination. - -## Version 0.10.7 (29-May-2023) - -1. CRITICAL: Fixed a bug in LAI::EDTA which prevented it from renaming fasta ids in case they were longer than 13 characters. -2. Now the HiC module does not require the storage_server parameter and the HiC contact map does not disappear when the report is moved across folders. -3. Further developed the tutorials section. -4. Improved presentation of tables for BUSCO and LAI in the report. - -## Version 0.10.6 (25-May-2023) - -1. CRITICAL: Fixed a bug in LAI::EDTA which prevented it from renaming fasta ids in case they were longer than 13 characters. -2. CRITICAL: Fixed a bug in LAI::EDTA which prevented it from accessing the tmp directory. -3. BREAKING: Merged the max_resources config file into the main config file. Slight modifications are required when using the same config file across versions. -4. Now using a central location for assembly_qc singularity containers (/workspace/assembly_qc/singularity) so that individual users don't have to download these containers. -5. Increased resources for the nextflow process so that it can run child processes effectively. -6. Now using nf-core's convention for resource allocation and error strategy. -7. Removed the option to enable hyper-threading. -8. Now only saving the renamed.ids.tsv instead of the whole fasta file from EDTA. -9. Now also saving the EDTA.intact.gff3 file as EDTA sometimes does not store all the annotations in the EDTA.TEanno.gff3 file. - -## Version 0.10.5 (19-May-2023) - -1. CRITICAL: Fixed a bug in RUN_ASSEMBLY_VISUALIZER, HIC_QC introduced by the specification of the temporary directory in version 0.10.4. -2. MATLOCK_BAM2_JUICER now has two hours time limit. -3. Removed dependency on conda. Instead the pipeline now requires vanilla python > 3.7. No specific python packages are required. -4. Started adding detailed tutorials. -5. Now TIDK supports a filter by size parameter to filter out small contigs from it output. By default this filter is turned off. - -## Version 0.10.4 (16-May-2023) - -1. Moved the main workflow into `workflows/assembly_qc.nf` so that it can be imported by other NextFlow pipelines. -2. Fixed a bug in synteny due to which the pipeline did not resume properly sometimes. -3. The included binaries now have unique versions to avoid collision with binaries with same names already present on local PATH. -4. Now using a unique name for the conda environment to have better interoperability across pipelines. -5. Merged configuration files for compiled and max_resources. -6. CRITICAL: Now explicitly setting the temporary directory to avoid "No space left" errors. This problem may have affected container build and NCBI FCS Adaptor/GX modules in the past. -7. Now reporting max_gap and min_bundle size in the report for improved readability. - -## Version 0.10.3 (08-May-2023) - -1. Improved annotation of the config file. -2. Now using natural sort in the synteny color generator so that chr10's color is assigned after chr9's color. -3. Removed global variable definitions in the synteny module in the hope of improving resume-ability. -4. Now all the processes have unique tags. This ensures traceability and resume-ability. -5. CRITICAL: Fixed a bug in the HIC module due to which the pipeline failed to resume properly in some cases. This bug may have also caused mislabelling of the output hic file such that `hap1.hic` may be labelled as `hap2.hic` and vice versa. -6. Added GPLv3 license. -7. Now assembly tags in the dropdown menus of the report are in natural sort order. - -## Version 0.10.2 (04-May-2023) - -1. Allowed 2 hours for DNADIFF and CIRCOS_BUNDLE_LINKS modules. -2. Contigs are now ordered by number on the synteny plot. -3. Added `color_by_contig` option to the synteny module along with a maximum contrast color generator. - -## Version 0.10.1 (28-April-2023) - -1. Fixed a bug in the TIDK module which resulted in genome fasta file emptying in some cases. -2. Added a contributors section to README.md -3. Generalized and simplified configuration parameters and annotations. -4. Fixed a bug in synteny analysis where `between_target_asm` flag had no effect. -5. Updated Juicebox.js to 2.4.3 so that HIC module works behind a VPN. -6. Sorted the list of synteny plots. -7. Removed auto-capitalization of text in the first column of report tables. -8. Fixed a bug in the synteny module which resulted in incorrect inclusion of target sequences in 1-vs-all synteny maps. -9. In the synteny plot, label font size and ticks are now responsive to the number of sequences. -10. Added the `plot_1_vs_all` option in the synteny module. -11. Added `max_gap` and `min_bundle_size` options to the synteny module. - -## Version 0.10 (20-April-2023) - -1. Added Synteny Analysis. -2. Added "-q" and "-qq" option to LAI. "-qq" is the default. -3. Now copying the \*.TElib.fa file from EDTA work dir to the results folder. -4. Fixed the n_limit bug in assemblathon_stats.pl. -5. Now using 4-hour time limit for FASTP and FASTQC. -6. Added references for all the tools in the README. -7. Now the conda environment is saved in the users home directory so that it can be shared across pipeline runs. -8. Updated Juicebox.js to 2.4.1. -9. Allowed 8 hours for BWA MEM. -10. Fixed a bug in LAI where the output was not parsed correctly due to file name mismatch. - -## Version 0.9 (31-Mar-2023) - -1. Added NCBI FCS GX module. -2. Added additional annotation to config file. -3. Removed unnecessary species argument in BUSCO module. -4. Moved NCBI FCS Adaptor/GX scripts to user home directory for sharing across pipeline downloads to different directories. - -## Version 0.8 (29-Mar-2023) - -1. Now using system-wide DBs for BUSCO and KRAKEN2. -2. Added HiC Contact Map module. -3. Further simplified and annotated the config file. - -## Version 0.7.2 (24-Mar-2023) - -1. Fixed a potential bug in ncbi fcs adaptor. -2. Fixed rm -f bug in KRAKEN2. -3. Added additional info for LAI -4. Fixed a few typos in the config file. - -## Version 0.7.1 (23-Mar-2023) - -1. Fixed a bug in the slurm job submission script. -2. Fixed a bug in the ASSEMBLATHON_STATS module. -3. Fixed a bug in SETUP_KRAKEN2_DB module. -4. Now using uniform naming in the TIDK sub-workflow. -5. Max time for LAI now set to 2 hours. - -## Version 0.7 (17-Mar-2023) - -1. Added Kraken2 and NCBI FCS Adaptor tools. -2. Added Assemblathon stats. -3. Added `Genometools gt stat` statistics for gff3 files. -4. Added both a priori and a posteriori sequence search in TIDK. -5. Simplified pipeline flow chart. -6. Simplified conda environment. -7. Fixed css styling browser conflicts -8. TIDK process now uses a container instead of conda. - -## Version 0.6.1 (8-Mar-2023) - -1. Included results_dict and dependencies dict (without html formatting) to json. -2. Removed completed items in readme. -3. Fixed json dump repeating image url. - -## Version 0.6 (17-Feb-2023) - -1. Added LAI. -2. Now sorting sequences by size before feeding to TIDK. -3. Added skip switches for all the tools. -4. Added configuration annotations. -5. Optimised resource allocation. - -## Version 0.5.1 - -1. Changed report parsers to allow alphanumeric ([a-zA-Z0-9_]) characters in the haplotype names. - -## Version 0.5 - -1. Added TIDK - -## Version 0.4 - -1. Added ability run BUSCO for multiple augustus species simultaneously -2. Formatted tabs into a drop down list for ease of navigation -3. Summary page has been added -4. BUSCO plots are now rendered on the summary page -5. Styling has been changed for better user experience - -## Version 0.3 - -1. Added ability to run BUSCO for multiple haplotypes simultaneously -2. Updated README for new functionality -3. Adjusted styling for easier comparisons between reports -4. Incorporated conda instead of python venv - -## Version 0.2 - -1. Added ability to run BUSCO for multiple lineages simultaneously -2. Removed intermediary outputDir -3. Standardised naming conventions across the tool -4. Updated README for new functionality -5. Change report.html layout to tab view +### `Deprecated` diff --git a/CITATION.cff b/CITATION.cff deleted file mode 100644 index efb1b209..00000000 --- a/CITATION.cff +++ /dev/null @@ -1,27 +0,0 @@ -cff-version: 1.2.0 -message: "If you use this pipeline, please cite it as below." -authors: - - family-names: "Rashid" - given-names: "Usman" - orcid: "https://orcid.org/0000-0002-1109-5493" - - family-names: "Wu" - given-names: "Chen" - - family-names: "Shiller" - given-names: "Jason" - - family-names: "Smith" - given-names: "Ken" - - family-names: "Crowhurst" - given-names: "Ross" - - family-names: "Davy" - given-names: "Marcus" - - family-names: "Chen" - given-names: "Ting-Hsuan" - - family-names: "Thomson" - given-names: "Susan" - - family-names: "Deng" - given-names: "Cecilia" -title: "AssemblyQC: A NextFlow pipeline for evaluating assembly quality" -version: 1.4 -date-released: 2024-02-12 -url: "https://github.com/Plant-Food-Research-Open/assembly_qc" -doi: 10.5281/zenodo.10647870 diff --git a/CITATIONS.md b/CITATIONS.md index 19b4531d..2b468811 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -1,106 +1,41 @@ -## Citations +# plant-food-research-open/assemblyqc: Citations -- nf-core/modules, [MIT](https://github.com/nf-core/modules/blob/master/LICENSE) +## [nf-core](https://pubmed.ncbi.nlm.nih.gov/32055031/) - > Ewels PA, Peltzer A, Fillinger S et al. 2020. The nf-core framework for community-curated bioinformatics pipelines. Nat Biotechnol 38, 276–278 (2020). doi: +> Ewels PA, Peltzer A, Fillinger S, Patel H, Alneberg J, Wilm A, Garcia MU, Di Tommaso P, Nahnsen S. The nf-core framework for community-curated bioinformatics pipelines. Nat Biotechnol. 2020 Mar;38(3):276-278. doi: 10.1038/s41587-020-0439-x. PubMed PMID: 32055031. -- py_fasta_validator, [MIT](https://github.com/linsalrob/py_fasta_validator/blob/master/LICENSE) +## [Nextflow](https://pubmed.ncbi.nlm.nih.gov/28398311/) - > Edwards, R.A. 2019. fasta_validate: a fast and efficient fasta validator written in pure C. doi: +> Di Tommaso P, Chatzou M, Floden EW, Barja PP, Palumbo E, Notredame C. Nextflow enables reproducible computational workflows. Nat Biotechnol. 2017 Apr 11;35(4):316-319. doi: 10.1038/nbt.3820. PubMed PMID: 28398311. -- GenomeTools, [ISC](http://genometools.org/license.html) +## Pipeline tools - > Gremme G, Steinbiss S, Kurtz S. 2013. "GenomeTools: A Comprehensive Software Library for Efficient Processing of Structured Genome Annotations," in IEEE/ACM Transactions on Computational Biology and Bioinformatics, vol. 10, no. 3, pp. 645-656, May 2013, doi: +- [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) -- SAMTOOLS, [MIT/Expat](https://github.com/samtools/samtools/blob/develop/LICENSE) + > Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. - > Danecek P, Bonfield JK, Liddle J, Marshall J, Ohan V, Pollard MO, Whitwham A, Keane T, McCarthy SA, Davies RM, Li H. 2021. Twelve years of SAMtools and BCFtools, GigaScience, Volume 10, Issue 2, February 2021, giab008, +- [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) -- NCBI/FCS, [License](https://github.com/ncbi/fcs/blob/main/LICENSE.txt) + > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. - > Astashyn A, Tvedte ES, Sweeney D, Sapojnikov V, Bouk N, Joukov V, Mozes E, Strope PK, Sylla PM, Wagner L, Bidwell SL, Clark K, Davis EW, Smith-White B, Hlavina W, Pruitt KD, Schneider VA, Murphy TD. 2023. Rapid and sensitive detection of genome contamination at scale with FCS-GX. bioRxiv 2023.06.02.543519; doi: +## Software packaging/containerisation tools -- KRONA, [License](https://github.com/marbl/Krona/blob/master/KronaTools/LICENSE.txt) +- [Anaconda](https://anaconda.com) - > Ondov BD, Bergman NH, Phillippy AM. 2011. Interactive metagenomic visualization in a Web browser. BMC Bioinformatics. 2011 Sep 30;12:385. doi: + > Anaconda Software Distribution. Computer software. Vers. 2-2.4.0. Anaconda, Nov. 2016. Web. -- assemblathon_stats, [CC BY-NC-SA 3.0](https://creativecommons.org/licenses/by-nc-sa/3.0/) +- [Bioconda](https://pubmed.ncbi.nlm.nih.gov/29967506/) - > [github/PlantandFoodResearch/assemblathon2-analysis/a93cba2](https://github.com/PlantandFoodResearch/assemblathon2-analysis/blob/a93cba25d847434f7eadc04e63b58c567c46a56d/assemblathon_stats.pl) - > - > Forked from: + > Grüning B, Dale R, Sjödin A, Chapman BA, Rowe J, Tomkins-Tinch CH, Valieris R, Köster J; Bioconda Team. Bioconda: sustainable and comprehensive software distribution for the life sciences. Nat Methods. 2018 Jul;15(7):475-476. doi: 10.1038/s41592-018-0046-7. PubMed PMID: 29967506. -- BUSCO, [MIT](https://gitlab.com/ezlab/busco/-/blob/master/LICENSE) +- [BioContainers](https://pubmed.ncbi.nlm.nih.gov/28379341/) - > Manni M, Berkeley MR, Seppey M, Simão FA, Zdobnov EM. 2021. BUSCO Update: Novel and Streamlined Workflows along with Broader and Deeper Phylogenetic Coverage for Scoring of Eukaryotic, Prokaryotic, and Viral Genomes, Molecular Biology and Evolution, Volume 38, Issue 10, October 2021, Pages 4647–4654, + > da Veiga Leprevost F, Grüning B, Aflitos SA, Röst HL, Uszkoreit J, Barsnes H, Vaudel M, Moreno P, Gatto L, Weber J, Bai M, Jimenez RC, Sachsenberg T, Pfeuffer J, Alvarez RV, Griss J, Nesvizhskii AI, Perez-Riverol Y. BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics. 2017 Aug 15;33(16):2580-2582. doi: 10.1093/bioinformatics/btx192. PubMed PMID: 28379341; PubMed Central PMCID: PMC5870671. -- TIDK, [MIT](https://github.com/tolkit/telomeric-identifier/blob/main/LICENSE) +- [Docker](https://dl.acm.org/doi/10.5555/2600239.2600241) - > + > Merkel, D. (2014). Docker: lightweight linux containers for consistent development and deployment. Linux Journal, 2014(239), 2. doi: 10.5555/2600239.2600241. -- SEQKIT, [MIT](https://github.com/shenwei356/seqkit/blob/master/LICENSE) +- [Singularity](https://pubmed.ncbi.nlm.nih.gov/28494014/) - > Shen W, Le S, Li Y, Hu F. 2016. SeqKit: A Cross-Platform and Ultrafast Toolkit for FASTA/Q File Manipulation. PLoS ONE 11(10): e0163962. - -- LAI, [GPL v3](https://github.com/oushujun/LTR_retriever/blob/master/LICENSE) - - > Ou S, Chen J, Jiang N. 2018. Assessing genome assembly quality using the LTR Assembly Index (LAI), Nucleic Acids Research, Volume 46, Issue 21, 30 November 2018, Page e126, - -- LTR_FINDER_parallel, [MIT](https://github.com/oushujun/LTR_FINDER_parallel/blob/master/LICENSE) - - > Ou S, Jiang N 2019. LTR_FINDER_parallel: parallelization of LTR_FINDER enabling rapid identification of long terminal repeat retrotransposons. Mobile DNA 10, 48 (2019). - -- LTRharvest, [ISC](http://genometools.org/license.html) - - > Ellinghaus, D, Kurtz, S & Willhoeft, U 2008. LTRharvest, an efficient and flexible software for de novo detection of LTR retrotransposons. BMC Bioinformatics 9, 18 (2008). - -- LTR_retriever, [GPL v3](https://github.com/oushujun/LTR_retriever/blob/master/LICENSE) - - > Shujun O, Ning J 2018. LTR_retriever: A Highly Accurate and Sensitive Program for Identification of Long Terminal Repeat Retrotransposons, Plant Physiology, 176, 2 (2018). - -- KRAKEN2, [MIT](https://github.com/DerrickWood/kraken2/blob/master/LICENSE) - - > Wood DE, Salzberg SL, Wood DE, Lu J, Langmead B. 2019. Improved metagenomic analysis with Kraken 2. Genome Biol 20, 257 (2019). - -- JUICEBOX.JS, [MIT](https://github.com/igvteam/juicebox.js/blob/master/LICENSE) - - > Robinson JT, Turner D, Durand NC, Thorvaldsdóttir H, Mesirov JP, Aiden EL. 2018. Juicebox.js Provides a Cloud-Based Visualization System for Hi-C Data. Cell Syst. 2018 Feb 28;6(2):256-258.e1. doi: . Epub 2018 Feb 7. PMID: 29428417; PMCID: PMC6047755. - -- FASTP, [MIT](https://github.com/OpenGene/fastp/blob/master/LICENSE) - - > Chen S, Zhou Y, Chen Y, Gu J. 2018. fastp: an ultra-fast all-in-one FASTQ preprocessor, Bioinformatics, Volume 34, Issue 17, 01 September 2018, Pages i884–i890, - -- FASTQC, [GPL v3](https://github.com/s-andrews/FastQC/blob/master/LICENSE.txt) - - > - -- run-assembly-visualizer.sh, [MIT](https://github.com/aidenlab/3d-dna/blob/master/LICENSE) - - > Dudchenko O, Batra SS, Omer AD, Nyquist SK, Hoeger M, Durand NC, Shamim MS, Machol I, Lander, Aiden AP, Aiden EL 2017. De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds.Science356, 92-95(2017). doi: . Available at: - -- HIC_QC, [AGPL v3](https://github.com/phasegenomics/hic_qc/blob/master/LICENSE) - - > - -- JUICEBOX_SCRIPTS, [AGPL v3](https://github.com/phasegenomics/juicebox_scripts/blob/master/LICENSE) - - > - -- BWA, [GPL v3](https://github.com/lh3/bwa/blob/master/COPYING) - - > Li H. 2013. Aligning sequence reads, clone sequences and assembly contigs with BWA-MEM. - -- MATLOCK, [AGPL v3](https://github.com/phasegenomics/matlock/blob/master/LICENSE) - - > ; - -- SAMBLASTER, [MIT](https://github.com/GregoryFaust/samblaster/blob/master/LICENSE.txt) - - > Faust GG, Hall IM. 2014. SAMBLASTER: fast duplicate marking and structural variant read extraction, Bioinformatics, Volume 30, Issue 17, September 2014, Pages 2503–2505, - -- CIRCOS, [GPL v3](https://www.gnu.org/licenses/gpl-3.0.txt) - - > Krzywinski M, Schein J, Birol I, Connors J, Gascoyne R. Horsman D, ... Marra MA. 2009. Circos: an information aesthetic for comparative genomics. Genome research, 19(9), 1639-1645. - -- MUMMER, [Artistic 2.0](https://github.com/mummer4/mummer/blob/master/LICENSE.md) - > Marçais G, Delcher AL, Phillippy AM, Coston R, Salzberg SL, Zimin A. 2018. MUMmer4: A fast and versatile genome alignment system. PLoS Comput Biol. 2018 Jan 26;14(1):e1005944. doi: . PMID: 29373581; PMCID: PMC5802927. + > Kurtzer GM, Sochat V, Bauer MW. Singularity: Scientific containers for mobility of compute. PLoS One. 2017 May 11;12(5):e0177459. doi: 10.1371/journal.pone.0177459. eCollection 2017. PubMed PMID: 28494014; PubMed Central PMCID: PMC5426675. diff --git a/LEGAL.txt b/LEGAL.txt deleted file mode 100644 index 9e813c16..00000000 --- a/LEGAL.txt +++ /dev/null @@ -1,6083 +0,0 @@ -fasta_validator - -MIT License - -Copyright (c) 2019 Rob Edwards - -Permission is hereby granted, free of charge, to any person obtaining a copy -of this software and associated documentation files (the "Software"), to deal -in the Software without restriction, including without limitation the rights -to use, copy, modify, merge, publish, distribute, sublicense, and/or sell -copies of the Software, and to permit persons to whom the Software is -furnished to do so, subject to the following conditions: - -The above copyright notice and this permission notice shall be included in all -copies or substantial portions of the Software. - -THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR -IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, -FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. 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Disclaimer of Warranty. - - THERE IS NO WARRANTY FOR THE PROGRAM, TO THE EXTENT PERMITTED BY -APPLICABLE LAW. EXCEPT WHEN OTHERWISE STATED IN WRITING THE COPYRIGHT -HOLDERS AND/OR OTHER PARTIES PROVIDE THE PROGRAM "AS IS" WITHOUT WARRANTY -OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING, BUT NOT LIMITED TO, -THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR -PURPOSE. THE ENTIRE RISK AS TO THE QUALITY AND PERFORMANCE OF THE PROGRAM -IS WITH YOU. SHOULD THE PROGRAM PROVE DEFECTIVE, YOU ASSUME THE COST OF -ALL NECESSARY SERVICING, REPAIR OR CORRECTION. - - 16. 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Of course, your program's commands -might be different; for a GUI interface, you would use an "about box". - - You should also get your employer (if you work as a programmer) or school, -if any, to sign a "copyright disclaimer" for the program, if necessary. -For more information on this, and how to apply and follow the GNU GPL, see -. - - The GNU General Public License does not permit incorporating your program -into proprietary programs. If your program is a subroutine library, you -may consider it more useful to permit linking proprietary applications with -the library. If this is what you want to do, use the GNU Lesser General -Public License instead of this License. But first, please read -. - -=============================================================================== - -EDTA - - GNU GENERAL PUBLIC LICENSE - Version 3, 29 June 2007 - - Copyright (C) 2007 Free Software Foundation, Inc. - Everyone is permitted to copy and distribute verbatim copies - of this license document, but changing it is not allowed. - - Preamble - - The GNU General Public License is a free, copyleft license for -software and other kinds of works. - - The licenses for most software and other practical works are designed -to take away your freedom to share and change the works. By contrast, -the GNU General Public License is intended to guarantee your freedom to -share and change all versions of a program--to make sure it remains free -software for all its users. We, the Free Software Foundation, use the -GNU General Public License for most of our software; it applies also to -any other work released this way by its authors. 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But first, please read -. - -=============================================================================== - -KRAKEN2 - -The MIT License (MIT) - -Copyright (c) 2017-2023 Derrick Wood - -Permission is hereby granted, free of charge, to any person obtaining a copy -of this software and associated documentation files (the "Software"), to deal -in the Software without restriction, including without limitation the rights -to use, copy, modify, merge, publish, distribute, sublicense, and/or sell -copies of the Software, and to permit persons to whom the Software is -furnished to do so, subject to the following conditions: - -The above copyright notice and this permission notice shall be included in all -copies or substantial portions of the Software. - -THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR -IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, -FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. 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If the Program does not specify a version number of the -GNU Affero General Public License, you may choose any version ever published -by the Free Software Foundation. - - If the Program specifies that a proxy can decide which future -versions of the GNU Affero General Public License can be used, that proxy's -public statement of acceptance of a version permanently authorizes you -to choose that version for the Program. - - Later license versions may give you additional or different -permissions. However, no additional obligations are imposed on any -author or copyright holder as a result of your choosing to follow a -later version. - - 15. Disclaimer of Warranty. - - THERE IS NO WARRANTY FOR THE PROGRAM, TO THE EXTENT PERMITTED BY -APPLICABLE LAW. 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It is safest -to attach them to the start of each source file to most effectively -state the exclusion of warranty; and each file should have at least -the "copyright" line and a pointer to where the full notice is found. - - - Copyright (C) - - This program is free software: you can redistribute it and/or modify - it under the terms of the GNU General Public License as published by - the Free Software Foundation, either version 3 of the License, or - (at your option) any later version. - - This program is distributed in the hope that it will be useful, - but WITHOUT ANY WARRANTY; without even the implied warranty of - MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the - GNU General Public License for more details. - - You should have received a copy of the GNU General Public License - along with this program. If not, see . - -Also add information on how to contact you by electronic and paper mail. - - If the program does terminal interaction, make it output a short -notice like this when it starts in an interactive mode: - - Copyright (C) - This program comes with ABSOLUTELY NO WARRANTY; for details type `show w'. - This is free software, and you are welcome to redistribute it - under certain conditions; type `show c' for details. - -The hypothetical commands `show w' and `show c' should show the appropriate -parts of the General Public License. Of course, your program's commands -might be different; for a GUI interface, you would use an "about box". - - You should also get your employer (if you work as a programmer) or school, -if any, to sign a "copyright disclaimer" for the program, if necessary. -For more information on this, and how to apply and follow the GNU GPL, see -. - - The GNU General Public License does not permit incorporating your program -into proprietary programs. If your program is a subroutine library, you -may consider it more useful to permit linking proprietary applications with -the library. If this is what you want to do, use the GNU Lesser General -Public License instead of this License. But first, please read -. +MIT License + +Copyright (c) Usman Rashid + +Permission is hereby granted, free of charge, to any person obtaining a copy +of this software and associated documentation files (the "Software"), to deal +in the Software without restriction, including without limitation the rights +to use, copy, modify, merge, publish, distribute, sublicense, and/or sell +copies of the Software, and to permit persons to whom the Software is +furnished to do so, subject to the following conditions: + +The above copyright notice and this permission notice shall be included in all +copies or substantial portions of the Software. + +THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR +IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, +FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE +AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER +LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, +OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE +SOFTWARE. diff --git a/MANIFEST.json b/MANIFEST.json deleted file mode 100644 index 7a525a2c..00000000 --- a/MANIFEST.json +++ /dev/null @@ -1,5 +0,0 @@ -{ - "mainWorkflowURL": "main.nf", - "inputFileURLs": ["./docs/test_params/test_agc.json"], - "engineOptions": "-resume" -} diff --git a/README.md b/README.md index 8564d306..92b64574 100644 --- a/README.md +++ b/README.md @@ -1,131 +1,90 @@ -# AssemblyQC - -[![DOI](https://zenodo.org/badge/DOI/10.5281/zenodo.10647870.svg)](https://doi.org/10.5281/zenodo.10647870) - -- [AssemblyQC](#assemblyqc) - - [Introduction](#introduction) - - [Pipeline Flowchart](#pipeline-flowchart) - - [Running the Pipeline](#running-the-pipeline) - - [Minimal Test Run](#minimal-test-run) - - [Quick Start for Plant \& Food Research Users](#quick-start-for-plant--food-research-users) - - [Post-run clean-up](#post-run-clean-up) - - [AssemblyQC Report](#assemblyqc-report) - - [Known Issues](#known-issues) - - [Contributors](#contributors) - - [Citations](#citations) +[![GitHub Actions CI Status](https://github.com/plant-food-research-open/assemblyqc/workflows/nf-core%20CI/badge.svg)](https://github.com/plant-food-research-open/assemblyqc/actions?query=workflow%3A%22nf-core+CI%22) +[![GitHub Actions Linting Status](https://github.com/plant-food-research-open/assemblyqc/workflows/nf-core%20linting/badge.svg)](https://github.com/plant-food-research-open/assemblyqc/actions?query=workflow%3A%22nf-core+linting%22)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) -## Introduction - -AssemblyQC is a [NextFlow](https://www.nextflow.io/docs/latest/index.html) pipeline which evaluates assembly quality with well-established tools and presents the results in a unified html report. The tools are shown in the [Pipeline Flowchart](#pipeline-flowchart) and their version are listed in [CITATIONS.md](./CITATIONS.md). - -## Pipeline Flowchart +[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) +[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) +[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) +[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) +[![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/plant-food-research-open/assemblyqc) -```mermaid -flowchart LR - forEachTag(For each\nAssembly) --> VALIDATE_FORMAT[VALIDATE FORMAT] - - VALIDATE_FORMAT --> ncbiFCS[NCBI FCS\nADAPTOR] - ncbiFCS --> Check{Check} +## Introduction - VALIDATE_FORMAT --> ncbiGX[NCBI FCS GX] - ncbiGX --> Check - Check --> |Clean|Run(Run) +**plant-food-research-open/assemblyqc** is a bioinformatics pipeline that ... - Check --> |Contamination|Skip(Skip All) - Skip --> REPORT + - VALIDATE_FORMAT --> GFF_STATS[GENOMETOOLS GT STAT] + + - Run --> ASS_STATS[ASSEMBLATHON STATS] - Run --> BUSCO - Run --> TIDK - Run --> LTRRETRIEVER - LTRRETRIEVER --> LAI - Run --> KRAKEN2 - Run --> HIC_CONTACT_MAP[HIC CONTACT MAP] - Run --> SYNTENY +1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) +2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/)) - ASS_STATS --> REPORT - GFF_STATS --> REPORT - BUSCO --> REPORT - TIDK --> REPORT - LAI --> REPORT - KRAKEN2 --> REPORT - HIC_CONTACT_MAP --> REPORT - SYNTENY --> REPORT -``` +## Usage -- [FASTA VALIDATION](https://github.com/GallVp/fasta_validator) -- [GFF3 VALIDATION](https://github.com/genometools/genometools) -- [ASSEMBLATHON STATS](https://github.com/PlantandFoodResearch/assemblathon2-analysis/blob/a93cba25d847434f7eadc04e63b58c567c46a56d/assemblathon_stats.pl): Assembly statistics -- [GENOMETOOLS GT STAT](https://github.com/genometools/genometools): Annotation statistics -- [NCBI FCS ADAPTOR](https://github.com/ncbi/fcs): Adaptor contamination pass/fail -- [NCBI FCS GX](https://github.com/ncbi/fcs): Foreign organism contamination pass/fail -- [BUSCO](https://gitlab.com/ezlab/busco/-/tree/master): Gene-space completeness estimation -- [TIDK](https://github.com/tolkit/telomeric-identifier): Telomere repeat identification -- [LAI](https://github.com/oushujun/LTR_retriever/blob/master/LAI): Continuity of repetitive sequences -- [LAI::LTRRETRIEVER](https://github.com/oushujun/LTR_retriever): Repeat identification -- [KRAKEN2](https://github.com/DerrickWood/kraken2): Taxonomy classification -- [HIC CONTACT MAP](https://github.com/igvteam/juicebox-web): Alignment and visualisation of HiC data -- SYNTENY: Synteny analysis using [MUMMER](https://github.com/mummer4/mummer) and [CIRCOS](http://circos.ca/documentation/) +> [!NOTE] +> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. -## Running the Pipeline + -- target_assemblies -- assembly_gff3 -- assemblathon_stats::n_limit -- ncbi_fcs_adaptor::empire -- ncbi_fcs_gx::tax_id -- busco::lineage_datasets -- busco::mode -- tidk::repeat_seq -- hic::paired_reads -- synteny::assembly_seq_list -- synteny::xref_assemblies +Now, you can run the pipeline using: -Then, the pipeline should be posted to Slurm for execution with the following command: + ```bash -sbatch ./pfr_assemblyqc +nextflow run plant-food-research-open/assemblyqc \ + -profile \ + --input samplesheet.csv \ + --outdir ``` -### Post-run clean-up +> [!WARNING] +> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; +> see [docs](https://nf-co.re/usage/configuration#custom-configuration-files). -The intermediary files produced by the pipeline are stored in the "work" folder. After running the pipeline, if you wish to clean up the logs and "work" folder, run the following command: +## Credits -```bash -./cleanNXF.sh -``` +plant-food-research-open/assemblyqc was originally written by Usman Rashid. -## AssemblyQC Report +We thank the following people for their extensive assistance in the development of this pipeline: -Once the pipeline has finished execution, the results folder specified in the config file should contain a file named 'report.html'. The 'report.html' is a standalone file for all the modules except HiC and Kraken2. Thus, if you move the report to another folder, make sure to also move the 'hic' folder and the 'kraken2' folder with it. + -## Known Issues +## Contributions and Support -- On its first run, the pipeline has to download a lot many software containers. This download may fail. If it happens, resume the pipeline and it should be able to download the required containers. -- The pipeline may fail more frequently at building singularity containers when the temporary directory is not the system "/tmp" directory. +If you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md). -## Contributors +## Citations -Cecilia Deng [@CeciliaDeng](https://github.com/CeciliaDeng), Chen Wu [@christinawu2008](https://github.com/christinawu2008), Jason Shiller [@jasonshiller](https://github.com/jasonshiller), Ken Smith [@hzlnutspread](https://github.com/hzlnutspread), Marcus Davy [@mdavy86](https://github.com/mdavy86), Ross Crowhurst [@rosscrowhurst](https://github.com/rosscrowhurst), Susan Thomson [@cflsjt](https://github.com/cflsjt), Ting-Hsuan Chen [@ting-hsuan-chen](https://github.com/ting-hsuan-chen), Usman Rashid [@GallVp](https://github.com/GallVp) + + -## Citations + + +An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file. -For a comprehensive list of references and versions for the tools, see [CITATIONS.md](./CITATIONS.md). +This pipeline uses code and infrastructure developed and maintained by the [nf-core](https://nf-co.re) community, reused here under the [MIT license](https://github.com/nf-core/tools/blob/master/LICENSE). -> Rashid, U., Wu, C., Shiller, J., Smith, K., Crowhurst, R., Davy, M., Chen, T.-H., Thomson, S., & Deng, C. (2024). AssemblyQC: A NextFlow pipeline for evaluating assembly quality (1.4). Zenodo. https://doi.org/10.5281/zenodo.10647870 +> **The nf-core framework for community-curated bioinformatics pipelines.** +> +> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen. +> +> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x). diff --git a/agc-project.yaml b/agc-project.yaml deleted file mode 100644 index 7d7cc54d..00000000 --- a/agc-project.yaml +++ /dev/null @@ -1,24 +0,0 @@ -name: AssemblyQC -schemaVersion: 1 -workflows: - PFR_ASSEMBLY_QC: - type: - language: nextflow - version: 1.0 - sourceURL: ./ -contexts: - CtxAssemblyQC: - instanceTypes: - - "c5.large" - - "c5.2xlarge" - - "c5.4xlarge" - - "m5.2xlarge" - - "m5.4xlarge" - - "r5.2xlarge" - - "r5.4xlarge" - - "r5.8xlarge" # process_high_memory (200.GB) -> { 32, 256 } - - "r5.24xlarge" # process_very_high_memory (512.GB) -> { 96, 768 } - engines: - - type: nextflow - engine: nextflow - requestSpotInstances: true diff --git a/assets/adaptivecard.json b/assets/adaptivecard.json new file mode 100644 index 00000000..6477ced0 --- /dev/null +++ b/assets/adaptivecard.json @@ -0,0 +1,67 @@ +{ + "type": "message", + "attachments": [ + { + "contentType": "application/vnd.microsoft.card.adaptive", + "contentUrl": null, + "content": { + "\$schema": "http://adaptivecards.io/schemas/adaptive-card.json", + "msteams": { + "width": "Full" + }, + "type": "AdaptiveCard", + "version": "1.2", + "body": [ + { + "type": "TextBlock", + "size": "Large", + "weight": "Bolder", + "color": "<% if (success) { %>Good<% } else { %>Attention<%} %>", + "text": "plant-food-research-open/assemblyqc v${version} - ${runName}", + "wrap": true + }, + { + "type": "TextBlock", + "spacing": "None", + "text": "Completed at ${dateComplete} (duration: ${duration})", + "isSubtle": true, + "wrap": true + }, + { + "type": "TextBlock", + "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors. The full error message was: ${errorReport}.<% } %>", + "wrap": true + }, + { + "type": "TextBlock", + "text": "The command used to launch the workflow was as follows:", + "wrap": true + }, + { + "type": "TextBlock", + "text": "${commandLine}", + "isSubtle": true, + "wrap": true + } + ], + "actions": [ + { + "type": "Action.ShowCard", + "title": "Pipeline Configuration", + "card": { + "type": "AdaptiveCard", + "\$schema": "http://adaptivecards.io/schemas/adaptive-card.json", + "body": [ + { + "type": "FactSet", + "facts": [<% out << summary.collect{ k,v -> "{\"title\": \"$k\", \"value\" : \"$v\"}"}.join(",\n") %> + ] + } + ] + } + } + ] + } + } + ] +} diff --git a/assets/email_template.html b/assets/email_template.html new file mode 100644 index 00000000..fa1b2da2 --- /dev/null +++ b/assets/email_template.html @@ -0,0 +1,53 @@ + + + + + + + + plant-food-research-open/assemblyqc Pipeline Report + + +
+ + + +

plant-food-research-open/assemblyqc ${version}

+

Run Name: $runName

+ +<% if (!success){ + out << """ +
+

plant-food-research-open/assemblyqc execution completed unsuccessfully!

+

The exit status of the task that caused the workflow execution to fail was: $exitStatus.

+

The full error message was:

+ 
${errorReport}
+
+ """ +} else { + out << """ +
+ plant-food-research-open/assemblyqc execution completed successfully! +
+ """ +} +%> + +

The workflow was completed at $dateComplete (duration: $duration)

+

The command used to launch the workflow was as follows:

+
$commandLine
+ +

Pipeline Configuration:

+
Process Name \\", + " \\ Software Version
CUSTOM_DUMPSOFTWAREVERSIONSpython3.11.7
yaml5.4.1
TOOL1tool10.11.9
TOOL2tool21.9
WorkflowNextflow
+ + <% out << summary.collect{ k,v -> "" }.join("\n") %> + +
$k
$v
+ +

plant-food-research-open/assemblyqc

+

https://github.com/plant-food-research-open/assemblyqc

+ + + + + diff --git a/assets/email_template.txt b/assets/email_template.txt new file mode 100644 index 00000000..720fd719 --- /dev/null +++ b/assets/email_template.txt @@ -0,0 +1,31 @@ +Run Name: $runName + +<% if (success){ + out << "## plant-food-research-open/assemblyqc execution completed successfully! ##" +} else { + out << """#################################################### +## plant-food-research-open/assemblyqc execution completed unsuccessfully! ## +#################################################### +The exit status of the task that caused the workflow execution to fail was: $exitStatus. +The full error message was: + +${errorReport} +""" +} %> + + +The workflow was completed at $dateComplete (duration: $duration) + +The command used to launch the workflow was as follows: + + $commandLine + + + +Pipeline Configuration: +----------------------- +<% out << summary.collect{ k,v -> " - $k: $v" }.join("\n") %> + +-- +plant-food-research-open/assemblyqc +https://github.com/plant-food-research-open/assemblyqc diff --git a/assets/methods_description_template.yml b/assets/methods_description_template.yml new file mode 100644 index 00000000..e2a5645c --- /dev/null +++ b/assets/methods_description_template.yml @@ -0,0 +1,29 @@ +id: "plant-food-research-open-assemblyqc-methods-description" +description: "Suggested text and references to use when describing pipeline usage within the methods section of a publication." +section_name: "plant-food-research-open/assemblyqc Methods Description" +section_href: "https://github.com/plant-food-research-open/assemblyqc" +plot_type: "html" +## TODO nf-core: Update the HTML below to your preferred methods description, e.g. add publication citation for this pipeline +## You inject any metadata in the Nextflow '${workflow}' object +data: | +

Methods

+

Data was processed using plant-food-research-open/assemblyqc v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

+

The pipeline was executed with Nextflow v${workflow.nextflow.version} (Di Tommaso et al., 2017) with the following command:

+ 
${workflow.commandLine}
+

${tool_citations}

+

References

+ +
+
Notes:
+ +
diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml new file mode 100644 index 00000000..6d8df9b7 --- /dev/null +++ b/assets/multiqc_config.yml @@ -0,0 +1,12 @@ +report_comment: > + This report has been generated by the plant-food-research-open/assemblyqc + analysis pipeline. +report_section_order: + "plant-food-research-open-assemblyqc-methods-description": + order: -1000 + software_versions: + order: -1001 + "plant-food-research-open-assemblyqc-summary": + order: -1002 + +export_plots: true diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv new file mode 100644 index 00000000..5f653ab7 --- /dev/null +++ b/assets/samplesheet.csv @@ -0,0 +1,3 @@ +sample,fastq_1,fastq_2 +SAMPLE_PAIRED_END,/path/to/fastq/files/AEG588A1_S1_L002_R1_001.fastq.gz,/path/to/fastq/files/AEG588A1_S1_L002_R2_001.fastq.gz +SAMPLE_SINGLE_END,/path/to/fastq/files/AEG588A4_S4_L003_R1_001.fastq.gz, diff --git a/assets/schema_input.json b/assets/schema_input.json new file mode 100644 index 00000000..cff17406 --- /dev/null +++ b/assets/schema_input.json @@ -0,0 +1,36 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/master/assets/schema_input.json", + "title": "plant-food-research-open/assemblyqc pipeline - params.input schema", + "description": "Schema for the file provided with params.input", + "type": "array", + "items": { + "type": "object", + "properties": { + "sample": { + "type": "string", + "pattern": "^\\S+$", + "errorMessage": "Sample name must be provided and cannot contain spaces" + }, + "fastq_1": { + "type": "string", + "pattern": "^\\S+\\.f(ast)?q\\.gz$", + "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" + }, + "fastq_2": { + "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'", + "anyOf": [ + { + "type": "string", + "pattern": "^\\S+\\.f(ast)?q\\.gz$" + }, + { + "type": "string", + "maxLength": 0 + } + ] + } + }, + "required": ["sample", "fastq_1"] + } +} diff --git a/assets/sendmail_template.txt b/assets/sendmail_template.txt new file mode 100644 index 00000000..0ba5f393 --- /dev/null +++ b/assets/sendmail_template.txt @@ -0,0 +1,53 @@ +To: $email +Subject: $subject +Mime-Version: 1.0 +Content-Type: multipart/related;boundary="nfcoremimeboundary" + +--nfcoremimeboundary +Content-Type: text/html; charset=utf-8 + +$email_html + +--nfcoremimeboundary +Content-Type: image/png;name="plant-food-research-open-assemblyqc_logo.png" +Content-Transfer-Encoding: base64 +Content-ID: +Content-Disposition: inline; filename="plant-food-research-open-assemblyqc_logo_light.png" + +<% out << new File("$projectDir/assets/plant-food-research-open-assemblyqc_logo_light.png"). + bytes. + encodeBase64(). + toString(). + tokenize( '\n' )*. + toList()*. + collate( 76 )*. + collect { it.join() }. + flatten(). + join( '\n' ) %> + +<% +if (mqcFile){ +def mqcFileObj = new File("$mqcFile") +if (mqcFileObj.length() < mqcMaxSize){ +out << """ +--nfcoremimeboundary +Content-Type: text/html; name=\"multiqc_report\" +Content-Transfer-Encoding: base64 +Content-ID: +Content-Disposition: attachment; filename=\"${mqcFileObj.getName()}\" + +${mqcFileObj. + bytes. + encodeBase64(). + toString(). + tokenize( '\n' )*. + toList()*. + collate( 76 )*. + collect { it.join() }. + flatten(). + join( '\n' )} +""" +}} +%> + +--nfcoremimeboundary-- diff --git a/assets/slackreport.json b/assets/slackreport.json new file mode 100644 index 00000000..2786203f --- /dev/null +++ b/assets/slackreport.json @@ -0,0 +1,34 @@ +{ + "attachments": [ + { + "fallback": "Plain-text summary of the attachment.", + "color": "<% if (success) { %>good<% } else { %>danger<%} %>", + "author_name": "plant-food-research-open/assemblyqc ${version} - ${runName}", + "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico", + "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>", + "fields": [ + { + "title": "Command used to launch the workflow", + "value": "```${commandLine}```", + "short": false + } + <% + if (!success) { %> + , + { + "title": "Full error message", + "value": "```${errorReport}```", + "short": false + }, + { + "title": "Pipeline configuration", + "value": "<% out << summary.collect{ k,v -> k == "hook_url" ? "_${k}_: (_hidden_)" : ( ( v.class.toString().contains('Path') || ( v.class.toString().contains('String') && v.contains('/') ) ) ? "_${k}_: `${v}`" : (v.class.toString().contains('DateTime') ? ("_${k}_: " + v.format(java.time.format.DateTimeFormatter.ofLocalizedDateTime(java.time.format.FormatStyle.MEDIUM))) : "_${k}_: ${v}") ) }.join(",\n") %>", + "short": false + } + <% } + %> + ], + "footer": "Completed at <% out << dateComplete.format(java.time.format.DateTimeFormatter.ofLocalizedDateTime(java.time.format.FormatStyle.MEDIUM)) %> (duration: ${duration})" + } + ] +} diff --git a/bin/FAlite_943e0fb.pm b/bin/FAlite_943e0fb.pm deleted file mode 100644 index 262728b4..00000000 --- a/bin/FAlite_943e0fb.pm +++ /dev/null @@ -1,128 +0,0 @@ -package FAlite_943e0fb; -use strict; -sub new { - my ($class, $fh) = @_; - if (ref $fh !~ /GLOB/) - {die ref $fh, "\n", "FAlite_943e0fb ERROR: expect a GLOB reference\n"} - my $this = bless {}; - $this->{FH} = $fh; - while(<$fh>) {last if $_ =~ /\S/} # not supposed to have blanks, but... - my $firstline = $_; - if (not defined $firstline) {warn "FAlite_943e0fb: Empty\n"; return $this} - if ($firstline !~ /^>/) {warn "FAlite_943e0fb: Not FASTA formatted\n"; return $this} - $this->{LASTLINE} = $firstline; - chomp $this->{LASTLINE}; - return $this; -} -sub nextEntry { - my ($this) = @_; - return 0 if not defined $this->{LASTLINE}; - my $fh = $this->{FH}; - my $def = $this->{LASTLINE}; - my @seq; - my $lines_read = 0; - while(<$fh>) { - $lines_read++; - if ($_ =~ /^>/) { - $this->{LASTLINE} = $_; - chomp $this->{LASTLINE}; - last; - } - push @seq, $_; - } - return 0 if $lines_read == 0; - chomp @seq; - my $entry = FAlite_943e0fb::Entry::new($def, \@seq); - return $entry; -} - -package FAlite_943e0fb::Entry; -use overload '""' => 'all'; -sub new { - my ($def, $seqarry) = @_; - my $this = bless {}; - $this->{DEF} = $def; - $this->{SEQ} = join("", @$seqarry); - $this->{SEQ} =~ s/\s//g; # just in case more spaces - return $this; -} -sub def {shift->{DEF}} -sub seq {shift->{SEQ}} -sub all {my $e = shift; return $e->{DEF}."\n".$e->{SEQ}."\n"} - -1; - -__END__ - -=head1 NAME - -FAlite_943e0fb; - -=head1 SYNOPSIS - - use FAlite_943e0fb; - my $fasta = new FAlite_943e0fb(\*STDIN); - while(my $entry = $fasta->nextEntry) { - $entry->def; - $entry->seq; - } - -=head1 DESCRIPTION - -FAlite_943e0fb is a package for parsing FASTA files and databases. The FASTA format is -widely used in bioinformatics. It consists of a definition line followed by -sequence with an arbitrary number of lines and line lengths. - -A FASTA file looks like this: - - >identifier descriptive text - GAATTC - -A FASTA database looks like this: - - >identifier1 some text describing this entry - GAATTC - ACTAGT - >identifier2 some text describing this entry - AAACCT - GCTAAT - -=head2 Object - -FAlite_943e0fb has two kinds of objects, the file and the entry. - - my $fasta_file = new FAlite_943e0fb(\*STDIN); # or any other filehandle - $entry = $fasta_file->nextEntry; # single fasta fle - while(my $entry = $fasta_file->nextEntry) { - # canonical form of use for fasta database - } - -The entry has two attributes (def and seq). - - $entry->def; # access the def line - $entry->seq; # access the sequence - "$entry"; # overload to fasta file ($entry->def . "\n" . $entry->seq) - -=head1 AUTHOR - -Ian Korf (ikorf@sapiens.wustl.edu, http://sapiens.wustl.edu/~ikorf) - -=head1 ACKNOWLEDGEMENTS - -This software was developed at the Genome Sequencing Center at Washington -Univeristy, St. Louis, MO. - -=head1 COPYRIGHT - -Copyright (C) 1999 Ian Korf. All Rights Reserved. - -=head1 DISCLAIMER - -This software is provided "as is" without warranty of any kind. - -=cut - - - - - diff --git a/bin/add_color_2_circos_bundle_file_943e0fb.pl b/bin/add_color_2_circos_bundle_file_943e0fb.pl deleted file mode 100755 index 8050e3c0..00000000 --- a/bin/add_color_2_circos_bundle_file_943e0fb.pl +++ /dev/null @@ -1,181 +0,0 @@ -#!/usr/bin/perl -use strict; -use warnings; - -=head1 DESCRIPTION - -Adds colours to a CIRCOS bundle file. - -=head1 AUTHOR - -Original: Ross Crowhurst L -Modified: Usman Rashid L - -=cut - -my $low = 0; - -my %bundleColorsRGB = ( - 3000 => "128,0,0,0.5", - 2000 => "229,0,10,0.5", - 1500 => "229,19,9,0.5", - 1000 => "216,38,8,0.5", - 500 => "210,57,7,0.5", - 250 => "204,76,6,0.5", - 100 => "198,95,5,0.5", - 50 => "192,114,4,0.5", - 25 => "186,113,3,0.5", - 10 => "180,152,2,0.5", - 5 => "174,171,1,0.5", - 0 => "168,191,0,0.5" -); - -my %bundleColorsRGBLow = ( - 55 => "128,0,0,0.5", - 50 => "229,0,10,0.5", - 45 => "229,19,9,0.5", - 40 => "216,38,8,0.5", - 35 => "210,57,7,0.5", - 30 => "204,76,6,0.5", - 25 => "198,95,5,0.5", - 20 => "192,114,4,0.5", - 15 => "186,113,3,0.5", - 10 => "180,152,2,0.5", - 5 => "174,171,1,0.5", - 0 => "168,191,0,0.5" -); - -sub usage { - print "USAGE: $0 -i=bundle_file_in -o=colored_bundle_file_out [-low]\n"; - print "To get colors:\n\n"; - print " $0 -colorsRGB [or -colorsRGBAsHTMLTable] [-low]\n"; - print "or\n"; - print " $0 -colorsHex [-low]\n"; - print "or\n"; - print " $0 -colorsHexAsHTMLKeyTable [-low]\n"; - exit(0); -} - -sub exportRGB { - if ($low) - { - foreach my $threshold (sort {$a <=> $b} keys %bundleColorsRGBLow) - { - print "$threshold\t$bundleColorsRGBLow{$threshold}\n"; - } - } - else - { - foreach my $threshold (sort {$a <=> $b} keys %bundleColorsRGB) - { - print "$threshold\t$bundleColorsRGB{$threshold}\n"; - } - } - exit(0); -} - -sub exportRGBHTMLTable { - print "\n"; - print "\n"; - if ($low) - { - foreach my $threshold (sort {$a <=> $b} keys %bundleColorsRGBLow) - { - my $cellBgColor = rgbToHex($bundleColorsRGBLow{$threshold}); - print qq{\n}; - } - } - else - { - foreach my $threshold (sort {$a <=> $b} keys %bundleColorsRGB) - { - my $cellBgColor = rgbToHex($bundleColorsRGB{$threshold}); - print qq{\n}; - } - } - print "
Bundled LinksRGB
$threshold$bundleColorsRGBLow{$threshold}
$threshold$bundleColorsRGB{$threshold}
\n"; - exit(0); -} - -sub exportAsHTMLKeyTable { - print "\n"; - print "\n"; - if ($low) - { - foreach my $threshold (sort {$a <=> $b} keys %bundleColorsRGBLow) - { - my $cellBgColor = rgbToHex($bundleColorsRGBLow{$threshold}); - print qq{\n}; - } - } - else - { - foreach my $threshold (sort {$a <=> $b} keys %bundleColorsRGB) - { - my $cellBgColor = rgbToHex($bundleColorsRGB{$threshold}); - print qq{\n}; - } - } - print "
Bundled Links
 $threshold
 $threshold
\n"; - exit(0); -} -sub rgbToHex { - my ($r, $g, $b) = split/,/, $_[0]; - return sprintf ("#%2.2X%2.2X%2.2X", $r, $g, $b); -} - -my $bundleFileIn = ""; -my $bundleFileOut = ""; - -(@ARGV) or usage(); -foreach my $arg (@ARGV) -{ - ($arg =~ m/^-(h|help)$/) and usage(); - ($arg =~ m/^-low$/) and $low = 1; - ($arg =~ m/^-colorsRGB$/) and exportRGB(); - ($arg =~ m/^-colorsRGBAsHTMLTable$/) and exportRGBHTMLTable(); - ($arg =~ m/^-colorsHexAsHTMLKeyTable$/) and exportAsHTMLKeyTable(); - ($arg =~ m/^-i=(.+)$/) and $bundleFileIn = $1; - ($arg =~ m/^-o=(.+)$/) and $bundleFileOut = $1; -} - -open(OUT, ">$bundleFileOut") or die "ERROR: can not open bundle out file $bundleFileOut $!\n"; -open(IN, "<$bundleFileIn") or die "ERROR: can not open bundle in file $bundleFileIn $!\n"; -while (my $line = ) -{ -#ASB_LG19 13470754 14218750 Ss262 2177839 2976275 nlinks=672,bsize1=150447,bsize2=150419,bidentity1=0.201133,bidentity2=0.188392,depth1=0,depth2=0, -#ASB_LG19 14250080 15061508 Ss262 1303606 2191377 nlinks=1076,bsize1=279892,bsize2=278553,bidentity1=0.344937,bidentity2=0.313766,depth1=0,depth2=0, -#ASB_LG19 14314359 14314420 Ss262 7198136 7198167 nlinks=9,bsize1=62,bsize2=32,bidentity1=1.000000,bidentity2=1.000000,depth1=1,depth2=1, -#ASB_LG19 15064224 15625360 Ss262 672993 1254783 nlinks=881,bsize1=305520,bsize2=304727,bidentity1=0.544466,bidentity2=0.523774,depth1=0,depth2=0, -#ASB_LG19 15650721 16282135 Ss262 8995 672359 nlinks=786,bsize1=199405,bsize2=198505,bidentity1=0.315807,bidentity2=0.299239,depth1=0,depth2=0, -#ASB_LG19 17026943 17042421 Ss262 965 7848 nlinks=35,bsize1=7610,bsize2=4363,bidentity1=0.491634,bidentity2=0.633788,depth1=0,depth2=0, - chomp $line; - my @data = split/\s+/, $line; - my @bundleFields = split/,/, $data[6]; - my ($label, $count) = split/=/, $bundleFields[0]; - my $colorText = "color=(168,191,0)"; - if ($low) - { - foreach my $threshold (sort {$a <=> $b} keys %bundleColorsRGBLow) - { - if ($count > $threshold) - { - $colorText = "color=($bundleColorsRGBLow{$threshold})"; - } - } - } - else - { - foreach my $threshold (sort {$a <=> $b} keys %bundleColorsRGB) - { - if ($count > $threshold) - { - $colorText = "color=($bundleColorsRGB{$threshold})"; - } - } - } - my $newline = join(" ", $data[0], $data[1], $data[2], $data[3], $data[4], $data[5], $colorText, $data[6]); - select OUT; print OUT "$newline\n"; -} -close(OUT); -exit(0); diff --git a/bin/assemblathon_stats_943e0fb.pl b/bin/assemblathon_stats_943e0fb.pl deleted file mode 100755 index 002d121d..00000000 --- a/bin/assemblathon_stats_943e0fb.pl +++ /dev/null @@ -1,528 +0,0 @@ -#!/usr/bin/perl -# -# assemblathon_stats.pl -# -# A script to calculate a basic set of metrics from a genome assembly -# -# Author: Keith Bradnam, Genome Center, UC Davis -# This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. -# This software is provided AS IS, without warranty of any kind. - -use strict; -use warnings; -use FAlite_943e0fb; -use Getopt::Long; -use List::Util qw(sum max min); - -############################################### -# -# C o m m a n d l i n e o p t i o n s -# -############################################### - -my $limit; # limit processing of data to first $limit sequences (for quick testing) -my $graph; # produce some output ready for Excel or R -my $csv; # produce CSV output file of results -my $n_limit; # how many N characters should be used to split scaffolds into contigs -my $genome_size; # estimated or known genome size (will be used for some stats) - -GetOptions ("limit=i" => \$limit, - "csv" => \$csv, - "graph" => \$graph, - "n=i" => \$n_limit, - "genome_size=i" => \$genome_size); - -# set defaults -$limit = 1000000000 if (!$limit); -$n_limit = 25 if (!$n_limit); - - -# check we have a suitable input file -my $usage = "Usage: assemblathon_stats.pl -options: - -limit limit analysis to first sequences (useful for testing) - -csv produce a CSV output file of all results - -graph produce a CSV output file of NG(X) values (NG1 through to NG99), suitable for graphing - -n specify how many consecutive N characters should be used to split scaffolds into contigs - -genome_size estimated or known genome size -"; - -die "$usage" unless (@ARGV == 1); -my ($file) = @ARGV; - -############################################### -# -# S o m e G l o b a l v a r i a b l e s -# -############################################### - -my $scaffolded_contigs = 0; # how many contigs that are part of scaffolds (sequences must have $n_limit consecutive Ns) -my $scaffolded_contig_length = 0; # total length of all scaffolded contigs -my $unscaffolded_contigs = 0; # how many 'orphan' contigs, not part of a scaffold -my $unscaffolded_contig_length = 0; # total length of all contigs not part of scaffold -my $w = 60; # formatting width for output -my %data; # data structure to hold all sequence info key is either 'scaffold', 'contig' or intermediate', values are seqs & length arrays -my (@results, @headers); # arrays to store results (for use with -csv option) - - - -# make first loop through file, capture some basic info and add sequences to arrays -process_FASTA($file); - -print "\n---------------- Information for assembly \'$file\' ----------------\n\n"; - -if(defined($genome_size)){ - my $mbp_size = sprintf("%.2f", $genome_size / 1000000); - printf "%${w}s %10s\n", "Assumed genome size (Mbp)", $mbp_size; -} - -# produce scaffold statistics -sequence_statistics('scaffold'); - -# produce a couple of intermediate statistics based on scaffolded contigs vs unscaffolded contigs -sequence_statistics('intermediate'); - -# finish with contig stats -sequence_statistics('contig'); - -# produce CSV output if required -write_csv($file) if ($csv); - -exit(0); - - - -########################################## -# -# -# S U B R O U T I N E S -# -# -########################################## - - -########################################## -# M A I N loop through FASTA file -########################################## - -sub process_FASTA{ - - my ($seqs) = @_; - - my $input; - - # if dealing with gzip file, treat differently - if($seqs =~ m/\.gz$/){ - open($input, "gunzip -c $seqs |") or die "Can't open a pipe to $seqs\n"; - } else{ - open($input, "<", "$seqs") or die "Can't open $seqs\n"; - } - - my $fasta = new FAlite_943e0fb(\*$input); - - # want to keep track of various contig + scaffold counts - my $seq_count = 0; - - while(my $entry = $fasta->nextEntry){ - my $seq = uc($entry->seq); - my $length = length($seq); - $seq_count++; - - # everything gets pushed to scaffolds array - push(@{$data{scaffold}{seqs}},$seq); - push(@{$data{scaffold}{lengths}},$length); - - # if there are not at least 25 consecutive Ns in the sequence we need to split it into contigs - # otherwise the sequence must be a contig itself and it still needs to be put in @contigs array - if ($seq =~ m/N{$n_limit}/){ - - # add length to $scaffolded_contig_length - $scaffolded_contig_length += $length; - - # loop through all contigs that comprise the scaffold - foreach my $contig (split(/N{$n_limit,}/, $seq)){ - next unless my $length = length($contig); - $scaffolded_contigs++; - push(@{$data{contig}{seqs}},$contig); - push(@{$data{contig}{lengths}},$length); - } - } else { - # must be here if the scaffold is actually just a contig (or is a scaffold with < $n_limit Ns) - $unscaffolded_contigs++; - $unscaffolded_contig_length += $length; - push(@{$data{contig}{seqs}},$seq); - push(@{$data{contig}{lengths}},$length); - } - # for testing, just use a few sequences - last if ($seq_count >= $limit); - - } - close($input); -} - - -########################################## -# Calculate basic assembly metrics -########################################## - -sub sequence_statistics{ - my ($type) = @_; - - print "\n"; - - # need descriptions of each result - my $desc; - - # there are just a couple of intermediate level statistics to print - if($type eq 'intermediate'){ - my $total_size = sum(@{$data{scaffold}{lengths}}); - - # now calculate percentage of assembly that is accounted for by scaffolded contigs - my $percent = sprintf("%.1f",($scaffolded_contig_length / $total_size) * 100); - $desc = "Percentage of assembly in scaffolded contigs"; - printf "%${w}s %10s\n", $desc, "$percent%"; - store_results($desc, $percent) if ($csv); - - # now calculate percentage of assembly that is accounted for by unscaffolded contigs - $percent = sprintf("%.1f",($unscaffolded_contig_length / $total_size) * 100); - $desc = "Percentage of assembly in unscaffolded contigs"; - printf "%${w}s %10s\n", $desc, "$percent%"; - store_results($desc, $percent) if ($csv); - - - # statistics that describe N regions that join contigs in scaffolds - - # get number of breaks - my $contig_count = scalar(@{$data{contig}{lengths}}); - my $scaffold_count = scalar(@{$data{scaffold}{lengths}}); - my $average_contigs_per_scaffold = sprintf("%.1f",$contig_count / $scaffold_count); - $desc = "Average number of contigs per scaffold"; - printf "%${w}s %10s\n", $desc, $average_contigs_per_scaffold; - store_results($desc, $average_contigs_per_scaffold) if ($csv); - - # now calculate average length of break between contigs - # just find all runs of Ns in scaffolds (>= $n_limit) and calculate average length - my @contig_breaks; - foreach my $scaffold (@{$data{scaffold}{seqs}}){ - while($scaffold =~ m/(N{$n_limit,})/g){ - push(@contig_breaks, length($1)); - } - } - # set break size to zero if there are no Ns in scaffolds - my $average_break_length; - - if(@contig_breaks == 0){ - $average_break_length = 0; - } else{ - $average_break_length = sum(@contig_breaks) / scalar(@contig_breaks); - } - if($n_limit == 1) { - $desc = "Mean length of breaks (>=${n_limit}N) between contigs in scaffold"; - } else { - $desc = "Mean length of breaks (>=${n_limit}Ns) between contigs in scaffold"; - } - if(length($n_limit)>=5) { - printf "%${w}s %9d\n", $desc, $average_break_length; - } else { - printf "%${w}s %10d\n", $desc, $average_break_length; - } - store_results($desc, $average_break_length) if ($csv); - return(); - } - - - # n - my $count = scalar(@{$data{$type}{lengths}}); - $desc = "Number of ${type}s"; - printf "%${w}s %10d\n", $desc, $count; - store_results($desc, $count) if ($csv); - - - - # more contig details (only for contigs) - if ($type eq 'contig'){ - $desc = "Number of contigs in scaffolds"; - printf "%${w}s %10d\n",$desc, $scaffolded_contigs; - store_results($desc, $scaffolded_contigs) if ($csv); - - $desc = "Number of contigs not in scaffolds"; - printf "%${w}s %10d\n", $desc,$unscaffolded_contigs; - store_results($desc, $unscaffolded_contigs) if ($csv); - } - - - # total size of sequences - my $total_size = sum(@{$data{$type}{lengths}}); - $desc = "Total size of ${type}s"; - printf "%${w}s %10d\n", $desc, $total_size; - store_results($desc, $total_size) if ($csv); - - - # For scaffold data only, can caluclate the percentage of known genome size - # and also the amount of useful sequence - if ($type eq 'scaffold' && defined($genome_size)){ - my $percent = sprintf("%.1f",($total_size / $genome_size) * 100); - $desc = "Total scaffold length as percentage of assumed genome size"; - printf "%${w}s %10s\n", $desc, "$percent%"; - store_results($desc, $percent) if ($csv); - - # Also want to find total fraction of genome (based on estimated size) that is - # in 'non-useful scaffolds', those below average size of vertebrate gene - # (taken to be 25 kbp) - my $useful_length = 25000; - my $sum_useful = 0; - foreach my $length (@{$data{$type}{lengths}}){ - ($sum_useful += $length) if ($length >= $useful_length); - } - # calculate how much non-useful sequence there was - $desc = "Useful amount of $type sequences (>= 25K nt)"; - printf "%${w}s %10d\n", $desc, $sum_useful; - store_results($desc, $sum_useful) if ($csv); - - my $percent_useful = sprintf("%.1f",($sum_useful / $genome_size) * 100); - $desc = "% of estimated genome that is useful"; - printf "%${w}s %10s\n", $desc, "$percent_useful%"; - store_results($desc, $percent_useful) if ($csv); - - } - - - # longest and shortest sequences - my $max = max(@{$data{$type}{lengths}}); - $desc = "Longest $type"; - printf "%${w}s %10d\n", $desc, $max; - store_results($desc, $max) if ($csv); - - my $min = min(@{$data{$type}{lengths}}); - $desc = "Shortest $type"; - printf "%${w}s %10d\n", $desc, $min; - store_results($desc, $min) if ($csv); - - - # find number of sequences above certain sizes - my %sizes_to_shorthand = (1000 => '1K', - 10000 => '10K', - 100000 => '100K', - 1000000 => '1M', - 10000000 => '10M'); - - foreach my $size (qw(1000 10000 100000 1000000 10000000)){ - my $matches = grep { $_ > $size } @{$data{$type}{lengths}}; - my $percent = sprintf("%.1f", ($matches / $count) * 100); - - $desc = "Number of ${type}s > $sizes_to_shorthand{$size} nt"; - printf "%${w}s %10d %5s%%\n", $desc, $matches, $percent; - store_results($desc, $matches) if ($csv); - - $desc = "Percentage of ${type}s > $sizes_to_shorthand{$size} nt"; - store_results($desc, $percent) if ($csv); - } - - - # mean sequence size - my $mean = sprintf("%.0f",$total_size / $count); - $desc = "Mean $type size"; - printf "%${w}s %10d\n", $desc, $mean; - store_results($desc, $mean) if ($csv); - - # median sequence size - my $median = (sort{$a <=> $b} @{$data{$type}{lengths}})[$count/2]; - $desc = "Median $type size"; - printf "%${w}s %10d\n", $desc, $median; - store_results($desc, $median) if ($csv); - - - - ################################################################################## - # - # N50 values - # - # Includes N(x) values, NG(x) (using assumed genome size) - # and L(x) values (number of sequences larger than or equal to N50 sequence size) - ################################################################################## - - # keep track of cumulative assembly size (starting from smallest seq) - my $running_total = 0; - - # want to store all N50-style values from N1..N100. First target size to pass is N1 - my $n_index = 1; - my @n_values; - my $n50_length = 0; - - my $i = 0; - - my $x = $total_size * 0.5; - # start with longest lengths scaffold/contig - foreach my $length (reverse sort{$a <=> $b} @{$data{$type}{lengths}}){ - $i++; - $running_total += $length; - - # check the current sequence and all sequences shorter than current one - # to see if they exceed the current NX value - while($running_total > int (($n_index / 100) * $total_size)){ - if ($n_index == 50){ - $n50_length = $length; - $desc = "N50 $type length"; - printf "%${w}s %10d\n", $desc, $length; - store_results($desc, $length) if ($csv); - - # L50 = number of scaffolds/contigs that are longer than or equal to the N50 size - $desc = "L50 $type count"; - printf "%${w}s %10d\n","L50 $type count", $i; - store_results($desc, $i) if ($csv); - } - $n_values[$n_index] = $length; - $n_index++; - } - } - - my @ng_values; - - # do we have an estimated/known genome size to work with? - if(defined($genome_size)){ - my $ng_index = 1; - my $ng50_length = 0; - - $running_total = 0; - $i = 0; - - foreach my $length (reverse sort{$a <=> $b} @{$data{$type}{lengths}}){ - $i++; - $running_total += $length; - - # now do the same for NG values, using assumed genome size - while($running_total > int (($ng_index / 100) * $genome_size)){ - if ($ng_index == 50){ - $ng50_length = $length; - $desc = "NG50 $type length"; - printf "%${w}s %10d\n", $desc, $length; - store_results($desc, $length) if ($csv); - - $desc = "LG50 $type count"; - printf "%${w}s %10d\n", $desc, $i; - store_results($desc, $i) if ($csv); - } - $ng_values[$ng_index] = $length; - $ng_index++; - } - } - - # calculate N50/NG50 difference - my $n50_diff = abs($ng50_length - $n50_length); - $desc = "N50 $type - NG50 $type length difference"; - printf "%${w}s %10d\n", $desc, $n50_diff; - store_results($desc, $n50_diff) if ($csv); - - } - # add final value to @n_values and @ng_values which will just be the shortest sequence -# $n_values[100] = $min; -# $ng_values[100] = $min; - - - # base frequencies - my %bases; - - my $seq = join('',@{$data{$type}{seqs}}); - my $length = length($seq); - - # count mononucleotide frequencies - $bases{A} = ($seq =~ tr/A/A/); - $bases{C} = ($seq =~ tr/C/C/); - $bases{G} = ($seq =~ tr/G/G/); - $bases{T} = ($seq =~ tr/T/T/); - $bases{N} = ($seq =~ tr/N/N/); - - my $base_count = 0; - foreach my $base (qw(A C G T N)){ - my $percent = sprintf("%.2f", ($bases{$base} / $length) * 100); - $desc = "$type %$base"; - printf "%${w}s %10s\n", $desc, $percent; - store_results($desc, $percent) if ($csv); - $base_count += $bases{$base}; - } - - # calculate remainder ('other) in case there are other characters present - my $other = $length - $base_count; - my $percent = sprintf("%.2f", ($other / $length) * 100); - $desc = "$type %non-ACGTN"; - printf "%${w}s %10s\n",$desc, $percent; - store_results($desc, $percent) if ($csv); - - $desc = "Number of $type non-ACGTN nt"; - printf "%${w}s %10d\n",$desc, $other; - store_results($desc, $other) if ($csv); - - - # anything to dump for graphing? - if($graph){ - - # create new output file name - my $file_name = $file; - $file_name =~ s/\.gz$//; - $file_name =~ s/\.(fa|fasta)$//; - $file_name .= ".${type}.NG50.csv"; - - open(my $out, ">", "$file_name") or die "Can't create $file_name\n"; - print $out join (',',"Assembly",1..99), "\n"; - - # make some guesses of what might constitute the unique assembly ID - my $assembly_ID = $file; - ($assembly_ID) = $file =~ m/^([A-Z]\d{1,2})_/ if ($file =~ m/^[A-Z]\d{1,2}_/); - ($assembly_ID) = $file =~ m/^((bird|snake|fish)_\d+(C|E))_/ if ($file =~ m/^(bird|snake|fish)_\d+C|E_/); - - # CSV file, with filename in first column - print $out "$assembly_ID"; - - for (my $i = 1; $i < 100; $i++){ - # higher NG values might not be present if assembly is poor - if (defined $ng_values[$i]){ - print $out ",$ng_values[$i]"; - } else{ - print $out ",0"; - } - } - print $out "\n"; - close($out); - } -} - -# simple routine to add results to a pair of arrays that will be used for printing results later on -# if -csv option is used -sub store_results{ - my ($desc, $result) = @_; - - push(@headers,$desc); - push(@results,$result); -} - -sub write_csv{ - my ($file) = @_; - - # create new output file name - my $output = $file; - $output =~ s/\.gz$//; - $output =~ s/\.(fa|fasta)$//; - $output .= ".csv"; - - # make some guesses of what might constitute the unique assembly ID - my $assembly_ID = $file; - ($assembly_ID) = $file =~ m/^([A-Z]\d{1,2})_/ if ($file =~ m/^[A-Z]\d{1,2}_/); - ($assembly_ID) = $file =~ m/^((bird|snake|fish)_\d+(C|E))_/ if ($file =~ m/^(bird|snake|fish)_\d+C|E_/); - - open(my $out, ">", $output) or die "Can't create $output\n"; - - print $out "Assembly,"; - foreach my $header (@headers){ - print $out "$header,"; - } - print $out "\n"; - - print $out "$assembly_ID,"; - foreach my $result (@results){ - print $out "$result,"; - } - print $out "\n"; - - - close($out); -} diff --git a/bin/assembly_2_bedpe_943e0fb.py b/bin/assembly_2_bedpe_943e0fb.py deleted file mode 100755 index 94e49681..00000000 --- a/bin/assembly_2_bedpe_943e0fb.py +++ /dev/null @@ -1,47 +0,0 @@ -#!/usr/bin/env python - -import sys -import pandas as pd - - -def read_assembly_file_cols(assembly_file_name): - with open(assembly_file_name, "r") as file: - lines = file.readlines() - - list_of_items = [line.replace("\n", "").split(" ") for line in lines] - list_of_three_tuples = [items for items in list_of_items if len(items) == 3] - list_of_three_tuples_wt = [ - [x[0], int(x[1]), int(x[2])] for x in list_of_three_tuples - ] - - df = pd.DataFrame(list_of_three_tuples_wt) - df.columns = ["name", "number", "length"] - - return df - - -def make_bedpe_cols(assembly_file_pd): - pd = assembly_file_pd - pd["cum_length"] = pd["length"].cumsum() - pd["end_index"] = pd["cum_length"] - 1 - - start_index = pd["end_index"].shift(periods=1, fill_value=-1) + 1 - pd["start_index"] = start_index - - return pd - - -def print_bed_pe_file(bed_pe_df): - df = bed_pe_df - print("chr1\tx1\tx2\tchr2\ty1\ty2\tname\tscore\tstrand1\tstrand2\tcolor") - for index, row in df.iterrows(): - print( - f"assembly\t{row['start_index']}\t{row['end_index']}\tassembly\t{row['start_index']}\t{row['end_index']}\t{row['name'].replace('>', '')}\t.\t.\t.\t0,0,255" - ) - - -if __name__ == "__main__": - assembly_file_name = sys.argv[1] - - assembly_file_cols = read_assembly_file_cols(assembly_file_name) - print_bed_pe_file(make_bedpe_cols(assembly_file_cols)) diff --git a/bin/assembly_qc_report_943e0fb.py b/bin/assembly_qc_report_943e0fb.py deleted file mode 100755 index f305101e..00000000 --- a/bin/assembly_qc_report_943e0fb.py +++ /dev/null @@ -1,67 +0,0 @@ -#!/usr/bin/env python3.9 - -import json -import yaml - -from report_modules.report_printer import ReportPrinter - -from report_modules.parsers.params_parser import parse_params_json -from report_modules.parsers.tools_parser import parse_tools_yaml - -from report_modules.parsers.gff3_validate_parser import parse_gff3_validate_folder -from report_modules.parsers.fasta_validate_parser import parse_fasta_validate_folder - -from report_modules.parsers.ncbi_fcs_adaptor_parser import parse_ncbi_fcs_adaptor_folder -from report_modules.parsers.ncbi_fcs_gx_parser import parse_ncbi_fcs_gx_folder -from report_modules.parsers.assemblathon_stats_parser import ( - parse_assemblathon_stats_folder, -) -from report_modules.parsers.genometools_gt_stat_parser import ( - parse_genometools_gt_stat_folder, -) -from report_modules.parsers.busco_parser import parse_busco_folder -from report_modules.parsers.tidk_parser import parse_tidk_folder -from report_modules.parsers.lai_parser import parse_lai_folder -from report_modules.parsers.kraken2_parser import parse_kraken2_folder -from report_modules.parsers.hic_parser import parse_hic_folder -from report_modules.parsers.circos_parser import parse_circos_folder - -if __name__ == "__main__": - params_dict, params_table = parse_params_json() - tools_dict, tools_table = parse_tools_yaml() - - data_from_tools = {} - - data_from_tools = {**data_from_tools, **parse_gff3_validate_folder()} - data_from_tools = {**data_from_tools, **parse_fasta_validate_folder()} - data_from_tools = {**data_from_tools, **parse_ncbi_fcs_adaptor_folder()} - data_from_tools = {**data_from_tools, **parse_ncbi_fcs_gx_folder()} - data_from_tools = {**data_from_tools, **parse_assemblathon_stats_folder()} - data_from_tools = {**data_from_tools, **parse_genometools_gt_stat_folder()} - data_from_tools = {**data_from_tools, **parse_busco_folder()} - data_from_tools = {**data_from_tools, **parse_tidk_folder()} - data_from_tools = {**data_from_tools, **parse_lai_folder()} - data_from_tools = {**data_from_tools, **parse_kraken2_folder()} - data_from_tools = {**data_from_tools, **parse_hic_folder()} - data_from_tools = {**data_from_tools, **parse_circos_folder()} - - with open("software_versions.yml", "r") as f: - versions_from_ch_versions = yaml.safe_load(f) - - data_from_tools = { - "PARAMS_DICT": params_dict, - "PARAMS_TABLE": params_table, - "TOOLS_DICT": tools_dict, - "TOOLS_TABLE": tools_table, - "VERSIONS": { - **versions_from_ch_versions, - "JUICEBOX_JS": "2.4.3", - }, - **data_from_tools, - } - - report_printer = ReportPrinter() - report_template = report_printer.print_template(data_from_tools) - - with open("report.json", "w") as fp: - json.dump(data_from_tools, fp) diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py new file mode 100755 index 00000000..4a758fe0 --- /dev/null +++ b/bin/check_samplesheet.py @@ -0,0 +1,259 @@ +#!/usr/bin/env python + + +"""Provide a command line tool to validate and transform tabular samplesheets.""" + + +import argparse +import csv +import logging +import sys +from collections import Counter +from pathlib import Path + +logger = logging.getLogger() + + +class RowChecker: + """ + Define a service that can validate and transform each given row. + + Attributes: + modified (list): A list of dicts, where each dict corresponds to a previously + validated and transformed row. The order of rows is maintained. + + """ + + VALID_FORMATS = ( + ".fq.gz", + ".fastq.gz", + ) + + def __init__( + self, + sample_col="sample", + first_col="fastq_1", + second_col="fastq_2", + single_col="single_end", + **kwargs, + ): + """ + Initialize the row checker with the expected column names. + + Args: + sample_col (str): The name of the column that contains the sample name + (default "sample"). + first_col (str): The name of the column that contains the first (or only) + FASTQ file path (default "fastq_1"). + second_col (str): The name of the column that contains the second (if any) + FASTQ file path (default "fastq_2"). + single_col (str): The name of the new column that will be inserted and + records whether the sample contains single- or paired-end sequencing + reads (default "single_end"). + + """ + super().__init__(**kwargs) + self._sample_col = sample_col + self._first_col = first_col + self._second_col = second_col + self._single_col = single_col + self._seen = set() + self.modified = [] + + def validate_and_transform(self, row): + """ + Perform all validations on the given row and insert the read pairing status. + + Args: + row (dict): A mapping from column headers (keys) to elements of that row + (values). + + """ + self._validate_sample(row) + self._validate_first(row) + self._validate_second(row) + self._validate_pair(row) + self._seen.add((row[self._sample_col], row[self._first_col])) + self.modified.append(row) + + def _validate_sample(self, row): + """Assert that the sample name exists and convert spaces to underscores.""" + if len(row[self._sample_col]) <= 0: + raise AssertionError("Sample input is required.") + # Sanitize samples slightly. + row[self._sample_col] = row[self._sample_col].replace(" ", "_") + + def _validate_first(self, row): + """Assert that the first FASTQ entry is non-empty and has the right format.""" + if len(row[self._first_col]) <= 0: + raise AssertionError("At least the first FASTQ file is required.") + self._validate_fastq_format(row[self._first_col]) + + def _validate_second(self, row): + """Assert that the second FASTQ entry has the right format if it exists.""" + if len(row[self._second_col]) > 0: + self._validate_fastq_format(row[self._second_col]) + + def _validate_pair(self, row): + """Assert that read pairs have the same file extension. Report pair status.""" + if row[self._first_col] and row[self._second_col]: + row[self._single_col] = False + first_col_suffix = Path(row[self._first_col]).suffixes[-2:] + second_col_suffix = Path(row[self._second_col]).suffixes[-2:] + if first_col_suffix != second_col_suffix: + raise AssertionError("FASTQ pairs must have the same file extensions.") + else: + row[self._single_col] = True + + def _validate_fastq_format(self, filename): + """Assert that a given filename has one of the expected FASTQ extensions.""" + if not any(filename.endswith(extension) for extension in self.VALID_FORMATS): + raise AssertionError( + f"The FASTQ file has an unrecognized extension: {filename}\n" + f"It should be one of: {', '.join(self.VALID_FORMATS)}" + ) + + def validate_unique_samples(self): + """ + Assert that the combination of sample name and FASTQ filename is unique. + + In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the + number of times the same sample exist, but with different FASTQ files, e.g., multiple runs per experiment. + + """ + if len(self._seen) != len(self.modified): + raise AssertionError("The pair of sample name and FASTQ must be unique.") + seen = Counter() + for row in self.modified: + sample = row[self._sample_col] + seen[sample] += 1 + row[self._sample_col] = f"{sample}_T{seen[sample]}" + + +def read_head(handle, num_lines=10): + """Read the specified number of lines from the current position in the file.""" + lines = [] + for idx, line in enumerate(handle): + if idx == num_lines: + break + lines.append(line) + return "".join(lines) + + +def sniff_format(handle): + """ + Detect the tabular format. + + Args: + handle (text file): A handle to a `text file`_ object. The read position is + expected to be at the beginning (index 0). + + Returns: + csv.Dialect: The detected tabular format. + + .. _text file: + https://docs.python.org/3/glossary.html#term-text-file + + """ + peek = read_head(handle) + handle.seek(0) + sniffer = csv.Sniffer() + dialect = sniffer.sniff(peek) + return dialect + + +def check_samplesheet(file_in, file_out): + """ + Check that the tabular samplesheet has the structure expected by nf-core pipelines. + + Validate the general shape of the table, expected columns, and each row. Also add + an additional column which records whether one or two FASTQ reads were found. + + Args: + file_in (pathlib.Path): The given tabular samplesheet. The format can be either + CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``. + file_out (pathlib.Path): Where the validated and transformed samplesheet should + be created; always in CSV format. + + Example: + This function checks that the samplesheet follows the following structure, + see also the `viral recon samplesheet`_:: + + sample,fastq_1,fastq_2 + SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz + SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz + SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, + + .. _viral recon samplesheet: + https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv + + """ + required_columns = {"sample", "fastq_1", "fastq_2"} + # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. + with file_in.open(newline="") as in_handle: + reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle)) + # Validate the existence of the expected header columns. + if not required_columns.issubset(reader.fieldnames): + req_cols = ", ".join(required_columns) + logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.") + sys.exit(1) + # Validate each row. + checker = RowChecker() + for i, row in enumerate(reader): + try: + checker.validate_and_transform(row) + except AssertionError as error: + logger.critical(f"{str(error)} On line {i + 2}.") + sys.exit(1) + checker.validate_unique_samples() + header = list(reader.fieldnames) + header.insert(1, "single_end") + # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. + with file_out.open(mode="w", newline="") as out_handle: + writer = csv.DictWriter(out_handle, header, delimiter=",") + writer.writeheader() + for row in checker.modified: + writer.writerow(row) + + +def parse_args(argv=None): + """Define and immediately parse command line arguments.""" + parser = argparse.ArgumentParser( + description="Validate and transform a tabular samplesheet.", + epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv", + ) + parser.add_argument( + "file_in", + metavar="FILE_IN", + type=Path, + help="Tabular input samplesheet in CSV or TSV format.", + ) + parser.add_argument( + "file_out", + metavar="FILE_OUT", + type=Path, + help="Transformed output samplesheet in CSV format.", + ) + parser.add_argument( + "-l", + "--log-level", + help="The desired log level (default WARNING).", + choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"), + default="WARNING", + ) + return parser.parse_args(argv) + + +def main(argv=None): + """Coordinate argument parsing and program execution.""" + args = parse_args(argv) + logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s") + if not args.file_in.is_file(): + logger.error(f"The given input file {args.file_in} was not found!") + sys.exit(2) + args.file_out.parent.mkdir(parents=True, exist_ok=True) + check_samplesheet(args.file_in, args.file_out) + + +if __name__ == "__main__": + sys.exit(main()) diff --git a/bin/color_circos_bundles_by_contig_943e0fb.py b/bin/color_circos_bundles_by_contig_943e0fb.py deleted file mode 100755 index d501323c..00000000 --- a/bin/color_circos_bundles_by_contig_943e0fb.py +++ /dev/null @@ -1,77 +0,0 @@ -#!/usr/bin/env python - -import sys -import re - -bundled_links_file_name = sys.argv[1] - - -def natural_key(string): - """Return a list of keys that sort naturally.""" - return [int(s) if s.isdigit() else s for s in re.split(r"(\d+)", string)] - - -def hsv2rgb(h, s, v): - """Convert HSV color to RGB color.""" - h = float(h) - s = float(s) - v = float(v) - h60 = h / 60.0 - h60f = int(h60) - hi = int(h60f) % 6 - f = h60 - h60f - p = v * (1 - s) - q = v * (1 - f * s) - t = v * (1 - (1 - f) * s) - r, g, b = 0, 0, 0 - if hi == 0: - r, g, b = v, t, p - elif hi == 1: - r, g, b = q, v, p - elif hi == 2: - r, g, b = p, v, t - elif hi == 3: - r, g, b = p, q, v - elif hi == 4: - r, g, b = t, p, v - elif hi == 5: - r, g, b = v, p, q - return int(r * 255), int(g * 255), int(b * 255) - - -def generate_colors(num_colors): - """Generate a list of colors""" - hue_step = int(360 / num_colors) - hue = 0 - colors = [] - for i in range(num_colors): - red, green, blue = hsv2rgb(hue, 0.8, 0.8) - colors.append(f"{red},{green},{blue},0.5") - hue += hue_step - return colors - - -def read_file_lines(file_path): - with open(file_path, "r") as f: - return f.readlines() - - -def generate_colors_by_ids(bundle_file_lines): - """Create a dictionary to map unique target ids to colors""" - unique_ids = set( - line.split()[3] for line in bundle_file_lines - ) # index 3: Target ids - num_unique_ids = len(unique_ids) - colors = generate_colors(num_unique_ids) - return dict(zip(sorted(unique_ids, key=natural_key), colors)) - - -if __name__ == "__main__": - bundle_file_lines = read_file_lines(bundled_links_file_name) - id_to_color = generate_colors_by_ids(bundle_file_lines) - - for line in bundle_file_lines: - parts = line.strip().split() - unique_id = parts[3] # index 3: Target ids - color = id_to_color[unique_id] - print(" ".join(parts[0:6] + [f"color=({color})", parts[6]])) diff --git a/bin/count_t2t_complete_scaffolds_6fdcd87.py b/bin/count_t2t_complete_scaffolds_6fdcd87.py deleted file mode 100755 index d8810b68..00000000 --- a/bin/count_t2t_complete_scaffolds_6fdcd87.py +++ /dev/null @@ -1,57 +0,0 @@ -#!/usr/bin/env python - -import pandas as pd -import sys - - -def get_combined_repeat_number(data): - data["combined_repeat_number"] = ( - data["forward_repeat_number"] + data["reverse_repeat_number"] - ) - return data - - -def check_edges(data): - largest_indices = data["combined_repeat_number"].nlargest(2).index - - if len(largest_indices) < 2: - return data["id"].iloc[0], False, data["window"].max() - - if largest_indices[0] == data.index[0] and largest_indices[1] == data.index[-1]: - return data["id"].iloc[0], True, data["window"].max() - elif largest_indices[0] == data.index[-1] and largest_indices[1] == data.index[0]: - return data["id"].iloc[0], True, data["window"].max() - else: - return data["id"].iloc[0], False, data["window"].max() - - -def count_t2t_complete_scaffolds(tidk_tsv_file_path): - tidk_tsv_as_pd = pd.read_csv(tidk_tsv_file_path, sep="\t") - - grouped_data = tidk_tsv_as_pd.groupby("id") - ids_with_checks_lens = [] - for _, group in grouped_data: - group = get_combined_repeat_number(group) - - ids_with_checks_lens.append(check_edges(group)) - - count_MB = sum( - [ - 1 if check and length > 1000_000 else 0 - for (_, check, length) in ids_with_checks_lens - ] - ) - count_KB = sum( - [ - 1 if check and length > 1000 else 0 - for (_, check, length) in ids_with_checks_lens - ] - ) - print( - f"Number of T2T complete scaffolds: {count_MB} (> 1 Mbp), {count_KB} (> 1 Kbp)" - ) - - -if __name__ == "__main__": - tidk_tsv_file_path = sys.argv[1] - count_t2t_complete_scaffolds(tidk_tsv_file_path) diff --git a/bin/hic_2_html_fc62f04.py b/bin/hic_2_html_fc62f04.py deleted file mode 100755 index eacc08f8..00000000 --- a/bin/hic_2_html_fc62f04.py +++ /dev/null @@ -1,24 +0,0 @@ -#!/usr/bin/env python - -import sys -from pathlib import Path -import os - - -if __name__ == "__main__": - hic_file_name = os.path.basename(sys.argv[1]) - - projectDir = "/".join(__file__.split("/")[0:-1]) - html_template_path = Path( - f"{projectDir}/report_modules/templates/hic/hic_html_template.html" - ) - - with open(html_template_path) as f: - html_file_lines = "".join(f.readlines()) - - filled_template = html_file_lines.replace("HIC_FILE_NAME", hic_file_name).replace( - "BEDPE_FILE_NAME", - f"{hic_file_name.replace('.hic', '')}.assembly.bedpe", - ) - - print(filled_template) diff --git a/bin/report_modules/parsers/assemblathon_stats_parser.py b/bin/report_modules/parsers/assemblathon_stats_parser.py deleted file mode 100644 index 8f124bf7..00000000 --- a/bin/report_modules/parsers/assemblathon_stats_parser.py +++ /dev/null @@ -1,53 +0,0 @@ -import os -from pathlib import Path -import pandas as pd -from tabulate import tabulate -import re - -from report_modules.parsers.parsing_commons import sort_list_of_results - - -def parse_assemblathon_stats_folder(folder_name="assemblathon_stats"): - dir = os.getcwdb().decode() - reports_folder_path = Path(f"{dir}/{folder_name}") - - if not os.path.exists(reports_folder_path): - return {} - - list_of_report_files = reports_folder_path.glob("*.csv") - - data = {"ASSEMBLATHON_STATS": []} - - for report_path in list_of_report_files: - report_table = pd.read_csv(report_path) - report_table.drop( - list(report_table.filter(regex="^Unnamed:")), axis=1, inplace=True - ) - - stat_names = report_table.columns.values.tolist() - stat_values = report_table.iloc[0].values.tolist() - - report_table_t = pd.DataFrame({"Stat": stat_names, "Value": stat_values}) - - file_tokens = re.findall( - r"([\w]+)_stats.csv", - os.path.basename(str(report_path)), - )[0] - - data["ASSEMBLATHON_STATS"].append( - { - "hap": file_tokens, - "report_table": report_table.to_dict("records"), - "report_table_html": tabulate( - report_table_t, - headers=["Stat", "Value"], - tablefmt="html", - numalign="left", - showindex=False, - ), - } - ) - - return { - "ASSEMBLATHON_STATS": sort_list_of_results(data["ASSEMBLATHON_STATS"], "hap") - } diff --git a/bin/report_modules/parsers/busco_parser.py b/bin/report_modules/parsers/busco_parser.py deleted file mode 100644 index 22dcff27..00000000 --- a/bin/report_modules/parsers/busco_parser.py +++ /dev/null @@ -1,197 +0,0 @@ -import pandas as pd -from tabulate import tabulate -import re -import os -import re -import base64 -from pathlib import Path - -from report_modules.parsers.parsing_commons import sort_list_of_results - - -class BuscoParser: - def __init__(self, file_data): - self.file_data = file_data - self.stats_dict = {} - - def parse_report(self): - self.stats_dict["version"] = self.get_busco_version(self.file_data) - self.stats_dict["lineage"] = self.get_lineage_dataset(self.file_data) - self.stats_dict["created"] = self.get_creation_date(self.file_data) - self.stats_dict["mode"] = self.get_run_mode(self.file_data) - self.stats_dict["predictor"] = self.get_gene_predictor(self.file_data) - self.stats_dict["search_percentages"] = self.get_busco_percentages( - self.file_data - ) - self.stats_dict["dependencies"] = self.get_deps_and_versions(self.file_data) - self.stats_dict["results_table"] = self.get_busco_result_table(self.file_data) - - # include busco results dictionary for use in json dump - self.stats_dict["results_dict"] = self.get_busco_result_dict(self.file_data) - # include dependencies dictionary for use in json dump - self.stats_dict["dependencies_dict"] = self.get_deps_and_versions_dict( - self.file_data - ) - - return self.stats_dict - - def get_busco_version(self, data): - p = re.compile("BUSCO version is: (.*)") - result = p.search(data).group(1).strip() - return result - - def get_lineage_dataset(self, data): - p = re.compile("The lineage dataset is: (.*)") - result = p.search(data).group(1).split()[0] - return result - - def get_creation_date(self, data): - p = re.compile("The lineage dataset is: (.*)") - result = p.search(data) - result = result.group(1).split()[3][:-1] - return result - - def get_run_mode(self, data): - p = re.compile("BUSCO was run in mode: (.*)") - result = p.search(data).group(1) - return result - - def get_gene_predictor(self, data): - p = re.compile("Gene predictor used: (.*)") - gene_predictor = p.search(data) - - if gene_predictor == None: - return "None" - - result = gene_predictor.group(1) - q = re.compile(f"{gene_predictor.group(1)}: (.*)") - predictor_version = q.search(data) - return result - - def get_busco_percentages(self, data): - p = re.compile("C:(.*)") - result = p.search(data).group(0).strip() - return result - - def get_deps_and_versions(self, file_data): - list_of_lines = file_data.split("\n") - for index, line in enumerate(list_of_lines): - if "Dependencies and versions" in line: - all_deps = ( - "".join(list_of_lines[max(0, index + 1) : len(list_of_lines) - 2]) - .replace("\t", "\n") - .strip() - ) - - dep_dict = {} - for dep in all_deps.splitlines(): - dependency = dep.split(":")[0] - version = dep.split(":")[1].strip() - dep_dict[f"{dependency}"] = f"{version}" - df = pd.DataFrame(dep_dict.items(), columns=["Dependency", "Version"]) - - col_names = ["Dependency", "Version"] - table = tabulate( - df, headers=col_names, tablefmt="html", numalign="left", showindex=False - ) - return table - - # get dependencies dictionary instead of table to use in json dump - def get_deps_and_versions_dict(self, file_data): - list_of_lines = file_data.split("\n") - for index, line in enumerate(list_of_lines): - if "Dependencies and versions" in line: - all_deps = ( - "".join(list_of_lines[max(0, index + 1) : len(list_of_lines) - 2]) - .replace("\t", "\n") - .strip() - ) - - dep_dict = {} - for dep in all_deps.splitlines(): - dependency = dep.split(":")[0] - version = dep.split(":")[1].strip() - dep_dict[f"{dependency}"] = f"{version}" - - return dep_dict - - def get_busco_result_table(self, file_data): - list_of_lines = file_data.split("\n") - for index, line in enumerate(list_of_lines): - if "Dependencies and versions" in line: - dev_dep_index = index - - results_dict = {} - for index, line in enumerate(list_of_lines): - if "C:" in line: - for i in range(index + 1, dev_dep_index - 1): - number = list_of_lines[i].split("\t")[1] - descr = list_of_lines[i].split("\t")[2] - - results_dict[f"{descr}"] = f"{number}" - df = pd.DataFrame(results_dict.items(), columns=["Event", "Frequency"]) - col_names = ["Event", "Frequency"] - table = tabulate( - df, headers=col_names, tablefmt="html", numalign="left", showindex=False - ) - return table - - # get results dictionary instead of table to use in json dump - def get_busco_result_dict(self, file_data): - list_of_lines = file_data.split("\n") - for index, line in enumerate(list_of_lines): - if "Dependencies and versions" in line: - dev_dep_index = index - - results_dict = {} - for index, line in enumerate(list_of_lines): - if "C:" in line: - for i in range(index + 1, dev_dep_index - 1): - number = list_of_lines[i].split("\t")[1] - descr = list_of_lines[i].split("\t")[2] - - results_dict[f"{descr}"] = f"{number}" - - return results_dict - - -def parse_busco_folder(folder_name="busco_outputs"): - dir = os.getcwdb().decode() - busco_folder_path = Path(f"{dir}/{folder_name}") - - if not os.path.exists(busco_folder_path): - return {} - - list_of_files = busco_folder_path.glob("*.txt") - - plot_path = next(busco_folder_path.glob("*.png")) - - binary_fc = open(plot_path, "rb").read() - base64_utf8_str = base64.b64encode(binary_fc).decode("utf-8") - ext = str(plot_path).split(".")[-1] - busco_plot_url = f"data:image/{ext};base64,{base64_utf8_str}" - - data = {"BUSCO": []} - - for file in list_of_files: - file_data = "" - with open(file, "r") as file: - lines = file.readlines() - for line in lines: - file_data += line - parser = BuscoParser(file_data) - file_tokens = re.findall( - r"short_summary.specific.([\w]+).([\w]+)_([a-zA-Z0-9]+).txt", - os.path.basename(str(file)), - )[0] - stats = { - "hap": file_tokens[1], - "lineage": file_tokens[0], - **parser.parse_report(), - } - data["BUSCO"].append(stats) - - data["BUSCO"] = sort_list_of_results(data["BUSCO"], "hap") - data["BUSCO"][0]["busco_plot"] = busco_plot_url - - return data diff --git a/bin/report_modules/parsers/circos_parser.py b/bin/report_modules/parsers/circos_parser.py deleted file mode 100644 index 0df2d037..00000000 --- a/bin/report_modules/parsers/circos_parser.py +++ /dev/null @@ -1,52 +0,0 @@ -import os -from pathlib import Path -import base64 -import re - -from report_modules.parsers.parsing_commons import sort_list_of_results - - -def parse_circos_folder(folder_name="circos_outputs"): - dir = os.getcwdb().decode() - circos_folder_path = Path(f"{dir}/{folder_name}") - - if not os.path.exists(circos_folder_path): - return {} - - list_of_plot_files = [item for item in circos_folder_path.glob("*.png")] - - data = {"CIRCOS": []} - - for plot_path in list_of_plot_files: - file_tokens = re.findall( - r"([\w]+).on.([\w]+).([\w]+).png", - os.path.basename(str(plot_path)), - )[0] - - if os.path.getsize(plot_path) == 0: - data["CIRCOS"].append( - { - "tag.on.tag": f"{file_tokens[0]} : {file_tokens[1]} : {file_tokens[2]}", - "circos_plot": "", - "is_plot_empty": True, - } - ) - continue - - binary_fc = open(plot_path, "rb").read() - base64_utf8_str = base64.b64encode(binary_fc).decode("utf-8") - ext = str(plot_path).split(".")[-1] - plot_url = f"data:image/{ext}+xml;base64,{base64_utf8_str}" - - data["CIRCOS"].append( - { - "tag.on.tag": f"{file_tokens[0]} : {file_tokens[1]} : {file_tokens[2]}", - "circos_plot": plot_url, - "is_plot_empty": False, - } - ) - - if len(data["CIRCOS"]) < 1: - return {} - - return {"CIRCOS": sort_list_of_results(data["CIRCOS"], "tag.on.tag")} diff --git a/bin/report_modules/parsers/fasta_validate_parser.py b/bin/report_modules/parsers/fasta_validate_parser.py deleted file mode 100644 index 786978ec..00000000 --- a/bin/report_modules/parsers/fasta_validate_parser.py +++ /dev/null @@ -1,35 +0,0 @@ -import os -from pathlib import Path -import re - -from report_modules.parsers.parsing_commons import sort_list_of_results - - -def parse_fasta_validate_folder(folder_name="fastavalidator_logs"): - dir = os.getcwdb().decode() - logs_folder_path = Path(f"{dir}/{folder_name}") - - if not os.path.exists(logs_folder_path): - return {} - - list_of_log_files = logs_folder_path.glob("*.log") - - data = {"FASTA_VALIDATE": []} - - for log_path in list_of_log_files: - with open(log_path, "r") as f: - log_lines = [f"

{l}

" for l in f.readlines()] - - file_tokens = re.findall( - r"([\w]+).error.log", - os.path.basename(str(log_path)), - )[0] - - data["FASTA_VALIDATE"].append( - { - "hap": file_tokens, - "validation_log": "".join(log_lines), - } - ) - - return {"FASTA_VALIDATE": sort_list_of_results(data["FASTA_VALIDATE"], "hap")} diff --git a/bin/report_modules/parsers/genometools_gt_stat_parser.py b/bin/report_modules/parsers/genometools_gt_stat_parser.py deleted file mode 100644 index a4e4e6f4..00000000 --- a/bin/report_modules/parsers/genometools_gt_stat_parser.py +++ /dev/null @@ -1,344 +0,0 @@ -import os -from pathlib import Path -import pandas as pd -from tabulate import tabulate -import re -import matplotlib.pyplot as plt -import numpy as np -import base64 - -from report_modules.parsers.parsing_commons import sort_list_of_results - - -def parse_genometools_gt_stat_folder(folder_name="genometools_gt_stat"): - dir = os.getcwdb().decode() - reports_folder_path = Path(f"{dir}/{folder_name}") - - if not os.path.exists(reports_folder_path): - return {} - - list_of_report_files = reports_folder_path.glob("*.yml") - - data = {"GENOMETOOLS_GT_STAT": []} - - for report_path in list_of_report_files: - - NUM_GROUPS = -1 - ( - report_table_dict, - gene_length_distribution, - gene_score_distribution, - exon_length_distribution, - exon_number_distribution, - intron_length_distribution, - cds_length_distribution, - ) = extract_report_data(report_path, NUM_GROUPS) - - gene_length_distribution_graph = "" - if gene_length_distribution != []: - gene_length_distribution_graph = create_dist_graph( - gene_length_distribution, - "Length", - "Gene Length Distribution", - f"./{folder_name}/{os.path.basename(report_path)}.gene.length.png", - ) - - gene_score_distribution_graph = "" - if gene_score_distribution != []: - gene_score_distribution_graph = create_dist_graph( - gene_score_distribution, - "Score", - "Gene Score Distribution", - f"./{folder_name}/{os.path.basename(report_path)}.gene.score.png", - ) - - exon_length_distribution_graph = "" - if exon_length_distribution != []: - exon_length_distribution_graph = create_dist_graph( - exon_length_distribution, - "Length", - "Exon Length Distribution", - f"./{folder_name}/{os.path.basename(report_path)}.exon.length.png", - ) - - exon_number_distribution_graph = "" - if exon_number_distribution != []: - exon_number_distribution_graph = create_dist_graph( - exon_number_distribution, - "Number", - "Exon Number Distribution", - f"./{folder_name}/{os.path.basename(report_path)}.exon.number.png", - ) - - intron_length_distribution_graph = "" - if intron_length_distribution != []: - intron_length_distribution_graph = create_dist_graph( - intron_length_distribution, - "Length", - "Intron Length Distribution", - f"./{folder_name}/{os.path.basename(report_path)}.intron.length.png", - ) - - cds_length_distribution_graph = "" - if cds_length_distribution != []: - cds_length_distribution_graph = create_dist_graph( - intron_length_distribution, - "Length", - "CDS Length Distribution", - f"./{folder_name}/{os.path.basename(report_path)}.cds.length.png", - ) - - file_tag = re.findall( - r"([\w]+).gt.stat.yml", - os.path.basename(str(report_path)), - )[0] - - data["GENOMETOOLS_GT_STAT"].append( - { - "hap": file_tag, - "report_table": report_table_dict, - "report_table_html": tabulate( - pd.DataFrame( - report_table_dict.items(), columns=["Metric", "Value"] - ), - headers=["Stat", "Value"], - tablefmt="html", - numalign="left", - showindex=False, - ), - "gene_length_plot": gene_length_distribution_graph, - "gene_score_plot": gene_score_distribution_graph, - "exon_length_plot": exon_length_distribution_graph, - "exon_number_plot": exon_number_distribution_graph, - "intron_length_plot": intron_length_distribution_graph, - "cds_length_plot": cds_length_distribution_graph, - } - ) - - return { - "GENOMETOOLS_GT_STAT": sort_list_of_results(data["GENOMETOOLS_GT_STAT"], "hap") - } - - -def extract_report_data(report_path, num_groups): - yaml_data = {} - parent_key = "" - with open(report_path, "r") as stream: - for line in stream: - key, value = line.strip().split(":", 1) - - if value == "": - parent_key = key - yaml_data[parent_key] = {} - continue - - if parent_key == "": - yaml_data[key] = value.strip() - continue - - yaml_data[parent_key][key] = value.strip() - - report_table_dict = { - key: value for key, value in yaml_data.items() if not isinstance(value, dict) - } - gene_length_distribution = create_frequency_groups( - [ - (int(key), int(value.split("(")[0].strip())) - for key, value in yaml_data["gene length distribution"].items() - ], - num_groups, - ) - gene_score_distribution = create_frequency_groups( - [ - (int(key), int(value.split("(")[0].strip())) - for key, value in yaml_data["gene score distribution"].items() - ], - num_groups, - ) - exon_length_distribution = create_frequency_groups( - [ - (int(key), int(value.split("(")[0].strip())) - for key, value in yaml_data["exon length distribution"].items() - ], - num_groups, - ) - exon_number_distribution = create_frequency_groups( - [ - (int(key), int(value.split("(")[0].strip())) - for key, value in yaml_data["exon number distribution"].items() - ], - num_groups, - ) - intron_length_distribution = create_frequency_groups( - [ - (int(key), int(value.split("(")[0].strip())) - for key, value in yaml_data["intron length distribution"].items() - ], - num_groups, - ) - cds_length_distribution = create_frequency_groups( - [ - (int(key), int(value.split("(")[0].strip())) - for key, value in yaml_data["CDS length distribution"].items() - ], - num_groups, - ) - - return ( - report_table_dict, - gene_length_distribution, - gene_score_distribution, - exon_length_distribution, - exon_number_distribution, - intron_length_distribution, - cds_length_distribution, - ) - - -def create_frequency_groups(data, num_groups): - - if num_groups == -1: - sorted_data = sorted(data, key=lambda x: x[0]) - return [ - { - "start": x, - "stop": x, - "freq": freq, - } - for x, freq in sorted_data - ] - - assert ( - num_groups >= 1 - ), f"num_groups should be larger than or equal to 1. It is {num_groups}" - - if data == []: - return [] - - sorted_data = sorted(data, key=lambda x: x[0]) - - ordinal = [x for x, _ in sorted_data] - - ordinal_max = max(ordinal) - ordinal_range = ordinal_max - min(ordinal) - ordinal_step = ordinal_range // num_groups - - groups = [] - current_group = { - "start": sorted_data[0][0], - "stop": [x for x in ordinal if x <= (sorted_data[0][0] + ordinal_step)][-1], - "freq": 0, - } - - for num, freq in sorted_data: - if num <= current_group["stop"]: - current_group["freq"] += freq - continue - - groups.append(current_group.copy()) - - current_group["start"] = num - current_group["stop"] = [x for x in ordinal if x <= (num + ordinal_step)][-1] - current_group["freq"] = freq - - groups.append(current_group) - - return groups - - -def test_create_frequency_groups_multiple(): - data = [(15, 4), (5, 1), (70, 10)] - num_groups = 2 - - expect = [ - {"start": 5, "stop": 15, "freq": 5}, - {"start": 70, "stop": 70, "freq": 10}, - ] - - assert expect == create_frequency_groups(data, num_groups) - - -def test_create_frequency_groups_single(): - data = [(15, 4)] - num_groups = 2 - - expect = [{"start": 15, "stop": 15, "freq": 4}] - - assert expect == create_frequency_groups(data, num_groups) - - -def test_create_frequency_groups_repeat(): - data = [(15, 4), (15, 8)] - num_groups = 2 - - expect = [{"start": 15, "stop": 15, "freq": 12}] - - assert expect == create_frequency_groups(data, num_groups) - - -# test_create_frequency_groups_multiple() -# test_create_frequency_groups_single() -# test_create_frequency_groups_repeat() - - -def create_dist_graph(groups_dict, x_label, title, file_name): - - x_list = [i["stop"] for i in groups_dict] - y_list = [i["freq"] for i in groups_dict] - sum_y = float(sum(y_list)) - cum_sum_y = np.cumsum(y_list) - y_list = [float(y) / sum_y * 100.0 for y in cum_sum_y] - - _, ax = plt.subplots() - ax.plot(x_list, y_list) - - ax.set_xlabel(x_label) - ax.set_ylabel("Cumulative percentage (%)") - ax.set_title(title) - - plt.gca().spines["top"].set_visible(False) - plt.gca().spines["right"].set_visible(False) - - min_x, min_y = (min(x_list), min(y_list)) - x_anno_step = int(float(max(x_list)) * 0.1) - ax.annotate( - f"(<={min_x}, {round(min_y, 2)}%)", - xy=(min_x, min_y), - xytext=(min_x + x_anno_step, min_y + 10), - arrowprops=dict(color="red", arrowstyle="->, head_width=.15"), - ) - - near_50 = min([y for y in y_list if y >= 50.0]) - min_x, min_y = (x_list[y_list.index(near_50)], near_50) - ax.annotate( - f"(<={min_x}, {round(min_y, 2)}%)", - xy=(min_x, min_y), - xytext=(min_x + x_anno_step, min_y), - arrowprops=dict(color="red", arrowstyle="->, head_width=.15"), - ) - - near_90 = min([y for y in y_list if y >= 90.0]) - min_x, min_y = (x_list[y_list.index(near_90)], near_90) - ax.annotate( - f"(<={min_x}, {round(min_y, 2)}%)", - xy=(min_x, min_y), - xytext=(min_x + x_anno_step, min_y - 10), - arrowprops=dict(color="red", arrowstyle="->, head_width=.15"), - ) - - near_3_sigma = min([y for y in y_list if y >= 99.7]) - min_x, min_y = (x_list[y_list.index(near_3_sigma)], near_3_sigma) - ax.annotate( - f"(<={min_x}, {round(min_y, 2)}%)", - xy=(min_x, min_y), - xytext=(min_x + x_anno_step, min_y - 10), - arrowprops=dict(color="red", arrowstyle="->, head_width=.15"), - ) - - plt.savefig(file_name, dpi=600) - - with open(file_name, "rb") as f: - binary_fc = f.read() - - base64_utf8_str = base64.b64encode(binary_fc).decode("utf-8") - return f"data:image/png+xml;base64,{base64_utf8_str}" diff --git a/bin/report_modules/parsers/gff3_validate_parser.py b/bin/report_modules/parsers/gff3_validate_parser.py deleted file mode 100644 index e9a7d1e7..00000000 --- a/bin/report_modules/parsers/gff3_validate_parser.py +++ /dev/null @@ -1,35 +0,0 @@ -import os -from pathlib import Path -import re - -from report_modules.parsers.parsing_commons import sort_list_of_results - - -def parse_gff3_validate_folder(folder_name="gff3_validate_logs"): - dir = os.getcwdb().decode() - logs_folder_path = Path(f"{dir}/{folder_name}") - - if not os.path.exists(logs_folder_path): - return {} - - list_of_log_files = logs_folder_path.glob("*.log") - - data = {"GFF3_VALIDATE": []} - - for log_path in list_of_log_files: - with open(log_path, "r") as f: - log_lines = [f"

{l}

" for l in f.readlines()] - - file_tokens = re.findall( - r"([\w]+).error.log", - os.path.basename(str(log_path)), - )[0] - - data["GFF3_VALIDATE"].append( - { - "hap": file_tokens, - "validation_log": "".join(log_lines), - } - ) - - return {"GFF3_VALIDATE": sort_list_of_results(data["GFF3_VALIDATE"], "hap")} diff --git a/bin/report_modules/parsers/hic_parser.py b/bin/report_modules/parsers/hic_parser.py deleted file mode 100644 index 825852ec..00000000 --- a/bin/report_modules/parsers/hic_parser.py +++ /dev/null @@ -1,34 +0,0 @@ -import os -from pathlib import Path -import re - -from report_modules.parsers.parsing_commons import sort_list_of_results - - -def parse_hic_folder(folder_name="hic_outputs"): - dir = os.getcwdb().decode() - hic_folder_path = Path(f"{dir}/{folder_name}") - - if not os.path.exists(hic_folder_path): - return {} - - list_of_hic_files = hic_folder_path.glob("*.html") - - data = {"HIC": []} - - for hic_path in list_of_hic_files: - hic_file_name = os.path.basename(str(hic_path)) - - file_tokens = re.findall( - r"([\w]+).html", - hic_file_name, - )[0] - - data["HIC"].append( - { - "hap": file_tokens, - "hic_html_file_name": hic_file_name, - } - ) - - return {"HIC": sort_list_of_results(data["HIC"], "hap")} diff --git a/bin/report_modules/parsers/kraken2_parser.py b/bin/report_modules/parsers/kraken2_parser.py deleted file mode 100644 index f10fae13..00000000 --- a/bin/report_modules/parsers/kraken2_parser.py +++ /dev/null @@ -1,34 +0,0 @@ -import os -from pathlib import Path -import re - -from report_modules.parsers.parsing_commons import sort_list_of_results - - -def parse_kraken2_folder(folder_name="kraken2_outputs"): - dir = os.getcwdb().decode() - kraken2_folder_path = Path(f"{dir}/{folder_name}") - - if not os.path.exists(kraken2_folder_path): - return {} - - list_of_html_files = kraken2_folder_path.glob("*.html") - - data = {"KRAKEN2": []} - - for html_path in list_of_html_files: - html_file_name = os.path.basename(str(html_path)) - - file_tokens = re.findall( - r"([\w]+).kraken2.krona.html", - html_file_name, - )[0] - - data["KRAKEN2"].append( - { - "hap": file_tokens, - "krona_html_file_name": html_file_name, - } - ) - - return {"KRAKEN2": sort_list_of_results(data["KRAKEN2"], "hap")} diff --git a/bin/report_modules/parsers/lai_parser.py b/bin/report_modules/parsers/lai_parser.py deleted file mode 100644 index 7318e92a..00000000 --- a/bin/report_modules/parsers/lai_parser.py +++ /dev/null @@ -1,95 +0,0 @@ -import os -from pathlib import Path -import re - -from report_modules.parsers.parsing_commons import sort_list_of_results - - -class LAIParser: - def __init__(self, log_file_data, out_file_data): - self.log_file_data = log_file_data - self.out_file_data = out_file_data - self.stats_dict = {} - - def parse_report(self): - self.stats_dict["version"] = self.get_lai_version() - lai_errors = self.get_lai_errors() - - if lai_errors != None: - self.stats_dict["result"] = lai_errors - return self.stats_dict - - lai_stats = self.get_lai_stats() - - self.stats_dict["result"] = lai_stats - return self.stats_dict - - def get_lai_version(self): - p = re.compile("### LTR Assembly Index \(LAI\) (.*) ###") - result = p.search(self.log_file_data).group(1).strip() - return result - - def get_lai_errors(self): - p = re.compile("【Error】(.*)") - match_results = p.findall(self.log_file_data) - if len(match_results) < 1: - return None - - return ". ".join([m.strip() for m in match_results]) - - def get_lai_stats(self): - p = re.compile(r"whole_genome(.*)") - match_results = p.findall(self.out_file_data) - if len(match_results) != 1: - return "Error parsing the LAI.out file" - - raw_stats = match_results[0].strip().split("\t") - - if len(raw_stats) != 6: - return "Error parsing the LAI.out file" - - stats_str = f"Intact: {raw_stats[2]}, Total: {raw_stats[3]}, Raw LAI: {raw_stats[4]}, LAI: {raw_stats[5]}" - - return stats_str - - -def parse_lai_folder(folder_name="lai_outputs"): - dir = os.getcwdb().decode() - lai_folder_path = Path(f"{dir}/{folder_name}") - - if not os.path.exists(lai_folder_path): - return {} - - list_of_log_files = lai_folder_path.glob("*.LAI.log") - - data = {"LAI": []} - - for file in list_of_log_files: - log_file_data = "" - with open(file, "r") as file: - lines = file.readlines() - for line in lines: - log_file_data += line - - file_tokens = re.findall( - r"([\w]+).LAI.log", - os.path.basename(str(file)), - ) - - hap_name = file_tokens[0] - out_file_path = Path(f"{dir}/{folder_name}/{hap_name}.LAI.out") - out_file_data = "" - if os.path.exists(out_file_path): - with open(out_file_path, "r") as out_file: - lines = out_file.readlines() - for line in lines: - out_file_data += line - - parser = LAIParser(log_file_data, out_file_data) - stats = { - "hap": hap_name, - **parser.parse_report(), - } - data["LAI"].append(stats) - - return {"LAI": sort_list_of_results(data["LAI"], "hap")} diff --git a/bin/report_modules/parsers/ncbi_fcs_adaptor_parser.py b/bin/report_modules/parsers/ncbi_fcs_adaptor_parser.py deleted file mode 100644 index f896577f..00000000 --- a/bin/report_modules/parsers/ncbi_fcs_adaptor_parser.py +++ /dev/null @@ -1,44 +0,0 @@ -import os -from pathlib import Path -import pandas as pd -from tabulate import tabulate -import re - -from report_modules.parsers.parsing_commons import sort_list_of_results - - -def parse_ncbi_fcs_adaptor_folder(folder_name="ncbi_fcs_adaptor_reports"): - dir = os.getcwdb().decode() - reports_folder_path = Path(f"{dir}/{folder_name}") - - if not os.path.exists(reports_folder_path): - return {} - - list_of_report_files = reports_folder_path.glob("*.tsv") - - data = {"NCBI_FCS_ADAPTOR": []} - - for report_path in list_of_report_files: - report_table = pd.read_csv(report_path, sep="\t") - - file_tokens = re.findall( - r"([\w]+)_fcs_adaptor_report.tsv", - os.path.basename(str(report_path)), - )[0] - - data["NCBI_FCS_ADAPTOR"].append( - { - "hap": file_tokens, - "did_detect_contamination": report_table.shape[0] > 0, - "report_table": report_table.to_dict("records"), - "report_table_html": tabulate( - report_table, - headers=["Accession No.", "Length", "Action", "Range", "Name"], - tablefmt="html", - numalign="left", - showindex=False, - ), - } - ) - - return {"NCBI_FCS_ADAPTOR": sort_list_of_results(data["NCBI_FCS_ADAPTOR"], "hap")} diff --git a/bin/report_modules/parsers/ncbi_fcs_gx_parser.py b/bin/report_modules/parsers/ncbi_fcs_gx_parser.py deleted file mode 100644 index 8ee14e21..00000000 --- a/bin/report_modules/parsers/ncbi_fcs_gx_parser.py +++ /dev/null @@ -1,56 +0,0 @@ -import os -from pathlib import Path -import pandas as pd -from tabulate import tabulate -import re -import json - -from report_modules.parsers.parsing_commons import sort_list_of_results - - -def parse_ncbi_fcs_gx_folder(folder_name="fcs_gx_reports"): - dir = os.getcwdb().decode() - reports_folder_path = Path(f"{dir}/{folder_name}") - - if not os.path.exists(reports_folder_path): - return {} - - list_of_report_files = reports_folder_path.glob("*.txt") - - data = {"NCBI_FCS_GX": []} - - for report_path in list_of_report_files: - with open(report_path, "r") as f: - meta_data = json.loads(f.readline()[2:-1]) - - asserted_div = meta_data[1]["run-info"]["asserted-div"] - inferred_primary_divs = meta_data[1]["run-info"]["inferred-primary-divs"] - - report_table = pd.read_csv(report_path, sep="\t", skiprows=1) - - file_tokens = re.findall( - r"([\w]+).fcs_gx_report.txt", - os.path.basename(str(report_path)), - )[0] - - data["NCBI_FCS_GX"].append( - { - "hap": file_tokens, - "did_detect_contamination": report_table.shape[0] > 0, - "report_table": report_table.to_dict("records"), - "report_table_html": tabulate( - report_table.iloc[:, [0, 1, 2, 3, 4, 7]], - headers=["Seq ID", "Start", "End", "Length", "Action", "Tax name"], - tablefmt="html", - numalign="left", - showindex=False, - ), - "report_meta_data": meta_data, - "is_wrong_div": False - if asserted_div in inferred_primary_divs - else True, - "krona_html_file_name": f"{file_tokens}.fcs.gx.krona.html", - } - ) - - return {"NCBI_FCS_GX": sort_list_of_results(data["NCBI_FCS_GX"], "hap")} diff --git a/bin/report_modules/parsers/params_parser.py b/bin/report_modules/parsers/params_parser.py deleted file mode 100644 index bf07dc5a..00000000 --- a/bin/report_modules/parsers/params_parser.py +++ /dev/null @@ -1,43 +0,0 @@ -import json - -from pygments import highlight -from pygments.lexers import JsonLexer -from pygments.formatters import HtmlFormatter - - -def highlight_json(json_string): - lexer = JsonLexer() - formatter = HtmlFormatter() - - return highlight(json_string, lexer, formatter) - - -def format_params_dict(json_dict): - formatted_dict = {} - for key, value in json_dict.items(): - if key in ["max_cpus", "max_memory", "max_time"]: - continue - - if not isinstance(value, dict): - formatted_dict[key] = value - continue - - if "skip" in value.keys(): - if value["skip"] == 1: - formatted_dict[key] = "Skipped" - continue - - formatted_dict[key] = value - formatted_dict[key].pop("skip", None) - - return formatted_dict - - -def parse_params_json(): - with open("params_json.json", "r") as f: - params_dict = json.load(f) - formatted_dict_json = highlight_json( - json.dumps(format_params_dict(params_dict), indent=4) - ) - - return params_dict, formatted_dict_json diff --git a/bin/report_modules/parsers/parsing_commons.py b/bin/report_modules/parsers/parsing_commons.py deleted file mode 100644 index ff46c481..00000000 --- a/bin/report_modules/parsers/parsing_commons.py +++ /dev/null @@ -1,10 +0,0 @@ -import re - - -def natural_key(string): - """Return a list of keys that sort naturally.""" - return [int(s) if s.isdigit() else s for s in re.split(r"(\d+)", string)] - - -def sort_list_of_results(results_list, on_key): - return sorted(results_list, key=lambda x: natural_key(x[on_key])) diff --git a/bin/report_modules/parsers/tidk_parser.py b/bin/report_modules/parsers/tidk_parser.py deleted file mode 100644 index 556d93a4..00000000 --- a/bin/report_modules/parsers/tidk_parser.py +++ /dev/null @@ -1,68 +0,0 @@ -import os -from pathlib import Path -import base64 -import re - -from report_modules.parsers.parsing_commons import sort_list_of_results - - -def parse_tidk_folder(folder_name="tidk_outputs"): - dir = os.getcwdb().decode() - tidk_folder_path = Path(f"{dir}/{folder_name}") - - if not os.path.exists(tidk_folder_path): - return {} - - list_of_plot_files = tidk_folder_path.glob("*.svg") - - data = {"TIDK": []} - - # get the a_prior_sequence file - a_priori_sequence_file_name = "a_priori.sequence" - with open(f"{dir}/{folder_name}/{a_priori_sequence_file_name}", "r") as file: - lines = file.readlines() - a_priori_sequence = lines[0].strip() - - for plot_path in list_of_plot_files: - binary_fc = open(plot_path, "rb").read() - base64_utf8_str = base64.b64encode(binary_fc).decode("utf-8") - ext = str(plot_path).split(".")[-1] - plot_url = f"data:image/{ext}+xml;base64,{base64_utf8_str}" - - file_tokens = re.findall( - r"([\w]+).([\w]+).svg", - os.path.basename(str(plot_path)), - )[0] - - sample_tag = file_tokens[0].strip() - plot_type = file_tokens[1].strip() - if "aposteriori" in plot_type: - sequence_file_name = f"{sample_tag}.top.sequence.txt" - - with open(f"{dir}/{folder_name}/{sequence_file_name}", "r") as file: - lines = file.readlines() - sequence = "" if len(lines) < 1 else lines[0].strip() - - display_name = f"{sample_tag}: a posteriori sequence" - - else: - display_name = f"{sample_tag}: a priori sequence" - sequence = "" - - data["TIDK"].append( - { - "hap": f"{sample_tag}_{plot_type}", - "hap_display": display_name, - "sequence": sequence, - "is_a_priori": "a priori" in display_name, - "a_priori_sequence": a_priori_sequence, - "has_sequence": sequence != "", - "tidk_plot": plot_url, - "tidk_plot_empty": False, - } - ) - - if len(data["TIDK"]) < 1: - return {} - - return {"TIDK": sort_list_of_results(data["TIDK"], "hap")} diff --git a/bin/report_modules/parsers/tools_parser.py b/bin/report_modules/parsers/tools_parser.py deleted file mode 100644 index 54746094..00000000 --- a/bin/report_modules/parsers/tools_parser.py +++ /dev/null @@ -1,33 +0,0 @@ -import yaml -import json - -from pygments import highlight -from pygments.lexers import JsonLexer -from pygments.formatters import HtmlFormatter - - -def parse_tools_yaml(): - with open("software_versions.yml", "r") as f: - tools_dict = yaml.safe_load(f) - formatted_tools_json = highlight_json( - json.dumps(format_tools_dict(tools_dict), indent=4) - ) - - return tools_dict, formatted_tools_json - - -def highlight_json(json_string): - lexer = JsonLexer() - formatter = HtmlFormatter() - - return highlight(json_string, lexer, formatter) - - -def format_tools_dict(input_dict): - output_list = [] - for _, top_level_value in input_dict.items(): - for key, value in top_level_value.items(): - if (key, value) not in output_list: - output_list.append((key, value)) - - return dict(sorted(output_list, key=lambda x: x[0])) diff --git a/bin/report_modules/report_printer.py b/bin/report_modules/report_printer.py deleted file mode 100644 index 24e58f7a..00000000 --- a/bin/report_modules/report_printer.py +++ /dev/null @@ -1,15 +0,0 @@ -from jinja2 import Environment, FileSystemLoader -from pathlib import Path - - -class ReportPrinter: - def __init__(self): - projectDir = "/".join(__file__.split("/")[0:-1]) - path = Path(f"{projectDir}/templates") - - self.file_loader = FileSystemLoader(path) - self.env = Environment(loader=self.file_loader) - - def print_template(self, stats): - template = self.env.get_template("base.html") - print(template.render(all_stats_dicts=stats)) diff --git a/bin/report_modules/templates/assemblathon_stats/assemblathon_stats.html b/bin/report_modules/templates/assemblathon_stats/assemblathon_stats.html deleted file mode 100644 index d8e5b82d..00000000 --- a/bin/report_modules/templates/assemblathon_stats/assemblathon_stats.html +++ /dev/null @@ -1,21 +0,0 @@ - diff --git a/bin/report_modules/templates/assemblathon_stats/dropdown.html b/bin/report_modules/templates/assemblathon_stats/dropdown.html deleted file mode 100644 index 8be7bfa6..00000000 --- a/bin/report_modules/templates/assemblathon_stats/dropdown.html +++ /dev/null @@ -1,10 +0,0 @@ -
-
- -
-
diff --git a/bin/report_modules/templates/assemblathon_stats/report_contents.html b/bin/report_modules/templates/assemblathon_stats/report_contents.html deleted file mode 100644 index 34bf5f7e..00000000 --- a/bin/report_modules/templates/assemblathon_stats/report_contents.html +++ /dev/null @@ -1,17 +0,0 @@ -{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["ASSEMBLATHON_STATS"]|length) %} {% set -active_text = 'display: block' if vars.is_first else 'display: none' %} -
-
-
-
{{ all_stats_dicts['ASSEMBLATHON_STATS'][item]['hap'] }}
-
-
-
-
{{ all_stats_dicts['ASSEMBLATHON_STATS'][item]['report_table_html'] }}
-
-
-{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/bin/report_modules/templates/base.html b/bin/report_modules/templates/base.html deleted file mode 100644 index e0b8eef0..00000000 --- a/bin/report_modules/templates/base.html +++ /dev/null @@ -1,68 +0,0 @@ - - {% include 'header.html' %} - - -
-
AssemblyQC {{ all_stats_dicts['VERSIONS']['Workflow']['AssemblyQC'] }}
-
-
- - - {% if 'GFF3_VALIDATE' in all_stats_dicts %} - - {% endif %} {% if 'FASTA_VALIDATE' in all_stats_dicts %} - - {% endif %} {% if 'NCBI_FCS_ADAPTOR' in all_stats_dicts %} - - {% endif %} {% if 'NCBI_FCS_GX' in all_stats_dicts %}{% endif %} {% if 'ASSEMBLATHON_STATS' in all_stats_dicts %}{% endif %} {% if 'GENOMETOOLS_GT_STAT' in all_stats_dicts %}{% endif %} {% if 'BUSCO' in all_stats_dicts %}{% endif %} {% if 'TIDK' in all_stats_dicts %}{% endif %} {% if 'LAI' in all_stats_dicts %}{% endif %} {% if 'KRAKEN2' in all_stats_dicts %}{% endif %} {% if 'HIC' in all_stats_dicts %}{% endif %} {% if 'CIRCOS' in all_stats_dicts %}{% endif %} -
- {% include 'params/params.html' %} {% include 'tools/tools.html' %} {% if 'GFF3_VALIDATE' in all_stats_dicts - %}{% include 'gff3_validate/gff3_validate.html' %}{% endif %} {% if 'FASTA_VALIDATE' in all_stats_dicts %}{% - include 'fasta_validate/fasta_validate.html' %}{% endif %} {% if 'NCBI_FCS_ADAPTOR' in all_stats_dicts %}{% - include 'ncbi_fcs_adaptor/ncbi_fcs_adaptor.html' %}{% endif %} {% if 'NCBI_FCS_GX' in all_stats_dicts %}{% - include 'ncbi_fcs_gx/ncbi_fcs_gx.html' %}{% endif %} {% if 'ASSEMBLATHON_STATS' in all_stats_dicts %}{% include - 'assemblathon_stats/assemblathon_stats.html' %}{% endif %} {% if 'GENOMETOOLS_GT_STAT' in all_stats_dicts %}{% - include 'genometools_gt_stat/genometools_gt_stat.html' %}{% endif %} {% if 'BUSCO' in all_stats_dicts %}{% - include 'busco/busco.html' %}{% endif %} {% if 'TIDK' in all_stats_dicts %}{% include 'tidk/tidk.html' %}{% - endif %} {% if 'LAI' in all_stats_dicts %}{% include 'lai/lai.html' %}{% endif %} {% if 'KRAKEN2' in - all_stats_dicts %}{% include 'kraken2/kraken2.html' %}{% endif %} {% if 'HIC' in all_stats_dicts %}{% include - 'hic/hic.html' %}{% endif %} {% if 'CIRCOS' in all_stats_dicts %}{% include 'circos/circos.html' %}{% endif %} - - {% include 'js.html' %} - diff --git a/bin/report_modules/templates/busco/busco.html b/bin/report_modules/templates/busco/busco.html deleted file mode 100644 index 06d560e5..00000000 --- a/bin/report_modules/templates/busco/busco.html +++ /dev/null @@ -1,18 +0,0 @@ - diff --git a/bin/report_modules/templates/busco/dropdown.html b/bin/report_modules/templates/busco/dropdown.html deleted file mode 100644 index 23981b8c..00000000 --- a/bin/report_modules/templates/busco/dropdown.html +++ /dev/null @@ -1,13 +0,0 @@ -
-
- -
-
diff --git a/bin/report_modules/templates/busco/report_contents.html b/bin/report_modules/templates/busco/report_contents.html deleted file mode 100644 index 1c552652..00000000 --- a/bin/report_modules/templates/busco/report_contents.html +++ /dev/null @@ -1,77 +0,0 @@ -{% for item in range(all_stats_dicts["BUSCO"]|length) %} - -{% endfor %} diff --git a/bin/report_modules/templates/busco/summary_contents.html b/bin/report_modules/templates/busco/summary_contents.html deleted file mode 100644 index d289b4aa..00000000 --- a/bin/report_modules/templates/busco/summary_contents.html +++ /dev/null @@ -1,37 +0,0 @@ -
-
-
-
Summary
-
- -
-
- - - - - - - - - - {% for item in range(all_stats_dicts["BUSCO"]|length) %} - - - - - - {% endfor %} - -
AssemblyLineagePercentages
- {{ all_stats_dicts['BUSCO'][item]['hap'] }} - - {{ all_stats_dicts['BUSCO'][item]['lineage'] }} - {{ all_stats_dicts['BUSCO'][item]['search_percentages'] }}
-
-
-
-
- -
-
diff --git a/bin/report_modules/templates/circos/circos.html b/bin/report_modules/templates/circos/circos.html deleted file mode 100644 index 2099a251..00000000 --- a/bin/report_modules/templates/circos/circos.html +++ /dev/null @@ -1,32 +0,0 @@ - diff --git a/bin/report_modules/templates/circos/dropdown.html b/bin/report_modules/templates/circos/dropdown.html deleted file mode 100644 index 0cdafbb7..00000000 --- a/bin/report_modules/templates/circos/dropdown.html +++ /dev/null @@ -1,10 +0,0 @@ -
-
- -
-
diff --git a/bin/report_modules/templates/circos/report_contents.html b/bin/report_modules/templates/circos/report_contents.html deleted file mode 100644 index e3355169..00000000 --- a/bin/report_modules/templates/circos/report_contents.html +++ /dev/null @@ -1,26 +0,0 @@ -{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["CIRCOS"]|length) %} {% set active_text = -'display: block' if vars.is_first else 'display: none' %} -
-
-
-
{{ all_stats_dicts['CIRCOS'][item]['tag.on.tag'] }}
-
- {% if all_stats_dicts['CIRCOS'][item]['is_plot_empty'] %} -
-

- Either no alignments were detected between the target and reference assemblies, or all the alignment - bundles were filtered out 😔 -

-
- {% else %} -
- -
- {% endif %} -
-
-{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/bin/report_modules/templates/fasta_validate/dropdown.html b/bin/report_modules/templates/fasta_validate/dropdown.html deleted file mode 100644 index 56fb72ef..00000000 --- a/bin/report_modules/templates/fasta_validate/dropdown.html +++ /dev/null @@ -1,10 +0,0 @@ -
-
- -
-
diff --git a/bin/report_modules/templates/fasta_validate/fasta_validate.html b/bin/report_modules/templates/fasta_validate/fasta_validate.html deleted file mode 100644 index e0643542..00000000 --- a/bin/report_modules/templates/fasta_validate/fasta_validate.html +++ /dev/null @@ -1,14 +0,0 @@ - diff --git a/bin/report_modules/templates/fasta_validate/report_contents.html b/bin/report_modules/templates/fasta_validate/report_contents.html deleted file mode 100644 index 7abaee35..00000000 --- a/bin/report_modules/templates/fasta_validate/report_contents.html +++ /dev/null @@ -1,18 +0,0 @@ -{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["FASTA_VALIDATE"]|length) %} {% set active_text -= 'display: block' if vars.is_first else 'display: none' %} -
-
-
-
{{ all_stats_dicts['FASTA_VALIDATE'][item]['hap'] }}
-
-
-

Validation failed!

-
-
{{ all_stats_dicts['FASTA_VALIDATE'][item]['validation_log'] }}
-
-
-{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/bin/report_modules/templates/genometools_gt_stat/dropdown.html b/bin/report_modules/templates/genometools_gt_stat/dropdown.html deleted file mode 100644 index cd948bdf..00000000 --- a/bin/report_modules/templates/genometools_gt_stat/dropdown.html +++ /dev/null @@ -1,10 +0,0 @@ -
-
- -
-
diff --git a/bin/report_modules/templates/genometools_gt_stat/genometools_gt_stat.html b/bin/report_modules/templates/genometools_gt_stat/genometools_gt_stat.html deleted file mode 100644 index c5a5e7a1..00000000 --- a/bin/report_modules/templates/genometools_gt_stat/genometools_gt_stat.html +++ /dev/null @@ -1,15 +0,0 @@ - diff --git a/bin/report_modules/templates/genometools_gt_stat/report_contents.html b/bin/report_modules/templates/genometools_gt_stat/report_contents.html deleted file mode 100644 index d2975ede..00000000 --- a/bin/report_modules/templates/genometools_gt_stat/report_contents.html +++ /dev/null @@ -1,35 +0,0 @@ -{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["GENOMETOOLS_GT_STAT"]|length) %} {% set -active_text = 'display: block' if vars.is_first else 'display: none' %} -
-
-
-
{{ all_stats_dicts['GENOMETOOLS_GT_STAT'][item]['hap'] }}
-
-
-
-
{{ all_stats_dicts['GENOMETOOLS_GT_STAT'][item]['report_table_html'] }}
-
-
- -
-
- -
-
- -
-
- -
-
- -
-
- -
-
-{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/bin/report_modules/templates/gff3_validate/dropdown.html b/bin/report_modules/templates/gff3_validate/dropdown.html deleted file mode 100644 index 0bece7a0..00000000 --- a/bin/report_modules/templates/gff3_validate/dropdown.html +++ /dev/null @@ -1,10 +0,0 @@ -
-
- -
-
diff --git a/bin/report_modules/templates/gff3_validate/gff3_validate.html b/bin/report_modules/templates/gff3_validate/gff3_validate.html deleted file mode 100644 index 01fa2754..00000000 --- a/bin/report_modules/templates/gff3_validate/gff3_validate.html +++ /dev/null @@ -1,22 +0,0 @@ - diff --git a/bin/report_modules/templates/gff3_validate/report_contents.html b/bin/report_modules/templates/gff3_validate/report_contents.html deleted file mode 100644 index d61358a2..00000000 --- a/bin/report_modules/templates/gff3_validate/report_contents.html +++ /dev/null @@ -1,18 +0,0 @@ -{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["GFF3_VALIDATE"]|length) %} {% set active_text -= 'display: block' if vars.is_first else 'display: none' %} -
-
-
-
{{ all_stats_dicts['GFF3_VALIDATE'][item]['hap'] }}
-
-
-

Validation failed!

-
-
{{ all_stats_dicts['GFF3_VALIDATE'][item]['validation_log'] }}
-
-
-{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/bin/report_modules/templates/header.html b/bin/report_modules/templates/header.html deleted file mode 100644 index 989b37f0..00000000 --- a/bin/report_modules/templates/header.html +++ /dev/null @@ -1,637 +0,0 @@ - - AssemblyQC - - - - - - - - diff --git a/bin/report_modules/templates/hic/dropdown.html b/bin/report_modules/templates/hic/dropdown.html deleted file mode 100644 index 4cd50f43..00000000 --- a/bin/report_modules/templates/hic/dropdown.html +++ /dev/null @@ -1,10 +0,0 @@ -
-
- -
-
diff --git a/bin/report_modules/templates/hic/hic.html b/bin/report_modules/templates/hic/hic.html deleted file mode 100644 index 868dc089..00000000 --- a/bin/report_modules/templates/hic/hic.html +++ /dev/null @@ -1,18 +0,0 @@ - diff --git a/bin/report_modules/templates/hic/hic_html_template.html b/bin/report_modules/templates/hic/hic_html_template.html deleted file mode 100644 index 6d3f4088..00000000 --- a/bin/report_modules/templates/hic/hic_html_template.html +++ /dev/null @@ -1,92 +0,0 @@ - - - - - HiC Contact Map - - - - - - - - -
-
-
- - - diff --git a/bin/report_modules/templates/hic/report_contents.html b/bin/report_modules/templates/hic/report_contents.html deleted file mode 100644 index 312a0fdd..00000000 --- a/bin/report_modules/templates/hic/report_contents.html +++ /dev/null @@ -1,17 +0,0 @@ -{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["HIC"]|length) %} {% set active_text = -'display: block' if vars.is_first else 'display: none' %} -
-
-
-
{{ all_stats_dicts['HIC'][item]['hap'] }}
-
-
- -
-
-
-{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/bin/report_modules/templates/js.html b/bin/report_modules/templates/js.html deleted file mode 100644 index fd0f8d21..00000000 --- a/bin/report_modules/templates/js.html +++ /dev/null @@ -1,58 +0,0 @@ - diff --git a/bin/report_modules/templates/kraken2/dropdown.html b/bin/report_modules/templates/kraken2/dropdown.html deleted file mode 100644 index cced9430..00000000 --- a/bin/report_modules/templates/kraken2/dropdown.html +++ /dev/null @@ -1,11 +0,0 @@ -
-
- -
-
diff --git a/bin/report_modules/templates/kraken2/kraken2.html b/bin/report_modules/templates/kraken2/kraken2.html deleted file mode 100644 index 1b87e9e2..00000000 --- a/bin/report_modules/templates/kraken2/kraken2.html +++ /dev/null @@ -1,15 +0,0 @@ - diff --git a/bin/report_modules/templates/kraken2/report_contents.html b/bin/report_modules/templates/kraken2/report_contents.html deleted file mode 100644 index 65f09183..00000000 --- a/bin/report_modules/templates/kraken2/report_contents.html +++ /dev/null @@ -1,21 +0,0 @@ -{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["KRAKEN2"]|length) %} {% set active_text = -'display: block' if vars.is_first else 'display: none' %} -
-
-
-
{{ all_stats_dicts['KRAKEN2'][item]['hap'] }}
-
-
- -
-
-
-{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/bin/report_modules/templates/lai/dropdown.html b/bin/report_modules/templates/lai/dropdown.html deleted file mode 100644 index ca7e0c1b..00000000 --- a/bin/report_modules/templates/lai/dropdown.html +++ /dev/null @@ -1,7 +0,0 @@ -
-
- -
-
diff --git a/bin/report_modules/templates/lai/lai.html b/bin/report_modules/templates/lai/lai.html deleted file mode 100644 index 4b0775ae..00000000 --- a/bin/report_modules/templates/lai/lai.html +++ /dev/null @@ -1,26 +0,0 @@ - diff --git a/bin/report_modules/templates/lai/summary_contents.html b/bin/report_modules/templates/lai/summary_contents.html deleted file mode 100644 index 7844edb9..00000000 --- a/bin/report_modules/templates/lai/summary_contents.html +++ /dev/null @@ -1,30 +0,0 @@ -
-
-
-
Summary
-
- -
-
- - - - - - - - - {% for item in range(all_stats_dicts["LAI"]|length) %} - - - - - {% endfor %} - -
AssemblyResults
{{ all_stats_dicts['LAI'][item]['hap'] }} - {{ all_stats_dicts['LAI'][item]['result'] }} -
-
-
-
-
diff --git a/bin/report_modules/templates/ncbi_fcs_adaptor/dropdown.html b/bin/report_modules/templates/ncbi_fcs_adaptor/dropdown.html deleted file mode 100644 index 9f21bc26..00000000 --- a/bin/report_modules/templates/ncbi_fcs_adaptor/dropdown.html +++ /dev/null @@ -1,10 +0,0 @@ -
-
- -
-
diff --git a/bin/report_modules/templates/ncbi_fcs_adaptor/ncbi_fcs_adaptor.html b/bin/report_modules/templates/ncbi_fcs_adaptor/ncbi_fcs_adaptor.html deleted file mode 100644 index e62678c9..00000000 --- a/bin/report_modules/templates/ncbi_fcs_adaptor/ncbi_fcs_adaptor.html +++ /dev/null @@ -1,11 +0,0 @@ - diff --git a/bin/report_modules/templates/ncbi_fcs_adaptor/report_contents.html b/bin/report_modules/templates/ncbi_fcs_adaptor/report_contents.html deleted file mode 100644 index ae1cd77b..00000000 --- a/bin/report_modules/templates/ncbi_fcs_adaptor/report_contents.html +++ /dev/null @@ -1,24 +0,0 @@ -{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["NCBI_FCS_ADAPTOR"]|length) %} {% set -active_text = 'display: block' if vars.is_first else 'display: none' %} -
-
-
-
{{ all_stats_dicts['NCBI_FCS_ADAPTOR'][item]['hap'] }}
-
-
- {%if all_stats_dicts['NCBI_FCS_ADAPTOR'][item]['did_detect_contamination'] %} -
-

Contamination detected. Subsequent pipeline modules are skipped.

-
-
-
{{ all_stats_dicts['NCBI_FCS_ADAPTOR'][item]['report_table_html'] }}
-
- {% else %} -

No contamination detected.

- {% endif %} -
-{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/bin/report_modules/templates/ncbi_fcs_gx/dropdown.html b/bin/report_modules/templates/ncbi_fcs_gx/dropdown.html deleted file mode 100644 index 594f3e6f..00000000 --- a/bin/report_modules/templates/ncbi_fcs_gx/dropdown.html +++ /dev/null @@ -1,10 +0,0 @@ -
-
- -
-
diff --git a/bin/report_modules/templates/ncbi_fcs_gx/ncbi_fcs_gx.html b/bin/report_modules/templates/ncbi_fcs_gx/ncbi_fcs_gx.html deleted file mode 100644 index e2c2a338..00000000 --- a/bin/report_modules/templates/ncbi_fcs_gx/ncbi_fcs_gx.html +++ /dev/null @@ -1,23 +0,0 @@ - diff --git a/bin/report_modules/templates/ncbi_fcs_gx/report_contents.html b/bin/report_modules/templates/ncbi_fcs_gx/report_contents.html deleted file mode 100644 index c99c5622..00000000 --- a/bin/report_modules/templates/ncbi_fcs_gx/report_contents.html +++ /dev/null @@ -1,49 +0,0 @@ -{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["NCBI_FCS_GX"]|length) %} {% set active_text = -'display: block' if vars.is_first else 'display: none' %} -
-
-
-
{{ all_stats_dicts['NCBI_FCS_GX'][item]['hap'] }}
-
- {%if all_stats_dicts['NCBI_FCS_GX'][item]['did_detect_contamination'] %} -
-

Contamination detected. Subsequent pipeline modules are skipped.

-
-
-
{{ all_stats_dicts['NCBI_FCS_GX'][item]['report_table_html'] }}
-
- {% else %} -
-

No contamination detected.

-
- {% endif %} {%if all_stats_dicts['NCBI_FCS_GX'][item]['is_wrong_div'] %} -
-

- Warning: Asserted div ({{ - all_stats_dicts['NCBI_FCS_GX'][item]['report_meta_data'][1]["run-info"]["asserted-div"] }}) is not - present in the inferred div(s) ({{ - all_stats_dicts['NCBI_FCS_GX'][item]['report_meta_data'][1]["run-info"]["inferred-primary-divs"] }}). - Either the tax ID is incorrect or the sample is predominantly contamination. -

-
- {% endif %} -
-
Taxonomy
-
-
-

Bogus, Repeat, Low-coverage and Inconclusive results are labelled as No hits.

-
-
- -
-
-
-{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/bin/report_modules/templates/params/contents.html b/bin/report_modules/templates/params/contents.html deleted file mode 100644 index 7c10ad16..00000000 --- a/bin/report_modules/templates/params/contents.html +++ /dev/null @@ -1,8 +0,0 @@ -
-
-
-
Pipeline Parameters
-
- {{ all_stats_dicts['PARAMS_TABLE'] }} -
-
diff --git a/bin/report_modules/templates/params/params.html b/bin/report_modules/templates/params/params.html deleted file mode 100644 index 276d1e15..00000000 --- a/bin/report_modules/templates/params/params.html +++ /dev/null @@ -1,19 +0,0 @@ - diff --git a/bin/report_modules/templates/tidk/dropdown.html b/bin/report_modules/templates/tidk/dropdown.html deleted file mode 100644 index 602bd367..00000000 --- a/bin/report_modules/templates/tidk/dropdown.html +++ /dev/null @@ -1,11 +0,0 @@ -
-
- -
-
diff --git a/bin/report_modules/templates/tidk/report_contents.html b/bin/report_modules/templates/tidk/report_contents.html deleted file mode 100644 index 9c81c00d..00000000 --- a/bin/report_modules/templates/tidk/report_contents.html +++ /dev/null @@ -1,33 +0,0 @@ -{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["TIDK"]|length) %} {% set active_text = -'display: block' if vars.is_first else 'display: none' %} -
-
-
-
{{ all_stats_dicts['TIDK'][item]['hap_display'] }}
-
- {% if all_stats_dicts['TIDK'][item]['has_sequence'] %} -
-

- Searched sequence: - {{ all_stats_dicts['TIDK'][item]['sequence'] }} -

-
- {% endif %} {% if all_stats_dicts['TIDK'][item]['is_a_priori'] %} -
-

- Searched sequence: - {{ all_stats_dicts['TIDK'][item]['a_priori_sequence'] }} -

-
- {% endif %} {% if all_stats_dicts['TIDK'][item]['tidk_plot_empty'] %} -
-

TIDK explore could not find a repeat sequence 😔

-
- {% else %} -
- -
- {% endif %} -
-
-{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/bin/report_modules/templates/tidk/tidk.html b/bin/report_modules/templates/tidk/tidk.html deleted file mode 100644 index 0503dae9..00000000 --- a/bin/report_modules/templates/tidk/tidk.html +++ /dev/null @@ -1,15 +0,0 @@ - diff --git a/bin/report_modules/templates/tools/contents.html b/bin/report_modules/templates/tools/contents.html deleted file mode 100644 index 721721b6..00000000 --- a/bin/report_modules/templates/tools/contents.html +++ /dev/null @@ -1,9 +0,0 @@ -
-
-
-
Pipeline Tools
-

Following is a non-exhaustive list of tools used to generate this report.

-
- {{ all_stats_dicts['TOOLS_TABLE'] }} -
-
diff --git a/bin/report_modules/templates/tools/tools.html b/bin/report_modules/templates/tools/tools.html deleted file mode 100644 index 4d78232c..00000000 --- a/bin/report_modules/templates/tools/tools.html +++ /dev/null @@ -1 +0,0 @@ - diff --git a/bin/validate_seq_lists_1d50376.sh b/bin/validate_seq_lists_1d50376.sh deleted file mode 100755 index 959c87d9..00000000 --- a/bin/validate_seq_lists_1d50376.sh +++ /dev/null @@ -1,39 +0,0 @@ -#!/usr/bin/env bash - -seqFileA=$1 -seqFileB=$2 - -linesFileA=() -linesFileB=() - -while IFS= read -r line; do - linesFileA+=("$line") - columns=($line) - if [[ ${#columns[@]} -ne 2 ]]; then - echo "Error: Sequence file $(basename "$seqFileA") does not have exactly two columns." >&2 - exit 1 - fi -done < "$seqFileA" - -while IFS= read -r line; do - linesFileB+=("$line") - columns=($line) - if [[ ${#columns[@]} -ne 2 ]]; then - echo "Error: Sequence file $(basename "$seqFileB") does not have exactly two columns." >&2 - exit 1 - fi -done < "$seqFileB" - -outputLines=("${linesFileA[@]}" "${linesFileB[@]}") - -secondColumn=() -for line in "${outputLines[@]}"; do - columns=($line) - secondColumn+=("${columns[1]}") -done - -uniqueSecondColumn=($(echo "${secondColumn[@]}" | tr ' ' '\n' | sort -u)) -if [[ ${#secondColumn[@]} -ne ${#uniqueSecondColumn[@]} ]]; then - echo "Error: Duplicate sequence labels detected in second column for pair: $(basename "$seqFileA"), $(basename "$seqFileB")" >&2 - exit 1 -fi diff --git a/cleanNXF.sh b/cleanNXF.sh deleted file mode 100755 index 8c64a3e0..00000000 --- a/cleanNXF.sh +++ /dev/null @@ -1,11 +0,0 @@ -#!/usr/bin/env bash - -rm -rf .nextflow* -echo "Cleaned .nextflow..." -rm -rf .nextflow.pid -echo "Cleaned .nextflow.pid..." -for i in $(ls work | grep -v "conda"); -do - rm -rf "work/$i" -done -echo "Cleaned work..." diff --git a/conf/base.config b/conf/base.config index ab5a46a2..fad3aa91 100644 --- a/conf/base.config +++ b/conf/base.config @@ -1,119 +1,65 @@ -profiles { - local { - process { - executor = 'local' - } - } - - pfr { - process { - executor = 'slurm' - } - - apptainer { - envWhitelist= "APPTAINER_BINDPATH,APPTAINER_BIND" - } - } - - apptainer { - apptainer.enabled = true - apptainer.autoMounts= true - } - - docker { - docker.enabled = true - docker.runOptions = '--platform=linux/amd64' - } -} +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + plant-food-research-open/assemblyqc Nextflow base config file +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + A 'blank slate' config file, appropriate for general use on most high performance + compute environments. Assumes that all software is installed and available on + the PATH. Runs in `local` mode - all jobs will be run on the logged in environment. +---------------------------------------------------------------------------------------- +*/ -// Source: https://github.com/nf-core/rnaseq -// License: https://github.com/nf-core/rnaseq/blob/master/LICENSE process { - cpus = { check_max( 1 * task.attempt, 'cpus' ) } - memory = { check_max( 6.GB * task.attempt, 'memory' ) } - time = { check_max( 4.h * task.attempt, 'time' ) } + // TODO nf-core: Check the defaults for all processes + cpus = { check_max( 1 * task.attempt, 'cpus' ) } + memory = { check_max( 6.GB * task.attempt, 'memory' ) } + time = { check_max( 4.h * task.attempt, 'time' ) } - errorStrategy = { task.exitStatus in [140,143,137,104,134,139] ? 'retry' : 'finish' } - maxRetries = 1 - maxErrors = '-1' + errorStrategy = { task.exitStatus in ((130..145) + 104) ? 'retry' : 'finish' } + maxRetries = 1 + maxErrors = '-1' // Process-specific resource requirements // NOTE - Please try and re-use the labels below as much as possible. // These labels are used and recognised by default in DSL2 files hosted on nf-core/modules. // If possible, it would be nice to keep the same label naming convention when // adding in your local modules too. + // TODO nf-core: Customise requirements for specific processes. // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors withLabel:process_single { - cpus = { check_max( 1 , 'cpus' ) } - memory = { check_max( 6.GB * task.attempt, 'memory' ) } - time = { check_max( 4.h * task.attempt, 'time' ) } + cpus = { check_max( 1 , 'cpus' ) } + memory = { check_max( 6.GB * task.attempt, 'memory' ) } + time = { check_max( 4.h * task.attempt, 'time' ) } } withLabel:process_low { - cpus = { check_max( 2 * task.attempt, 'cpus' ) } - memory = { check_max( 12.GB * task.attempt, 'memory' ) } - time = { check_max( 4.h * task.attempt, 'time' ) } + cpus = { check_max( 2 * task.attempt, 'cpus' ) } + memory = { check_max( 12.GB * task.attempt, 'memory' ) } + time = { check_max( 4.h * task.attempt, 'time' ) } } withLabel:process_medium { - cpus = { check_max( 6 * task.attempt, 'cpus' ) } - memory = { check_max( 36.GB * task.attempt, 'memory' ) } - time = { check_max( 8.h * task.attempt, 'time' ) } + cpus = { check_max( 6 * task.attempt, 'cpus' ) } + memory = { check_max( 36.GB * task.attempt, 'memory' ) } + time = { check_max( 8.h * task.attempt, 'time' ) } } withLabel:process_high { - cpus = { check_max( 12 * task.attempt, 'cpus' ) } - memory = { check_max( 72.GB * task.attempt, 'memory' ) } - time = { check_max( 16.h * task.attempt, 'time' ) } + cpus = { check_max( 12 * task.attempt, 'cpus' ) } + memory = { check_max( 72.GB * task.attempt, 'memory' ) } + time = { check_max( 16.h * task.attempt, 'time' ) } } withLabel:process_long { - time = { check_max( 20.h * task.attempt, 'time' ) } - } - withLabel:process_two_days_long { - time = { check_max( 48.h * task.attempt, 'time' ) } - } - withLabel:process_week_long { - time = { check_max( 7.days * task.attempt, 'time' ) } + time = { check_max( 20.h * task.attempt, 'time' ) } } withLabel:process_high_memory { - memory = { check_max( 200.GB * task.attempt, 'memory' ) } + memory = { check_max( 200.GB * task.attempt, 'memory' ) } } - withLabel:process_very_high_memory { - memory = { check_max( 512.GB * task.attempt, 'memory' ) } + withLabel:error_ignore { + errorStrategy = 'ignore' } - - withName:CUSTOM_DUMPSOFTWAREVERSIONS { - cache = false + withLabel:error_retry { + errorStrategy = 'retry' + maxRetries = 2 } -} - -// Source: https://github.com/nf-core/rnaseq -// License: https://github.com/nf-core/rnaseq/blob/master/LICENSE -def check_max(obj, type) { - if (type == 'memory') { - try { - if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1) - return params.max_memory as nextflow.util.MemoryUnit - else - return obj - } catch (all) { - println " ### ERROR ### Max memory '${params.max_memory}' is not valid! Using default value: $obj" - return obj - } - } else if (type == 'time') { - try { - if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1) - return params.max_time as nextflow.util.Duration - else - return obj - } catch (all) { - println " ### ERROR ### Max time '${params.max_time}' is not valid! Using default value: $obj" - return obj - } - } else if (type == 'cpus') { - try { - return Math.min( obj, params.max_cpus as int ) - } catch (all) { - println " ### ERROR ### Max cpus '${params.max_cpus}' is not valid! Using default value: $obj" - return obj - } + withName:CUSTOM_DUMPSOFTWAREVERSIONS { + cache = false } } diff --git a/conf/igenomes.config b/conf/igenomes.config new file mode 100644 index 00000000..3f114377 --- /dev/null +++ b/conf/igenomes.config @@ -0,0 +1,440 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Nextflow config file for iGenomes paths +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Defines reference genomes using iGenome paths. + Can be used by any config that customises the base path using: + $params.igenomes_base / --igenomes_base +---------------------------------------------------------------------------------------- +*/ + +params { + // illumina iGenomes reference file paths + genomes { + 'GRCh37' { + fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt" + mito_name = "MT" + macs_gsize = "2.7e9" + blacklist = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed" + } + 'GRCh38' { + fasta = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "2.7e9" + blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" + } + 'CHM13' { + fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/BWAIndex/" + bwamem2 = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/BWAmem2Index/" + gtf = "${params.igenomes_base}/Homo_sapiens/NCBI/CHM13/Annotation/Genes/genes.gtf" + gff = "ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/009/914/755/GCF_009914755.1_T2T-CHM13v2.0/GCF_009914755.1_T2T-CHM13v2.0_genomic.gff.gz" + mito_name = "chrM" + } + 'GRCm38' { + fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt" + mito_name = "MT" + macs_gsize = "1.87e9" + blacklist = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed" + } + 'TAIR10' { + fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt" + mito_name = "Mt" + } + 'EB2' { + fasta = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt" + } + 'UMD3.1' { + fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt" + mito_name = "MT" + } + 'WBcel235' { + fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed" + mito_name = "MtDNA" + macs_gsize = "9e7" + } + 'CanFam3.1' { + fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt" + mito_name = "MT" + } + 'GRCz10' { + fasta = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed" + mito_name = "MT" + } + 'BDGP6' { + fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed" + mito_name = "M" + macs_gsize = "1.2e8" + } + 'EquCab2' { + fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt" + mito_name = "MT" + } + 'EB1' { + fasta = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt" + } + 'Galgal4' { + fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed" + mito_name = "MT" + } + 'Gm01' { + fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt" + } + 'Mmul_1' { + fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt" + mito_name = "MT" + } + 'IRGSP-1.0' { + fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed" + mito_name = "Mt" + } + 'CHIMP2.1.4' { + fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt" + mito_name = "MT" + } + 'Rnor_5.0' { + fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.bed" + mito_name = "MT" + } + 'Rnor_6.0' { + fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed" + mito_name = "MT" + } + 'R64-1-1' { + fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed" + mito_name = "MT" + macs_gsize = "1.2e7" + } + 'EF2' { + fasta = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt" + mito_name = "MT" + macs_gsize = "1.21e7" + } + 'Sbi1' { + fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt" + } + 'Sscrofa10.2' { + fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt" + mito_name = "MT" + } + 'AGPv3' { + fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed" + mito_name = "Mt" + } + 'hg38' { + fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "2.7e9" + blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" + } + 'hg19' { + fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "2.7e9" + blacklist = "${projectDir}/assets/blacklists/hg19-blacklist.bed" + } + 'mm10' { + fasta = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "1.87e9" + blacklist = "${projectDir}/assets/blacklists/mm10-blacklist.bed" + } + 'bosTau8' { + fasta = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed" + mito_name = "chrM" + } + 'ce10' { + fasta = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "9e7" + } + 'canFam3' { + fasta = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt" + mito_name = "chrM" + } + 'danRer10' { + fasta = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "1.37e9" + } + 'dm6' { + fasta = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "1.2e8" + } + 'equCab2' { + fasta = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt" + mito_name = "chrM" + } + 'galGal4' { + fasta = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt" + mito_name = "chrM" + } + 'panTro4' { + fasta = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt" + mito_name = "chrM" + } + 'rn6' { + fasta = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed" + mito_name = "chrM" + } + 'sacCer3' { + fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/" + readme = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "1.2e7" + } + 'susScr3' { + fasta = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/version0.6.0/" + bowtie2 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt" + mito_name = "chrM" + } + } +} diff --git a/conf/modules.config b/conf/modules.config index 11aa15b3..d91c6aba 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -1,220 +1,50 @@ -process { - withName: ASSEMBLATHON_STATS { - publishDir = [ - path: { "${params.outdir}/assemblathon_stats" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } - ] - } - - withName: NCBI_FCS_ADAPTOR { - publishDir = [ - path: { "${params.outdir}/ncbi_fcs_adaptor" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } - ] - } - - withName: RUN_BUSCO { - publishDir = [ - path: { "${params.outdir}/busco" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } - ] - } - - withName: RUN_KRAKEN2 { - publishDir = [ - path: { "${params.outdir}/kraken2" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } - ] - } - - withName: NCBI_FCS_GX_SCREEN_SAMPLES { - publishDir = [ - path: { "${params.outdir}/ncbi_fcs_gx" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } - ] - } - - withName: CIRCOS { - publishDir = [ - path: { "${params.outdir}/synteny/${target_on_ref_seq}" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } - ] - } -} - - -process { - withName: '.*:GFF3_VALIDATE:GT_GFF3' { - ext.args = '-tidy -retainids' - } - - withName: GT_STAT { - ext.args = [ - '-genelengthdistri', - '-genescoredistri', - '-exonlengthdistri', - '-exonnumberdistri', - '-intronlengthdistri', - '-cdslengthdistri', - '-addintrons' - ].join(' ').trim() - - publishDir = [ - path: { "${params.outdir}/genometools_gt_stat" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } - ] - } -} - -process { - - withName: FILTER_BY_LENGTH { - ext.args = params.tidk.filter_by_size ? "-m ${params.tidk.filter_size_bp}" : '' - ext.prefix = { "${meta.id}.filtered" } - } - - withName: SORT_BY_LENGTH { - ext.args = '--quiet --reverse --by-length' - ext.prefix = { "${meta.id}.sorted" } - } - - withName: TIDK_EXPLORE { - ext.args = '--minimum 5 --maximum 30' - publishDir = [ - path: { "${params.outdir}/tidk" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } - ] - } - - withName: TIDK_SEARCH_APRIORI { - ext.prefix = { "${meta.id}.apriori" } - ext.args = '--extension tsv' - publishDir = [ - path: { "${params.outdir}/tidk" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } - ] - } - - withName: TIDK_SEARCH_APOSTERIORI { - ext.prefix = { "${meta.id}.aposteriori" } - ext.args = '--extension tsv' - publishDir = [ - path: { "${params.outdir}/tidk" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } - ] - } - - withName: TIDK_PLOT_APRIORI { - ext.prefix = { "${meta.id}.apriori" } - publishDir = [ - path: { "${params.outdir}/tidk" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } - ] - } - - withName: TIDK_PLOT_APOSTERIORI { - ext.prefix = { "${meta.id}.aposteriori" } - publishDir = [ - path: { "${params.outdir}/tidk" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } - ] - } -} - +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Config file for defining DSL2 per module options and publishing paths +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Available keys to override module options: + ext.args = Additional arguments appended to command in module. + ext.args2 = Second set of arguments appended to command in module (multi-tool modules). + ext.args3 = Third set of arguments appended to command in module (multi-tool modules). + ext.prefix = File name prefix for output files. +---------------------------------------------------------------------------------------- +*/ process { - withName: CUSTOM_SHORTENFASTAIDS { - publishDir = [ - path: { "${params.outdir}/lai" }, - mode: "copy", - pattern: '*.short.ids.tsv' - ] - } - - withName: EDTA_LTRHARVEST { - ext.prefix = { "${meta.id}_edta_ltrharvest" } - } - - withName: LTRFINDER { - ext.args = '-harvest_out -size 1000000 -time 300' - } - - withName: CAT_CAT { - ext.prefix = { "${meta.id}_ltrharvest_ltrfinder.tabout" } - } - - withName: LTRRETRIEVER { - publishDir = [ - path: { "${params.outdir}/lai" }, - mode: "copy", - pattern: '*.LTRlib.fa' - ] - } - - withName: CUSTOM_RESTOREGFFIDS { - publishDir = [ - path: { "${params.outdir}/lai" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } - ] - } + publishDir = [ + path: { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] - withName: LAI { + withName: SAMPLESHEET_CHECK { publishDir = [ - path: { "${params.outdir}/lai" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + path: { "${params.outdir}/pipeline_info" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] } -} - -process { - withName: FASTQC_RAW { - publishDir = [ - path: { "${params.outdir}/hic/fastqc_raw" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } - ] + withName: FASTQC { + ext.args = '--quiet' } - withName: FASTQC_TRIM { + withName: CUSTOM_DUMPSOFTWAREVERSIONS { publishDir = [ - path: { "${params.outdir}/hic/fastqc_trim" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + path: { "${params.outdir}/pipeline_info" }, + mode: params.publish_dir_mode, + pattern: '*_versions.yml' ] } - withName: FASTP { - ext.args = params.hic.fastp_ext_args + withName: 'MULTIQC' { + ext.args = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' } publishDir = [ - path: { "${params.outdir}/hic/fastp" }, - mode: "copy", - saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + path: { "${params.outdir}/multiqc" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] } - withName: BWA_MEM { - ext.prefix = { "${meta.id}.on.${meta.ref_id}.bwa.mem" } - ext.args = '-5SP' - } - - withName: SAMBLASTER { - ext.prefix = { "${meta.id}.on.${meta.ref_id}.samblaster" } - ext.args3 = '-h -F 2316' - } } diff --git a/conf/reporting_defaults.config b/conf/reporting_defaults.config deleted file mode 100644 index 33542f0a..00000000 --- a/conf/reporting_defaults.config +++ /dev/null @@ -1,15 +0,0 @@ -// Source: https://github.com/nf-core/rnaseq -// License: https://github.com/nf-core/rnaseq/blob/master/LICENSE -def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') -timeline { - enabled = true - file = "${params.outdir}/pipeline_info/execution_timeline_${trace_timestamp}.html" -} -report { - enabled = true - file = "${params.outdir}/pipeline_info/execution_report_${trace_timestamp}.html" -} -trace { - enabled = true - file = "${params.outdir}/pipeline_info/execution_trace_${trace_timestamp}.txt" -} diff --git a/conf/test.config b/conf/test.config new file mode 100644 index 00000000..2bc1ad8e --- /dev/null +++ b/conf/test.config @@ -0,0 +1,29 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Nextflow config file for running minimal tests +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Defines input files and everything required to run a fast and simple pipeline test. + + Use as follows: + nextflow run plant-food-research-open/assemblyqc -profile test, --outdir + +---------------------------------------------------------------------------------------- +*/ + +params { + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test dataset to check pipeline function' + + // Limit resources so that this can run on GitHub Actions + max_cpus = 2 + max_memory = '6.GB' + max_time = '6.h' + + // Input data + // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets + // TODO nf-core: Give any required params for the test so that command line flags are not needed + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' + + // Genome references + genome = 'R64-1-1' +} diff --git a/conf/test_bacterial.config b/conf/test_bacterial.config deleted file mode 100644 index 558e53f2..00000000 --- a/conf/test_bacterial.config +++ /dev/null @@ -1,64 +0,0 @@ -params { - - // Vibrio parahaemolyticus: https://www.ncbi.nlm.nih.gov/datasets/genome/GCF_000196095.1/ - target_assemblies = [ - [ - "v_parahaemolyticus", - "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/196/095/GCF_000196095.1_ASM19609v1/GCF_000196095.1_ASM19609v1_genomic.fna.gz" - ], - ] - - assembly_gff3 = [ - [ - "v_parahaemolyticus", - "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/196/095/GCF_000196095.1_ASM19609v1/GCF_000196095.1_ASM19609v1_genomic.gff.gz" - ], - ] - - assemblathon_stats { - n_limit = 100 - } - - ncbi_fcs_adaptor { - empire = 'prok' - } - - ncbi_fcs_gx { - tax_id = "670" - db_path = "/workspace/ComparativeDataSources/NCBI/FCS/GX/r2023-01-24" - } - - busco { - mode = "geno" - lineage_datasets = ["bacteria_odb10", "gammaproteobacteria_odb10", "vibrionales_odb10"] - download_path = "/workspace/ComparativeDataSources/BUSCO/assembly_qc" - } - - tidk { - skip = 1 - } - - lai { - monoploid_seqs = [] - } - - kraken2 { - db_path = "/workspace/ComparativeDataSources/kraken2db/k2_pluspfp_20230314" - } - - hic { - skip = 1 - } - - synteny { - skip = 1 - } - - outdir = "./results" - - max_time = 6.hours -} - -apptainer { - cacheDir = "/workspace/assembly_qc/singularity" -} diff --git a/conf/test_full.config b/conf/test_full.config index d06036b3..63d2687c 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -1,79 +1,24 @@ -params { - - target_assemblies = [ - [ - "FI1", - "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz" - ], - ] - - assembly_gff3 = [ - [ - "FI1", - "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz" - ], - ] - - assemblathon_stats { - n_limit = 100 - } - - ncbi_fcs_adaptor { - empire = 'euk' - } - - ncbi_fcs_gx { - tax_id = "35717" - db_path = "/workspace/ComparativeDataSources/NCBI/FCS/GX/r2023-01-24" - } - - busco { - mode = "geno" - lineage_datasets = ["fungi_odb10", "hypocreales_odb10"] - download_path = "/workspace/ComparativeDataSources/BUSCO/assembly_qc" - } - - tidk { - repeat_seq = "TTAGGG" - } +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Nextflow config file for running full-size tests +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Defines input files and everything required to run a full size pipeline test. - lai { - monoploid_seqs = [ - ["FI1", "./docs/test_files/FI1.monoploid.seqs.txt"] - ] - } + Use as follows: + nextflow run plant-food-research-open/assemblyqc -profile test_full, --outdir - kraken2 { - db_path = "/workspace/ComparativeDataSources/kraken2db/k2_pluspfp_20230314" - } +---------------------------------------------------------------------------------------- +*/ - hic { - paired_reads = "SRR8238190" - - skip_fastp = 0 - skip_fastqc = 0 - } - - synteny { - - assembly_seq_list = [ - ["FI1", "./docs/test_files/FI1.seq.list"] - ] - - xref_assemblies = [ - [ - "TT_2021a", - "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/021/950/295/GCA_021950295.1_ASM2195029v1/GCA_021950295.1_ASM2195029v1_genomic.fna.gz", - "./docs/test_files/TT_2021a.seq.list" - ], - ] - } - - outdir = "./results" +params { + config_profile_name = 'Full test profile' + config_profile_description = 'Full test dataset to check pipeline function' - max_time = 6.hours -} + // Input data for full size test + // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) + // TODO nf-core: Give any required params for the test so that command line flags are not needed + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' -apptainer { - cacheDir = "/workspace/assembly_qc/singularity" + // Genome references + genome = 'R64-1-1' } diff --git a/conf/test_invalid.config b/conf/test_invalid.config deleted file mode 100644 index 6af810e6..00000000 --- a/conf/test_invalid.config +++ /dev/null @@ -1,40 +0,0 @@ -params { - target_assemblies = [ - [ - "FI1", - "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz" - ], - [ - "FI2", - "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz" - ] - ] - - assembly_gff3 = [ - [ - "FI1", - "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz" - ], - [ - "FI2", - "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/196/095/GCF_000196095.1_ASM19609v1/GCF_000196095.1_ASM19609v1_genomic.gff.gz" - ], - ] - - ncbi_fcs_adaptor { skip = 1 } - ncbi_fcs_gx { skip = 1 } - busco { skip = 1 } - tidk { skip = 1 } - lai { skip = 1 } - kraken2 { skip = 1 } - hic { skip = 1 } - synteny { skip = 1 } - - outdir = "./results" - - max_time = 1.hour -} - -apptainer { - cacheDir = "/workspace/assembly_qc/singularity" -} diff --git a/conf/test_minimal.config b/conf/test_minimal.config deleted file mode 100644 index f1dda81e..00000000 --- a/conf/test_minimal.config +++ /dev/null @@ -1,32 +0,0 @@ -params { - target_assemblies = [ - [ - "FI1", - "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz" - ], - ] - - assembly_gff3 = [ - [ - "FI1", - "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz" - ], - ] - - ncbi_fcs_adaptor { skip = 1 } - ncbi_fcs_gx { skip = 1 } - busco { skip = 1 } - tidk { skip = 1 } - lai { skip = 1 } - kraken2 { skip = 1 } - hic { skip = 1 } - synteny { skip = 1 } - - outdir = "./results" - - max_time = 1.hour -} - -apptainer { - cacheDir = "/workspace/assembly_qc/singularity" -} diff --git a/conf/test_transcriptome.config b/conf/test_transcriptome.config deleted file mode 100644 index bb8d1d61..00000000 --- a/conf/test_transcriptome.config +++ /dev/null @@ -1,55 +0,0 @@ -params { - - // Mesorhabditis belari (nematodes) transcriptome: https://www.ncbi.nlm.nih.gov/assembly/GCA_900631935.1 - target_assemblies = [ - [ - "m_belari", - "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/900/631/935/GCA_900631935.1_M.Belari_Hybrid_transcriptome/GCA_900631935.1_M.Belari_Hybrid_transcriptome_genomic.fna.gz" - ], - ] - - assembly_gff3 = [] - - ncbi_fcs_adaptor { - empire = 'euk' - } - - ncbi_fcs_gx { - tax_id = "2138241" - db_path = "/workspace/ComparativeDataSources/NCBI/FCS/GX/r2023-01-24" - } - - busco { - mode = "tran" - lineage_datasets = ["eukaryota_odb10", "metazoa_odb10", "nematoda_odb10"] - download_path = "/workspace/ComparativeDataSources/BUSCO/assembly_qc" - } - - tidk { - skip = 1 - } - - lai { - skip = 1 - } - - kraken2 { - db_path = "/workspace/ComparativeDataSources/kraken2db/k2_pluspfp_20230314" - } - - hic { - skip = 1 - } - - synteny { - skip = 1 - } - - outdir = "./results" - - max_time = 6.hours -} - -apptainer { - cacheDir = "/workspace/assembly_qc/singularity" -} diff --git a/conf/test_viral.config b/conf/test_viral.config deleted file mode 100644 index b02d3759..00000000 --- a/conf/test_viral.config +++ /dev/null @@ -1,58 +0,0 @@ -params { - - // HIV1: https://www.ncbi.nlm.nih.gov/datasets/genome/GCF_000864765.1 - target_assemblies = [ - [ - "hiv", - "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/864/765/GCF_000864765.1_ViralProj15476/GCF_000864765.1_ViralProj15476_genomic.fna.gz" - ], - ] - - assembly_gff3 = [ - [ - "hiv", - "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/864/765/GCF_000864765.1_ViralProj15476/GCF_000864765.1_ViralProj15476_genomic.gff.gz" - ], - ] - - ncbi_fcs_adaptor { - empire = 'prok' - } - - ncbi_fcs_gx { - tax_id = "11676" - db_path = "/workspace/ComparativeDataSources/NCBI/FCS/GX/r2023-01-24" - } - - busco { - skip = 1 - } - - tidk { - skip = 1 - } - - lai { - skip = 1 - } - - kraken2 { - db_path = "/workspace/ComparativeDataSources/kraken2db/k2_pluspfp_20230314" - } - - hic { - skip = 1 - } - - synteny { - skip = 1 - } - - outdir = "./results" - - max_time = 6.hours -} - -apptainer { - cacheDir = "/workspace/assembly_qc/singularity" -} diff --git a/docs/README.md b/docs/README.md index 25dbf8f0..20428bd5 100644 --- a/docs/README.md +++ b/docs/README.md @@ -1,14 +1,8 @@ -# AssemblyQC Tutorials +# plant-food-research-open/assemblyqc: Documentation -1. [Quick Start: A Minimal Example](./minimal_example.md) -2. [Configuring the Pipeline for a Complete Run](./full_run.md) -3. [Execution with Amazon Genomics CLI](./aws_run.md) +The plant-food-research-open/assemblyqc documentation is split into the following pages: -## Test Configurations - -The following test configurations are included with the pipeline. Before going through these configurations, - -- [Test Configuration for a Fungus](../conf/test_full.config) -- [Test Configuration for a Bacterium](../conf/test_bacterial.config) -- [Test Configuration for a Virus](../conf/test_viral.config) -- [Test Configuration for a Transcriptome of a Nematode](../conf/test_viral.config) +- [Usage](usage.md) + - An overview of how the pipeline works, how to run it and a description of all of the different command-line flags. +- [Output](output.md) + - An overview of the different results produced by the pipeline and how to interpret them. diff --git a/docs/aws_run.md b/docs/aws_run.md deleted file mode 100644 index 53a96b44..00000000 --- a/docs/aws_run.md +++ /dev/null @@ -1,18 +0,0 @@ -# Execution with Amazon Genomics CLI - -The pipeline can be executed on AWS Batch using the Amazon Genomics CLI (AGC). Please first go through the [AGC examples](https://catalog.workshops.aws/agc-pipelines/en-US/02-running-pipelines/02-nextflow) before continuing this tutorial. - -An AGC project configured for the pipeline is included with the source code, [agc-project.yaml](../agc-project.yaml). An example parameters file with test data is also included, [test_agc.json](../test_params/test_agc.json). Some of the parameters in this file are configured to take data from a private bucket. These parameters must be redirected to a bucket accessible to you. These parameters are: - -- ncbi_fcs_gx::db_path -- kraken2::db_path -- outdir - -> [!WARNING] -> The location specified by `outdir` should be changed when the dataset is changed. Otherwise, the pipeline will overwrite the existing files in this directory. - -After creating a valid parameters file, replace the path of the existing parameters file with your parameters file path in the `inputFileURLs` in [MANIFEST.json](../MANIFEST.json). Next, the pipeline can be submitted to AWS for execution. - -```bash -agc workflow run PFR_ASSEMBLY_QC -c CtxAssemblyQC -v -``` diff --git a/docs/full_run.md b/docs/full_run.md deleted file mode 100644 index c9c46e57..00000000 --- a/docs/full_run.md +++ /dev/null @@ -1,242 +0,0 @@ -# Configuring the Pipeline for a Complete Run - -This document explains how to correctly configure the pipeline for a complete run. For a minimal example which documents basic configuration along with pipeline execution, refer to [Quick Start: A Minimal Example](./minimal_example.md). - -- [Configuring the Pipeline for a Complete Run](#configuring-the-pipeline-for-a-complete-run) - - [Complete Example Configuration File](#complete-example-configuration-file) - - [ASSEMBLATHON STATS](#assemblathon-stats) - - [NCBI FCS Adaptor](#ncbi-fcs-adaptor) - - [NCBI FCS GX](#ncbi-fcs-gx) - - [BUSCO](#busco) - - [TIDK](#tidk) - - [LAI](#lai) - - [KRAKEN2](#kraken2) - - [HIC](#hic) - - [SYNTENY](#synteny) - -## Complete Example Configuration File - -This document explains the pipeline configuration using an example configuration file packaged with the pipeline. Refer to 'conf/test_full.config'. This configuration is an expansion of the 'conf/test_minimal.config' covered in [Quick Start: A Minimal Example](./minimal_example.md). - -## ASSEMBLATHON STATS - -There is only one configurable parameter for this module: `n_limit`. This is the number of 'N's for the unknown gap size. This number is used to split the scaffolds into contigs to compute contig-related stats. NCBI's recommendation for unknown gap size is 100 . - -> ⚙️ From conf/test_full.config - -```groovy -assemblathon_stats { - n_limit = 100 -} -``` - -## NCBI FCS Adaptor - -This module has only one parameter: `empire`. The permissible values are: `euk` for Eukaryotes and `prok` for Prokaryotes. - -> ⚙️ From conf/test_full.config - -```groovy -ncbi_fcs_adaptor { - empire = 'euk' -} -``` - -## NCBI FCS GX - -Following parameters must be configured: - -- `tax_id`: The taxonomy ID for all the target assemblies listed in the `target_assemblies` parameter. A taxonomy ID can be obtained by searching a _Genus species_ at . A single ID for all assemblies implies that the pipeline is designed to be used for checking one or more assemblies of the same _species_ in one run. -- `db_path`: This is the path to the database files stored on a directory accessible to the pipeline. Before running the pipeline, the user must ensure that the database is correctly downloaded and placed in a directory accessible to the pipeline. Setup instructions are available at . The database directory should contain following files: - -```bash -all.assemblies.tsv -all.blast_div.tsv.gz -all.gxi -all.gxs -all.manifest -all.meta.jsonl -all.README.txt -all.seq_info.tsv.gz -all.taxa.tsv -``` - -> ⚙️ From conf/test_full.config - -```groovy -ncbi_fcs_gx { - tax_id = "35717" - db_path = "/workspace/ComparativeDataSources/NCBI/FCS/GX/r2023-01-24" -} -``` - -## BUSCO - -Following parameters must be configured: - -- `mode`: geno or genome, for genome assemblies (DNA), tran or transcriptome, for transcriptome assemblies (DNA); and prot or proteins, for annotated gene sets (protein). -- `lineage_datasets`: A list of BUSCO lineages. Any number of lineages can be specified. Each target assembly is assessed against each of the listed lineage. To select a lineage, refer to -- `download_path`: A directory where the BUSCO can download and cache its databases. BUSCO manages download and validation of the databases itself, therefore, the user does not have to manually setup these databases. If this is set to `null`, NextFLow will download the database in the process work directory. - -> ⚙️ From conf/test_full.config - -```groovy -busco { - mode = "geno" - lineage_datasets = ["fungi_odb10", "hypocreales_odb10"] - download_path = "/workspace/ComparativeDataSources/BUSCO/assembly_qc" -} -``` - -## TIDK - -Following parameter must be configured: - -- `repeat_seq`: The telomere search sequence. To select an appropriate sequence, see . Commonly used sequences are: TTTAGGG (Plant), TTAGGG (Fungus, Vertebrates), TTAGG (Insect). - -The following parameters are optional: - -- `filter_by_size`: Set this flag to 1 to filter out assembly sequences smaller than the size specified by the next parameter (default: 0). -- `filter_size_bp`: Minimum size of the assembly sequence processed by TIDK (default: 1000000 (1Mbp)). - -> ⚙️ From conf/test_full.config - -```groovy -tidk { - repeat_seq = "TTAGGG" -} -``` - -In the example configuration above, the `filter_by_size` and `filter_size_bp` are not set. The pipeline will pick up their default values from 'nextflow.config' file. - -## LAI - -Following parameter must be configured: - -- `monoploid_seqs`: A list of lists which specifies the `-mono` parameter-file for LAI when processing a polyploid assembly. The `-mono` parameter-file is a single column text file listing IDs of the monoploid sequences for a polyploid assembly. If this parameter is not needed, it can be set to `[]`. If only some of the assemblies listed in `target_assemblies` are polyploid, the `-mono` parameter-file can be specified only for those assemblies. Similar to the `pass_list` parameter, an assembly is identified by its tag. Here are the contents of an example `-mono` parameter-file: - -```TSV -CP031385.1 -CP031386.1 -CP031387.1 -CP031388.1 -CP031389.1 -CP031390.1 -CP031391.1 -``` - -> ⚙️ From conf/test_full.config - -```groovy -lai { - monoploid_seqs = [ - ["FI1", "./docs/test_files/FI1.monoploid.seqs.txt"] - ] -} -``` - -## KRAKEN2 - -Following parameters must be configured: - -- `db_path`: Path to a directory or a `*.tar.gz` file containing the Kraken2 database. - -> ⚙️ From conf/test_full.config - -```groovy -kraken2 { - db_path = "/workspace/ComparativeDataSources/kraken2db/k2_pluspfp_20230314" -} -``` - -## HIC - -Following parameter must be configured: - -- `paired_reads`: A relative or absolute path to paired reads in fastq.gz format, or a SRA ID. The format for file path is `*R{1,2}*.(fasta|fq).gz`. An example is '/input/genomic/fungal/Neonectria/Genome/20190506_CAGRF19591_CGYCF_HiC/PG_PETUNIA_HiC_CGYCF_CACTCA_L001_R{1,2}.fastq.gz'. -- `skip_fastp`: Skips fastp trimming. -- `skip_fastqc`: Skips QC by fastqc. -- `fastp_ext_args`: Additional arguments for fastp. - -> ⚙️ From conf/test_full.config - -```groovy -hic { - paired_reads = "SRR8238190" - - skip_fastp = 0 - skip_fastqc = 0 - - fastp_ext_args = '--qualified_quality_phred 20 --length_required 50' -} -``` - -## SYNTENY - -Following parameters must be configured: - -- `assembly_seq_list`: This is a list of lists which specifies the `*.seq.list` file for each assembly declared in the `target_assemblies` parameter. A `*.seq.list` file is a two column tab-delimited txt file listing fasta sequence ids (first column) and labels for the synteny plots (second column). An example file is shown below: - -```TSV -CP031385.1 FI1_1 -CP031386.1 FI1_2 -CP031387.1 FI1_3 -CP031388.1 FI1_4 -CP031389.1 FI1_5 -CP031390.1 FI1_6 -CP031391.1 FI1_7 -``` - -This parameter is specified as a list of lists. Each sub-list has two elements. The first element identifies the assembly `target_assemblies` using the assembly tag. The second element is the path to the `*.seq.list` file. Here is an example: - -```groovy -target_assemblies = [ - ["hap1", "/workspace/assembly_qc/test_data/default/test_data1.fasta.gz"], - ["hap2", "/workspace/assembly_qc/test_data/default/test_data2.fasta"] -] - -assembly_seq_list = [ - ["hap1", "/workspace/assembly_qc/test_data/default/test_data1.seq.list"], - ["hap2", "/workspace/assembly_qc/test_data/default/test_data2.seq.list"] -] -``` - -- `xref_assemblies`: A list of lists which specifies reference assemblies against which the synteny should be performed. This parameter can be set to an empty list `[]` if reference assemblies are not available. To specify a reference assembly, three items must be declared. First a unique tag for the reference assembly, second a fasta file (fasta, fasta.gz) for the assembly, and, third, a `*.seq.list` file. Here is an example: - -```groovy -xref_assemblies = [ - [ - "GenomeA", - "/workspace/assembly_qc/test_data/default/test_data3.fasta", - "/workspace/assembly_qc/test_data/default/test_data3.seq.list" - ] -] -``` - -The following parameters are optional: - -- `between_target_asm`: Set it to 1 to create syntenic plots between each pair of target_assemblies. Default is 1. This parameter is useful if multiple assemblies are specified by the `target_assemblies` parameter and the user needs control over whether syntenic plots are created between each pair of assemblies or not. -- `many_to_many_align`: Set it to 1 to include alignment blocks with many-to-many mappings or set to 0 to only include 1-to-1 mappings. Default is 0. See the documentation of `dnadiff` for further details: -- `max_gap`: Alignments within this distance are bundled together. Default: 1000000 (1 Mbp). -- `min_bundle_size`: After bundling, any bundle smaller than this size is filtered out. Default: 1000 (1 Kbp). -- `plot_1_vs_all`: Set it to 1 to create a separate synteny plot for each contig of the target assembly versus all contigs of the reference assembly. Set it to 0 to create a single plot for each target assembly against each reference assembly. This joint plot is also created when `plot_1_vs_all` is set to 1. Default: 0. -- `color_by_contig`: Set it to 1 to color the synteny plot by contig. Set it to 0 to color the synteny plot by the number of links in a bundle. Default: 1. - -> ⚙️ From conf/test_full.config - -```groovy -synteny { - - assembly_seq_list = [ - ["FI1", "./docs/test_files/FI1.seq.list"] - ] - - xref_assemblies = [ - [ - "TT_2021a", - "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/021/950/295/GCA_021950295.1_ASM2195029v1/GCA_021950295.1_ASM2195029v1_genomic.fna.gz", - "./docs/test_files/TT_2021a.seq.list" - ], - ] -} -``` diff --git a/docs/images/mqc_fastqc_adapter.png b/docs/images/mqc_fastqc_adapter.png new file mode 100755 index 0000000000000000000000000000000000000000..361d0e47acfb424dea1f326590d1eb2f6dfa26b5 GIT binary patch literal 23458 zcmeFZ2UJtryD!S#x<#o93es(Ww4k)maRbte0-+a?-g^xY-3myTE`8G_KvA54)F1tn})nJ5u%TA4Y;^!^{48eL_}p#q-Umo0M|F1 z74+PQh^X8N|9_jcWbq~ zzn+tZC9B75nKdz=gQ8wo9GJ$P{D~3knlI_`-PRhCw34f1oYDLr^;oEbgxa#A^J%*2 z>FfDE*(~JzKFs$t_oeLz))qDU?s}%Q?7b~3Y;lUi^Oy-2@3g?joA4Wkgb6-2=ih*jub)~7yZ`T=L=Z`B`{1jhkB-iSjea94&Eo9A zxN59pv1p_}RO1>EC^q}Z2)ZI;b7JV_x4lMr=Bker2+EK;8~!;JO7re*@ZkDmoV878S*N^yX(F@U1yqt?Is3nnV>7}#(5pk`V3C) 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- [Step 0: System Prerequisites](#step-0-system-prerequisites) - - [Step 1: Setting up the Data](#step-1-setting-up-the-data) - - [Fasta Files](#fasta-files) - - [Gene Annotations (Optional)](#gene-annotations-optional) - - [Step 2: Skipping Optional Modules](#step-2-skipping-optional-modules) - - [Step 3: Setting Max. Resources](#step-3-setting-max-resources) - - [Step 4a: Setting the Apptainer Cache Directory](#step-4a-setting-the-apptainer-cache-directory) - - [Step 4b: Setting up Docker](#step-4b-setting-up-docker) - - [Example Minimal Config File](#example-minimal-config-file) - - [Step 5: Running the Pipeline](#step-5-running-the-pipeline) - - [Running on Plant\&Food Research Slurm](#running-on-plantfood-research-slurm) - - [Running on a Single Machine](#running-on-a-single-machine) - - [Running on Executors other than Slurm](#running-on-executors-other-than-slurm) - - [AssemblyQC Report](#assemblyqc-report) - -## Step 0: System Prerequisites - -1. A single computer with linux or a linux-based compute cluster. -2. NextFlow >= 22.04.3 -3. Apptainer or Docker - -## Step 1: Setting up the Data - -The pipeline can QC multiple assemblies in parallel. All these assemblies should be in fasta format. - -### Fasta Files - -The pipeline configuration is stored in the 'nextflow.config' file. In this file, add the fasta files (fasta, fasta.gz) to the `target_assemblies` variable under the `params` score. Here is an example: - -```groovy -target_assemblies = [ - ["assembly1", "./test_data/test_data1.fasta.gz"], - ["assembly2", "/output/genomes/test_genome/all_genomic.fsa"], - ["assembly3", "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz"] -] -``` - -Notice that `target_assemblies` is a list of lists. Each sub-list represents an assembly. Each sub-list must have two members. First, a unique tag that represents the assembly. This tag is used by the pipeline to identify this assembly across QC modules. This tag should only consist of alphanumeric characters (A-Za-z0-9\_). Second, the path to the fasta file (fasta, fasta.gz). This path can be a relative, absolute storage path or a valid publicly accessible URL. - -### Gene Annotations (Optional) - -If one or more of these assemblies have gene annotation files, these files should be in gff3 format (gff3, gff3.gz). These files are specified in the `assembly_gff3` parameter. The rules for specifying the gff3 files are same as those for the fasta files. Here is an example: - -```groovy -assembly_gff3 = [ - ["assembly2", "/output/genomes/test_genome/all_genes.gff3"] -] -``` - -Notice that only one of assemblies have annotation. This is a perfectly valid specification. If none of the assemblies has any annotation, the correct configuration is: - -```groovy -assembly_gff3 = [] -``` - -## Step 2: Skipping Optional Modules - -Some of the modules in the QC pipeline are optional. These modules can be skipped by setting their `skip` flag to `1`. These skip flags are found under the modules' configuration scopes under the `params` scope. If a module is skipped, all its remaining parameters are ignored by the pipeline. - -This minimal example sets all the skip flags to 1. - -## Step 3: Setting Max. Resources - -The resources needed for the various modules in the pipeline are dynamically allocated using resource-classes defined in the 'conf/base.config' file. Instead of tweaking these classes, the user can conveniently cap the maximum allowed resources by changing the `max_cpus`, `max_memory` and `max_time` variables in the 'nextflow.config' file. This example caps the maximum time to 1 hour as each module in this example can be executed within an hour. - -```groovy -max_time = 1.hour -``` - -> [!NOTE] -> Maximum values defined by `max_cpus`, `max_memory` and `max_time` apply to each process in the pipeline. The pipeline executes multiple processes in parallel. Therefore, the total execution time is not equal to the sum of time taken by each process. Rather, the total time is determined by adding up the time taken by processes which run one after the other. An estimate of the total time maybe needed if the pipeline is submitted to an executor such as Slurm. This topic is covered later in this document. - -## Step 4a: Setting the Apptainer Cache Directory - -> See [Step 4b](#step-4b-setting-up-docker) if you are using docker. - -The pipeline uses version controlled apptainer containers so that its results are reproducible across systems. These apptainer containers are automatically downloaded by the pipeline when it runs for the first time. The containers are then stored for later runs in the folder specified by the `cacheDir` parameter under the `apptainer` scope inside the 'nextflow.config' file. - -When downloading these containers, the pipeline can fail due to connection issues. In such a case, the pipeline should be resumed with the `-resume` flag. For more on the resume capability, see the NextFlow [documentation](https://www.nextflow.io/docs/latest/getstarted.html?highlight=resume#modify-and-resume). It may be a good idea to test run the pipeline with a small dataset so that it can download the necessary containers. Moreover, the `cacheDir` should not be changed afterwards. Otherwise, the pipeline will have to download the required containers again. - -## Step 4b: Setting up Docker - -If you are using docker, the docker daemon should be up and running. That's all. NextFlow will automatically handle container download and setup. - -## Example Minimal Config File - -An example minimal config file based on publicly accessible data is provided with the pipeline. See the 'conf/test_minimal.config' file in the project directory. Its contents are pasted here: - -```groovy -params { - target_assemblies = [ - [ - "FI1", - "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz" - ], - ] - - assembly_gff3 = [ - [ - "FI1", - "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz" - ], - ] - - ncbi_fcs_adaptor { skip = 1 } - ncbi_fcs_gx { skip = 1 } - busco { skip = 1 } - tidk { skip = 1 } - lai { skip = 1 } - kraken2 { skip = 1 } - hic { skip = 1 } - synteny { skip = 1 } - - outdir = "./results" - - max_time = 1.hour -} - -apptainer { - cacheDir = "/workspace/assembly_qc/singularity" -} -``` - -## Step 5: Running the Pipeline - -The next sections explain how to run the pipeline on Plant&Food Research Slurm cluster, a single machine or other executors. - -### Running on Plant&Food Research Slurm - -To submit the pipeline to Slurm for execution, first create a submission script with the following bash commands: - -```bash -cat << EOF > pfr_assemblyqc -#!/bin/bash -e - -#SBATCH --job-name ASM_QC -#SBATCH --time=01:00:00 -#SBATCH --nodes=1 -#SBATCH --ntasks=1 -#SBATCH --cpus-per-task=1 -#SBATCH --output pfr_assemblyqc.stdout -#SBATCH --error pfr_assemblyqc.stderr -#SBATCH --mem=4G - -ml unload perl -ml apptainer/1.1 -ml nextflow/23.04.4 - -export TMPDIR="/workspace/$USER/tmp" -export APPTAINER_BINDPATH="$APPTAINER_BINDPATH,$TMPDIR:$TMPDIR,$TMPDIR:/tmp" - -nextflow main.nf -profile pfr -resume -c ./conf/test_minimal.config -EOF -``` - -The overall time is specified as 1 hour. This is the time for which the NextFlow process is allowed to run by Slurm. This is an estimate of the total execution time and is based on the assumption that the parallel execution of all the processes in this minimal example can be completed within 1 hour. - -Similarly, the script specifies the number of CPUs and memory required for running NextFLow. These resources are only for running NextFlow and not the individual modules. Therefore, 1 CPU with 4 GBs of memory is adequate. - -The next 4 lines starting with ml specify the environment modules used by the pipeline. These names of these modules are system dependent. Refer to your system manuals to find out the modules which satisfy the requirements listed in [Step 0: System Prerequisites](#step-0-system-prerequisites). - -The `export TMPDIR` directory specifies the location of the temporary directory. This is system specific and should be specified by referring to the system manuals. - -The last line executes the pipeline implemented in the `main.nf` file with profile slurm and `-resume` flag. - -After creating the slurm submission script, submit to slurm as follows: - -```bash -sbatch ./pfr_assemblyqc -``` - -### Running on a Single Machine - -To run the pipeline on a single machine, make sure that the maximum resources specified by `max_cpus` and `max_memory` variables in the 'nextflow.config' file are suitable for your machine. Moreover, the minimum software required [Step 0: System Prerequisites](#step-0-system-prerequisites) should be available on the machine. Finally, the pipeline can be executed with the following command. - -```bash -nextflow main.nf -profile local,docker -resume -c ./conf/test_minimal.config -``` - -Notice that the `-profile` parameter is now set to `local,docker` in the NextFlow execution command. - -### Running on Executors other than Slurm - -To execute the pipeline on a executor other than Slurm, you will first have to create a profile for the target executor. See the existing profiles in the 'conf/base.config' file. Detailed documentation is available in NextFlow [docs](https://www.nextflow.io/docs/latest/executor.html) and nf-core [docs](https://nf-co.re/docs/usage/tutorials/step_by_step_institutional_profile). - -## AssemblyQC Report - -Once the pipeline has finished execution, the results folder specified in the config file should contain a file named 'report.html'. The 'report.html' is a standalone file for all the modules except HiC and Kraken2. Thus, if you move the report to another folder, make sure to also move the 'hic' folder and the 'kraken2' folder with it. diff --git a/docs/output.md b/docs/output.md new file mode 100644 index 00000000..dd6b3c21 --- /dev/null +++ b/docs/output.md @@ -0,0 +1,71 @@ +# plant-food-research-open/assemblyqc: Output + +## Introduction + +This document describes the output produced by the pipeline. Most of the plots are taken from the MultiQC report, which summarises results at the end of the pipeline. + +The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory. + + + +## Pipeline overview + +The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: + +- [FastQC](#fastqc) - Raw read QC +- [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline +- [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution + +### FastQC + +
+Output files + +- `fastqc/` + - `*_fastqc.html`: FastQC report containing quality metrics. + - `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images. + +
+ +[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). + +![MultiQC - FastQC sequence counts plot](images/mqc_fastqc_counts.png) + +![MultiQC - FastQC mean quality scores plot](images/mqc_fastqc_quality.png) + +![MultiQC - FastQC adapter content plot](images/mqc_fastqc_adapter.png) + +:::note +The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. +::: + +### MultiQC + +
+Output files + +- `multiqc/` + - `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser. + - `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline. + - `multiqc_plots/`: directory containing static images from the report in various formats. + +
+ +[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. + +Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see . + +### Pipeline information + +
+Output files + +- `pipeline_info/` + - Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`. + - Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline. + - Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`. + - Parameters used by the pipeline run: `params.json`. + +
+ +[Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage. diff --git a/docs/test_files/FI1.monoploid.seqs.txt b/docs/test_files/FI1.monoploid.seqs.txt deleted file mode 100644 index f54b5e6b..00000000 --- a/docs/test_files/FI1.monoploid.seqs.txt +++ /dev/null @@ -1,7 +0,0 @@ -CP031385.1 -CP031386.1 -CP031387.1 -CP031388.1 -CP031389.1 -CP031390.1 -CP031391.1 diff --git a/docs/test_files/FI1.seq.list b/docs/test_files/FI1.seq.list deleted file mode 100644 index 73636ff6..00000000 --- a/docs/test_files/FI1.seq.list +++ /dev/null @@ -1,7 +0,0 @@ -CP031385.1 FI1_1 -CP031386.1 FI1_2 -CP031387.1 FI1_3 -CP031388.1 FI1_4 -CP031389.1 FI1_5 -CP031390.1 FI1_6 -CP031391.1 FI1_7 diff --git a/docs/test_files/TT_2021a.seq.list b/docs/test_files/TT_2021a.seq.list deleted file mode 100644 index a51ee157..00000000 --- a/docs/test_files/TT_2021a.seq.list +++ /dev/null @@ -1,7 +0,0 @@ -CP083245.1 TT1 -CP083246.1 TT2 -CP083247.1 TT3 -CP083248.1 TT4 -CP083249.1 TT5 -CP083250.1 TT6 -CP083251.1 TT7 diff --git a/docs/test_params/test_agc.json b/docs/test_params/test_agc.json deleted file mode 100644 index 451a82ad..00000000 --- a/docs/test_params/test_agc.json +++ /dev/null @@ -1,65 +0,0 @@ -{ - "target_assemblies": [ - [ - "FI1", - "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz" - ] - ], - "assembly_gff3": [ - [ - "FI1", - "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz" - ] - ], - "assemblathon_stats": { - "n_limit": 100 - }, - "ncbi_fcs_adaptor": { - "empire": "euk" - }, - "ncbi_fcs_gx": { - "tax_id": "35717", - "db_path": "s3://agc-246592587427-ap-southeast-2/ComparativeDataSources/ncbi/fcs/gxdb/r2023-01-24" - }, - "busco": { - "mode": "geno", - "lineage_datasets": ["fungi_odb10", "hypocreales_odb10"], - "download_path": null - }, - "tidk": { - "repeat_seq": "TTAGGG" - }, - "lai": { - "monoploid_seqs": [ - [ - "FI1", - "https://raw.githubusercontent.com/Plant-Food-Research-Open/assembly_qc/main/docs/test_files/FI1.monoploid.seqs.txt" - ] - ] - }, - "kraken2": { - "db_path": "s3://agc-246592587427-ap-southeast-2/ComparativeDataSources/kraken2db/k2_pluspfp_20231009" - }, - "hic": { - "paired_reads": "SRR8238190" - }, - "synteny": { - "assembly_seq_list": [ - [ - "FI1", - "https://raw.githubusercontent.com/Plant-Food-Research-Open/assembly_qc/main/docs/test_files/FI1.seq.list" - ] - ], - "xref_assemblies": [ - [ - "TT_2021a", - "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/021/950/295/GCA_021950295.1_ASM2195029v1/GCA_021950295.1_ASM2195029v1_genomic.fna.gz", - "https://raw.githubusercontent.com/Plant-Food-Research-Open/assembly_qc/main/docs/test_files/TT_2021a.seq.list" - ] - ] - }, - "outdir": "s3://agc-246592587427-ap-southeast-2/output/assembly_qc/tests/test-full-results", - "max_cpus": 96, - "max_memory": "512.GB", - "max_time": "6.hours" -} diff --git a/docs/usage.md b/docs/usage.md new file mode 100644 index 00000000..1a98b70a --- /dev/null +++ b/docs/usage.md @@ -0,0 +1,222 @@ +# plant-food-research-open/assemblyqc: Usage + +> _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._ + +## Introduction + + + +## Samplesheet input + +You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row as shown in the examples below. + +```bash +--input '[path to samplesheet file]' +``` + +### Multiple runs of the same sample + +The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: + +```csv title="samplesheet.csv" +sample,fastq_1,fastq_2 +CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz +CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz +CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz,AEG588A1_S1_L004_R2_001.fastq.gz +``` + +### Full samplesheet + +The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 3 columns to match those defined in the table below. + +A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. + +```csv title="samplesheet.csv" +sample,fastq_1,fastq_2 +CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz +CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz +CONTROL_REP3,AEG588A3_S3_L002_R1_001.fastq.gz,AEG588A3_S3_L002_R2_001.fastq.gz +TREATMENT_REP1,AEG588A4_S4_L003_R1_001.fastq.gz, +TREATMENT_REP2,AEG588A5_S5_L003_R1_001.fastq.gz, +TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz, +TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz, +``` + +| Column | Description | +| --------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | +| `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). | +| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | + +An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. + +## Running the pipeline + +The typical command for running the pipeline is as follows: + +```bash +nextflow run plant-food-research-open/assemblyqc --input ./samplesheet.csv --outdir ./results --genome GRCh37 -profile docker +``` + +This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. + +Note that the pipeline will create the following files in your working directory: + +```bash +work # Directory containing the nextflow working files + # Finished results in specified location (defined with --outdir) +.nextflow_log # Log file from Nextflow +# Other nextflow hidden files, eg. history of pipeline runs and old logs. +``` + +If you wish to repeatedly use the same parameters for multiple runs, rather than specifying each flag in the command, you can specify these in a params file. + +Pipeline settings can be provided in a `yaml` or `json` file via `-params-file `. + +:::warning +Do not use `-c ` to specify parameters as this will result in errors. Custom config files specified with `-c` must only be used for [tuning process resource specifications](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources), other infrastructural tweaks (such as output directories), or module arguments (args). +::: + +The above pipeline run specified with a params file in yaml format: + +```bash +nextflow run plant-food-research-open/assemblyqc -profile docker -params-file params.yaml +``` + +with `params.yaml` containing: + +```yaml +input: './samplesheet.csv' +outdir: './results/' +genome: 'GRCh37' +<...> +``` + +You can also generate such `YAML`/`JSON` files via [nf-core/launch](https://nf-co.re/launch). + +### Updating the pipeline + +When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline: + +```bash +nextflow pull plant-food-research-open/assemblyqc +``` + +### Reproducibility + +It is a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since. + +First, go to the [plant-food-research-open/assemblyqc releases page](https://github.com/plant-food-research-open/assemblyqc/releases) and find the latest pipeline version - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. Of course, you can switch to another version by changing the number after the `-r` flag. + +This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. For example, at the bottom of the MultiQC reports. + +To further assist in reproducbility, you can use share and re-use [parameter files](#running-the-pipeline) to repeat pipeline runs with the same settings without having to write out a command with every single parameter. + +:::tip +If you wish to share such profile (such as upload as supplementary material for academic publications), make sure to NOT include cluster specific paths to files, nor institutional specific profiles. +::: + +## Core Nextflow arguments + +:::note +These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen). +::: + +### `-profile` + +Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments. + +Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Apptainer, Conda) - see below. + +:::info +We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. +::: + +The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to see if your system is available in these configs please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation). + +Note that multiple profiles can be loaded, for example: `-profile test,docker` - the order of arguments is important! +They are loaded in sequence, so later profiles can overwrite earlier profiles. + +If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended, since it can lead to different results on different machines dependent on the computer enviroment. + +- `test` + - A profile with a complete configuration for automated testing + - Includes links to test data so needs no other parameters +- `docker` + - A generic configuration profile to be used with [Docker](https://docker.com/) +- `singularity` + - A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/) +- `podman` + - A generic configuration profile to be used with [Podman](https://podman.io/) +- `shifter` + - A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/) +- `charliecloud` + - A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/) +- `apptainer` + - A generic configuration profile to be used with [Apptainer](https://apptainer.org/) +- `conda` + - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter, Charliecloud, or Apptainer. + +### `-resume` + +Specify this when restarting a pipeline. Nextflow will use cached results from any pipeline steps where the inputs are the same, continuing from where it got to previously. For input to be considered the same, not only the names must be identical but the files' contents as well. For more info about this parameter, see [this blog post](https://www.nextflow.io/blog/2019/demystifying-nextflow-resume.html). + +You can also supply a run name to resume a specific run: `-resume [run-name]`. Use the `nextflow log` command to show previous run names. + +### `-c` + +Specify the path to a specific config file (this is a core Nextflow command). See the [nf-core website documentation](https://nf-co.re/usage/configuration) for more information. + +## Custom configuration + +### Resource requests + +Whilst the default requirements set within the pipeline will hopefully work for most people and with most input data, you may find that you want to customise the compute resources that the pipeline requests. Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with any of the error codes specified [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L18) it will automatically be resubmitted with higher requests (2 x original, then 3 x original). If it still fails after the third attempt then the pipeline execution is stopped. + +To change the resource requests, please see the [max resources](https://nf-co.re/docs/usage/configuration#max-resources) and [tuning workflow resources](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources) section of the nf-core website. + +### Custom Containers + +In some cases you may wish to change which container or conda environment a step of the pipeline uses for a particular tool. By default nf-core pipelines use containers and software from the [biocontainers](https://biocontainers.pro/) or [bioconda](https://bioconda.github.io/) projects. However in some cases the pipeline specified version maybe out of date. + +To use a different container from the default container or conda environment specified in a pipeline, please see the [updating tool versions](https://nf-co.re/docs/usage/configuration#updating-tool-versions) section of the nf-core website. + +### Custom Tool Arguments + +A pipeline might not always support every possible argument or option of a particular tool used in pipeline. Fortunately, nf-core pipelines provide some freedom to users to insert additional parameters that the pipeline does not include by default. + +To learn how to provide additional arguments to a particular tool of the pipeline, please see the [customising tool arguments](https://nf-co.re/docs/usage/configuration#customising-tool-arguments) section of the nf-core website. + +### nf-core/configs + +In most cases, you will only need to create a custom config as a one-off but if you and others within your organisation are likely to be running nf-core pipelines regularly and need to use the same settings regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter. You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile. + +See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config.html) for more information about creating your own configuration files. + +If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack) on the [`#configs` channel](https://nfcore.slack.com/channels/configs). + +## Azure Resource Requests + +To be used with the `azurebatch` profile by specifying the `-profile azurebatch`. +We recommend providing a compute `params.vm_type` of `Standard_D16_v3` VMs by default but these options can be changed if required. + +Note that the choice of VM size depends on your quota and the overall workload during the analysis. +For a thorough list, please refer the [Azure Sizes for virtual machines in Azure](https://docs.microsoft.com/en-us/azure/virtual-machines/sizes). + +## Running in the background + +Nextflow handles job submissions and supervises the running jobs. The Nextflow process must run until the pipeline is finished. + +The Nextflow `-bg` flag launches Nextflow in the background, detached from your terminal so that the workflow does not stop if you log out of your session. The logs are saved to a file. + +Alternatively, you can use `screen` / `tmux` or similar tool to create a detached session which you can log back into at a later time. +Some HPC setups also allow you to run nextflow within a cluster job submitted your job scheduler (from where it submits more jobs). + +## Nextflow memory requirements + +In some cases, the Nextflow Java virtual machines can start to request a large amount of memory. +We recommend adding the following line to your environment to limit this (typically in `~/.bashrc` or `~./bash_profile`): + +```bash +NXF_OPTS='-Xms1g -Xmx4g' +``` diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy new file mode 100755 index 00000000..4ce590c0 --- /dev/null +++ b/lib/NfcoreTemplate.groovy @@ -0,0 +1,351 @@ +// +// This file holds several functions used within the nf-core pipeline template. +// + +import org.yaml.snakeyaml.Yaml +import groovy.json.JsonOutput +import nextflow.extension.FilesEx + +class NfcoreTemplate { + + // + // Check AWS Batch related parameters have been specified correctly + // + public static void awsBatch(workflow, params) { + if (workflow.profile.contains('awsbatch')) { + // Check params.awsqueue and params.awsregion have been set if running on AWSBatch + assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" + // Check outdir paths to be S3 buckets if running on AWSBatch + assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" + } + } + + // + // Warn if a -profile or Nextflow config has not been provided to run the pipeline + // + public static void checkConfigProvided(workflow, log) { + if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { + log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + + "Please refer to the quick start section and usage docs for the pipeline.\n " + } + } + + // + // Generate version string + // + public static String version(workflow) { + String version_string = "" + + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string + } + + // + // Construct and send completion email + // + public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = NfcoreTemplate.version(workflow) + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + + // Check if we are only sending emails on failure + def email_address = params.email + if (!params.email && params.email_on_fail && !workflow.success) { + email_address = params.email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("$projectDir/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("$projectDir/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("$projectDir/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(params.monochrome_logs) + if (email_address) { + try { + if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { + mail_cmd += [ '-A', mqc_report ] + } + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() + } + + // + // Construct and send a notification to a web server as JSON + // e.g. Microsoft Teams and Slack + // + public static void IM_notification(workflow, params, summary_params, projectDir, log) { + def hook_url = params.hook_url + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) misc_fields['repository'] = workflow.repository + if (workflow.commitId) misc_fields['commitid'] = workflow.commitId + if (workflow.revision) misc_fields['revision'] = workflow.revision + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + + def msg_fields = [:] + msg_fields['version'] = NfcoreTemplate.version(workflow) + msg_fields['runName'] = workflow.runName + msg_fields['success'] = workflow.success + msg_fields['dateComplete'] = workflow.complete + msg_fields['duration'] = workflow.duration + msg_fields['exitStatus'] = workflow.exitStatus + msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + msg_fields['errorReport'] = (workflow.errorReport ?: 'None') + msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") + msg_fields['projectDir'] = workflow.projectDir + msg_fields['summary'] = summary << misc_fields + + // Render the JSON template + def engine = new groovy.text.GStringTemplateEngine() + // Different JSON depending on the service provider + // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format + def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" + def hf = new File("$projectDir/assets/${json_path}") + def json_template = engine.createTemplate(hf).make(msg_fields) + def json_message = json_template.toString() + + // POST + def post = new URL(hook_url).openConnection(); + post.setRequestMethod("POST") + post.setDoOutput(true) + post.setRequestProperty("Content-Type", "application/json") + post.getOutputStream().write(json_message.getBytes("UTF-8")); + def postRC = post.getResponseCode(); + if (! postRC.equals(200)) { + log.warn(post.getErrorStream().getText()); + } + } + + // + // Dump pipeline parameters in a json file + // + public static void dump_parameters(workflow, params) { + def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = JsonOutput.toJson(params) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() + } + + // + // Print pipeline summary on completion + // + public static void summary(workflow, params, log) { + Map colors = logColours(params.monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } + } + + // + // ANSII Colours used for terminal logging + // + public static Map logColours(Boolean monochrome_logs) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes + } + + // + // Does what is says on the tin + // + public static String dashedLine(monochrome_logs) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" + } + + // + // nf-core logo + // + public static String logo(workflow, monochrome_logs) { + Map colors = logColours(monochrome_logs) + String workflow_version = NfcoreTemplate.version(workflow) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.purple} ${workflow.manifest.name} ${workflow_version}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) + } +} diff --git a/lib/Utils.groovy b/lib/Utils.groovy new file mode 100644 index 00000000..8d030f4e --- /dev/null +++ b/lib/Utils.groovy @@ -0,0 +1,47 @@ +// +// This file holds several Groovy functions that could be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml + +class Utils { + + // + // When running with -profile conda, warn if channels have not been set-up appropriately + // + public static void checkCondaChannels(log) { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + // This channel list is ordered by required channel priority. + def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean + + // Check that they are in the right order + def channel_priority_violation = false + def n = required_channels_in_order.size() + for (int i = 0; i < n - 1; i++) { + channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + } + + if (channels_missing | channel_priority_violation) { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/\n" + + " The observed channel order is \n" + + " ${channels}\n" + + " but the following channel order is required:\n" + + " ${required_channels_in_order}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + } + } +} diff --git a/lib/WorkflowAssemblyqc.groovy b/lib/WorkflowAssemblyqc.groovy new file mode 100755 index 00000000..8be44f94 --- /dev/null +++ b/lib/WorkflowAssemblyqc.groovy @@ -0,0 +1,122 @@ +// +// This file holds several functions specific to the workflow/assemblyqc.nf in the plant-food-research-open/assemblyqc pipeline +// + +import nextflow.Nextflow +import groovy.text.SimpleTemplateEngine + +class WorkflowAssemblyqc { + + // + // Check and validate parameters + // + public static void initialise(params, log) { + + genomeExistsError(params, log) + + + if (!params.fasta) { + Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." + } + } + + // + // Get workflow summary for MultiQC + // + public static String paramsSummaryMultiqc(workflow, summary) { + String summary_section = '' + for (group in summary.keySet()) { + def group_params = summary.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

$group

\n" + summary_section += " \n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + return yaml_file_text + } + + // + // Generate methods description for MultiQC + // + + public static String toolCitationText(params) { + + // TODO nf-core: Optionally add in-text citation tools to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def citation_text = [ + "Tools used in the workflow included:", + "FastQC (Andrews 2010),", + "MultiQC (Ewels et al. 2016)", + "." + ].join(' ').trim() + + return citation_text + } + + public static String toolBibliographyText(params) { + + // TODO Optionally add bibliographic entries to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def reference_text = [ + "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " + ].join(' ').trim() + + return reference_text + } + + public static String methodsDescriptionText(run_workflow, mqc_methods_yaml, params) { + // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + def meta = [:] + meta.workflow = run_workflow.toMap() + meta["manifest_map"] = run_workflow.manifest.toMap() + + // Pipeline DOI + meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" + meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + + // Tool references + meta["tool_citations"] = "" + meta["tool_bibliography"] = "" + + // TODO Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! + //meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + //meta["tool_bibliography"] = toolBibliographyText(params) + + + def methods_text = mqc_methods_yaml.text + + def engine = new SimpleTemplateEngine() + def description_html = engine.createTemplate(methods_text).make(meta) + + return description_html + } + + // + // Exit pipeline if incorrect --genome key provided + // + private static void genomeExistsError(params, log) { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + Nextflow.error(error_string) + } + } +} diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy new file mode 100755 index 00000000..b7f82557 --- /dev/null +++ b/lib/WorkflowMain.groovy @@ -0,0 +1,63 @@ +// +// This file holds several functions specific to the main.nf workflow in the plant-food-research-open/assemblyqc pipeline +// + +import nextflow.Nextflow + +class WorkflowMain { + + // + // Citation string for pipeline + // + public static String citation(workflow) { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + // TODO nf-core: Add Zenodo DOI for pipeline after first release + //"* The pipeline\n" + + //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" + } + + + // + // Validate parameters and print summary to screen + // + public static void initialise(workflow, params, log) { + + // Print workflow version and exit on --version + if (params.version) { + String workflow_version = NfcoreTemplate.version(workflow) + log.info "${workflow.manifest.name} ${workflow_version}" + System.exit(0) + } + + // Check that a -profile or Nextflow config has been provided to run the pipeline + NfcoreTemplate.checkConfigProvided(workflow, log) + + // Check that conda channels are set-up correctly + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + Utils.checkCondaChannels(log) + } + + // Check AWS batch settings + NfcoreTemplate.awsBatch(workflow, params) + + // Check input has been provided + if (!params.input) { + Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'") + } + } + // + // Get attribute from genome config file e.g. fasta + // + public static Object getGenomeAttribute(params, attribute) { + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + return params.genomes[ params.genome ][ attribute ] + } + } + return null + } +} diff --git a/lib/nfcore_external_java_deps.jar b/lib/nfcore_external_java_deps.jar new file mode 100644 index 0000000000000000000000000000000000000000..805c8bb5e4fd43a12a5891eea5a68788309629b0 GIT binary patch literal 2291171 zcma%i1CS<7mu}m(-Ea4_?dhK8v~AnAZQHhO+qP}n-E-&Lf8*Yb{bM(7MpZ;s){{|L znGpxiIY(9k6buar3JMCSNkdW|=)W{*ATS^)VMTsgF=-L{?{Od?P#{?eNT`1hApZ%I z{U0Zz{YCuW$x{5%Vj{u{igZ#U4^op8k`lCZbMO+hRMV3)4e|_&%)3YSbmCGpVp4NX zni_mKLGakt&P12{r^KCD0GPa9sIuy`fu+agt?8O-hY9i{WloGe}y@kSsDEY_Fud9 z-?2ab4J)MQXe4E3Xl-U<>PRMQZ)0m@@90KnV5#Te5Tz`pvZ0Lj4F<9%Flh(`Qb;Tn zMV!iFmH{S^fF*j#MCKRUd`7m>9||*;WE32I_6pg4-Q1C#ke$$aVmP-TtfJ+j+N9;Q 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+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Github : https://github.com/plant-food-research-open/assemblyqc +---------------------------------------------------------------------------------------- +*/ -nextflow.enable.dsl=2 +nextflow.enable.dsl = 2 -include { ASSEMBLYQC } from './workflows/assemblyqc.nf' +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + GENOME PARAMETER VALUES +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ -workflow { - PFR_ASSEMBLYQC() +// TODO nf-core: Remove this line if you don't need a FASTA file +// This is an example of how to use getGenomeAttribute() to fetch parameters +// from igenomes.config using `--genome` +params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + VALIDATE & PRINT PARAMETER SUMMARY +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +include { validateParameters; paramsHelp } from 'plugin/nf-validation' + +// Print help message if needed +if (params.help) { + def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) + def citation = '\n' + WorkflowMain.citation(workflow) + '\n' + def String command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker" + log.info logo + paramsHelp(command) + citation + NfcoreTemplate.dashedLine(params.monochrome_logs) + System.exit(0) +} + +// Validate input parameters +if (params.validate_params) { + validateParameters() +} + +WorkflowMain.initialise(workflow, params, log) + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + NAMED WORKFLOW FOR PIPELINE +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +include { ASSEMBLYQC } from './workflows/assemblyqc' + +// +// WORKFLOW: Run main plant-food-research-open/assemblyqc analysis pipeline +// +workflow PLANTFOODRESEARCHOPEN_ASSEMBLYQC { + ASSEMBLYQC () } -workflow PFR_ASSEMBLYQC { - ASSEMBLYQC() +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + RUN ALL WORKFLOWS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +// +// WORKFLOW: Execute a single named workflow for the pipeline +// See: https://github.com/nf-core/rnaseq/issues/619 +// +workflow { + PLANTFOODRESEARCHOPEN_ASSEMBLYQC () } + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + THE END +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ diff --git a/modules.json b/modules.json index 67f866b4..f3c7519d 100644 --- a/modules.json +++ b/modules.json @@ -1,175 +1,26 @@ { - "name": "", - "homePage": "", + "name": "plant-food-research-open/assemblyqc", + "homePage": "https://github.com/plant-food-research-open/assemblyqc", "repos": { - "git@github.com:PlantandFoodResearch/nxf-modules.git": { - "modules": { - "pfr": { - "bwa/index": { - "branch": "main", - "git_sha": "4b9da80b1e4c16067babd97554bea42d7cd9ca85", - "installed_by": ["fastq_bwa_mem_samblaster"] - }, - "bwa/mem": { - "branch": "main", - "git_sha": "4b9da80b1e4c16067babd97554bea42d7cd9ca85", - "installed_by": ["fastq_bwa_mem_samblaster"] - }, - "cat/cat": { - "branch": "main", - "git_sha": "a2c6aa54917a232ac543d88edc5d3c3d8bf71ef3", - "installed_by": ["fasta_ltrretriever_lai"] - }, - "custom/checkgff3fastacorrespondence": { - "branch": "main", - "git_sha": "1a76f884082c786760559c462063a5d1de94ca83", - "installed_by": ["gff3_validate"] - }, - "custom/restoregffids": { - "branch": "main", - "git_sha": "e9f6bdd634bdbcd52c5568ba82f16176ec06631f", - "installed_by": ["fasta_ltrretriever_lai"] - }, - "custom/shortenfastaids": { - "branch": "main", - "git_sha": "5e0e41b51d7fc7f68ae43692b6fe19b95d7f3a8c", - "installed_by": ["fasta_ltrretriever_lai"] - }, - "edta/ltrharvest": { - "branch": "main", - "git_sha": "5a3fa7e2643328a95ced3632b761edea710abe07", - "installed_by": ["fasta_ltrretriever_lai"] - }, - "gt/gff3": { - "branch": "main", - "git_sha": "bfa4874d3942bdff70cb8df17322834125cafb28", - "installed_by": ["gff3_validate"] - }, - "gt/gff3validator": { - "branch": "main", - "git_sha": "889b9b57b611dcb063594608c2a222c928327cba", - "installed_by": ["gff3_validate"] - }, - "gt/stat": { - "branch": "main", - "git_sha": "cb5fb0be78a98fd1e32b7c90d6adf8c3bf44133e", - "installed_by": ["modules"] - }, - "lai": { - "branch": "main", - "git_sha": "7e6e3cb41362a045c6bb6065903efa0eba246e87", - "installed_by": ["fasta_ltrretriever_lai"] - }, - "ltrfinder": { - "branch": "main", - "git_sha": "529f900f88ac36fded55f89e92b9dc2ee2c2b813", - "installed_by": ["fasta_ltrretriever_lai"] - }, - "ltrretriever": { - "branch": "main", - "git_sha": "47555fcab8a3871c618e779729e109b47b7b5974", - "installed_by": ["fasta_ltrretriever_lai"] - }, - "samblaster": { - "branch": "main", - "git_sha": "32ef0605651e46c3f5720ed55d21dc35ec371001", - "installed_by": ["fastq_bwa_mem_samblaster"] - } - } - }, - "subworkflows": { - "pfr": { - "fasta_ltrretriever_lai": { - "branch": "main", - "git_sha": "957519612296ca52e6c070883557a25beeabca55", - "installed_by": ["subworkflows"] - }, - "fastq_bwa_mem_samblaster": { - "branch": "main", - "git_sha": "9639ac9a556898d0f0e8592bff24585c33326458", - "installed_by": ["subworkflows"] - }, - "gff3_validate": { - "branch": "main", - "git_sha": "3fa824f807c433eebd154a424c2e50e1dae7b050", - "installed_by": ["subworkflows"] - } - } - } - }, "https://github.com/nf-core/modules.git": { "modules": { "nf-core": { "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "de45447d060b8c8b98575bc637a4a575fd0638e1", + "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e", "installed_by": ["modules"] }, - "fastavalidator": { - "branch": "master", - "git_sha": "89ff95427f695086369d7927a3c17cea2a37a382", - "installed_by": ["modules"] - }, - "fastp": { - "branch": "master", - "git_sha": "003920c7f9a8ae19b69a97171922880220bedf56", - "installed_by": ["fastq_trim_fastp_fastqc"] - }, "fastqc": { "branch": "master", - "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", - "installed_by": ["fastq_trim_fastp_fastqc"] - }, - "gunzip": { - "branch": "master", - "git_sha": "3a5fef109d113b4997c9822198664ca5f2716208", + "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", "installed_by": ["modules"] }, - "seqkit/seq": { - "branch": "master", - "git_sha": "687ad41c14008d3d55cf7c2ffacebe6a057211a4", - "installed_by": ["fasta_explore_search_plot_tidk"] - }, - "seqkit/sort": { - "branch": "master", - "git_sha": "ffcdfb354f8c8e59bdccc1a4249f0f3b232c2a3d", - "installed_by": ["fasta_explore_search_plot_tidk"] - }, - "tidk/explore": { - "branch": "master", - "git_sha": "fad335b31b32d78f89a8340373d3ccfc0f2b18b5", - "installed_by": ["fasta_explore_search_plot_tidk"] - }, - "tidk/plot": { - "branch": "master", - "git_sha": "d54cd1d823f151854825da8ffe7289bcbf77206b", - "installed_by": ["fasta_explore_search_plot_tidk"] - }, - "tidk/search": { + "multiqc": { "branch": "master", - "git_sha": "669a329f4aa37f5b7f03776c2ed1cd0ef122c626", - "installed_by": ["fasta_explore_search_plot_tidk"] - }, - "untar": { - "branch": "master", - "git_sha": "e719354ba77df0a1bd310836aa2039b45c29d620", + "git_sha": "4ab13872435962dadc239979554d13709e20bf29", "installed_by": ["modules"] } } - }, - "subworkflows": { - "nf-core": { - "fasta_explore_search_plot_tidk": { - "branch": "master", - "git_sha": "2b21fbeb20ad9f17612f4a3dd7b12971513f08d5", - "installed_by": ["subworkflows"] - }, - "fastq_trim_fastp_fastqc": { - "branch": "master", - "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": ["subworkflows"] - } - } } } } diff --git a/modules/local/agp2_assembly.nf b/modules/local/agp2_assembly.nf deleted file mode 100644 index b78450ac..00000000 --- a/modules/local/agp2_assembly.nf +++ /dev/null @@ -1,19 +0,0 @@ -process AGP2_ASSEMBLY { - tag "$sample_id_on_tag" - label "process_single" - - container "docker.io/gallvp/juicebox_scripts:a7ae991_ps" - publishDir "${params.outdir}/hic/assembly", mode:'copy' - - input: - tuple val(sample_id_on_tag), path(agp_file) - - output: - tuple val(sample_id_on_tag), path("*.agp.assembly"), emit: assembly - - script: - """ - assembly_tag=\$(echo $sample_id_on_tag | sed 's/.*\\.on\\.//g') - agp2assembly.py $agp_file "\${assembly_tag}.agp.assembly" - """ -} diff --git a/modules/local/assemblathon_stats.nf b/modules/local/assemblathon_stats.nf deleted file mode 100644 index edd85107..00000000 --- a/modules/local/assemblathon_stats.nf +++ /dev/null @@ -1,43 +0,0 @@ -process ASSEMBLATHON_STATS { - tag "${hap_name}" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/ubuntu:20.04': - 'quay.io/nf-core/ubuntu:20.04' }" - - input: - tuple val(hap_name), path(fasta_file) - - output: - path "${hap_name}_stats.csv" , emit: stats - path 'versions.yml' , emit: versions - - script: - def VERSION = "github/PlantandFoodResearch/assemblathon2-analysis/a93cba2" - """ - paths_to_check=\$(printf "%s\\n" \$(echo \$PATH | tr ':' ' ') \\ - | xargs -I {} find {} -maxdepth 0 -print 2>/dev/null \\ - | grep -v '^\$' \\ - | grep -v '/sbin' \\ - | xargs - ) - - falite_path="\$(find \$paths_to_check -name FAlite_943e0fb.pm)" - - ln -s "\$falite_path" FAlite_943e0fb.pm - - PERL5LIB=./ assemblathon_stats_943e0fb.pl \\ - -n ${params.assemblathon_stats.n_limit} \\ - -csv \\ - "${fasta_file}" - - csv_file_name=\$(ls | grep "csv") - mv \$csv_file_name "${hap_name}_stats.csv" - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - assemblathon_stats: $VERSION - END_VERSIONS - """ -} diff --git a/modules/local/assembly2_bedpe.nf b/modules/local/assembly2_bedpe.nf deleted file mode 100644 index 9d117e50..00000000 --- a/modules/local/assembly2_bedpe.nf +++ /dev/null @@ -1,19 +0,0 @@ -process ASSEMBLY2_BEDPE { - tag "$sample_id_on_tag" - label "process_single" - - container "docker.io/gallvp/python3npkgs:v0.4" - publishDir "${params.outdir}/hic/bedpe", mode:'copy' - - input: - tuple val(sample_id_on_tag), path(agp_assembly_file) - - output: - tuple val(sample_id_on_tag), path("*.assembly.bedpe"), emit: agp_assembly_bedpe_file - - script: - """ - assembly_tag=\$(echo $sample_id_on_tag | sed 's/.*\\.on\\.//g') - assembly_2_bedpe_943e0fb.py $agp_assembly_file > "\${assembly_tag}.assembly.bedpe" - """ -} diff --git a/modules/local/create_report.nf b/modules/local/create_report.nf deleted file mode 100644 index 6a9fd169..00000000 --- a/modules/local/create_report.nf +++ /dev/null @@ -1,33 +0,0 @@ -process CREATE_REPORT { - tag "AssemblyQC" - label "process_single" - - // container "docker.io/gallvp/python3npkgs:v0.4" - publishDir params.outdir, mode: 'copy' - - input: - path fastavalidator_logs, stageAs: 'fastavalidator_logs/*' - path gff3_validate_logs, stageAs: 'gff3_validate_logs/*' - path ncbi_fcs_adaptor_reports, stageAs: 'ncbi_fcs_adaptor_reports/*' - path fcs_gx_reports, stageAs: 'fcs_gx_reports/*' - path assemblathon_stats, stageAs: 'assemblathon_stats/*' - path genometools_gt_stats, stageAs: 'genometools_gt_stat/*' - path busco_outputs, stageAs: 'busco_outputs/*' - path tidk_plots, stageAs: 'tidk_outputs/*' - path lai_outputs, stageAs: 'lai_outputs/*' - path kraken2_outputs, stageAs: 'kraken2_outputs/*' - path hic_outputs, stageAs: 'hic_outputs/*' - path circos_outputs, stageAs: 'circos_outputs/*' - path versions - val params_json - - output: - path 'report.html' - path 'report.json' - - script: - """ - echo -n '$params_json' > params_json.json - assembly_qc_report_943e0fb.py > report.html - """ -} diff --git a/modules/local/hic_qc.nf b/modules/local/hic_qc.nf deleted file mode 100644 index cc87c68c..00000000 --- a/modules/local/hic_qc.nf +++ /dev/null @@ -1,21 +0,0 @@ -process HIC_QC { - tag "$sample_id_on_tag" - label "process_single" - - publishDir "${params.outdir}/hic/hic_qc", mode:'copy' - container "docker.io/gallvp/hic_qc:6881c33_ps" - - input: - tuple val(sample_id_on_tag), path(dedup_bam) - - output: - tuple val(sample_id_on_tag), path("*.pdf") - - script: - """ - hic_qc.py \ - -n 10000000 \ - -b "${dedup_bam}" \ - --outfile_prefix "$sample_id_on_tag" - """ -} diff --git a/modules/local/juicer_sort.nf b/modules/local/juicer_sort.nf deleted file mode 100644 index c9b0577f..00000000 --- a/modules/local/juicer_sort.nf +++ /dev/null @@ -1,22 +0,0 @@ -process JUICER_SORT { - tag "$sample_id_on_tag" - label "process_high" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/ubuntu:20.04': - 'quay.io/nf-core/ubuntu:20.04' }" - - input: - tuple val(sample_id_on_tag), path(out_links_txt) - - output: - tuple val(sample_id_on_tag), path("*sorted.links.txt"), emit: links - - script: - """ - sort --parallel=${task.cpus} \\ - -k2,2 -k6,6 \\ - $out_links_txt \\ - > out.sorted.links.txt - """ -} diff --git a/modules/local/make_agp_from_fasta.nf b/modules/local/make_agp_from_fasta.nf deleted file mode 100644 index 6541b3e5..00000000 --- a/modules/local/make_agp_from_fasta.nf +++ /dev/null @@ -1,18 +0,0 @@ -process MAKE_AGP_FROM_FASTA { - tag "$sample_id_on_tag" - label "process_single" - - container "docker.io/gallvp/juicebox_scripts:a7ae991_ps" - - input: - tuple val(sample_id_on_tag), path(assembly_fasta) - - output: - tuple val(sample_id_on_tag), path("*.agp"), emit: agp_file - - script: - """ - file_name="$assembly_fasta" - makeAgpFromFasta.py $assembly_fasta "\${file_name%%.*}.agp" - """ -} diff --git a/modules/local/matlock_bam2_juicer.nf b/modules/local/matlock_bam2_juicer.nf deleted file mode 100644 index 4432f0c9..00000000 --- a/modules/local/matlock_bam2_juicer.nf +++ /dev/null @@ -1,19 +0,0 @@ -process MATLOCK_BAM2_JUICER { - tag "$sample_id_on_tag" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/matlock:20181227--h4b03ef3_3': - 'quay.io/biocontainers/matlock:20181227--h4b03ef3_3' }" - - input: - tuple val(sample_id_on_tag), path(hic_bam_scaffolds) - - output: - tuple val(sample_id_on_tag), path("out.links.txt") - - script: - """ - matlock bam2 juicer $hic_bam_scaffolds out.links.txt - """ -} diff --git a/modules/local/ncbi_fcs_adaptor.nf b/modules/local/ncbi_fcs_adaptor.nf deleted file mode 100644 index 9e14c0c5..00000000 --- a/modules/local/ncbi_fcs_adaptor.nf +++ /dev/null @@ -1,34 +0,0 @@ -process NCBI_FCS_ADAPTOR { - tag "${hap_name}" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://ftp.ncbi.nlm.nih.gov/genomes/TOOLS/FCS/releases/0.4.0/fcs-adaptor.sif': - 'docker.io/ncbi/fcs-adaptor:0.4.0' }" - - input: - tuple val(hap_name), path(fasta_file) - - output: - tuple val(hap_name), path("${hap_name}_fcs_adaptor_report.tsv") , emit: report - path "versions.yml" , emit: versions - - script: - def VERSION = 0.4 - """ - mkdir "${hap_name}_outputdir" - - /app/fcs/bin/av_screen_x \\ - -o "${hap_name}_outputdir" \\ - --${params.ncbi_fcs_adaptor.empire} \\ - "${fasta_file}" - - mv "${hap_name}_outputdir/fcs_adaptor_report.txt" \\ - "./${hap_name}_fcs_adaptor_report.tsv" - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - av_screen_x: $VERSION - END_VERSIONS - """ -} diff --git a/modules/local/run_assembly_visualizer.nf b/modules/local/run_assembly_visualizer.nf deleted file mode 100644 index 3f44a168..00000000 --- a/modules/local/run_assembly_visualizer.nf +++ /dev/null @@ -1,25 +0,0 @@ -process RUN_ASSEMBLY_VISUALIZER { - tag "$sample_id_on_tag" - label "process_medium" - - publishDir "${params.outdir}/hic", mode:'copy' - container "gallvp/3d-dna:63029aa" - - input: - tuple val(sample_id_on_tag), path(agp_assembly_file), path(sorted_links_txt_file) - - output: - tuple val(sample_id_on_tag), path("*.hic"), emit: hic - - script: - // -p true/false Use GNU Parallel to speed up computation (default is true). - """ - assembly_tag=\$(echo $sample_id_on_tag | sed 's/.*\\.on\\.//g') - file_name="${agp_assembly_file}" - - /usr/src/3d-dna/visualize/run-assembly-visualizer.sh \\ - $agp_assembly_file $sorted_links_txt_file - - mv "\${file_name%.*}.hic" "\${assembly_tag}.hic" - """ -} diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf new file mode 100644 index 00000000..7a776dbd --- /dev/null +++ b/modules/local/samplesheet_check.nf @@ -0,0 +1,31 @@ +process SAMPLESHEET_CHECK { + tag "$samplesheet" + label 'process_single' + + conda "conda-forge::python=3.8.3" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/python:3.8.3' : + 'biocontainers/python:3.8.3' }" + + input: + path samplesheet + + output: + path '*.csv' , emit: csv + path "versions.yml", emit: versions + + when: + task.ext.when == null || task.ext.when + + script: // This script is bundled with the pipeline, in plant-food-research-open/assemblyqc/bin/ + """ + check_samplesheet.py \\ + $samplesheet \\ + samplesheet.valid.csv + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + python: \$(python --version | sed 's/Python //g') + END_VERSIONS + """ +} diff --git a/modules/local/utils.nf b/modules/local/utils.nf deleted file mode 100644 index c3d3d8ea..00000000 --- a/modules/local/utils.nf +++ /dev/null @@ -1,228 +0,0 @@ -import groovy.json.JsonOutput - -def jsonifyParams(params) { - return JsonOutput.toJson(params) -} - -def validateParams(params) { - validateFastaTags(params) - validateGff3Tags(params) - validateGff3FastaCorrespondence(params) - - validateBUSCOParameters(params) - validateLAIParameters(params) - validateSyntenyParameters(params) -} - -def validateFastaTags(params) { - def listOfFastaTuples = params["target_assemblies"] - - if (isNotListOfLists(listOfFastaTuples, 2)) { - error 'Error: target_assemblies must be a list of sublists, with each sublist containing 2 elements' - } - - def fastaTags = listOfFastaTuples.collect { it[0] } - - fastaTags.each { - if (!(it =~ /^\w+$/)) { - error "Error: $it is not a valid tag in target_assemblies" - } - } - - if (fastaTags.size() != (fastaTags as Set).size()) { - error "All the tags in target_assemblies should be unique" - } -} - -def validateGff3Tags(params) { - def listOfGff3Tuples = params["assembly_gff3"] - - if (listOfGff3Tuples.isEmpty()) { - return - } - - if (isNotListOfLists(listOfGff3Tuples, 2)) { - error 'Error: assembly_gff3 must be a list of sublists, with each sublist containing 2 elements' - } - - def gff3Tags = listOfGff3Tuples.collect { it[0] } - - gff3Tags.each { - if (!(it =~ /^\w+$/)) { - error "Error: $it is not a valid tag in assembly_gff3" - } - } -} - -def validateGff3FastaCorrespondence(params) { - - def listOfGff3Tuples = params["assembly_gff3"] - def listOfFastaTuples = params["target_assemblies"] - - def fastaTags = listOfFastaTuples.collect { it[0] } - def gff3Tags = listOfGff3Tuples.collect { it[0] } - - gff3Tags.each { - if(!fastaTags.contains(it)) { - error "Error: $it in assembly_gff3 does not have a corresponding tag in target_assemblies" - } - } -} - -def validateBUSCOParameters(params) { - - if (params["busco"]["skip"] == 1) { - return - } - - listOfBUSCOLineages = params["busco"]["lineage_datasets"] - - if (!(listOfBUSCOLineages instanceof List)) { - error 'Error: busco::lineage_datasets must be a list of lineage(s)' - } -} - -def validateLAIParameters(params) { - - if (params["lai"]["skip"] == 1) { - return - } - - validateLAIMonoploidSeqs(params) -} - -def validateLAIMonoploidSeqs(params) { - - def listOfMonoploidSeqs = params["lai"]["monoploid_seqs"] - def listOfFastaTuples = params["target_assemblies"] - - if (listOfMonoploidSeqs.isEmpty()) { - return - } - - if (isNotListOfLists(listOfMonoploidSeqs, 2)) { - error 'Error: lai::monoploid_seqs must be a list of sublists, with each sublist containing 2 elements' - } - - def fastaTags = listOfFastaTuples.collect { it[0] } - def monoSeqTags = listOfFastaTuples.collect { it[0] } - - monoSeqTags.each { - if(!fastaTags.contains(it)) { - error "Error: $it in lai::monoploid_seqs does not have a corresponding tag in target_assemblies" - } - } - - listOfMonoploidSeqs.each { - validateMonoSeqs(it[1]) - } -} - -def validateSyntenyParameters(params) { - if (params["synteny"]["skip"] == 1) { - return - } - - def listOfFastaTuples = params["target_assemblies"] - def listOfTargetSeqLists = params["synteny"]["assembly_seq_list"] - - if (isNotListOfLists(listOfTargetSeqLists, 2)) { - error 'Error: synteny::assembly_seq_list must be a list of sublists, with each sublist containing 2 elements' - } - - if (listOfTargetSeqLists.size() != listOfFastaTuples.size()) { - error "Error: The number of elements in synteny::assembly_seq_list and target_assemblies should be equal" - } - - def fastaTags = listOfFastaTuples.collect { it[0] } - def seqListTags = listOfTargetSeqLists.collect { it[0] } - - if (!seqListTags.containsAll(fastaTags)) { - error "Error: The tags in synteny::assembly_seq_list should match the tags in target_assemblies" - } - - listOfTargetSeqLists.each { - validateSeqList(it[1]) - } - - def listOfXRefAssemblies = params["synteny"]["xref_assemblies"] - - if (listOfXRefAssemblies.isEmpty()) { - return - } - - if (isNotListOfLists(listOfXRefAssemblies, 3)) { - error 'Error: synteny::xref_assemblies must be a list of sublists, with each sublist containing 3 elements' - } - - def xrefTags = listOfXRefAssemblies.collect { it[0] } - - xrefTags.each { - if (!(it =~ /^\w+$/)) { - error "Error: $it is not a valid tag in synteny::xref_assemblies" - } - } - - if (xrefTags.size() != (xrefTags as Set).size()) { - error "All the tags in synteny::xref_assemblies should be unique" - } - - xrefTags.each { - if (fastaTags.contains(it)) { - error "Error: Tag $it in synteny::xref_assemblies is already included in target_assemblies" - } - } - - listOfXRefAssemblies.each { - validateSeqList(it[2]) - } -} - -def isNotListOfLists(thisOne, subListSize) { - return (!(thisOne instanceof List) || thisOne.isEmpty() || thisOne.any { !(it instanceof List) || it.size() != subListSize }) -} - -def validateSeqList(seqListPath) { - def seqListFile = file(seqListPath, checkIfExists: true) - - def lines = seqListFile.readLines() - if (lines.isEmpty()) { - error "${seqListPath} is empty. It should be a 2 column tab-delimited file" - } - - lines.each { line -> - def columns = line.split("\t") - if (columns.size() != 2) { - error "${seqListPath} should be a 2 column tab-delimited file" - } - } - - def column1Set = new HashSet<>() - def column2Set = new HashSet<>() - def hasUniqueElements = lines.every { line -> - def columns = line.split("\t") - def element1 = columns[0] - def element2 = columns[1] - column1Set.add(element1) && column2Set.add(element2) - } - - if (!hasUniqueElements) { - error "${seqListPath} contains duplicate elements in one or both columns" - } -} - -def validateMonoSeqs(monoSeqsPath) { - def monoSeqsFile = file(monoSeqsPath, checkIfExists: true) - - def lines = monoSeqsFile.readLines() - if (lines.isEmpty()) { - error "${monoSeqsPath} is empty. It should be a single column text file" - } - - lines.each { line -> - def literals = line.split() - if (literals.size() != 1) { - error "${monoSeqsPath} should be a single column text file" - } - } -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml index b48ced26..f0c63f69 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.20 + - bioconda::multiqc=1.17 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index 27f7f11b..7685b33c 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -3,9 +3,9 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' : - 'quay.io/biocontainers/multiqc:1.20--pyhdfd78af_0' }" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : + 'biocontainers/multiqc:1.17--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py index 4a993608..e55b8d43 100755 --- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py +++ b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py @@ -4,10 +4,11 @@ """Provide functions to merge multiple versions.yml files.""" -import yaml import platform from textwrap import dedent +import yaml + def _make_versions_html(versions): """Generate a tabular HTML output of all versions for MultiQC.""" @@ -58,9 +59,7 @@ def main(): } with open("$versions") as f: - versions_by_process = ( - yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module - ) + versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module # aggregate versions by the module name (derived from fully-qualified process name) versions_by_module = {} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test index 2455d302..eec1db10 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test @@ -31,13 +31,8 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot( - process.out.versions, - file(process.out.mqc_yml[0]).readLines()[0..10], - file(process.out.yml[0]).readLines()[0..7] - ).match() - } + { assert snapshot(process.out).match() } ) } } -} \ No newline at end of file +} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap index 5f59a936..4274ed57 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap @@ -1,33 +1,27 @@ { "Should run without failures": { "content": [ - [ - "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" - ], - [ - "data: \"\\n\\n \\n \\n \\n \\n \\n \\n \\n\\", - " \\n\\n\\n \\n \\n\\", - " \\ \\n\\n\\n\\n \\n \\", - " \\ \\n \\n\\n\\n\\n\\", - " \\n\\n \\n \\n\\", - " \\ \\n\\n\\n\\n\\n\\n \\n\\", - " \\ \\n \\n\\n\\n\\n\\", - " \\n\\n \\n \\n\\" - ], - [ - "CUSTOM_DUMPSOFTWAREVERSIONS:", - " python: 3.11.7", - " yaml: 5.4.1", - "TOOL1:", - " tool1: 0.11.9", - "TOOL2:", - " tool2: '1.9'", - "Workflow:" - ] + { + "0": [ + "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" + ], + "1": [ + "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" + ], + "2": [ + "versions.yml:md5,3843ac526e762117eedf8825b40683df" + ], + "mqc_yml": [ + "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" + ], + "versions": [ + "versions.yml:md5,3843ac526e762117eedf8825b40683df" + ], + "yml": [ + "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" + ] + } ], - "timestamp": "2024-01-09T23:01:18.710682" + "timestamp": "2023-11-03T14:43:22.157011" } -} \ No newline at end of file +} diff --git a/modules/nf-core/fastavalidator/environment.yml b/modules/nf-core/fastavalidator/environment.yml deleted file mode 100644 index 70f346ef..00000000 --- a/modules/nf-core/fastavalidator/environment.yml +++ /dev/null @@ -1,9 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json -name: "fastavalidator" -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - "bioconda::py_fasta_validator=0.6" diff --git a/modules/nf-core/fastavalidator/main.nf b/modules/nf-core/fastavalidator/main.nf deleted file mode 100644 index 8371dd6f..00000000 --- a/modules/nf-core/fastavalidator/main.nf +++ /dev/null @@ -1,62 +0,0 @@ -process FASTAVALIDATOR { - tag "$meta.id" - label 'process_single' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/py_fasta_validator:0.6--py37h595c7a6_0': - 'quay.io/biocontainers/py_fasta_validator:0.6--py37h595c7a6_0' }" - - input: - tuple val(meta), path(fasta) - - output: - tuple val(meta), path('*.success.log') , emit: success_log , optional: true - tuple val(meta), path('*.error.log') , emit: error_log , optional: true - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - py_fasta_validator \\ - -f $fasta \\ - 2> "${prefix}.error.log" \\ - || echo "Errors from fasta_validate printed to ${prefix}.error.log" - - if [ \$(cat "${prefix}.error.log" | wc -l) -gt 0 ]; then - echo "Validation failed..." - - cat \\ - "${prefix}.error.log" - else - echo "Validation successful..." - - mv \\ - "${prefix}.error.log" \\ - fasta_validate.stderr - - echo "Validation successful..." \\ - > "${prefix}.success.log" - fi - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - py_fasta_validator: \$(py_fasta_validator -v | sed 's/.* version //') - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - echo "Validation successful..." \\ - > "${prefix}.success.log" - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - py_fasta_validator: \$(py_fasta_validator -v | sed 's/.* version //') - END_VERSIONS - """ -} diff --git a/modules/nf-core/fastavalidator/meta.yml b/modules/nf-core/fastavalidator/meta.yml deleted file mode 100644 index c5c4371c..00000000 --- a/modules/nf-core/fastavalidator/meta.yml +++ /dev/null @@ -1,53 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: "fastavalidator" -description: | - "Python C-extension for a simple validator for fasta files. The module emits the validated file or an - error log upon validation failure." -keywords: - - fasta - - validation - - genome -tools: - - fasta_validate: - description: | - "Python C-extension for a simple C code to validate a fasta file. It only checks a few things, - and by default only sets its response via the return code, - so you will need to check that!" - homepage: "https://github.com/linsalrob/py_fasta_validator" - documentation: "https://github.com/linsalrob/py_fasta_validator" - tool_dev_url: "https://github.com/linsalrob/py_fasta_validator" - doi: "10.5281/zenodo.5002710" - licence: ["MIT"] -input: - - meta: - type: map - description: | - Groovy Map containing file information - e.g. [ id:'test' ] - - fasta: - type: file - description: Input fasta file - pattern: "*.fasta" -output: - - meta: - type: map - description: | - Groovy Map containing file information - e.g. [ id:'test' ] - - success_log: - type: file - description: Log file for successful validation - pattern: "*.success.log" - - error_log: - type: file - description: Log file for failed validation - pattern: "*.error.log" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@gallvp" -maintainers: - - "@gallvp" diff --git a/modules/nf-core/fastavalidator/tests/main.nf.test b/modules/nf-core/fastavalidator/tests/main.nf.test deleted file mode 100644 index 52766f49..00000000 --- a/modules/nf-core/fastavalidator/tests/main.nf.test +++ /dev/null @@ -1,60 +0,0 @@ -nextflow_process { - - name "Test Process FASTAVALIDATOR" - script "../main.nf" - process "FASTAVALIDATOR" - - tag "modules" - tag "modules_nfcore" - tag "fastavalidator" - - test("sarscov2-fasta-valid") { - - when { - process { - """ - input[0] = [ - [ id:'test', single_end:false ], // meta map - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert process.out.success_log != null }, - { assert process.out.error_log == [] }, - { assert path(process.out.success_log.get(0).get(1)).getText().contains("Validation successful...") } - ) - } - - } - - test("sarscov2-gff3-invalid") { - - when { - process { - """ - input[0] = [ - [ id:'test', single_end:false ], // meta map - file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert process.out.success_log == [] }, - { assert process.out.error_log != null }, - { assert path(process.out.error_log.get(0).get(1)).getText().contains("genome.gff3 does not start with a >") } - ) - } - - } -} \ No newline at end of file diff --git a/modules/nf-core/fastavalidator/tests/main.nf.test.snap b/modules/nf-core/fastavalidator/tests/main.nf.test.snap deleted file mode 100644 index 382dee72..00000000 --- a/modules/nf-core/fastavalidator/tests/main.nf.test.snap +++ /dev/null @@ -1,76 +0,0 @@ -{ - "sarscov2-fasta-valid": { - "content": [ - { - "0": [ - [ - { - "id": "test", - "single_end": false - }, - "test.success.log:md5,b0b859eda1db5cd43915846e00ebc22c" - ] - ], - "1": [ - - ], - "2": [ - "versions.yml:md5,05aa059840b3b4dd6d88bc1e4bf976d7" - ], - "error_log": [ - - ], - "success_log": [ - [ - { - "id": "test", - "single_end": false - }, - "test.success.log:md5,b0b859eda1db5cd43915846e00ebc22c" - ] - ], - "versions": [ - "versions.yml:md5,05aa059840b3b4dd6d88bc1e4bf976d7" - ] - } - ], - "timestamp": "2023-11-28T11:23:25.106872" - }, - "sarscov2-gff3-invalid": { - "content": [ - { - "0": [ - - ], - "1": [ - [ - { - "id": "test", - "single_end": false - }, - "test.error.log:md5,531d520c0e7767176f743f197f1f87b3" - ] - ], - "2": [ - "versions.yml:md5,05aa059840b3b4dd6d88bc1e4bf976d7" - ], - "error_log": [ - [ - { - "id": "test", - "single_end": false - }, - "test.error.log:md5,531d520c0e7767176f743f197f1f87b3" - ] - ], - "success_log": [ - - ], - "versions": [ - "versions.yml:md5,05aa059840b3b4dd6d88bc1e4bf976d7" - ] - } - ], - "timestamp": "2023-11-28T11:23:29.40324" - } -} \ No newline at end of file diff --git a/modules/nf-core/fastavalidator/tests/tags.yml b/modules/nf-core/fastavalidator/tests/tags.yml deleted file mode 100644 index c3c77576..00000000 --- a/modules/nf-core/fastavalidator/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -fastavalidator: - - "modules/nf-core/fastavalidator/**" diff --git a/modules/nf-core/fastp/main.nf b/modules/nf-core/fastp/main.nf deleted file mode 100644 index 96a89f62..00000000 --- a/modules/nf-core/fastp/main.nf +++ /dev/null @@ -1,120 +0,0 @@ -process FASTP { - tag "$meta.id" - label 'process_medium' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/fastp:0.23.4--h5f740d0_0' : - 'quay.io/biocontainers/fastp:0.23.4--h5f740d0_0' }" - - input: - tuple val(meta), path(reads) - path adapter_fasta - val save_trimmed_fail - val save_merged - - output: - tuple val(meta), path('*.fastp.fastq.gz') , optional:true, emit: reads - tuple val(meta), path('*.json') , emit: json - tuple val(meta), path('*.html') , emit: html - tuple val(meta), path('*.log') , emit: log - path "versions.yml" , emit: versions - tuple val(meta), path('*.fail.fastq.gz') , optional:true, emit: reads_fail - tuple val(meta), path('*.merged.fastq.gz'), optional:true, emit: reads_merged - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def adapter_list = adapter_fasta ? "--adapter_fasta ${adapter_fasta}" : "" - def fail_fastq = save_trimmed_fail && meta.single_end ? "--failed_out ${prefix}.fail.fastq.gz" : save_trimmed_fail && !meta.single_end ? "--unpaired1 ${prefix}_1.fail.fastq.gz --unpaired2 ${prefix}_2.fail.fastq.gz" : '' - // Added soft-links to original fastqs for consistent naming in MultiQC - // Use single ended for interleaved. Add --interleaved_in in config. - if ( task.ext.args?.contains('--interleaved_in') ) { - """ - [ ! -f ${prefix}.fastq.gz ] && ln -sf $reads ${prefix}.fastq.gz - - fastp \\ - --stdout \\ - --in1 ${prefix}.fastq.gz \\ - --thread $task.cpus \\ - --json ${prefix}.fastp.json \\ - --html ${prefix}.fastp.html \\ - $adapter_list \\ - $fail_fastq \\ - $args \\ - 2> >(tee ${prefix}.fastp.log >&2) \\ - | gzip -c > ${prefix}.fastp.fastq.gz - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g") - END_VERSIONS - """ - } else if (meta.single_end) { - """ - [ ! -f ${prefix}.fastq.gz ] && ln -sf $reads ${prefix}.fastq.gz - - fastp \\ - --in1 ${prefix}.fastq.gz \\ - --out1 ${prefix}.fastp.fastq.gz \\ - --thread $task.cpus \\ - --json ${prefix}.fastp.json \\ - --html ${prefix}.fastp.html \\ - $adapter_list \\ - $fail_fastq \\ - $args \\ - 2> >(tee ${prefix}.fastp.log >&2) - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g") - END_VERSIONS - """ - } else { - def merge_fastq = save_merged ? "-m --merged_out ${prefix}.merged.fastq.gz" : '' - """ - [ ! -f ${prefix}_1.fastq.gz ] && ln -sf ${reads[0]} ${prefix}_1.fastq.gz - [ ! -f ${prefix}_2.fastq.gz ] && ln -sf ${reads[1]} ${prefix}_2.fastq.gz - fastp \\ - --in1 ${prefix}_1.fastq.gz \\ - --in2 ${prefix}_2.fastq.gz \\ - --out1 ${prefix}_1.fastp.fastq.gz \\ - --out2 ${prefix}_2.fastp.fastq.gz \\ - --json ${prefix}.fastp.json \\ - --html ${prefix}.fastp.html \\ - $adapter_list \\ - $fail_fastq \\ - $merge_fastq \\ - --thread $task.cpus \\ - --detect_adapter_for_pe \\ - $args \\ - 2> >(tee ${prefix}.fastp.log >&2) - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g") - END_VERSIONS - """ - } - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - def is_single_output = task.ext.args?.contains('--interleaved_in') || meta.single_end - def touch_reads = is_single_output ? "${prefix}.fastp.fastq.gz" : "${prefix}_1.fastp.fastq.gz ${prefix}_2.fastp.fastq.gz" - def touch_merged = (!is_single_output && save_merged) ? "touch ${prefix}.merged.fastq.gz" : "" - """ - touch $touch_reads - touch "${prefix}.fastp.json" - touch "${prefix}.fastp.html" - touch "${prefix}.fastp.log" - $touch_merged - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g") - END_VERSIONS - """ -} diff --git a/modules/nf-core/fastp/meta.yml b/modules/nf-core/fastp/meta.yml deleted file mode 100644 index c22a16ab..00000000 --- a/modules/nf-core/fastp/meta.yml +++ /dev/null @@ -1,75 +0,0 @@ -name: fastp -description: Perform adapter/quality trimming on sequencing reads -keywords: - - trimming - - quality control - - fastq -tools: - - fastp: - description: | - A tool designed to provide fast all-in-one preprocessing for FastQ files. This tool is developed in C++ with multithreading supported to afford high performance. - documentation: https://github.com/OpenGene/fastp - doi: 10.1093/bioinformatics/bty560 - licence: ["MIT"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information. Use 'single_end: true' to specify single ended or interleaved FASTQs. Use 'single_end: false' for paired-end reads. - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: | - List of input FastQ files of size 1 and 2 for single-end and paired-end data, - respectively. If you wish to run interleaved paired-end data, supply as single-end data - but with `--interleaved_in` in your `modules.conf`'s `ext.args` for the module. - - adapter_fasta: - type: file - description: File in FASTA format containing possible adapters to remove. - pattern: "*.{fasta,fna,fas,fa}" - - save_trimmed_fail: - type: boolean - description: Specify true to save files that failed to pass trimming thresholds ending in `*.fail.fastq.gz` - - save_merged: - type: boolean - description: Specify true to save all merged reads to the a file ending in `*.merged.fastq.gz` -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: The trimmed/modified/unmerged fastq reads - pattern: "*fastp.fastq.gz" - - json: - type: file - description: Results in JSON format - pattern: "*.json" - - html: - type: file - description: Results in HTML format - pattern: "*.html" - - log: - type: file - description: fastq log file - pattern: "*.log" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - - reads_fail: - type: file - description: Reads the failed the preprocessing - pattern: "*fail.fastq.gz" - - reads_merged: - type: file - description: Reads that were successfully merged - pattern: "*.{merged.fastq.gz}" -authors: - - "@drpatelh" - - "@kevinmenden" -maintainers: - - "@drpatelh" - - "@kevinmenden" diff --git a/modules/nf-core/fastp/tests/main.nf.test b/modules/nf-core/fastp/tests/main.nf.test deleted file mode 100644 index 7ccd9b59..00000000 --- a/modules/nf-core/fastp/tests/main.nf.test +++ /dev/null @@ -1,725 +0,0 @@ -nextflow_process { - - name "Test Process FASTP" - script "../main.nf" - process "FASTP" - tag "modules" - tag "modules_nfcore" - tag "fastp" - - test("test_fastp_single_end") { - - when { - params { - outdir = "$outputDir" - } - process { - """ - adapter_fasta = [] - save_trimmed_fail = false - save_merged = false - - input[0] = Channel.of([ - [ id:'test', single_end:true ], - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] - ]) - input[1] = adapter_fasta - input[2] = save_trimmed_fail - input[3] = save_merged - """ - } - } - - then { - def html_text = [ "Q20 bases:") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_single") } - ) - } - } - - test("sarscov2 paired-end [fastq]") { - - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, - { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, - { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, - { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, - { assert path(process.out.html[0][1][0]).text.contains("") }, - { assert path(process.out.html[0][1][1]).text.contains("") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_paired") } - ) - } - } - - test("sarscov2 interleaved [fastq]") { - - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") } + { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" }, + { assert path(process.out.html.get(0).get(1)).getText().contains("") }, + { assert snapshot(process.out.versions).match("versions") }, + { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" } ) } } - - test("sarscov2 paired-end [bam]") { - - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_bam") } - ) - } - } - - test("sarscov2 multiple [fastq]") { - - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, - { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, - { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, - { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, - { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, - { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, - { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, - { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, - { assert path(process.out.html[0][1][0]).text.contains("") }, - { assert path(process.out.html[0][1][1]).text.contains("") }, - { assert path(process.out.html[0][1][2]).text.contains("") }, - { assert path(process.out.html[0][1][3]).text.contains("") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_multiple") } - ) - } - } - - test("sarscov2 custom_prefix") { - - when { - process { - """ - input[0] = Channel.of([ - [ id:'mysample', single_end:true ], // meta map - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") } - ) - } - } - - test("sarscov2 single-end [fastq] - stub") { - - options "-stub" - - when { - process { - """ - input[0] = Channel.of([ - [ id: 'test', single_end:true ], - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - { assert snapshot(process.out.html.collect { file(it[1]).getName() } + - process.out.zip.collect { file(it[1]).getName() } + - process.out.versions ).match("fastqc_stub") } - ) - } - } - -} \ No newline at end of file +// TODO +// // +// // Test with paired-end data +// // +// workflow test_fastqc_paired_end { +// input = [ +// [id: 'test', single_end: false], // meta map +// [ +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) +// ] +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with interleaved data +// // +// workflow test_fastqc_interleaved { +// input = [ +// [id: 'test', single_end: false], // meta map +// file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with bam data +// // +// workflow test_fastqc_bam { +// input = [ +// [id: 'test', single_end: false], // meta map +// file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with multiple samples +// // +// workflow test_fastqc_multiple { +// input = [ +// [id: 'test', single_end: false], // meta map +// [ +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) +// ] +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with custom prefix +// // +// workflow test_fastqc_custom_prefix { +// input = [ +// [ id:'mysample', single_end:true ], // meta map +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } +} diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 86f7c311..636a32ce 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,88 +1,10 @@ { - "fastqc_versions_interleaved": { + "versions": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:40:07.293713" - }, - "fastqc_stub": { - "content": [ - [ - "test.html", - "test.zip", - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:31:01.425198" - }, - "fastqc_versions_multiple": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:40:55.797907" - }, - "fastqc_versions_bam": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:40:26.795862" - }, - "fastqc_versions_single": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:39:27.043675" - }, - "fastqc_versions_paired": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:39:47.584191" - }, - "fastqc_versions_custom_prefix": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:41:14.576531" + "timestamp": "2023-10-09T23:40:54+0000" } } \ No newline at end of file diff --git a/modules/nf-core/gunzip/environment.yml b/modules/nf-core/gunzip/environment.yml deleted file mode 100644 index 25910b34..00000000 --- a/modules/nf-core/gunzip/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: gunzip -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - conda-forge::sed=4.7 diff --git a/modules/nf-core/gunzip/main.nf b/modules/nf-core/gunzip/main.nf deleted file mode 100644 index aea95e75..00000000 --- a/modules/nf-core/gunzip/main.nf +++ /dev/null @@ -1,48 +0,0 @@ -process GUNZIP { - tag "$archive" - label 'process_single' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : - 'quay.io/nf-core/ubuntu:20.04' }" - - input: - tuple val(meta), path(archive) - - output: - tuple val(meta), path("$gunzip"), emit: gunzip - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - gunzip = archive.toString() - '.gz' - """ - # Not calling gunzip itself because it creates files - # with the original group ownership rather than the - # default one for that user / the work directory - gzip \\ - -cd \\ - $args \\ - $archive \\ - > $gunzip - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//') - END_VERSIONS - """ - - stub: - gunzip = archive.toString() - '.gz' - """ - touch $gunzip - cat <<-END_VERSIONS > versions.yml - "${task.process}": - gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//') - END_VERSIONS - """ -} diff --git a/modules/nf-core/gunzip/meta.yml b/modules/nf-core/gunzip/meta.yml deleted file mode 100644 index 231034f2..00000000 --- a/modules/nf-core/gunzip/meta.yml +++ /dev/null @@ -1,39 +0,0 @@ -name: gunzip -description: Compresses and decompresses files. -keywords: - - gunzip - - compression - - decompression -tools: - - gunzip: - description: | - gzip is a file format and a software application used for file compression and decompression. - documentation: https://www.gnu.org/software/gzip/manual/gzip.html - licence: ["GPL-3.0-or-later"] -input: - - meta: - type: map - description: | - Optional groovy Map containing meta information - e.g. [ id:'test', single_end:false ] - - archive: - type: file - description: File to be compressed/uncompressed - pattern: "*.*" -output: - - gunzip: - type: file - description: Compressed/uncompressed file - pattern: "*.*" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@joseespinosa" - - "@drpatelh" - - "@jfy133" -maintainers: - - "@joseespinosa" - - "@drpatelh" - - "@jfy133" diff --git a/modules/nf-core/gunzip/tests/main.nf.test b/modules/nf-core/gunzip/tests/main.nf.test deleted file mode 100644 index 3e9b88b4..00000000 --- a/modules/nf-core/gunzip/tests/main.nf.test +++ /dev/null @@ -1,36 +0,0 @@ -nextflow_process { - - name "Test Process GUNZIP" - script "../main.nf" - process "GUNZIP" - tag "gunzip" - tag "modules_nfcore" - tag "modules" - - test("Should run without failures") { - - when { - params { - outdir = "$outputDir" - } - process { - """ - input[0] = Channel.of([ - [], - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) - ] - ) - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() } - ) - } - - } - -} \ No newline at end of file diff --git a/modules/nf-core/gunzip/tests/main.nf.test.snap b/modules/nf-core/gunzip/tests/main.nf.test.snap deleted file mode 100644 index 720fd9ff..00000000 --- a/modules/nf-core/gunzip/tests/main.nf.test.snap +++ /dev/null @@ -1,31 +0,0 @@ -{ - "Should run without failures": { - "content": [ - { - "0": [ - [ - [ - - ], - "test_1.fastq:md5,4161df271f9bfcd25d5845a1e220dbec" - ] - ], - "1": [ - "versions.yml:md5,54376d32aca20e937a4ec26dac228e84" - ], - "gunzip": [ - [ - [ - - ], - "test_1.fastq:md5,4161df271f9bfcd25d5845a1e220dbec" - ] - ], - "versions": [ - "versions.yml:md5,54376d32aca20e937a4ec26dac228e84" - ] - } - ], - "timestamp": "2023-10-17T15:35:37.690477896" - } -} \ No newline at end of file diff --git a/modules/nf-core/gunzip/tests/tags.yml b/modules/nf-core/gunzip/tests/tags.yml deleted file mode 100644 index fd3f6915..00000000 --- a/modules/nf-core/gunzip/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -gunzip: - - modules/nf-core/gunzip/** diff --git a/modules/nf-core/fastp/environment.yml b/modules/nf-core/multiqc/environment.yml similarity index 61% rename from modules/nf-core/fastp/environment.yml rename to modules/nf-core/multiqc/environment.yml index 70389e66..bc0bdb5b 100644 --- a/modules/nf-core/fastp/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -1,7 +1,7 @@ -name: fastp +name: multiqc channels: - conda-forge - bioconda - defaults dependencies: - - bioconda::fastp=0.23.4 + - bioconda::multiqc=1.18 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf new file mode 100644 index 00000000..00cc48d2 --- /dev/null +++ b/modules/nf-core/multiqc/main.nf @@ -0,0 +1,55 @@ +process MULTIQC { + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/multiqc:1.18--pyhdfd78af_0' : + 'biocontainers/multiqc:1.18--pyhdfd78af_0' }" + + input: + path multiqc_files, stageAs: "?/*" + path(multiqc_config) + path(extra_multiqc_config) + path(multiqc_logo) + + output: + path "*multiqc_report.html", emit: report + path "*_data" , emit: data + path "*_plots" , optional:true, emit: plots + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def config = multiqc_config ? "--config $multiqc_config" : '' + def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : '' + def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : '' + """ + multiqc \\ + --force \\ + $args \\ + $config \\ + $extra_config \\ + $logo \\ + . + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" ) + END_VERSIONS + """ + + stub: + """ + touch multiqc_data + touch multiqc_plots + touch multiqc_report.html + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" ) + END_VERSIONS + """ +} diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml new file mode 100644 index 00000000..f1aa660e --- /dev/null +++ b/modules/nf-core/multiqc/meta.yml @@ -0,0 +1,59 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: multiqc +description: Aggregate results from bioinformatics analyses across many samples into a single report +keywords: + - QC + - bioinformatics tools + - Beautiful stand-alone HTML report +tools: + - multiqc: + description: | + MultiQC searches a given directory for analysis logs and compiles a HTML report. + It's a general use tool, perfect for summarising the output from numerous bioinformatics tools. + homepage: https://multiqc.info/ + documentation: https://multiqc.info/docs/ + licence: ["GPL-3.0-or-later"] +input: + - multiqc_files: + type: file + description: | + List of reports / files recognised by MultiQC, for example the html and zip output of FastQC + - multiqc_config: + type: file + description: Optional config yml for MultiQC + pattern: "*.{yml,yaml}" + - extra_multiqc_config: + type: file + description: Second optional config yml for MultiQC. Will override common sections in multiqc_config. + pattern: "*.{yml,yaml}" + - multiqc_logo: + type: file + description: Optional logo file for MultiQC + pattern: "*.{png}" +output: + - report: + type: file + description: MultiQC report file + pattern: "multiqc_report.html" + - data: + type: directory + description: MultiQC data dir + pattern: "multiqc_data" + - plots: + type: file + description: Plots created by MultiQC + pattern: "*_data" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@abhi18av" + - "@bunop" + - "@drpatelh" + - "@jfy133" +maintainers: + - "@abhi18av" + - "@bunop" + - "@drpatelh" + - "@jfy133" diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test new file mode 100644 index 00000000..c2dad217 --- /dev/null +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -0,0 +1,63 @@ +nextflow_process { + + name "Test Process MULTIQC" + script "../main.nf" + process "MULTIQC" + tag "modules" + tag "modules_nfcore" + tag "multiqc" + + test("MULTIQC: FASTQC") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert path(process.out.report.get(0)).exists() }, + { assert path(process.out.data.get(0)).exists() }, + { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + ) + } + + } + + test("MULTIQC: FASTQC and a config file") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert path(process.out.report.get(0)).exists() }, + { assert path(process.out.data.get(0)).exists() }, + { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + ) + } + + } +} diff --git a/modules/nf-core/multiqc/tests/tags.yml b/modules/nf-core/multiqc/tests/tags.yml new file mode 100644 index 00000000..bea6c0d3 --- /dev/null +++ b/modules/nf-core/multiqc/tests/tags.yml @@ -0,0 +1,2 @@ +multiqc: + - modules/nf-core/multiqc/** diff --git a/modules/nf-core/seqkit/seq/environment.yml b/modules/nf-core/seqkit/seq/environment.yml deleted file mode 100644 index 9019d269..00000000 --- a/modules/nf-core/seqkit/seq/environment.yml +++ /dev/null @@ -1,9 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json -name: "seqkit_seq" -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - "bioconda::seqkit=2.6.1" diff --git a/modules/nf-core/seqkit/seq/main.nf b/modules/nf-core/seqkit/seq/main.nf deleted file mode 100644 index d05da23d..00000000 --- a/modules/nf-core/seqkit/seq/main.nf +++ /dev/null @@ -1,63 +0,0 @@ -process SEQKIT_SEQ { - tag "$meta.id" - label 'process_low' - // File IO can be a bottleneck. See: https://bioinf.shenwei.me/seqkit/usage/#parallelization-of-cpu-intensive-jobs - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/seqkit:2.6.1--h9ee0642_0': - 'quay.io/biocontainers/seqkit:2.6.1--h9ee0642_0' }" - - input: - tuple val(meta), path(fastx) - - output: - tuple val(meta), path("${prefix}.*") , emit: fastx - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def args2 = task.ext.args2 ?: '' - prefix = task.ext.prefix ?: "${meta.id}" - def extension = "fastq" - if ("$fastx" ==~ /.+\.fasta|.+\.fasta.gz|.+\.fa|.+\.fa.gz|.+\.fas|.+\.fas.gz|.+\.fna|.+\.fna.gz|.+\.fsa|.+\.fsa.gz/ ) { - extension = "fasta" - } - extension = fastx.toString().endsWith('.gz') ? "${extension}.gz" : extension - def call_gzip = extension.endsWith('.gz') ? "| gzip -c $args2" : '' - if("${prefix}.${extension}" == "$fastx") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" - """ - seqkit \\ - seq \\ - --threads $task.cpus \\ - $args \\ - $fastx \\ - $call_gzip \\ - > ${prefix}.${extension} - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - seqkit: \$(seqkit version | cut -d' ' -f2) - END_VERSIONS - """ - - stub: - prefix = task.ext.prefix ?: "${meta.id}" - def extension = "fastq" - if ("$fastx" ==~ /.+\.fasta|.+\.fasta.gz|.+\.fa|.+\.fa.gz|.+\.fas|.+\.fas.gz|.+\.fna|.+\.fna.gz|.+\.fsa|.+\.fsa.gz/ ) { - extension = "fasta" - } - extension = fastx.toString().endsWith('.gz') ? "${extension}.gz" : extension - if("${prefix}.${extension}" == "$fastx") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" - """ - touch ${prefix}.${extension} - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - seqkit: \$(seqkit version | cut -d' ' -f2) - END_VERSIONS - """ -} diff --git a/modules/nf-core/seqkit/seq/meta.yml b/modules/nf-core/seqkit/seq/meta.yml deleted file mode 100644 index 8d4e2b16..00000000 --- a/modules/nf-core/seqkit/seq/meta.yml +++ /dev/null @@ -1,48 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: "seqkit_seq" -description: Transforms sequences (extract ID, filter by length, remove gaps, reverse complement...) -keywords: - - genomics - - fasta - - fastq - - transform - - filter - - gaps - - complement -tools: - - "seqkit": - description: "A cross-platform and ultrafast toolkit for FASTA/Q file manipulation" - homepage: "https://bioinf.shenwei.me/seqkit/" - documentation: "https://bioinf.shenwei.me/seqkit/usage/" - tool_dev_url: "https://github.com/shenwei356/seqkit" - doi: "10.1371/journal.pone.0163962" - licence: ["MIT"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - fastx: - type: file - description: Input fasta/fastq file - pattern: "*.{fsa,fas,fa,fasta,fastq,fq,fsa.gz,fas.gz,fa.gz,fasta.gz,fastq.gz,fq.gz}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - fastx: - type: file - description: Output fasta/fastq file - pattern: "*.{fasta,fasta.gz,fastq,fastq.gz}" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@GallVp" -maintainers: - - "@GallVp" diff --git a/modules/nf-core/seqkit/seq/tests/main.nf.test b/modules/nf-core/seqkit/seq/tests/main.nf.test deleted file mode 100644 index 304d1a67..00000000 --- a/modules/nf-core/seqkit/seq/tests/main.nf.test +++ /dev/null @@ -1,149 +0,0 @@ -nextflow_process { - - name "Test Process SEQKIT_SEQ" - script "../main.nf" - process "SEQKIT_SEQ" - config './nextflow.config' - - tag "modules" - tag "modules_nfcore" - tag "seqkit" - tag "seqkit/seq" - - test("sarscov2-genome_fasta") { - when { - process { - """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - - test("sarscov2-genome_fasta_gz") { - when { - process { - """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['sarscov2']['genome']['genome_fasta_gz'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - - test("sarscov2-test_1_fastq_gz") { - when { - process { - """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - - test("file_name_conflict-fail_with_error") { - when { - process { - """ - input[0] = [ - [ id:'test_1' ], // meta map - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert !process.success }, - { assert process.stdout.toString().contains("Input and output names are the same") } - ) - } - - } - - test("sarscov2-genome_fasta-stub") { - - options '-stub' - - when { - process { - """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - - test("file_name_conflict-fail_with_error-stub") { - - options '-stub' - - when { - process { - """ - input[0] = [ - [ id:'genome' ], // meta map - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert !process.success }, - { assert process.stdout.toString().contains("Input and output names are the same") } - ) - } - - } - -} \ No newline at end of file diff --git a/modules/nf-core/seqkit/seq/tests/main.nf.test.snap b/modules/nf-core/seqkit/seq/tests/main.nf.test.snap deleted file mode 100644 index 91b4d17a..00000000 --- a/modules/nf-core/seqkit/seq/tests/main.nf.test.snap +++ /dev/null @@ -1,126 +0,0 @@ -{ - "sarscov2-genome_fasta-stub": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.fasta:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ], - "1": [ - "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" - ], - "fastx": [ - [ - { - "id": "test" - }, - "test.fasta:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ], - "versions": [ - "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" - ] - } - ], - "timestamp": "2023-12-18T10:34:00.37449" - }, - "versions": { - "content": [ - [ - "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" - ] - ], - "timestamp": "2023-12-17T13:56:53.318962" - }, - "sarscov2-test_1_fastq_gz": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.fastq.gz:md5,4161df271f9bfcd25d5845a1e220dbec" - ] - ], - "1": [ - "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" - ], - "fastx": [ - [ - { - "id": "test" - }, - "test.fastq.gz:md5,4161df271f9bfcd25d5845a1e220dbec" - ] - ], - "versions": [ - "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" - ] - } - ], - "timestamp": "2023-12-18T10:33:53.528342" - }, - "sarscov2-genome_fasta": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.fasta:md5,483f4a5dfe60171c86ee9b7e6dff908b" - ] - ], - "1": [ - "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" - ], - "fastx": [ - [ - { - "id": "test" - }, - "test.fasta:md5,483f4a5dfe60171c86ee9b7e6dff908b" - ] - ], - "versions": [ - "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" - ] - } - ], - "timestamp": "2023-12-18T10:33:44.757686" - }, - "sarscov2-genome_fasta_gz": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.fasta.gz:md5,483f4a5dfe60171c86ee9b7e6dff908b" - ] - ], - "1": [ - "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" - ], - "fastx": [ - [ - { - "id": "test" - }, - "test.fasta.gz:md5,483f4a5dfe60171c86ee9b7e6dff908b" - ] - ], - "versions": [ - "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" - ] - } - ], - "timestamp": "2023-12-18T10:33:49.115171" - } -} \ No newline at end of file diff --git a/modules/nf-core/seqkit/seq/tests/nextflow.config b/modules/nf-core/seqkit/seq/tests/nextflow.config deleted file mode 100644 index d8e3c66a..00000000 --- a/modules/nf-core/seqkit/seq/tests/nextflow.config +++ /dev/null @@ -1,3 +0,0 @@ -process { - ext.args2 = '-n' -} diff --git a/modules/nf-core/seqkit/seq/tests/tags.yml b/modules/nf-core/seqkit/seq/tests/tags.yml deleted file mode 100644 index 5eeca7e3..00000000 --- a/modules/nf-core/seqkit/seq/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -seqkit/seq: - - "modules/nf-core/seqkit/seq/**" diff --git a/modules/nf-core/seqkit/sort/environment.yml b/modules/nf-core/seqkit/sort/environment.yml deleted file mode 100644 index 820c5707..00000000 --- a/modules/nf-core/seqkit/sort/environment.yml +++ /dev/null @@ -1,9 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json -name: "seqkit_sort" -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - "bioconda::seqkit=2.6.1" diff --git a/modules/nf-core/seqkit/sort/main.nf b/modules/nf-core/seqkit/sort/main.nf deleted file mode 100644 index ae2485e1..00000000 --- a/modules/nf-core/seqkit/sort/main.nf +++ /dev/null @@ -1,63 +0,0 @@ -process SEQKIT_SORT { - tag "$meta.id" - label 'process_low' - // File IO can be a bottleneck. See: https://bioinf.shenwei.me/seqkit/usage/#parallelization-of-cpu-intensive-jobs - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/seqkit:2.6.1--h9ee0642_0': - 'quay.io/biocontainers/seqkit:2.6.1--h9ee0642_0' }" - - input: - tuple val(meta), path(fastx) - - output: - tuple val(meta), path("${prefix}.*") , emit: fastx - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def args2 = task.ext.args2 ?: '' - prefix = task.ext.prefix ?: "${meta.id}" - def extension = "fastq" - if ("$fastx" ==~ /.+\.fasta|.+\.fasta.gz|.+\.fa|.+\.fa.gz|.+\.fas|.+\.fas.gz|.+\.fna|.+\.fna.gz|.+\.fsa|.+\.fsa.gz/ ) { - extension = "fasta" - } - extension = fastx.toString().endsWith('.gz') ? "${extension}.gz" : extension - def call_gzip = extension.endsWith('.gz') ? "| gzip -c $args2 " : '' - if("${prefix}.${extension}" == "$fastx") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" - """ - seqkit \\ - sort \\ - --threads $task.cpus \\ - $args \\ - $fastx \\ - $call_gzip \\ - > ${prefix}.${extension} - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - seqkit: \$(seqkit version | cut -d' ' -f2) - END_VERSIONS - """ - - stub: - prefix = task.ext.prefix ?: "${meta.id}" - def extension = "fastq" - if ("$fastx" ==~ /.+\.fasta|.+\.fasta.gz|.+\.fa|.+\.fa.gz|.+\.fas|.+\.fas.gz|.+\.fna|.+\.fna.gz|.+\.fsa|.+\.fsa.gz/ ) { - extension = "fasta" - } - extension = fastx.toString().endsWith('.gz') ? "${extension}.gz" : extension - if("${prefix}.${extension}" == "$fastx") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" - """ - touch ${prefix}.${extension} - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - seqkit: \$(seqkit version | cut -d' ' -f2) - END_VERSIONS - """ -} diff --git a/modules/nf-core/seqkit/sort/meta.yml b/modules/nf-core/seqkit/sort/meta.yml deleted file mode 100644 index 2e61ce15..00000000 --- a/modules/nf-core/seqkit/sort/meta.yml +++ /dev/null @@ -1,45 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: "seqkit_sort" -description: Sorts sequences by id/name/sequence/length -keywords: - - genomics - - fasta - - fastq - - sort -tools: - - "seqkit": - description: "A cross-platform and ultrafast toolkit for FASTA/Q file manipulation" - homepage: "https://bioinf.shenwei.me/seqkit/" - documentation: "https://bioinf.shenwei.me/seqkit/usage/" - tool_dev_url: "https://github.com/shenwei356/seqkit" - doi: "10.1371/journal.pone.0163962" - licence: ["MIT"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - fastx: - type: file - description: Input fasta/fastq file - pattern: "*.{fsa,fas,fa,fasta,fastq,fq,fsa.gz,fas.gz,fa.gz,fasta.gz,fastq.gz,fq.gz}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - fastx: - type: file - description: Output fasta/fastq file - pattern: "*.{fasta.gz,fastq.gz}" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@GallVp" -maintainers: - - "@GallVp" diff --git a/modules/nf-core/seqkit/sort/tests/main.nf.test b/modules/nf-core/seqkit/sort/tests/main.nf.test deleted file mode 100644 index 19857b32..00000000 --- a/modules/nf-core/seqkit/sort/tests/main.nf.test +++ /dev/null @@ -1,149 +0,0 @@ -nextflow_process { - - name "Test Process SEQKIT_SORT" - script "../main.nf" - process "SEQKIT_SORT" - config './nextflow.config' - - tag "modules" - tag "modules_nfcore" - tag "seqkit" - tag "seqkit/sort" - - test("sarscov2-genome_fasta") { - when { - process { - """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - - test("sarscov2-genome_fasta_gz") { - when { - process { - """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['sarscov2']['genome']['genome_fasta_gz'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - - test("sarscov2-test_1_fastq_gz") { - when { - process { - """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - - test("file_name_conflict-fail_with_error") { - when { - process { - """ - input[0] = [ - [ id:'test_1' ], // meta map - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert !process.success }, - { assert process.stdout.toString().contains("Input and output names are the same,") } - ) - } - - } - - test("sarscov2-genome_fasta-stub") { - - options '-stub' - - when { - process { - """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - - test("file_name_conflict-fail_with_error-stub") { - - options '-stub' - - when { - process { - """ - input[0] = [ - [ id:'genome' ], // meta map - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert !process.success }, - { assert process.stdout.toString().contains("Input and output names are the same") } - ) - } - - } - -} \ No newline at end of file diff --git a/modules/nf-core/seqkit/sort/tests/main.nf.test.snap b/modules/nf-core/seqkit/sort/tests/main.nf.test.snap deleted file mode 100644 index 94e2cb35..00000000 --- a/modules/nf-core/seqkit/sort/tests/main.nf.test.snap +++ /dev/null @@ -1,126 +0,0 @@ -{ - "sarscov2-genome_fasta-stub": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.fasta:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ], - "1": [ - "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" - ], - "fastx": [ - [ - { - "id": "test" - }, - "test.fasta:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ], - "versions": [ - "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" - ] - } - ], - "timestamp": "2023-12-18T10:07:19.28815" - }, - "versions": { - "content": [ - [ - "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" - ] - ], - "timestamp": "2023-12-18T10:07:15.341516" - }, - "sarscov2-test_1_fastq_gz": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.fastq.gz:md5,80d9fd1976648214f44e42bc3d705972" - ] - ], - "1": [ - "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" - ], - "fastx": [ - [ - { - "id": "test" - }, - "test.fastq.gz:md5,80d9fd1976648214f44e42bc3d705972" - ] - ], - "versions": [ - "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" - ] - } - ], - "timestamp": "2023-12-18T10:12:25.704893" - }, - "sarscov2-genome_fasta": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.fasta:md5,483f4a5dfe60171c86ee9b7e6dff908b" - ] - ], - "1": [ - "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" - ], - "fastx": [ - [ - { - "id": "test" - }, - "test.fasta:md5,483f4a5dfe60171c86ee9b7e6dff908b" - ] - ], - "versions": [ - "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" - ] - } - ], - "timestamp": "2023-12-18T10:07:15.293713" - }, - "sarscov2-genome_fasta_gz": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.fasta.gz:md5,483f4a5dfe60171c86ee9b7e6dff908b" - ] - ], - "1": [ - "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" - ], - "fastx": [ - [ - { - "id": "test" - }, - "test.fasta.gz:md5,483f4a5dfe60171c86ee9b7e6dff908b" - ] - ], - "versions": [ - "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" - ] - } - ], - "timestamp": "2023-12-18T10:09:20.306713" - } -} \ No newline at end of file diff --git a/modules/nf-core/seqkit/sort/tests/nextflow.config b/modules/nf-core/seqkit/sort/tests/nextflow.config deleted file mode 100644 index d8e3c66a..00000000 --- a/modules/nf-core/seqkit/sort/tests/nextflow.config +++ /dev/null @@ -1,3 +0,0 @@ -process { - ext.args2 = '-n' -} diff --git a/modules/nf-core/seqkit/sort/tests/tags.yml b/modules/nf-core/seqkit/sort/tests/tags.yml deleted file mode 100644 index c839b58c..00000000 --- a/modules/nf-core/seqkit/sort/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -seqkit/sort: - - "modules/nf-core/seqkit/sort/**" diff --git a/modules/nf-core/tidk/explore/environment.yml b/modules/nf-core/tidk/explore/environment.yml deleted file mode 100644 index 4fe55407..00000000 --- a/modules/nf-core/tidk/explore/environment.yml +++ /dev/null @@ -1,9 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json -name: "tidk_explore" -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - "bioconda::tidk=0.2.41" diff --git a/modules/nf-core/tidk/explore/main.nf b/modules/nf-core/tidk/explore/main.nf deleted file mode 100644 index c8baeca8..00000000 --- a/modules/nf-core/tidk/explore/main.nf +++ /dev/null @@ -1,57 +0,0 @@ -process TIDK_EXPLORE { - tag "$meta.id" - label 'process_single' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/tidk:0.2.41--hdbdd923_0': - 'quay.io/biocontainers/tidk:0.2.41--hdbdd923_0' }" - - input: - tuple val(meta), path(fasta) - - output: - tuple val(meta), path("*.tidk.explore.tsv") , emit: explore_tsv - tuple val(meta), path("*.top.sequence.txt") , emit: top_sequence, optional: true - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - """ - tidk \\ - explore \\ - $args \\ - $fasta \\ - > ${prefix}.tidk.explore.tsv - - [[ \$(cat ${prefix}.tidk.explore.tsv | wc -l) -gt 1 ]] \\ - && cat \\ - ${prefix}.tidk.explore.tsv \\ - | sed -n 2p \\ - | awk '{print \$1;}' \\ - > ${prefix}.top.sequence.txt \\ - || echo "No sequence identified" - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - tidk: \$(tidk --version | sed 's/tidk //') - END_VERSIONS - """ - - stub: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.tidk.explore.tsv - touch ${prefix}.top.sequence.txt - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - tidk: \$(tidk --version | sed 's/tidk //') - END_VERSIONS - """ -} diff --git a/modules/nf-core/tidk/explore/meta.yml b/modules/nf-core/tidk/explore/meta.yml deleted file mode 100644 index 582aaf56..00000000 --- a/modules/nf-core/tidk/explore/meta.yml +++ /dev/null @@ -1,52 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: "tidk_explore" -description: | - `tidk explore` attempts to find the simple telomeric repeat unit in the genome provided. - It will report this repeat in its canonical form (e.g. TTAGG -> AACCT). -keywords: - - genomics - - telomere - - search -tools: - - "tidk": - description: tidk is a toolkit to identify and visualise telomeric repeats in genomes - homepage: "https://github.com/tolkit/telomeric-identifier" - documentation: "https://github.com/tolkit/telomeric-identifier" - tool_dev_url: "https://github.com/tolkit/telomeric-identifier" - doi: "10.5281/zenodo.10091385" - licence: ["MIT"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - fasta: - type: file - description: The input fasta file - pattern: "*.{fsa,fa,fasta}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - explore_tsv: - type: file - description: Telomeres and their frequencies in TSV format - pattern: "*.tidk.explore.tsv" - - top_sequence: - type: file - description: | - The most frequent telomere sequence if one or more - sequences are identified by the toolkit - pattern: "*.top.sequence.txt" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@GallVp" -maintainers: - - "@GallVp" diff --git a/modules/nf-core/tidk/explore/tests/main.nf.test b/modules/nf-core/tidk/explore/tests/main.nf.test deleted file mode 100644 index 9f00f522..00000000 --- a/modules/nf-core/tidk/explore/tests/main.nf.test +++ /dev/null @@ -1,86 +0,0 @@ -nextflow_process { - - name "Test Process TIDK_EXPLORE" - script "../main.nf" - process "TIDK_EXPLORE" - config "./nextflow.config" - - tag "modules" - tag "modules_nfcore" - tag "tidk" - tag "tidk/explore" - - test("homo_sapiens-genome_21_fasta-success") { - - when { - process { - """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - - test("homo_sapiens-genome_fasta-no_top_sequence") { - - when { - process { - """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out.explore_tsv).match("no_top_sequence_explore_tsv") }, - { assert process.out.top_sequence == [] }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - - test("stub") { - - options '-stub' - - when { - process { - """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert process.out.explore_tsv != null }, - { assert process.out.top_sequence != null }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - -} \ No newline at end of file diff --git a/modules/nf-core/tidk/explore/tests/main.nf.test.snap b/modules/nf-core/tidk/explore/tests/main.nf.test.snap deleted file mode 100644 index c46d31c1..00000000 --- a/modules/nf-core/tidk/explore/tests/main.nf.test.snap +++ /dev/null @@ -1,68 +0,0 @@ -{ - "homo_sapiens-genome_21_fasta-success": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.tidk.explore.tsv:md5,89de91ef36eb0925aefca61757f5275f" - ] - ], - "1": [ - [ - { - "id": "test" - }, - "test.top.sequence.txt:md5,bd63900958df06516e45b887072d788f" - ] - ], - "2": [ - "versions.yml:md5,8de27958aee1d1fc9075e8046114bdb5" - ], - "explore_tsv": [ - [ - { - "id": "test" - }, - "test.tidk.explore.tsv:md5,89de91ef36eb0925aefca61757f5275f" - ] - ], - "top_sequence": [ - [ - { - "id": "test" - }, - "test.top.sequence.txt:md5,bd63900958df06516e45b887072d788f" - ] - ], - "versions": [ - "versions.yml:md5,8de27958aee1d1fc9075e8046114bdb5" - ] - } - ], - "timestamp": "2023-12-05T14:13:24.14906" - }, - "no_top_sequence_explore_tsv": { - "content": [ - [ - [ - { - "id": "test" - }, - "test.tidk.explore.tsv:md5,27b8513be5478ea2b846a407b3639c01" - ] - ] - ], - "timestamp": "2023-12-05T14:16:18.982423" - }, - "versions": { - "content": [ - [ - "versions.yml:md5,8de27958aee1d1fc9075e8046114bdb5" - ] - ], - "timestamp": "2023-12-05T14:13:24.198311" - } -} \ No newline at end of file diff --git a/modules/nf-core/tidk/explore/tests/nextflow.config b/modules/nf-core/tidk/explore/tests/nextflow.config deleted file mode 100644 index d8f33fe0..00000000 --- a/modules/nf-core/tidk/explore/tests/nextflow.config +++ /dev/null @@ -1,3 +0,0 @@ -process { - ext.args = '--minimum 5 --maximum 30' -} diff --git a/modules/nf-core/tidk/explore/tests/tags.yml b/modules/nf-core/tidk/explore/tests/tags.yml deleted file mode 100644 index af666655..00000000 --- a/modules/nf-core/tidk/explore/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -tidk/explore: - - "modules/nf-core/tidk/explore/**" diff --git a/modules/nf-core/tidk/plot/environment.yml b/modules/nf-core/tidk/plot/environment.yml deleted file mode 100644 index ed1706e3..00000000 --- a/modules/nf-core/tidk/plot/environment.yml +++ /dev/null @@ -1,9 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json -name: "tidk_plot" -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - "bioconda::tidk=0.2.41" diff --git a/modules/nf-core/tidk/plot/main.nf b/modules/nf-core/tidk/plot/main.nf deleted file mode 100644 index 0282f1c9..00000000 --- a/modules/nf-core/tidk/plot/main.nf +++ /dev/null @@ -1,47 +0,0 @@ -process TIDK_PLOT { - tag "$meta.id" - label 'process_single' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/tidk:0.2.41--hdbdd923_0': - 'quay.io/biocontainers/tidk:0.2.41--hdbdd923_0' }" - - input: - tuple val(meta), path(tsv) - - output: - tuple val(meta), path("*.svg"), emit: svg - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - """ - tidk \\ - plot \\ - --output $prefix \\ - $args \\ - --tsv "$tsv" - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - tidk: \$(tidk --version | sed 's/tidk //') - END_VERSIONS - """ - - stub: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.svg - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - tidk: \$(tidk --version | sed 's/tidk //') - END_VERSIONS - """ -} diff --git a/modules/nf-core/tidk/plot/meta.yml b/modules/nf-core/tidk/plot/meta.yml deleted file mode 100644 index 451195c8..00000000 --- a/modules/nf-core/tidk/plot/meta.yml +++ /dev/null @@ -1,47 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: "tidk_plot" -description: | - Plots telomeric repeat frequency against sliding window location - using data produced by `tidk/search` -keywords: - - genomics - - telomere - - search - - plot -tools: - - "tidk": - description: tidk is a toolkit to identify and visualise telomeric repeats in genomes - homepage: "https://github.com/tolkit/telomeric-identifier" - documentation: "https://github.com/tolkit/telomeric-identifier" - tool_dev_url: "https://github.com/tolkit/telomeric-identifier" - doi: "10.5281/zenodo.10091385" - licence: ["MIT"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - tsv: - type: file - description: Search results in TSV format from `tidk search` - pattern: "*.tsv" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - svg: - type: file - description: Telomere search plot - pattern: "*.svg" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@GallVp" -maintainers: - - "@GallVp" diff --git a/modules/nf-core/tidk/plot/tests/main.nf.test b/modules/nf-core/tidk/plot/tests/main.nf.test deleted file mode 100644 index 179c537e..00000000 --- a/modules/nf-core/tidk/plot/tests/main.nf.test +++ /dev/null @@ -1,74 +0,0 @@ -nextflow_process { - - name "Test Process TIDK_PLOT" - script "../main.nf" - process "TIDK_PLOT" - - tag "modules" - tag "modules_nfcore" - tag "tidk" - tag "tidk/search" - tag "tidk/plot" - - test("homo_sapiens-genome_21_fasta-success") { - - setup { - run("TIDK_SEARCH") { - script "../../../../nf-core/tidk/search" - - process { - """ - input[0] = [ - [ id:'test' ], - file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) - ] - input[1] = 'TTAGGG' - """ - } - } - } - - when { - process { - """ - input[0] = TIDK_SEARCH.out.tsv - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - - test("stub") { - - options '-stub' - - when { - process { - """ - input[0] = [ - [ id:'test' ], - [] - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - -} \ No newline at end of file diff --git a/modules/nf-core/tidk/plot/tests/main.nf.test.snap b/modules/nf-core/tidk/plot/tests/main.nf.test.snap deleted file mode 100644 index 5de9533e..00000000 --- a/modules/nf-core/tidk/plot/tests/main.nf.test.snap +++ /dev/null @@ -1,68 +0,0 @@ -{ - "homo_sapiens-genome_21_fasta-success": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.svg:md5,e76985fdc220867a05967984c2c4675d" - ] - ], - "1": [ - "versions.yml:md5,b850b0d8b9add80d7863cc62e3f32c99" - ], - "svg": [ - [ - { - "id": "test" - }, - "test.svg:md5,e76985fdc220867a05967984c2c4675d" - ] - ], - "versions": [ - "versions.yml:md5,b850b0d8b9add80d7863cc62e3f32c99" - ] - } - ], - "timestamp": "2023-12-05T14:29:24.721706" - }, - "versions": { - "content": [ - [ - "versions.yml:md5,b850b0d8b9add80d7863cc62e3f32c99" - ] - ], - "timestamp": "2023-12-05T14:29:55.412674" - }, - "stub": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.svg:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ], - "1": [ - "versions.yml:md5,b850b0d8b9add80d7863cc62e3f32c99" - ], - "svg": [ - [ - { - "id": "test" - }, - "test.svg:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ], - "versions": [ - "versions.yml:md5,b850b0d8b9add80d7863cc62e3f32c99" - ] - } - ], - "timestamp": "2023-12-16T18:50:29.344319" - } -} \ No newline at end of file diff --git a/modules/nf-core/tidk/plot/tests/tags.yml b/modules/nf-core/tidk/plot/tests/tags.yml deleted file mode 100644 index 91228695..00000000 --- a/modules/nf-core/tidk/plot/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -tidk/plot: - - "modules/nf-core/tidk/plot/**" diff --git a/modules/nf-core/tidk/search/environment.yml b/modules/nf-core/tidk/search/environment.yml deleted file mode 100644 index eaf92acc..00000000 --- a/modules/nf-core/tidk/search/environment.yml +++ /dev/null @@ -1,9 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json -name: "tidk_search" -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - "bioconda::tidk=0.2.41" diff --git a/modules/nf-core/tidk/search/main.nf b/modules/nf-core/tidk/search/main.nf deleted file mode 100644 index 20f402e2..00000000 --- a/modules/nf-core/tidk/search/main.nf +++ /dev/null @@ -1,62 +0,0 @@ -process TIDK_SEARCH { - tag "$meta.id" - label 'process_single' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/tidk:0.2.41--hdbdd923_0': - 'quay.io/biocontainers/tidk:0.2.41--hdbdd923_0' }" - - input: - tuple val(meta), path(fasta) - val string - - output: - tuple val(meta), path("*.tsv") , emit: tsv , optional: true - tuple val(meta), path("*.bedgraph") , emit: bedgraph , optional: true - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - """ - tidk \\ - search \\ - --string $string \\ - --output $prefix \\ - --dir tidk \\ - $args \\ - $fasta - - mv \\ - tidk/${prefix}_telomeric_repeat_windows.tsv \\ - ${prefix}.tsv \\ - || echo "TSV file was not produced" - - mv \\ - tidk/${prefix}_telomeric_repeat_windows.bedgraph \\ - ${prefix}.bedgraph \\ - || echo "BEDGRAPH file was not produced" - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - tidk: \$(tidk --version | sed 's/tidk //') - END_VERSIONS - """ - - stub: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def extension = args.contains("--extension bedgraph") ? 'bedgraph' : 'tsv' - """ - touch ${prefix}.${extension} - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - tidk: \$(tidk --version | sed 's/tidk //') - END_VERSIONS - """ -} diff --git a/modules/nf-core/tidk/search/meta.yml b/modules/nf-core/tidk/search/meta.yml deleted file mode 100644 index 8ba07350..00000000 --- a/modules/nf-core/tidk/search/meta.yml +++ /dev/null @@ -1,51 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: "tidk_search" -description: Searches a genome for a telomere string such as TTAGGG -keywords: - - genomics - - telomere - - search -tools: - - "tidk": - description: tidk is a toolkit to identify and visualise telomeric repeats in genomes - homepage: "https://github.com/tolkit/telomeric-identifier" - documentation: "https://github.com/tolkit/telomeric-identifier" - tool_dev_url: "https://github.com/tolkit/telomeric-identifier" - doi: "10.5281/zenodo.10091385" - licence: ["MIT"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - fasta: - type: file - description: The input fasta file - pattern: "*.{fsa,fa,fasta}" - - string: - type: string - description: Search string such as TTAGGG -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - tsv: - type: file - description: Search results in TSV format - pattern: "*.tsv" - - bedgraph: - type: file - description: Search results in BEDGRAPH format - pattern: "*.bedgraph" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@GallVp" -maintainers: - - "@GallVp" diff --git a/modules/nf-core/tidk/search/tests/main.nf.test b/modules/nf-core/tidk/search/tests/main.nf.test deleted file mode 100644 index 64e1d3a5..00000000 --- a/modules/nf-core/tidk/search/tests/main.nf.test +++ /dev/null @@ -1,119 +0,0 @@ -nextflow_process { - - name "Test Process TIDK_SEARCH" - script "../main.nf" - process "TIDK_SEARCH" - - tag "modules" - tag "modules_nfcore" - tag "tidk" - tag "tidk/search" - - test("homo_sapiens-genome_fasta-bedgraph") { - - config "./nextflow.config" - - when { - process { - """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) - ] - input[1] = 'TTAGGG' - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out.bedgraph).match("bedgraph") }, - { assert snapshot(process.out.versions).match("versions") }, - { assert process.out.tsv == [] } - ) - } - - } - - test("homo_sapiens-genome_fasta-tsv") { - - when { - process { - """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) - ] - input[1] = 'TTAGGG' - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out.tsv).match("tsv") }, - { assert snapshot(process.out.versions).match("versions") }, - { assert process.out.bedgraph == [] } - ) - } - - } - - test("stub-bedgraph") { - - options '-stub' - config "./nextflow.config" - - when { - process { - """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) - ] - input[1] = 'TTAGGG' - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert process.out.bedgraph != null }, - { assert process.out.tsv == [] }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - - test("stub-tsv") { - - options '-stub' - - when { - process { - """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) - ] - input[1] = 'TTAGGG' - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert process.out.bedgraph == [] }, - { assert process.out.tsv != null }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - -} \ No newline at end of file diff --git a/modules/nf-core/tidk/search/tests/main.nf.test.snap b/modules/nf-core/tidk/search/tests/main.nf.test.snap deleted file mode 100644 index 15aedd25..00000000 --- a/modules/nf-core/tidk/search/tests/main.nf.test.snap +++ /dev/null @@ -1,36 +0,0 @@ -{ - "bedgraph": { - "content": [ - [ - [ - { - "id": "test" - }, - "test.bedgraph:md5,8277cc74bf083f51584ba6d2b8a8013f" - ] - ] - ], - "timestamp": "2023-12-05T09:53:52.893139" - }, - "versions": { - "content": [ - [ - "versions.yml:md5,6baeeb0e50c9bea6975173a329179f8e" - ] - ], - "timestamp": "2023-12-05T09:53:52.89726" - }, - "tsv": { - "content": [ - [ - [ - { - "id": "test" - }, - "test.tsv:md5,5b44a89396f412eb571ea240ef9deedd" - ] - ] - ], - "timestamp": "2023-12-05T09:53:56.455271" - } -} \ No newline at end of file diff --git a/modules/nf-core/tidk/search/tests/nextflow.config b/modules/nf-core/tidk/search/tests/nextflow.config deleted file mode 100644 index 5d36acfb..00000000 --- a/modules/nf-core/tidk/search/tests/nextflow.config +++ /dev/null @@ -1,3 +0,0 @@ -process { - ext.args = '--extension bedgraph' -} diff --git a/modules/nf-core/tidk/search/tests/tags.yml b/modules/nf-core/tidk/search/tests/tags.yml deleted file mode 100644 index 6d27fc6d..00000000 --- a/modules/nf-core/tidk/search/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -tidk/search: - - "modules/nf-core/tidk/search/**" diff --git a/modules/nf-core/untar/environment.yml b/modules/nf-core/untar/environment.yml deleted file mode 100644 index 0c9cbb10..00000000 --- a/modules/nf-core/untar/environment.yml +++ /dev/null @@ -1,11 +0,0 @@ -name: untar - -channels: - - conda-forge - - bioconda - - defaults - -dependencies: - - conda-forge::grep=3.11 - - conda-forge::sed=4.7 - - conda-forge::tar=1.34 diff --git a/modules/nf-core/untar/main.nf b/modules/nf-core/untar/main.nf deleted file mode 100644 index 250172e1..00000000 --- a/modules/nf-core/untar/main.nf +++ /dev/null @@ -1,62 +0,0 @@ -process UNTAR { - tag "$archive" - label 'process_single' - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : - 'quay.io/nf-core/ubuntu:20.04' }" - - input: - tuple val(meta), path(archive) - - output: - tuple val(meta), path("$prefix"), emit: untar - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def args2 = task.ext.args2 ?: '' - prefix = task.ext.prefix ?: ( meta.id ? "${meta.id}" : archive.baseName.toString().replaceFirst(/\.tar$/, "")) - - """ - mkdir $prefix - - ## Ensures --strip-components only applied when top level of tar contents is a directory - ## If just files or multiple directories, place all in prefix - if [[ \$(tar -taf ${archive} | grep -o -P "^.*?\\/" | uniq | wc -l) -eq 1 ]]; then - tar \\ - -C $prefix --strip-components 1 \\ - -xavf \\ - $args \\ - $archive \\ - $args2 - else - tar \\ - -C $prefix \\ - -xavf \\ - $args \\ - $archive \\ - $args2 - fi - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - untar: \$(echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//') - END_VERSIONS - """ - - stub: - prefix = task.ext.prefix ?: ( meta.id ? "${meta.id}" : archive.toString().replaceFirst(/\.[^\.]+(.gz)?$/, "")) - """ - mkdir $prefix - touch ${prefix}/file.txt - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - untar: \$(echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//') - END_VERSIONS - """ -} diff --git a/modules/nf-core/untar/meta.yml b/modules/nf-core/untar/meta.yml deleted file mode 100644 index a9a2110f..00000000 --- a/modules/nf-core/untar/meta.yml +++ /dev/null @@ -1,46 +0,0 @@ -name: untar -description: Extract files. -keywords: - - untar - - uncompress - - extract -tools: - - untar: - description: | - Extract tar.gz files. - documentation: https://www.gnu.org/software/tar/manual/ - licence: ["GPL-3.0-or-later"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - archive: - type: file - description: File to be untar - pattern: "*.{tar}.{gz}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - untar: - type: directory - description: Directory containing contents of archive - pattern: "*/" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@joseespinosa" - - "@drpatelh" - - "@matthdsm" - - "@jfy133" -maintainers: - - "@joseespinosa" - - "@drpatelh" - - "@matthdsm" - - "@jfy133" diff --git a/modules/nf-core/untar/tests/main.nf.test b/modules/nf-core/untar/tests/main.nf.test deleted file mode 100644 index 1106fa01..00000000 --- a/modules/nf-core/untar/tests/main.nf.test +++ /dev/null @@ -1,55 +0,0 @@ -nextflow_process { - - name "Test Process UNTAR" - script "../main.nf" - process "UNTAR" - - tag "modules" - tag "modules_nfcore" - tag "untar" - - test("test_untar") { - - when { - params { - outdir = "$outputDir" - } - process { - """ - input[0] = [ [], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/db/kraken2.tar.gz', checkIfExists: true) ] - """ - } - } - - then { - assertAll ( - { assert process.success }, - { assert snapshot(process.out.untar).match("test_untar") }, - ) - } - - } - - test("test_untar_onlyfiles") { - - when { - params { - outdir = "$outputDir" - } - process { - """ - input[0] = [ [], file(params.modules_testdata_base_path + 'generic/tar/hello.tar.gz', checkIfExists: true) ] - """ - } - } - - then { - assertAll ( - { assert process.success }, - { assert snapshot(process.out.untar).match("test_untar_onlyfiles") }, - ) - } - - } - -} \ No newline at end of file diff --git a/modules/nf-core/untar/tests/main.nf.test.snap b/modules/nf-core/untar/tests/main.nf.test.snap deleted file mode 100644 index ace42576..00000000 --- a/modules/nf-core/untar/tests/main.nf.test.snap +++ /dev/null @@ -1,34 +0,0 @@ -{ - "test_untar_onlyfiles": { - "content": [ - [ - [ - [ - - ], - [ - "hello.txt:md5,e59ff97941044f85df5297e1c302d260" - ] - ] - ] - ], - "timestamp": "2023-10-18T11:56:46.878844" - }, - "test_untar": { - "content": [ - [ - [ - [ - - ], - [ - "hash.k2d:md5,8b8598468f54a7087c203ad0190555d9", - "opts.k2d:md5,a033d00cf6759407010b21700938f543", - "taxo.k2d:md5,094d5891cdccf2f1468088855c214b2c" - ] - ] - ] - ], - "timestamp": "2023-10-18T11:56:08.16574" - } -} \ No newline at end of file diff --git a/modules/nf-core/untar/tests/tags.yml b/modules/nf-core/untar/tests/tags.yml deleted file mode 100644 index feb6f15c..00000000 --- a/modules/nf-core/untar/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -untar: - - modules/nf-core/untar/** diff --git a/modules/pfr/bwa/index/environment.yml b/modules/pfr/bwa/index/environment.yml deleted file mode 100644 index 5d3cb323..00000000 --- a/modules/pfr/bwa/index/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: bwa_index -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::bwa=0.7.17 diff --git a/modules/pfr/bwa/index/main.nf b/modules/pfr/bwa/index/main.nf deleted file mode 100644 index 3a3131ec..00000000 --- a/modules/pfr/bwa/index/main.nf +++ /dev/null @@ -1,52 +0,0 @@ -process BWA_INDEX { - tag "$fasta" - label 'process_single' - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/bwa:0.7.17--hed695b0_7' : - 'quay.io/biocontainers/bwa:0.7.17--hed695b0_7' }" - - input: - tuple val(meta), path(fasta) - - output: - tuple val(meta), path(bwa) , emit: index - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def prefix = task.ext.prefix ?: "${fasta.baseName}" - def args = task.ext.args ?: '' - """ - mkdir bwa - bwa \\ - index \\ - $args \\ - -p bwa/${prefix} \\ - $fasta - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bwa: \$(echo \$(bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//') - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${fasta.baseName}" - """ - mkdir bwa - - touch bwa/${prefix}.amb - touch bwa/${prefix}.ann - touch bwa/${prefix}.bwt - touch bwa/${prefix}.pac - touch bwa/${prefix}.sa - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bwa: \$(echo \$(bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//') - END_VERSIONS - """ -} diff --git a/modules/pfr/bwa/index/meta.yml b/modules/pfr/bwa/index/meta.yml deleted file mode 100644 index 730628d0..00000000 --- a/modules/pfr/bwa/index/meta.yml +++ /dev/null @@ -1,45 +0,0 @@ -name: bwa_index -description: Create BWA index for reference genome -keywords: - - index - - fasta - - genome - - reference -tools: - - bwa: - description: | - BWA is a software package for mapping DNA sequences against - a large reference genome, such as the human genome. - homepage: http://bio-bwa.sourceforge.net/ - documentation: http://www.htslib.org/doc/samtools.html - arxiv: arXiv:1303.3997 - licence: ["GPL-3.0-or-later"] -input: - - meta: - type: map - description: | - Groovy Map containing reference information. - e.g. [ id:'test', single_end:false ] - - fasta: - type: file - description: Input genome fasta file -output: - - meta: - type: map - description: | - Groovy Map containing reference information. - e.g. [ id:'test', single_end:false ] - - index: - type: file - description: BWA genome index files - pattern: "*.{amb,ann,bwt,pac,sa}" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@drpatelh" - - "@maxulysse" -maintainers: - - "@drpatelh" - - "@maxulysse" diff --git a/modules/pfr/bwa/index/tests/main.nf.test b/modules/pfr/bwa/index/tests/main.nf.test deleted file mode 100644 index 2a3a1608..00000000 --- a/modules/pfr/bwa/index/tests/main.nf.test +++ /dev/null @@ -1,33 +0,0 @@ -nextflow_process { - - name "Test Process BWA_INDEX" - tag "modules_nfcore" - tag "modules" - tag "bwa" - tag "bwa/index" - script "../main.nf" - process "BWA_INDEX" - - test("BWA index") { - - when { - process { - """ - input[0] = [ - [id: 'test'], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() } - ) - } - - } - -} \ No newline at end of file diff --git a/modules/pfr/bwa/index/tests/main.nf.test.snap b/modules/pfr/bwa/index/tests/main.nf.test.snap deleted file mode 100644 index e51ad5bf..00000000 --- a/modules/pfr/bwa/index/tests/main.nf.test.snap +++ /dev/null @@ -1,43 +0,0 @@ -{ - "BWA index": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - [ - "genome.amb:md5,3a68b8b2287e07dd3f5f95f4344ba76e", - "genome.ann:md5,c32e11f6c859f166c7525a9c1d583567", - "genome.bwt:md5,0469c30a1e239dd08f68afe66fde99da", - "genome.pac:md5,983e3d2cd6f36e2546e6d25a0da78d66", - "genome.sa:md5,ab3952cabf026b48cd3eb5bccbb636d1" - ] - ] - ], - "1": [ - "versions.yml:md5,0f20525da90e7489a7ebb02adca3265f" - ], - "index": [ - [ - { - "id": "test" - }, - [ - "genome.amb:md5,3a68b8b2287e07dd3f5f95f4344ba76e", - "genome.ann:md5,c32e11f6c859f166c7525a9c1d583567", - "genome.bwt:md5,0469c30a1e239dd08f68afe66fde99da", - "genome.pac:md5,983e3d2cd6f36e2546e6d25a0da78d66", - "genome.sa:md5,ab3952cabf026b48cd3eb5bccbb636d1" - ] - ] - ], - "versions": [ - "versions.yml:md5,0f20525da90e7489a7ebb02adca3265f" - ] - } - ], - "timestamp": "2023-10-17T17:20:20.180927714" - } -} \ No newline at end of file diff --git a/modules/pfr/bwa/index/tests/tags.yml b/modules/pfr/bwa/index/tests/tags.yml deleted file mode 100644 index 28bb483c..00000000 --- a/modules/pfr/bwa/index/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -bwa/index: - - modules/nf-core/bwa/index/** diff --git a/modules/pfr/bwa/mem/environment.yml b/modules/pfr/bwa/mem/environment.yml deleted file mode 100644 index 1818cea3..00000000 --- a/modules/pfr/bwa/mem/environment.yml +++ /dev/null @@ -1,10 +0,0 @@ -name: bwa_mem -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bwa=0.7.17 - # renovate: datasource=conda depName=bioconda/samtools - - samtools=1.18 - - htslib=1.18 diff --git a/modules/pfr/bwa/mem/main.nf b/modules/pfr/bwa/mem/main.nf deleted file mode 100644 index bc60783f..00000000 --- a/modules/pfr/bwa/mem/main.nf +++ /dev/null @@ -1,55 +0,0 @@ -process BWA_MEM { - tag "$meta.id" - label 'process_high' - label 'process_two_days_long' - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:9c0128851101dafef65cef649826d2dbe6bedd7e-0' : - 'quay.io/biocontainers/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:9c0128851101dafef65cef649826d2dbe6bedd7e-0' }" - - input: - tuple val(meta), path(reads) - tuple val(meta2), path(index) - val sort_bam - - output: - tuple val(meta), path("*.bam"), emit: bam - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def args2 = task.ext.args2 ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def samtools_command = sort_bam ? 'sort' : 'view' - """ - INDEX=`find -L ./ -name "*.amb" | sed 's/\\.amb\$//'` - - bwa mem \\ - $args \\ - -t $task.cpus \\ - \$INDEX \\ - $reads \\ - | samtools $samtools_command $args2 --threads $task.cpus -o ${prefix}.bam - - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bwa: \$(echo \$(bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//') - samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.bam - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bwa: \$(echo \$(bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//') - samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') - END_VERSIONS - """ -} diff --git a/modules/pfr/bwa/mem/meta.yml b/modules/pfr/bwa/mem/meta.yml deleted file mode 100644 index 440fb1f9..00000000 --- a/modules/pfr/bwa/mem/meta.yml +++ /dev/null @@ -1,58 +0,0 @@ -name: bwa_mem -description: Performs fastq alignment to a fasta reference using BWA -keywords: - - mem - - bwa - - alignment - - map - - fastq - - bam - - sam -tools: - - bwa: - description: | - BWA is a software package for mapping DNA sequences against - a large reference genome, such as the human genome. - homepage: http://bio-bwa.sourceforge.net/ - documentation: http://www.htslib.org/doc/samtools.html - arxiv: arXiv:1303.3997 - licence: ["GPL-3.0-or-later"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: | - List of input FastQ files of size 1 and 2 for single-end and paired-end data, - respectively. - - meta2: - type: map - description: | - Groovy Map containing reference information. - e.g. [ id:'test', single_end:false ] - - index: - type: file - description: BWA genome index files - pattern: "Directory containing BWA index *.{amb,ann,bwt,pac,sa}" - - sort_bam: - type: boolean - description: use samtools sort (true) or samtools view (false) - pattern: "true or false" -output: - - bam: - type: file - description: Output BAM file containing read alignments - pattern: "*.{bam}" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@drpatelh" - - "@jeremy1805" -maintainers: - - "@drpatelh" - - "@jeremy1805" diff --git a/modules/pfr/bwa/mem/tests/main.nf.test b/modules/pfr/bwa/mem/tests/main.nf.test deleted file mode 100644 index 20cb7e95..00000000 --- a/modules/pfr/bwa/mem/tests/main.nf.test +++ /dev/null @@ -1,173 +0,0 @@ -nextflow_process { - - name "Test Process BWA_MEM" - tag "modules_nfcore" - tag "modules" - tag "bwa" - tag "bwa/mem" - tag "bwa/index" - script "../main.nf" - process "BWA_MEM" - - test("Single-End") { - - setup { - run("BWA_INDEX") { - script "../../index/main.nf" - process { - """ - input[0] = [ - [id: 'test'], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) - ] - """ - } - } - } - - when { - process { - """ - input[0] = [ - [ id:'test', single_end:true ], // meta map - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] - ] - input[1] = BWA_INDEX.out.index - input[2] = false - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() } - ) - } - - } - - test("Single-End Sort") { - - setup { - run("BWA_INDEX") { - script "../../index/main.nf" - process { - """ - input[0] = [ - [id: 'test'], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) - ] - """ - } - } - } - - when { - process { - """ - input[0] = [ - [ id:'test', single_end:true ], // meta map - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] - ] - input[1] = BWA_INDEX.out.index - input[2] = true - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() } - ) - } - - } - - test("Paired-End") { - - setup { - run("BWA_INDEX") { - script "../../index/main.nf" - process { - """ - input[0] = [ - [id: 'test'], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) - ] - """ - } - } - } - - when { - process { - """ - input[0] = [ - [ id:'test', single_end:false ], // meta map - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) - ] - ] - input[1] = BWA_INDEX.out.index - input[2] = false - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() } - ) - } - - } - - test("Paired-End Sort") { - - setup { - run("BWA_INDEX") { - script "../../index/main.nf" - process { - """ - input[0] = [ - [id: 'test'], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) - ] - """ - } - } - } - - when { - process { - """ - input[0] = [ - [ id:'test', single_end:false ], // meta map - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) - ] - ] - input[1] = BWA_INDEX.out.index - input[2] = true - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() } - ) - } - - } -} \ No newline at end of file diff --git a/modules/pfr/bwa/mem/tests/main.nf.test.snap b/modules/pfr/bwa/mem/tests/main.nf.test.snap deleted file mode 100644 index bfb55fc7..00000000 --- a/modules/pfr/bwa/mem/tests/main.nf.test.snap +++ /dev/null @@ -1,126 +0,0 @@ -{ - "Single-End": { - "content": [ - { - "0": [ - [ - { - "id": "test", - "single_end": true - }, - "test.bam:md5,df203d7c7e8fef351408a909570c7952" - ] - ], - "1": [ - "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" - ], - "bam": [ - [ - { - "id": "test", - "single_end": true - }, - "test.bam:md5,df203d7c7e8fef351408a909570c7952" - ] - ], - "versions": [ - "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" - ] - } - ], - "timestamp": "2023-12-04T11:01:22.483594641" - }, - "Single-End Sort": { - "content": [ - { - "0": [ - [ - { - "id": "test", - "single_end": true - }, - "test.bam:md5,8a52bd78fdcecb994c1f63897d5b431c" - ] - ], - "1": [ - "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" - ], - "bam": [ - [ - { - "id": "test", - "single_end": true - }, - "test.bam:md5,8a52bd78fdcecb994c1f63897d5b431c" - ] - ], - "versions": [ - "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" - ] - } - ], - "timestamp": "2023-12-04T11:01:30.180783483" - }, - "Paired-End": { - "content": [ - { - "0": [ - [ - { - "id": "test", - "single_end": false - }, - "test.bam:md5,9815aef9ec763a60c53c3879be2d73ae" - ] - ], - "1": [ - "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" - ], - "bam": [ - [ - { - "id": "test", - "single_end": false - }, - "test.bam:md5,9815aef9ec763a60c53c3879be2d73ae" - ] - ], - "versions": [ - "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" - ] - } - ], - "timestamp": "2023-12-04T11:01:38.761983007" - }, - "Paired-End Sort": { - "content": [ - { - "0": [ - [ - { - "id": "test", - "single_end": false - }, - "test.bam:md5,0f0cda73704c4f7ba08af482edcbbe88" - ] - ], - "1": [ - "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" - ], - "bam": [ - [ - { - "id": "test", - "single_end": false - }, - "test.bam:md5,0f0cda73704c4f7ba08af482edcbbe88" - ] - ], - "versions": [ - "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" - ] - } - ], - "timestamp": "2023-12-04T11:01:46.284587802" - } -} \ No newline at end of file diff --git a/modules/pfr/bwa/mem/tests/tags.yml b/modules/pfr/bwa/mem/tests/tags.yml deleted file mode 100644 index 82992d1f..00000000 --- a/modules/pfr/bwa/mem/tests/tags.yml +++ /dev/null @@ -1,3 +0,0 @@ -bwa/mem: - - modules/nf-core/bwa/index/** - - modules/nf-core/bwa/mem/** diff --git a/modules/pfr/cat/cat/environment.yml b/modules/pfr/cat/cat/environment.yml deleted file mode 100644 index 17a04ef2..00000000 --- a/modules/pfr/cat/cat/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: cat_cat -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - conda-forge::pigz=2.3.4 diff --git a/modules/pfr/cat/cat/main.nf b/modules/pfr/cat/cat/main.nf deleted file mode 100644 index 99ac1985..00000000 --- a/modules/pfr/cat/cat/main.nf +++ /dev/null @@ -1,69 +0,0 @@ -process CAT_CAT { - tag "$meta.id" - label 'process_low' - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/pigz:2.3.4': - 'quay.io/biocontainers/pigz:2.3.4' }" - - input: - tuple val(meta), path(files_in) - - output: - tuple val(meta), path("${prefix}"), emit: file_out - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def args2 = task.ext.args2 ?: '' - def file_list = files_in.collect { it.toString() } - - // | input | output | command1 | command2 | - // |-----------|------------|----------|----------| - // | gzipped | gzipped | cat | | - // | ungzipped | ungzipped | cat | | - // | gzipped | ungzipped | zcat | | - // | ungzipped | gzipped | cat | pigz | - - // Use input file ending as default - prefix = task.ext.prefix ?: "${meta.id}${file_list[0].substring(file_list[0].lastIndexOf('.'))}" - out_zip = prefix.endsWith('.gz') - in_zip = file_list[0].endsWith('.gz') - command1 = (in_zip && !out_zip) ? 'zcat' : 'cat' - command2 = (!in_zip && out_zip) ? "| pigz -c -p $task.cpus $args2" : '' - if(file_list.contains(prefix.trim())) { - error "The name of the input file can't be the same as for the output prefix in the " + - "module CAT_CAT (currently `$prefix`). Please choose a different one." - } - """ - $command1 \\ - $args \\ - ${file_list.join(' ')} \\ - $command2 \\ - > ${prefix} - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' ) - END_VERSIONS - """ - - stub: - def file_list = files_in.collect { it.toString() } - prefix = task.ext.prefix ?: "${meta.id}${file_list[0].substring(file_list[0].lastIndexOf('.'))}" - if(file_list.contains(prefix.trim())) { - error "The name of the input file can't be the same as for the output prefix in the " + - "module CAT_CAT (currently `$prefix`). Please choose a different one." - } - """ - touch $prefix - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' ) - END_VERSIONS - """ -} diff --git a/modules/pfr/cat/cat/meta.yml b/modules/pfr/cat/cat/meta.yml deleted file mode 100644 index 00a8db0b..00000000 --- a/modules/pfr/cat/cat/meta.yml +++ /dev/null @@ -1,36 +0,0 @@ -name: cat_cat -description: A module for concatenation of gzipped or uncompressed files -keywords: - - concatenate - - gzip - - cat -tools: - - cat: - description: Just concatenation - documentation: https://man7.org/linux/man-pages/man1/cat.1.html - licence: ["GPL-3.0-or-later"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - files_in: - type: file - description: List of compressed / uncompressed files - pattern: "*" -output: - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - - file_out: - type: file - description: Concatenated file. Will be gzipped if file_out ends with ".gz" - pattern: "${file_out}" -authors: - - "@erikrikarddaniel" - - "@FriederikeHanssen" -maintainers: - - "@erikrikarddaniel" - - "@FriederikeHanssen" diff --git a/modules/pfr/cat/cat/tests/main.nf.test b/modules/pfr/cat/cat/tests/main.nf.test deleted file mode 100644 index b4056a81..00000000 --- a/modules/pfr/cat/cat/tests/main.nf.test +++ /dev/null @@ -1,178 +0,0 @@ -nextflow_process { - - name "Test Process CAT_CAT" - script "../main.nf" - process "CAT_CAT" - tag "modules" - tag "modules_nfcore" - tag "cat" - tag "cat/cat" - - test("test_cat_name_conflict") { - when { - params { - outdir = "${outputDir}" - } - process { - """ - input[0] = - [ - [ id:'genome', single_end:true ], - [ - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), - file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true) - ] - ] - """ - } - } - then { - assertAll( - { assert !process.success }, - { assert process.stdout.toString().contains("The name of the input file can't be the same as for the output prefix") } - ) - } - } - - test("test_cat_unzipped_unzipped") { - when { - params { - outdir = "${outputDir}" - } - process { - """ - input[0] = - [ - [ id:'test', single_end:true ], - [ - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), - file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true) - ] - ] - """ - } - } - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() } - ) - } - } - - - test("test_cat_zipped_zipped") { - when { - params { - outdir = "${outputDir}" - } - process { - """ - input[0] = - [ - [ id:'test', single_end:true ], - [ - file(params.test_data['sarscov2']['genome']['genome_gff3_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['genome']['contigs_genome_maf_gz'], checkIfExists: true) - ] - ] - """ - } - } - then { - def lines = path(process.out.file_out.get(0).get(1)).linesGzip - assertAll( - { assert process.success }, - { assert snapshot(lines[0..5]).match("test_cat_zipped_zipped_lines") }, - { assert snapshot(lines.size()).match("test_cat_zipped_zipped_size")} - ) - } - } - - test("test_cat_zipped_unzipped") { - config './nextflow_zipped_unzipped.config' - - when { - params { - outdir = "${outputDir}" - } - process { - """ - input[0] = - [ - [ id:'test', single_end:true ], - [ - file(params.test_data['sarscov2']['genome']['genome_gff3_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['genome']['contigs_genome_maf_gz'], checkIfExists: true) - ] - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() } - ) - } - - } - - test("test_cat_unzipped_zipped") { - config './nextflow_unzipped_zipped.config' - when { - params { - outdir = "${outputDir}" - } - process { - """ - input[0] = - [ - [ id:'test', single_end:true ], - [ - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), - file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true) - ] - ] - """ - } - } - then { - def lines = path(process.out.file_out.get(0).get(1)).linesGzip - assertAll( - { assert process.success }, - { assert snapshot(lines[0..5]).match("test_cat_unzipped_zipped_lines") }, - { assert snapshot(lines.size()).match("test_cat_unzipped_zipped_size")} - ) - } - } - - test("test_cat_one_file_unzipped_zipped") { - config './nextflow_unzipped_zipped.config' - when { - params { - outdir = "${outputDir}" - } - process { - """ - input[0] = - [ - [ id:'test', single_end:true ], - [ - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) - ] - ] - """ - } - } - then { - def lines = path(process.out.file_out.get(0).get(1)).linesGzip - assertAll( - { assert process.success }, - { assert snapshot(lines[0..5]).match("test_cat_one_file_unzipped_zipped_lines") }, - { assert snapshot(lines.size()).match("test_cat_one_file_unzipped_zipped_size")} - ) - } - } -} \ No newline at end of file diff --git a/modules/pfr/cat/cat/tests/main.nf.test.snap b/modules/pfr/cat/cat/tests/main.nf.test.snap deleted file mode 100644 index 423571ba..00000000 --- a/modules/pfr/cat/cat/tests/main.nf.test.snap +++ /dev/null @@ -1,121 +0,0 @@ -{ - "test_cat_unzipped_zipped_size": { - "content": [ - 375 - ], - "timestamp": "2023-10-16T14:33:08.049445686" - }, - "test_cat_unzipped_unzipped": { - "content": [ - { - "0": [ - [ - { - "id": "test", - "single_end": true - }, - "test.fasta:md5,f44b33a0e441ad58b2d3700270e2dbe2" - ] - ], - "1": [ - "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" - ], - "file_out": [ - [ - { - "id": "test", - "single_end": true - }, - "test.fasta:md5,f44b33a0e441ad58b2d3700270e2dbe2" - ] - ], - "versions": [ - "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" - ] - } - ], - "timestamp": "2023-10-16T14:32:18.500464399" - }, - "test_cat_zipped_unzipped": { - "content": [ - { - "0": [ - [ - { - "id": "test", - "single_end": true - }, - "cat.txt:md5,c439d3b60e7bc03e8802a451a0d9a5d9" - ] - ], - "1": [ - "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" - ], - "file_out": [ - [ - { - "id": "test", - "single_end": true - }, - "cat.txt:md5,c439d3b60e7bc03e8802a451a0d9a5d9" - ] - ], - "versions": [ - "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" - ] - } - ], - "timestamp": "2023-10-16T14:32:49.642741302" - }, - "test_cat_zipped_zipped_lines": { - "content": [ - [ - "MT192765.1\tGenbank\ttranscript\t259\t29667\t.\t+\t.\tID=unknown_transcript_1;geneID=orf1ab;gene_name=orf1ab", - "MT192765.1\tGenbank\tgene\t259\t21548\t.\t+\t.\tParent=unknown_transcript_1", - "MT192765.1\tGenbank\tCDS\t259\t13461\t.\t+\t0\tParent=unknown_transcript_1;exception=\"ribosomal slippage\";gbkey=CDS;gene=orf1ab;note=\"pp1ab;translated=by -1 ribosomal frameshift\";product=\"orf1ab polyprotein\";protein_id=QIK50426.1", - "MT192765.1\tGenbank\tCDS\t13461\t21548\t.\t+\t0\tParent=unknown_transcript_1;exception=\"ribosomal slippage\";gbkey=CDS;gene=orf1ab;note=\"pp1ab;translated=by -1 ribosomal frameshift\";product=\"orf1ab polyprotein\";protein_id=QIK50426.1", - "MT192765.1\tGenbank\tCDS\t21556\t25377\t.\t+\t0\tParent=unknown_transcript_1;gbkey=CDS;gene=S;note=\"structural protein\";product=\"surface glycoprotein\";protein_id=QIK50427.1", - "MT192765.1\tGenbank\tgene\t21556\t25377\t.\t+\t.\tParent=unknown_transcript_1" - ] - ], - "timestamp": "2023-10-16T14:32:33.629048645" - }, - "test_cat_unzipped_zipped_lines": { - "content": [ - [ - ">MT192765.1 Severe acute respiratory syndrome coronavirus 2 isolate SARS-CoV-2/human/USA/PC00101P/2020, complete genome", - "GTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGT", - "GTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAG", - "TAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGG", - "GTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTT", - "ACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAG" - ] - ], - "timestamp": "2023-10-16T14:33:08.038830506" - }, - "test_cat_one_file_unzipped_zipped_lines": { - "content": [ - [ - ">MT192765.1 Severe acute respiratory syndrome coronavirus 2 isolate SARS-CoV-2/human/USA/PC00101P/2020, complete genome", - "GTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGT", - "GTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAG", - "TAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGG", - "GTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTT", - "ACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAG" - ] - ], - "timestamp": "2023-10-16T14:33:21.39642399" - }, - "test_cat_zipped_zipped_size": { - "content": [ - 78 - ], - "timestamp": "2023-10-16T14:32:33.641869244" - }, - "test_cat_one_file_unzipped_zipped_size": { - "content": [ - 374 - ], - "timestamp": "2023-10-16T14:33:21.4094373" - } -} \ No newline at end of file diff --git a/modules/pfr/cat/cat/tests/nextflow_unzipped_zipped.config b/modules/pfr/cat/cat/tests/nextflow_unzipped_zipped.config deleted file mode 100644 index ec26b0fd..00000000 --- a/modules/pfr/cat/cat/tests/nextflow_unzipped_zipped.config +++ /dev/null @@ -1,6 +0,0 @@ - -process { - withName: CAT_CAT { - ext.prefix = 'cat.txt.gz' - } -} diff --git a/modules/pfr/cat/cat/tests/nextflow_zipped_unzipped.config b/modules/pfr/cat/cat/tests/nextflow_zipped_unzipped.config deleted file mode 100644 index fbc79783..00000000 --- a/modules/pfr/cat/cat/tests/nextflow_zipped_unzipped.config +++ /dev/null @@ -1,8 +0,0 @@ - -process { - - withName: CAT_CAT { - ext.prefix = 'cat.txt' - } - -} diff --git a/modules/pfr/cat/cat/tests/tags.yml b/modules/pfr/cat/cat/tests/tags.yml deleted file mode 100644 index 37b578f5..00000000 --- a/modules/pfr/cat/cat/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -cat/cat: - - modules/nf-core/cat/cat/** diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/environment.yml b/modules/pfr/custom/checkgff3fastacorrespondence/environment.yml deleted file mode 100644 index ec0e86d1..00000000 --- a/modules/pfr/custom/checkgff3fastacorrespondence/environment.yml +++ /dev/null @@ -1,9 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json -name: "custom_checkgff3fastacorrespondence" -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - "bioconda::samtools=1.18" diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/main.nf b/modules/pfr/custom/checkgff3fastacorrespondence/main.nf deleted file mode 100644 index 09b472c0..00000000 --- a/modules/pfr/custom/checkgff3fastacorrespondence/main.nf +++ /dev/null @@ -1,25 +0,0 @@ -process CUSTOM_CHECKGFF3FASTACORRESPONDENCE { - tag "$meta.id" - label 'process_single' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1': - 'quay.io/biocontainers/samtools:1.18--h50ea8bc_1' }" - - input: - tuple val(meta), path(gff3) - path(fasta) - - output: - tuple val(meta), path('*.success.log') , emit: success_log , optional: true - tuple val(meta), path('*.error.log') , emit: error_log , optional: true - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - shell: - prefix = task.ext.prefix ?: "${meta.id}" - template 'check_gff3_fasta_correspondence.sh' -} diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/meta.yml b/modules/pfr/custom/checkgff3fastacorrespondence/meta.yml deleted file mode 100644 index 69bbd053..00000000 --- a/modules/pfr/custom/checkgff3fastacorrespondence/meta.yml +++ /dev/null @@ -1,56 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: "custom_checkgff3fastacorrespondence" -description: "A custom bash script which checks the correspondence of a gff3 file with a fasta file" -keywords: - - genome - - gff3 - - annotation - - validation -tools: - - samtools: - description: | - SAMtools is a set of utilities for interacting with and post-processing - short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. - These files are generated as output by short read aligners like BWA. - homepage: http://www.htslib.org/ - documentation: http://www.htslib.org/doc/samtools.html - doi: 10.1093/bioinformatics/btp352 - licence: ["MIT"] - -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test' ]` - - gff3: - type: file - description: Input gff3 file - pattern: "*.{gff,gff3}" - - fasta: - type: file - description: Input fasta file - pattern: "*.{fsa,fa,fasta}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test' ]` - - success_log: - type: file - description: Log file for successful validation - pattern: "*.success.log" - - error_log: - type: file - description: Log file for failed validation - pattern: "*.error.log" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@GallVp" -maintainers: - - "@GallVp" diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/templates/check_gff3_fasta_correspondence.sh b/modules/pfr/custom/checkgff3fastacorrespondence/templates/check_gff3_fasta_correspondence.sh deleted file mode 100755 index 611c64b3..00000000 --- a/modules/pfr/custom/checkgff3fastacorrespondence/templates/check_gff3_fasta_correspondence.sh +++ /dev/null @@ -1,70 +0,0 @@ -#!/usr/bin/env bash - -# Bump VERSION on edit -VERSION="v1" - -gff3_file="!{gff3}" -fasta_file="!{fasta}" -out_prefix="!{prefix}" -task_process="!{task.process}" - -# Record versions -cat <<-END_VERSIONS > versions.yml -"${task_process}": - samtools: $(echo $(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*$//' ) -END_VERSIONS - -# Requires -# samtools faidx - -## STEP 1 -# Check that gff3 has no identifers that are not in fasta (fasta can -# have ids that are not in gff3 since not all assembly units have gff3 records - -# Extract identifiers from the GFF3 file -gff3_identifiers=$(grep -v '^#' "$gff3_file" | awk '{print $1}' | sort -u) - -# Extract identifiers from the FASTA file -fasta_identifiers=$(grep '^>' "$fasta_file" | awk '{print substr($1, 2)}' | sort -u) - -# Compare identifiers and find any that are present in the GFF3 but not in the FASTA -missing_identifiers=$(comm -23 <(echo "$gff3_identifiers") <(echo "$fasta_identifiers")) - -# Check if any missing identifiers were found -if [[ -n "$missing_identifiers" ]]; then - touch "${out_prefix}.error.log" - echo "Failed to validate gff3 file for: $tag_label" >> "${out_prefix}.error.log" - echo "Fasta file: $fasta_file" >> "${out_prefix}.error.log" - echo "Gff3 file: $gff3_file" >> "${out_prefix}.error.log" - echo "GFF3 file contains identifiers not present in FASTA:" >> "${out_prefix}.error.log" - echo "$missing_identifiers" >> "${out_prefix}.error.log" - exit 0 -fi - -## STEP 2 -# check that there are no coordiantes in gff3 for any seqid that are -# greater than the seq length of the paretn fasta entry - -# Compute sequence lengths using samtools faidx -samtools faidx "$fasta_file" | cut -f 1,2 > sequence_lengths.txt - -# Check GFF3 file for coordinates exceeding sequence lengths -while IFS=$'\t' read -r seqname source feature start end score strand frame attributes && \ - read -r seq seq_length <&3; do - if [[ $start -gt $seq_length || $end -gt $seq_length ]]; then - touch "${out_prefix}.error.log" - echo "Failed to validate gff3 file for: $tag_label" >> "${out_prefix}.error.log" - echo "Fasta file: $fasta_file" >> "${out_prefix}.error.log" - echo "Gff3 file: $gff3_file" >> "${out_prefix}.error.log" - echo "Coordinates exceed sequence length in GFF3 file:" >> "${out_prefix}.error.log" - echo "Sequence: $seqname" >> "${out_prefix}.error.log" - echo "Sequence length: $seq_length" >> "${out_prefix}.error.log" - echo "Start: $start" >> "${out_prefix}.error.log" - echo "End: $end" >> "${out_prefix}.error.log" - exit 0 - fi -done < "$gff3_file" 3< "sequence_lengths.txt" - -touch "${out_prefix}.success.log" -echo "All tests passed..." >> "${out_prefix}.success.log" -exit 0 diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test b/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test deleted file mode 100644 index a45992c2..00000000 --- a/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test +++ /dev/null @@ -1,70 +0,0 @@ -nextflow_process { - - name "Test Process CUSTOM_CHECKGFF3FASTACORRESPONDENCE" - script "../main.nf" - process "CUSTOM_CHECKGFF3FASTACORRESPONDENCE" - - tag "modules" - tag "modules_nfcore" - tag "custom" - tag "custom/checkgff3fastacorrespondence" - - test("sarscov2-fasta-gff3-success") { - - when { - process { - """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) - ] - - input[1] = [ - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert process.out.error_log == [] }, - { assert process.out.success_log != null }, - { assert path(process.out.success_log.get(0).get(1)).getText().contains("All tests passed...")}, - ) - } - - } - - test("sarscov2-gff3-homo_sapiens-fasta-error") { - - when { - process { - """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) - ] - - input[1] = [ - file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert process.out.success_log == [] }, - { assert process.out.error_log != null }, - { assert path(process.out.error_log.get(0).get(1)).getText().contains("GFF3 file contains identifiers not present in FASTA")}, - ) - } - - } - -} \ No newline at end of file diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test.snap b/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test.snap deleted file mode 100644 index 261e0dc3..00000000 --- a/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test.snap +++ /dev/null @@ -1,72 +0,0 @@ -{ - "sarscov2-gff3-homo_sapiens-fasta-error": { - "content": [ - { - "0": [ - - ], - "1": [ - [ - { - "id": "test" - }, - "test.error.log:md5,8a119170625dc95fb2faa6843fad2c3f" - ] - ], - "2": [ - "versions.yml:md5,c8e0bb60f7422aa6c15db35013620802" - ], - "error_log": [ - [ - { - "id": "test" - }, - "test.error.log:md5,8a119170625dc95fb2faa6843fad2c3f" - ] - ], - "success_log": [ - - ], - "versions": [ - "versions.yml:md5,c8e0bb60f7422aa6c15db35013620802" - ] - } - ], - "timestamp": "2023-11-29T12:24:08.677505" - }, - "sarscov2-fasta-gff3-success": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.success.log:md5,5cad27984e6af4889f7dcf12264fe47b" - ] - ], - "1": [ - - ], - "2": [ - "versions.yml:md5,c8e0bb60f7422aa6c15db35013620802" - ], - "error_log": [ - - ], - "success_log": [ - [ - { - "id": "test" - }, - "test.success.log:md5,5cad27984e6af4889f7dcf12264fe47b" - ] - ], - "versions": [ - "versions.yml:md5,c8e0bb60f7422aa6c15db35013620802" - ] - } - ], - "timestamp": "2023-11-29T12:24:04.530428" - } -} \ No newline at end of file diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/tests/tags.yml b/modules/pfr/custom/checkgff3fastacorrespondence/tests/tags.yml deleted file mode 100644 index 708130d8..00000000 --- a/modules/pfr/custom/checkgff3fastacorrespondence/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -custom/checkgff3fastacorrespondence: - - "modules/pfr/custom/checkgff3fastacorrespondence/**" diff --git a/modules/pfr/custom/restoregffids/environment.yml b/modules/pfr/custom/restoregffids/environment.yml deleted file mode 100644 index 2450c456..00000000 --- a/modules/pfr/custom/restoregffids/environment.yml +++ /dev/null @@ -1,9 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json -name: "custom_restoregffids" -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - "python=3.10.2" diff --git a/modules/pfr/custom/restoregffids/main.nf b/modules/pfr/custom/restoregffids/main.nf deleted file mode 100644 index b215f439..00000000 --- a/modules/pfr/custom/restoregffids/main.nf +++ /dev/null @@ -1,34 +0,0 @@ -process CUSTOM_RESTOREGFFIDS { - tag "$meta.id" - label 'process_single' - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/python:3.10.2': - 'quay.io/biocontainers/python:3.10.2' }" - - input: - tuple val(meta), path(gff3) - path(ids_tsv) - - output: - tuple val(meta), path("*.restored.ids.gff3") , emit: restored_ids_gff3 - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - prefix = task.ext.prefix ?: "${meta.id}" - template 'restore_gff_ids.py' - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch "${prefix}.restored.ids.gff3" - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - python: \$(python --version | cut -d' ' -f2) - END_VERSIONS - """ -} diff --git a/modules/pfr/custom/restoregffids/meta.yml b/modules/pfr/custom/restoregffids/meta.yml deleted file mode 100644 index 4e42b829..00000000 --- a/modules/pfr/custom/restoregffids/meta.yml +++ /dev/null @@ -1,58 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: "custom_restoregffids" -description: | - Restores IDs in a gff3 file based on a TSV table - consisting of original (first column) and new IDs (second column). - This module is helpful when some tools like EDTA implicitly shorten - the IDs without producing the ID map, leading to downstream mismatch - in IDs across files. -keywords: - - genome - - gff - - ID - - shorten - - restore -tools: - - "python": - description: | - Python is a programming language that lets you work quickly - and integrate systems more effectively - homepage: "https://www.python.org" - documentation: "https://docs.python.org/3/" - tool_dev_url: "https://github.com/python/cpython" - licence: ["MIT"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test' ]` - - gff3: - type: file - description: Input gff3 file - pattern: "*.{gff,gff3}" - - ids_tsv: - type: file - description: | - A TSV file with original (first column) and new ids (second column) - if id change was required - pattern: "*.tsv" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test' ]` - - restored_ids_gff3: - type: file - description: GFF3 file with restored ids - pattern: "*.restored.ids.gff3" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@GallVp" -maintainers: - - "@GallVp" diff --git a/modules/pfr/custom/restoregffids/templates/restore_gff_ids.py b/modules/pfr/custom/restoregffids/templates/restore_gff_ids.py deleted file mode 100755 index d0699de0..00000000 --- a/modules/pfr/custom/restoregffids/templates/restore_gff_ids.py +++ /dev/null @@ -1,47 +0,0 @@ -#!/usr/bin/env python3 - -from platform import python_version - -ids_tsv = "$ids_tsv" -input_gff3 = "$gff3" -output_prefix = "$prefix" - - -def create_name_mapping_from_tsv(file_path): - dictionary = {} - - with open(file_path, "r") as tsv_file: - for line in tsv_file: - columns = line.strip().split("\\t") - if len(columns) != 2: - raise ValueError(f"{file_path} should be a two column TSV file") - - orig_id, new_id = columns[0], columns[1] - dictionary[new_id] = orig_id - - return dictionary - - -def restore_gff3_ids(new_to_orig_ids, file_path, output_file_name): - # Write versions - with open(f"versions.yml", "w") as f_versions: - f_versions.write('"${task.process}":\\n') - f_versions.write(f" python: {python_version()}\\n") - - with open(file_path, "r") as input_gff3_file: - input_lines = input_gff3_file.readlines() - - with open(output_file_name, "w") as output_gff_file: - for line in input_lines: - if line.startswith("##"): - output_gff_file.write(line) - continue - - new_id = line.split("\\t")[0] - orig_id = new_to_orig_ids[new_id] - output_gff_file.write("\\t".join([orig_id] + line.split("\\t")[1:])) - - -if __name__ == "__main__": - new_to_orig_ids = create_name_mapping_from_tsv(ids_tsv) - restore_gff3_ids(new_to_orig_ids, input_gff3, f"{output_prefix}.restored.ids.gff3") diff --git a/modules/pfr/custom/restoregffids/tests/main.nf.test b/modules/pfr/custom/restoregffids/tests/main.nf.test deleted file mode 100644 index cc374b76..00000000 --- a/modules/pfr/custom/restoregffids/tests/main.nf.test +++ /dev/null @@ -1,63 +0,0 @@ -nextflow_process { - - name "Test Process CUSTOM_RESTOREGFFIDS" - script "../main.nf" - process "CUSTOM_RESTOREGFFIDS" - - tag "modules" - tag "modules_nfcore" - tag "custom" - tag "custom/restoregffids" - - test("sarscov2-genome_gff3-success") { - when { - process { - """ - input[0] = [ - [ id:'test', single_end:false ], // meta map - file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) - ] - input[1] = Channel.of('Chr1\tMT192765.1').collectFile(name: 'id_map.tsv', newLine: true) - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert path(process.out.restored_ids_gff3.get(0).get(1)).getText().contains("Chr1") }, - { assert !path(process.out.restored_ids_gff3.get(0).get(1)).getText().contains("MT192765.1") }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - - test("stub") { - - options '-stub' - - when { - process { - """ - input[0] = [ - [ id:'test', single_end:false ], // meta map - file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) - ] - input[1] = Channel.of('Chr1\tMT192765.1').collectFile(name: 'id_map.tsv', newLine: true) - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert process.out.restored_ids_gff3 != null }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - -} \ No newline at end of file diff --git a/modules/pfr/custom/restoregffids/tests/main.nf.test.snap b/modules/pfr/custom/restoregffids/tests/main.nf.test.snap deleted file mode 100644 index ffe43e74..00000000 --- a/modules/pfr/custom/restoregffids/tests/main.nf.test.snap +++ /dev/null @@ -1,41 +0,0 @@ -{ - "sarscov2-genome_gff3-success": { - "content": [ - { - "0": [ - [ - { - "id": "test", - "single_end": false - }, - "test.restored.ids.gff3:md5,2c294938b9eb4e52d19e14725c1d92a9" - ] - ], - "1": [ - "versions.yml:md5,32d31c4f1da9a3d1be013fd163e5867e" - ], - "restored_ids_gff3": [ - [ - { - "id": "test", - "single_end": false - }, - "test.restored.ids.gff3:md5,2c294938b9eb4e52d19e14725c1d92a9" - ] - ], - "versions": [ - "versions.yml:md5,32d31c4f1da9a3d1be013fd163e5867e" - ] - } - ], - "timestamp": "2023-12-07T13:49:30.047425" - }, - "versions": { - "content": [ - [ - "versions.yml:md5,32d31c4f1da9a3d1be013fd163e5867e" - ] - ], - "timestamp": "2023-12-07T13:49:30.071175" - } -} \ No newline at end of file diff --git a/modules/pfr/custom/restoregffids/tests/tags.yml b/modules/pfr/custom/restoregffids/tests/tags.yml deleted file mode 100644 index 1d4b9a83..00000000 --- a/modules/pfr/custom/restoregffids/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -custom/restoregffids: - - "modules/pfr/custom/restoregffids/**" diff --git a/modules/pfr/custom/shortenfastaids/environment.yml b/modules/pfr/custom/shortenfastaids/environment.yml deleted file mode 100644 index e80fa7cf..00000000 --- a/modules/pfr/custom/shortenfastaids/environment.yml +++ /dev/null @@ -1,11 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json -name: "custom_shortenfastaids" -channels: - - conda-forge - - bioconda - - defaults - -dependencies: - - biopython==1.75 - - python=3.8 diff --git a/modules/pfr/custom/shortenfastaids/main.nf b/modules/pfr/custom/shortenfastaids/main.nf deleted file mode 100644 index f268d777..00000000 --- a/modules/pfr/custom/shortenfastaids/main.nf +++ /dev/null @@ -1,33 +0,0 @@ -process CUSTOM_SHORTENFASTAIDS { - tag "$meta.id" - label 'process_single' - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/biopython:1.75': - 'quay.io/biocontainers/biopython:1.75' }" - - input: - tuple val(meta), path(fasta) - - output: - tuple val(meta), path("*.short.ids.fasta") , emit: short_ids_fasta , optional: true - tuple val(meta), path("*.short.ids.tsv") , emit: short_ids_tsv , optional: true - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - prefix = task.ext.prefix ?: "${meta.id}" - template 'shorten_fasta_ids.py' - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - cat <<-END_VERSIONS > versions.yml - "${task.process}": - python: \$(python --version | cut -d' ' -f2) - biopython: \$(pip list | grep "biopython" | cut -d' ' -f3) - END_VERSIONS - """ -} diff --git a/modules/pfr/custom/shortenfastaids/meta.yml b/modules/pfr/custom/shortenfastaids/meta.yml deleted file mode 100644 index 2425810d..00000000 --- a/modules/pfr/custom/shortenfastaids/meta.yml +++ /dev/null @@ -1,58 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: "custom_shortenfastaids" -description: | - Shortens fasta IDs and produces a new fasta along with a TSV table - consisting of original (first column) and new IDs (second column). - This module is helpful when some tools like EDTA implicitly shorten - the IDs without producing the ID map, leading to downstream mismatch - in IDs across files. -keywords: - - genome - - fasta - - ID - - shorten -tools: - - "biopython": - description: | - Biopython is a set of freely available tools for biological computation written in Python by - an international team of developers. - homepage: "https://biopython.org" - documentation: "https://biopython.org/wiki/Documentation" - tool_dev_url: "https://github.com/biopython/biopython" - doi: "10.1093/bioinformatics/btp163" - licence: ["MIT"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test' ]` - - fasta: - type: file - description: Input fasta file - pattern: "*.{fsa,fa,fasta}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test' ]` - - short_ids_fasta: - type: file - description: Fasta file with shortened ids if id change is required - pattern: "*.{fsa,fa,fasta}" - - short_ids_tsv: - type: file - description: | - A TSV file with original (first column) and new ids (second column) - if id change is required - pattern: "*.tsv" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@GallVp" -maintainers: - - "@GallVp" diff --git a/modules/pfr/custom/shortenfastaids/templates/shorten_fasta_ids.py b/modules/pfr/custom/shortenfastaids/templates/shorten_fasta_ids.py deleted file mode 100755 index 54f35bf6..00000000 --- a/modules/pfr/custom/shortenfastaids/templates/shorten_fasta_ids.py +++ /dev/null @@ -1,181 +0,0 @@ -#!/usr/bin/env python3 - -import re - -from Bio import SeqIO -from importlib.metadata import version -from platform import python_version - -# The input fasta file path -fasta_file_path = "$fasta" -output_files_prefix = "$prefix" - - -def extract_fasta_ids_and_descriptions(fasta_file_path): - fasta_file_obj = SeqIO.parse(fasta_file_path, "fasta") - - ids = [] - for record in fasta_file_obj: - ids.append((record.id, record.description)) - return ids - - -def write_fasta_with_new_ids(fasta_file_path, id_mapping, file_prefix): - old_fasta_file_obj = SeqIO.parse(fasta_file_path, "fasta") - id_map = dict(id_mapping) - - replaced_records = [] - for record in old_fasta_file_obj: - old_id = record.id - - new_id = id_map[old_id] - record.id = new_id - record.description = "" - - replaced_records.append(record) - - SeqIO.write(replaced_records, f"{file_prefix}.short.ids.fasta", "fasta") - - -def do_id_need_to_change(id_and_description, silent=False): - id = id_and_description[0] - description = id_and_description[1] - if len(id) > 13: - if not silent: - print(f"{id} has length greater than 13") - return True - - if not re.match(r"^[a-zA-Z0-9_]+\$", id): - if not silent: - print(f"{id} does not match '^[a-zA-Z0-9_]+\$'") - return True - - if description != id and description != "": - if not silent: - print(f"{id} contains a comment: {description.replace(id, '')}") - return True - - if not silent: - print(f"{id} is acceptable") - return False - - -def do_ids_need_to_change(ids_and_descriptions, silent=False): - return any( - [ - do_id_need_to_change(id_and_description, silent) - for id_and_description in ids_and_descriptions - ] - ) - - -def extract_common_patterns(ids): - pattern_counts = {} - for id in ids: - patterns = re.findall(r"[A-Za-z0_]{4,}", id) - for pattern in set(patterns): - pattern_counts[pattern] = pattern_counts.get(pattern, 0) + 1 - - common_patterns = [ - pattern for pattern, count in pattern_counts.items() if count >= 2 - ] - - if len(common_patterns) < 1: - return {} - - return {pattern: pattern[:3] for pattern in common_patterns} - - -def shorten_ids(input_ids_and_descriptions, patterns_dict): - shortened_ids = [] - - for id_and_description in input_ids_and_descriptions: - id = id_and_description[0] - description = "" # Treat description as absent as it will be removed by write_fasta_with_new_ids - if not do_id_need_to_change((id, description), silent=True): - shortened_ids.append(id) - continue - - shortened_id = shorten_id_by_pattern_replacement(patterns_dict, id) - - if not do_id_need_to_change((shortened_id, description), silent=True): - shortened_ids.append(shortened_id) - continue - - shortened_id = f"Ctg{generate_hash(id)}" - - if not do_id_need_to_change((shortened_id, description), silent=True): - shortened_ids.append(shortened_id) - continue - - raise ValueError(f"Failed to shorten id: {id} ({shortened_id})") - - return shortened_ids - - -def shorten_id_by_pattern_replacement(patterns_dict, id): - if patterns_dict == {}: - return id - - shortened_id = id - matches_for_id = match_substrings(patterns_dict.keys(), shortened_id) - - for pattern in matches_for_id: - shortened_id = re.sub( - r"({})".format(re.escape(pattern)), - patterns_dict[pattern], - shortened_id, - ) - return ( - shortened_id - if shortened_id[len(shortened_id) - 1] != "_" - else shortened_id[0 : (len(shortened_id) - 1)] - ) - - -def match_substrings(substrings, target_string): - pattern = "|".join(map(re.escape, substrings)) - matches = re.findall(pattern, target_string) - return matches - - -def generate_hash(string): - import hashlib - - hash_object = hashlib.sha1(string.encode()) - full_hash = hash_object.hexdigest() - short_hash = full_hash[:10] - return short_hash - - -def fail_if_new_ids_not_valid(ids): - if len(ids) != len(set(ids)): - raise ValueError("Th new IDs are not unique") - - -if __name__ == "__main__": - input_ids_and_descriptions = extract_fasta_ids_and_descriptions(fasta_file_path) - input_ids = [x[0] for x in input_ids_and_descriptions] - - # Write versions - with open(f"versions.yml", "w") as f_versions: - f_versions.write('"${task.process}":\\n') - f_versions.write(f" python: {python_version()}\\n") - f_versions.write(f" biopython: {version('biopython')}\\n") - - if not do_ids_need_to_change(input_ids_and_descriptions): - print("IDs have acceptable length and character. No change required.") - exit(0) - - new_ids = shorten_ids( - input_ids_and_descriptions, extract_common_patterns(input_ids) - ) - fail_if_new_ids_not_valid(new_ids) - - with open(f"{output_files_prefix}.short.ids.tsv", "w") as f: - for input_id, new_id in zip(input_ids, new_ids): - f.write(f"{input_id}\\t{new_id}\\n") - - write_fasta_with_new_ids( - fasta_file_path, zip(input_ids, new_ids), output_files_prefix - ) diff --git a/modules/pfr/custom/shortenfastaids/tests/main.nf.test b/modules/pfr/custom/shortenfastaids/tests/main.nf.test deleted file mode 100644 index efff639e..00000000 --- a/modules/pfr/custom/shortenfastaids/tests/main.nf.test +++ /dev/null @@ -1,131 +0,0 @@ -nextflow_process { - - name "Test Process CUSTOM_SHORTENFASTAIDS" - script "../main.nf" - process "CUSTOM_SHORTENFASTAIDS" - - tag "modules" - tag "modules_nfcore" - tag "custom" - tag "custom/shortenfastaids" - - test("homo_sapiens-genome_fasta-no_change") { - - when { - process { - """ - input[0] = [ - [ id:'test' ], - file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert snapshot(process.out.versions).match("versions") }, - { assert process.out.short_ids_fasta == [] }, - { assert process.out.short_ids_tsv == [] } - ) - } - - } - - test("sarscov2-genome_fasta-pattern_change") { - - when { - process { - """ - input[0] = [ - [ id:'test' ], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - - test("homo_sapiens-genome2_fasta-length_change") { - - when { - process { - """ - input[0] = [ - [ id:'test' ], - file(params.test_data['homo_sapiens']['genome']['genome2_fasta'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - - test("custom_fasta-comment_change") { - - when { - process { - """ - input[0] = Channel.of('>Chr1 This is a test comment', 'AGCTAGCT') - | collectFile(name: 'sample.fasta', newLine: true) - | map { file -> [ [ id:'test' ], file ] } - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - - test("stub") { - - options "-stub" - - when { - process { - """ - input[0] = [ - [ id:'test' ], - file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out.versions).match("versions") }, - { assert process.out.short_ids_fasta == [] }, - { assert process.out.short_ids_tsv == [] } - ) - } - - } - -} \ No newline at end of file diff --git a/modules/pfr/custom/shortenfastaids/tests/main.nf.test.snap b/modules/pfr/custom/shortenfastaids/tests/main.nf.test.snap deleted file mode 100644 index 8fed1b9d..00000000 --- a/modules/pfr/custom/shortenfastaids/tests/main.nf.test.snap +++ /dev/null @@ -1,170 +0,0 @@ -{ - "custom_fasta-comment_change": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.short.ids.fasta:md5,c861b9d46a4d9bdba66953cff572fc5d" - ] - ], - "1": [ - [ - { - "id": "test" - }, - "test.short.ids.tsv:md5,8762f2bffbdff75c2812bad72ba52bba" - ] - ], - "2": [ - "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba" - ], - "short_ids_fasta": [ - [ - { - "id": "test" - }, - "test.short.ids.fasta:md5,c861b9d46a4d9bdba66953cff572fc5d" - ] - ], - "short_ids_tsv": [ - [ - { - "id": "test" - }, - "test.short.ids.tsv:md5,8762f2bffbdff75c2812bad72ba52bba" - ] - ], - "versions": [ - "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba" - ] - } - ], - "timestamp": "2023-12-07T13:33:05.523745" - }, - "versions": { - "content": [ - [ - "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba" - ] - ], - "timestamp": "2023-12-07T13:30:30.361527" - }, - "homo_sapiens-genome_fasta-no_change": { - "content": [ - { - "0": [ - - ], - "1": [ - - ], - "2": [ - "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba" - ], - "short_ids_fasta": [ - - ], - "short_ids_tsv": [ - - ], - "versions": [ - "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba" - ] - } - ], - "timestamp": "2023-12-07T13:32:54.220188" - }, - "homo_sapiens-genome2_fasta-length_change": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.short.ids.fasta:md5,1382acd98d4cd233a8062ef01b2aaa6d" - ] - ], - "1": [ - [ - { - "id": "test" - }, - "test.short.ids.tsv:md5,99c0f2a529cb595b2d8530024ed2880e" - ] - ], - "2": [ - "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba" - ], - "short_ids_fasta": [ - [ - { - "id": "test" - }, - "test.short.ids.fasta:md5,1382acd98d4cd233a8062ef01b2aaa6d" - ] - ], - "short_ids_tsv": [ - [ - { - "id": "test" - }, - "test.short.ids.tsv:md5,99c0f2a529cb595b2d8530024ed2880e" - ] - ], - "versions": [ - "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba" - ] - } - ], - "timestamp": "2023-12-07T13:33:01.924483" - }, - "sarscov2-genome_fasta-pattern_change": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.short.ids.fasta:md5,14d6f587b6d28889c5c0f985e78d602f" - ] - ], - "1": [ - [ - { - "id": "test" - }, - "test.short.ids.tsv:md5,d7a2af88e8549586e5616bff6a88bd71" - ] - ], - "2": [ - "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba" - ], - "short_ids_fasta": [ - [ - { - "id": "test" - }, - "test.short.ids.fasta:md5,14d6f587b6d28889c5c0f985e78d602f" - ] - ], - "short_ids_tsv": [ - [ - { - "id": "test" - }, - "test.short.ids.tsv:md5,d7a2af88e8549586e5616bff6a88bd71" - ] - ], - "versions": [ - "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba" - ] - } - ], - "timestamp": "2023-12-07T13:32:58.12885" - } -} \ No newline at end of file diff --git a/modules/pfr/custom/shortenfastaids/tests/tags.yml b/modules/pfr/custom/shortenfastaids/tests/tags.yml deleted file mode 100644 index 4715b64c..00000000 --- a/modules/pfr/custom/shortenfastaids/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -custom/shortenfastaids: - - "modules/pfr/custom/shortenfastaids/**" diff --git a/modules/pfr/edta/ltrharvest/environment.yml b/modules/pfr/edta/ltrharvest/environment.yml deleted file mode 100644 index ac836d2a..00000000 --- a/modules/pfr/edta/ltrharvest/environment.yml +++ /dev/null @@ -1,9 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json -name: "edta_ltrharvest" -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - "bioconda::edta=2.1.0" diff --git a/modules/pfr/edta/ltrharvest/main.nf b/modules/pfr/edta/ltrharvest/main.nf deleted file mode 100644 index 06887f1c..00000000 --- a/modules/pfr/edta/ltrharvest/main.nf +++ /dev/null @@ -1,55 +0,0 @@ -process EDTA_LTRHARVEST { - tag "$meta.id" - label 'process_medium' - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/edta:2.1.0--hdfd78af_1': - 'quay.io/biocontainers/edta:2.1.0--hdfd78af_1' }" - - input: - tuple val(meta), path(fasta) - - output: - tuple val(meta), path("*.gff3") , emit: gff3 - tuple val(meta), path("*.scn") , emit: scn - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - """ - /usr/local/share/EDTA/bin/LTR_HARVEST_parallel/LTR_HARVEST_parallel \\ - -seq $fasta \\ - $args \\ - -threads $task.cpus - - mv "${fasta}.harvest.combine.gff3" \\ - "${prefix}.gff3" - - mv "${fasta}.harvest.combine.scn" \\ - "${prefix}.scn" - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - LTR_HARVEST_parallel: \$(/usr/local/share/EDTA/bin/LTR_HARVEST_parallel/LTR_HARVEST_parallel -h | sed -n '/Version/s/Version: //p') - genometools: \$(gt --version | sed '1!d ; s/gt (GenomeTools) //') - END_VERSIONS - """ - - stub: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch "${prefix}.gff3" - touch "${prefix}.scn" - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - LTR_HARVEST_parallel: \$(/usr/local/share/EDTA/bin/LTR_HARVEST_parallel/LTR_HARVEST_parallel -h | sed -n '/Version/s/Version: //p') - genometools: \$(gt --version | sed '1!d ; s/gt (GenomeTools) //') - END_VERSIONS - """ -} diff --git a/modules/pfr/edta/ltrharvest/meta.yml b/modules/pfr/edta/ltrharvest/meta.yml deleted file mode 100644 index 1b6a969c..00000000 --- a/modules/pfr/edta/ltrharvest/meta.yml +++ /dev/null @@ -1,60 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: "edta_ltrharvest" -description: | - Predicts LTR retrotransposons using the parallel version of GenomeTools gt-ltrharvest - utility included in the EDTA toolchain -keywords: - - genomics - - genome - - annotation - - repeat - - transposons - - retrotransposons -tools: - - "edta": - description: Extensive de-novo TE Annotator (EDTA) - homepage: "https://github.com/oushujun/EDTA" - documentation: "https://github.com/oushujun/EDTA" - tool_dev_url: "https://github.com/oushujun/EDTA" - doi: "10.1186/s13059-019-1905-y" - licence: ["GPL v3"] - - "gt": - description: "The GenomeTools genome analysis system" - homepage: "https://genometools.org/index.html" - documentation: "https://genometools.org/documentation.html" - tool_dev_url: "https://github.com/genometools/genometools" - doi: "10.1109/TCBB.2013.68" - licence: ["ISC"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - fasta: - type: file - description: Input genome fasta - pattern: "*.{fsa,fa,fasta}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - - gff3: - type: file - description: Predicted LTR candidates in gff3 format - pattern: "*.gff3" - - scn: - type: file - description: Predicted LTR candidates in scn format - pattern: "*.scn" -authors: - - "@GallVp" -maintainers: - - "@GallVp" diff --git a/modules/pfr/edta/ltrharvest/tests/main.nf.test b/modules/pfr/edta/ltrharvest/tests/main.nf.test deleted file mode 100644 index 5f181873..00000000 --- a/modules/pfr/edta/ltrharvest/tests/main.nf.test +++ /dev/null @@ -1,60 +0,0 @@ -nextflow_process { - - name "Test Process EDTA_LTRHARVEST" - script "../main.nf" - process "EDTA_LTRHARVEST" - - tag "modules" - tag "modules_nfcore" - tag "edta" - tag "edta/ltrharvest" - - test("homo_sapiens-genome_21_fasta") { - - when { - process { - """ - input[0] = [ - [ id:'test' ], - file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert snapshot(path(process.out.versions[0]).text).match("script_versions") } - ) - } - - } - - test("homo_sapiens-genome_fasta-stub") { - - options '-stub' - - when { - process { - """ - input[0] = [ - [ id:'test' ], - file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert snapshot(path(process.out.versions[0]).text).match("stub_versions") } - ) - } - - } - -} \ No newline at end of file diff --git a/modules/pfr/edta/ltrharvest/tests/main.nf.test.snap b/modules/pfr/edta/ltrharvest/tests/main.nf.test.snap deleted file mode 100644 index 273db9a6..00000000 --- a/modules/pfr/edta/ltrharvest/tests/main.nf.test.snap +++ /dev/null @@ -1,120 +0,0 @@ -{ - "homo_sapiens-genome_21_fasta": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.gff3:md5,da13c4ba22e44ef944ddec38aa72c468" - ] - ], - "1": [ - [ - { - "id": "test" - }, - "test.scn:md5,65a6b80823a3f058142aed623a028a22" - ] - ], - "2": [ - "versions.yml:md5,da7912721842adc402c15740be57751f" - ], - "gff3": [ - [ - { - "id": "test" - }, - "test.gff3:md5,da13c4ba22e44ef944ddec38aa72c468" - ] - ], - "scn": [ - [ - { - "id": "test" - }, - "test.scn:md5,65a6b80823a3f058142aed623a028a22" - ] - ], - "versions": [ - "versions.yml:md5,da7912721842adc402c15740be57751f" - ] - } - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-02T21:08:07.746204" - }, - "homo_sapiens-genome_fasta-stub": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.gff3:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ], - "1": [ - [ - { - "id": "test" - }, - "test.scn:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ], - "2": [ - "versions.yml:md5,da7912721842adc402c15740be57751f" - ], - "gff3": [ - [ - { - "id": "test" - }, - "test.gff3:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ], - "scn": [ - [ - { - "id": "test" - }, - "test.scn:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ], - "versions": [ - "versions.yml:md5,da7912721842adc402c15740be57751f" - ] - } - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-02T21:08:13.587479" - }, - "script_versions": { - "content": [ - "\"EDTA_LTRHARVEST\":\n LTR_HARVEST_parallel: v1.1\n genometools: 1.6.2\n" - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-02T21:08:07.763375" - }, - "stub_versions": { - "content": [ - "\"EDTA_LTRHARVEST\":\n LTR_HARVEST_parallel: v1.1\n genometools: 1.6.2\n" - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-02T21:08:13.601529" - } -} \ No newline at end of file diff --git a/modules/pfr/edta/ltrharvest/tests/tags.yml b/modules/pfr/edta/ltrharvest/tests/tags.yml deleted file mode 100644 index a625c6e8..00000000 --- a/modules/pfr/edta/ltrharvest/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -edta/ltrharvest: - - "modules/pfr/edta/ltrharvest/**" diff --git a/modules/pfr/gt/gff3/environment.yml b/modules/pfr/gt/gff3/environment.yml deleted file mode 100644 index 8289fb31..00000000 --- a/modules/pfr/gt/gff3/environment.yml +++ /dev/null @@ -1,9 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json -name: "gt_gff3" -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - "bioconda::genometools-genometools=1.6.5" diff --git a/modules/pfr/gt/gff3/main.nf b/modules/pfr/gt/gff3/main.nf deleted file mode 100644 index f1774d28..00000000 --- a/modules/pfr/gt/gff3/main.nf +++ /dev/null @@ -1,51 +0,0 @@ -process GT_GFF3 { - tag "$meta.id" - label 'process_single' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/genometools-genometools:1.6.5--py310h3db02ab_0': - 'quay.io/biocontainers/genometools-genometools:1.6.5--py310h3db02ab_0' }" - - input: - tuple val(meta), path(gff3) - - output: - tuple val(meta), path("*.gt.gff3") , emit: gt_gff3 , optional: true - tuple val(meta), path("*.error.log"), emit: error_log , optional: true - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - """ - gt \\ - gff3 \\ - $args \\ - "$gff3" \\ - > "${prefix}.gt.gff3" \\ - 2> "${prefix}.error.log" \\ - || echo "Errors from gt-gff3 printed to ${prefix}.error.log" - - if grep -q "gt gff3: error:" "${prefix}.error.log"; then - echo "gt-gff3 failed to parse $gff3" - - rm \\ - "${prefix}.gt.gff3" - else - echo "gt-gff3 successfully parsed $gff3" - - mv \\ - "${prefix}.error.log" \\ - gt_gff3.stderr - fi - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - genometools: \$(gt --version | head -1 | sed 's/gt (GenomeTools) //') - END_VERSIONS - """ -} diff --git a/modules/pfr/gt/gff3/meta.yml b/modules/pfr/gt/gff3/meta.yml deleted file mode 100644 index 5cecd8d0..00000000 --- a/modules/pfr/gt/gff3/meta.yml +++ /dev/null @@ -1,48 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: "gt_gff3" -description: "GenomeTools gt-gff3 utility to parse, possibly transform, and output GFF3 files" -keywords: - - genome - - gff3 - - annotation -tools: - - "gt": - description: "The GenomeTools genome analysis system" - homepage: "https://genometools.org/index.html" - documentation: "https://genometools.org/documentation.html" - tool_dev_url: "https://github.com/genometools/genometools" - doi: "10.1109/TCBB.2013.68" - licence: ["ISC"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test' ]` - - gff3: - type: file - description: Input gff3 file - pattern: "*.{gff,gff3}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test' ]` - - gt_gff3: - type: file - description: Parsed gff3 file produced only if there is no parsing error - pattern: "*.gt.gff3" - - error_log: - type: file - description: Error log if gt-gff3 failed to parse the input gff3 file - pattern: "*.error.log" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@gallvp" -maintainers: - - "@gallvp" diff --git a/modules/pfr/gt/gff3/tests/main.nf.test b/modules/pfr/gt/gff3/tests/main.nf.test deleted file mode 100644 index b57439ef..00000000 --- a/modules/pfr/gt/gff3/tests/main.nf.test +++ /dev/null @@ -1,61 +0,0 @@ -nextflow_process { - - name "Test Process GT_GFF3" - script "../main.nf" - process "GT_GFF3" - config "./nextflow.config" - - tag "modules" - tag "modules_nfcore" - tag "gt" - tag "gt/gff3" - - test("sarscov2-gff3-valid") { - when { - process { - """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert process.out.gt_gff3 != null }, - { assert process.out.error_log == [] } - ) - } - - } - - test("sarscov2-gff3-invalid") { - when { - process { - """ - input[0] = Channel.of( - '##gff-version 3', - 'chr22\tID=gene:ENSG00000233995;Name=AP000547.1' - ) - .collectFile(name: 'sample.gff3', newLine: true) - .map { file -> [ [ id:'test' ], file ] } - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert process.out.gt_gff3 == [] }, - { assert process.out.error_log != null }, - { assert path(process.out.error_log.get(0).get(1)).getText().contains("gt gff3: error:") } - ) - } - } - -} \ No newline at end of file diff --git a/modules/pfr/gt/gff3/tests/main.nf.test.snap b/modules/pfr/gt/gff3/tests/main.nf.test.snap deleted file mode 100644 index f31e8d1c..00000000 --- a/modules/pfr/gt/gff3/tests/main.nf.test.snap +++ /dev/null @@ -1,72 +0,0 @@ -{ - "sarscov2-gff3-invalid": { - "content": [ - { - "0": [ - - ], - "1": [ - [ - { - "id": "test" - }, - "test.error.log:md5,31e6117c516f936ec403f792c732bc76" - ] - ], - "2": [ - "versions.yml:md5,9753770dd19a2a306dcf16d4aaf049eb" - ], - "error_log": [ - [ - { - "id": "test" - }, - "test.error.log:md5,31e6117c516f936ec403f792c732bc76" - ] - ], - "gt_gff3": [ - - ], - "versions": [ - "versions.yml:md5,9753770dd19a2a306dcf16d4aaf049eb" - ] - } - ], - "timestamp": "2023-11-28T13:43:34.620429" - }, - "sarscov2-gff3-valid": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.gt.gff3:md5,2ae900237ace415557b8735fac088b85" - ] - ], - "1": [ - - ], - "2": [ - "versions.yml:md5,9753770dd19a2a306dcf16d4aaf049eb" - ], - "error_log": [ - - ], - "gt_gff3": [ - [ - { - "id": "test" - }, - "test.gt.gff3:md5,2ae900237ace415557b8735fac088b85" - ] - ], - "versions": [ - "versions.yml:md5,9753770dd19a2a306dcf16d4aaf049eb" - ] - } - ], - "timestamp": "2023-11-28T13:43:31.065832" - } -} \ No newline at end of file diff --git a/modules/pfr/gt/gff3/tests/nextflow.config b/modules/pfr/gt/gff3/tests/nextflow.config deleted file mode 100644 index af562267..00000000 --- a/modules/pfr/gt/gff3/tests/nextflow.config +++ /dev/null @@ -1,3 +0,0 @@ -process { - ext.args = '-tidy -retainids' -} diff --git a/modules/pfr/gt/gff3/tests/tags.yml b/modules/pfr/gt/gff3/tests/tags.yml deleted file mode 100644 index ae040309..00000000 --- a/modules/pfr/gt/gff3/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -gt/gff3: - - "modules/pfr/gt/gff3/**" diff --git a/modules/pfr/gt/gff3validator/environment.yml b/modules/pfr/gt/gff3validator/environment.yml deleted file mode 100644 index ea57ebe0..00000000 --- a/modules/pfr/gt/gff3validator/environment.yml +++ /dev/null @@ -1,9 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json -name: "gt_gff3validator" -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - "bioconda::genometools-genometools=1.6.5" diff --git a/modules/pfr/gt/gff3validator/main.nf b/modules/pfr/gt/gff3validator/main.nf deleted file mode 100644 index 92e5dc2a..00000000 --- a/modules/pfr/gt/gff3validator/main.nf +++ /dev/null @@ -1,50 +0,0 @@ -process GT_GFF3VALIDATOR { - tag "$meta.id" - label 'process_single' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/genometools-genometools:1.6.5--py310h3db02ab_0': - 'quay.io/biocontainers/genometools-genometools:1.6.5--py310h3db02ab_0' }" - - input: - tuple val(meta), path(gff3) - - output: - tuple val(meta), path('*.success.log') , emit: success_log , optional: true - tuple val(meta), path('*.error.log') , emit: error_log , optional: true - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - gt \\ - gff3validator \\ - "$gff3" \\ - > "${prefix}.success.log" \\ - 2> "${prefix}.error.log" \\ - || echo "Errors from gt-gff3validator printed to ${prefix}.error.log" - - if grep -q "input is valid GFF3" "${prefix}.success.log"; then - echo "Validation successful..." - - mv \\ - "${prefix}.error.log" \\ - gt_gff3validator.stderr - else - echo "Validation failed..." - - mv \\ - "${prefix}.success.log" \\ - gt_gff3validator.stdout - fi - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - genometools: \$(gt --version | head -1 | sed 's/gt (GenomeTools) //') - END_VERSIONS - """ -} diff --git a/modules/pfr/gt/gff3validator/meta.yml b/modules/pfr/gt/gff3validator/meta.yml deleted file mode 100644 index 3322faf9..00000000 --- a/modules/pfr/gt/gff3validator/meta.yml +++ /dev/null @@ -1,49 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: "gt_gff3validator" -description: "GenomeTools gt-gff3validator utility to strictly validate a GFF3 file" -keywords: - - genome - - gff3 - - annotation - - validation -tools: - - "gt": - description: "The GenomeTools genome analysis system" - homepage: "https://genometools.org/index.html" - documentation: "https://genometools.org/documentation.html" - tool_dev_url: "https://github.com/genometools/genometools" - doi: "10.1109/TCBB.2013.68" - licence: ["ISC"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test' ]` - - gff3: - type: file - description: Input gff3 file - pattern: "*.{gff,gff3}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test' ]` - - success_log: - type: file - description: Log file for successful validation - pattern: "*.success.log" - - error_log: - type: file - description: Log file for failed validation - pattern: "*.error.log" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@GallVp" -maintainers: - - "@GallVp" diff --git a/modules/pfr/gt/gff3validator/tests/main.nf.test b/modules/pfr/gt/gff3validator/tests/main.nf.test deleted file mode 100644 index 9addd7d8..00000000 --- a/modules/pfr/gt/gff3validator/tests/main.nf.test +++ /dev/null @@ -1,63 +0,0 @@ -nextflow_process { - - name "Test Process GT_GFF3VALIDATOR" - script "../main.nf" - process "GT_GFF3VALIDATOR" - - tag "modules" - tag "modules_nfcore" - tag "gt" - tag "gt/gff3validator" - - test("custom-gff3-valid") { - - when { - process { - """ - input[0] = Channel.of( - '##gff-version 3', - 'chr22\thavana\tpseudogene\t16572027\t16574637\t.\t+\t.\tID=gene:ENSG00000233995;Name=AP000547.1' - ) - .collectFile(name: 'sample.gff3', newLine: true) - .map { file -> [ [ id:'test' ], file ] } - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert process.out.error_log == [] }, - { assert process.out.success_log != null }, - { assert path(process.out.success_log.get(0).get(1)).getText().contains("input is valid GFF3") } - ) - } - - } - - test("sarscov2-gff3-invalid") { - - when { - process { - """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['homo_sapiens']['genome']['genome_gff3'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert process.out.success_log == [] }, - { assert process.out.error_log != null }, - { assert path(process.out.error_log.get(0).get(1)).getText().contains("gt gff3validator: error:") } - ) - } - - } -} \ No newline at end of file diff --git a/modules/pfr/gt/gff3validator/tests/main.nf.test.snap b/modules/pfr/gt/gff3validator/tests/main.nf.test.snap deleted file mode 100644 index 0b6f065a..00000000 --- a/modules/pfr/gt/gff3validator/tests/main.nf.test.snap +++ /dev/null @@ -1,72 +0,0 @@ -{ - "sarscov2-gff3-invalid": { - "content": [ - { - "0": [ - - ], - "1": [ - [ - { - "id": "test" - }, - "test.error.log:md5,c5d16b263a87072a13cca44fd811b8e2" - ] - ], - "2": [ - "versions.yml:md5,5927673eb73a8c22408643d224414215" - ], - "error_log": [ - [ - { - "id": "test" - }, - "test.error.log:md5,c5d16b263a87072a13cca44fd811b8e2" - ] - ], - "success_log": [ - - ], - "versions": [ - "versions.yml:md5,5927673eb73a8c22408643d224414215" - ] - } - ], - "timestamp": "2023-11-29T11:09:23.708792" - }, - "custom-gff3-valid": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.success.log:md5,b11ca5c18c865fc808ea0fef0b07da30" - ] - ], - "1": [ - - ], - "2": [ - "versions.yml:md5,5927673eb73a8c22408643d224414215" - ], - "error_log": [ - - ], - "success_log": [ - [ - { - "id": "test" - }, - "test.success.log:md5,b11ca5c18c865fc808ea0fef0b07da30" - ] - ], - "versions": [ - "versions.yml:md5,5927673eb73a8c22408643d224414215" - ] - } - ], - "timestamp": "2023-11-29T11:09:19.530068" - } -} \ No newline at end of file diff --git a/modules/pfr/gt/gff3validator/tests/tags.yml b/modules/pfr/gt/gff3validator/tests/tags.yml deleted file mode 100644 index e247d55e..00000000 --- a/modules/pfr/gt/gff3validator/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -gt/gff3validator: - - "modules/pfr/gt/gff3validator/**" diff --git a/modules/pfr/gt/stat/environment.yml b/modules/pfr/gt/stat/environment.yml deleted file mode 100644 index dca959b3..00000000 --- a/modules/pfr/gt/stat/environment.yml +++ /dev/null @@ -1,9 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json -name: "gt_stat" -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - "bioconda::genometools-genometools=1.6.5" diff --git a/modules/pfr/gt/stat/main.nf b/modules/pfr/gt/stat/main.nf deleted file mode 100644 index ad8da8a9..00000000 --- a/modules/pfr/gt/stat/main.nf +++ /dev/null @@ -1,35 +0,0 @@ -process GT_STAT { - tag "$meta.id" - label 'process_single' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/genometools-genometools:1.6.5--py310h3db02ab_0': - 'quay.io/biocontainers/genometools-genometools:1.6.5--py310h3db02ab_0' }" - - input: - tuple val(meta), path(gff3) - - output: - tuple val(meta), path("*.gt.stat.yml") , emit: stats - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - """ - gt \\ - stat \\ - $args \\ - "$gff3" \\ - > "${prefix}.gt.stat.yml" - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - genometools: \$(gt --version | head -1 | sed 's/gt (GenomeTools) //') - END_VERSIONS - """ -} diff --git a/modules/pfr/gt/stat/meta.yml b/modules/pfr/gt/stat/meta.yml deleted file mode 100644 index 203059a6..00000000 --- a/modules/pfr/gt/stat/meta.yml +++ /dev/null @@ -1,46 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: "gt_stat" -description: "GenomeTools gt-stat utility to show statistics about features contained in GFF3 files" -keywords: - - genome - - gff3 - - annotation - - statistics - - stats -tools: - - "gt": - description: "The GenomeTools genome analysis system" - homepage: "https://genometools.org/index.html" - documentation: "https://genometools.org/documentation.html" - tool_dev_url: "https://github.com/genometools/genometools" - doi: "10.1109/TCBB.2013.68" - licence: ["ISC"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test' ]` - - gff3: - type: file - description: Input gff3 file - pattern: "*.{gff,gff3}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test' ]` - - stats: - type: file - description: Stats file in yaml format - pattern: "*.gt.stat.yml" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@GallVp" -maintainers: - - "@GallVp" diff --git a/modules/pfr/gt/stat/tests/main.nf.test b/modules/pfr/gt/stat/tests/main.nf.test deleted file mode 100644 index 8736d013..00000000 --- a/modules/pfr/gt/stat/tests/main.nf.test +++ /dev/null @@ -1,37 +0,0 @@ -nextflow_process { - - name "Test Process GT_STAT" - script "../main.nf" - process "GT_STAT" - - tag "modules" - tag "modules_nfcore" - tag "gt" - tag "gt/stat" - - test("sarscov2-gff3") { - - when { - process { - """ - input[0] = Channel.of( - "##gff-version 3" + - file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true).getText().toLowerCase() - ) - .collectFile(name: 'sample.gff3', newLine: true) - .map { file -> [ [ id:'test' ], file ] } - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert path(process.out.stats.get(0).get(1)).getText().contains("cdss: 12") } - ) - } - - } - -} \ No newline at end of file diff --git a/modules/pfr/gt/stat/tests/main.nf.test.snap b/modules/pfr/gt/stat/tests/main.nf.test.snap deleted file mode 100644 index 2fcfb8a8..00000000 --- a/modules/pfr/gt/stat/tests/main.nf.test.snap +++ /dev/null @@ -1,31 +0,0 @@ -{ - "sarscov2-gff3": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.gt.stat.yml:md5,ebba7831ddbf916b8bbea675ba8693b5" - ] - ], - "1": [ - "versions.yml:md5,a184b50afb2ad6dd2d3d37b0a211dd71" - ], - "stats": [ - [ - { - "id": "test" - }, - "test.gt.stat.yml:md5,ebba7831ddbf916b8bbea675ba8693b5" - ] - ], - "versions": [ - "versions.yml:md5,a184b50afb2ad6dd2d3d37b0a211dd71" - ] - } - ], - "timestamp": "2023-11-29T11:34:48.057277" - } -} \ No newline at end of file diff --git a/modules/pfr/gt/stat/tests/tags.yml b/modules/pfr/gt/stat/tests/tags.yml deleted file mode 100644 index 46be6341..00000000 --- a/modules/pfr/gt/stat/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -gt/stat: - - "modules/pfr/gt/stat/**" diff --git a/modules/pfr/lai/environment.yml b/modules/pfr/lai/environment.yml deleted file mode 100644 index 94fadbd2..00000000 --- a/modules/pfr/lai/environment.yml +++ /dev/null @@ -1,9 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json -name: "lai" -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - "bioconda::LTR_retriever=2.9.0" diff --git a/modules/pfr/lai/main.nf b/modules/pfr/lai/main.nf deleted file mode 100644 index 7d2cf3be..00000000 --- a/modules/pfr/lai/main.nf +++ /dev/null @@ -1,68 +0,0 @@ -process LAI { - tag "$meta.id" - label 'process_high' - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/ltr_retriever:2.9.0--hdfd78af_2': - 'quay.io/biocontainers/ltr_retriever:2.9.0--hdfd78af_2' }" - - input: - tuple val(meta), path(fasta) - path pass_list - path annotation_out - path monoploid_seqs - - output: - tuple val(meta), path("*.LAI.log") , emit: log - tuple val(meta), path("*.LAI.out") , emit: lai_out , optional: true - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def monoploid_param = monoploid_seqs ? "-mono $monoploid_seqs" : '' - def lai_output_name = monoploid_seqs ? "${annotation_out}.${monoploid_seqs}.out.LAI" : "${annotation_out}.LAI" - """ - # Remove comments from genome fasta, - # otherwise LAI triggers its sequence name change logic - - sed \\ - '/^>/ s/\\s.*\$//' \\ - $fasta \\ - > for_lai_no_comments.fsa - - LAI \\ - -genome for_lai_no_comments.fsa \\ - -intact $pass_list \\ - -all $annotation_out \\ - -t $task.cpus \\ - $monoploid_param \\ - $args \\ - > "${prefix}.LAI.log" - - mv \\ - $lai_output_name \\ - "${prefix}.LAI.out" \\ - || echo "LAI did not produce the output file" - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - lai: \$(cat /usr/local/share/LTR_retriever/LAI | grep "my \\\$version" | sed 's/my \$version="//; s/";//') - END_VERSIONS - """ - - stub: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.LAI.log - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - lai: \$(cat /usr/local/share/LTR_retriever/LAI | grep "my \\\$version" | sed 's/my \$version="//; s/";//') - END_VERSIONS - """ -} diff --git a/modules/pfr/lai/meta.yml b/modules/pfr/lai/meta.yml deleted file mode 100644 index 6fd7aef6..00000000 --- a/modules/pfr/lai/meta.yml +++ /dev/null @@ -1,68 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: "lai" -description: Estimates the mean LTR sequence identity in the genome -keywords: - - genomics - - annotation - - repeat - - long terminal retrotransposon - - retrotransposon - - stats - - qc -tools: - - "lai": - description: Assessing genome assembly quality using the LTR Assembly Index (LAI) - homepage: "https://github.com/oushujun/LTR_retriever" - documentation: "https://github.com/oushujun/LTR_retriever" - tool_dev_url: "https://github.com/oushujun/LTR_retriever" - doi: "10.1093/nar/gky730" - licence: ["GPL v3"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - fasta: - type: file - description: The genome file that is used to generate everything - pattern: "*.{fsa,fa,fasta}" - - pass_list: - type: file - description: A list of intact LTR-RTs generated by LTR_retriever - pattern: "*.pass.list" - - annotation_out: - type: file - description: RepeatMasker annotation of all LTR sequences in the genome - pattern: "*.out" - - monoploid_seqs: - type: file - description: | - This parameter is mainly for ployploid genomes. User provides a list of - sequence names that represent a monoploid (1x). LAI will be calculated only - on these sequences if provided. - pattern: "*.txt" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1', single_end:false ]` - - log: - type: file - description: Log from LAI - pattern: "*.LAI.log" - - lai_out: - type: file - description: | - Output file from LAI if LAI is able to estimate the index from the inputs - pattern: "*.LAI.out" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@GallVp" -maintainers: - - "@GallVp" diff --git a/modules/pfr/lai/tests/main.nf.test b/modules/pfr/lai/tests/main.nf.test deleted file mode 100644 index 0787d6b6..00000000 --- a/modules/pfr/lai/tests/main.nf.test +++ /dev/null @@ -1,120 +0,0 @@ -nextflow_process { - - name "Test Process LAI" - script "../main.nf" - process "LAI" - config "./nextflow.config" - - tag "modules" - tag "modules_nfcore" - tag "lai" - tag "gt/suffixerator" - tag "nf-core/gunzip" - tag "gt/ltrharvest" - tag "ltrretriever" - - test("homo_sapiens-genome_21_fasta-success") { - - setup { - run("GUNZIP") { - script "../../../nf-core/gunzip" - - process { - """ - input[0] = [ - [ id:'test' ], - file('/Users/hrauxr/Projects/nxf-modules/tests/data/chr1.fa.gz', checkIfExists: true) - ] - """ - } - } - - run("GT_SUFFIXERATOR") { - script "../../../pfr/gt/suffixerator" - - process { - """ - input[0] = GUNZIP.out.gunzip - """ - } - } - - run("GT_LTRHARVEST") { - script "../../../pfr/gt/ltrharvest" - - process { - """ - input[0] = GT_SUFFIXERATOR.out.index - """ - } - } - - run("LTRRETRIEVER") { - script "../../../pfr/ltrretriever" - - process { - """ - input[0] = GUNZIP.out.gunzip - input[1] = GT_LTRHARVEST.out.tabout.map { meta, tabout -> tabout } - input[2] = [] - input[3] = [] - input[4] = [] - """ - } - } - } - - when { - process { - """ - input[0] = GUNZIP.out.gunzip - input[1] = LTRRETRIEVER.out.pass_list.map { meta, pass_list -> pass_list } - input[2] = LTRRETRIEVER.out.annotation_out.map { meta, annotation_out -> annotation_out } - input[3] = [] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert path(process.out.log.get(0).get(1)).getText().contains("Dependency checking: Passed!") }, - { assert path(process.out.log.get(0).get(1)).getText().contains("Calculate LAI:") }, - { assert path(process.out.log.get(0).get(1)).getText().contains("Total LTR sequence content (0%) is too low for accurate LAI calculation") }, - { assert path(process.out.log.get(0).get(1)).getText().contains("Sorry, LAI is not applicable on the current genome assembly.") }, - { assert process.out.lai_out == [] }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - - test("stub") { - - options '-stub' - - when { - process { - """ - input[0] = [ - [ id:'test' ], - file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) - ] - input[1] = [] - input[2] = [] - input[3] = [] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - -} \ No newline at end of file diff --git a/modules/pfr/lai/tests/main.nf.test.snap b/modules/pfr/lai/tests/main.nf.test.snap deleted file mode 100644 index 751ddb60..00000000 --- a/modules/pfr/lai/tests/main.nf.test.snap +++ /dev/null @@ -1,10 +0,0 @@ -{ - "versions": { - "content": [ - [ - "versions.yml:md5,2ac93e1e6324236af6f9a794bbac2099" - ] - ], - "timestamp": "2023-12-05T12:15:32.969684" - } -} \ No newline at end of file diff --git a/modules/pfr/lai/tests/nextflow.config b/modules/pfr/lai/tests/nextflow.config deleted file mode 100644 index 516a3e27..00000000 --- a/modules/pfr/lai/tests/nextflow.config +++ /dev/null @@ -1,10 +0,0 @@ -process { - - withName: GT_SUFFIXERATOR { - ext.args = '-tis -suf -lcp -des -ssp -sds -dna' - } - - withName: GT_LTRHARVEST { - ext.args = '-minlenltr 100 -maxlenltr 7000 -mintsd 4 -maxtsd 6 -motif TGCA -motifmis 1 -similar 85 -vic 10 -seed 20 -seqids yes' - } -} diff --git a/modules/pfr/lai/tests/tags.yml b/modules/pfr/lai/tests/tags.yml deleted file mode 100644 index 252295d7..00000000 --- a/modules/pfr/lai/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -lai: - - "modules/pfr/lai/**" diff --git a/modules/pfr/ltrfinder/environment.yml b/modules/pfr/ltrfinder/environment.yml deleted file mode 100644 index 3ffa7c33..00000000 --- a/modules/pfr/ltrfinder/environment.yml +++ /dev/null @@ -1,9 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json -name: "ltrfinder" -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - "bioconda::edta=2.1.0" diff --git a/modules/pfr/ltrfinder/main.nf b/modules/pfr/ltrfinder/main.nf deleted file mode 100644 index 6555c235..00000000 --- a/modules/pfr/ltrfinder/main.nf +++ /dev/null @@ -1,52 +0,0 @@ -process LTRFINDER { - tag "$meta.id" - label 'process_high' - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/edta:2.1.0--hdfd78af_1': - 'quay.io/biocontainers/edta:2.1.0--hdfd78af_1' }" - - input: - tuple val(meta), path(fasta) - - output: - tuple val(meta), path("*.scn") , emit: scn - tuple val(meta), path("*.gff3") , emit: gff - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - """ - /usr/local/share/EDTA/bin/LTR_FINDER_parallel/LTR_FINDER_parallel \\ - -seq $fasta \\ - -threads $task.cpus \\ - $args - - mv "${fasta}.finder.combine.scn" "${prefix}.scn" - mv "${fasta}.finder.combine.gff3" "${prefix}.gff3" - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - LTR_FINDER_parallel: \$(/usr/local/share/EDTA/bin/LTR_FINDER_parallel/LTR_FINDER_parallel -h | grep 'Version:' | sed 's/Version: //') - ltr_finder: \$(ltr_finder -h 2>&1 | grep 'ltr_finder' | sed 's/ltr_finder //') - END_VERSIONS - """ - - stub: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch "${prefix}.scn" - touch "${prefix}.gff3" - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - LTR_FINDER_parallel: \$(/usr/local/share/EDTA/bin/LTR_FINDER_parallel/LTR_FINDER_parallel -h | grep 'Version:' | sed 's/Version: //') - ltr_finder: \$(ltr_finder -h 2>&1 | grep 'ltr_finder' | sed 's/ltr_finder //') - END_VERSIONS - """ -} diff --git a/modules/pfr/ltrfinder/meta.yml b/modules/pfr/ltrfinder/meta.yml deleted file mode 100644 index 7fdfa5f9..00000000 --- a/modules/pfr/ltrfinder/meta.yml +++ /dev/null @@ -1,60 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: "ltrfinder" -description: | - Finds full-length LTR retrotranspsons in genome sequences using the - parallel version of LTR_Finder -keywords: - - genomics - - annotation - - parallel - - repeat - - long terminal retrotransposon - - retrotransposon -tools: - - "LTR_FINDER_parallel": - description: A Perl wrapper for LTR_FINEDR - homepage: "https://github.com/oushujun/LTR_FINDER_parallel" - documentation: "https://github.com/oushujun/LTR_FINDER_parallel" - tool_dev_url: "https://github.com/oushujun/LTR_FINDER_parallel" - doi: "10.1186/s13100-019-0193-0" - licence: ["MIT"] - - "LTR_Finder": - description: An efficient program for finding full-length LTR retrotranspsons in genome sequences - homepage: "https://github.com/xzhub/LTR_Finder" - documentation: "https://github.com/xzhub/LTR_Finder" - tool_dev_url: "https://github.com/xzhub/LTR_Finder" - doi: "10.1093/nar/gkm286" - licence: ["MIT"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - fasta: - type: file - description: Genome sequences in fasta format - pattern: "*.{fsa,fa,fasta}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - scn: - type: file - description: Annotation in LTRharvest or LTR_FINDER format - pattern: "*.scn" - - gff: - type: file - description: Annotation in gff3 format - pattern: "*.gff3" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@GallVp" -maintainers: - - "@GallVp" diff --git a/modules/pfr/ltrfinder/tests/main.nf.test b/modules/pfr/ltrfinder/tests/main.nf.test deleted file mode 100644 index 9b2fbb0b..00000000 --- a/modules/pfr/ltrfinder/tests/main.nf.test +++ /dev/null @@ -1,60 +0,0 @@ -nextflow_process { - - name "Test Process LTRFINDER" - script "../main.nf" - process "LTRFINDER" - - tag "modules" - tag "modules_nfcore" - tag "ltrfinder" - - test("homo_sapiens-genome_fasta-success") { - - when { - process { - """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - - test("stub") { - - options "-stub" - - when { - process { - """ - input[0] = [ - [ id:'test' ], // meta map - file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert process.out.scn != null }, - { assert process.out.gff != null }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - -} \ No newline at end of file diff --git a/modules/pfr/ltrfinder/tests/main.nf.test.snap b/modules/pfr/ltrfinder/tests/main.nf.test.snap deleted file mode 100644 index c0b7a205..00000000 --- a/modules/pfr/ltrfinder/tests/main.nf.test.snap +++ /dev/null @@ -1,55 +0,0 @@ -{ - "versions": { - "content": [ - [ - "versions.yml:md5,2cff73621bfc5c4abc40613c33bd92b8" - ] - ], - "timestamp": "2023-12-05T09:09:31.335554" - }, - "homo_sapiens-genome_fasta-success": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.scn:md5,2ce449dff751e59dbc292b6888491954" - ] - ], - "1": [ - [ - { - "id": "test" - }, - "test.gff3:md5,a91c388a54d7694bd14a4b085935759c" - ] - ], - "2": [ - "versions.yml:md5,2cff73621bfc5c4abc40613c33bd92b8" - ], - "gff": [ - [ - { - "id": "test" - }, - "test.gff3:md5,a91c388a54d7694bd14a4b085935759c" - ] - ], - "scn": [ - [ - { - "id": "test" - }, - "test.scn:md5,2ce449dff751e59dbc292b6888491954" - ] - ], - "versions": [ - "versions.yml:md5,2cff73621bfc5c4abc40613c33bd92b8" - ] - } - ], - "timestamp": "2023-12-05T09:09:31.32397" - } -} \ No newline at end of file diff --git a/modules/pfr/ltrfinder/tests/tags.yml b/modules/pfr/ltrfinder/tests/tags.yml deleted file mode 100644 index 4f613338..00000000 --- a/modules/pfr/ltrfinder/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -ltrfinder: - - "modules/pfr/ltrfinder/**" diff --git a/modules/pfr/ltrretriever/environment.yml b/modules/pfr/ltrretriever/environment.yml deleted file mode 100644 index 8e870fdc..00000000 --- a/modules/pfr/ltrretriever/environment.yml +++ /dev/null @@ -1,9 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json -name: "ltrretriever" -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - "bioconda::LTR_retriever=2.9.0" diff --git a/modules/pfr/ltrretriever/main.nf b/modules/pfr/ltrretriever/main.nf deleted file mode 100644 index 1e673dd5..00000000 --- a/modules/pfr/ltrretriever/main.nf +++ /dev/null @@ -1,76 +0,0 @@ -process LTRRETRIEVER { - tag "$meta.id" - label 'process_high' - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/ltr_retriever:2.9.0--hdfd78af_2': - 'quay.io/biocontainers/ltr_retriever:2.9.0--hdfd78af_2' }" - - input: - tuple val(meta), path(genome) - path(harvest) - path(finder) - path(mgescan) - path(non_tgca) - - output: - tuple val(meta), path("*.log") , emit: log - tuple val(meta), path("${prefix}.pass.list"), emit: pass_list - tuple val(meta), path("*.pass.list.gff3") , emit: pass_list_gff - tuple val(meta), path("*.LTRlib.fa") , emit: ltrlib - tuple val(meta), path("${prefix}.out") , emit: annotation_out , optional: true - tuple val(meta), path("*.out.gff3") , emit: annotation_gff , optional: true - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - prefix = task.ext.prefix ?: "${meta.id}" - def inharvest = harvest ? "-inharvest $harvest" : '' - def infinder = finder ? "-infinder $finder" : '' - def inmgescan = mgescan ? "-inmgescan $mgescan" : '' - def non_tgca_file = non_tgca ? "-nonTGCA $non_tgca" : '' - """ - LTR_retriever \\ - -genome $genome \\ - $inharvest \\ - $infinder \\ - $inmgescan \\ - $non_tgca_file \\ - -threads $task.cpus \\ - $args \\ - &> >(tee "${prefix}.log" 2>&1) - - mv "${genome}.pass.list" "${prefix}.pass.list" - mv "${genome}.pass.list.gff3" "${prefix}.pass.list.gff3" - mv "${genome}.LTRlib.fa" "${prefix}.LTRlib.fa" - mv "${genome}.out" "${prefix}.out" || echo ".out was not produced" - mv "${genome}.out.gff3" "${prefix}.out.gff3" || echo ".out.gff3 was not produced" - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - LTR_retriever: \$(LTR_retriever -h 2>&1 | grep '### LTR_retriever' | sed 's/### LTR_retriever //; s/ ###//') - END_VERSIONS - """ - - stub: - def args = task.ext.args ?: '' - prefix = task.ext.prefix ?: "${meta.id}" - def touch_out = args.contains('-noanno') ? '' : "touch ${prefix}.out" - def touch_out_gff = args.contains('-noanno') ? '' : "touch ${prefix}.out.gff3" - """ - touch "${prefix}.log" - touch "${prefix}.pass.list" - touch "${prefix}.pass.list.gff3" - touch "${prefix}.LTRlib.fa" - $touch_out - $touch_out_gff - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - LTR_retriever: \$(LTR_retriever -h 2>&1 | grep '### LTR_retriever' | sed 's/### LTR_retriever //; s/ ###//') - END_VERSIONS - """ -} diff --git a/modules/pfr/ltrretriever/meta.yml b/modules/pfr/ltrretriever/meta.yml deleted file mode 100644 index c0bfc240..00000000 --- a/modules/pfr/ltrretriever/meta.yml +++ /dev/null @@ -1,83 +0,0 @@ ---- -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: "ltrretriever" -description: write your description here -keywords: - - genomics - - annotation - - repeat - - long terminal repeat - - retrotransposon -tools: - - "LTR_retriever": - description: Sensitive and accurate identification of LTR retrotransposons - homepage: "https://github.com/oushujun/LTR_retriever" - documentation: "https://github.com/oushujun/LTR_retriever" - tool_dev_url: "https://github.com/oushujun/LTR_retriever" - doi: "10.1104/pp.17.01310" - licence: ["GPL v3"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - genome: - type: file - description: Genomic sequences in fasta format - pattern: "*.{fsa,fa,fasta}" - - harvest: - type: file - description: LTR-RT candidates from GenomeTools ltrharvest in the old tabular format - pattern: "*.tabout" - - finder: - type: file - description: LTR-RT candidates from LTR_FINDER - pattern: "*.scn" - - mgescan: - type: file - description: LTR-RT candidates from MGEScan_LTR - pattern: "*.out" - - non_tgca: - type: file - description: Non-canonical LTR-RT candidates from GenomeTools ltrharvest in the old tabular format - pattern: "*.tabout" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'sample1' ]` - - log: - type: file - description: Output log from LTR_retriever - pattern: "*.log" - - pass_list: - type: file - description: Intact LTR-RTs with coordinate and structural information in summary table format - pattern: "*.pass.list" - - pass_list_gff: - type: file - description: Intact LTR-RTs with coordinate and structural information in gff3 format - pattern: "*.pass.list.gff3" - - ltrlib: - type: file - description: All non-redundant LTR-RTs - pattern: "*.LTRlib.fa" - - annotation_out: - type: file - description: Whole-genome LTR-RT annotation by the non-redundant library - pattern: "*.out" - - annotation_gff: - type: file - description: Whole-genome LTR-RT annotation by the non-redundant library in gff3 format - pattern: "*.out.gff3" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - -authors: - - "@GallVp" -maintainers: - - "@GallVp" diff --git a/modules/pfr/ltrretriever/tests/main.nf.test b/modules/pfr/ltrretriever/tests/main.nf.test deleted file mode 100644 index 2121e7f1..00000000 --- a/modules/pfr/ltrretriever/tests/main.nf.test +++ /dev/null @@ -1,104 +0,0 @@ -nextflow_process { - - name "Test Process LTRRETRIEVER" - script "../main.nf" - process "LTRRETRIEVER" - config "./nextflow.config" - - tag "modules" - tag "modules_nfcore" - tag "ltrretriever" - tag "gt/ltrharvest" - tag "gt/suffixerator" - - test("homo_sapiens-genome_21_fasta-success") { - - setup { - run("GT_SUFFIXERATOR") { - script "../../../pfr/gt/suffixerator" - - process { - """ - input[0] = [ - [ id:'test' ], - file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) - ] - """ - } - } - - run("GT_LTRHARVEST") { - script "../../../pfr/gt/ltrharvest" - - process { - """ - input[0] = GT_SUFFIXERATOR.out.index - """ - } - } - } - - when { - process { - """ - input[0] = [ - [ id:'test' ], - file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) - ] - input[1] = GT_LTRHARVEST.out.tabout.map { meta, tabout -> tabout } - input[2] = [] - input[3] = [] - input[4] = [] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out.pass_list).match("pass_list") }, - { assert path(process.out.pass_list_gff.get(0).get(1)).getText().contains("chr21\tLTR_retriever\ttarget_site_duplication\t40960698\t40960702") }, - { assert process.out.ltrlib != null }, - { assert process.out.annotation_out == [] }, - { assert process.out.annotation_gff == [] }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - - test("stub") { - - options '-stub' - - when { - process { - """ - input[0] = [ - [ id:'test' ], - file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) - ] - input[1] = [] - input[2] = [] - input[3] = [] - input[4] = [] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out.versions).match("versions") }, - { assert process.out.log != null }, - { assert process.out.pass_list != null }, - { assert process.out.pass_list_gff != null }, - { assert process.out.ltrlib != null }, - { assert process.out.annotation_out == [] }, - { assert process.out.annotation_gff == [] } - ) - } - - } - -} \ No newline at end of file diff --git a/modules/pfr/ltrretriever/tests/main.nf.test.snap b/modules/pfr/ltrretriever/tests/main.nf.test.snap deleted file mode 100644 index a6e196bc..00000000 --- a/modules/pfr/ltrretriever/tests/main.nf.test.snap +++ /dev/null @@ -1,23 +0,0 @@ -{ - "pass_list": { - "content": [ - [ - [ - { - "id": "test" - }, - "test.pass.list:md5,4e99412b54fd99cac2ae533a51cbd4e1" - ] - ] - ], - "timestamp": "2023-12-04T15:45:22.007912" - }, - "versions": { - "content": [ - [ - "versions.yml:md5,bd6cf2de800197f0d726ba1dfa1d6be4" - ] - ], - "timestamp": "2023-12-04T15:45:22.026825" - } -} \ No newline at end of file diff --git a/modules/pfr/ltrretriever/tests/nextflow.config b/modules/pfr/ltrretriever/tests/nextflow.config deleted file mode 100644 index 22183f52..00000000 --- a/modules/pfr/ltrretriever/tests/nextflow.config +++ /dev/null @@ -1,14 +0,0 @@ -process { - - withName: GT_SUFFIXERATOR { - ext.args = '-tis -suf -lcp -des -ssp -sds -dna' - } - - withName: GT_LTRHARVEST { - ext.args = '-minlenltr 100 -maxlenltr 7000 -mintsd 4 -maxtsd 6 -motif TGCA -motifmis 1 -similar 85 -vic 10 -seed 20 -seqids yes' - } - - withName: LTRRETRIEVER { - ext.args = '-noanno' - } -} diff --git a/modules/pfr/ltrretriever/tests/tags.yml b/modules/pfr/ltrretriever/tests/tags.yml deleted file mode 100644 index 1837f0db..00000000 --- a/modules/pfr/ltrretriever/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -ltrretriever: - - "modules/pfr/ltrretriever/**" diff --git a/modules/pfr/samblaster/environment.yml b/modules/pfr/samblaster/environment.yml deleted file mode 100644 index f956283e..00000000 --- a/modules/pfr/samblaster/environment.yml +++ /dev/null @@ -1,8 +0,0 @@ -name: samblaster -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::samblaster=0.1.26 - - bioconda::samtools=1.16.1 diff --git a/modules/pfr/samblaster/main.nf b/modules/pfr/samblaster/main.nf deleted file mode 100644 index 160d1dd2..00000000 --- a/modules/pfr/samblaster/main.nf +++ /dev/null @@ -1,37 +0,0 @@ -process SAMBLASTER { - tag "$meta.id" - label 'process_low' - label "process_long" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/mulled-v2-19fa9f1a5c3966b63a24166365e81da35738c5ab:cee56b506ceb753d4bbef7e05b81e1bfc25d937f-0' : - 'quay.io/biocontainers/mulled-v2-19fa9f1a5c3966b63a24166365e81da35738c5ab:cee56b506ceb753d4bbef7e05b81e1bfc25d937f-0' }" - - input: - tuple val(meta), path(bam) - - output: - tuple val(meta), path("*.bam"), emit: bam - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def args2 = task.ext.args2 ?: '' - def args3 = task.ext.args3 ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - if( "$bam" == "${prefix}.bam" ) error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" - """ - samtools view -h $args2 $bam | \\ - samblaster $args | \\ - samtools view $args3 -Sb - >${prefix}.bam - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - samblaster: \$( samblaster -h 2>&1 | head -n 1 | sed 's/^samblaster: Version //' ) - samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') - END_VERSIONS - """ -} diff --git a/modules/pfr/samblaster/meta.yml b/modules/pfr/samblaster/meta.yml deleted file mode 100644 index ccb48320..00000000 --- a/modules/pfr/samblaster/meta.yml +++ /dev/null @@ -1,53 +0,0 @@ -name: samblaster -description: | - This module combines samtools and samblaster in order to use - samblaster capability to filter or tag SAM files, with the advantage - of maintaining both input and output in BAM format. - Samblaster input must contain a sequence header: for this reason it has been piped - with the "samtools view -h" command. - Additional desired arguments for samtools can be passed using: - options.args2 for the input bam file - options.args3 for the output bam file -keywords: - - sort - - duplicate marking - - bam -tools: - - samblaster: - description: | - samblaster is a fast and flexible program for marking duplicates in read-id grouped paired-end SAM files. - It can also optionally output discordant read pairs and/or split read mappings to separate SAM files, - and/or unmapped/clipped reads to a separate FASTQ file. - By default, samblaster reads SAM input from stdin and writes SAM to stdout. - documentation: https://github.com/GregoryFaust/samblaster - tool_dev_url: https://github.com/GregoryFaust/samblaster - doi: "10.1093/bioinformatics/btu314" - licence: ["MIT"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bam: - type: file - description: BAM file - pattern: "*.bam" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - - bam: - type: file - description: Tagged or filtered BAM file - pattern: "*.bam" -authors: - - "@lescai" -maintainers: - - "@lescai" diff --git a/nextflow.config b/nextflow.config index 8ade16d1..3db97ece 100644 --- a/nextflow.config +++ b/nextflow.config @@ -1,237 +1,275 @@ -includeConfig './conf/base.config' - +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + plant-food-research-open/assemblyqc Nextflow config file +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Default config options for all compute environments +---------------------------------------------------------------------------------------- +*/ + +// Global default params, used in configs params { - // FASTA files (fasta, fasta.gz) for the assemblies to QC - // - // Pattern: [["tag", "file path"]] - // Permissible tags: tag, tag_1, tag_tag2_3, tag_tag2_tag3; - // Any name with alphanumeric characters including "_". - // "." is not allowed in the tag name - // Unique, short tags are recommended. - // Otherwise, some of the plots in the report may not display correctly. - // Examples: - // target_assemblies = [["tag1", "./a/relative/path/to/the/fasta/file.fasta"], - // ["tag2", "./a/relative/path/to/the/fasta/file2.fasta"], - // ["tag3", "https://ftp.ncbi.nlm.nih.gov/genomes/test_genome.fna"], ...] - // target_assemblies = [["tair10", "/an/absolute/path/to/the/fasta/file.fasta"]] - target_assemblies = [ - ["hap1", "/workspace/assembly_qc/test_data/default/test_data1.fasta.gz"], - ["hap2", "/workspace/assembly_qc/test_data/default/test_data2.fasta"] - ] - - // GFF3 files (gff3, gff3.gz) for the assemblies to QC - // - // Optional Set to [] if not needed such as assembly_gff3 = [] - // - // Not all assembly gff3 files have to be provided such as: - // assembly_gff3 = [["hap1", "/workspace/assembly_qc/test_data/default/test_data1.gff3"]] - // - // Each gff3 file should have an associated (by tag) fasta file in target_assemblies. - // - // Multiple gff3 files can be associated (by tag) with a single fasta file in target_assemblies. - assembly_gff3 = [ - ["hap1", "/workspace/assembly_qc/test_data/default/test_data1.gff3.gz"], - ["hap2", "/workspace/assembly_qc/test_data/default/test_data2.gff3"] - ] - - assemblathon_stats { - // The number of 'N's for the unknown gap size. - // This number is used to split the scaffolds into contigs - // to compute contig-related stats such as the number of contigs, N50, etc. - // NCBI recommendation(https://www.ncbi.nlm.nih.gov/genbank/wgs_gapped/) is 100 - n_limit = 100 - } - - ncbi_fcs_adaptor { - skip = 0 // 1: Skip, 0: Don't - - empire = 'euk' // euk: Eukaryote, prok: Prokaryote - - // For interpretation of results, see: - // https://github.com/ncbi/fcs/wiki/FCS-adaptor#rules-for-action-assignment - // - // If adaptors are found, the pipeline stops with a report of adaptor contamination - } + // TODO nf-core: Specify your pipeline's command line flags + // Input options + input = null + // References + genome = null + igenomes_base = 's3://ngi-igenomes/igenomes/' + igenomes_ignore = false + + + // MultiQC options + multiqc_config = null + multiqc_title = null + multiqc_logo = null + max_multiqc_email_size = '25.MB' + multiqc_methods_description = null + + // Boilerplate options + outdir = null + publish_dir_mode = 'copy' + email = null + email_on_fail = null + plaintext_email = false + monochrome_logs = false + hook_url = null + help = false + version = false + + // Config options + config_profile_name = null + config_profile_description = null + custom_config_version = 'master' + custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" + config_profile_contact = null + config_profile_url = null + + + // Max resource options + // Defaults only, expecting to be overwritten + max_memory = '128.GB' + max_cpus = 16 + max_time = '240.h' + + // Schema validation default options + validationFailUnrecognisedParams = false + validationLenientMode = false + validationSchemaIgnoreParams = 'genomes,igenomes_base' + validationShowHiddenParams = false + validate_params = true - ncbi_fcs_gx { - // This is a very slow module. Skip it unless you really need it. - skip = 0 +} - // Get tax ID from https://www.ncbi.nlm.nih.gov/taxonomy - // Example assembly is from Neonectria ditissima - tax_id = "78410" +// Load base.config by default for all pipelines +includeConfig 'conf/base.config' - // NCBI FCS GX DB path - // Due to enormity of the DB size, the pipeline does NOT download the data. - // It must be setup by the user manually before running the pipeline. - // See instructions for DB setup: https://github.com/ncbi/fcs/wiki/FCS-GX - db_path = "/workspace/ComparativeDataSources/NCBI/FCS/GX/r2023-01-24" +// Load nf-core custom profiles from different Institutions +try { + includeConfig "${params.custom_config_base}/nfcore_custom.config" +} catch (Exception e) { + System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") +} - // For interpretation of results, see: - // https://github.com/ncbi/fcs/wiki/FCS-GX#fcs-gx-report-output +// Load plant-food-research-open/assemblyqc custom profiles from different institutions. +// Warning: Uncomment only if a pipeline-specific institutional config already exists on nf-core/configs! +// try { +// includeConfig "${params.custom_config_base}/pipeline/assemblyqc.config" +// } catch (Exception e) { +// System.err.println("WARNING: Could not load nf-core/config/assemblyqc profiles: ${params.custom_config_base}/pipeline/assemblyqc.config") +// } +profiles { + debug { + dumpHashes = true + process.beforeScript = 'echo $HOSTNAME' + cleanup = false + nextflow.enable.configProcessNamesValidation = true } - - busco { - skip = 0 - - // geno or genome, for genome assemblies (DNA) - // tran or transcriptome, for transcriptome assemblies (DNA) - // prot or proteins, for annotated gene sets (protein) - mode = "geno" - - // BUSCO runs for each assembly in combination with each lineage - // Full cartesian product: Assembly x Lineage - // - // To select a lineage, see https://busco.ezlab.org/list_of_lineages.html - lineage_datasets = ["fungi_odb10", "microsporidia_odb10"] - - // BUSCO DB download path - // The pipeline automatically downloads the required DB if needed - download_path = "/workspace/ComparativeDataSources/BUSCO/assembly_qc" + conda { + conda.enabled = true + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + channels = ['conda-forge', 'bioconda', 'defaults'] + apptainer.enabled = false } - - tidk { - skip = 0 - - // To select an appropriate sequence, see http://telomerase.asu.edu/sequences_telomere.html - // Plant: TTTAGGG, Fungus, Vertebrates: TTAGGG, Insect: TTAGG - repeat_seq = "TTAGGG" - - // 1: Filter assembly sequences by size, 0: Include all assembly sequences in the TIDK plot - filter_by_size = 0 - - // Filter size in base-pairs. Any sequence smaller than this size is filtered if filter_by_size=1 - // Default: 1000000 (1Mbp) - filter_size_bp = 1000000 + mamba { + conda.enabled = true + conda.useMamba = true + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false } - - lai { - skip = 0 - - // In calculation of LAI, this module assumes that each assembly specified in - // the target_assemblies is monoploid (1x) or only contains monoploid sequences. - // If this is not the case, use the following parameter to supply a single column - // text file listing IDs of the monoploid sequences for a polyploid assembly included - // in target_assemblies. The pipeline will only uses these sequences to compute LAI. - // - // This parameter is optional and can be set to [] if not needed. - // - // Pattern: - // monoploid_seqs = [["tag", "file_path1"], ["tag2", "file_path2"]] - monoploid_seqs = [] + docker { + docker.enabled = true + conda.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false + docker.runOptions = '-u $(id -u):$(id -g)' } - - kraken2 { - skip = 0 - - // To select a DB, see https://benlangmead.github.io/aws-indexes/k2 - // Due to enormity of the DB size, the pipeline does NOT download the data. - // - // Recommended PlusPFP: archaea, viral, plasmid, human, UniVec_Core, protozoa, fungi & plant - db_path = "/workspace/ComparativeDataSources/kraken2db/k2_pluspfp_20230314" + arm { + docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' } - - hic { - skip = 0 - - // A relative or absolute path to paired reads: *_R{1,2}.(fastq|fq).gz - // OR SRA: SRR8238190 - paired_reads = "/input/genomic/fungal/Neonectria/Genome/20190506_CAGRF19591_CGYCF_HiC/PG_PETUNIA_HiC_CGYCF_CACTCA_L001_R{1,2}.fastq.gz" - - skip_fastp = 0 - skip_fastqc = 0 - - fastp_ext_args = '--qualified_quality_phred 20 --length_required 50' + singularity { + singularity.enabled = true + singularity.autoMounts = true + conda.enabled = false + docker.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false } - - synteny { - skip = 0 - - between_target_asm = 1 // 1: Create syntenic plots between each pair of target_assemblies, 0: Don't - - // Assembly sequence list - // A two column tab-delimited txt file listing fasta sequence ids (first column) and - // labels for the synteny plots (second column). - // - // Filter sequences: If a sequence is missing from this file, it is excluded from the synteny plot even if - // it is present in the fasta file. - // - // Note: The sequence labels (second column) must be unique across the synteny assemblies. - // Otherwise, the pipeline fails with an error. - // - // Example tab-delimited file contents are listed here: - // Nd324_chr_1 ND_1 - // Nd324_chr_2 ND_2 - // - // Pattern: [["tag", "path to assembly sequence list"]] - assembly_seq_list = [ - ["hap1", "/workspace/assembly_qc/test_data/default/test_data1.seq.list"], - ["hap2", "/workspace/assembly_qc/test_data/default/test_data2.seq.list"] - ] - - // A list of reference assemblies. - // Syntenic plots are created between each assembly from target_assemblies and xref_assemblies. - // - // Optional Can be set to [] if not needed such as xref_assemblies = [] - // In this case, the synteny is only performed between target assemblies if between_target_asm is 1. - // - // Pattern: [["tag", "fasta file (fasta, fasta.gz) path", "seq.list file path"]] - xref_assemblies = [ - ["GenomeA", "/workspace/assembly_qc/test_data/default/test_data3.fasta", "/workspace/assembly_qc/test_data/default/test_data3.seq.list"], - ["GenomeB", "/workspace/assembly_qc/test_data/default/test_data4.fasta.gz", "/workspace/assembly_qc/test_data/default/test_data4.seq.list"] - ] - - // 0: Only include alignment blocks with 1-to-1 mappings (dnadiff .1coords file) - // 1: Include alignment blocks with many-to-many mappings (dnadiff .mcoords file) - many_to_many_align = 0 - - max_gap = 1000000 // 1 Mbp; Alignments within this distance are bundled together - min_bundle_size = 1000 // 1 Kbp; After bundling, any bundle smaller than this size is filtered out - - // 1: Create a separate synteny plot for each contig of the target assembly versus all contigs of the reference assembly - // 0: Only plot synteny for all contigs of target assembly versus all contigs of reference assembly - plot_1_vs_all = 1 - - // 1: Synteny plot is colored by contig - // 0: Synteny plot is colored by the number of links in a bundle - color_by_contig = 1 + podman { + podman.enabled = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false + } + shifter { + shifter.enabled = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + podman.enabled = false + charliecloud.enabled = false + apptainer.enabled = false + } + charliecloud { + charliecloud.enabled = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + apptainer.enabled = false + } + apptainer { + apptainer.enabled = true + apptainer.autoMounts = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + } + gitpod { + executor.name = 'local' + executor.cpus = 4 + executor.memory = 8.GB } + test { includeConfig 'conf/test.config' } + test_full { includeConfig 'conf/test_full.config' } +} - outdir = "./results" +// Set default registry for Apptainer, Docker, Podman and Singularity independent of -profile +// Will not be used unless Apptainer / Docker / Podman / Singularity are enabled +// Set to your registry if you have a mirror of containers +apptainer.registry = 'quay.io' +docker.registry = 'quay.io' +podman.registry = 'quay.io' +singularity.registry = 'quay.io' + +// Nextflow plugins +plugins { + id 'nf-validation@1.1.3' // Validation of pipeline parameters and creation of an input channel from a sample sheet +} +// Load igenomes.config if required +if (!params.igenomes_ignore) { + includeConfig 'conf/igenomes.config' +} else { + params.genomes = [:] +} +// Export these variables to prevent local Python/R libraries from conflicting with those in the container +// The JULIA depot path has been adjusted to a fixed path `/usr/local/share/julia` that needs to be used for packages in the container. +// See https://apeltzer.github.io/post/03-julia-lang-nextflow/ for details on that. Once we have a common agreement on where to keep Julia packages, this is adjustable. + +env { + PYTHONNOUSERSITE = 1 + R_PROFILE_USER = "/.Rprofile" + R_ENVIRON_USER = "/.Renviron" + JULIA_DEPOT_PATH = "/usr/local/share/julia" +} - // The resources are capped at their following maximum values. - // The pipeline does not use additional resources even if - // the following values are increased. These values only set the - // upper bound. - // - // To truly increase the max resources above the following values, - // change the process classes defined in './conf/base.config'. - max_cpus = 12 +// Capture exit codes from upstream processes when piping +process.shell = ['/bin/bash', '-euo', 'pipefail'] - // NCBI_FCS_GX requires at least 512 GBs - max_memory = 512.GB +// Disable process selector warnings by default. Use debug profile to enable warnings. +nextflow.enable.configProcessNamesValidation = false - // SYNTENY::DNADIFF can take up to a week - // - // Target and Reference assemblies: 2.5 GB - // MUMMER .delta file: 1 GB - // Execution time: 134 hours - max_time = 7.days +def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') +timeline { + enabled = true + file = "${params.outdir}/pipeline_info/execution_timeline_${trace_timestamp}.html" } - -apptainer { - // This is where the pipeline looks for and stores the singularity - // containers. - cacheDir = "/workspace/assembly_qc/singularity" +report { + enabled = true + file = "${params.outdir}/pipeline_info/execution_report_${trace_timestamp}.html" +} +trace { + enabled = true + file = "${params.outdir}/pipeline_info/execution_trace_${trace_timestamp}.txt" +} +dag { + enabled = true + file = "${params.outdir}/pipeline_info/pipeline_dag_${trace_timestamp}.html" } manifest { - name = "AssemblyQC" - nextflowVersion = '!>=22.04.3' - version = '1.4' + name = 'plant-food-research-open/assemblyqc' + author = """Usman Rashid""" + homePage = 'https://github.com/plant-food-research-open/assemblyqc' + description = """AssemblyQC is a NextFlow pipeline which evaluates assembly quality with well-established tools and presents the results in a unified html report.""" + mainScript = 'main.nf' + nextflowVersion = '!>=23.04.0' + version = '1.4dev' + doi = '' } -includeConfig './conf/modules.config' -includeConfig './conf/reporting_defaults.config' +// Load modules.config for DSL2 module specific options +includeConfig 'conf/modules.config' + +// Function to ensure that resource requirements don't go beyond +// a maximum limit +def check_max(obj, type) { + if (type == 'memory') { + try { + if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1) + return params.max_memory as nextflow.util.MemoryUnit + else + return obj + } catch (all) { + println " ### ERROR ### Max memory '${params.max_memory}' is not valid! Using default value: $obj" + return obj + } + } else if (type == 'time') { + try { + if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1) + return params.max_time as nextflow.util.Duration + else + return obj + } catch (all) { + println " ### ERROR ### Max time '${params.max_time}' is not valid! Using default value: $obj" + return obj + } + } else if (type == 'cpus') { + try { + return Math.min( obj, params.max_cpus as int ) + } catch (all) { + println " ### ERROR ### Max cpus '${params.max_cpus}' is not valid! Using default value: $obj" + return obj + } + } +} diff --git a/nextflow_schema.json b/nextflow_schema.json new file mode 100644 index 00000000..1a13c92b --- /dev/null +++ b/nextflow_schema.json @@ -0,0 +1,288 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/master/nextflow_schema.json", + "title": "plant-food-research-open/assemblyqc pipeline parameters", + "description": "AssemblyQC is a NextFlow pipeline which evaluates assembly quality with well-established tools and presents the results in a unified html report.", + "type": "object", + "definitions": { + "input_output_options": { + "title": "Input/output options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define where the pipeline should find input data and save output data.", + "required": ["input", "outdir"], + "properties": { + "input": { + "type": "string", + "format": "file-path", + "exists": true, + "mimetype": "text/csv", + "pattern": "^\\S+\\.csv$", + "description": "Path to comma-separated file containing information about the samples in the experiment.", + "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row.", + "fa_icon": "fas fa-file-csv" + }, + "outdir": { + "type": "string", + "format": "directory-path", + "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", + "fa_icon": "fas fa-folder-open" + }, + "email": { + "type": "string", + "description": "Email address for completion summary.", + "fa_icon": "fas fa-envelope", + "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" + }, + "multiqc_title": { + "type": "string", + "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", + "fa_icon": "fas fa-file-signature" + } + } + }, + "reference_genome_options": { + "title": "Reference genome options", + "type": "object", + "fa_icon": "fas fa-dna", + "description": "Reference genome related files and options required for the workflow.", + "properties": { + "genome": { + "type": "string", + "description": "Name of iGenomes reference.", + "fa_icon": "fas fa-book", + "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details." + }, + "fasta": { + "type": "string", + "format": "file-path", + "exists": true, + "mimetype": "text/plain", + "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$", + "description": "Path to FASTA genome file.", + "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", + "fa_icon": "far fa-file-code" + }, + "igenomes_ignore": { + "type": "boolean", + "description": "Do not load the iGenomes reference config.", + "fa_icon": "fas fa-ban", + "hidden": true, + "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." + } + } + }, + "institutional_config_options": { + "title": "Institutional config options", + "type": "object", + "fa_icon": "fas fa-university", + "description": "Parameters used to describe centralised config profiles. These should not be edited.", + "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.", + "properties": { + "custom_config_version": { + "type": "string", + "description": "Git commit id for Institutional configs.", + "default": "master", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "custom_config_base": { + "type": "string", + "description": "Base directory for Institutional configs.", + "default": "https://raw.githubusercontent.com/nf-core/configs/master", + "hidden": true, + "help_text": "If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.", + "fa_icon": "fas fa-users-cog" + }, + "config_profile_name": { + "type": "string", + "description": "Institutional config name.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_description": { + "type": "string", + "description": "Institutional config description.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_contact": { + "type": "string", + "description": "Institutional config contact information.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_url": { + "type": "string", + "description": "Institutional config URL link.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + } + } + }, + "max_job_request_options": { + "title": "Max job request options", + "type": "object", + "fa_icon": "fab fa-acquisitions-incorporated", + "description": "Set the top limit for requested resources for any single job.", + "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", + "properties": { + "max_cpus": { + "type": "integer", + "description": "Maximum number of CPUs that can be requested for any single job.", + "default": 16, + "fa_icon": "fas fa-microchip", + "hidden": true, + "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" + }, + "max_memory": { + "type": "string", + "description": "Maximum amount of memory that can be requested for any single job.", + "default": "128.GB", + "fa_icon": "fas fa-memory", + "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", + "hidden": true, + "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" + }, + "max_time": { + "type": "string", + "description": "Maximum amount of time that can be requested for any single job.", + "default": "240.h", + "fa_icon": "far fa-clock", + "pattern": "^(\\d+\\.?\\s*(s|m|h|d|day)\\s*)+$", + "hidden": true, + "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" + } + } + }, + "generic_options": { + "title": "Generic options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "properties": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "version": { + "type": "boolean", + "description": "Display version and exit.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "publish_dir_mode": { + "type": "string", + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "hidden": true + }, + "email_on_fail": { + "type": "string", + "description": "Email address for completion summary, only when pipeline fails.", + "fa_icon": "fas fa-exclamation-triangle", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", + "help_text": "An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.", + "hidden": true + }, + "plaintext_email": { + "type": "boolean", + "description": "Send plain-text email instead of HTML.", + "fa_icon": "fas fa-remove-format", + "hidden": true + }, + "max_multiqc_email_size": { + "type": "string", + "description": "File size limit when attaching MultiQC reports to summary emails.", + "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", + "default": "25.MB", + "fa_icon": "fas fa-file-upload", + "hidden": true + }, + "monochrome_logs": { + "type": "boolean", + "description": "Do not use coloured log outputs.", + "fa_icon": "fas fa-palette", + "hidden": true + }, + "hook_url": { + "type": "string", + "description": "Incoming hook URL for messaging service", + "fa_icon": "fas fa-people-group", + "help_text": "Incoming hook URL for messaging service. Currently, MS Teams and Slack are supported.", + "hidden": true + }, + "multiqc_config": { + "type": "string", + "format": "file-path", + "description": "Custom config file to supply to MultiQC.", + "fa_icon": "fas fa-cog", + "hidden": true + }, + "multiqc_logo": { + "type": "string", + "description": "Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file", + "fa_icon": "fas fa-image", + "hidden": true + }, + "multiqc_methods_description": { + "type": "string", + "description": "Custom MultiQC yaml file containing HTML including a methods description.", + "fa_icon": "fas fa-cog" + }, + "validate_params": { + "type": "boolean", + "description": "Boolean whether to validate parameters against the schema at runtime", + "default": true, + "fa_icon": "fas fa-check-square", + "hidden": true + }, + "validationShowHiddenParams": { + "type": "boolean", + "fa_icon": "far fa-eye-slash", + "description": "Show all params when using `--help`", + "hidden": true, + "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." + }, + "validationFailUnrecognisedParams": { + "type": "boolean", + "fa_icon": "far fa-check-circle", + "description": "Validation of parameters fails when an unrecognised parameter is found.", + "hidden": true, + "help_text": "By default, when an unrecognised parameter is found, it returns a warinig." + }, + "validationLenientMode": { + "type": "boolean", + "fa_icon": "far fa-check-circle", + "description": "Validation of parameters in lenient more.", + "hidden": true, + "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)." + } + } + } + }, + "allOf": [ + { + "$ref": "#/definitions/input_output_options" + }, + { + "$ref": "#/definitions/reference_genome_options" + }, + { + "$ref": "#/definitions/institutional_config_options" + }, + { + "$ref": "#/definitions/max_job_request_options" + }, + { + "$ref": "#/definitions/generic_options" + } + ] +} diff --git a/pfr_assemblyqc b/pfr_assemblyqc deleted file mode 100644 index eb668f21..00000000 --- a/pfr_assemblyqc +++ /dev/null @@ -1,19 +0,0 @@ -#!/bin/bash -e - -#SBATCH --job-name ASM_QC -#SBATCH --time=7-00:00:00 -#SBATCH --nodes=1 -#SBATCH --ntasks=1 -#SBATCH --cpus-per-task=1 -#SBATCH --output pfr_assemblyqc.stdout -#SBATCH --error pfr_assemblyqc.stderr -#SBATCH --mem=4G - -ml unload perl -ml apptainer/1.1 -ml nextflow/23.04.4 - -export TMPDIR="/workspace/$USER/tmp" -export APPTAINER_BINDPATH="$APPTAINER_BINDPATH,$TMPDIR:$TMPDIR,$TMPDIR:/tmp" - -nextflow main.nf -profile pfr,apptainer -resume diff --git a/pyproject.toml b/pyproject.toml new file mode 100644 index 00000000..984c0910 --- /dev/null +++ b/pyproject.toml @@ -0,0 +1,13 @@ +# Config file for Python. Mostly used to configure linting of bin/check_samplesheet.py with Ruff. +# Should be kept the same as nf-core/tools to avoid fighting with template synchronisation. +[tool.ruff] +line-length = 120 +target-version = "py38" +select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] +cache-dir = "~/.cache/ruff" + +[tool.ruff.isort] +known-first-party = ["nf_core"] + +[tool.ruff.per-file-ignores] +"__init__.py" = ["E402", "F401"] diff --git a/subworkflows/local/busco.nf b/subworkflows/local/busco.nf deleted file mode 100644 index 30c0b006..00000000 --- a/subworkflows/local/busco.nf +++ /dev/null @@ -1,84 +0,0 @@ -workflow BUSCO { - take: - tuple_of_hap_file_lineage - - main: - // MODULE: RUN_BUSCO - RUN_BUSCO ( tuple_of_hap_file_lineage ) - - ch_busco_summaries = RUN_BUSCO.out.summary - | collect - - // MODULE: RUN_BUSCO - CREATE_PLOT ( ch_busco_summaries ) - - ch_busco_plot = CREATE_PLOT.out.png - - emit: - summary = RUN_BUSCO.out.summary - plot = ch_busco_plot - versions = Channel.empty().mix(RUN_BUSCO.out.versions.first()) -} - -process RUN_BUSCO { - tag "${hap_name}:${lineage_dataset}" - label "process_high" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/busco:5.2.2--pyhdfd78af_0': - 'quay.io/biocontainers/busco:5.2.2--pyhdfd78af_0' }" - - input: - tuple val(hap_name), path(fasta_file), val(lineage_dataset) - - output: - path "${hap_name}/short_summary.specific.${lineage_dataset}.${hap_name}_${lineage_split}.txt" , emit: summary - path "versions.yml" , emit: versions - - script: - def lineages_path = params.busco.download_path ? "--download_path ${params.busco.download_path}" : '' - def lineage_to_split = "${lineage_dataset}"; - def parts = lineage_to_split.split("_"); - lineage_split = parts[0]; - - """ - busco \ - -m ${params.busco.mode} \ - -o ${hap_name} \ - -i $fasta_file \ - -l ${lineage_dataset} \ - --update-data \ - $lineages_path \ - -c ${task.cpus} - - mv "${hap_name}/short_summary.specific.${lineage_dataset}.${hap_name}.txt" \\ - "${hap_name}/short_summary.specific.${lineage_dataset}.${hap_name}_${lineage_split}.txt" - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - busco: \$( busco --version 2>&1 | sed 's/^BUSCO //' ) - END_VERSIONS - """ -} - -process CREATE_PLOT { - tag "all summaries" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/busco:5.2.2--pyhdfd78af_0': - 'quay.io/biocontainers/busco:5.2.2--pyhdfd78af_0' }" - - publishDir params.outdir, mode: 'copy' - - input: - path "short_summary.*", stageAs: 'busco/*' - - output: - path 'busco/*.png', emit: png - - script: - """ - generate_plot.py -wd ./busco - """ -} diff --git a/subworkflows/local/create_hic_file.nf b/subworkflows/local/create_hic_file.nf deleted file mode 100644 index 8ce10049..00000000 --- a/subworkflows/local/create_hic_file.nf +++ /dev/null @@ -1,27 +0,0 @@ -include { MAKE_AGP_FROM_FASTA } from '../../modules/local/make_agp_from_fasta.nf' -include { AGP2_ASSEMBLY } from '../../modules/local/agp2_assembly.nf' -include { ASSEMBLY2_BEDPE } from '../../modules/local/assembly2_bedpe.nf' -include { MATLOCK_BAM2_JUICER } from '../../modules/local/matlock_bam2_juicer.nf' -include { JUICER_SORT } from '../../modules/local/juicer_sort.nf' -include { RUN_ASSEMBLY_VISUALIZER } from '../../modules/local/run_assembly_visualizer.nf' - -workflow CREATE_HIC_FILE { - take: - tuple_of_tag_fa_bam - - main: - // MODULE: MAKE_AGP_FROM_FASTA | AGP2_ASSEMBLY | ASSEMBLY2_BEDPE - MAKE_AGP_FROM_FASTA ( tuple_of_tag_fa_bam.map { tag, fa, bam -> [ tag, fa ] } ) - | AGP2_ASSEMBLY - | ASSEMBLY2_BEDPE - - // MODULE: MATLOCK_BAM2_JUICER | JUICER_SORT - MATLOCK_BAM2_JUICER ( tuple_of_tag_fa_bam.map { tag, fa, bam -> [ tag, bam ] } ) - | JUICER_SORT - - // MODULE: RUN_ASSEMBLY_VISUALIZER - RUN_ASSEMBLY_VISUALIZER ( AGP2_ASSEMBLY.out.assembly.join(JUICER_SORT.out.links) ) - - emit: - hic = RUN_ASSEMBLY_VISUALIZER.out.hic -} diff --git a/subworkflows/local/hic_contact_map.nf b/subworkflows/local/hic_contact_map.nf deleted file mode 100644 index e102a981..00000000 --- a/subworkflows/local/hic_contact_map.nf +++ /dev/null @@ -1,62 +0,0 @@ -include { FASTQ_BWA_MEM_SAMBLASTER } from '../pfr/fastq_bwa_mem_samblaster/main' -include { CREATE_HIC_FILE } from './create_hic_file.nf' -include { HIC_QC } from '../../modules/local/hic_qc.nf' - -workflow HIC_CONTACT_MAP { - take: - reads // [ val(meta), [ fq ] ] - fasta // [ val(tag), fasta ] - - main: - - // SUBWORKFLOW: FASTQ_BWA_MEM_SAMBLASTER - FASTQ_BWA_MEM_SAMBLASTER( - reads, - fasta.map { tag, fasta -> [ [ id: tag ], fasta, [] ] } - ) - - ch_bam = FASTQ_BWA_MEM_SAMBLASTER.out.bam - - ch_fasta_and_bam = ch_bam - | map { meta, bam -> [ meta.ref_id, meta, bam ] } - | join( - fasta - ) - | map { ref_id, meta, bam, fasta -> - [ "${meta.id}.on.${meta.ref_id}", fasta, bam ] - } - - // MODULE: HIC_QC - HIC_QC ( ch_fasta_and_bam.map { id, fasta, bam -> [ id, bam ] } ) - - // SUBWORKFLOW: CREATE_HIC_FILE | MODULE: HIC2_HTML - CREATE_HIC_FILE ( ch_fasta_and_bam ) - | HIC2_HTML - - ch_versions = Channel.empty() - | mix(FASTQ_BWA_MEM_SAMBLASTER.out.versions) - - emit: - html = HIC2_HTML.out.html - versions = ch_versions -} - -process HIC2_HTML { - tag "$sample_id_on_tag" - label "process_single" - - container "docker.io/gallvp/python3npkgs:v0.4" - publishDir "${params.outdir}/hic", mode: 'copy' - - input: - tuple val(sample_id_on_tag), path(hic_file) - - output: - path "*.html", emit: html - - script: - """ - file_name="$hic_file" - hic_2_html_fc62f04.py "$hic_file" > "\${file_name%.*}.html" - """ -} diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf new file mode 100644 index 00000000..0aecf87f --- /dev/null +++ b/subworkflows/local/input_check.nf @@ -0,0 +1,44 @@ +// +// Check input samplesheet and get read channels +// + +include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' + +workflow INPUT_CHECK { + take: + samplesheet // file: /path/to/samplesheet.csv + + main: + SAMPLESHEET_CHECK ( samplesheet ) + .csv + .splitCsv ( header:true, sep:',' ) + .map { create_fastq_channel(it) } + .set { reads } + + emit: + reads // channel: [ val(meta), [ reads ] ] + versions = SAMPLESHEET_CHECK.out.versions // channel: [ versions.yml ] +} + +// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] +def create_fastq_channel(LinkedHashMap row) { + // create meta map + def meta = [:] + meta.id = row.sample + meta.single_end = row.single_end.toBoolean() + + // add path(s) of the fastq file(s) to the meta map + def fastq_meta = [] + if (!file(row.fastq_1).exists()) { + exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" + } + if (meta.single_end) { + fastq_meta = [ meta, [ file(row.fastq_1) ] ] + } else { + if (!file(row.fastq_2).exists()) { + exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" + } + fastq_meta = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] + } + return fastq_meta +} diff --git a/subworkflows/local/kraken2.nf b/subworkflows/local/kraken2.nf deleted file mode 100644 index 2d3bcb3c..00000000 --- a/subworkflows/local/kraken2.nf +++ /dev/null @@ -1,102 +0,0 @@ -include { UNTAR } from '../../modules/nf-core/untar/main.nf' - -workflow KRAKEN2 { - take: - tuple_of_hap_file - db_path // channel: path - - main: - ch_tar_db = db_path - | filter { db -> "$db".endsWith('.tar.gz') } - - ch_untar_db = db_path - | filter { db -> !( "$db".endsWith('.tar.gz') ) } - - // MODULE: UNTAR - UNTAR ( ch_tar_db.map { tar -> [ [ id: "kraken2_db" ], tar ] } ) - - ch_kraken2_inputs = UNTAR.out.untar - | map { meta, untar -> untar } - | mix( - ch_untar_db - ) - | combine(tuple_of_hap_file) - - // MODULE: RUN_KRAKEN2 - RUN_KRAKEN2( - ch_kraken2_inputs.map { db, tag, fasta -> [ tag, fasta ] }, - ch_kraken2_inputs.map { db, tag, fasta -> db } - ) - - // MODULE: KRONA_PLOT - KRONA_PLOT ( RUN_KRAKEN2.out.report ) - - ch_versions = Channel.empty() - | mix(RUN_KRAKEN2.out.versions.first()) - | mix(UNTAR.out.versions.first()) - | mix(KRONA_PLOT.out.versions.first()) - - emit: - plot = KRONA_PLOT.out.plot - versions = ch_versions -} - -process RUN_KRAKEN2 { - tag "${hap_name}" - label "process_single" - label "process_high_memory" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/kraken2:2.1.2--pl5321h9f5acd7_2': - 'quay.io/biocontainers/kraken2:2.1.2--pl5321h9f5acd7_2' }" - - input: - tuple val(hap_name), path(fasta_file) - path db_path - - output: - tuple val(hap_name), path("*.kraken2.cut"), path("*.kraken2.report"), emit: report - path "versions.yml" , emit: versions - - script: - """ - kraken2 \\ - --output "${hap_name}.kraken2.cut" \\ - --report "${hap_name}.kraken2.report" \\ - --use-names \\ - --db $db_path \\ - --threads ${task.cpus} \\ - $fasta_file > kraken2.log - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - kraken2: \$(echo \$(kraken2 --version 2>&1) | sed 's/^.*Kraken version //; s/ .*\$//') - END_VERSIONS - """ -} - -process KRONA_PLOT { - tag "${hap_name}" - label "process_single" - - container "docker.io/nanozoo/krona:2.7.1--e7615f7" - publishDir "${params.outdir}/kraken2", mode: 'copy' - - input: - tuple val(hap_name), path(kraken2_cut), path(kraken2_report) - - output: - tuple path("*.kraken2.krona.cut"), path("*.kraken2.krona.html"), emit: plot - path "versions.yml" , emit: versions - - script: - """ - perl -lane '@a=split /\\t/; if (\$a[2] =~ /taxid\\s+(\\d+)/) {print "\$a[1]\\t\$1\\t1\\t\$a[3]";}' $kraken2_cut > "${hap_name}.kraken2.krona.cut" - ktImportTaxonomy -i -o "${hap_name}.kraken2.krona.html" -m "4" "${hap_name}.kraken2.krona.cut" - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - KronaTools: \$(ktImportTaxonomy | sed -n '/KronaTools/s/KronaTools//p' | tr -d ' _/[:space:]' | sed 's/-ktImportTaxonomy\\\\//1') - END_VERSIONS - """ -} diff --git a/subworkflows/local/ncbi_fcs_gx.nf b/subworkflows/local/ncbi_fcs_gx.nf deleted file mode 100644 index e114968f..00000000 --- a/subworkflows/local/ncbi_fcs_gx.nf +++ /dev/null @@ -1,142 +0,0 @@ -workflow NCBI_FCS_GX { - take: - tuple_of_tag_file - db_path // channel: path - - main: - // MODULE: SETUP_SAMPLE - SETUP_SAMPLE ( tuple_of_tag_file ) - - ch_all_samples = SETUP_SAMPLE.out.fsata - | collect - - // MODULE: NCBI_FCS_GX_SCREEN_SAMPLES - NCBI_FCS_GX_SCREEN_SAMPLES ( ch_all_samples, db_path ) - - ch_gx_report = NCBI_FCS_GX_SCREEN_SAMPLES.out.fcs_gx_reports - | flatten - | map { - def parts = it.getName().split("\\.") - def tag = parts[0] - [tag, it] - } - - ch_gx_taxonomy = NCBI_FCS_GX_SCREEN_SAMPLES.out.fcs_gx_taxonomies - | flatten - | map { - def parts = it.getName().split("\\.") - def tag = parts[0] - [tag, it] - } - - // MODULE: FCS_GX_KRONA_PLOT - FCS_GX_KRONA_PLOT ( ch_gx_taxonomy ) - - ch_gx_taxonomy_plot = FCS_GX_KRONA_PLOT.out.plot - - ch_versions = Channel.empty() - | mix(NCBI_FCS_GX_SCREEN_SAMPLES.out.versions.first()) - | mix(FCS_GX_KRONA_PLOT.out.versions.first()) - - emit: - gx_report = ch_gx_report - gx_taxonomy = ch_gx_taxonomy - gx_taxonomy_plot = ch_gx_taxonomy_plot - versions = ch_versions -} - -process SETUP_SAMPLE { - tag "${hap_name}" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/ubuntu:20.04': - 'quay.io/nf-core/ubuntu:20.04' }" - - input: - tuple val(hap_name), path(fasta_file) - - output: - path 'fasta.file.for.*.fasta', emit: fsata - - script: - """ - ln -s $fasta_file "fasta.file.for.${hap_name}.fasta" - """ -} - - -process NCBI_FCS_GX_SCREEN_SAMPLES { - tag "all samples" - label "process_high" - label "process_long" - label "process_very_high_memory" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://ftp.ncbi.nlm.nih.gov/genomes/TOOLS/FCS/releases/0.4.0/fcs-gx.sif': - 'docker.io/ncbi/fcs-gx:0.4.0' }" - - input: - path samples - path db_path - - output: - path "*.fcs_gx_report.txt" , emit: fcs_gx_reports - path "*.taxonomy.rpt" , emit: fcs_gx_taxonomies - path "versions.yml" , emit: versions - - script: - def VERSION = 0.4 - """ - for sample_fasta in $samples; - do - sample_tag=\$(echo "\$sample_fasta" | sed 's/fasta.file.for.//g' | sed 's/.fasta//g') - python3 /app/bin/run_gx --fasta ./\$sample_fasta --out-dir ./ --gx-db $db_path --tax-id "${params.ncbi_fcs_gx.tax_id}" - - mv "\${sample_fasta%.fasta}.${params.ncbi_fcs_gx.tax_id}.fcs_gx_report.txt" "\${sample_tag}.fcs_gx_report.txt" - mv "\${sample_fasta%.fasta}.${params.ncbi_fcs_gx.tax_id}.taxonomy.rpt" "\${sample_tag}.taxonomy.rpt" - done - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - fcs_gx: $VERSION - END_VERSIONS - """ -} - -process FCS_GX_KRONA_PLOT { - tag "${tag_name}" - label "process_single" - - container "docker.io/nanozoo/krona:2.7.1--e7615f7" - publishDir "${params.outdir}/ncbi_fcs_gx", mode: 'copy' - - input: - tuple val(tag_name), path(fcs_gx_taxonomy) - - output: - tuple path("${tag_name}.inter.tax.rpt.tsv"), path("${tag_name}.fcs.gx.krona.cut"), path("${tag_name}.fcs.gx.krona.html") , emit: plot - path "versions.yml" , emit: versions - - script: - """ - cat $fcs_gx_taxonomy \\ - | awk 'NR>1 {print \$1,\$2,\$6,\$7,\$11,\$32}' FS="\\t" OFS="\\t" \\ - > "${tag_name}.inter.tax.rpt.tsv" - - cat "${tag_name}.inter.tax.rpt.tsv" \\ - | awk '\$6 !~ /(bogus|repeat|low-coverage|inconclusive)/ {print \$1,\$4,\$5,\$2}' FS="\\t" OFS="\\t" \\ - > "${tag_name}.fcs.gx.krona.cut" - - cat "${tag_name}.inter.tax.rpt.tsv" \\ - | awk 'NR>1 && \$6 ~ /(bogus|repeat|low-coverage|inconclusive)/ {print \$1,"0",\$5,\$2}' FS="\\t" OFS="\\t" \\ - >> "${tag_name}.fcs.gx.krona.cut" - - ktImportTaxonomy -i -o "${tag_name}.fcs.gx.krona.html" -m "4" "${tag_name}.fcs.gx.krona.cut" - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - KronaTools: \$(ktImportTaxonomy | sed -n '/KronaTools/s/KronaTools//p' | tr -d ' _/[:space:]' | sed 's/-ktImportTaxonomy\\\\//1') - END_VERSIONS - """ -} diff --git a/subworkflows/local/synteny.nf b/subworkflows/local/synteny.nf deleted file mode 100644 index f4fa5b59..00000000 --- a/subworkflows/local/synteny.nf +++ /dev/null @@ -1,638 +0,0 @@ -include { GUNZIP as GUNZIP_FASTA } from '../../modules/nf-core/gunzip/main' - -workflow SYNTENY { - take: - tuple_of_tag_fasta_seq_list - tuple_of_tag_xref_fasta_seq_list - - main: - ch_between_target_asm_combinations = !params.synteny.between_target_asm - ? Channel.empty() - : tuple_of_tag_fasta_seq_list - | map { [it] } - | collect - | map { getUniqueWithinCombinations(it) } - | flatten - | buffer(size:6) - - ch_xref_fa_branch = tuple_of_tag_xref_fasta_seq_list - | map { tag, fa, seq_list -> - [ [ id: tag ], fa ] - } - | branch { meta, fa -> - gz: "$fa".endsWith(".gz") - rest: !"$fa".endsWith(".gz") - } - - // MODULE: GUNZIP_FASTA - GUNZIP_FASTA ( ch_xref_fa_branch.gz ) - - ch_xref_ungz_fasta_seq_list = GUNZIP_FASTA.out.gunzip - | mix( - ch_xref_fa_branch.rest - ) - | map { meta, fa -> [ meta.id, fa ] } - | join( - tuple_of_tag_xref_fasta_seq_list - ) - | map { tag, fa, input_fa, seq_list -> - [ tag, fa, seq_list ] - } - - // MODULE: FILTER_SORT_FASTA_AND_VALIDATE_SEQ_LISTS | MUMMER & GET_FASTA_LEN - ch_seq_lists = ch_between_target_asm_combinations - .mix( - tuple_of_tag_fasta_seq_list - | combine( - ch_xref_ungz_fasta_seq_list - ) - ) - .tap { ch_full_tap_from_all_combinations } - | map { target_tag, target_fa, target_sl, xref_tag, xref_fa, xref_sl -> - [ "${target_tag}.on.${xref_tag}", target_sl, xref_sl ] - } - - FILTER_SORT_FASTA_AND_VALIDATE_SEQ_LISTS ( ch_full_tap_from_all_combinations ) - | ( MUMMER & GET_FASTA_LEN ) - - - ch_dnadiff_inputs = FILTER_SORT_FASTA_AND_VALIDATE_SEQ_LISTS.out.tags_fasta_files - | map { target, reference, target_fasta, ref_fasta -> - [ "${target}.on.${reference}", target_fasta, ref_fasta ] - } - | join( - MUMMER.out.tag_delta_file - ) - - // MODULE: DNADIFF | CIRCOS_BUNDLE_LINKS | ADD_COLOUR_TO_BUNDLE_LINKS - DNADIFF ( ch_dnadiff_inputs ) - | CIRCOS_BUNDLE_LINKS - | ADD_COLOUR_TO_BUNDLE_LINKS - - ch_coloured_links = ADD_COLOUR_TO_BUNDLE_LINKS.out.coloured_bundle_links - - ch_relabel_links_inputs = ch_coloured_links - | join(ch_seq_lists) - - // MODULE: RELABEL_BUNDLE_LINKS | SPLIT_BUNDLE_FILE_BY_TARGET_SEQS - RELABEL_BUNDLE_LINKS ( ch_relabel_links_inputs ) - | SPLIT_BUNDLE_FILE_BY_TARGET_SEQS - - ch_circos_split_bundle_links = SPLIT_BUNDLE_FILE_BY_TARGET_SEQS.out.split_file - | map { flattenSplitBundles(it) } - | flatten - | buffer(size:3) - - // MODULE: RELABEL_FASTA_LEN - ch_relabel_fasta_len_inputs = GET_FASTA_LEN.out.tag_len_files - | join(ch_seq_lists) - - RELABEL_FASTA_LEN ( ch_relabel_fasta_len_inputs ) - - // MODULE: GENERATE_KARYOTYPE - ch_generate_karyotype_inputs = RELABEL_FASTA_LEN.out.relabeled_seq_lengths - | cross( - ch_circos_split_bundle_links - ) - | map { seq_len_tuple, split_bundle_tuple -> - - def target_on_xref = seq_len_tuple[0] - def seq_tag = split_bundle_tuple[1] - def split_bundle_file = split_bundle_tuple[2] - def target_seq_len = seq_len_tuple[1] - def ref_seq_len = seq_len_tuple[2] - - [ target_on_xref, seq_tag, split_bundle_file, target_seq_len, ref_seq_len ] - } - GENERATE_KARYOTYPE ( ch_generate_karyotype_inputs ) - - // MODULE: CIRCOS - ch_circos_inputs = GENERATE_KARYOTYPE.out.karyotype - | join( - ch_circos_split_bundle_links - | map { target_on_xref, seq_tag, txt -> - [ "${target_on_xref}.${seq_tag}", txt ] - } - ) - CIRCOS ( ch_circos_inputs ) - - ch_versions = Channel.empty() - | mix(MUMMER.out.versions.first()) - | mix(CIRCOS.out.versions.first()) - - emit: - plot = CIRCOS.out.png_file - versions = ch_versions -} - -def getUniqueWithinCombinations(inputArray) { - if (inputArray.size() <= 1) { - return [] - } - - inputArray.sort { a, b -> a[0].compareTo(b[0]) } - - def outputList = [] - - for (int i = 0; i < inputArray.size() - 1; i++) { - for (int j = i + 1; j < inputArray.size(); j++) { - def combination = [ - inputArray[i][0], - file(inputArray[i][1], checkIfExists: true), - file(inputArray[i][2], checkIfExists: true), - inputArray[j][0], - file(inputArray[j][1], checkIfExists: true), - file(inputArray[j][2], checkIfExists: true) - ] - outputList.add(combination) - } - } - return outputList -} - -def appendTags(tag, valuesArray) { - if (valuesArray.size() <= 1) { - return [] - } - - def outputList = [] - - for (int i = 0; i < valuesArray.size(); i++) { - outputList.add([tag, valuesArray[i]]) - } - return outputList -} - -def flattenSplitBundles(inputArray) { - def target_on_ref = inputArray[0] - def files = inputArray[1] - - if(files in ArrayList) { - return files.collect { [target_on_ref, extractBundleTag(it), it] } - } else { - return [files].collect { [target_on_ref, extractBundleTag(it), it] } - } -} - -def extractBundleTag(filePath) { - def regex = /.*\.(\w+)\.split\.bundle\.txt/ - def matcher = filePath =~ regex - if (matcher.matches()) { - return matcher.group(1) - } else { - // This branch should never be executed if all the upstream logic is implemented correctly. - error "Error: Failed to parse the sequence tag from file name: ${filePath.getName()}" - } -} - -process FILTER_SORT_FASTA_AND_VALIDATE_SEQ_LISTS { - tag "${target}.on.${reference}" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1': - 'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }" - - input: - tuple val(target), path(target_fasta), path(target_seq_list), val(reference), path(ref_fasta), path(ref_seq_list) - - output: - tuple val(target), val(reference), path("filtered.ordered.target.fasta"), path("filtered.ordered.ref.fasta"), emit: tags_fasta_files - - script: - """ - validate_seq_lists_1d50376.sh "$target_seq_list" "$ref_seq_list" - samtools faidx $target_fasta \$(awk '{print \$1}' $target_seq_list) > filtered.ordered.target.fasta - samtools faidx $ref_fasta \$(awk '{print \$1}' $ref_seq_list) > filtered.ordered.ref.fasta - """ -} - -process GET_FASTA_LEN { - tag "${target}.on.${reference}" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1': - 'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }" - - input: - tuple val(target), val(reference), path(filtered_ordered_target_fasta), path(filtered_ordered_ref_fasta) - - output: - tuple val("${target}.on.${reference}"), path("target.seq.lengths"), path("ref.seq.lengths"), emit: tag_len_files - - script: - """ - samtools faidx $filtered_ordered_target_fasta - samtools faidx $filtered_ordered_ref_fasta - - cat "${filtered_ordered_target_fasta}.fai" | awk '{print \$1, \$2}' OFS="\\t" > target.seq.lengths - cat "${filtered_ordered_ref_fasta}.fai" | awk '{print \$1, \$2}' OFS="\\t" > ref.seq.lengths - """ -} - -process MUMMER { - tag "${target}.on.${reference}" - label "process_high" - - container "docker.io/staphb/mummer:4.0.0" - - input: - tuple val(target), val(reference), path(target_fasta), path(ref_fasta) - - output: - tuple val("${target}.on.${reference}"), path("*.delta"), emit: tag_delta_file - path "versions.yml", emit: versions - - script: - """ - nucmer \ - --mum \\ - -t ${task.cpus} \\ - -p "${target}.on.${reference}" \\ - $ref_fasta \\ - $target_fasta - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - nucmer: \$(nucmer -V) - END_VERSIONS - """ -} - -process DNADIFF { - tag "${target_on_ref}" - label "process_single" - label "process_week_long" - - container "docker.io/staphb/mummer:4.0.0" - - input: - tuple val(target_on_ref), path(target_fasta), path(ref_fasta), path(dnadiff_file) - - output: - tuple val(target_on_ref), path("*.xcoords"), path("*.report") - - script: - def inter_extension = params.synteny.many_to_many_align == 1 ? 'mcoords' : '1coords' - def out_extension = params.synteny.many_to_many_align == 1 ? 'm.xcoords' : '1.xcoords' - """ - cat \\ - $dnadiff_file \\ - | sed '1s/.*/${ref_fasta} ${target_fasta}/' \\ - > ${target_on_ref}.sed.delta - - dnadiff \\ - -p $target_on_ref \\ - -d ${target_on_ref}.sed.delta - - cat \\ - "${target_on_ref}.${inter_extension}" \\ - > "${target_on_ref}.${out_extension}" - """ -} - -process CIRCOS_BUNDLE_LINKS { - tag "${target_on_ref}" - label "process_single" - - container "docker.io/gallvp/circos-tools:v0.23-1_ps" - - input: - tuple val(target_on_ref), path(coords_file), path(report_file) - - output: - tuple val(target_on_ref), path("*.xcoords.bundle.txt") - - script: - """ - cat $coords_file | awk '{print \$12,\$1,\$2,\$13,\$3,\$4}' OFS="\\t" > "\$(basename $coords_file).links.txt" - - /usr/share/circos/tools/bundlelinks/bin/bundlelinks \\ - -links "\$(basename $coords_file).links.txt" \\ - -max_gap "${params.synteny.max_gap}" \\ - -min_bundle_size "${params.synteny.min_bundle_size}" \\ - 1>"\$(basename $coords_file).bundle.txt" \\ - 2>bundlelinks.err - """ -} - -process ADD_COLOUR_TO_BUNDLE_LINKS { - tag "${target_on_ref}" - label "process_single" - - container "docker.io/gallvp/python3npkgs:v0.4" - - input: - tuple val(target_on_ref), path(bundle_links) - - output: - tuple val(target_on_ref), path("*.xcoords.bundle.coloured.txt"), emit: coloured_bundle_links - - script: - """ - if [[ "${params.synteny.color_by_contig}" = "1" ]];then - color_circos_bundles_by_contig_943e0fb.py \\ - "${bundle_links}" \\ - > "\$(basename $bundle_links .bundle.txt).bundle.coloured.txt" - else - add_color_2_circos_bundle_file_943e0fb.pl \\ - -i="${bundle_links}" \\ - -o="\$(basename $bundle_links .bundle.txt).bundle.coloured.txt" - fi - """ -} - -process RELABEL_BUNDLE_LINKS { - tag "${target_on_ref}" - label "process_single" - - container "docker.io/gallvp/python3npkgs:v0.4" - - input: - tuple val(target_on_ref), path(coloured_bundle_links), path(target_seq_list), path(ref_seq_list) - - output: - tuple val(target_on_ref), path("*.xcoords.bundle.coloured.relabeled.txt"), emit: relabeled_coloured_bundle_links - - script: - """ - #!/usr/bin/env python - - import pandas as pd - import sys - import os - - output_file_name = ".".join("$coloured_bundle_links".split(".")[0:-1]) + ".relabeled.txt" - - subs_target_seq = pd.read_csv('$target_seq_list', sep='\\t', header=None) - subs_target_seq_dict = dict(zip(subs_target_seq.iloc[:, 0], subs_target_seq.iloc[:, 1])) - - subs_ref_seq = pd.read_csv('$ref_seq_list', sep='\\t', header=None) - subs_ref_seq_dict = dict(zip(subs_ref_seq.iloc[:, 0], subs_ref_seq.iloc[:, 1])) - - if os.path.getsize('$coloured_bundle_links') == 0: - with open(output_file_name, 'w') as f: - f.write('') - sys.exit(0) - else: - df = pd.read_csv('$coloured_bundle_links', sep=' ', header=None) - - df.iloc[:, 3] = df.iloc[:, 3].replace(subs_target_seq_dict, regex=False) - df.iloc[:, 0] = df.iloc[:, 0].replace(subs_ref_seq_dict, regex=False) - - df.to_csv(output_file_name, sep=' ', index=False, header=None) - """ -} - -process RELABEL_FASTA_LEN { - tag "${target_on_ref}" - label "process_single" - - container "docker.io/gallvp/python3npkgs:v0.4" - - input: - tuple val(target_on_ref), path(target_seq_lengths), path(ref_seq_lengths), path(target_seq_list), path(ref_seq_list) - - output: - tuple val(target_on_ref), path("relabeld.target.seq.lengths"), path("relabeld.ref.seq.lengths"), emit: relabeled_seq_lengths - - script: - """ - #!/usr/bin/env python - - import pandas as pd - - subs_target_seq = pd.read_csv('$target_seq_list', sep='\\t', header=None) - subs_target_seq_dict = dict(zip(subs_target_seq.iloc[:, 0], subs_target_seq.iloc[:, 1])) - - subs_ref_seq = pd.read_csv('$ref_seq_list', sep='\\t', header=None) - subs_ref_seq_dict = dict(zip(subs_ref_seq.iloc[:, 0], subs_ref_seq.iloc[:, 1])) - - df_target_seq_lengths = pd.read_csv('$target_seq_lengths', sep='\\t', header=None) - df_target_seq_lengths.iloc[:, 0] = df_target_seq_lengths.iloc[:, 0].replace(subs_target_seq_dict, regex=False) - df_target_seq_lengths.to_csv("relabeld.target.seq.lengths", sep='\\t', index=False, header=None) - - df_ref_seq_lengths = pd.read_csv('$ref_seq_lengths', sep='\\t', header=None) - df_ref_seq_lengths.iloc[:, 0] = df_ref_seq_lengths.iloc[:, 0].replace(subs_ref_seq_dict, regex=False) - df_ref_seq_lengths.to_csv("relabeld.ref.seq.lengths", sep='\\t', index=False, header=None) - """ -} - -process SPLIT_BUNDLE_FILE_BY_TARGET_SEQS { - tag "${target_on_ref}" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/ubuntu:20.04': - 'quay.io/nf-core/ubuntu:20.04' }" - - input: - tuple val(target_on_ref), path(coloured_bundle_links) - - output: - tuple val(target_on_ref), path("*.split.bundle.txt"), emit: split_file - - script: - """ - if [[ "${params.synteny.plot_1_vs_all}" = "1" ]];then - target_seqs=(\$(awk '{print \$4}' $coloured_bundle_links | sort | uniq)) - - for i in "\${!target_seqs[@]}" - do - target_seq=\${target_seqs[\$i]} - awk -v seq="\$target_seq" '\$4==seq {print \$0}' $coloured_bundle_links > "${target_on_ref}.\${target_seq}.split.bundle.txt" - done - fi - - cat $coloured_bundle_links > "${target_on_ref}.all.split.bundle.txt" - """ -} - -process GENERATE_KARYOTYPE { - tag "${target_on_ref}.${seq_tag}" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? - 'https://depot.galaxyproject.org/singularity/ubuntu:20.04': - 'quay.io/nf-core/ubuntu:20.04' }" - - input: - tuple val(target_on_ref), val(seq_tag), path(split_bundle_file), path(target_seq_len), path(ref_seq_len) - - output: - tuple val("${target_on_ref}.${seq_tag}"), path("*.karyotype"), emit: karyotype - - script: - """ - ref_seqs=(\$(awk '{print \$1}' $split_bundle_file | sort | uniq)) - - if [ \${#ref_seqs[@]} -eq 0 ]; then - touch "${target_on_ref}.${seq_tag}.karyotype" - exit 0 - fi - - tmp_file=\$(mktemp) - printf '%s\\n' "\${ref_seqs[@]}" > "\$tmp_file" - - if [[ $seq_tag = "all" ]];then - cat $target_seq_len > filtered.target.seq.len - else - grep -w "$seq_tag" $target_seq_len > filtered.target.seq.len - fi - cat filtered.target.seq.len | awk '{print \$1,\$2,"grey"}' OFS="\\t" > colored.filtered.target.seq.len - - grep -w -f "\$tmp_file" $ref_seq_len > filtered.ref.seq.len - cat filtered.ref.seq.len | awk '{print \$1,\$2,"black"}' OFS="\\t" > colored.filtered.ref.seq.len - - cat colored.filtered.ref.seq.len | sort -k1V > merged.seq.lengths - cat colored.filtered.target.seq.len | sort -k1Vr >> merged.seq.lengths - sed -i '/^\$/d' merged.seq.lengths - - cat merged.seq.lengths \ - | awk '{print "chr -",\$1,\$1,"0",\$2-1,\$3}' OFS="\\t" \ - > "${target_on_ref}.${seq_tag}.karyotype" - - rm "\$tmp_file" - """ -} - -process CIRCOS { - tag "${target_on_ref_seq}" - label "process_single" - - container "docker.io/gallvp/circos-tools:v0.23-1_ps" - - input: - tuple val(target_on_ref_seq), path(karyotype), path(bundle_file) - - output: - path "*.svg", emit: svg_file - path "*.png", emit: png_file - path "bundled.links.tsv", emit: bundled_links_tsv - path "circos.conf", emit: circos_conf - path "karyotype.tsv", emit: karyotype_tsv - path "versions.yml", emit: versions - - script: - """ - - links_count=\$(wc -l < "$bundle_file") - max_links=20000 - if [ "\$links_count" -gt "\$max_links" ]; then - echo "Link count exceeded \$max_links for ${bundle_file}." - echo "Try to shrink the number of links by increasing the max_gap and min_bundle_size options in the config file." - exit 1 - fi - - cat $karyotype > "karyotype.tsv" - cat $bundle_file | awk '{print \$1,\$2,\$3,\$4,\$5,\$6,\$7}' OFS="\\t" > bundled.links.tsv - - num_sequences=\$(cat $karyotype | wc -l) - if (( \$num_sequences <= 10 )); then - label_font_size=40 - elif (( \$num_sequences <= 30 )); then - label_font_size=30 - else - label_font_size=15 - fi - - if (( \$num_sequences <= 10 )); then - ticks_config=" - radius = dims(ideogram,radius_outer) - orientation = out - label_multiplier = 1e-6 - color = black - thickness = 5p - label_offset = 5p - - spacing = 0.5u - size = 10p - show_label = yes - label_size = 20p - format = %.1f - - - spacing = 1.0u - size = 15p - show_label = yes - label_size = 30p - format = %.1f - - " - - label_offset=" + 120p" - else - ticks_config="" - - label_offset=" + 25p" - fi - - cat <<-END_CONF > circos.conf - # circos.conf - karyotype = $karyotype - - - - default = 0.005r - - - radius = 0.8r - thickness = 25p - fill = yes - stroke_thickness = 0 - - show_label = yes - label_font = default - label_radius = dims(ideogram,radius_outer)\$label_offset - label_size = \$label_font_size - label_parallel = yes - - - - radius = 0.99r - crest = 1 - ribbon = yes - flat = yes - stroke_thickness = 0 - color = grey_a3 - - bezier_radius = 0r - bezier_radius_purity = 0.5 - - file = bundled.links.tsv - - - - show_ticks = yes - show_tick_labels = yes - chromosomes_units = 1000000 - chromosomes_display_default = yes - - \$ticks_config - - - <> - - <> - <> - END_CONF - - if [ ! -s $karyotype ]; then - touch "${target_on_ref_seq}.svg" - touch "${target_on_ref_seq}.png" - exit 0 - fi - - circos - - mv circos.svg "${target_on_ref_seq}.svg" - mv circos.png "${target_on_ref_seq}.png" - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - circos: \$(circos -version | awk '{print \$2}' FS='|' | tr -d '[:space:]') - perl: \$(circos -version | awk '{print \$4}' FS='|' | tr -d '[:space:]Perl') - END_VERSIONS - """ -} diff --git a/subworkflows/nf-core/fasta_explore_search_plot_tidk/main.nf b/subworkflows/nf-core/fasta_explore_search_plot_tidk/main.nf deleted file mode 100644 index 9dc3e6cc..00000000 --- a/subworkflows/nf-core/fasta_explore_search_plot_tidk/main.nf +++ /dev/null @@ -1,90 +0,0 @@ -include { SEQKIT_SEQ as FILTER_BY_LENGTH } from '../../../modules/nf-core/seqkit/seq/main' -include { SEQKIT_SORT as SORT_BY_LENGTH } from '../../../modules/nf-core/seqkit/sort/main' -include { TIDK_EXPLORE } from '../../../modules/nf-core/tidk/explore/main' -include { TIDK_SEARCH as TIDK_SEARCH_APRIORI } from '../../../modules/nf-core/tidk/search/main' -include { TIDK_SEARCH as TIDK_SEARCH_APOSTERIORI } from '../../../modules/nf-core/tidk/search/main' -include { TIDK_PLOT as TIDK_PLOT_APRIORI } from '../../../modules/nf-core/tidk/plot/main' -include { TIDK_PLOT as TIDK_PLOT_APOSTERIORI } from '../../../modules/nf-core/tidk/plot/main' - - -workflow FASTA_EXPLORE_SEARCH_PLOT_TIDK { - - take: - ch_fasta // channel: [ val(meta), [ fasta ] ] - ch_apriori_sequence // channel: [ val(meta), val(sequence) ]; Optional: Set to [] if not needed - // val(meta) from ch_fasta and ch_apriori_sequence are only required to have - // the same `id` - - main: - ch_versions = Channel.empty() - - // MODULE: SEQKIT_SEQ as FILTER_BY_LENGTH - FILTER_BY_LENGTH ( ch_fasta ) - - ch_filtered_fasta = FILTER_BY_LENGTH.out.fastx - ch_versions = ch_versions.mix(FILTER_BY_LENGTH.out.versions.first()) - - // MODULE: SEQKIT_SORT as SORT_BY_LENGTH - SORT_BY_LENGTH ( ch_filtered_fasta ) - - ch_sorted_fasta = SORT_BY_LENGTH.out.fastx - ch_versions = ch_versions.mix(SORT_BY_LENGTH.out.versions.first()) - - // TIDK_EXPLORE - TIDK_EXPLORE ( ch_filtered_fasta ) - - ch_top_sequence = TIDK_EXPLORE.out.top_sequence - ch_versions = ch_versions.mix(TIDK_EXPLORE.out.versions.first()) - - // TIDK_SEARCH as TIDK_SEARCH_APRIORI - ch_apriori_inputs = ch_sorted_fasta - | map { meta, fasta -> [ meta.id, meta, fasta ] } - | join( - ( ch_apriori_sequence ?: Channel.empty() ) - | map { meta, seq -> [ meta.id, seq ] } - ) - | map { id, meta, fasta, seq -> [ meta, fasta, seq ] } - - TIDK_SEARCH_APRIORI ( - ch_apriori_inputs.map { meta, fasta, seq -> [ meta, fasta ] }, - ch_apriori_inputs.map { meta, fasta, seq -> seq } - ) - - ch_apriori_tsv = TIDK_SEARCH_APRIORI.out.tsv - ch_versions = ch_versions.mix(TIDK_SEARCH_APRIORI.out.versions.first()) - - // TIDK_SEARCH as TIDK_SEARCH_APOSTERIORI - ch_aposteriori_inputs = ch_sorted_fasta - | join(ch_top_sequence) - | map { meta, fasta, txt -> - [ meta, fasta, txt.getText().strip() ] - } - - TIDK_SEARCH_APOSTERIORI ( - ch_aposteriori_inputs.map { meta, fasta, seq -> [ meta, fasta ] }, - ch_aposteriori_inputs.map { meta, fasta, seq -> seq } - ) - - ch_aposteriori_tsv = TIDK_SEARCH_APOSTERIORI.out.tsv - ch_versions = ch_versions.mix(TIDK_SEARCH_APOSTERIORI.out.versions.first()) - - // TIDK_PLOT as TIDK_PLOT_APRIORI - TIDK_PLOT_APRIORI ( ch_apriori_tsv ) - - ch_apriori_svg = TIDK_PLOT_APRIORI.out.svg - ch_versions = ch_versions.mix(TIDK_PLOT_APRIORI.out.versions.first()) - - // TIDK_PLOT as TIDK_PLOT_APOSTERIORI - TIDK_PLOT_APOSTERIORI ( ch_aposteriori_tsv ) - - ch_aposteriori_svg = TIDK_PLOT_APOSTERIORI.out.svg - ch_versions = ch_versions.mix(TIDK_PLOT_APOSTERIORI.out.versions.first()) - - emit: - apriori_tsv = ch_apriori_tsv // channel: [ val(meta), tsv ] - apriori_svg = ch_apriori_svg // channel: [ val(meta), svg ] - aposteriori_sequence = ch_top_sequence // channel: [ val(meta), txt ] - aposteriori_tsv = ch_aposteriori_tsv // channel: [ val(meta), tsv ] - aposteriori_svg = ch_aposteriori_svg // channel: [ val(meta), svg ] - versions = ch_versions // channel: [ versions.yml ] -} diff --git a/subworkflows/nf-core/fasta_explore_search_plot_tidk/meta.yml b/subworkflows/nf-core/fasta_explore_search_plot_tidk/meta.yml deleted file mode 100644 index 80bd15fd..00000000 --- a/subworkflows/nf-core/fasta_explore_search_plot_tidk/meta.yml +++ /dev/null @@ -1,74 +0,0 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json -name: "fasta_explore_search_plot_tidk" -description: | - Uses Telomere Identification toolKit (TIDK) to identify the frequency of telomeric repeats - along a sliding window for each sequence in the input fasta file. Results are presented in - TSV and SVG formats. The user can specify an a priori sequence for identification. - Possible a posteriori sequences are also explored and the most frequent sequence is - used for identification similar to the a priori sequence. seqkit/seq and seqkit/sort modules are - also included to filter out small sequences and sort sequences by length. -keywords: - - genomics - - telomere - - repeat - - search - - plot -components: - - seqkit/seq - - seqkit/sort - - tidk/explore - - tidk/plot - - tidk/search -input: - - ch_fasta: - type: file - description: | - Input assembly - Structure: [ val(meta), path(fasta) ] - pattern: "*.{fsa/fa/fasta}" - - ch_apriori_sequence: - type: string - description: | - A priori sequence - Structure: [ val(meta), val(sequence) ] -output: - - apriori_tsv: - type: file - description: | - Frequency table for the identification of the a priori sequence - Structure: [ val(meta), path(tsv) ] - pattern: "*.tsv" - - apriori_svg: - type: file - description: | - Frequency graph for the identification of the a priori sequence - Structure: [ val(meta), path(svg) ] - pattern: "*.svg" - - aposteriori_sequence: - type: file - description: | - The most frequent a posteriori sequence - Structure: [ val(meta), path(txt) ] - pattern: "*.txt" - - aposteriori_tsv: - type: file - description: | - Frequency table for the identification of the a aposteriori sequence - Structure: [ val(meta), path(tsv) ] - pattern: "*.tsv" - - aposteriori_svg: - type: file - description: | - Frequency graph for the identification of the a aposteriori sequence - Structure: [ val(meta), path(svg) ] - pattern: "*.svg" - - versions: - type: file - description: | - File containing software versions - Structure: [ path(versions.yml) ] - pattern: "versions.yml" -authors: - - "@GallVp" -maintainers: - - "@GallVp" diff --git a/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test b/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test deleted file mode 100644 index 9f69267d..00000000 --- a/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test +++ /dev/null @@ -1,119 +0,0 @@ -nextflow_workflow { - - name "Test Workflow FASTA_EXPLORE_SEARCH_PLOT_TIDK" - script "../main.nf" - workflow "FASTA_EXPLORE_SEARCH_PLOT_TIDK" - config './nextflow.config' - - tag "subworkflows" - tag "subworkflows_nfcore" - tag "subworkflows/fasta_explore_search_plot_tidk" - tag "fasta_explore_search_plot_tidk" - tag "seqkit/seq" - tag "seqkit/sort" - tag "tidk/explore" - tag "tidk/search" - tag "tidk/plot" - - test("homo_sapiens-genome_fasta-genome_21_fasta") { - - when { - workflow { - """ - input[0] = Channel.of( - [ - [ id:'test' ], - file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) - ], - [ - [ id:'test2' ], - file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) - ] - ) - input[1] = Channel.of( - [ - [ id:'test' ], - 'TTAGGG' - ], - [ - [ id:'test2' ], - 'TTAGGG' - ] - ) - """ - } - } - - then { - assertAll( - { assert workflow.success}, - { assert snapshot(workflow.out).match()} - ) - } - } - - test("homo_sapiens-genome_fasta-genome_21_fasta-partial_apriori-stub") { - - options '-stub' - - when { - workflow { - """ - input[0] = Channel.of( - [ - [ id:'test' ], - file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) - ], - [ - [ id:'test2' ], - file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) - ] - ) - input[1] = Channel.of( - [ - [ id:'test' ], - 'TTAGGG' - ] - ) - """ - } - } - - then { - assertAll( - { assert workflow.success}, - { assert snapshot(workflow.out).match()} - ) - } - } - - test("homo_sapiens-genome_fasta-genome_21_fasta-no_apriori-stub") { - - options '-stub' - - when { - workflow { - """ - input[0] = Channel.of( - [ - [ id:'test' ], - file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) - ], - [ - [ id:'test2' ], - file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) - ] - ) - input[1] = [] - """ - } - } - - then { - assertAll( - { assert workflow.success}, - { assert snapshot(workflow.out).match()} - ) - } - } -} \ No newline at end of file diff --git a/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test.snap b/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test.snap deleted file mode 100644 index 6e4e99c6..00000000 --- a/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test.snap +++ /dev/null @@ -1,389 +0,0 @@ -{ - "homo_sapiens-genome_fasta-genome_21_fasta": { - "content": [ - { - "0": [ - [ - { - "id": "test2" - }, - "test2.tsv:md5,4a42ed016c022a8238739e4acddf649c" - ], - [ - { - "id": "test" - }, - "test.tsv:md5,5b44a89396f412eb571ea240ef9deedd" - ] - ], - "1": [ - [ - { - "id": "test2" - }, - "test2.svg:md5,e76985fdc220867a05967984c2c4675d" - ], - [ - { - "id": "test" - }, - "test.svg:md5,d1bca1077cd52ad8c5126a4c9614d8f7" - ] - ], - "2": [ - [ - { - "id": "test2" - }, - "test2.top.sequence.txt:md5,bd63900958df06516e45b887072d788f" - ] - ], - "3": [ - [ - { - "id": "test2" - }, - "test2.tsv:md5,43f358de532d30e02e65339db07e4e00" - ] - ], - "4": [ - [ - { - "id": "test2" - }, - "test2.svg:md5,f0a6d4bb1464125bcbb6b54a471c1e3c" - ] - ], - "5": [ - "versions.yml:md5,02d48eb43c3882d9832c88b9e080b420", - "versions.yml:md5,506585e66b23f17620bf582ef60af56d", - "versions.yml:md5,5f9958ea613eceae4b09bb42ecaac656", - "versions.yml:md5,6713d71b01fe0402e5d32f1a5a181e0f", - "versions.yml:md5,83a6abbf9b68ec1e6152c5b6eb12f0d7", - "versions.yml:md5,b900c2a21c17ac2d16e33641e64fc7c1", - "versions.yml:md5,d0a1f1950f8dc5eb106b04a9364c8f04" - ], - "aposteriori_sequence": [ - [ - { - "id": "test2" - }, - "test2.top.sequence.txt:md5,bd63900958df06516e45b887072d788f" - ] - ], - "aposteriori_svg": [ - [ - { - "id": "test2" - }, - "test2.svg:md5,f0a6d4bb1464125bcbb6b54a471c1e3c" - ] - ], - "aposteriori_tsv": [ - [ - { - "id": "test2" - }, - "test2.tsv:md5,43f358de532d30e02e65339db07e4e00" - ] - ], - "apriori_svg": [ - [ - { - "id": "test2" - }, - "test2.svg:md5,e76985fdc220867a05967984c2c4675d" - ], - [ - { - "id": "test" - }, - "test.svg:md5,d1bca1077cd52ad8c5126a4c9614d8f7" - 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"versions.yml:md5,d0a1f1950f8dc5eb106b04a9364c8f04" - ] - } - ], - "timestamp": "2023-12-21T11:15:25.633714" - } -} \ No newline at end of file diff --git a/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/nextflow.config b/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/nextflow.config deleted file mode 100644 index 22d4fde0..00000000 --- a/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/nextflow.config +++ /dev/null @@ -1,19 +0,0 @@ -process { - - withName: FILTER_BY_LENGTH { - ext.prefix = { "${meta.id}.filtered" } - } - - withName: SORT_BY_LENGTH { - ext.args = '--quiet --reverse --by-length' - ext.prefix = { "${meta.id}.sorted" } - } - - withName: TIDK_EXPLORE { - ext.args = '--minimum 5 --maximum 30' - } - - withName: 'TIDK_SEARCH_*' { - ext.args = '--extension tsv' - } -} diff --git a/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/tags.yml b/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/tags.yml deleted file mode 100644 index d2d150d7..00000000 --- a/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -subworkflows/fasta_explore_search_plot_tidk: - - subworkflows/nf-core/fasta_explore_search_plot_tidk/** diff --git a/subworkflows/nf-core/fastq_trim_fastp_fastqc/main.nf b/subworkflows/nf-core/fastq_trim_fastp_fastqc/main.nf deleted file mode 100644 index 4f1c84fc..00000000 --- a/subworkflows/nf-core/fastq_trim_fastp_fastqc/main.nf +++ /dev/null @@ -1,103 +0,0 @@ -// -// Read QC and trimming -// - -include { FASTQC as FASTQC_RAW } from '../../../modules/nf-core/fastqc/main' -include { FASTQC as FASTQC_TRIM } from '../../../modules/nf-core/fastqc/main' -include { FASTP } from '../../../modules/nf-core/fastp/main' - -// -// Function that parses fastp json output file to get total number of reads after trimming -// -import groovy.json.JsonSlurper - -def getFastpReadsAfterFiltering(json_file) { - def Map json = (Map) new JsonSlurper().parseText(json_file.text).get('summary') - return json['after_filtering']['total_reads'].toLong() -} - -workflow FASTQ_TRIM_FASTP_FASTQC { - take: - ch_reads // channel: [ val(meta), path(reads) ] - ch_adapter_fasta // channel: [ path(fasta) ] - val_save_trimmed_fail // value: boolean - val_save_merged // value: boolean - val_skip_fastp // value: boolean - val_skip_fastqc // value: boolean - - main: - - ch_versions = Channel.empty() - - ch_fastqc_raw_html = Channel.empty() - ch_fastqc_raw_zip = Channel.empty() - if (!val_skip_fastqc) { - FASTQC_RAW ( - ch_reads - ) - ch_fastqc_raw_html = FASTQC_RAW.out.html - ch_fastqc_raw_zip = FASTQC_RAW.out.zip - ch_versions = ch_versions.mix(FASTQC_RAW.out.versions.first()) - } - - ch_trim_reads = ch_reads - ch_trim_json = Channel.empty() - ch_trim_html = Channel.empty() - ch_trim_log = Channel.empty() - ch_trim_reads_fail = Channel.empty() - ch_trim_reads_merged = Channel.empty() - ch_fastqc_trim_html = Channel.empty() - ch_fastqc_trim_zip = Channel.empty() - if (!val_skip_fastp) { - FASTP ( - ch_reads, - ch_adapter_fasta, - val_save_trimmed_fail, - val_save_merged - ) - ch_trim_reads = FASTP.out.reads - ch_trim_json = FASTP.out.json - ch_trim_html = FASTP.out.html - ch_trim_log = FASTP.out.log - ch_trim_reads_fail = FASTP.out.reads_fail - ch_trim_reads_merged = FASTP.out.reads_merged - ch_versions = ch_versions.mix(FASTP.out.versions.first()) - - // - // Filter empty FastQ files after adapter trimming so FastQC doesn't fail - // - ch_trim_reads - .join(ch_trim_json) - .map { - meta, reads, json -> - if (getFastpReadsAfterFiltering(json) > 0) { - [ meta, reads ] - } - } - .set { ch_trim_reads } - - if (!val_skip_fastqc) { - FASTQC_TRIM ( - ch_trim_reads - ) - ch_fastqc_trim_html = FASTQC_TRIM.out.html - ch_fastqc_trim_zip = FASTQC_TRIM.out.zip - ch_versions = ch_versions.mix(FASTQC_TRIM.out.versions.first()) - } - } - - emit: - reads = ch_trim_reads // channel: [ val(meta), path(reads) ] - trim_json = ch_trim_json // channel: [ val(meta), path(json) ] - trim_html = ch_trim_html // channel: [ val(meta), path(html) ] - trim_log = ch_trim_log // channel: [ val(meta), path(log) ] - trim_reads_fail = ch_trim_reads_fail // channel: [ val(meta), path(fastq.gz) ] - trim_reads_merged = ch_trim_reads_merged // channel: [ val(meta), path(fastq.gz) ] - - fastqc_raw_html = ch_fastqc_raw_html // channel: [ val(meta), path(html) ] - fastqc_raw_zip = ch_fastqc_raw_zip // channel: [ val(meta), path(zip) ] - fastqc_trim_html = ch_fastqc_trim_html // channel: [ val(meta), path(html) ] - fastqc_trim_zip = ch_fastqc_trim_zip // channel: [ val(meta), path(zip) ] - - versions = ch_versions.ifEmpty(null) // channel: [ path(versions.yml) ] -} diff --git a/subworkflows/nf-core/fastq_trim_fastp_fastqc/meta.yml b/subworkflows/nf-core/fastq_trim_fastp_fastqc/meta.yml deleted file mode 100644 index 9f4e12e0..00000000 --- a/subworkflows/nf-core/fastq_trim_fastp_fastqc/meta.yml +++ /dev/null @@ -1,108 +0,0 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json -name: "fastq_trim_fastp_fastqc" -description: Read QC, fastp trimming and read qc -keywords: - - qc - - quality_control - - adapters - - trimming - - fastq -components: - - fastqc - - fastp -input: - - ch_reads: - type: file - description: | - Structure: [ val(meta), path (reads) ] - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ], List of input FastQ files of size 1 and 2 for single-end and paired-end data, - respectively. If you wish to run interleaved paired-end data, supply as single-end data - but with `--interleaved_in` in your `modules.conf`'s `ext.args` for the module. - - ch_adapter_fasta: - type: file - description: | - Structure: path(adapter_fasta) - File in FASTA format containing possible adapters to remove. - - val_save_trimmed_fail: - type: boolean - description: | - Structure: val(save_trimmed_fail) - Specify true to save files that failed to pass trimming thresholds ending in `*.fail.fastq.gz` - - val_save_merged: - type: boolean - description: | - Structure: val(save_merged) - Specify true to save all merged reads to the a file ending in `*.merged.fastq.gz` - - val_skip_fastqc: - type: boolean - description: | - Structure: val(skip_fastqc) - skip the fastqc process if true - - val_skip_fastp: - type: boolean - description: | - Structure: val(skip_fastp) - skip the fastp process if true -output: - - meta: - type: value - description: Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: | - Structure: [ val(meta), path(reads) ] - The trimmed/modified/unmerged fastq reads - - trim_json: - type: file - description: | - Structure: [ val(meta), path(trim_json) ] - Results in JSON format - - trim_html: - type: file - description: | - Structure: [ val(meta), path(trim_html) ] - Results in HTML format - - trim_log: - type: file - description: | - Structure: [ val(meta), path(trim_log) ] - fastq log file - - trim_reads_fail: - type: file - description: | - Structure: [ val(meta), path(trim_reads_fail) ] - Reads the failed the preprocessing - - trim_reads_merged: - type: file - description: | - Structure: [ val(meta), path(trim_reads_merged) ] - Reads that were successfully merged - - fastqc_raw_html: - type: file - description: | - Structure: [ val(meta), path(fastqc_raw_html) ] - Raw fastQC report - - fastqc_raw_zip: - type: file - description: | - Structure: [ val(meta), path(fastqc_raw_zip) ] - Raw fastQC report archive - - fastqc_trim_html: - type: file - description: | - Structure: [ val(meta), path(fastqc_trim_html) ] - Trimmed fastQC report - - fastqc_trim_zip: - type: file - description: | - Structure: [ val(meta), path(fastqc_trim_zip) ] - Trimmed fastQC report archive - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@Joon-Klaps" -maintainers: - - "@Joon-Klaps" diff --git a/subworkflows/pfr/fasta_ltrretriever_lai/main.nf b/subworkflows/pfr/fasta_ltrretriever_lai/main.nf deleted file mode 100644 index 733b53ca..00000000 --- a/subworkflows/pfr/fasta_ltrretriever_lai/main.nf +++ /dev/null @@ -1,110 +0,0 @@ -include { CUSTOM_SHORTENFASTAIDS } from '../../../modules/pfr/custom/shortenfastaids/main.nf' -include { EDTA_LTRHARVEST } from '../../../modules/pfr/edta/ltrharvest/main.nf' -include { LTRFINDER } from '../../../modules/pfr/ltrfinder/main.nf' -include { LTRRETRIEVER } from '../../../modules/pfr/ltrretriever/main.nf' -include { CAT_CAT } from '../../../modules/pfr/cat/cat/main.nf' -include { LAI } from '../../../modules/pfr/lai/main.nf' -include { CUSTOM_RESTOREGFFIDS } from '../../../modules/pfr/custom/restoregffids/main.nf' - -workflow FASTA_LTRRETRIEVER_LAI { - - take: - ch_fasta // channel: [ val(meta), fasta ] - ch_monoploid_seqs // channel: [ val(meta), txt ]; Optional: Set to [] if not needed - skip_lai // val; true|false - - main: - - ch_versions = Channel.empty() - - // MOUDLE: CUSTOM_SHORTENFASTAIDS - CUSTOM_SHORTENFASTAIDS ( ch_fasta ) - - ch_short_ids_fasta = ch_fasta - | join(CUSTOM_SHORTENFASTAIDS.out.short_ids_fasta, by:0, remainder:true) - | map { meta, fasta, short_ids_fasta -> - [ meta, short_ids_fasta ?: fasta ] - } - - ch_short_ids_tsv = CUSTOM_SHORTENFASTAIDS.out.short_ids_tsv - ch_versions = ch_versions.mix(CUSTOM_SHORTENFASTAIDS.out.versions.first()) - - // MODULE: EDTA_LTRHARVEST - EDTA_LTRHARVEST ( ch_short_ids_fasta ) - - ch_ltrharvest_scn = EDTA_LTRHARVEST.out.scn - ch_versions = ch_versions.mix(EDTA_LTRHARVEST.out.versions.first()) - - // MODULE: LTRFINDER - LTRFINDER ( ch_short_ids_fasta ) - - ch_ltrfinder_scn = LTRFINDER.out.scn - ch_versions = ch_versions.mix(LTRFINDER.out.versions.first()) - - // MODULE: CAT_CAT - CAT_CAT ( ch_ltrharvest_scn.mix(ch_ltrfinder_scn).groupTuple() ) - - ch_ltr_candidates = CAT_CAT.out.file_out - ch_versions = ch_versions.mix(CAT_CAT.out.versions.first()) - - // MODULE: LTRRETRIEVER - ch_ltrretriever_inputs = ch_short_ids_fasta.join(ch_ltr_candidates) - LTRRETRIEVER ( - ch_ltrretriever_inputs.map { meta, fasta, ltr -> [ meta, fasta ] }, - ch_ltrretriever_inputs.map { meta, fasta, ltr -> ltr }, - [], - [], - [] - ) - - ch_pass_list = LTRRETRIEVER.out.pass_list - ch_ltrlib = LTRRETRIEVER.out.ltrlib - ch_annotation_out = LTRRETRIEVER.out.annotation_out - ch_annotation_gff = LTRRETRIEVER.out.annotation_gff - ch_versions = ch_versions.mix(LTRRETRIEVER.out.versions.first()) - - // MODULE: LAI - ch_lai_inputs = skip_lai - ? Channel.empty() - : ch_short_ids_fasta - | join(ch_pass_list) - | join(ch_annotation_out) - | join( - ch_monoploid_seqs ?: Channel.empty(), - by:0, - remainder: true - ) - | map { meta, fasta, pass, out, mono -> - [ meta, fasta, pass, out, mono ?: [] ] - } - LAI ( - ch_lai_inputs.map { meta, fasta, pass, out, mono -> [ meta, fasta ] }, - ch_lai_inputs.map { meta, fasta, pass, out, mono -> pass }, - ch_lai_inputs.map { meta, fasta, pass, out, mono -> out }, - ch_lai_inputs.map { meta, fasta, pass, out, mono -> mono } - ) - - ch_lai_log = LAI.out.log - ch_lai_out = LAI.out.lai_out - ch_versions = ch_versions.mix(LAI.out.versions.first()) - - // MODULE: CUSTOM_RESTOREGFFIDS - ch_restorable_gff_tsv = ch_annotation_gff.join(ch_short_ids_tsv) - - CUSTOM_RESTOREGFFIDS ( - ch_restorable_gff_tsv.map { meta, gff, tsv -> [ meta, gff ] }, - ch_restorable_gff_tsv.map { meta, gff, tsv -> tsv } - ) - - ch_restored_gff = ch_annotation_gff - | join(CUSTOM_RESTOREGFFIDS.out.restored_ids_gff3, by:0, remainder:true) - | map { meta, gff, restored_gff -> [ meta, restored_gff ?: gff ] } - ch_versions = ch_versions.mix(CUSTOM_RESTOREGFFIDS.out.versions.first()) - - emit: - ltrlib = ch_ltrlib // channel: [ val(meta), fasta ] - annotation_gff = ch_restored_gff // channel: [ val(meta), gff ] - lai_log = ch_lai_log // channel: [ val(meta), log ] - lai_out = ch_lai_out // channel: [ val(meta), out ] - versions = ch_versions // channel: [ versions.yml ] -} diff --git a/subworkflows/pfr/fasta_ltrretriever_lai/meta.yml b/subworkflows/pfr/fasta_ltrretriever_lai/meta.yml deleted file mode 100644 index 20be97a5..00000000 --- a/subworkflows/pfr/fasta_ltrretriever_lai/meta.yml +++ /dev/null @@ -1,78 +0,0 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json -name: "fasta_ltrretriever_lai" -description: | - Performs long terminal retrotransposons (LTR) annotation with gt/ltrharvest, ltrfinder - and ltrretriever along with LTR assembly index (LAI). -keywords: - - genomics - - genome - - annotation - - repeat - - transposons - - retrotransposons - - stats - - qc -components: - - custom/shortenfastaids - - edta/ltrharvest - - ltrfinder - - ltrretriever - - cat/cat - - lai - - custom/restoregffids -input: - - ch_fasta: - type: file - description: | - Channel for the assembly fasta file - Structure: [ val(meta), path(fasta) ] - pattern: "*.{fsa/fa/fasta}" - - ch_monoploid_seqs: - type: file - description: | - Channel for providing a list of monoploid sequences - for correct estimation of LAI for polyploid genomes. - This parameter is useful when all the haplotypes are - stored in a single fasta file. - Structure: [ val(meta), path(txt) ] - pattern: "*.txt" - - skip_lai: - type: boolean - description: | - Skip LAI estimation - Structure: [ val(boolean) ] -output: - - ltrlib: - type: file - description: | - LTR library constructed by ltrretriever - Structure: [ val(meta), path(fasta) ] - pattern: "*.LTRlib.fa" - - annotation_gff: - type: file - description: | - Annotation based on the LTR library - Structure: [ val(meta), path(gff3) ] - pattern: "*.gff3" - - lai_log: - type: file - description: | - Log from LAI - Structure: [ val(meta), path(log) ] - pattern: "*.LAI.log" - - lai_out: - type: file - description: | - LAI output - Structure: [ val(meta), path(out) ] - pattern: "*.LAI.out" - - versions: - type: file - description: | - File containing software versions - Structure: [ path(versions.yml) ] - pattern: "versions.yml" -authors: - - "@GallVp" -maintainers: - - "@GallVp" diff --git a/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test b/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test deleted file mode 100644 index 03e0af52..00000000 --- a/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test +++ /dev/null @@ -1,59 +0,0 @@ -nextflow_workflow { - - name "Test Workflow FASTA_LTRRETRIEVER_LAI" - script "../main.nf" - workflow "FASTA_LTRRETRIEVER_LAI" - config "./nextflow.config" - - tag "subworkflows" - tag "subworkflows_nfcore" - tag "subworkflows/fasta_ltrretriever_lai" - tag "fasta_ltrretriever_lai" - tag "modules/nf-core/gunzip" - tag "custom/shortenfastaids" - tag "edta/ltrharvest" - tag "ltrfinder" - tag "ltrretriever" - tag "cat/cat" - tag "lai" - tag "custom/restoregffids" - - test("homo_sapiens_genome_21_fasta") { - - setup { - run("GUNZIP") { - script "../../../../modules/nf-core/gunzip" - - process { - """ - input[0] = [ - [ id:'test' ], - file('/Users/hrauxr/Projects/nf-modules/tests/data/zenodo/actinidia_chinensis/chr1_7M.fasta.gz', checkIfExists: true) - ] - """ - } - } - } - - when { - workflow { - """ - input[0] = GUNZIP.out.gunzip - input[1] = [] - input[2] = false - """ - } - } - - then { - assertAll( - { assert workflow.success }, - { assert file(workflow.out.annotation_gff[0][1]).text.contains('Gypsy_LTR_retrotransposon') }, - { assert file(workflow.out.lai_log[0][1]).text.contains('Calculate LAI:') }, - { assert file(workflow.out.lai_log[0][1]).text.contains('Done!') }, - { assert Math.abs(Float.parseFloat(path(workflow.out.lai_out[0][1]).text.split("\n")[1].split("\t")[6]) - 31.29) <= 1.0 }, - { assert file(workflow.out.ltrlib[0][1]).text.contains('#LTR/Gypsy') } - ) - } - } -} \ No newline at end of file diff --git a/subworkflows/pfr/fasta_ltrretriever_lai/tests/nextflow.config b/subworkflows/pfr/fasta_ltrretriever_lai/tests/nextflow.config deleted file mode 100644 index 1c1e4001..00000000 --- a/subworkflows/pfr/fasta_ltrretriever_lai/tests/nextflow.config +++ /dev/null @@ -1,14 +0,0 @@ -process { - - withName: EDTA_LTRHARVEST { - ext.prefix = { "${meta.id}_edta_ltrharvest" } - } - - withName: LTRFINDER { - ext.args = '-harvest_out -size 1000000 -time 300' - } - - withName: CAT_CAT { - ext.prefix = { "${meta.id}_ltrharvest_ltrfinder.tabout" } - } -} diff --git a/subworkflows/pfr/fasta_ltrretriever_lai/tests/tags.yml b/subworkflows/pfr/fasta_ltrretriever_lai/tests/tags.yml deleted file mode 100644 index 9c15c67e..00000000 --- a/subworkflows/pfr/fasta_ltrretriever_lai/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -subworkflows/fasta_ltrretriever_lai: - - subworkflows/pfr/fasta_ltrretriever_lai/** diff --git a/subworkflows/pfr/fastq_bwa_mem_samblaster/main.nf b/subworkflows/pfr/fastq_bwa_mem_samblaster/main.nf deleted file mode 100644 index 50e824a9..00000000 --- a/subworkflows/pfr/fastq_bwa_mem_samblaster/main.nf +++ /dev/null @@ -1,61 +0,0 @@ -include { BWA_INDEX } from '../../../modules/pfr/bwa/index/main' -include { BWA_MEM } from '../../../modules/pfr/bwa/mem/main' -include { SAMBLASTER } from '../../../modules/pfr/samblaster/main' - -workflow FASTQ_BWA_MEM_SAMBLASTER { - - take: - ch_fastq // channel: [ val(meta), [ fq ] ] - ch_reference // channel: [ val(meta2), fasta, index ]; fast | index - - main: - ch_versions = Channel.empty() - - ch_has_index = ch_reference - | branch { meta2, fasta, index -> - yes: index - no: !index - } - - // MODULE: BWA_INDEX - BWA_INDEX ( ch_has_index.no.map { meta2, fasta, index -> [ meta2, fasta ] } ) - - ch_bwa_index = BWA_INDEX.out.index - | mix( - ch_has_index.yes - | map { meta2, fasta, index -> - [ meta2, index ] - } - ) - - ch_versions = ch_versions.mix(BWA_INDEX.out.versions.first()) - - // MODULE: BWA_MEM - ch_mem_inputs = ch_fastq - | combine( - ch_bwa_index - ) - | map { meta, fq, meta2, index -> - [ meta + [ ref_id: meta2.id ], fq, index ] - } - - def sort_bam = false - BWA_MEM( - ch_mem_inputs.map { meta, fq, index -> [ meta, fq ] }, - ch_mem_inputs.map { meta, fq, index -> [ [], index ] }, - sort_bam - ) - - ch_mem_bam = BWA_MEM.out.bam - ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) - - // MODULE: SAMBLASTER - SAMBLASTER ( ch_mem_bam ) - - ch_blasted_bam = SAMBLASTER.out.bam - ch_versions = ch_versions.mix(SAMBLASTER.out.versions.first()) - - emit: - bam = SAMBLASTER.out.bam // channel: [ val(meta), bam ] - versions = ch_versions // channel: [ versions.yml ] -} diff --git a/subworkflows/pfr/fastq_bwa_mem_samblaster/meta.yml b/subworkflows/pfr/fastq_bwa_mem_samblaster/meta.yml deleted file mode 100644 index 6eaf302b..00000000 --- a/subworkflows/pfr/fastq_bwa_mem_samblaster/meta.yml +++ /dev/null @@ -1,52 +0,0 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json -name: "fastq_bwa_mem_samblaster" -description: Index fasta if needed, map reads with BWA MEM and filter with samblaster -keywords: - - sort - - bam - - duplicate marking -components: - - bwa/index - - bwa/mem - - samblaster -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: | - List of input FastQ files of size 1 and 2 for single-end and paired-end data, - respectively. - - meta2: - type: map - description: | - Groovy Map containing reference information - e.g. [ id:'genome' ] - - fasta: - type: file - description: Input genome fasta file - - index: - type: file - description: BWA genome index files - pattern: "Directory containing BWA index *.{amb,ann,bwt,pac,sa}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false, ref_id:'genome' ] - - bam: - type: file - description: Tagged or filtered BAM file - pattern: "*.bam" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@GallVp" -maintainers: - - "@GallVp" diff --git a/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test b/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test deleted file mode 100644 index 9c3e2b01..00000000 --- a/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test +++ /dev/null @@ -1,44 +0,0 @@ -nextflow_workflow { - - name "Test Subworkflow FASTQ_BWA_MEM_SAMBLASTER" - script "../main.nf" - workflow "FASTQ_BWA_MEM_SAMBLASTER" - config './nextflow.config' - - tag "subworkflows" - tag "subworkflows_nfcore" - tag "subworkflows/fastq_bwa_mem_samblaster" - tag "samblaster" - tag "bwa/index" - tag "bwa/mem" - - - test("sarscov2-fq-gz") { - - when { - workflow { - """ - input[0] = Channel.of( - [ - [ id:'test' ], - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) - ] - ] - ) - input[1] = Channel.of( - [ [ id: 'genome' ], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), [] ] - ) - """ - } - } - - then { - assertAll( - { assert workflow.success}, - { assert snapshot(workflow.out).match()} - ) - } - } -} \ No newline at end of file diff --git a/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test.snap b/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test.snap deleted file mode 100644 index e5a9b18e..00000000 --- a/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test.snap +++ /dev/null @@ -1,41 +0,0 @@ -{ - "sarscov2-fq-gz": { - "content": [ - { - "0": [ - [ - { - "id": "test", - "ref_id": "genome" - }, - "test.on.genome.samblaster.bam:md5,496319fc81c383a9ae6ab52592876c9b" - ] - ], - "1": [ - "versions.yml:md5,2ed54ca5e54063cb579273d0792465a7", - "versions.yml:md5,49d22cda9beaf6ea1a1ad838ef4a4255", - "versions.yml:md5,6a2baa7f2d1d555fe604e451624f414b" - ], - "bam": [ - [ - { - "id": "test", - "ref_id": "genome" - }, - "test.on.genome.samblaster.bam:md5,496319fc81c383a9ae6ab52592876c9b" - ] - ], - "versions": [ - "versions.yml:md5,2ed54ca5e54063cb579273d0792465a7", - "versions.yml:md5,49d22cda9beaf6ea1a1ad838ef4a4255", - "versions.yml:md5,6a2baa7f2d1d555fe604e451624f414b" - ] - } - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-08T19:37:23.464854" - } -} \ No newline at end of file diff --git a/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/nextflow.config b/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/nextflow.config deleted file mode 100644 index 23df52b2..00000000 --- a/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/nextflow.config +++ /dev/null @@ -1,11 +0,0 @@ -process { - withName: BWA_MEM { - ext.prefix = { "${meta.id}.on.${meta.ref_id}.bwa.mem" } - ext.args = '-5SP' - } - - withName: SAMBLASTER { - ext.prefix = { "${meta.id}.on.${meta.ref_id}.samblaster" } - ext.args3 = '-h -F 2316' - } -} diff --git a/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/tags.yml b/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/tags.yml deleted file mode 100644 index 810ecdd8..00000000 --- a/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -subworkflows/fastq_bwa_mem_samblaster: - - subworkflows/pfr/fastq_bwa_mem_samblaster/** diff --git a/subworkflows/pfr/gff3_validate/main.nf b/subworkflows/pfr/gff3_validate/main.nf deleted file mode 100644 index 5437c5a6..00000000 --- a/subworkflows/pfr/gff3_validate/main.nf +++ /dev/null @@ -1,61 +0,0 @@ -include { GT_GFF3 } from '../../../modules/pfr/gt/gff3/main' -include { GT_GFF3VALIDATOR } from '../../../modules/pfr/gt/gff3validator/main' -include { CUSTOM_CHECKGFF3FASTACORRESPONDENCE } from '../../../modules/pfr/custom/checkgff3fastacorrespondence/main' - -workflow GFF3_VALIDATE { - - take: - ch_gff3 // channel: [ val(meta), gff3 ] - ch_fasta // channel: [ val(meta), fasta ] - - main: - - ch_versions = Channel.empty() - - // MODULE: GT_GFF3 - GT_GFF3 ( ch_gff3 ) - ch_versions = ch_versions.mix(GT_GFF3.out.versions.first()) - - // MODULE: GT_GFF3VALIDATOR - GT_GFF3VALIDATOR ( GT_GFF3.out.gt_gff3 ) - ch_versions = ch_versions.mix(GT_GFF3VALIDATOR.out.versions.first()) - - // MODULE: CUSTOM_CHECKGFF3FASTACORRESPONDENCE - GT_GFF3VALIDATOR.out.success_log - | join ( - GT_GFF3.out.gt_gff3 - ) - | map { meta, log, gff3 -> [ meta, gff3 ] } - | join ( - ch_fasta - ) - | set { ch_gff3_fasta } - - CUSTOM_CHECKGFF3FASTACORRESPONDENCE ( - ch_gff3_fasta.map { meta, gff3, fasta -> [ meta, gff3 ] }, - ch_gff3_fasta.map { meta, gff3, fasta -> fasta } - ) - - ch_versions = ch_versions.mix(CUSTOM_CHECKGFF3FASTACORRESPONDENCE.out.versions.first()) - - CUSTOM_CHECKGFF3FASTACORRESPONDENCE.out.success_log - | join ( - ch_gff3_fasta.map { meta, gff3, fasta -> [ meta, gff3 ] } - ) - | map { meta, log, gff3 -> [ meta, gff3 ] } - | set { ch_valid_gff3 } - - GT_GFF3.out.error_log - | mix ( - GT_GFF3VALIDATOR.out.error_log - ) - | mix ( - CUSTOM_CHECKGFF3FASTACORRESPONDENCE.out.error_log - ) - | set { ch_log_for_invalid_gff3 } - - emit: - valid_gff3 = ch_valid_gff3 // channel: [ val(meta), gff3 ] - log_for_invalid_gff3 = ch_log_for_invalid_gff3 // channel: [ val(meta), log ] - versions = ch_versions // channel: [ versions.yml ] -} diff --git a/subworkflows/pfr/gff3_validate/meta.yml b/subworkflows/pfr/gff3_validate/meta.yml deleted file mode 100644 index 5dea12a5..00000000 --- a/subworkflows/pfr/gff3_validate/meta.yml +++ /dev/null @@ -1,50 +0,0 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json -name: gff3_validate -description: | - Validates a gff3 file using GenomeTools gt-gff3, gt-gff3validator and - checks its correspondence with a fasta file -keywords: - - genome - - gff3 - - annotation - - validation -components: - - gt/gff3 - - gt/gff3validator - - custom/checkgff3fastacorrespondence -input: - - ch_gff3: - type: file - description: | - Input channel containing a gff3 file - Structure: [ val(meta), path(gff3) ] - pattern: "*.{gff,gff3}" - - ch_fasta: - type: file - description: | - Input channel containing a fasta file - Structure: [ val(meta), path(fasta) ] - pattern: "*.{fsa,fa,fasta}" -output: - - valid_gff3: - type: file - description: | - Valid gff3 file - Structure: [ val(meta), path(gff3) ] - pattern: "*.gff3" - - log_for_invalid_gff3: - type: file - description: | - Error log if the gff3 file is invalid - Structure: [ val(meta), path(gff3) ] - pattern: "*.gff3" - - versions: - type: file - description: | - File containing software versions - Structure: [ path(versions.yml) ] - pattern: "versions.yml" -authors: - - "@GallVp" -maintainers: - - "@GallVp" diff --git a/subworkflows/pfr/gff3_validate/tests/main.nf.test b/subworkflows/pfr/gff3_validate/tests/main.nf.test deleted file mode 100644 index fc1d7622..00000000 --- a/subworkflows/pfr/gff3_validate/tests/main.nf.test +++ /dev/null @@ -1,86 +0,0 @@ -nextflow_workflow { - - name "Test Workflow GFF3_VALIDATE" - script "../main.nf" - workflow "GFF3_VALIDATE" - config "./nextflow.config" - - tag "subworkflows" - tag "subworkflows_nfcore" - tag "subworkflows/gff3_validate" - tag "gff3_validate" - tag "gt" - tag "gt/gff3'" - tag "gt/gff3validator'" - tag "custom" - tag "custom/checkgff3fastacorrespondence'" - - test("sarscov2-genome_gff3-genome_fasta-all_pass") { - - when { - workflow { - """ - input[0] = Channel.of([ [ id:'test' ], // meta map - file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) - ]) - input[1] = Channel.of([ [ id:'test' ], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) - ]) - """ - } - } - - then { - assertAll( - { assert workflow.success}, - { assert snapshot(workflow.out).match()} - ) - } - } - - test("homo_sapiens-genome_bed-genome_fasta-gt_gff3_fail") { - - when { - workflow { - """ - input[0] = Channel.of([ [ id:'test' ], // meta map - file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true) - ]) - input[1] = Channel.of([ [ id:'test' ], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) - ]) - """ - } - } - - then { - assertAll( - { assert workflow.success}, - { assert snapshot(workflow.out).match()} - ) - } - } - - test("sarscov2-genome_gff3-homo_sapiens-genome_fasta-correspondence_fail") { - - when { - workflow { - """ - input[0] = Channel.of([ [ id:'test' ], // meta map - file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) - ]) - input[1] = Channel.of([ [ id:'test' ], - file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) - ]) - """ - } - } - - then { - assertAll( - { assert workflow.success}, - { assert snapshot(workflow.out).match()} - ) - } - } -} \ No newline at end of file diff --git a/subworkflows/pfr/gff3_validate/tests/main.nf.test.snap b/subworkflows/pfr/gff3_validate/tests/main.nf.test.snap deleted file mode 100644 index 4d2a59b2..00000000 --- a/subworkflows/pfr/gff3_validate/tests/main.nf.test.snap +++ /dev/null @@ -1,115 +0,0 @@ -{ - "sarscov2-genome_gff3-genome_fasta-all_pass": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.gt.gff3:md5,2ae900237ace415557b8735fac088b85" - ] - ], - "1": [ - - ], - "2": [ - "versions.yml:md5,10fe5c201e5fcddb52c3607ab3fdfb34", - "versions.yml:md5,856745cef2fff087e50ea4c0ffa3addd", - "versions.yml:md5,a89255422a163684b0c80ebdd8ad28ae" - ], - "log_for_invalid_gff3": [ - - ], - "valid_gff3": [ - [ - { - "id": "test" - }, - "test.gt.gff3:md5,2ae900237ace415557b8735fac088b85" - ] - ], - "versions": [ - "versions.yml:md5,10fe5c201e5fcddb52c3607ab3fdfb34", - "versions.yml:md5,856745cef2fff087e50ea4c0ffa3addd", - "versions.yml:md5,a89255422a163684b0c80ebdd8ad28ae" - ] - } - ], - "timestamp": "2023-12-07T10:33:21.09887" - }, - "homo_sapiens-genome_bed-genome_fasta-gt_gff3_fail": { - "content": [ - { - "0": [ - - ], - "1": [ - [ - { - "id": "test" - }, - "test.error.log:md5,c096494c3cd02864eb54434c294ba382" - ] - ], - "2": [ - "versions.yml:md5,a89255422a163684b0c80ebdd8ad28ae" - ], - "log_for_invalid_gff3": [ - [ - { - "id": "test" - }, - "test.error.log:md5,c096494c3cd02864eb54434c294ba382" - ] - ], - "valid_gff3": [ - - ], - "versions": [ - "versions.yml:md5,a89255422a163684b0c80ebdd8ad28ae" - ] - } - ], - "timestamp": "2023-12-07T10:35:26.549003" - }, - "sarscov2-genome_gff3-homo_sapiens-genome_fasta-correspondence_fail": { - "content": [ - { - "0": [ - - ], - "1": [ - [ - { - "id": "test" - }, - "test.error.log:md5,67686ea1ef271821f1218a8fe0207e1f" - ] - ], - "2": [ - "versions.yml:md5,10fe5c201e5fcddb52c3607ab3fdfb34", - "versions.yml:md5,856745cef2fff087e50ea4c0ffa3addd", - "versions.yml:md5,a89255422a163684b0c80ebdd8ad28ae" - ], - "log_for_invalid_gff3": [ - [ - { - "id": "test" - }, - "test.error.log:md5,67686ea1ef271821f1218a8fe0207e1f" - ] - ], - "valid_gff3": [ - - ], - "versions": [ - "versions.yml:md5,10fe5c201e5fcddb52c3607ab3fdfb34", - "versions.yml:md5,856745cef2fff087e50ea4c0ffa3addd", - "versions.yml:md5,a89255422a163684b0c80ebdd8ad28ae" - ] - } - ], - "timestamp": "2023-12-07T10:35:32.53584" - } -} \ No newline at end of file diff --git a/subworkflows/pfr/gff3_validate/tests/nextflow.config b/subworkflows/pfr/gff3_validate/tests/nextflow.config deleted file mode 100644 index d07a8881..00000000 --- a/subworkflows/pfr/gff3_validate/tests/nextflow.config +++ /dev/null @@ -1,6 +0,0 @@ -process { - - withName: GT_GFF3 { - ext.args = '-tidy -retainids -addintrons' - } -} diff --git a/subworkflows/pfr/gff3_validate/tests/tags.yml b/subworkflows/pfr/gff3_validate/tests/tags.yml deleted file mode 100644 index 60ffbf0c..00000000 --- a/subworkflows/pfr/gff3_validate/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -subworkflows/gff3_validate: - - subworkflows/pfr/gff3_validate/** diff --git a/tower.yml b/tower.yml new file mode 100644 index 00000000..787aedfe --- /dev/null +++ b/tower.yml @@ -0,0 +1,5 @@ +reports: + multiqc_report.html: + display: "MultiQC HTML report" + samplesheet.csv: + display: "Auto-created samplesheet with collated metadata and FASTQ paths" diff --git a/version_check.sh b/version_check.sh deleted file mode 100755 index ce55eaba..00000000 --- a/version_check.sh +++ /dev/null @@ -1,23 +0,0 @@ -#!/usr/bin/env bash - -config_version=$(sed -n '/version/s/version//p' nextflow.config | tr -d " ='[:space:]}") -cff_version=$(sed -n '/^version: /s/version: //p' CITATION.cff | tr -d '[:space:]') - -if [[ $config_version != $cff_version ]]; then - echo 'config_version != cff_version' - exit 1 -fi - -# Check README version - -readme_citation_text=$(grep -A10 '## Citations' README.md) - -if [[ ! $readme_citation_text =~ "($config_version)" ]]; then - echo 'Failed to match README version' - exit 1 -fi - -# Check CHANGELOG version - -grep "Version $config_version" CHANGELOG.md >/dev/null \ - || (echo 'Failed to match CHANGELOG version'; exit 1) diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index b655c82f..db0a1dd5 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -1,375 +1,134 @@ -nextflow.enable.dsl=2 - -include { validateParams } from '../modules/local/utils' -include { jsonifyParams } from '../modules/local/utils' - -include { GUNZIP as GUNZIP_FASTA } from '../modules/nf-core/gunzip/main' -include { FASTAVALIDATOR } from '../modules/nf-core/fastavalidator/main' -include { GUNZIP as GUNZIP_GFF3 } from '../modules/nf-core/gunzip/main' -include { GFF3_VALIDATE } from '../subworkflows/pfr/gff3_validate/main' -include { GT_STAT } from '../modules/pfr/gt/stat/main' -include { NCBI_FCS_ADAPTOR } from '../modules/local/ncbi_fcs_adaptor' -include { NCBI_FCS_GX } from '../subworkflows/local/ncbi_fcs_gx' -include { ASSEMBLATHON_STATS } from '../modules/local/assemblathon_stats' -include { BUSCO } from '../subworkflows/local/busco' -include { FASTA_EXPLORE_SEARCH_PLOT_TIDK } from '../subworkflows/nf-core/fasta_explore_search_plot_tidk/main' -include { FASTA_LTRRETRIEVER_LAI } from '../subworkflows/pfr/fasta_ltrretriever_lai/main' -include { KRAKEN2 } from '../subworkflows/local/kraken2' -include { FASTQ_TRIM_FASTP_FASTQC } from '../subworkflows/nf-core/fastq_trim_fastp_fastqc/main' -include { HIC_CONTACT_MAP } from '../subworkflows/local/hic_contact_map' -include { SYNTENY } from '../subworkflows/local/synteny' -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' -include { CREATE_REPORT } from '../modules/local/create_report' - -validateParams(params) +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + PRINT PARAMS SUMMARY +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' + +def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) +def citation = '\n' + WorkflowMain.citation(workflow) + '\n' +def summary_params = paramsSummaryMap(workflow) + +// Print parameter summary log to screen +log.info logo + paramsSummaryLog(workflow) + citation + +WorkflowAssemblyqc.initialise(params, log) + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + CONFIG FILES +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) +ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath( params.multiqc_config, checkIfExists: true ) : Channel.empty() +ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath( params.multiqc_logo, checkIfExists: true ) : Channel.empty() +ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + IMPORT LOCAL MODULES/SUBWORKFLOWS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +// +// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules +// +include { INPUT_CHECK } from '../subworkflows/local/input_check' + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + IMPORT NF-CORE MODULES/SUBWORKFLOWS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +// +// MODULE: Installed directly from nf-core/modules +// +include { FASTQC } from '../modules/nf-core/fastqc/main' +include { MULTIQC } from '../modules/nf-core/multiqc/main' +include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + RUN MAIN WORKFLOW +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +// Info required for completion email and summary +def multiqc_report = [] workflow ASSEMBLYQC { - // Input channels - ch_versions = Channel.empty() - - ch_target_assemby_branch = Channel.fromList(params.target_assemblies) - | map { tag, fasta -> - [ [ id: tag ], file(fasta, checkIfExists: true) ] - } - | branch { meta, fasta -> - gz: "$fasta".endsWith(".gz") - rest: !"$fasta".endsWith(".gz") - } - - ch_assemby_gff3_branch = Channel.fromList(params.assembly_gff3) - | map { tag, gff3 -> - [ [ id: tag ], file(gff3, checkIfExists: true) ] - } - | branch { meta, gff3 -> - gz: "$gff3".endsWith(".gz") - rest: !"$gff3".endsWith(".gz") - } - - // MODULE: GUNZIP as GUNZIP_FASTA - GUNZIP_FASTA ( ch_target_assemby_branch.gz ) - - ch_target_assembly = GUNZIP_FASTA.out.gunzip.mix(ch_target_assemby_branch.rest) - ch_versions = ch_versions.mix(GUNZIP_FASTA.out.versions.first()) - - - // MODULE: GUNZIP as GUNZIP_GFF3 - GUNZIP_GFF3 ( ch_assemby_gff3_branch.gz ) + ch_versions = Channel.empty() - ch_assembly_gff3 = GUNZIP_GFF3.out.gunzip.mix(ch_assemby_gff3_branch.rest) - ch_versions = ch_versions.mix(GUNZIP_GFF3.out.versions.first()) - - // MODULE: FASTAVALIDATOR - FASTAVALIDATOR ( ch_target_assembly ) - - ch_valid_target_assembly = ch_target_assembly.join(FASTAVALIDATOR.out.success_log) - | map { meta, fasta, log -> [ meta, fasta ] } - - ch_invalid_assembly_log = FASTAVALIDATOR.out.error_log - | map { meta, error_log -> - log.warn("FASTA validation failed for ${meta.id}\n${error_log.text}") - - [ meta, error_log ] - } - - ch_versions = ch_versions.mix(FASTAVALIDATOR.out.versions.first()) - - // SUBWORKFLOW: GFF3_VALIDATE - GFF3_VALIDATE ( - ch_assembly_gff3, - ch_valid_target_assembly + // + // SUBWORKFLOW: Read in samplesheet, validate and stage input files + // + INPUT_CHECK ( + file(params.input) ) - - ch_valid_gff3 = GFF3_VALIDATE.out.valid_gff3 - - ch_invalid_gff3_log = GFF3_VALIDATE.out.log_for_invalid_gff3 - | map { meta, error_log -> - log.warn("GFF3 validation failed for ${meta.id}\n${error_log.text}") - - [ meta, error_log ] - } - - ch_versions = ch_versions.mix(GFF3_VALIDATE.out.versions) - - // MODULE: GT_STAT - GT_STAT ( ch_valid_gff3 ) - - ch_gt_stats = GT_STAT.out.stats - | map { meta, yml -> yml } - - ch_versions = ch_versions.mix(GT_STAT.out.versions.first()) - - // MODULE: NCBI_FCS_ADAPTOR - ch_fcs_adaptor_inputs = params.ncbi_fcs_adaptor.skip - ? Channel.empty() - : ch_valid_target_assembly - | map { meta, fa -> [ meta.id, fa ] } - - NCBI_FCS_ADAPTOR ( ch_fcs_adaptor_inputs ) - - ch_fcs_adaptor_report = NCBI_FCS_ADAPTOR.out.report - | map { tag, report -> - def is_clean = file(report).readLines().size < 2 - - if (!is_clean) { - log.warn(""" - Adaptor contamination detected in ${tag}. - See the report for further details. - """.stripIndent()) - } - - [ tag, report ] - } - - ch_fcs_adaptor_passed_assembly = params.ncbi_fcs_adaptor.skip - ? ( - ch_valid_target_assembly - | map { meta, fa -> [ meta.id, fa ] } - ) - : ( - ch_fcs_adaptor_report - | map { tag, report -> - [ tag, file(report).readLines().size < 2 ] - } - | filter { tag, is_clean -> is_clean } - | join( - ch_valid_target_assembly - | map { meta, fa -> [ meta.id, fa ] } - ) - | map { tag, clean, fa -> - [ tag, fa ] - } - ) - - ch_versions = ch_versions.mix(NCBI_FCS_ADAPTOR.out.versions.first()) - - // SUBWORKFLOW: NCBI_FCS_GX - ch_fcs_gx_inputs = params.ncbi_fcs_gx.skip - ? Channel.empty() - : ch_valid_target_assembly - | map { meta, fa -> [ meta.id, fa ] } - | combine( Channel.of(file(params.ncbi_fcs_gx.db_path, checkIfExists:true)) ) - - NCBI_FCS_GX( - ch_fcs_gx_inputs.map { tag, fa, db -> [ tag, fa ] }, - ch_fcs_gx_inputs.map { tag, fa, db -> db } + ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) + // TODO: OPTIONAL, you can use nf-validation plugin to create an input channel from the samplesheet with Channel.fromSamplesheet("input") + // See the documentation https://nextflow-io.github.io/nf-validation/samplesheets/fromSamplesheet/ + // ! There is currently no tooling to help you write a sample sheet schema + + // + // MODULE: Run FastQC + // + FASTQC ( + INPUT_CHECK.out.reads ) + ch_versions = ch_versions.mix(FASTQC.out.versions.first()) - ch_fcs_gx_report = NCBI_FCS_GX.out.gx_report - | map { tag, report -> - def is_clean = file(report).readLines().size < 3 - - if (!is_clean) { - log.warn(""" - Foreign organism contamination detected in ${tag}. - See the report for further details. - """.stripIndent()) - } - - [ tag, report ] - } - - ch_fcs_gx_taxonomy_plot = NCBI_FCS_GX.out.gx_taxonomy_plot - | map { tag, cut, html -> [ tag, html ] } - - ch_fcs_gx_passed_assembly = params.ncbi_fcs_gx.skip - ? ( - ch_valid_target_assembly - | map { meta, fa -> [ meta.id, fa ] } - ) - : ( - ch_fcs_gx_report - | map { tag, report -> - [ tag, file(report).readLines().size < 3 ] - } - | filter { tag, is_clean -> is_clean } - | join( - ch_valid_target_assembly - | map { meta, fa -> [ meta.id, fa ] } - ) - | map { tag, clean, fa -> - [ tag, fa ] - } - ) - - ch_versions = ch_versions.mix(NCBI_FCS_GX.out.versions) - - ch_clean_assembly = ch_fcs_adaptor_passed_assembly - | join( - ch_fcs_gx_passed_assembly - ) - | map { tag, fa, fa2 -> - [ tag, fa ] - } - - // MODULE: ASSEMBLATHON_STATS - ASSEMBLATHON_STATS ( ch_clean_assembly ) - - ch_assemblathon_stats = ASSEMBLATHON_STATS.out.stats - ch_versions = ch_versions.mix(ASSEMBLATHON_STATS.out.versions.first()) - - - // SUBWORKFLOW: BUSCO - ch_busco_inputs = params.busco.skip - ? Channel.empty() - : ch_clean_assembly - | combine(Channel.fromList(params.busco.lineage_datasets)) - | map { tag, fa, lineage -> - [ tag, file(fa, checkIfExists: true), lineage ] - } - BUSCO ( ch_busco_inputs ) - - ch_busco_summary = BUSCO.out.summary - ch_busco_plot = BUSCO.out.plot - ch_versions = ch_versions.mix(BUSCO.out.versions) - - // SUBWORKFLOW: FASTA_EXPLORE_SEARCH_PLOT_TIDK - ch_tidk_inputs = params.tidk.skip - ? Channel.empty() - : ch_clean_assembly - | map { tag, fa -> [ [ id: tag ], fa ] } - | combine( - Channel.of(params.tidk.repeat_seq) - ) - - FASTA_EXPLORE_SEARCH_PLOT_TIDK( - ch_tidk_inputs.map { meta, fa, seq -> [ meta, fa ] }, - ch_tidk_inputs.map { meta, fa, seq -> [ meta, seq ] } - ) - - ch_tidk_outputs = FASTA_EXPLORE_SEARCH_PLOT_TIDK.out.apriori_svg - | mix(FASTA_EXPLORE_SEARCH_PLOT_TIDK.out.aposteriori_svg) - | mix(FASTA_EXPLORE_SEARCH_PLOT_TIDK.out.aposteriori_sequence) - | map { meta, file -> file } - | mix( - Channel.of("$params.tidk.repeat_seq") - | collectFile(name: 'a_priori.sequence', newLine: true) - ) - - ch_versions = ch_versions.mix(FASTA_EXPLORE_SEARCH_PLOT_TIDK.out.versions) - - // SUBWORKFLOW: FASTA_LTRRETRIEVER_LAI - ch_lai_inputs = params.lai.skip - ? Channel.empty() - : ch_clean_assembly - | join( - Channel.fromList(params.lai.monoploid_seqs) - | map { tag, seqs -> - [ tag, file(seqs, checkIfExists: true)] - }, remainder: true - ) - | map { id, fasta, mono -> [ id, fasta, mono ?: [] ] } - - FASTA_LTRRETRIEVER_LAI( - ch_lai_inputs.map { id, fasta, mono -> [ [ id:id ], fasta ] }, - ch_lai_inputs.map { id, fasta, mono -> [ [ id:id ], mono ] }, - false // Not skipping LAI using this flag + CUSTOM_DUMPSOFTWAREVERSIONS ( + ch_versions.unique().collectFile(name: 'collated_versions.yml') ) - ch_lai_outputs = FASTA_LTRRETRIEVER_LAI.out.lai_log - | join(FASTA_LTRRETRIEVER_LAI.out.lai_out, remainder: true) - | map { meta, log, out -> out ? [ log, out ] : [log] } - - ch_versions = ch_versions.mix(FASTA_LTRRETRIEVER_LAI.out.versions) - - // SUBWORKFLOW: KRAKEN2 - ch_kraken2_input_assembly = params.kraken2.skip - ? Channel.empty() - : ch_clean_assembly - - ch_kraken2_db_path = params.kraken2.skip - ? Channel.empty() - : Channel.of(file(params.kraken2.db_path, checkIfExists:true)) - KRAKEN2( - ch_kraken2_input_assembly, - ch_kraken2_db_path - ) - - ch_kraken2_plot = KRAKEN2.out.plot - ch_versions = ch_versions.mix(KRAKEN2.out.versions) - - // SUBWORKFLOW: FASTQ_TRIM_FASTP_FASTQC - ch_paired_reads = params.hic.skip - ? Channel.empty() - : ( - "${params.hic.paired_reads}".find(/.*[\/].*\.(fastq|fq)\.gz/) - ? Channel.fromFilePairs(params.hic.paired_reads, checkIfExists: true) - : Channel.fromSRA(params.hic.paired_reads) - ) - - FASTQ_TRIM_FASTP_FASTQC( - ch_paired_reads.map { sample, fq -> [ [ id: sample, single_end: false], fq ] }, - [], - true, // val_save_trimmed_fail - false, // val_save_merged - params.hic.skip_fastp, - params.hic.skip_fastqc - ) - - ch_cleaned_paired_reads = FASTQ_TRIM_FASTP_FASTQC.out.reads - ch_versions = ch_versions.mix(FASTQ_TRIM_FASTP_FASTQC.out.versions) - - // SUBWORKFLOW: HIC_CONTACT_MAP - ch_hic_input_assembly = params.hic.skip - ? Channel.empty() - : ch_clean_assembly - - HIC_CONTACT_MAP( - ch_cleaned_paired_reads, - ch_hic_input_assembly - ) - - ch_hic_map_html = HIC_CONTACT_MAP.out.html - ch_versions = ch_versions.mix(HIC_CONTACT_MAP.out.versions) - - // SUBWORKFLOW: SYNTENY - ch_clean_assembly_seq_list = params.synteny.skip - ? Channel.empty() - : ch_clean_assembly - | join( - Channel.fromList(params.synteny.assembly_seq_list) - | map { tag, seq_list -> - [ tag, file(seq_list, checkIfExists: true) ] - } - ) - - ch_xref_assembly = params.synteny.skip - ? Channel.empty() - : Channel.fromList(params.synteny.xref_assemblies) - | map { tag, xref_fa, xref_seq_list -> - [ tag, file(xref_fa, checkIfExists: true), file(xref_seq_list, checkIfExists: true) ] - } - - SYNTENY( - ch_clean_assembly_seq_list, - ch_xref_assembly - ) - - ch_synteny_plot = SYNTENY.out.plot - ch_versions = ch_versions.mix(SYNTENY.out.versions) - - // MODULE: CUSTOM_DUMPSOFTWAREVERSIONS - CUSTOM_DUMPSOFTWAREVERSIONS( - ch_versions - | unique() - | collectFile(name: 'collated_versions.yml') + // + // MODULE: MultiQC + // + workflow_summary = WorkflowAssemblyqc.paramsSummaryMultiqc(workflow, summary_params) + ch_workflow_summary = Channel.value(workflow_summary) + + methods_description = WorkflowAssemblyqc.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) + ch_methods_description = Channel.value(methods_description) + + ch_multiqc_files = Channel.empty() + ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) + ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + + MULTIQC ( + ch_multiqc_files.collect(), + ch_multiqc_config.toList(), + ch_multiqc_custom_config.toList(), + ch_multiqc_logo.toList() ) + multiqc_report = MULTIQC.out.report.toList() +} - // MODULE: CREATE REPORT - CREATE_REPORT( - ch_invalid_assembly_log .map { meta, file -> file }.collect().ifEmpty([]), - ch_invalid_gff3_log .map { meta, file -> file }.collect().ifEmpty([]), - ch_fcs_adaptor_report .map { meta, file -> file }.collect().ifEmpty([]), - ch_fcs_gx_report .mix(ch_fcs_gx_taxonomy_plot).map { meta, file -> file }.collect().ifEmpty([]), - ch_assemblathon_stats .collect().ifEmpty([]), - ch_gt_stats .collect().ifEmpty([]), - ch_busco_summary .mix(ch_busco_plot).collect().ifEmpty([]), - ch_tidk_outputs .collect().ifEmpty([]), - ch_lai_outputs .collect().ifEmpty([]), - ch_kraken2_plot .collect().ifEmpty([]), - ch_hic_map_html .collect().ifEmpty([]), - ch_synteny_plot .collect().ifEmpty([]), - CUSTOM_DUMPSOFTWAREVERSIONS .out.yml, - Channel.of ( jsonifyParams ( params ) ), - ) +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + COMPLETION EMAIL AND SUMMARY +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +workflow.onComplete { + if (params.email || params.email_on_fail) { + NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) + } + NfcoreTemplate.dump_parameters(workflow, params) + NfcoreTemplate.summary(workflow, params, log) + if (params.hook_url) { + NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log) + } } + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + THE END +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ From 8198830da1249750cf9e2d0208cad8937d5eb338 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 20 Feb 2024 14:00:07 +1300 Subject: [PATCH 08/65] A basic workflow now running successfully --- .github/ISSUE_TEMPLATE/bug_report.yml | 100 ++-- .github/workflows/ci.yml | 2 +- .github/workflows/download_pipeline.yml | 2 +- .nf-core.yml | 30 +- .pre-commit-config.yaml | 2 +- README.md | 2 +- assets/assemblysheet.csv | 2 + assets/samplesheet.csv | 3 - assets/schema_input.json | 59 ++- bin/check_samplesheet.py | 259 ----------- cleanNXF.sh | 11 + conf/igenomes.config | 440 ------------------ conf/modules.config | 27 -- conf/test.config | 8 +- conf/test_full.config | 8 +- lib/WorkflowAssemblyqc.groovy | 110 +---- lib/WorkflowMain.groovy | 10 +- main.nf | 17 +- modules.json | 10 +- modules/local/samplesheet_check.nf | 31 -- .../dumpsoftwareversions/environment.yml | 2 +- .../custom/dumpsoftwareversions/main.nf | 4 +- .../templates/dumpsoftwareversions.py | 3 +- .../dumpsoftwareversions/tests/main.nf.test | 7 +- .../tests/main.nf.test.snap | 50 +- .../nf-core/fastavalidator/environment.yml | 9 + modules/nf-core/fastavalidator/main.nf | 62 +++ modules/nf-core/fastavalidator/meta.yml | 53 +++ .../nf-core/fastavalidator/tests/main.nf.test | 60 +++ .../fastavalidator/tests/main.nf.test.snap | 76 +++ modules/nf-core/fastavalidator/tests/tags.yml | 2 + modules/nf-core/fastqc/main.nf | 55 --- modules/nf-core/fastqc/meta.yml | 57 --- modules/nf-core/fastqc/tests/main.nf.test | 109 ----- .../nf-core/fastqc/tests/main.nf.test.snap | 10 - modules/nf-core/fastqc/tests/tags.yml | 2 - .../{fastqc => gunzip}/environment.yml | 4 +- modules/nf-core/gunzip/main.nf | 48 ++ modules/nf-core/gunzip/meta.yml | 39 ++ modules/nf-core/gunzip/tests/main.nf.test | 36 ++ .../nf-core/gunzip/tests/main.nf.test.snap | 31 ++ modules/nf-core/gunzip/tests/tags.yml | 2 + modules/nf-core/multiqc/environment.yml | 7 - modules/nf-core/multiqc/main.nf | 55 --- modules/nf-core/multiqc/meta.yml | 59 --- modules/nf-core/multiqc/tests/main.nf.test | 63 --- modules/nf-core/multiqc/tests/tags.yml | 2 - nextflow.config | 119 +++-- nextflow_schema.json | 365 +++++++++++---- subworkflows/local/input_check.nf | 44 -- workflows/assemblyqc.nf | 115 ++--- 51 files changed, 1007 insertions(+), 1676 deletions(-) create mode 100644 assets/assemblysheet.csv delete mode 100644 assets/samplesheet.csv delete mode 100755 bin/check_samplesheet.py create mode 100755 cleanNXF.sh delete mode 100644 conf/igenomes.config mode change 100644 => 100755 main.nf delete mode 100644 modules/local/samplesheet_check.nf create mode 100644 modules/nf-core/fastavalidator/environment.yml create mode 100644 modules/nf-core/fastavalidator/main.nf create mode 100644 modules/nf-core/fastavalidator/meta.yml create mode 100644 modules/nf-core/fastavalidator/tests/main.nf.test create mode 100644 modules/nf-core/fastavalidator/tests/main.nf.test.snap create mode 100644 modules/nf-core/fastavalidator/tests/tags.yml delete mode 100644 modules/nf-core/fastqc/main.nf delete mode 100644 modules/nf-core/fastqc/meta.yml delete mode 100644 modules/nf-core/fastqc/tests/main.nf.test delete mode 100644 modules/nf-core/fastqc/tests/main.nf.test.snap delete mode 100644 modules/nf-core/fastqc/tests/tags.yml rename modules/nf-core/{fastqc => gunzip}/environment.yml (61%) create mode 100644 modules/nf-core/gunzip/main.nf create mode 100644 modules/nf-core/gunzip/meta.yml create mode 100644 modules/nf-core/gunzip/tests/main.nf.test create mode 100644 modules/nf-core/gunzip/tests/main.nf.test.snap create mode 100644 modules/nf-core/gunzip/tests/tags.yml delete mode 100644 modules/nf-core/multiqc/environment.yml delete mode 100644 modules/nf-core/multiqc/main.nf delete mode 100644 modules/nf-core/multiqc/meta.yml delete mode 100644 modules/nf-core/multiqc/tests/main.nf.test delete mode 100644 modules/nf-core/multiqc/tests/tags.yml delete mode 100644 subworkflows/local/input_check.nf diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml index c22d7e64..af436aa6 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.yml +++ b/.github/ISSUE_TEMPLATE/bug_report.yml @@ -2,53 +2,53 @@ name: Bug report description: Report something that is broken or incorrect labels: bug body: -- type: textarea - id: description - attributes: - label: Description of the bug - description: A clear and concise description of what the bug is. - validations: - required: true -- type: textarea - id: command_used - attributes: - label: Command used and terminal output - description: Steps to reproduce the behaviour. Please paste the command you used - to launch the pipeline and the output from your terminal. - render: console - placeholder: '$ nextflow run ... - - - Some output where something broke - - ' -- type: textarea - id: files - attributes: - label: Relevant files - description: 'Please drag and drop the relevant files here. Create a `.zip` archive - if the extension is not allowed. - - Your verbose log file `.nextflow.log` is often useful _(this is a hidden file - in the directory where you launched the pipeline)_ as well as custom Nextflow - configuration files. - - ' -- type: textarea - id: system - attributes: - label: System information - description: '* Nextflow version _(eg. 23.04.0)_ - - * Hardware _(eg. HPC, Desktop, Cloud)_ - - * Executor _(eg. slurm, local, awsbatch)_ - - * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter, Charliecloud, - or Apptainer)_ - - * OS _(eg. CentOS Linux, macOS, Linux Mint)_ - - * Version of plant-food-research-open/assemblyqc _(eg. 1.1, 1.5, 1.8.2)_ - - ' + - type: textarea + id: description + attributes: + label: Description of the bug + description: A clear and concise description of what the bug is. + validations: + required: true + - type: textarea + id: command_used + attributes: + label: Command used and terminal output + description: Steps to reproduce the behaviour. Please paste the command you used + to launch the pipeline and the output from your terminal. + render: console + placeholder: "$ nextflow run ... + + + Some output where something broke + + " + - type: textarea + id: files + attributes: + label: Relevant files + description: "Please drag and drop the relevant files here. Create a `.zip` archive + if the extension is not allowed. + + Your verbose log file `.nextflow.log` is often useful _(this is a hidden file + in the directory where you launched the pipeline)_ as well as custom Nextflow + configuration files. + + " + - type: textarea + id: system + attributes: + label: System information + description: "* Nextflow version _(eg. 23.04.0)_ + + * Hardware _(eg. HPC, Desktop, Cloud)_ + + * Executor _(eg. slurm, local, awsbatch)_ + + * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter, Charliecloud, + or Apptainer)_ + + * OS _(eg. CentOS Linux, macOS, Linux Mint)_ + + * Version of plant-food-research-open/assemblyqc _(eg. 1.1, 1.5, 1.8.2)_ + + " diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 9e9668cc..1e763b3d 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -24,7 +24,7 @@ jobs: strategy: matrix: NXF_VER: - - "23.04.0" + - "22.04.3" - "latest-everything" steps: - name: Check out pipeline code diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 47168689..69574376 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -64,4 +64,4 @@ jobs: env: NXF_SINGULARITY_CACHEDIR: ./ NXF_SINGULARITY_HOME_MOUNT: true - run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results diff --git a/.nf-core.yml b/.nf-core.yml index 98319d1b..61d19b4d 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,23 +1,23 @@ lint: files_exist: - - CODE_OF_CONDUCT.md - - assets/nf-core-assemblyqc_logo_light.png - - docs/images/nf-core-assemblyqc_logo_light.png - - docs/images/nf-core-assemblyqc_logo_dark.png - - .github/ISSUE_TEMPLATE/config.yml - - .github/workflows/awstest.yml - - .github/workflows/awsfulltest.yml + - CODE_OF_CONDUCT.md + - assets/nf-core-assemblyqc_logo_light.png + - docs/images/nf-core-assemblyqc_logo_light.png + - docs/images/nf-core-assemblyqc_logo_dark.png + - .github/ISSUE_TEMPLATE/config.yml + - .github/workflows/awstest.yml + - .github/workflows/awsfulltest.yml files_unchanged: - - CODE_OF_CONDUCT.md - - assets/nf-core-assemblyqc_logo_light.png - - docs/images/nf-core-assemblyqc_logo_light.png - - docs/images/nf-core-assemblyqc_logo_dark.png - - .github/ISSUE_TEMPLATE/bug_report.yml + - CODE_OF_CONDUCT.md + - assets/nf-core-assemblyqc_logo_light.png + - docs/images/nf-core-assemblyqc_logo_light.png + - docs/images/nf-core-assemblyqc_logo_dark.png + - .github/ISSUE_TEMPLATE/bug_report.yml multiqc_config: - - report_comment + - report_comment nextflow_config: - - manifest.name - - manifest.homePage + - manifest.name + - manifest.homePage repository_type: pipeline template: prefix: plant-food-research-open diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index 984321ff..af57081f 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -1,6 +1,6 @@ repos: - repo: https://github.com/pre-commit/mirrors-prettier - rev: "v3.1.1" + rev: "v3.1.0" hooks: - id: prettier - repo: https://github.com/editorconfig-checker/editorconfig-checker.python diff --git a/README.md b/README.md index 92b64574..85f09fe3 100644 --- a/README.md +++ b/README.md @@ -1,7 +1,7 @@ [![GitHub Actions CI Status](https://github.com/plant-food-research-open/assemblyqc/workflows/nf-core%20CI/badge.svg)](https://github.com/plant-food-research-open/assemblyqc/actions?query=workflow%3A%22nf-core+CI%22) [![GitHub Actions Linting Status](https://github.com/plant-food-research-open/assemblyqc/workflows/nf-core%20linting/badge.svg)](https://github.com/plant-food-research-open/assemblyqc/actions?query=workflow%3A%22nf-core+linting%22)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) -[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) +[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A522.04.3-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) diff --git a/assets/assemblysheet.csv b/assets/assemblysheet.csv new file mode 100644 index 00000000..9b200ff9 --- /dev/null +++ b/assets/assemblysheet.csv @@ -0,0 +1,2 @@ +tag,fasta,gff3,monoploid_ids,hic_reads,synteny_labels +FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,,,, diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv deleted file mode 100644 index 5f653ab7..00000000 --- a/assets/samplesheet.csv +++ /dev/null @@ -1,3 +0,0 @@ -sample,fastq_1,fastq_2 -SAMPLE_PAIRED_END,/path/to/fastq/files/AEG588A1_S1_L002_R1_001.fastq.gz,/path/to/fastq/files/AEG588A1_S1_L002_R2_001.fastq.gz -SAMPLE_SINGLE_END,/path/to/fastq/files/AEG588A4_S4_L003_R1_001.fastq.gz, diff --git a/assets/schema_input.json b/assets/schema_input.json index cff17406..6aff8b19 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -7,22 +7,61 @@ "items": { "type": "object", "properties": { - "sample": { + "tag": { "type": "string", - "pattern": "^\\S+$", - "errorMessage": "Sample name must be provided and cannot contain spaces" + "pattern": "^\\w+$", + "errorMessage": "Assembly tags must be provided and can only contain alphanumeric characters including '_'" }, - "fastq_1": { + "fasta": { "type": "string", - "pattern": "^\\S+\\.f(ast)?q\\.gz$", - "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" + "pattern": "^\\S+\\.f(ast|as|sa|na)?\\.gz$", + "errorMessage": "FASTA file path cannot contain spaces and must have extension '.f(ast|as|sa|na)' or '.f(ast|as|sa|na).gz'" }, - "fastq_2": { - "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'", + "gff3": { + "type": "string", + "pattern": "^\\S+\\.gff(3)?\\.gz$", + "errorMessage": "GFF3 file path cannot contain spaces and must have extension '.gff.gz' or '.gff3.gz'" + }, + "monoploid_ids": { + "errorMessage": "Monoploid IDs txt file path cannot contain spaces and must have extension '.txt'", + "anyOf": [ + { + "type": "string", + "pattern": "^\\S+\\.txt$" + }, + { + "type": "string", + "maxLength": 0 + } + ] + }, + "hic_reads": { + "errorMessage": "HiC reads should either be provided as a SRA ID or as a path to paired reads with pattern '*_R{1,2}.(fastq|fq).gz'", + "anyOf": [ + { + "type": "string", + "pattern": "^SR\\w+$" + }, + { + "type": "string", + "pattern": "^\\S+_R\\{1,2\\}\\.f(ast)?q\\.gz$" + }, + { + "type": "string", + "maxLength": 0 + } + ] + }, + "synteny_labels": { + "errorMessage": "Synteny labels tsv path cannot contain spaces and must have extension '.tsv'", "anyOf": [ { "type": "string", - "pattern": "^\\S+\\.f(ast)?q\\.gz$" + "pattern": "^SR\\w+$" + }, + { + "type": "string", + "pattern": "^\\S+_R\\{1,2\\}\\.f(ast)?q\\.gz$" }, { "type": "string", @@ -31,6 +70,6 @@ ] } }, - "required": ["sample", "fastq_1"] + "required": ["tag", "fasta"] } } diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py deleted file mode 100755 index 4a758fe0..00000000 --- a/bin/check_samplesheet.py +++ /dev/null @@ -1,259 +0,0 @@ -#!/usr/bin/env python - - -"""Provide a command line tool to validate and transform tabular samplesheets.""" - - -import argparse -import csv -import logging -import sys -from collections import Counter -from pathlib import Path - -logger = logging.getLogger() - - -class RowChecker: - """ - Define a service that can validate and transform each given row. - - Attributes: - modified (list): A list of dicts, where each dict corresponds to a previously - validated and transformed row. The order of rows is maintained. - - """ - - VALID_FORMATS = ( - ".fq.gz", - ".fastq.gz", - ) - - def __init__( - self, - sample_col="sample", - first_col="fastq_1", - second_col="fastq_2", - single_col="single_end", - **kwargs, - ): - """ - Initialize the row checker with the expected column names. - - Args: - sample_col (str): The name of the column that contains the sample name - (default "sample"). - first_col (str): The name of the column that contains the first (or only) - FASTQ file path (default "fastq_1"). - second_col (str): The name of the column that contains the second (if any) - FASTQ file path (default "fastq_2"). - single_col (str): The name of the new column that will be inserted and - records whether the sample contains single- or paired-end sequencing - reads (default "single_end"). - - """ - super().__init__(**kwargs) - self._sample_col = sample_col - self._first_col = first_col - self._second_col = second_col - self._single_col = single_col - self._seen = set() - self.modified = [] - - def validate_and_transform(self, row): - """ - Perform all validations on the given row and insert the read pairing status. - - Args: - row (dict): A mapping from column headers (keys) to elements of that row - (values). - - """ - self._validate_sample(row) - self._validate_first(row) - self._validate_second(row) - self._validate_pair(row) - self._seen.add((row[self._sample_col], row[self._first_col])) - self.modified.append(row) - - def _validate_sample(self, row): - """Assert that the sample name exists and convert spaces to underscores.""" - if len(row[self._sample_col]) <= 0: - raise AssertionError("Sample input is required.") - # Sanitize samples slightly. - row[self._sample_col] = row[self._sample_col].replace(" ", "_") - - def _validate_first(self, row): - """Assert that the first FASTQ entry is non-empty and has the right format.""" - if len(row[self._first_col]) <= 0: - raise AssertionError("At least the first FASTQ file is required.") - self._validate_fastq_format(row[self._first_col]) - - def _validate_second(self, row): - """Assert that the second FASTQ entry has the right format if it exists.""" - if len(row[self._second_col]) > 0: - self._validate_fastq_format(row[self._second_col]) - - def _validate_pair(self, row): - """Assert that read pairs have the same file extension. Report pair status.""" - if row[self._first_col] and row[self._second_col]: - row[self._single_col] = False - first_col_suffix = Path(row[self._first_col]).suffixes[-2:] - second_col_suffix = Path(row[self._second_col]).suffixes[-2:] - if first_col_suffix != second_col_suffix: - raise AssertionError("FASTQ pairs must have the same file extensions.") - else: - row[self._single_col] = True - - def _validate_fastq_format(self, filename): - """Assert that a given filename has one of the expected FASTQ extensions.""" - if not any(filename.endswith(extension) for extension in self.VALID_FORMATS): - raise AssertionError( - f"The FASTQ file has an unrecognized extension: {filename}\n" - f"It should be one of: {', '.join(self.VALID_FORMATS)}" - ) - - def validate_unique_samples(self): - """ - Assert that the combination of sample name and FASTQ filename is unique. - - In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the - number of times the same sample exist, but with different FASTQ files, e.g., multiple runs per experiment. - - """ - if len(self._seen) != len(self.modified): - raise AssertionError("The pair of sample name and FASTQ must be unique.") - seen = Counter() - for row in self.modified: - sample = row[self._sample_col] - seen[sample] += 1 - row[self._sample_col] = f"{sample}_T{seen[sample]}" - - -def read_head(handle, num_lines=10): - """Read the specified number of lines from the current position in the file.""" - lines = [] - for idx, line in enumerate(handle): - if idx == num_lines: - break - lines.append(line) - return "".join(lines) - - -def sniff_format(handle): - """ - Detect the tabular format. - - Args: - handle (text file): A handle to a `text file`_ object. The read position is - expected to be at the beginning (index 0). - - Returns: - csv.Dialect: The detected tabular format. - - .. _text file: - https://docs.python.org/3/glossary.html#term-text-file - - """ - peek = read_head(handle) - handle.seek(0) - sniffer = csv.Sniffer() - dialect = sniffer.sniff(peek) - return dialect - - -def check_samplesheet(file_in, file_out): - """ - Check that the tabular samplesheet has the structure expected by nf-core pipelines. - - Validate the general shape of the table, expected columns, and each row. Also add - an additional column which records whether one or two FASTQ reads were found. - - Args: - file_in (pathlib.Path): The given tabular samplesheet. The format can be either - CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``. - file_out (pathlib.Path): Where the validated and transformed samplesheet should - be created; always in CSV format. - - Example: - This function checks that the samplesheet follows the following structure, - see also the `viral recon samplesheet`_:: - - sample,fastq_1,fastq_2 - SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz - SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz - SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, - - .. _viral recon samplesheet: - https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv - - """ - required_columns = {"sample", "fastq_1", "fastq_2"} - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_in.open(newline="") as in_handle: - reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle)) - # Validate the existence of the expected header columns. - if not required_columns.issubset(reader.fieldnames): - req_cols = ", ".join(required_columns) - logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.") - sys.exit(1) - # Validate each row. - checker = RowChecker() - for i, row in enumerate(reader): - try: - checker.validate_and_transform(row) - except AssertionError as error: - logger.critical(f"{str(error)} On line {i + 2}.") - sys.exit(1) - checker.validate_unique_samples() - header = list(reader.fieldnames) - header.insert(1, "single_end") - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_out.open(mode="w", newline="") as out_handle: - writer = csv.DictWriter(out_handle, header, delimiter=",") - writer.writeheader() - for row in checker.modified: - writer.writerow(row) - - -def parse_args(argv=None): - """Define and immediately parse command line arguments.""" - parser = argparse.ArgumentParser( - description="Validate and transform a tabular samplesheet.", - epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv", - ) - parser.add_argument( - "file_in", - metavar="FILE_IN", - type=Path, - help="Tabular input samplesheet in CSV or TSV format.", - ) - parser.add_argument( - "file_out", - metavar="FILE_OUT", - type=Path, - help="Transformed output samplesheet in CSV format.", - ) - parser.add_argument( - "-l", - "--log-level", - help="The desired log level (default WARNING).", - choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"), - default="WARNING", - ) - return parser.parse_args(argv) - - -def main(argv=None): - """Coordinate argument parsing and program execution.""" - args = parse_args(argv) - logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s") - if not args.file_in.is_file(): - logger.error(f"The given input file {args.file_in} was not found!") - sys.exit(2) - args.file_out.parent.mkdir(parents=True, exist_ok=True) - check_samplesheet(args.file_in, args.file_out) - - -if __name__ == "__main__": - sys.exit(main()) diff --git a/cleanNXF.sh b/cleanNXF.sh new file mode 100755 index 00000000..8c64a3e0 --- /dev/null +++ b/cleanNXF.sh @@ -0,0 +1,11 @@ +#!/usr/bin/env bash + +rm -rf .nextflow* +echo "Cleaned .nextflow..." +rm -rf .nextflow.pid +echo "Cleaned .nextflow.pid..." +for i in $(ls work | grep -v "conda"); +do + rm -rf "work/$i" +done +echo "Cleaned work..." diff --git a/conf/igenomes.config b/conf/igenomes.config deleted file mode 100644 index 3f114377..00000000 --- a/conf/igenomes.config +++ /dev/null @@ -1,440 +0,0 @@ -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Nextflow config file for iGenomes paths -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Defines reference genomes using iGenome paths. - Can be used by any config that customises the base path using: - $params.igenomes_base / --igenomes_base ----------------------------------------------------------------------------------------- -*/ - -params { - // illumina iGenomes reference file paths - genomes { - 'GRCh37' { - fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed" - } - 'GRCh38' { - fasta = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" - } - 'CHM13' { - fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/BWAIndex/" - bwamem2 = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/BWAmem2Index/" - gtf = "${params.igenomes_base}/Homo_sapiens/NCBI/CHM13/Annotation/Genes/genes.gtf" - gff = "ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/009/914/755/GCF_009914755.1_T2T-CHM13v2.0/GCF_009914755.1_T2T-CHM13v2.0_genomic.gff.gz" - mito_name = "chrM" - } - 'GRCm38' { - fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "1.87e9" - blacklist = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed" - } - 'TAIR10' { - fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt" - mito_name = "Mt" - } - 'EB2' { - fasta = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt" - } - 'UMD3.1' { - fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt" - mito_name = "MT" - } - 'WBcel235' { - fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed" - mito_name = "MtDNA" - macs_gsize = "9e7" - } - 'CanFam3.1' { - fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt" - mito_name = "MT" - } - 'GRCz10' { - fasta = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'BDGP6' { - fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed" - mito_name = "M" - macs_gsize = "1.2e8" - } - 'EquCab2' { - fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt" - mito_name = "MT" - } - 'EB1' { - fasta = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt" - } - 'Galgal4' { - fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'Gm01' { - fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt" - } - 'Mmul_1' { - fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt" - mito_name = "MT" - } - 'IRGSP-1.0' { - fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed" - mito_name = "Mt" - } - 'CHIMP2.1.4' { - fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt" - mito_name = "MT" - } - 'Rnor_5.0' { - fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'Rnor_6.0' { - fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'R64-1-1' { - fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed" - mito_name = "MT" - macs_gsize = "1.2e7" - } - 'EF2' { - fasta = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "1.21e7" - } - 'Sbi1' { - fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt" - } - 'Sscrofa10.2' { - fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt" - mito_name = "MT" - } - 'AGPv3' { - fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed" - mito_name = "Mt" - } - 'hg38' { - fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" - } - 'hg19' { - fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/hg19-blacklist.bed" - } - 'mm10' { - fasta = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "1.87e9" - blacklist = "${projectDir}/assets/blacklists/mm10-blacklist.bed" - } - 'bosTau8' { - fasta = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed" - mito_name = "chrM" - } - 'ce10' { - fasta = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "9e7" - } - 'canFam3' { - fasta = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt" - mito_name = "chrM" - } - 'danRer10' { - fasta = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "1.37e9" - } - 'dm6' { - fasta = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "1.2e8" - } - 'equCab2' { - fasta = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt" - mito_name = "chrM" - } - 'galGal4' { - fasta = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt" - mito_name = "chrM" - } - 'panTro4' { - fasta = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt" - mito_name = "chrM" - } - 'rn6' { - fasta = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed" - mito_name = "chrM" - } - 'sacCer3' { - fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/" - readme = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "1.2e7" - } - 'susScr3' { - fasta = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt" - mito_name = "chrM" - } - } -} diff --git a/conf/modules.config b/conf/modules.config index d91c6aba..bb1fc48d 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -12,24 +12,6 @@ process { - publishDir = [ - path: { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - - withName: SAMPLESHEET_CHECK { - publishDir = [ - path: { "${params.outdir}/pipeline_info" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - - withName: FASTQC { - ext.args = '--quiet' - } - withName: CUSTOM_DUMPSOFTWAREVERSIONS { publishDir = [ path: { "${params.outdir}/pipeline_info" }, @@ -38,13 +20,4 @@ process { ] } - withName: 'MULTIQC' { - ext.args = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' } - publishDir = [ - path: { "${params.outdir}/multiqc" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - } diff --git a/conf/test.config b/conf/test.config index 2bc1ad8e..4e55a764 100644 --- a/conf/test.config +++ b/conf/test.config @@ -19,11 +19,5 @@ params { max_memory = '6.GB' max_time = '6.h' - // Input data - // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets - // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' - - // Genome references - genome = 'R64-1-1' + input = 'https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/assemblysheet.csv' } diff --git a/conf/test_full.config b/conf/test_full.config index 63d2687c..1232442e 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -14,11 +14,5 @@ params { config_profile_name = 'Full test profile' config_profile_description = 'Full test dataset to check pipeline function' - // Input data for full size test - // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) - // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' - - // Genome references - genome = 'R64-1-1' + input = 'https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/assemblysheet.csv' } diff --git a/lib/WorkflowAssemblyqc.groovy b/lib/WorkflowAssemblyqc.groovy index 8be44f94..2c45d1e2 100755 --- a/lib/WorkflowAssemblyqc.groovy +++ b/lib/WorkflowAssemblyqc.groovy @@ -10,113 +10,5 @@ class WorkflowAssemblyqc { // // Check and validate parameters // - public static void initialise(params, log) { - - genomeExistsError(params, log) - - - if (!params.fasta) { - Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." - } - } - - // - // Get workflow summary for MultiQC - // - public static String paramsSummaryMultiqc(workflow, summary) { - String summary_section = '' - for (group in summary.keySet()) { - def group_params = summary.get(group) // This gets the parameters of that particular group - if (group_params) { - summary_section += "

    $group

    \n" - summary_section += "
    \n" - for (param in group_params.keySet()) { - summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" - } - summary_section += "
    \n" - } - } - - String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" - yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" - yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" - yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" - yaml_file_text += "plot_type: 'html'\n" - yaml_file_text += "data: |\n" - yaml_file_text += "${summary_section}" - return yaml_file_text - } - - // - // Generate methods description for MultiQC - // - - public static String toolCitationText(params) { - - // TODO nf-core: Optionally add in-text citation tools to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def citation_text = [ - "Tools used in the workflow included:", - "FastQC (Andrews 2010),", - "MultiQC (Ewels et al. 2016)", - "." - ].join(' ').trim() - - return citation_text - } - - public static String toolBibliographyText(params) { - - // TODO Optionally add bibliographic entries to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def reference_text = [ - "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", - "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " - ].join(' ').trim() - - return reference_text - } - - public static String methodsDescriptionText(run_workflow, mqc_methods_yaml, params) { - // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file - def meta = [:] - meta.workflow = run_workflow.toMap() - meta["manifest_map"] = run_workflow.manifest.toMap() - - // Pipeline DOI - meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" - meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " - - // Tool references - meta["tool_citations"] = "" - meta["tool_bibliography"] = "" - - // TODO Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! - //meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") - //meta["tool_bibliography"] = toolBibliographyText(params) - - - def methods_text = mqc_methods_yaml.text - - def engine = new SimpleTemplateEngine() - def description_html = engine.createTemplate(methods_text).make(meta) - - return description_html - } - - // - // Exit pipeline if incorrect --genome key provided - // - private static void genomeExistsError(params, log) { - if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + - " Currently, the available genome keys are:\n" + - " ${params.genomes.keySet().join(", ")}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - Nextflow.error(error_string) - } - } + public static void initialise(params, log) { } } diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy index b7f82557..abfc7acf 100755 --- a/lib/WorkflowMain.groovy +++ b/lib/WorkflowMain.groovy @@ -11,9 +11,8 @@ class WorkflowMain { // public static String citation(workflow) { return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - // TODO nf-core: Add Zenodo DOI for pipeline after first release - //"* The pipeline\n" + - //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + + "* The pipeline\n" + + " https://doi.org/10.5281/zenodo.10647870\n\n" + "* The nf-core framework\n" + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + "* Software dependencies\n" + @@ -43,11 +42,6 @@ class WorkflowMain { // Check AWS batch settings NfcoreTemplate.awsBatch(workflow, params) - - // Check input has been provided - if (!params.input) { - Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'") - } } // // Get attribute from genome config file e.g. fasta diff --git a/main.nf b/main.nf old mode 100644 new mode 100755 index 42a3a13f..34f2ebb3 --- a/main.nf +++ b/main.nf @@ -9,17 +9,6 @@ nextflow.enable.dsl = 2 -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - GENOME PARAMETER VALUES -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// TODO nf-core: Remove this line if you don't need a FASTA file -// This is an example of how to use getGenomeAttribute() to fetch parameters -// from igenomes.config using `--genome` -params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') - /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ VALIDATE & PRINT PARAMETER SUMMARY @@ -32,18 +21,18 @@ include { validateParameters; paramsHelp } from 'plugin/nf-validation' if (params.help) { def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) def citation = '\n' + WorkflowMain.citation(workflow) + '\n' - def String command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker" + def String command = "nextflow run ${workflow.manifest.name} --input assemblysheet.csv --outdir ./results -profile docker" log.info logo + paramsHelp(command) + citation + NfcoreTemplate.dashedLine(params.monochrome_logs) System.exit(0) } +WorkflowMain.initialise(workflow, params, log) + // Validate input parameters if (params.validate_params) { validateParameters() } -WorkflowMain.initialise(workflow, params, log) - /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ NAMED WORKFLOW FOR PIPELINE diff --git a/modules.json b/modules.json index f3c7519d..f01155f8 100644 --- a/modules.json +++ b/modules.json @@ -7,17 +7,17 @@ "nf-core": { "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e", + "git_sha": "de45447d060b8c8b98575bc637a4a575fd0638e1", "installed_by": ["modules"] }, - "fastqc": { + "fastavalidator": { "branch": "master", - "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", + "git_sha": "89ff95427f695086369d7927a3c17cea2a37a382", "installed_by": ["modules"] }, - "multiqc": { + "gunzip": { "branch": "master", - "git_sha": "4ab13872435962dadc239979554d13709e20bf29", + "git_sha": "3a5fef109d113b4997c9822198664ca5f2716208", "installed_by": ["modules"] } } diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf deleted file mode 100644 index 7a776dbd..00000000 --- a/modules/local/samplesheet_check.nf +++ /dev/null @@ -1,31 +0,0 @@ -process SAMPLESHEET_CHECK { - tag "$samplesheet" - label 'process_single' - - conda "conda-forge::python=3.8.3" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/python:3.8.3' : - 'biocontainers/python:3.8.3' }" - - input: - path samplesheet - - output: - path '*.csv' , emit: csv - path "versions.yml", emit: versions - - when: - task.ext.when == null || task.ext.when - - script: // This script is bundled with the pipeline, in plant-food-research-open/assemblyqc/bin/ - """ - check_samplesheet.py \\ - $samplesheet \\ - samplesheet.valid.csv - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - python: \$(python --version | sed 's/Python //g') - END_VERSIONS - """ -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml index f0c63f69..b48ced26 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.17 + - bioconda::multiqc=1.20 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index 7685b33c..105f9265 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -4,8 +4,8 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : - 'biocontainers/multiqc:1.17--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' : + 'biocontainers/multiqc:1.20--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py index e55b8d43..da033408 100755 --- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py +++ b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py @@ -4,11 +4,10 @@ """Provide functions to merge multiple versions.yml files.""" +import yaml import platform from textwrap import dedent -import yaml - def _make_versions_html(versions): """Generate a tabular HTML output of all versions for MultiQC.""" diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test index eec1db10..b1e1630b 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test @@ -31,7 +31,12 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out).match() } + { assert snapshot( + process.out.versions, + file(process.out.mqc_yml[0]).readLines()[0..10], + file(process.out.yml[0]).readLines()[0..7] + ).match() + } ) } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap index 4274ed57..5f59a936 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap @@ -1,27 +1,33 @@ { "Should run without failures": { "content": [ - { - "0": [ - "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" - ], - "1": [ - "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" - ], - "2": [ - "versions.yml:md5,3843ac526e762117eedf8825b40683df" - ], - "mqc_yml": [ - "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" - ], - "versions": [ - "versions.yml:md5,3843ac526e762117eedf8825b40683df" - ], - "yml": [ - "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" - ] - } + [ + "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" + ], + [ + "data: \"\\n
    Process Name \\", - " \\ Software Version
    CUSTOM_DUMPSOFTWAREVERSIONSpython3.11.7
    yaml5.4.1
    TOOL1tool10.11.9
    TOOL2tool21.9
    WorkflowNextflow12.922000 K (92.984097%)", - "single end (151 cycles)" ] - def log_text = [ "Q20 bases: 12922(92.9841%)", - "reads passed filter: 99" ] - def read_lines = ["@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1", - "TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT", - "AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE - { assert path(process.out.reads.get(0).get(1)).linesGzip.contains(read_line) } - } - }, - { html_text.each { html_part -> - { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } - } - }, - { assert snapshot(process.out.json).match("test_fastp_single_end_json") }, - { log_text.each { log_part -> - { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } - } - }, - { - assert snapshot( - ( - [process.out.reads[0][0].toString()] + // meta - process.out.reads.collect { file(it[1]).getName() } + - process.out.json.collect { file(it[1]).getName() } + - process.out.html.collect { file(it[1]).getName() } + - process.out.log.collect { file(it[1]).getName() } + - process.out.reads_fail.collect { file(it[1]).getName() } + - process.out.reads_merged.collect { file(it[1]).getName() } - ).sort() - ).match("test_fastp_single_end-_match") - }, - { assert snapshot(process.out.versions).match("versions_single_end") } - ) - } - } - - test("test_fastp_single_end-stub") { - - options '-stub' - - when { - params { - outdir = "$outputDir" - } - process { - """ - adapter_fasta = [] - save_trimmed_fail = false - save_merged = false - - input[0] = Channel.of([ - [ id:'test', single_end:true ], - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] - ]) - input[1] = adapter_fasta - input[2] = save_trimmed_fail - input[3] = save_merged - """ - } - } - - then { - - assertAll( - { assert process.success }, - { - assert snapshot( - ( - [process.out.reads[0][0].toString()] + // meta - process.out.reads.collect { file(it[1]).getName() } + - process.out.json.collect { file(it[1]).getName() } + - process.out.html.collect { file(it[1]).getName() } + - process.out.log.collect { file(it[1]).getName() } + - process.out.reads_fail.collect { file(it[1]).getName() } + - process.out.reads_merged.collect { file(it[1]).getName() } - ).sort() - ).match("test_fastp_single_end-for_stub_match") - }, - { assert snapshot(process.out.versions).match("versions_single_end_stub") } - ) - } - } - - test("test_fastp_paired_end") { - - when { - params { - outdir = "$outputDir" - } - process { - """ - adapter_fasta = [] - save_trimmed_fail = false - save_merged = false - - input[0] = Channel.of([ - [ id:'test', single_end:false ], // meta map - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] - ]) - input[1] = adapter_fasta - input[2] = save_trimmed_fail - input[3] = save_merged - """ - } - } - - then { - def html_text = [ "Q20 bases:25.719000 K (93.033098%)", - "The input has little adapter percentage (~0.000000%), probably it's trimmed before."] - def log_text = [ "No adapter detected for read1", - "Q30 bases: 12281(88.3716%)"] - def json_text = ['"passed_filter_reads": 198'] - def read1_lines = ["@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1", - "TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT", - "AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE - { assert path(process.out.reads.get(0).get(1).get(0)).linesGzip.contains(read1_line) } - } - }, - { read2_lines.each { read2_line -> - { assert path(process.out.reads.get(0).get(1).get(1)).linesGzip.contains(read2_line) } - } - }, - { html_text.each { html_part -> - { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } - } - }, - { json_text.each { json_part -> - { assert path(process.out.json.get(0).get(1)).getText().contains(json_part) } - } - }, - { log_text.each { log_part -> - { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } - } - }, - { - assert snapshot( - ( - [process.out.reads[0][0].toString()] + // meta - process.out.reads.collect { it[1].collect { item -> file(item).getName() } } + - process.out.json.collect { file(it[1]).getName() } + - process.out.html.collect { file(it[1]).getName() } + - process.out.log.collect { file(it[1]).getName() } + - process.out.reads_fail.collect { file(it[1]).getName() } + - process.out.reads_merged.collect { file(it[1]).getName() } - ).sort() - ).match("test_fastp_paired_end_match") - }, - { assert snapshot(process.out.versions).match("versions_paired_end") } - ) - } - } - - test("test_fastp_paired_end-stub") { - - options '-stub' - - when { - params { - outdir = "$outputDir" - } - process { - """ - adapter_fasta = [] - save_trimmed_fail = false - save_merged = false - - input[0] = Channel.of([ - [ id:'test', single_end:false ], // meta map - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] - ]) - input[1] = adapter_fasta - input[2] = save_trimmed_fail - input[3] = save_merged - """ - } - } - - then { - assertAll( - { assert process.success }, - { - assert snapshot( - ( - [process.out.reads[0][0].toString()] + // meta - process.out.reads.collect { it[1].collect { item -> file(item).getName() } } + - process.out.json.collect { file(it[1]).getName() } + - process.out.html.collect { file(it[1]).getName() } + - process.out.log.collect { file(it[1]).getName() } + - process.out.reads_fail.collect { file(it[1]).getName() } + - process.out.reads_merged.collect { file(it[1]).getName() } - ).sort() - ).match("test_fastp_paired_end-for_stub_match") - }, - { assert snapshot(process.out.versions).match("versions_paired_end-stub") } - ) - } - } - - test("fastp test_fastp_interleaved") { - config './nextflow.config' - when { - params { - outdir = "$outputDir" - } - process { - """ - adapter_fasta = [] - save_trimmed_fail = false - save_merged = false - - input[0] = Channel.of([ - [ id:'test', single_end:true ], // meta map - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) ] - ]) - input[1] = adapter_fasta - input[2] = save_trimmed_fail - input[3] = save_merged - """ - } - } - - then { - def html_text = [ "Q20 bases:25.719000 K (93.033098%)", - "paired end (151 cycles + 151 cycles)"] - def log_text = [ "Q20 bases: 12922(92.9841%)", - "reads passed filter: 198"] - def read_lines = [ "@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1", - "TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT", - "AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE - { assert path(process.out.reads.get(0).get(1)).linesGzip.contains(read_line) } - } - }, - { html_text.each { html_part -> - { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } - } - }, - { assert snapshot(process.out.json).match("fastp test_fastp_interleaved_json") }, - { log_text.each { log_part -> - { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } - } - }, - { - assert snapshot( - ( - [process.out.reads[0][0].toString()] + // meta - process.out.reads.collect { file(it[1]).getName() } + - process.out.json.collect { file(it[1]).getName() } + - process.out.html.collect { file(it[1]).getName() } + - process.out.log.collect { file(it[1]).getName() } + - process.out.reads_fail.collect { file(it[1]).getName() } + - process.out.reads_merged.collect { file(it[1]).getName() } - ).sort() - ).match("test_fastp_interleaved-_match") - }, - { assert snapshot(process.out.versions).match("versions_interleaved") } - ) - } - } - - test("fastp test_fastp_interleaved-stub") { - - options '-stub' - - config './nextflow.config' - when { - params { - outdir = "$outputDir" - } - process { - """ - adapter_fasta = [] - save_trimmed_fail = false - save_merged = false - - input[0] = Channel.of([ - [ id:'test', single_end:true ], // meta map - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) ] - ]) - input[1] = adapter_fasta - input[2] = save_trimmed_fail - input[3] = save_merged - """ - } - } - - then { - assertAll( - { assert process.success }, - { - assert snapshot( - ( - [process.out.reads[0][0].toString()] + // meta - process.out.reads.collect { file(it[1]).getName() } + - process.out.json.collect { file(it[1]).getName() } + - process.out.html.collect { file(it[1]).getName() } + - process.out.log.collect { file(it[1]).getName() } + - process.out.reads_fail.collect { file(it[1]).getName() } + - process.out.reads_merged.collect { file(it[1]).getName() } - ).sort() - ).match("test_fastp_interleaved-for_stub_match") - }, - { assert snapshot(process.out.versions).match("versions_interleaved-stub") } - ) - } - } - - test("test_fastp_single_end_trim_fail") { - - when { - params { - outdir = "$outputDir" - } - process { - """ - adapter_fasta = [] - save_trimmed_fail = true - save_merged = false - - input[0] = Channel.of([ - [ id:'test', single_end:true ], // meta map - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] - ]) - input[1] = adapter_fasta - input[2] = save_trimmed_fail - input[3] = save_merged - """ - } - } - - then { - def html_text = [ "Q20 bases:12.922000 K (92.984097%)", - "single end (151 cycles)"] - def log_text = [ "Q20 bases: 12922(92.9841%)", - "reads passed filter: 99" ] - def read_lines = [ "@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1", - "TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT", - "AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE - { assert path(process.out.reads.get(0).get(1)).linesGzip.contains(read_line) } - } - }, - { failed_read_lines.each { failed_read_line -> - { assert path(process.out.reads_fail.get(0).get(1)).linesGzip.contains(failed_read_line) } - } - }, - { html_text.each { html_part -> - { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } - } - }, - { assert snapshot(process.out.json).match("test_fastp_single_end_trim_fail_json") }, - { log_text.each { log_part -> - { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } - } - }, - { assert snapshot(process.out.versions).match("versions_single_end_trim_fail") } - ) - } - } - - test("test_fastp_paired_end_trim_fail") { - - when { - params { - outdir = "$outputDir" - } - process { - """ - adapter_fasta = [] - save_trimmed_fail = true - save_merged = false - - input[0] = Channel.of([ - [ id:'test', single_end:false ], // meta map - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)] - ]) - input[1] = adapter_fasta - input[2] = save_trimmed_fail - input[3] = save_merged - """ - } - } - - then { - def html_text = [ "Q20 bases:25.719000 K (93.033098%)", - "The input has little adapter percentage (~0.000000%), probably it's trimmed before."] - def log_text = [ "No adapter detected for read1", - "Q30 bases: 12281(88.3716%)"] - def json_text = ['"passed_filter_reads": 198'] - def read1_lines = ["@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1", - "TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT", - "AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE - { assert path(process.out.reads.get(0).get(1).get(0)).linesGzip.contains(read1_line) } - } - }, - { read2_lines.each { read2_line -> - { assert path(process.out.reads.get(0).get(1).get(1)).linesGzip.contains(read2_line) } - } - }, - { failed_read2_lines.each { failed_read2_line -> - { assert path(process.out.reads_fail.get(0).get(1).get(1)).linesGzip.contains(failed_read2_line) } - } - }, - { html_text.each { html_part -> - { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } - } - }, - { json_text.each { json_part -> - { assert path(process.out.json.get(0).get(1)).getText().contains(json_part) } - } - }, - { log_text.each { log_part -> - { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } - } - }, - { assert snapshot(process.out.versions).match("versions_paired_end_trim_fail") } - ) - } - } - - test("test_fastp_paired_end_merged") { - - when { - params { - outdir = "$outputDir" - } - process { - """ - adapter_fasta = [] - save_trimmed_fail = false - save_merged = true - input[0] = Channel.of([ - [ id:'test', single_end:false ], // meta map - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] - ]) - input[1] = adapter_fasta - input[2] = save_trimmed_fail - input[3] = save_merged - """ - } - } - - then { - def html_text = [ "
    "] - def log_text = [ "Merged and filtered:", - "total reads: 75", - "total bases: 13683"] - def json_text = ['"merged_and_filtered": {', '"total_reads": 75', '"total_bases": 13683'] - def read1_lines = [ "@ERR5069949.1066259 NS500628:121:HK3MMAFX2:1:11312:18369:8333/1", - "CCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTC", - "AAAAAEAEEAEEEEEEEEEEEEEEEEAEEEEAEEEEEEEEAEEEEEEEEEEEEEEEEE/EAEEEEEE/6EEEEEEEEEEAEEAEEE/EE/AEEAEEEEEAEEEA/EEAAEAE - { assert path(process.out.reads.get(0).get(1).get(0)).linesGzip.contains(read1_line) } - } - }, - { read2_lines.each { read2_line -> - { assert path(process.out.reads.get(0).get(1).get(1)).linesGzip.contains(read2_line) } - } - }, - { read_merged_lines.each { read_merged_line -> - { assert path(process.out.reads_merged.get(0).get(1)).linesGzip.contains(read_merged_line) } - } - }, - { html_text.each { html_part -> - { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } - } - }, - { json_text.each { json_part -> - { assert path(process.out.json.get(0).get(1)).getText().contains(json_part) } - } - }, - { log_text.each { log_part -> - { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } - } - }, - { - assert snapshot( - ( - [process.out.reads[0][0].toString()] + // meta - process.out.reads.collect { it[1].collect { item -> file(item).getName() } } + - process.out.json.collect { file(it[1]).getName() } + - process.out.html.collect { file(it[1]).getName() } + - process.out.log.collect { file(it[1]).getName() } + - process.out.reads_fail.collect { file(it[1]).getName() } + - process.out.reads_merged.collect { file(it[1]).getName() } - ).sort() - ).match("test_fastp_paired_end_merged_match") - }, - { assert snapshot(process.out.versions).match("versions_paired_end_merged") } - ) - } - } - - test("test_fastp_paired_end_merged-stub") { - - options '-stub' - - when { - params { - outdir = "$outputDir" - } - process { - """ - adapter_fasta = [] - save_trimmed_fail = false - save_merged = true - - input[0] = Channel.of([ - [ id:'test', single_end:false ], // meta map - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] - ]) - input[1] = adapter_fasta - input[2] = save_trimmed_fail - input[3] = save_merged - """ - } - } - - then { - assertAll( - { assert process.success }, - { - assert snapshot( - ( - [process.out.reads[0][0].toString()] + // meta - process.out.reads.collect { it[1].collect { item -> file(item).getName() } } + - process.out.json.collect { file(it[1]).getName() } + - process.out.html.collect { file(it[1]).getName() } + - process.out.log.collect { file(it[1]).getName() } + - process.out.reads_fail.collect { file(it[1]).getName() } + - process.out.reads_merged.collect { file(it[1]).getName() } - ).sort() - ).match("test_fastp_paired_end_merged-for_stub_match") - }, - { assert snapshot(process.out.versions).match("versions_paired_end_merged_stub") } - ) - } - } - - test("test_fastp_paired_end_merged_adapterlist") { - - when { - params { - outdir = "$outputDir" - } - process { - """ - adapter_fasta = Channel.of([ file(params.modules_testdata_base_path + 'delete_me/fastp/adapters.fasta', checkIfExists: true) ]) - save_trimmed_fail = false - save_merged = true - - input[0] = Channel.of([ - [ id:'test', single_end:false ], // meta map - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] - ]) - input[1] = adapter_fasta - input[2] = save_trimmed_fail - input[3] = save_merged - """ - } - } - - then { - def html_text = [ "
    "] - def log_text = [ "Merged and filtered:", - "total reads: 75", - "total bases: 13683"] - def json_text = ['"merged_and_filtered": {', '"total_reads": 75', '"total_bases": 13683',"--adapter_fasta"] - def read1_lines = ["@ERR5069949.1066259 NS500628:121:HK3MMAFX2:1:11312:18369:8333/1", - "CCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTC", - "AAAAAEAEEAEEEEEEEEEEEEEEEEAEEEEAEEEEEEEEAEEEEEEEEEEEEEEEEE/EAEEEEEE/6EEEEEEEEEEAEEAEEE/EE/AEEAEEEEEAEEEA/EEAAEAE - { assert path(process.out.reads.get(0).get(1).get(0)).linesGzip.contains(read1_line) } - } - }, - { read2_lines.each { read2_line -> - { assert path(process.out.reads.get(0).get(1).get(1)).linesGzip.contains(read2_line) } - } - }, - { read_merged_lines.each { read_merged_line -> - { assert path(process.out.reads_merged.get(0).get(1)).linesGzip.contains(read_merged_line) } - } - }, - { html_text.each { html_part -> - { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } - } - }, - { json_text.each { json_part -> - { assert path(process.out.json.get(0).get(1)).getText().contains(json_part) } - } - }, - { log_text.each { log_part -> - { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } - } - }, - { assert snapshot(process.out.versions).match("versions_paired_end_merged_adapterlist") } - ) - } - } -} \ No newline at end of file diff --git a/modules/nf-core/fastp/tests/main.nf.test.snap b/modules/nf-core/fastp/tests/main.nf.test.snap deleted file mode 100644 index b4c0e1dd..00000000 --- a/modules/nf-core/fastp/tests/main.nf.test.snap +++ /dev/null @@ -1,330 +0,0 @@ -{ - "fastp test_fastp_interleaved_json": { - "content": [ - [ - [ - { - "id": "test", - "single_end": true - }, - "test.fastp.json:md5,168f516f7bd4b7b6c32da7cba87299a4" - ] - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-17T18:08:06.123035" - }, - "test_fastp_paired_end_merged-for_stub_match": { - "content": [ - [ - [ - "test_1.fastp.fastq.gz", - "test_2.fastp.fastq.gz" - ], - "test.fastp.html", - "test.fastp.json", - "test.fastp.log", - "test.merged.fastq.gz", - "{id=test, single_end=false}" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-17T18:10:13.467574" - }, - "versions_interleaved": { - "content": [ - [ - "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-01T11:56:24.615634793" - }, - "test_fastp_single_end_json": { - "content": [ - [ - [ - { - "id": "test", - "single_end": true - }, - "test.fastp.json:md5,c852d7a6dba5819e4ac8d9673bedcacc" - ] - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-17T18:06:00.223817" - }, - "versions_paired_end": { - "content": [ - [ - "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-01T11:55:42.333545689" - }, - "test_fastp_paired_end_match": { - "content": [ - [ - [ - "test_1.fastp.fastq.gz", - "test_2.fastp.fastq.gz" - ], - "test.fastp.html", - "test.fastp.json", - "test.fastp.log", - "{id=test, single_end=false}" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-01T12:03:06.431833729" - }, - "test_fastp_interleaved-_match": { - "content": [ - [ - "test.fastp.fastq.gz", - "test.fastp.html", - "test.fastp.json", - "test.fastp.log", - "{id=test, single_end=true}" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-01T12:03:37.827323085" - }, - "test_fastp_paired_end_merged_match": { - "content": [ - [ - [ - "test_1.fastp.fastq.gz", - "test_2.fastp.fastq.gz" - ], - "test.fastp.html", - "test.fastp.json", - "test.fastp.log", - "test.merged.fastq.gz", - "{id=test, single_end=false}" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-01T12:08:44.496251446" - }, - "versions_single_end_stub": { - "content": [ - [ - "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-01T11:55:27.354051299" - }, - "versions_interleaved-stub": { - "content": [ - [ - "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-01T11:56:46.535528418" - }, - "versions_single_end_trim_fail": { - "content": [ - [ - "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-01T11:59:03.724591407" - }, - "test_fastp_paired_end-for_stub_match": { - "content": [ - [ - [ - "test_1.fastp.fastq.gz", - "test_2.fastp.fastq.gz" - ], - "test.fastp.html", - "test.fastp.json", - "test.fastp.log", - "{id=test, single_end=false}" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-17T18:07:15.398827" - }, - "versions_paired_end-stub": { - "content": [ - [ - "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-01T11:56:06.50017282" - }, - "versions_single_end": { - "content": [ - [ - "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-01T11:55:07.67921647" - }, - "versions_paired_end_merged_stub": { - "content": [ - [ - "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-01T11:59:47.350653154" - }, - "test_fastp_interleaved-for_stub_match": { - "content": [ - [ - "test.fastp.fastq.gz", - "test.fastp.html", - "test.fastp.json", - "test.fastp.log", - "{id=test, single_end=true}" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-17T18:08:06.127974" - }, - "versions_paired_end_trim_fail": { - "content": [ - [ - "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-01T11:59:18.140484878" - }, - "test_fastp_single_end-for_stub_match": { - "content": [ - [ - "test.fastp.fastq.gz", - "test.fastp.html", - "test.fastp.json", - "test.fastp.log", - "{id=test, single_end=true}" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-17T18:06:00.244202" - }, - "test_fastp_single_end-_match": { - "content": [ - [ - "test.fastp.fastq.gz", - "test.fastp.html", - "test.fastp.json", - "test.fastp.log", - "{id=test, single_end=true}" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-01T11:57:30.791982648" - }, - "versions_paired_end_merged_adapterlist": { - "content": [ - [ - "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-01T12:05:37.845370554" - }, - "versions_paired_end_merged": { - "content": [ - [ - "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-01T11:59:32.860543858" - }, - "test_fastp_single_end_trim_fail_json": { - "content": [ - [ - [ - { - "id": "test", - "single_end": true - }, - "test.fastp.json:md5,9a7ee180f000e8d00c7fb67f06293eb5" - ] - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-17T18:08:41.942317" - } -} \ No newline at end of file diff --git a/modules/nf-core/fastp/tests/nextflow.config b/modules/nf-core/fastp/tests/nextflow.config deleted file mode 100644 index 0f7849ad..00000000 --- a/modules/nf-core/fastp/tests/nextflow.config +++ /dev/null @@ -1,6 +0,0 @@ -process { - - withName: FASTP { - ext.args = "--interleaved_in" - } -} diff --git a/modules/nf-core/fastp/tests/tags.yml b/modules/nf-core/fastp/tests/tags.yml deleted file mode 100644 index c1afcce7..00000000 --- a/modules/nf-core/fastp/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -fastp: - - modules/nf-core/fastp/** diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf index e89d2c47..9e19a74c 100644 --- a/modules/nf-core/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -3,9 +3,9 @@ process FASTQC { label 'process_medium' conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0' : - 'quay.io/biocontainers/fastqc:0.12.1--hdfd78af_0' }" + 'biocontainers/fastqc:0.12.1--hdfd78af_0' }" input: tuple val(meta), path(reads) diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index 31a21314..b9e8f926 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -3,20 +3,24 @@ nextflow_process { name "Test Process FASTQC" script "../main.nf" process "FASTQC" - tag "modules" tag "modules_nfcore" tag "fastqc" - test("sarscov2 single-end [fastq]") { + test("Single-Read") { when { + params { + outdir = "$outputDir" + } process { """ - input[0] = Channel.of([ + input[0] = [ [ id: 'test', single_end:true ], - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] - ]) + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + ] """ } } @@ -24,189 +28,82 @@ nextflow_process { then { assertAll ( { assert process.success }, - // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. // looks like this:
    Mon 2 Oct 2023
    test.gz
    // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 - - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("
    File typeConventional base calls
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    \\n \\n \\n \\n \\n \\n \\n \\n\\", + " \\n\\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n \\n \\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n\\n\\n \\n\\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\" + ], + [ + "CUSTOM_DUMPSOFTWAREVERSIONS:", + " python: 3.11.7", + " yaml: 5.4.1", + "TOOL1:", + " tool1: 0.11.9", + "TOOL2:", + " tool2: '1.9'", + "Workflow:" + ] ], - "timestamp": "2023-11-03T14:43:22.157011" + "timestamp": "2024-01-09T23:01:18.710682" } -} +} \ No newline at end of file diff --git a/modules/nf-core/fastavalidator/environment.yml b/modules/nf-core/fastavalidator/environment.yml new file mode 100644 index 00000000..70f346ef --- /dev/null +++ b/modules/nf-core/fastavalidator/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "fastavalidator" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::py_fasta_validator=0.6" diff --git a/modules/nf-core/fastavalidator/main.nf b/modules/nf-core/fastavalidator/main.nf new file mode 100644 index 00000000..ac5470fb --- /dev/null +++ b/modules/nf-core/fastavalidator/main.nf @@ -0,0 +1,62 @@ +process FASTAVALIDATOR { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/py_fasta_validator:0.6--py37h595c7a6_0': + 'biocontainers/py_fasta_validator:0.6--py37h595c7a6_0' }" + + input: + tuple val(meta), path(fasta) + + output: + tuple val(meta), path('*.success.log') , emit: success_log , optional: true + tuple val(meta), path('*.error.log') , emit: error_log , optional: true + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + py_fasta_validator \\ + -f $fasta \\ + 2> "${prefix}.error.log" \\ + || echo "Errors from fasta_validate printed to ${prefix}.error.log" + + if [ \$(cat "${prefix}.error.log" | wc -l) -gt 0 ]; then + echo "Validation failed..." + + cat \\ + "${prefix}.error.log" + else + echo "Validation successful..." + + mv \\ + "${prefix}.error.log" \\ + fasta_validate.stderr + + echo "Validation successful..." \\ + > "${prefix}.success.log" + fi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + py_fasta_validator: \$(py_fasta_validator -v | sed 's/.* version //') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + echo "Validation successful..." \\ + > "${prefix}.success.log" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + py_fasta_validator: \$(py_fasta_validator -v | sed 's/.* version //') + END_VERSIONS + """ +} diff --git a/modules/nf-core/fastavalidator/meta.yml b/modules/nf-core/fastavalidator/meta.yml new file mode 100644 index 00000000..c5c4371c --- /dev/null +++ b/modules/nf-core/fastavalidator/meta.yml @@ -0,0 +1,53 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "fastavalidator" +description: | + "Python C-extension for a simple validator for fasta files. The module emits the validated file or an + error log upon validation failure." +keywords: + - fasta + - validation + - genome +tools: + - fasta_validate: + description: | + "Python C-extension for a simple C code to validate a fasta file. It only checks a few things, + and by default only sets its response via the return code, + so you will need to check that!" + homepage: "https://github.com/linsalrob/py_fasta_validator" + documentation: "https://github.com/linsalrob/py_fasta_validator" + tool_dev_url: "https://github.com/linsalrob/py_fasta_validator" + doi: "10.5281/zenodo.5002710" + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing file information + e.g. [ id:'test' ] + - fasta: + type: file + description: Input fasta file + pattern: "*.fasta" +output: + - meta: + type: map + description: | + Groovy Map containing file information + e.g. [ id:'test' ] + - success_log: + type: file + description: Log file for successful validation + pattern: "*.success.log" + - error_log: + type: file + description: Log file for failed validation + pattern: "*.error.log" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@gallvp" +maintainers: + - "@gallvp" diff --git a/modules/nf-core/fastavalidator/tests/main.nf.test b/modules/nf-core/fastavalidator/tests/main.nf.test new file mode 100644 index 00000000..bb8c22cf --- /dev/null +++ b/modules/nf-core/fastavalidator/tests/main.nf.test @@ -0,0 +1,60 @@ +nextflow_process { + + name "Test Process FASTAVALIDATOR" + script "../main.nf" + process "FASTAVALIDATOR" + + tag "modules" + tag "modules_nfcore" + tag "fastavalidator" + + test("sarscov2-fasta-valid") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.success_log != null }, + { assert process.out.error_log == [] }, + { assert path(process.out.success_log.get(0).get(1)).getText().contains("Validation successful...") } + ) + } + + } + + test("sarscov2-gff3-invalid") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.success_log == [] }, + { assert process.out.error_log != null }, + { assert path(process.out.error_log.get(0).get(1)).getText().contains("genome.gff3 does not start with a >") } + ) + } + + } +} diff --git a/modules/nf-core/fastavalidator/tests/main.nf.test.snap b/modules/nf-core/fastavalidator/tests/main.nf.test.snap new file mode 100644 index 00000000..382dee72 --- /dev/null +++ b/modules/nf-core/fastavalidator/tests/main.nf.test.snap @@ -0,0 +1,76 @@ +{ + "sarscov2-fasta-valid": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.success.log:md5,b0b859eda1db5cd43915846e00ebc22c" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,05aa059840b3b4dd6d88bc1e4bf976d7" + ], + "error_log": [ + + ], + "success_log": [ + [ + { + "id": "test", + "single_end": false + }, + "test.success.log:md5,b0b859eda1db5cd43915846e00ebc22c" + ] + ], + "versions": [ + "versions.yml:md5,05aa059840b3b4dd6d88bc1e4bf976d7" + ] + } + ], + "timestamp": "2023-11-28T11:23:25.106872" + }, + "sarscov2-gff3-invalid": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test.error.log:md5,531d520c0e7767176f743f197f1f87b3" + ] + ], + "2": [ + "versions.yml:md5,05aa059840b3b4dd6d88bc1e4bf976d7" + ], + "error_log": [ + [ + { + "id": "test", + "single_end": false + }, + "test.error.log:md5,531d520c0e7767176f743f197f1f87b3" + ] + ], + "success_log": [ + + ], + "versions": [ + "versions.yml:md5,05aa059840b3b4dd6d88bc1e4bf976d7" + ] + } + ], + "timestamp": "2023-11-28T11:23:29.40324" + } +} \ No newline at end of file diff --git a/modules/nf-core/fastavalidator/tests/tags.yml b/modules/nf-core/fastavalidator/tests/tags.yml new file mode 100644 index 00000000..c3c77576 --- /dev/null +++ b/modules/nf-core/fastavalidator/tests/tags.yml @@ -0,0 +1,2 @@ +fastavalidator: + - "modules/nf-core/fastavalidator/**" diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf deleted file mode 100644 index 9e19a74c..00000000 --- a/modules/nf-core/fastqc/main.nf +++ /dev/null @@ -1,55 +0,0 @@ -process FASTQC { - tag "$meta.id" - label 'process_medium' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0' : - 'biocontainers/fastqc:0.12.1--hdfd78af_0' }" - - input: - tuple val(meta), path(reads) - - output: - tuple val(meta), path("*.html"), emit: html - tuple val(meta), path("*.zip") , emit: zip - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - // Make list of old name and new name pairs to use for renaming in the bash while loop - def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] } - def rename_to = old_new_pairs*.join(' ').join(' ') - def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ') - """ - printf "%s %s\\n" $rename_to | while read old_name new_name; do - [ -f "\${new_name}" ] || ln -s \$old_name \$new_name - done - - fastqc \\ - $args \\ - --threads $task.cpus \\ - $renamed_files - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.html - touch ${prefix}.zip - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) - END_VERSIONS - """ -} diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml deleted file mode 100644 index ee5507e0..00000000 --- a/modules/nf-core/fastqc/meta.yml +++ /dev/null @@ -1,57 +0,0 @@ -name: fastqc -description: Run FastQC on sequenced reads -keywords: - - quality control - - qc - - adapters - - fastq -tools: - - fastqc: - description: | - FastQC gives general quality metrics about your reads. - It provides information about the quality score distribution - across your reads, the per base sequence content (%A/C/G/T). - You get information about adapter contamination and other - overrepresented sequences. - homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ - documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/ - licence: ["GPL-2.0-only"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: | - List of input FastQ files of size 1 and 2 for single-end and paired-end data, - respectively. -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - html: - type: file - description: FastQC report - pattern: "*_{fastqc.html}" - - zip: - type: file - description: FastQC report archive - pattern: "*_{fastqc.zip}" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@drpatelh" - - "@grst" - - "@ewels" - - "@FelixKrueger" -maintainers: - - "@drpatelh" - - "@grst" - - "@ewels" - - "@FelixKrueger" diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test deleted file mode 100644 index b9e8f926..00000000 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ /dev/null @@ -1,109 +0,0 @@ -nextflow_process { - - name "Test Process FASTQC" - script "../main.nf" - process "FASTQC" - tag "modules" - tag "modules_nfcore" - tag "fastqc" - - test("Single-Read") { - - when { - params { - outdir = "$outputDir" - } - process { - """ - input[0] = [ - [ id: 'test', single_end:true ], - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] - ] - """ - } - } - - then { - assertAll ( - { assert process.success }, - // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. - // looks like this:
    Mon 2 Oct 2023
    test.gz
    - // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 - { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" }, - { assert path(process.out.html.get(0).get(1)).getText().contains("") }, - { assert snapshot(process.out.versions).match("versions") }, - { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" } - ) - } - } -// TODO -// // -// // Test with paired-end data -// // -// workflow test_fastqc_paired_end { -// input = [ -// [id: 'test', single_end: false], // meta map -// [ -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) -// ] -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with interleaved data -// // -// workflow test_fastqc_interleaved { -// input = [ -// [id: 'test', single_end: false], // meta map -// file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with bam data -// // -// workflow test_fastqc_bam { -// input = [ -// [id: 'test', single_end: false], // meta map -// file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with multiple samples -// // -// workflow test_fastqc_multiple { -// input = [ -// [id: 'test', single_end: false], // meta map -// [ -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) -// ] -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with custom prefix -// // -// workflow test_fastqc_custom_prefix { -// input = [ -// [ id:'mysample', single_end:true ], // meta map -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } -} diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap deleted file mode 100644 index 636a32ce..00000000 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ /dev/null @@ -1,10 +0,0 @@ -{ - "versions": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "timestamp": "2023-10-09T23:40:54+0000" - } -} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/tags.yml b/modules/nf-core/fastqc/tests/tags.yml deleted file mode 100644 index 7834294b..00000000 --- a/modules/nf-core/fastqc/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -fastqc: - - modules/nf-core/fastqc/** diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/gunzip/environment.yml similarity index 61% rename from modules/nf-core/fastqc/environment.yml rename to modules/nf-core/gunzip/environment.yml index 1787b38a..25910b34 100644 --- a/modules/nf-core/fastqc/environment.yml +++ b/modules/nf-core/gunzip/environment.yml @@ -1,7 +1,7 @@ -name: fastqc +name: gunzip channels: - conda-forge - bioconda - defaults dependencies: - - bioconda::fastqc=0.12.1 + - conda-forge::sed=4.7 diff --git a/modules/nf-core/gunzip/main.nf b/modules/nf-core/gunzip/main.nf new file mode 100644 index 00000000..468a6f28 --- /dev/null +++ b/modules/nf-core/gunzip/main.nf @@ -0,0 +1,48 @@ +process GUNZIP { + tag "$archive" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : + 'nf-core/ubuntu:20.04' }" + + input: + tuple val(meta), path(archive) + + output: + tuple val(meta), path("$gunzip"), emit: gunzip + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + gunzip = archive.toString() - '.gz' + """ + # Not calling gunzip itself because it creates files + # with the original group ownership rather than the + # default one for that user / the work directory + gzip \\ + -cd \\ + $args \\ + $archive \\ + > $gunzip + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//') + END_VERSIONS + """ + + stub: + gunzip = archive.toString() - '.gz' + """ + touch $gunzip + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/gunzip/meta.yml b/modules/nf-core/gunzip/meta.yml new file mode 100644 index 00000000..231034f2 --- /dev/null +++ b/modules/nf-core/gunzip/meta.yml @@ -0,0 +1,39 @@ +name: gunzip +description: Compresses and decompresses files. +keywords: + - gunzip + - compression + - decompression +tools: + - gunzip: + description: | + gzip is a file format and a software application used for file compression and decompression. + documentation: https://www.gnu.org/software/gzip/manual/gzip.html + licence: ["GPL-3.0-or-later"] +input: + - meta: + type: map + description: | + Optional groovy Map containing meta information + e.g. [ id:'test', single_end:false ] + - archive: + type: file + description: File to be compressed/uncompressed + pattern: "*.*" +output: + - gunzip: + type: file + description: Compressed/uncompressed file + pattern: "*.*" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@joseespinosa" + - "@drpatelh" + - "@jfy133" +maintainers: + - "@joseespinosa" + - "@drpatelh" + - "@jfy133" diff --git a/modules/nf-core/gunzip/tests/main.nf.test b/modules/nf-core/gunzip/tests/main.nf.test new file mode 100644 index 00000000..6406008e --- /dev/null +++ b/modules/nf-core/gunzip/tests/main.nf.test @@ -0,0 +1,36 @@ +nextflow_process { + + name "Test Process GUNZIP" + script "../main.nf" + process "GUNZIP" + tag "gunzip" + tag "modules_nfcore" + tag "modules" + + test("Should run without failures") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([ + [], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ] + ) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/gunzip/tests/main.nf.test.snap b/modules/nf-core/gunzip/tests/main.nf.test.snap new file mode 100644 index 00000000..720fd9ff --- /dev/null +++ b/modules/nf-core/gunzip/tests/main.nf.test.snap @@ -0,0 +1,31 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + [ + [ + + ], + "test_1.fastq:md5,4161df271f9bfcd25d5845a1e220dbec" + ] + ], + "1": [ + "versions.yml:md5,54376d32aca20e937a4ec26dac228e84" + ], + "gunzip": [ + [ + [ + + ], + "test_1.fastq:md5,4161df271f9bfcd25d5845a1e220dbec" + ] + ], + "versions": [ + "versions.yml:md5,54376d32aca20e937a4ec26dac228e84" + ] + } + ], + "timestamp": "2023-10-17T15:35:37.690477896" + } +} \ No newline at end of file diff --git a/modules/nf-core/gunzip/tests/tags.yml b/modules/nf-core/gunzip/tests/tags.yml new file mode 100644 index 00000000..fd3f6915 --- /dev/null +++ b/modules/nf-core/gunzip/tests/tags.yml @@ -0,0 +1,2 @@ +gunzip: + - modules/nf-core/gunzip/** diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml deleted file mode 100644 index bc0bdb5b..00000000 --- a/modules/nf-core/multiqc/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: multiqc -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::multiqc=1.18 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf deleted file mode 100644 index 00cc48d2..00000000 --- a/modules/nf-core/multiqc/main.nf +++ /dev/null @@ -1,55 +0,0 @@ -process MULTIQC { - label 'process_single' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.18--pyhdfd78af_0' : - 'biocontainers/multiqc:1.18--pyhdfd78af_0' }" - - input: - path multiqc_files, stageAs: "?/*" - path(multiqc_config) - path(extra_multiqc_config) - path(multiqc_logo) - - output: - path "*multiqc_report.html", emit: report - path "*_data" , emit: data - path "*_plots" , optional:true, emit: plots - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def config = multiqc_config ? "--config $multiqc_config" : '' - def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : '' - def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : '' - """ - multiqc \\ - --force \\ - $args \\ - $config \\ - $extra_config \\ - $logo \\ - . - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" ) - END_VERSIONS - """ - - stub: - """ - touch multiqc_data - touch multiqc_plots - touch multiqc_report.html - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" ) - END_VERSIONS - """ -} diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml deleted file mode 100644 index f1aa660e..00000000 --- a/modules/nf-core/multiqc/meta.yml +++ /dev/null @@ -1,59 +0,0 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: multiqc -description: Aggregate results from bioinformatics analyses across many samples into a single report -keywords: - - QC - - bioinformatics tools - - Beautiful stand-alone HTML report -tools: - - multiqc: - description: | - MultiQC searches a given directory for analysis logs and compiles a HTML report. - It's a general use tool, perfect for summarising the output from numerous bioinformatics tools. - homepage: https://multiqc.info/ - documentation: https://multiqc.info/docs/ - licence: ["GPL-3.0-or-later"] -input: - - multiqc_files: - type: file - description: | - List of reports / files recognised by MultiQC, for example the html and zip output of FastQC - - multiqc_config: - type: file - description: Optional config yml for MultiQC - pattern: "*.{yml,yaml}" - - extra_multiqc_config: - type: file - description: Second optional config yml for MultiQC. Will override common sections in multiqc_config. - pattern: "*.{yml,yaml}" - - multiqc_logo: - type: file - description: Optional logo file for MultiQC - pattern: "*.{png}" -output: - - report: - type: file - description: MultiQC report file - pattern: "multiqc_report.html" - - data: - type: directory - description: MultiQC data dir - pattern: "multiqc_data" - - plots: - type: file - description: Plots created by MultiQC - pattern: "*_data" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@abhi18av" - - "@bunop" - - "@drpatelh" - - "@jfy133" -maintainers: - - "@abhi18av" - - "@bunop" - - "@drpatelh" - - "@jfy133" diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test deleted file mode 100644 index c2dad217..00000000 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ /dev/null @@ -1,63 +0,0 @@ -nextflow_process { - - name "Test Process MULTIQC" - script "../main.nf" - process "MULTIQC" - tag "modules" - tag "modules_nfcore" - tag "multiqc" - - test("MULTIQC: FASTQC") { - - when { - params { - outdir = "$outputDir" - } - process { - """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) - input[1] = [] - input[2] = [] - input[3] = [] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert path(process.out.report.get(0)).exists() }, - { assert path(process.out.data.get(0)).exists() }, - { assert path(process.out.versions.get(0)).getText().contains("multiqc") } - ) - } - - } - - test("MULTIQC: FASTQC and a config file") { - - when { - params { - outdir = "$outputDir" - } - process { - """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) - input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) - input[2] = [] - input[3] = [] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert path(process.out.report.get(0)).exists() }, - { assert path(process.out.data.get(0)).exists() }, - { assert path(process.out.versions.get(0)).getText().contains("multiqc") } - ) - } - - } -} diff --git a/modules/nf-core/multiqc/tests/tags.yml b/modules/nf-core/multiqc/tests/tags.yml deleted file mode 100644 index bea6c0d3..00000000 --- a/modules/nf-core/multiqc/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -multiqc: - - modules/nf-core/multiqc/** diff --git a/nextflow.config b/nextflow.config index 3db97ece..92020099 100644 --- a/nextflow.config +++ b/nextflow.config @@ -9,54 +9,87 @@ // Global default params, used in configs params { - // TODO nf-core: Specify your pipeline's command line flags // Input options - input = null - // References - genome = null - igenomes_base = 's3://ngi-igenomes/igenomes/' - igenomes_ignore = false + input = null + // Assemblathon stats options + assemblathon_stats_n_limit = 100 - // MultiQC options - multiqc_config = null - multiqc_title = null - multiqc_logo = null - max_multiqc_email_size = '25.MB' - multiqc_methods_description = null + // NCBI FCS options + ncbi_fcs_adaptor_skip = true + ncbi_fcs_adaptor_empire = 'euk' - // Boilerplate options - outdir = null - publish_dir_mode = 'copy' - email = null - email_on_fail = null - plaintext_email = false - monochrome_logs = false - hook_url = null - help = false - version = false + ncbi_fcs_gx_skip = true + ncbi_fcs_gx_tax_id = null + ncbi_fcs_gx_db_path = null - // Config options - config_profile_name = null - config_profile_description = null - custom_config_version = 'master' - custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" - config_profile_contact = null - config_profile_url = null + // BUSCO options + busco_skip = true + busco_mode = 'geno' + busco_lineage_datasets = null + busco_download_path = null + + // TIDK options + tidk_skip = true + tidk_repeat_seq = 'TTAGGG' + tidk_filter_by_size = false + tidk_filter_size_bp = 1000000 + + // LAI options + lai_skip = true + + // kraken2 options + kraken2_skip = true + kraken2_db_path = null + + // HiC options + hic_skip = true + hic_skip_fastp = false + hic_skip_fastqc = false + hic_fastp_ext_args = '--qualified_quality_phred 20 --length_required 50' + // Synteny options + synteny_skip = true + synteny_between_input_assemblies = true + synteny_xref_assemblies = null + synteny_many_to_many_align = false + synteny_max_gap = 1000000 + synteny_min_bundle_size = 1000 + synteny_plot_1_vs_all = true + synteny_color_by_contig = true + + // Output options + outdir = './results' + email = null // Max resource options - // Defaults only, expecting to be overwritten - max_memory = '128.GB' - max_cpus = 16 - max_time = '240.h' + max_memory = '512.GB' + max_cpus = 16 + max_time = '7.day' + + // Boilerplate options + publish_dir_mode = 'copy' + email_on_fail = null + plaintext_email = false + monochrome_logs = false + hook_url = null + help = false + version = false + + // Config options + config_profile_name = null + config_profile_description = null + custom_config_version = 'master' + custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" + config_profile_contact = null + config_profile_url = null // Schema validation default options - validationFailUnrecognisedParams = false - validationLenientMode = false - validationSchemaIgnoreParams = 'genomes,igenomes_base' - validationShowHiddenParams = false - validate_params = true + validationFailUnrecognisedParams = false + validationLenientMode = false + validationSchemaIgnoreParams = '' + validationShowHiddenParams = false + validate_params = true } @@ -186,12 +219,6 @@ plugins { id 'nf-validation@1.1.3' // Validation of pipeline parameters and creation of an input channel from a sample sheet } -// Load igenomes.config if required -if (!params.igenomes_ignore) { - includeConfig 'conf/igenomes.config' -} else { - params.genomes = [:] -} // Export these variables to prevent local Python/R libraries from conflicting with those in the container // The JULIA depot path has been adjusted to a fixed path `/usr/local/share/julia` that needs to be used for packages in the container. // See https://apeltzer.github.io/post/03-julia-lang-nextflow/ for details on that. Once we have a common agreement on where to keep Julia packages, this is adjustable. @@ -233,9 +260,9 @@ manifest { homePage = 'https://github.com/plant-food-research-open/assemblyqc' description = """AssemblyQC is a NextFlow pipeline which evaluates assembly quality with well-established tools and presents the results in a unified html report.""" mainScript = 'main.nf' - nextflowVersion = '!>=23.04.0' + nextflowVersion = '!>=22.04.3' version = '1.4dev' - doi = '' + doi = '10.5281/zenodo.10647870' } // Load modules.config for DSL2 module specific options diff --git a/nextflow_schema.json b/nextflow_schema.json index 1a13c92b..fd27902b 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -8,25 +8,24 @@ "input_output_options": { "title": "Input/output options", "type": "object", - "fa_icon": "fas fa-terminal", - "description": "Define where the pipeline should find input data and save output data.", - "required": ["input", "outdir"], + "description": "", + "default": "", "properties": { "input": { "type": "string", "format": "file-path", - "exists": true, - "mimetype": "text/csv", - "pattern": "^\\S+\\.csv$", - "description": "Path to comma-separated file containing information about the samples in the experiment.", - "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row.", + "mimetype": "csv", + "schema": "assets/schema_input.json", + "help_text": "FASTA and other associated files for input assemblies provided as a formatted CSV file", + "description": "Input assembly sheet in CSV format", "fa_icon": "fas fa-file-csv" }, "outdir": { "type": "string", "format": "directory-path", "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", - "fa_icon": "fas fa-folder-open" + "fa_icon": "fas fa-folder-open", + "default": "./results" }, "email": { "type": "string", @@ -34,42 +33,258 @@ "fa_icon": "fas fa-envelope", "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" + } + }, + "required": ["input", "outdir"] + }, + "general_stats_options": { + "title": "General stats options", + "type": "object", + "description": "", + "default": "", + "properties": { + "assemblathon_stats_n_limit": { + "type": "integer", + "default": 100, + "help_text": "This number is used to split the scaffolds into contigs to compute contig-related stats such as the number of contigs, N50, etc. NCBI recommendation is 100.", + "description": "The number of 'N's for the unknown gap size" + } + } + }, + "ncbi_fcs_options": { + "title": "NCBI FCS options", + "type": "object", + "description": "", + "default": "", + "properties": { + "ncbi_fcs_adaptor_skip": { + "type": "boolean", + "description": "Skip NCBI FCS Adaptor checking", + "default": true }, - "multiqc_title": { + "ncbi_fcs_adaptor_empire": { "type": "string", - "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", - "fa_icon": "fas fa-file-signature" + "default": "euk", + "enum": ["euk", "prok"], + "description": "Empire for NCBI FCS Adaptor checking" + }, + "ncbi_fcs_gx_skip": { + "type": "boolean", + "description": "Skip NCBI FCS external organism contamination checking", + "default": true + }, + "ncbi_fcs_gx_tax_id": { + "type": "number", + "help_text": "Get correct tax ID from https://www.ncbi.nlm.nih.gov/taxonomy", + "description": "Tax ID for NCBI FCS GX" + }, + "ncbi_fcs_gx_db_path": { + "type": "string", + "format": "directory-path", + "help_text": "NCBI FCS GX DB path\n\nDue to enormity of the DB size, the pipeline does NOT download the data. It must be setup by the user manually before running the pipeline. See instructions for DB setup: https://github.com/ncbi/fcs/wiki/FCS-GX", + "description": "Path to NCBI FCS GX database" } } }, - "reference_genome_options": { - "title": "Reference genome options", + "busco_options": { + "title": "BUSCO options", "type": "object", - "fa_icon": "fas fa-dna", - "description": "Reference genome related files and options required for the workflow.", + "description": "", + "default": "", "properties": { - "genome": { + "busco_skip": { + "type": "boolean", + "description": "Skip BUSCO", + "default": true + }, + "busco_mode": { + "type": "string", + "default": "geno", + "enum": ["geno", "tran", "prot", "genome", "transcriptome", "proteins"], + "help_text": "'geno' or 'genome' for genome assemblies (DNA), 'tran' or 'transcriptome' for transcriptome assemblies (DNA), 'prot' or 'proteins' for annotated gene sets (protein)", + "description": "BUSCO mode" + }, + "busco_lineage_datasets": { "type": "string", - "description": "Name of iGenomes reference.", - "fa_icon": "fas fa-book", - "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details." + "help_text": "Each input assembly is assessed against each lineage. It should be provided as a space-separated list of lineages: 'fungi_odb10 microsporidia_odb10' ", + "pattern": "^(\\w+_odb10\\s)+\\w+_odb10$", + "description": "BUSCO lineages" }, - "fasta": { + "busco_download_path": { "type": "string", + "help_text": "BUSCO DB download path\n\nThe pipeline automatically downloads the required DB if needed", + "description": "Download path for BUSCO", + "format": "directory-path" + } + } + }, + "tidk_options": { + "title": "TIDK options", + "type": "object", + "description": "", + "default": "", + "properties": { + "tidk_skip": { + "type": "boolean", + "description": "Skip telomere identification", + "default": true + }, + "tidk_repeat_seq": { + "type": "string", + "default": "TTAGGG", + "description": "Telomere repeat sequence", + "pattern": "^[ACGT]+$" + }, + "tidk_filter_by_size": { + "type": "boolean", + "description": "Filter size in base-pairs" + }, + "tidk_filter_size_bp": { + "type": "integer", + "default": 1000000, + "description": "Filter size in base-pairs" + } + } + }, + "lai_options": { + "title": "LAI options", + "type": "object", + "description": "", + "default": "", + "properties": { + "lai_skip": { + "type": "boolean", + "default": true, + "description": "Skip LAI estimation" + } + } + }, + "kraken2_options": { + "title": "Kraken2 options", + "type": "object", + "description": "", + "default": "", + "properties": { + "kraken2_skip": { + "type": "boolean", + "default": true, + "description": "Skip Kraken2" + }, + "kraken2_db_path": { + "type": "string", + "description": "Kraken2 database path", + "format": "path" + } + } + }, + "hic_options": { + "title": "HiC options", + "type": "object", + "description": "", + "default": "", + "properties": { + "hic_skip": { + "type": "boolean", + "default": true, + "description": "Skip HiC contact map construction" + }, + "hic_skip_fastp": { + "type": "boolean", + "description": "Skip HiC read trimming" + }, + "hic_skip_fastqc": { + "type": "boolean", + "description": "Skip HiC read QC" + }, + "hic_fastp_ext_args": { + "type": "string", + "default": "--qualified_quality_phred 20 --length_required 50", + "description": "Additional parameters for fastp trimming" + } + } + }, + "synteny_options": { + "title": "Synteny options", + "type": "object", + "description": "", + "default": "", + "properties": { + "synteny_skip": { + "type": "boolean", + "default": true, + "description": "Skip synteny analysis" + }, + "synteny_between_input_assemblies": { + "type": "boolean", + "description": "Create syntenic plots between each pair of input assemblies", + "default": true + }, + "synteny_xref_assemblies": { + "type": "string", + "description": "Reference assemblies for synteny analysis", + "help_text": "FASTA and synteny label tsv files should be provided in a formatted CSV file ", "format": "file-path", - "exists": true, - "mimetype": "text/plain", - "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$", - "description": "Path to FASTA genome file.", - "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", - "fa_icon": "far fa-file-code" - }, - "igenomes_ignore": { + "mimetype": "csv", + "schema": "assets/schema_xref_assemblies.json" + }, + "synteny_many_to_many_align": { + "type": "boolean", + "description": "Include alignment blocks with many-to-many mappings (dnadiff .mcoords file)" + }, + "synteny_max_gap": { + "type": "integer", + "default": 1000000, + "description": "Alignments within this distance are bundled together" + }, + "synteny_min_bundle_size": { + "type": "integer", + "default": 1000, + "description": "After bundling, any bundle smaller than this size is filtered out" + }, + "synteny_plot_1_vs_all": { + "type": "boolean", + "default": true, + "description": "Create a separate synteny plot for each contig of the target assembly versus all contigs of the reference assembly" + }, + "synteny_color_by_contig": { "type": "boolean", - "description": "Do not load the iGenomes reference config.", - "fa_icon": "fas fa-ban", + "default": true, + "description": "Synteny plot is colored by contig" + } + } + }, + "max_job_request_options": { + "title": "Max job request options", + "type": "object", + "fa_icon": "fab fa-acquisitions-incorporated", + "description": "Set the top limit for requested resources for any single job.", + "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", + "properties": { + "max_cpus": { + "type": "integer", + "description": "Maximum number of CPUs that can be requested for any single job.", + "default": 16, + "fa_icon": "fas fa-microchip", "hidden": true, - "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." + "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" + }, + "max_memory": { + "type": "string", + "description": "Maximum amount of memory that can be requested for any single job.", + "default": "512.GB", + "fa_icon": "fas fa-memory", + "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", + "hidden": true, + "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" + }, + "max_time": { + "type": "string", + "description": "Maximum amount of time that can be requested for any single job.", + "default": "7.day", + "fa_icon": "far fa-clock", + "pattern": "^(\\d+\\.?\\s*(s|m|h|d|day)\\s*)+$", + "hidden": true, + "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" } } }, @@ -121,41 +336,6 @@ } } }, - "max_job_request_options": { - "title": "Max job request options", - "type": "object", - "fa_icon": "fab fa-acquisitions-incorporated", - "description": "Set the top limit for requested resources for any single job.", - "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", - "properties": { - "max_cpus": { - "type": "integer", - "description": "Maximum number of CPUs that can be requested for any single job.", - "default": 16, - "fa_icon": "fas fa-microchip", - "hidden": true, - "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" - }, - "max_memory": { - "type": "string", - "description": "Maximum amount of memory that can be requested for any single job.", - "default": "128.GB", - "fa_icon": "fas fa-memory", - "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", - "hidden": true, - "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" - }, - "max_time": { - "type": "string", - "description": "Maximum amount of time that can be requested for any single job.", - "default": "240.h", - "fa_icon": "far fa-clock", - "pattern": "^(\\d+\\.?\\s*(s|m|h|d|day)\\s*)+$", - "hidden": true, - "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" - } - } - }, "generic_options": { "title": "Generic options", "type": "object", @@ -198,14 +378,6 @@ "fa_icon": "fas fa-remove-format", "hidden": true }, - "max_multiqc_email_size": { - "type": "string", - "description": "File size limit when attaching MultiQC reports to summary emails.", - "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", - "default": "25.MB", - "fa_icon": "fas fa-file-upload", - "hidden": true - }, "monochrome_logs": { "type": "boolean", "description": "Do not use coloured log outputs.", @@ -219,24 +391,6 @@ "help_text": "Incoming hook URL for messaging service. Currently, MS Teams and Slack are supported.", "hidden": true }, - "multiqc_config": { - "type": "string", - "format": "file-path", - "description": "Custom config file to supply to MultiQC.", - "fa_icon": "fas fa-cog", - "hidden": true - }, - "multiqc_logo": { - "type": "string", - "description": "Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file", - "fa_icon": "fas fa-image", - "hidden": true - }, - "multiqc_methods_description": { - "type": "string", - "description": "Custom MultiQC yaml file containing HTML including a methods description.", - "fa_icon": "fas fa-cog" - }, "validate_params": { "type": "boolean", "description": "Boolean whether to validate parameters against the schema at runtime", @@ -273,14 +427,35 @@ "$ref": "#/definitions/input_output_options" }, { - "$ref": "#/definitions/reference_genome_options" + "$ref": "#/definitions/general_stats_options" }, { - "$ref": "#/definitions/institutional_config_options" + "$ref": "#/definitions/ncbi_fcs_options" + }, + { + "$ref": "#/definitions/busco_options" + }, + { + "$ref": "#/definitions/tidk_options" + }, + { + "$ref": "#/definitions/lai_options" + }, + { + "$ref": "#/definitions/kraken2_options" + }, + { + "$ref": "#/definitions/hic_options" + }, + { + "$ref": "#/definitions/synteny_options" }, { "$ref": "#/definitions/max_job_request_options" }, + { + "$ref": "#/definitions/institutional_config_options" + }, { "$ref": "#/definitions/generic_options" } diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf deleted file mode 100644 index 0aecf87f..00000000 --- a/subworkflows/local/input_check.nf +++ /dev/null @@ -1,44 +0,0 @@ -// -// Check input samplesheet and get read channels -// - -include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' - -workflow INPUT_CHECK { - take: - samplesheet // file: /path/to/samplesheet.csv - - main: - SAMPLESHEET_CHECK ( samplesheet ) - .csv - .splitCsv ( header:true, sep:',' ) - .map { create_fastq_channel(it) } - .set { reads } - - emit: - reads // channel: [ val(meta), [ reads ] ] - versions = SAMPLESHEET_CHECK.out.versions // channel: [ versions.yml ] -} - -// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] -def create_fastq_channel(LinkedHashMap row) { - // create meta map - def meta = [:] - meta.id = row.sample - meta.single_end = row.single_end.toBoolean() - - // add path(s) of the fastq file(s) to the meta map - def fastq_meta = [] - if (!file(row.fastq_1).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" - } - if (meta.single_end) { - fastq_meta = [ meta, [ file(row.fastq_1) ] ] - } else { - if (!file(row.fastq_2).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" - } - fastq_meta = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] - } - return fastq_meta -} diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index db0a1dd5..56f21437 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -4,7 +4,7 @@ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryLog; paramsSummaryMap; fromSamplesheet } from 'plugin/nf-validation' def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) def citation = '\n' + WorkflowMain.citation(workflow) + '\n' @@ -21,10 +21,7 @@ WorkflowAssemblyqc.initialise(params, log) ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath( params.multiqc_config, checkIfExists: true ) : Channel.empty() -ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath( params.multiqc_logo, checkIfExists: true ) : Channel.empty() -ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) +// ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -35,7 +32,7 @@ ch_multiqc_custom_methods_description = params.multiqc_methods_description ? fil // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules // -include { INPUT_CHECK } from '../subworkflows/local/input_check' +// include { INPUT_CHECK } from '../subworkflows/local/input_check' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -46,9 +43,13 @@ include { INPUT_CHECK } from '../subworkflows/local/input_check' // // MODULE: Installed directly from nf-core/modules // -include { FASTQC } from '../modules/nf-core/fastqc/main' -include { MULTIQC } from '../modules/nf-core/multiqc/main' -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' + +include { GUNZIP as GUNZIP_FASTA } from '../modules/nf-core/gunzip/main' +include { GUNZIP as GUNZIP_GFF3 } from '../modules/nf-core/gunzip/main' +include { FASTAVALIDATOR } from '../modules/nf-core/fastavalidator/main' + +include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -57,57 +58,65 @@ include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoft */ // Info required for completion email and summary -def multiqc_report = [] +def assemblyqc_report = [] workflow ASSEMBLYQC { - ch_versions = Channel.empty() + ch_versions = Channel.empty() + ch_input = Channel.fromSamplesheet('input') + + ch_target_assemby_branch = ch_input + | map { tag, fasta, gff, ids, reads, labels -> + [ [ id: tag ], file(fasta, checkIfExists: true) ] + } + | branch { meta, fasta -> + gz: "$fasta".endsWith(".gz") + rest: !"$fasta".endsWith(".gz") + } + + ch_assemby_gff3_branch = ch_input + | map { tag, fasta, gff, ids, reads, labels -> + gff + ? [ [ id: tag ], file(gff, checkIfExists: true) ] + : null + } + | branch { meta, gff -> + gz: "$gff".endsWith(".gz") + rest: !"$gff".endsWith(".gz") + } + + // MODULE: GUNZIP as GUNZIP_FASTA + GUNZIP_FASTA ( ch_target_assemby_branch.gz ) + + ch_target_assembly = GUNZIP_FASTA.out.gunzip.mix(ch_target_assemby_branch.rest) + ch_versions = ch_versions.mix(GUNZIP_FASTA.out.versions.first()) - // - // SUBWORKFLOW: Read in samplesheet, validate and stage input files - // - INPUT_CHECK ( - file(params.input) - ) - ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) - // TODO: OPTIONAL, you can use nf-validation plugin to create an input channel from the samplesheet with Channel.fromSamplesheet("input") - // See the documentation https://nextflow-io.github.io/nf-validation/samplesheets/fromSamplesheet/ - // ! There is currently no tooling to help you write a sample sheet schema - - // - // MODULE: Run FastQC - // - FASTQC ( - INPUT_CHECK.out.reads - ) - ch_versions = ch_versions.mix(FASTQC.out.versions.first()) + // MODULE: GUNZIP as GUNZIP_GFF3 + GUNZIP_GFF3 ( ch_assemby_gff3_branch.gz ) + + ch_assembly_gff3 = GUNZIP_GFF3.out.gunzip.mix(ch_assemby_gff3_branch.rest) + ch_versions = ch_versions.mix(GUNZIP_GFF3.out.versions.first()) + + // MODULE: FASTAVALIDATOR + FASTAVALIDATOR ( ch_target_assembly ) + + ch_valid_target_assembly = ch_target_assembly.join(FASTAVALIDATOR.out.success_log) + | map { meta, fasta, log -> [ meta, fasta ] } + + ch_invalid_assembly_log = FASTAVALIDATOR.out.error_log + | map { meta, error_log -> + log.warn("FASTA validation failed for ${meta.id}\n${error_log.text}") + + [ meta, error_log ] + } + + ch_versions = ch_versions.mix(FASTAVALIDATOR.out.versions.first()) + + // MODULE: CUSTOM_DUMPSOFTWAREVERSIONS CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') ) - - // - // MODULE: MultiQC - // - workflow_summary = WorkflowAssemblyqc.paramsSummaryMultiqc(workflow, summary_params) - ch_workflow_summary = Channel.value(workflow_summary) - - methods_description = WorkflowAssemblyqc.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) - ch_methods_description = Channel.value(methods_description) - - ch_multiqc_files = Channel.empty() - ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) - - MULTIQC ( - ch_multiqc_files.collect(), - ch_multiqc_config.toList(), - ch_multiqc_custom_config.toList(), - ch_multiqc_logo.toList() - ) - multiqc_report = MULTIQC.out.report.toList() } /* @@ -118,7 +127,7 @@ workflow ASSEMBLYQC { workflow.onComplete { if (params.email || params.email_on_fail) { - NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) + NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, assemblyqc_report) } NfcoreTemplate.dump_parameters(workflow, params) NfcoreTemplate.summary(workflow, params, log) From d4cc276132b1077462d2612ce5315225036da210 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 20 Feb 2024 16:05:41 +1300 Subject: [PATCH 09/65] Updated README and removed release_assnouncments action --- .editorconfig | 2 +- .github/workflows/release-announcements.yml | 68 ------------- CITATION.cff | 27 ++++++ CITATIONS.md | 102 +++++++++++++++++++- README.md | 100 ++++++++++++------- conf/base.config | 1 - nextflow.config | 2 +- 7 files changed, 193 insertions(+), 109 deletions(-) delete mode 100644 .github/workflows/release-announcements.yml create mode 100644 CITATION.cff diff --git a/.editorconfig b/.editorconfig index 9b990088..63eff3a4 100644 --- a/.editorconfig +++ b/.editorconfig @@ -8,7 +8,7 @@ trim_trailing_whitespace = true indent_size = 4 indent_style = space -[*.{md,yml,yaml,html,css,scss,js}] +[*.{md,yml,yaml,html,css,scss,js,cff}] indent_size = 2 # These files are edited and tested upstream in nf-core/modules diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml deleted file mode 100644 index 21ac3f06..00000000 --- a/.github/workflows/release-announcements.yml +++ /dev/null @@ -1,68 +0,0 @@ -name: release-announcements -# Automatic release toot and tweet anouncements -on: - release: - types: [published] - workflow_dispatch: - -jobs: - toot: - runs-on: ubuntu-latest - steps: - - uses: rzr/fediverse-action@master - with: - access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }} - host: "mstdn.science" # custom host if not "mastodon.social" (default) - # GitHub event payload - # https://docs.github.com/en/developers/webhooks-and-events/webhooks/webhook-events-and-payloads#release - message: | - Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! - - Please see the changelog: ${{ github.event.release.html_url }} - - send-tweet: - runs-on: ubuntu-latest - - steps: - - uses: actions/setup-python@v5 - with: - python-version: "3.10" - - name: Install dependencies - run: pip install tweepy==4.14.0 - - name: Send tweet - shell: python - run: | - import os - import tweepy - - client = tweepy.Client( - access_token=os.getenv("TWITTER_ACCESS_TOKEN"), - access_token_secret=os.getenv("TWITTER_ACCESS_TOKEN_SECRET"), - consumer_key=os.getenv("TWITTER_CONSUMER_KEY"), - consumer_secret=os.getenv("TWITTER_CONSUMER_SECRET"), - ) - tweet = os.getenv("TWEET") - client.create_tweet(text=tweet) - env: - TWEET: | - Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! - - Please see the changelog: ${{ github.event.release.html_url }} - TWITTER_CONSUMER_KEY: ${{ secrets.TWITTER_CONSUMER_KEY }} - TWITTER_CONSUMER_SECRET: ${{ secrets.TWITTER_CONSUMER_SECRET }} - TWITTER_ACCESS_TOKEN: ${{ secrets.TWITTER_ACCESS_TOKEN }} - TWITTER_ACCESS_TOKEN_SECRET: ${{ secrets.TWITTER_ACCESS_TOKEN_SECRET }} - - bsky-post: - runs-on: ubuntu-latest - steps: - - uses: zentered/bluesky-post-action@v0.1.0 - with: - post: | - Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! - - Please see the changelog: ${{ github.event.release.html_url }} - env: - BSKY_IDENTIFIER: ${{ secrets.BSKY_IDENTIFIER }} - BSKY_PASSWORD: ${{ secrets.BSKY_PASSWORD }} - # diff --git a/CITATION.cff b/CITATION.cff new file mode 100644 index 00000000..efb1b209 --- /dev/null +++ b/CITATION.cff @@ -0,0 +1,27 @@ +cff-version: 1.2.0 +message: "If you use this pipeline, please cite it as below." +authors: + - family-names: "Rashid" + given-names: "Usman" + orcid: "https://orcid.org/0000-0002-1109-5493" + - family-names: "Wu" + given-names: "Chen" + - family-names: "Shiller" + given-names: "Jason" + - family-names: "Smith" + given-names: "Ken" + - family-names: "Crowhurst" + given-names: "Ross" + - family-names: "Davy" + given-names: "Marcus" + - family-names: "Chen" + given-names: "Ting-Hsuan" + - family-names: "Thomson" + given-names: "Susan" + - family-names: "Deng" + given-names: "Cecilia" +title: "AssemblyQC: A NextFlow pipeline for evaluating assembly quality" +version: 1.4 +date-released: 2024-02-12 +url: "https://github.com/Plant-Food-Research-Open/assembly_qc" +doi: 10.5281/zenodo.10647870 diff --git a/CITATIONS.md b/CITATIONS.md index 2b468811..63cbdd2b 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -10,13 +10,107 @@ ## Pipeline tools -- [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) +- py_fasta_validator, [MIT](https://github.com/linsalrob/py_fasta_validator/blob/master/LICENSE) - > Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. + > Edwards, R.A. 2019. fasta_validate: a fast and efficient fasta validator written in pure C. doi: -- [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) +- GenomeTools, [ISC](http://genometools.org/license.html) - > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. + > Gremme G, Steinbiss S, Kurtz S. 2013. "GenomeTools: A Comprehensive Software Library for Efficient Processing of Structured Genome Annotations," in IEEE/ACM Transactions on Computational Biology and Bioinformatics, vol. 10, no. 3, pp. 645-656, May 2013, doi: + +- SAMTOOLS, [MIT/Expat](https://github.com/samtools/samtools/blob/develop/LICENSE) + + > Danecek P, Bonfield JK, Liddle J, Marshall J, Ohan V, Pollard MO, Whitwham A, Keane T, McCarthy SA, Davies RM, Li H. 2021. Twelve years of SAMtools and BCFtools, GigaScience, Volume 10, Issue 2, February 2021, giab008, + +- NCBI/FCS, [License](https://github.com/ncbi/fcs/blob/main/LICENSE.txt) + + > Astashyn A, Tvedte ES, Sweeney D, Sapojnikov V, Bouk N, Joukov V, Mozes E, Strope PK, Sylla PM, Wagner L, Bidwell SL, Clark K, Davis EW, Smith-White B, Hlavina W, Pruitt KD, Schneider VA, Murphy TD. 2023. Rapid and sensitive detection of genome contamination at scale with FCS-GX. bioRxiv 2023.06.02.543519; doi: + +- KRONA, [License](https://github.com/marbl/Krona/blob/master/KronaTools/LICENSE.txt) + + > Ondov BD, Bergman NH, Phillippy AM. 2011. Interactive metagenomic visualization in a Web browser. BMC Bioinformatics. 2011 Sep 30;12:385. doi: + +- assemblathon_stats, [CC BY-NC-SA 3.0](https://creativecommons.org/licenses/by-nc-sa/3.0/) + + > [github/PlantandFoodResearch/assemblathon2-analysis/a93cba2](https://github.com/PlantandFoodResearch/assemblathon2-analysis/blob/a93cba25d847434f7eadc04e63b58c567c46a56d/assemblathon_stats.pl) + > + > Forked from: + +- BUSCO, [MIT](https://gitlab.com/ezlab/busco/-/blob/master/LICENSE) + + > Manni M, Berkeley MR, Seppey M, Simão FA, Zdobnov EM. 2021. BUSCO Update: Novel and Streamlined Workflows along with Broader and Deeper Phylogenetic Coverage for Scoring of Eukaryotic, Prokaryotic, and Viral Genomes, Molecular Biology and Evolution, Volume 38, Issue 10, October 2021, Pages 4647–4654, + +- TIDK, [MIT](https://github.com/tolkit/telomeric-identifier/blob/main/LICENSE) + + > + +- SEQKIT, [MIT](https://github.com/shenwei356/seqkit/blob/master/LICENSE) + + > Shen W, Le S, Li Y, Hu F. 2016. SeqKit: A Cross-Platform and Ultrafast Toolkit for FASTA/Q File Manipulation. PLoS ONE 11(10): e0163962. + +- LAI, [GPL v3](https://github.com/oushujun/LTR_retriever/blob/master/LICENSE) + + > Ou S, Chen J, Jiang N. 2018. Assessing genome assembly quality using the LTR Assembly Index (LAI), Nucleic Acids Research, Volume 46, Issue 21, 30 November 2018, Page e126, + +- LTR_FINDER_parallel, [MIT](https://github.com/oushujun/LTR_FINDER_parallel/blob/master/LICENSE) + + > Ou S, Jiang N 2019. LTR_FINDER_parallel: parallelization of LTR_FINDER enabling rapid identification of long terminal repeat retrotransposons. Mobile DNA 10, 48 (2019). + +- LTRharvest, [ISC](http://genometools.org/license.html) + + > Ellinghaus, D, Kurtz, S & Willhoeft, U 2008. LTRharvest, an efficient and flexible software for de novo detection of LTR retrotransposons. BMC Bioinformatics 9, 18 (2008). + +- LTR_retriever, [GPL v3](https://github.com/oushujun/LTR_retriever/blob/master/LICENSE) + + > Shujun O, Ning J 2018. LTR_retriever: A Highly Accurate and Sensitive Program for Identification of Long Terminal Repeat Retrotransposons, Plant Physiology, 176, 2 (2018). + +- KRAKEN2, [MIT](https://github.com/DerrickWood/kraken2/blob/master/LICENSE) + + > Wood DE, Salzberg SL, Wood DE, Lu J, Langmead B. 2019. Improved metagenomic analysis with Kraken 2. Genome Biol 20, 257 (2019). + +- JUICEBOX.JS, [MIT](https://github.com/igvteam/juicebox.js/blob/master/LICENSE) + + > Robinson JT, Turner D, Durand NC, Thorvaldsdóttir H, Mesirov JP, Aiden EL. 2018. Juicebox.js Provides a Cloud-Based Visualization System for Hi-C Data. Cell Syst. 2018 Feb 28;6(2):256-258.e1. doi: . Epub 2018 Feb 7. PMID: 29428417; PMCID: PMC6047755. + +- FASTP, [MIT](https://github.com/OpenGene/fastp/blob/master/LICENSE) + + > Chen S, Zhou Y, Chen Y, Gu J. 2018. fastp: an ultra-fast all-in-one FASTQ preprocessor, Bioinformatics, Volume 34, Issue 17, 01 September 2018, Pages i884–i890, + +- FASTQC, [GPL v3](https://github.com/s-andrews/FastQC/blob/master/LICENSE.txt) + + > + +- run-assembly-visualizer.sh, [MIT](https://github.com/aidenlab/3d-dna/blob/master/LICENSE) + + > Dudchenko O, Batra SS, Omer AD, Nyquist SK, Hoeger M, Durand NC, Shamim MS, Machol I, Lander, Aiden AP, Aiden EL 2017. De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds.Science356, 92-95(2017). doi: . Available at: + +- HIC_QC, [AGPL v3](https://github.com/phasegenomics/hic_qc/blob/master/LICENSE) + + > + +- JUICEBOX_SCRIPTS, [AGPL v3](https://github.com/phasegenomics/juicebox_scripts/blob/master/LICENSE) + + > + +- BWA, [GPL v3](https://github.com/lh3/bwa/blob/master/COPYING) + + > Li H. 2013. Aligning sequence reads, clone sequences and assembly contigs with BWA-MEM. + +- MATLOCK, [AGPL v3](https://github.com/phasegenomics/matlock/blob/master/LICENSE) + + > ; + +- SAMBLASTER, [MIT](https://github.com/GregoryFaust/samblaster/blob/master/LICENSE.txt) + + > Faust GG, Hall IM. 2014. SAMBLASTER: fast duplicate marking and structural variant read extraction, Bioinformatics, Volume 30, Issue 17, September 2014, Pages 2503–2505, + +- CIRCOS, [GPL v3](https://www.gnu.org/licenses/gpl-3.0.txt) + + > Krzywinski M, Schein J, Birol I, Connors J, Gascoyne R. Horsman D, ... Marra MA. 2009. Circos: an information aesthetic for comparative genomics. Genome research, 19(9), 1639-1645. + +- MUMMER, [Artistic 2.0](https://github.com/mummer4/mummer/blob/master/LICENSE.md) + + > Marçais G, Delcher AL, Phillippy AM, Coston R, Salzberg SL, Zimin A. 2018. MUMmer4: A fast and versatile genome alignment system. PLoS Comput Biol. 2018 Jan 26;14(1):e1005944. doi: . PMID: 29373581; PMCID: PMC5802927. ## Software packaging/containerisation tools diff --git a/README.md b/README.md index 85f09fe3..da8711ce 100644 --- a/README.md +++ b/README.md @@ -1,5 +1,5 @@ [![GitHub Actions CI Status](https://github.com/plant-food-research-open/assemblyqc/workflows/nf-core%20CI/badge.svg)](https://github.com/plant-food-research-open/assemblyqc/actions?query=workflow%3A%22nf-core+CI%22) -[![GitHub Actions Linting Status](https://github.com/plant-food-research-open/assemblyqc/workflows/nf-core%20linting/badge.svg)](https://github.com/plant-food-research-open/assemblyqc/actions?query=workflow%3A%22nf-core+linting%22)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) +[![GitHub Actions Linting Status](https://github.com/plant-food-research-open/assemblyqc/workflows/nf-core%20linting/badge.svg)](https://github.com/plant-food-research-open/assemblyqc/actions?query=workflow%3A%22nf-core+linting%22)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.10647870-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.10647870) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A522.04.3-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) @@ -9,50 +9,79 @@ ## Introduction -**plant-food-research-open/assemblyqc** is a bioinformatics pipeline that ... +**plant-food-research-open/assemblyqc** is a [NextFlow](https://www.nextflow.io/docs/latest/index.html) pipeline which evaluates assembly quality with well-established tools and presents the results in a unified html report. The tools are shown in the [Pipeline Flowchart](#pipeline-flowchart) and their version are listed in [CITATIONS.md](./CITATIONS.md). - +## Pipeline Flowchart - - +```mermaid +flowchart LR + forEachTag(For each\nAssembly) --> VALIDATE_FORMAT[VALIDATE FORMAT] -1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) -2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/)) + VALIDATE_FORMAT --> ncbiFCS[NCBI FCS\nADAPTOR] + ncbiFCS --> Check{Check} -## Usage - -> [!NOTE] -> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. + VALIDATE_FORMAT --> ncbiGX[NCBI FCS GX] + ncbiGX --> Check + Check --> |Clean|Run(Run) - |Contamination|Skip(Skip All) + Skip --> REPORT -First, prepare a samplesheet with your input data that looks as follows: + VALIDATE_FORMAT --> GFF_STATS[GENOMETOOLS GT STAT] -`samplesheet.csv`: + Run --> ASS_STATS[ASSEMBLATHON STATS] + Run --> BUSCO + Run --> TIDK + Run --> LTRRETRIEVER + LTRRETRIEVER --> LAI + Run --> KRAKEN2 + Run --> HIC_CONTACT_MAP[HIC CONTACT MAP] + Run --> SYNTENY -```csv -sample,fastq_1,fastq_2 -CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz + ASS_STATS --> REPORT + GFF_STATS --> REPORT + BUSCO --> REPORT + TIDK --> REPORT + LAI --> REPORT + KRAKEN2 --> REPORT + HIC_CONTACT_MAP --> REPORT + SYNTENY --> REPORT ``` -Each row represents a fastq file (single-end) or a pair of fastq files (paired end). +- [FASTA VALIDATION](https://github.com/GallVp/fasta_validator) +- [GFF3 VALIDATION](https://github.com/genometools/genometools) +- [ASSEMBLATHON STATS](https://github.com/PlantandFoodResearch/assemblathon2-analysis/blob/a93cba25d847434f7eadc04e63b58c567c46a56d/assemblathon_stats.pl): Assembly statistics +- [GENOMETOOLS GT STAT](https://github.com/genometools/genometools): Annotation statistics +- [NCBI FCS ADAPTOR](https://github.com/ncbi/fcs): Adaptor contamination pass/fail +- [NCBI FCS GX](https://github.com/ncbi/fcs): Foreign organism contamination pass/fail +- [BUSCO](https://gitlab.com/ezlab/busco/-/tree/master): Gene-space completeness estimation +- [TIDK](https://github.com/tolkit/telomeric-identifier): Telomere repeat identification +- [LAI](https://github.com/oushujun/LTR_retriever/blob/master/LAI): Continuity of repetitive sequences +- [LAI::LTRRETRIEVER](https://github.com/oushujun/LTR_retriever): Repeat identification +- [KRAKEN2](https://github.com/DerrickWood/kraken2): Taxonomy classification +- [HIC CONTACT MAP](https://github.com/igvteam/juicebox-web): Alignment and visualisation of HiC data +- SYNTENY: Synteny analysis using [MUMMER](https://github.com/mummer4/mummer) and [CIRCOS](http://circos.ca/documentation/) ---> +## Usage -Now, you can run the pipeline using: +> [!NOTE] +> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. + +Prepare an `assemblysheet.csv` file with following columns representing target assemblies and associated meta-data. - +- tag: A unique tag which represents the target assembly throughout the pipeline and in the final report +- fasta: FASTA file +- gff3 [Optional]: GFF3 annotation file if available +- monoploid_ids [Optional]: A txt file listing the IDs used to calculate LAI in monoploid mode if necessary +- hic_reads [Optional] A SRA id such as 'SRR8238190' or path to paired reads such as 'PG_PETUNIA_HiC_CGYCF_CACTCA_L001_R{1,2}.fastq.gz' +- synteny_labels [Optional]: A two column tsv file listing fasta sequence ids (first column) and labels for the synteny plots (second column) when performing synteny analysis + +Now, you can run the pipeline using: ```bash nextflow run plant-food-research-open/assemblyqc \ -profile \ - --input samplesheet.csv \ + --input assemblysheet.csv \ --outdir ``` @@ -62,11 +91,17 @@ nextflow run plant-food-research-open/assemblyqc \ ## Credits -plant-food-research-open/assemblyqc was originally written by Usman Rashid. +plant-food-research-open/assemblyqc was originally written by Usman Rashid and Ken Smith. We thank the following people for their extensive assistance in the development of this pipeline: - +- Cecilia Deng [@CeciliaDeng](https://github.com/CeciliaDeng) +- Chen Wu [@christinawu2008](https://github.com/christinawu2008) +- Jason Shiller [@jasonshiller](https://github.com/jasonshiller) +- Marcus Davy [@mdavy86](https://github.com/mdavy86) +- Ross Crowhurst [@rosscrowhurst](https://github.com/rosscrowhurst) +- Susan Thomson [@cflsjt](https://github.com/cflsjt) +- Ting-Hsuan Chen [@ting-hsuan-chen](https://github.com/ting-hsuan-chen) ## Contributions and Support @@ -74,10 +109,7 @@ If you would like to contribute to this pipeline, please see the [contributing g ## Citations - - - - +If you use plant-food-research-open/assemblyqc for your analysis, please cite it using the following doi: [10.5281/zenodo.10647870](https://doi.org/10.5281/zenodo.10647870) An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file. diff --git a/conf/base.config b/conf/base.config index fad3aa91..cf60eac8 100644 --- a/conf/base.config +++ b/conf/base.config @@ -10,7 +10,6 @@ process { - // TODO nf-core: Check the defaults for all processes cpus = { check_max( 1 * task.attempt, 'cpus' ) } memory = { check_max( 6.GB * task.attempt, 'memory' ) } time = { check_max( 4.h * task.attempt, 'time' ) } diff --git a/nextflow.config b/nextflow.config index 92020099..1af86d10 100644 --- a/nextflow.config +++ b/nextflow.config @@ -258,7 +258,7 @@ manifest { name = 'plant-food-research-open/assemblyqc' author = """Usman Rashid""" homePage = 'https://github.com/plant-food-research-open/assemblyqc' - description = """AssemblyQC is a NextFlow pipeline which evaluates assembly quality with well-established tools and presents the results in a unified html report.""" + description = """A NextFlow pipeline which evaluates assembly quality with well-established tools and presents the results in a unified html report.""" mainScript = 'main.nf' nextflowVersion = '!>=22.04.3' version = '1.4dev' From 51f75d4263b15b5dc7ed27ed879418312eceba43 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 20 Feb 2024 16:06:45 +1300 Subject: [PATCH 10/65] Fixed template issues --- assets/email_template.html | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/assets/email_template.html b/assets/email_template.html index fa1b2da2..be11e5e0 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -4,7 +4,7 @@ - + plant-food-research-open/assemblyqc Pipeline Report From cd0e900007e47908f2b8065c7a9d9f9c96204b3d Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 20 Feb 2024 16:18:19 +1300 Subject: [PATCH 11/65] Cleared additional linting checks --- assets/methods_description_template.yml | 29 ------------------------- assets/multiqc_config.yml | 12 ---------- nextflow_schema.json | 2 +- 3 files changed, 1 insertion(+), 42 deletions(-) delete mode 100644 assets/methods_description_template.yml delete mode 100644 assets/multiqc_config.yml diff --git a/assets/methods_description_template.yml b/assets/methods_description_template.yml deleted file mode 100644 index e2a5645c..00000000 --- a/assets/methods_description_template.yml +++ /dev/null @@ -1,29 +0,0 @@ -id: "plant-food-research-open-assemblyqc-methods-description" -description: "Suggested text and references to use when describing pipeline usage within the methods section of a publication." -section_name: "plant-food-research-open/assemblyqc Methods Description" -section_href: "https://github.com/plant-food-research-open/assemblyqc" -plot_type: "html" -## TODO nf-core: Update the HTML below to your preferred methods description, e.g. add publication citation for this pipeline -## You inject any metadata in the Nextflow '${workflow}' object -data: | -

    Methods

    -

    Data was processed using plant-food-research-open/assemblyqc v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

    -

    The pipeline was executed with Nextflow v${workflow.nextflow.version} (Di Tommaso et al., 2017) with the following command:

    - 
    ${workflow.commandLine}
    -

    ${tool_citations}

    -

    References

    -
      -
    • Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. doi: 10.1038/nbt.3820
      • -
    • Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. doi: 10.1038/s41587-020-0439-x
      • -
    • Grüning, B., Dale, R., Sjödin, A., Chapman, B. A., Rowe, J., Tomkins-Tinch, C. H., Valieris, R., Köster, J., & Bioconda Team. (2018). Bioconda: sustainable and comprehensive software distribution for the life sciences. Nature Methods, 15(7), 475–476. doi: 10.1038/s41592-018-0046-7
      • -
    • da Veiga Leprevost, F., Grüning, B. A., Alves Aflitos, S., Röst, H. L., Uszkoreit, J., Barsnes, H., Vaudel, M., Moreno, P., Gatto, L., Weber, J., Bai, M., Jimenez, R. C., Sachsenberg, T., Pfeuffer, J., Vera Alvarez, R., Griss, J., Nesvizhskii, A. I., & Perez-Riverol, Y. (2017). BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics (Oxford, England), 33(16), 2580–2582. doi: 10.1093/bioinformatics/btx192
      • - ${tool_bibliography} -
    -
    -
    Notes:
    -
      - ${nodoi_text} -
    • The command above does not include parameters contained in any configs or profiles that may have been used. Ensure the config file is also uploaded with your publication!
      • -
    • You should also cite all software used within this run. Check the "Software Versions" of this report to get version information.
      • -
    -
    diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml deleted file mode 100644 index 6d8df9b7..00000000 --- a/assets/multiqc_config.yml +++ /dev/null @@ -1,12 +0,0 @@ -report_comment: > - This report has been generated by the plant-food-research-open/assemblyqc - analysis pipeline. -report_section_order: - "plant-food-research-open-assemblyqc-methods-description": - order: -1000 - software_versions: - order: -1001 - "plant-food-research-open-assemblyqc-summary": - order: -1002 - -export_plots: true diff --git a/nextflow_schema.json b/nextflow_schema.json index fd27902b..94599989 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -2,7 +2,7 @@ "$schema": "http://json-schema.org/draft-07/schema", "$id": "https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/master/nextflow_schema.json", "title": "plant-food-research-open/assemblyqc pipeline parameters", - "description": "AssemblyQC is a NextFlow pipeline which evaluates assembly quality with well-established tools and presents the results in a unified html report.", + "description": "A NextFlow pipeline which evaluates assembly quality with well-established tools and presents the results in a unified html report.", "type": "object", "definitions": { "input_output_options": { From 5c03bab106aafa52aefce348a04880e716cd4b13 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 20 Feb 2024 16:29:20 +1300 Subject: [PATCH 12/65] Added CHANGELOG --- CHANGELOG.md | 292 ++++++++++++++++++++++++++++++++++++++++++++++++++- 1 file changed, 289 insertions(+), 3 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 4aed6c94..530a7213 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,14 +3,300 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v1.4dev - [date] - -Initial release of plant-food-research-open/assemblyqc, created with the [nf-core](https://nf-co.re/) template. +## v1.4dev - [20-Feb-2024] ### `Added` +1. Now it is possible to skip FASTP and FASTQC for the HIC module +2. Renamed ASSEMBLY_QC workflow to ASSEMBLYQC +3. Now using nf-core/FASTA_EXPLORE_SEARCH_PLOT_TIDK +4. Now redirecting validation errors to AssemblyQC report +5. Simplified layout of CITATIONS.md file +6. Now using pfr/gff3_validate sub-workflow for gff3 validation +7. Now listing software versions from the versions.yml file +8. Replaced custom GUNZIP module with nf-core/gunzip +9. Replaced custom gt/stat with pfr/gt/stat +10. Replaced custom fasta_validator with nf-core/fastavalidator +11. Removed BIOCODE GFF3 STATS owing to its frequent failures +12. Added pre-commit version checking +13. Now gt/stat reports extended stats and multiple distribution plots have been added to the report +14. Added a tools tab to the report which lists the tools used by the pipeline to create the report +15. Refactored and cleaned data flows for all the custom sub-workflow +16. Started using nf-core template +17. Started using semantic versioning + ### `Fixed` ### `Dependencies` ### `Deprecated` + +## v1.3 [08-Feb-2023] + +1. Docker engine is now also supported +2. Added Amazon Genomics CLI project file and a minimal test params file: [./docs/test_params/test_agc.json](./docs/test_params/test_agc.json) +3. Downgraded to Nextflow 22.04.3 +4. Removed container setup process from NCBI_FCS_ADAPTOR workflow +5. The pipeline does not download the kraken database anymore +6. Fixed a bug in SYNTENY/DNADIFF module which caused failure on AWS Batch +7. Now tar zipped database can be directly used with Kraken2 +8. Removed `db_manifest_url` parameter for the NCBI_FCS_GX workflow +9. Now using parallel version of LTRHARVEST from the EDTA package +10. BWA_INDEX_AND_MEM can now run for two days +11. Now using FASTQ_BWA_MEM_SAMBLASTER subworkflow to optimize SAM file transfer on AWS + +## v1.2 [18-Dec-2023] + +1. Switched to apptainer from singularity +2. Now requiring Nextflow/23.04.4 +3. Simplified output directory from `outdir.main` to `outdir` +4. Changed profile name from slurm to pfr +5. Now using APPTAINER_BINDPATH to provide TMPDIR +6. Integrated and tested FASTA_LTRRETRIEVER_LAI to replace EDTA_LAI sub-workflow +7. Corrected LAI version to beta3.2 + +### FASTA_LTRRETRIEVER_LAI vs EDTA_LAI + +For a ~600 MB assembly, EDTA (without sensitive flag) takes ~25 hours of compute time. Whereas, FASTA_LTRRETRIEVER_LAI sub-workflow ( LTRHARVEST+LTRFINDER -> LTRRETRIEVER ) takes ~2.5 hours of compute time. LAI estimates for four plant assemblies are listed below. + +| Assembly | EDTA_LAI | FASTA_LTRRETRIEVER_LAI | +| ----------- | -------- | ---------------------- | +| ck6901m/v2 | 18.43 | 16.19 | +| donghong/v1 | 19.03 | 16.85 | +| red5/v2.1 | 18.75 | 16.59 | +| tair/v10 | 18.06 | 17.42 | + +## v1.1 [09-Nov-2023] + +1. Now running kraken2 with a single cpu. +2. Now pulling containers from https://depot.galaxyproject.org/singularity/ + +## v1.0.1 [07-Sep-2023] + +1. Now pipeline timeline, report, and trace are enabled by default. +2. Included `procps` package where needed to allow NextFlow to collect system statistics. + +## v1 [25-Jul-2023] + +Same as v1rc6c + +## v1rc6c [20-Jul-2023] + +1. Added logic for the `-mono` parameter in LAI. This parameter allows correct LAI calculation for polyploid assemblies. +2. Fixed the typo in `assemblathon_stats` in nextflow.config. +3. Fixed the test_full.config example config and docs to exclude the mitochondrion genome from synteny and LAI modules. +4. Now saving `*.EDTA.TEanno.gff3` and `*.EDTA.intact.gff3` with original fasta ids. +5. Removed comments from the ID lines of the FASTA file before running lAI. +6. Now presenting the PARAMS page as formatted JSON rather than a table. +7. Now SAMBLASTER can run up to 20 hours. +8. (RC6b) NCBI FCS GX taxonomy is now presented as a Krona plot. (RC6c) No hits are included. Sequence length is used when calculating abundance. +9. (RC6c) Krona plot for Kraken2 now uses sequence length for abundance calculation. +10. Made ASSEMBLATHON_STATS robust to missing paths declared in the PATH variable. + +## v1rc5 [22-Jun-2023] + +1. Updated README in accordance with SPO Editor. +2. Added a note on LTR sequence identity in the nextflow.config. +3. Split MATLOCK_BAM2_JUICER module into MATLOCK_BAM2_JUICER and JUICER_SORT and using `--parallel` with `sort`. + +## v1rc4 [15-Jun-2023] + +1. Fixed a bug in the BIOCODE GFF3 STATS module which resulted in a cramped up plot of CDS vs mRNA counts. + +## v1rc3 [14-Jun-2023] + +1. Fixed a bug in the BIOCODE GFF3 STATS module which prevented it from processing valid gff3 files. + +## v1rc2 [13-Jun-2023] + +1. Added labels to the pipeline flowchart. +2. Update the README based on team feedback. + +## v1rc1 [12-Jun-2023] + +1. Added validation for fasta and gff3 files. +2. Added support for compressed files (fasta.gz, gff3.gz). +3. Added BIOCODE GFF3 STATS. +4. Added correspondence checks between gff3 and fasta files. +5. Now using standard mode as default for LAI. +6. Added information regarding LAI:EDTA time requirements for various genome sizes. +7. Added information regarding influence of LAI:EDTA:is_sensitive flag on LAI scores. +8. Added a params summary page. +9. Now the default config file (nextflow.config) is designed to run out-of-the-box at PFR. There is no need to do any setup. +10. "report" is now the default results folder. +11. Added documentation and configuration files for examples based on publicly accessible data from NCBI. +12. Added test configurations for Fungal, Bacterial, and Viral assemblies. +13. Added test configuration for a Transcriptome of a Nematode. +14. Now allowed up to 7 days for SYNTENY::DNADIFF based on recent evidence from two ~2.5 GB genomes. + +## v0.10.9 [01-Jun-2023] + +1. CRITICAL: Fixed a bug in LAI::EDTA which prevented it from renaming fasta ids in case they were longer than 13 characters. + +## v0.10.8 [30-May-2023] + +1. Now NCBI FCS Adaptor and NCBI FCS GX both run in parallel so that both contamination checks are part of the final report even if there is adaptor contamination. + +## v0.10.7 [29-May-2023] + +1. CRITICAL: Fixed a bug in LAI::EDTA which prevented it from renaming fasta ids in case they were longer than 13 characters. +2. Now the HiC module does not require the storage_server parameter and the HiC contact map does not disappear when the report is moved across folders. +3. Further developed the tutorials section. +4. Improved presentation of tables for BUSCO and LAI in the report. + +## v0.10.6 [25-May-2023] + +1. CRITICAL: Fixed a bug in LAI::EDTA which prevented it from renaming fasta ids in case they were longer than 13 characters. +2. CRITICAL: Fixed a bug in LAI::EDTA which prevented it from accessing the tmp directory. +3. BREAKING: Merged the max_resources config file into the main config file. Slight modifications are required when using the same config file across versions. +4. Now using a central location for assembly_qc singularity containers (/workspace/assembly_qc/singularity) so that individual users don't have to download these containers. +5. Increased resources for the nextflow process so that it can run child processes effectively. +6. Now using nf-core's convention for resource allocation and error strategy. +7. Removed the option to enable hyper-threading. +8. Now only saving the renamed.ids.tsv instead of the whole fasta file from EDTA. +9. Now also saving the EDTA.intact.gff3 file as EDTA sometimes does not store all the annotations in the EDTA.TEanno.gff3 file. + +## v0.10.5 [19-May-2023] + +1. CRITICAL: Fixed a bug in RUN_ASSEMBLY_VISUALIZER, HIC_QC introduced by the specification of the temporary directory in version 0.10.4. +2. MATLOCK_BAM2_JUICER now has two hours time limit. +3. Removed dependency on conda. Instead the pipeline now requires vanilla python > 3.7. No specific python packages are required. +4. Started adding detailed tutorials. +5. Now TIDK supports a filter by size parameter to filter out small contigs from it output. By default this filter is turned off. + +## v0.10.4 [16-May-2023] + +1. Moved the main workflow into `workflows/assembly_qc.nf` so that it can be imported by other NextFlow pipelines. +2. Fixed a bug in synteny due to which the pipeline did not resume properly sometimes. +3. The included binaries now have unique versions to avoid collision with binaries with same names already present on local PATH. +4. Now using a unique name for the conda environment to have better interoperability across pipelines. +5. Merged configuration files for compiled and max_resources. +6. CRITICAL: Now explicitly setting the temporary directory to avoid "No space left" errors. This problem may have affected container build and NCBI FCS Adaptor/GX modules in the past. +7. Now reporting max_gap and min_bundle size in the report for improved readability. + +## v0.10.3 [08-May-2023] + +1. Improved annotation of the config file. +2. Now using natural sort in the synteny color generator so that chr10's color is assigned after chr9's color. +3. Removed global variable definitions in the synteny module in the hope of improving resume-ability. +4. Now all the processes have unique tags. This ensures traceability and resume-ability. +5. CRITICAL: Fixed a bug in the HIC module due to which the pipeline failed to resume properly in some cases. This bug may have also caused mislabelling of the output hic file such that `hap1.hic` may be labelled as `hap2.hic` and vice versa. +6. Added GPLv3 license. +7. Now assembly tags in the dropdown menus of the report are in natural sort order. + +## v0.10.2 [04-May-2023] + +1. Allowed 2 hours for DNADIFF and CIRCOS_BUNDLE_LINKS modules. +2. Contigs are now ordered by number on the synteny plot. +3. Added `color_by_contig` option to the synteny module along with a maximum contrast color generator. + +## v0.10.1 [28-April-2023] + +1. Fixed a bug in the TIDK module which resulted in genome fasta file emptying in some cases. +2. Added a contributors section to README.md +3. Generalized and simplified configuration parameters and annotations. +4. Fixed a bug in synteny analysis where `between_target_asm` flag had no effect. +5. Updated Juicebox.js to 2.4.3 so that HIC module works behind a VPN. +6. Sorted the list of synteny plots. +7. Removed auto-capitalization of text in the first column of report tables. +8. Fixed a bug in the synteny module which resulted in incorrect inclusion of target sequences in 1-vs-all synteny maps. +9. In the synteny plot, label font size and ticks are now responsive to the number of sequences. +10. Added the `plot_1_vs_all` option in the synteny module. +11. Added `max_gap` and `min_bundle_size` options to the synteny module. + +## v0.10 [20-April-2023] + +1. Added Synteny Analysis. +2. Added "-q" and "-qq" option to LAI. "-qq" is the default. +3. Now copying the \*.TElib.fa file from EDTA work dir to the results folder. +4. Fixed the n_limit bug in assemblathon_stats.pl. +5. Now using 4-hour time limit for FASTP and FASTQC. +6. Added references for all the tools in the README. +7. Now the conda environment is saved in the users home directory so that it can be shared across pipeline runs. +8. Updated Juicebox.js to 2.4.1. +9. Allowed 8 hours for BWA MEM. +10. Fixed a bug in LAI where the output was not parsed correctly due to file name mismatch. + +## v0.9 [31-Mar-2023] + +1. Added NCBI FCS GX module. +2. Added additional annotation to config file. +3. Removed unnecessary species argument in BUSCO module. +4. Moved NCBI FCS Adaptor/GX scripts to user home directory for sharing across pipeline downloads to different directories. + +## v0.8 [29-Mar-2023] + +1. Now using system-wide DBs for BUSCO and KRAKEN2. +2. Added HiC Contact Map module. +3. Further simplified and annotated the config file. + +## v0.7.2 [24-Mar-2023] + +1. Fixed a potential bug in ncbi fcs adaptor. +2. Fixed rm -f bug in KRAKEN2. +3. Added additional info for LAI +4. Fixed a few typos in the config file. + +## v0.7.1 [23-Mar-2023] + +1. Fixed a bug in the slurm job submission script. +2. Fixed a bug in the ASSEMBLATHON_STATS module. +3. Fixed a bug in SETUP_KRAKEN2_DB module. +4. Now using uniform naming in the TIDK sub-workflow. +5. Max time for LAI now set to 2 hours. + +## v0.7 [17-Mar-2023] + +1. Added Kraken2 and NCBI FCS Adaptor tools. +2. Added Assemblathon stats. +3. Added `Genometools gt stat` statistics for gff3 files. +4. Added both a priori and a posteriori sequence search in TIDK. +5. Simplified pipeline flow chart. +6. Simplified conda environment. +7. Fixed css styling browser conflicts +8. TIDK process now uses a container instead of conda. + +## v0.6.1 [8-Mar-2023] + +1. Included results_dict and dependencies dict (without html formatting) to json. +2. Removed completed items in readme. +3. Fixed json dump repeating image url. + +## v0.6 [17-Feb-2023] + +1. Added LAI. +2. Now sorting sequences by size before feeding to TIDK. +3. Added skip switches for all the tools. +4. Added configuration annotations. +5. Optimised resource allocation. + +## v0.5.1 + +1. Changed report parsers to allow alphanumeric ([a-zA-Z0-9_]) characters in the haplotype names. + +## v0.5 + +1. Added TIDK + +## v0.4 + +1. Added ability run BUSCO for multiple augustus species simultaneously +2. Formatted tabs into a drop down list for ease of navigation +3. Summary page has been added +4. BUSCO plots are now rendered on the summary page +5. Styling has been changed for better user experience + +## v0.3 + +1. Added ability to run BUSCO for multiple haplotypes simultaneously +2. Updated README for new functionality +3. Adjusted styling for easier comparisons between reports +4. Incorporated conda instead of python venv + +## v0.2 + +1. Added ability to run BUSCO for multiple lineages simultaneously +2. Removed intermediary outputDir +3. Standardised naming conventions across the tool +4. Updated README for new functionality +5. 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Mostly used to configure linting of bin/check_samplesheet.py with Ruff. +# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff. # Should be kept the same as nf-core/tools to avoid fighting with template synchronisation. [tool.ruff] line-length = 120 From 2f8b46ad35cc25c48cb453f9f55fe15e4229a53e Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 20 Feb 2024 16:48:26 +1300 Subject: [PATCH 14/65] Bumped nxf version to 23.04.0 --- .github/workflows/ci.yml | 2 +- README.md | 2 +- nextflow.config | 2 +- 3 files changed, 3 insertions(+), 3 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 1e763b3d..9e9668cc 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -24,7 +24,7 @@ jobs: strategy: matrix: NXF_VER: - - "22.04.3" + - "23.04.0" - "latest-everything" steps: - name: Check out pipeline code diff --git a/README.md b/README.md index da8711ce..ee85285e 100644 --- a/README.md +++ b/README.md @@ -1,7 +1,7 @@ [![GitHub Actions CI Status](https://github.com/plant-food-research-open/assemblyqc/workflows/nf-core%20CI/badge.svg)](https://github.com/plant-food-research-open/assemblyqc/actions?query=workflow%3A%22nf-core+CI%22) [![GitHub Actions Linting Status](https://github.com/plant-food-research-open/assemblyqc/workflows/nf-core%20linting/badge.svg)](https://github.com/plant-food-research-open/assemblyqc/actions?query=workflow%3A%22nf-core+linting%22)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.10647870-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.10647870) -[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A522.04.3-23aa62.svg)](https://www.nextflow.io/) +[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) diff --git a/nextflow.config b/nextflow.config index 1af86d10..ec565e3c 100644 --- a/nextflow.config +++ b/nextflow.config @@ -260,7 +260,7 @@ manifest { homePage = 'https://github.com/plant-food-research-open/assemblyqc' description = """A NextFlow pipeline which evaluates assembly quality with well-established tools and presents the results in a unified html report.""" mainScript = 'main.nf' - nextflowVersion = '!>=22.04.3' + nextflowVersion = '!>=23.04.0' version = '1.4dev' doi = '10.5281/zenodo.10647870' } From 74d9ea7560df793902df99b6d459aaf4c897cee2 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 20 Feb 2024 17:23:27 +1300 Subject: [PATCH 15/65] Updated pipeline upto gt/stat --- README.md | 14 +- conf/modules.config | 221 ++++++++++++++++++ modules.json | 35 +++ .../environment.yml | 9 + .../checkgff3fastacorrespondence/main.nf | 25 ++ .../checkgff3fastacorrespondence/meta.yml | 56 +++++ .../check_gff3_fasta_correspondence.sh | 70 ++++++ .../tests/main.nf.test | 70 ++++++ .../tests/main.nf.test.snap | 72 ++++++ .../tests/tags.yml | 2 + modules/pfr/gt/gff3/environment.yml | 9 + modules/pfr/gt/gff3/main.nf | 51 ++++ modules/pfr/gt/gff3/meta.yml | 48 ++++ modules/pfr/gt/gff3/tests/main.nf.test | 61 +++++ modules/pfr/gt/gff3/tests/main.nf.test.snap | 72 ++++++ modules/pfr/gt/gff3/tests/nextflow.config | 3 + modules/pfr/gt/gff3/tests/tags.yml | 2 + modules/pfr/gt/gff3validator/environment.yml | 9 + modules/pfr/gt/gff3validator/main.nf | 50 ++++ modules/pfr/gt/gff3validator/meta.yml | 49 ++++ .../pfr/gt/gff3validator/tests/main.nf.test | 63 +++++ .../gt/gff3validator/tests/main.nf.test.snap | 72 ++++++ modules/pfr/gt/gff3validator/tests/tags.yml | 2 + modules/pfr/gt/stat/environment.yml | 9 + modules/pfr/gt/stat/main.nf | 35 +++ modules/pfr/gt/stat/meta.yml | 46 ++++ modules/pfr/gt/stat/tests/main.nf.test | 37 +++ modules/pfr/gt/stat/tests/main.nf.test.snap | 31 +++ modules/pfr/gt/stat/tests/tags.yml | 2 + subworkflows/pfr/gff3_validate/main.nf | 61 +++++ subworkflows/pfr/gff3_validate/meta.yml | 50 ++++ .../pfr/gff3_validate/tests/main.nf.test | 86 +++++++ .../pfr/gff3_validate/tests/main.nf.test.snap | 115 +++++++++ .../pfr/gff3_validate/tests/nextflow.config | 6 + subworkflows/pfr/gff3_validate/tests/tags.yml | 2 + workflows/assemblyqc.nf | 33 ++- 36 files changed, 1566 insertions(+), 12 deletions(-) create mode 100644 modules/pfr/custom/checkgff3fastacorrespondence/environment.yml create mode 100644 modules/pfr/custom/checkgff3fastacorrespondence/main.nf create mode 100644 modules/pfr/custom/checkgff3fastacorrespondence/meta.yml create mode 100755 modules/pfr/custom/checkgff3fastacorrespondence/templates/check_gff3_fasta_correspondence.sh create mode 100644 modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test create mode 100644 modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test.snap create mode 100644 modules/pfr/custom/checkgff3fastacorrespondence/tests/tags.yml create mode 100644 modules/pfr/gt/gff3/environment.yml create mode 100644 modules/pfr/gt/gff3/main.nf create mode 100644 modules/pfr/gt/gff3/meta.yml create mode 100644 modules/pfr/gt/gff3/tests/main.nf.test create mode 100644 modules/pfr/gt/gff3/tests/main.nf.test.snap create mode 100644 modules/pfr/gt/gff3/tests/nextflow.config create mode 100644 modules/pfr/gt/gff3/tests/tags.yml create mode 100644 modules/pfr/gt/gff3validator/environment.yml create mode 100644 modules/pfr/gt/gff3validator/main.nf create mode 100644 modules/pfr/gt/gff3validator/meta.yml create mode 100644 modules/pfr/gt/gff3validator/tests/main.nf.test create mode 100644 modules/pfr/gt/gff3validator/tests/main.nf.test.snap create mode 100644 modules/pfr/gt/gff3validator/tests/tags.yml create mode 100644 modules/pfr/gt/stat/environment.yml create mode 100644 modules/pfr/gt/stat/main.nf create mode 100644 modules/pfr/gt/stat/meta.yml create mode 100644 modules/pfr/gt/stat/tests/main.nf.test create mode 100644 modules/pfr/gt/stat/tests/main.nf.test.snap create mode 100644 modules/pfr/gt/stat/tests/tags.yml create mode 100644 subworkflows/pfr/gff3_validate/main.nf create mode 100644 subworkflows/pfr/gff3_validate/meta.yml create mode 100644 subworkflows/pfr/gff3_validate/tests/main.nf.test create mode 100644 subworkflows/pfr/gff3_validate/tests/main.nf.test.snap create mode 100644 subworkflows/pfr/gff3_validate/tests/nextflow.config create mode 100644 subworkflows/pfr/gff3_validate/tests/tags.yml diff --git a/README.md b/README.md index ee85285e..7e9252fb 100644 --- a/README.md +++ b/README.md @@ -69,12 +69,14 @@ flowchart LR Prepare an `assemblysheet.csv` file with following columns representing target assemblies and associated meta-data. -- tag: A unique tag which represents the target assembly throughout the pipeline and in the final report -- fasta: FASTA file -- gff3 [Optional]: GFF3 annotation file if available -- monoploid_ids [Optional]: A txt file listing the IDs used to calculate LAI in monoploid mode if necessary -- hic_reads [Optional] A SRA id such as 'SRR8238190' or path to paired reads such as 'PG_PETUNIA_HiC_CGYCF_CACTCA_L001_R{1,2}.fastq.gz' -- synteny_labels [Optional]: A two column tsv file listing fasta sequence ids (first column) and labels for the synteny plots (second column) when performing synteny analysis +- `tag:` A unique tag which represents the target assembly throughout the pipeline and in the final report +- `fasta:` FASTA file +- `gff3 [Optional]:` GFF3 annotation file if available +- `monoploid_ids [Optional]:` A txt file listing the IDs used to calculate LAI in monoploid mode if necessary +- `hic_reads [Optional]:` A SRA id such as 'SRR8238190' or path to paired reads such as 'PG_PETUNIA_HiC_CGYCF_CACTCA_L001_R{1,2}.fastq.gz' +- `synteny_labels [Optional]:` A two column tsv file listing fasta sequence ids (first column) and labels for the synteny plots (second column) when performing synteny analysis + +See a minimal example [assemblysheet.csv](./assets/assemblysheet.csv) Now, you can run the pipeline using: diff --git a/conf/modules.config b/conf/modules.config index bb1fc48d..26190d94 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -21,3 +21,224 @@ process { } } + +process { + withName: ASSEMBLATHON_STATS { + publishDir = [ + path: { "${params.outdir}/assemblathon_stats" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: NCBI_FCS_ADAPTOR { + publishDir = [ + path: { "${params.outdir}/ncbi_fcs_adaptor" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: RUN_BUSCO { + publishDir = [ + path: { "${params.outdir}/busco" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: RUN_KRAKEN2 { + publishDir = [ + path: { "${params.outdir}/kraken2" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: NCBI_FCS_GX_SCREEN_SAMPLES { + publishDir = [ + path: { "${params.outdir}/ncbi_fcs_gx" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: CIRCOS { + publishDir = [ + path: { "${params.outdir}/synteny/${target_on_ref_seq}" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } +} + + +process { + withName: '.*:GFF3_VALIDATE:GT_GFF3' { + ext.args = '-tidy -retainids' + } + + withName: GT_STAT { + ext.args = [ + '-genelengthdistri', + '-genescoredistri', + '-exonlengthdistri', + '-exonnumberdistri', + '-intronlengthdistri', + '-cdslengthdistri', + '-addintrons' + ].join(' ').trim() + + publishDir = [ + path: { "${params.outdir}/genometools_gt_stat" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } +} + +process { + + withName: FILTER_BY_LENGTH { + ext.args = params.tidk_filter_by_size ? "-m ${params.tidk_filter_size_bp}" : '' + ext.prefix = { "${meta.id}.filtered" } + } + + withName: SORT_BY_LENGTH { + ext.args = '--quiet --reverse --by-length' + ext.prefix = { "${meta.id}.sorted" } + } + + withName: TIDK_EXPLORE { + ext.args = '--minimum 5 --maximum 30' + publishDir = [ + path: { "${params.outdir}/tidk" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: TIDK_SEARCH_APRIORI { + ext.prefix = { "${meta.id}.apriori" } + ext.args = '--extension tsv' + publishDir = [ + path: { "${params.outdir}/tidk" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: TIDK_SEARCH_APOSTERIORI { + ext.prefix = { "${meta.id}.aposteriori" } + ext.args = '--extension tsv' + publishDir = [ + path: { "${params.outdir}/tidk" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: TIDK_PLOT_APRIORI { + ext.prefix = { "${meta.id}.apriori" } + publishDir = [ + path: { "${params.outdir}/tidk" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: TIDK_PLOT_APOSTERIORI { + ext.prefix = { "${meta.id}.aposteriori" } + publishDir = [ + path: { "${params.outdir}/tidk" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } +} + + +process { + + withName: CUSTOM_SHORTENFASTAIDS { + publishDir = [ + path: { "${params.outdir}/lai" }, + mode: params.publish_dir_mode, + pattern: '*.short.ids.tsv' + ] + } + + withName: EDTA_LTRHARVEST { + ext.prefix = { "${meta.id}_edta_ltrharvest" } + } + + withName: LTRFINDER { + ext.args = '-harvest_out -size 1000000 -time 300' + } + + withName: CAT_CAT { + ext.prefix = { "${meta.id}_ltrharvest_ltrfinder.tabout" } + } + + withName: LTRRETRIEVER { + publishDir = [ + path: { "${params.outdir}/lai" }, + mode: params.publish_dir_mode, + pattern: '*.LTRlib.fa' + ] + } + + withName: CUSTOM_RESTOREGFFIDS { + publishDir = [ + path: { "${params.outdir}/lai" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: LAI { + publishDir = [ + path: { "${params.outdir}/lai" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } +} + +process { + + withName: FASTQC_RAW { + publishDir = [ + path: { "${params.outdir}/hic/fastqc_raw" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: FASTQC_TRIM { + publishDir = [ + path: { "${params.outdir}/hic/fastqc_trim" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: FASTP { + ext.args = params.hic_fastp_ext_args + publishDir = [ + path: { "${params.outdir}/hic/fastp" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: BWA_MEM { + ext.prefix = { "${meta.id}.on.${meta.ref_id}.bwa.mem" } + ext.args = '-5SP' + } + + withName: SAMBLASTER { + ext.prefix = { "${meta.id}.on.${meta.ref_id}.samblaster" } + ext.args3 = '-h -F 2316' + } +} diff --git a/modules.json b/modules.json index f01155f8..ef57949b 100644 --- a/modules.json +++ b/modules.json @@ -2,6 +2,41 @@ "name": "plant-food-research-open/assemblyqc", "homePage": "https://github.com/plant-food-research-open/assemblyqc", "repos": { + "git@github.com:PlantandFoodResearch/nxf-modules.git": { + "modules": { + "pfr": { + "custom/checkgff3fastacorrespondence": { + "branch": "main", + "git_sha": "1a76f884082c786760559c462063a5d1de94ca83", + "installed_by": ["gff3_validate"] + }, + "gt/gff3": { + "branch": "main", + "git_sha": "bfa4874d3942bdff70cb8df17322834125cafb28", + "installed_by": ["gff3_validate"] + }, + "gt/gff3validator": { + "branch": "main", + "git_sha": "889b9b57b611dcb063594608c2a222c928327cba", + "installed_by": ["gff3_validate"] + }, + "gt/stat": { + "branch": "main", + "git_sha": "cb5fb0be78a98fd1e32b7c90d6adf8c3bf44133e", + "installed_by": ["modules"] + } + } + }, + "subworkflows": { + "pfr": { + "gff3_validate": { + "branch": "main", + "git_sha": "f9b96bf8142a01f0649ff90570fb10aa973504b9", + "installed_by": ["subworkflows"] + } + } + } + }, "https://github.com/nf-core/modules.git": { "modules": { "nf-core": { diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/environment.yml b/modules/pfr/custom/checkgff3fastacorrespondence/environment.yml new file mode 100644 index 00000000..ec0e86d1 --- /dev/null +++ b/modules/pfr/custom/checkgff3fastacorrespondence/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "custom_checkgff3fastacorrespondence" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::samtools=1.18" diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/main.nf b/modules/pfr/custom/checkgff3fastacorrespondence/main.nf new file mode 100644 index 00000000..c1abb6f4 --- /dev/null +++ b/modules/pfr/custom/checkgff3fastacorrespondence/main.nf @@ -0,0 +1,25 @@ +process CUSTOM_CHECKGFF3FASTACORRESPONDENCE { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1': + 'biocontainers/samtools:1.18--h50ea8bc_1' }" + + input: + tuple val(meta), path(gff3) + path(fasta) + + output: + tuple val(meta), path('*.success.log') , emit: success_log , optional: true + tuple val(meta), path('*.error.log') , emit: error_log , optional: true + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + shell: + prefix = task.ext.prefix ?: "${meta.id}" + template 'check_gff3_fasta_correspondence.sh' +} diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/meta.yml b/modules/pfr/custom/checkgff3fastacorrespondence/meta.yml new file mode 100644 index 00000000..69bbd053 --- /dev/null +++ b/modules/pfr/custom/checkgff3fastacorrespondence/meta.yml @@ -0,0 +1,56 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "custom_checkgff3fastacorrespondence" +description: "A custom bash script which checks the correspondence of a gff3 file with a fasta file" +keywords: + - genome + - gff3 + - annotation + - validation +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: http://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 + licence: ["MIT"] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - gff3: + type: file + description: Input gff3 file + pattern: "*.{gff,gff3}" + - fasta: + type: file + description: Input fasta file + pattern: "*.{fsa,fa,fasta}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - success_log: + type: file + description: Log file for successful validation + pattern: "*.success.log" + - error_log: + type: file + description: Log file for failed validation + pattern: "*.error.log" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/templates/check_gff3_fasta_correspondence.sh b/modules/pfr/custom/checkgff3fastacorrespondence/templates/check_gff3_fasta_correspondence.sh new file mode 100755 index 00000000..611c64b3 --- /dev/null +++ b/modules/pfr/custom/checkgff3fastacorrespondence/templates/check_gff3_fasta_correspondence.sh @@ -0,0 +1,70 @@ +#!/usr/bin/env bash + +# Bump VERSION on edit +VERSION="v1" + +gff3_file="!{gff3}" +fasta_file="!{fasta}" +out_prefix="!{prefix}" +task_process="!{task.process}" + +# Record versions +cat <<-END_VERSIONS > versions.yml +"${task_process}": + samtools: $(echo $(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*$//' ) +END_VERSIONS + +# Requires +# samtools faidx + +## STEP 1 +# Check that gff3 has no identifers that are not in fasta (fasta can +# have ids that are not in gff3 since not all assembly units have gff3 records + +# Extract identifiers from the GFF3 file +gff3_identifiers=$(grep -v '^#' "$gff3_file" | awk '{print $1}' | sort -u) + +# Extract identifiers from the FASTA file +fasta_identifiers=$(grep '^>' "$fasta_file" | awk '{print substr($1, 2)}' | sort -u) + +# Compare identifiers and find any that are present in the GFF3 but not in the FASTA +missing_identifiers=$(comm -23 <(echo "$gff3_identifiers") <(echo "$fasta_identifiers")) + +# Check if any missing identifiers were found +if [[ -n "$missing_identifiers" ]]; then + touch "${out_prefix}.error.log" + echo "Failed to validate gff3 file for: $tag_label" >> "${out_prefix}.error.log" + echo "Fasta file: $fasta_file" >> "${out_prefix}.error.log" + echo "Gff3 file: $gff3_file" >> "${out_prefix}.error.log" + echo "GFF3 file contains identifiers not present in FASTA:" >> "${out_prefix}.error.log" + echo "$missing_identifiers" >> "${out_prefix}.error.log" + exit 0 +fi + +## STEP 2 +# check that there are no coordiantes in gff3 for any seqid that are +# greater than the seq length of the paretn fasta entry + +# Compute sequence lengths using samtools faidx +samtools faidx "$fasta_file" | cut -f 1,2 > sequence_lengths.txt + +# Check GFF3 file for coordinates exceeding sequence lengths +while IFS=$'\t' read -r seqname source feature start end score strand frame attributes && \ + read -r seq seq_length <&3; do + if [[ $start -gt $seq_length || $end -gt $seq_length ]]; then + touch "${out_prefix}.error.log" + echo "Failed to validate gff3 file for: $tag_label" >> "${out_prefix}.error.log" + echo "Fasta file: $fasta_file" >> "${out_prefix}.error.log" + echo "Gff3 file: $gff3_file" >> "${out_prefix}.error.log" + echo "Coordinates exceed sequence length in GFF3 file:" >> "${out_prefix}.error.log" + echo "Sequence: $seqname" >> "${out_prefix}.error.log" + echo "Sequence length: $seq_length" >> "${out_prefix}.error.log" + echo "Start: $start" >> "${out_prefix}.error.log" + echo "End: $end" >> "${out_prefix}.error.log" + exit 0 + fi +done < "$gff3_file" 3< "sequence_lengths.txt" + +touch "${out_prefix}.success.log" +echo "All tests passed..." >> "${out_prefix}.success.log" +exit 0 diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test b/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test new file mode 100644 index 00000000..91578e5b --- /dev/null +++ b/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test @@ -0,0 +1,70 @@ +nextflow_process { + + name "Test Process CUSTOM_CHECKGFF3FASTACORRESPONDENCE" + script "../main.nf" + process "CUSTOM_CHECKGFF3FASTACORRESPONDENCE" + + tag "modules" + tag "modules_nfcore" + tag "custom" + tag "custom/checkgff3fastacorrespondence" + + test("sarscov2-fasta-gff3-success") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) + ] + + input[1] = [ + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.error_log == [] }, + { assert process.out.success_log != null }, + { assert path(process.out.success_log.get(0).get(1)).getText().contains("All tests passed...")}, + ) + } + + } + + test("sarscov2-gff3-homo_sapiens-fasta-error") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) + ] + + input[1] = [ + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.success_log == [] }, + { assert process.out.error_log != null }, + { assert path(process.out.error_log.get(0).get(1)).getText().contains("GFF3 file contains identifiers not present in FASTA")}, + ) + } + + } + +} diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test.snap b/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test.snap new file mode 100644 index 00000000..261e0dc3 --- /dev/null +++ b/modules/pfr/custom/checkgff3fastacorrespondence/tests/main.nf.test.snap @@ -0,0 +1,72 @@ +{ + "sarscov2-gff3-homo_sapiens-fasta-error": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "test" + }, + "test.error.log:md5,8a119170625dc95fb2faa6843fad2c3f" + ] + ], + "2": [ + "versions.yml:md5,c8e0bb60f7422aa6c15db35013620802" + ], + "error_log": [ + [ + { + "id": "test" + }, + "test.error.log:md5,8a119170625dc95fb2faa6843fad2c3f" + ] + ], + "success_log": [ + + ], + "versions": [ + "versions.yml:md5,c8e0bb60f7422aa6c15db35013620802" + ] + } + ], + "timestamp": "2023-11-29T12:24:08.677505" + }, + "sarscov2-fasta-gff3-success": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.success.log:md5,5cad27984e6af4889f7dcf12264fe47b" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,c8e0bb60f7422aa6c15db35013620802" + ], + "error_log": [ + + ], + "success_log": [ + [ + { + "id": "test" + }, + "test.success.log:md5,5cad27984e6af4889f7dcf12264fe47b" + ] + ], + "versions": [ + "versions.yml:md5,c8e0bb60f7422aa6c15db35013620802" + ] + } + ], + "timestamp": "2023-11-29T12:24:04.530428" + } +} \ No newline at end of file diff --git a/modules/pfr/custom/checkgff3fastacorrespondence/tests/tags.yml b/modules/pfr/custom/checkgff3fastacorrespondence/tests/tags.yml new file mode 100644 index 00000000..708130d8 --- /dev/null +++ b/modules/pfr/custom/checkgff3fastacorrespondence/tests/tags.yml @@ -0,0 +1,2 @@ +custom/checkgff3fastacorrespondence: + - "modules/pfr/custom/checkgff3fastacorrespondence/**" diff --git a/modules/pfr/gt/gff3/environment.yml b/modules/pfr/gt/gff3/environment.yml new file mode 100644 index 00000000..8289fb31 --- /dev/null +++ b/modules/pfr/gt/gff3/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "gt_gff3" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::genometools-genometools=1.6.5" diff --git a/modules/pfr/gt/gff3/main.nf b/modules/pfr/gt/gff3/main.nf new file mode 100644 index 00000000..d27e2bb9 --- /dev/null +++ b/modules/pfr/gt/gff3/main.nf @@ -0,0 +1,51 @@ +process GT_GFF3 { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/genometools-genometools:1.6.5--py310h3db02ab_0': + 'biocontainers/genometools-genometools:1.6.5--py310h3db02ab_0' }" + + input: + tuple val(meta), path(gff3) + + output: + tuple val(meta), path("*.gt.gff3") , emit: gt_gff3 , optional: true + tuple val(meta), path("*.error.log"), emit: error_log , optional: true + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + gt \\ + gff3 \\ + $args \\ + "$gff3" \\ + > "${prefix}.gt.gff3" \\ + 2> "${prefix}.error.log" \\ + || echo "Errors from gt-gff3 printed to ${prefix}.error.log" + + if grep -q "gt gff3: error:" "${prefix}.error.log"; then + echo "gt-gff3 failed to parse $gff3" + + rm \\ + "${prefix}.gt.gff3" + else + echo "gt-gff3 successfully parsed $gff3" + + mv \\ + "${prefix}.error.log" \\ + gt_gff3.stderr + fi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + genometools: \$(gt --version | head -1 | sed 's/gt (GenomeTools) //') + END_VERSIONS + """ +} diff --git a/modules/pfr/gt/gff3/meta.yml b/modules/pfr/gt/gff3/meta.yml new file mode 100644 index 00000000..5cecd8d0 --- /dev/null +++ b/modules/pfr/gt/gff3/meta.yml @@ -0,0 +1,48 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "gt_gff3" +description: "GenomeTools gt-gff3 utility to parse, possibly transform, and output GFF3 files" +keywords: + - genome + - gff3 + - annotation +tools: + - "gt": + description: "The GenomeTools genome analysis system" + homepage: "https://genometools.org/index.html" + documentation: "https://genometools.org/documentation.html" + tool_dev_url: "https://github.com/genometools/genometools" + doi: "10.1109/TCBB.2013.68" + licence: ["ISC"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - gff3: + type: file + description: Input gff3 file + pattern: "*.{gff,gff3}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - gt_gff3: + type: file + description: Parsed gff3 file produced only if there is no parsing error + pattern: "*.gt.gff3" + - error_log: + type: file + description: Error log if gt-gff3 failed to parse the input gff3 file + pattern: "*.error.log" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@gallvp" +maintainers: + - "@gallvp" diff --git a/modules/pfr/gt/gff3/tests/main.nf.test b/modules/pfr/gt/gff3/tests/main.nf.test new file mode 100644 index 00000000..cb44bc8f --- /dev/null +++ b/modules/pfr/gt/gff3/tests/main.nf.test @@ -0,0 +1,61 @@ +nextflow_process { + + name "Test Process GT_GFF3" + script "../main.nf" + process "GT_GFF3" + config "./nextflow.config" + + tag "modules" + tag "modules_nfcore" + tag "gt" + tag "gt/gff3" + + test("sarscov2-gff3-valid") { + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.gt_gff3 != null }, + { assert process.out.error_log == [] } + ) + } + + } + + test("sarscov2-gff3-invalid") { + when { + process { + """ + input[0] = Channel.of( + '##gff-version 3', + 'chr22\tID=gene:ENSG00000233995;Name=AP000547.1' + ) + .collectFile(name: 'sample.gff3', newLine: true) + .map { file -> [ [ id:'test' ], file ] } + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.gt_gff3 == [] }, + { assert process.out.error_log != null }, + { assert path(process.out.error_log.get(0).get(1)).getText().contains("gt gff3: error:") } + ) + } + } + +} diff --git a/modules/pfr/gt/gff3/tests/main.nf.test.snap b/modules/pfr/gt/gff3/tests/main.nf.test.snap new file mode 100644 index 00000000..f31e8d1c --- /dev/null +++ b/modules/pfr/gt/gff3/tests/main.nf.test.snap @@ -0,0 +1,72 @@ +{ + "sarscov2-gff3-invalid": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "test" + }, + "test.error.log:md5,31e6117c516f936ec403f792c732bc76" + ] + ], + "2": [ + "versions.yml:md5,9753770dd19a2a306dcf16d4aaf049eb" + ], + "error_log": [ + [ + { + "id": "test" + }, + "test.error.log:md5,31e6117c516f936ec403f792c732bc76" + ] + ], + "gt_gff3": [ + + ], + "versions": [ + "versions.yml:md5,9753770dd19a2a306dcf16d4aaf049eb" + ] + } + ], + "timestamp": "2023-11-28T13:43:34.620429" + }, + "sarscov2-gff3-valid": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.gt.gff3:md5,2ae900237ace415557b8735fac088b85" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,9753770dd19a2a306dcf16d4aaf049eb" + ], + "error_log": [ + + ], + "gt_gff3": [ + [ + { + "id": "test" + }, + "test.gt.gff3:md5,2ae900237ace415557b8735fac088b85" + ] + ], + "versions": [ + "versions.yml:md5,9753770dd19a2a306dcf16d4aaf049eb" + ] + } + ], + "timestamp": "2023-11-28T13:43:31.065832" + } +} \ No newline at end of file diff --git a/modules/pfr/gt/gff3/tests/nextflow.config b/modules/pfr/gt/gff3/tests/nextflow.config new file mode 100644 index 00000000..af562267 --- /dev/null +++ b/modules/pfr/gt/gff3/tests/nextflow.config @@ -0,0 +1,3 @@ +process { + ext.args = '-tidy -retainids' +} diff --git a/modules/pfr/gt/gff3/tests/tags.yml b/modules/pfr/gt/gff3/tests/tags.yml new file mode 100644 index 00000000..ae040309 --- /dev/null +++ b/modules/pfr/gt/gff3/tests/tags.yml @@ -0,0 +1,2 @@ +gt/gff3: + - "modules/pfr/gt/gff3/**" diff --git a/modules/pfr/gt/gff3validator/environment.yml b/modules/pfr/gt/gff3validator/environment.yml new file mode 100644 index 00000000..ea57ebe0 --- /dev/null +++ b/modules/pfr/gt/gff3validator/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "gt_gff3validator" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::genometools-genometools=1.6.5" diff --git a/modules/pfr/gt/gff3validator/main.nf b/modules/pfr/gt/gff3validator/main.nf new file mode 100644 index 00000000..ae7ec9e7 --- /dev/null +++ b/modules/pfr/gt/gff3validator/main.nf @@ -0,0 +1,50 @@ +process GT_GFF3VALIDATOR { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/genometools-genometools:1.6.5--py310h3db02ab_0': + 'biocontainers/genometools-genometools:1.6.5--py310h3db02ab_0' }" + + input: + tuple val(meta), path(gff3) + + output: + tuple val(meta), path('*.success.log') , emit: success_log , optional: true + tuple val(meta), path('*.error.log') , emit: error_log , optional: true + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + gt \\ + gff3validator \\ + "$gff3" \\ + > "${prefix}.success.log" \\ + 2> "${prefix}.error.log" \\ + || echo "Errors from gt-gff3validator printed to ${prefix}.error.log" + + if grep -q "input is valid GFF3" "${prefix}.success.log"; then + echo "Validation successful..." + + mv \\ + "${prefix}.error.log" \\ + gt_gff3validator.stderr + else + echo "Validation failed..." + + mv \\ + "${prefix}.success.log" \\ + gt_gff3validator.stdout + fi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + genometools: \$(gt --version | head -1 | sed 's/gt (GenomeTools) //') + END_VERSIONS + """ +} diff --git a/modules/pfr/gt/gff3validator/meta.yml b/modules/pfr/gt/gff3validator/meta.yml new file mode 100644 index 00000000..3322faf9 --- /dev/null +++ b/modules/pfr/gt/gff3validator/meta.yml @@ -0,0 +1,49 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "gt_gff3validator" +description: "GenomeTools gt-gff3validator utility to strictly validate a GFF3 file" +keywords: + - genome + - gff3 + - annotation + - validation +tools: + - "gt": + description: "The GenomeTools genome analysis system" + homepage: "https://genometools.org/index.html" + documentation: "https://genometools.org/documentation.html" + tool_dev_url: "https://github.com/genometools/genometools" + doi: "10.1109/TCBB.2013.68" + licence: ["ISC"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - gff3: + type: file + description: Input gff3 file + pattern: "*.{gff,gff3}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - success_log: + type: file + description: Log file for successful validation + pattern: "*.success.log" + - error_log: + type: file + description: Log file for failed validation + pattern: "*.error.log" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/pfr/gt/gff3validator/tests/main.nf.test b/modules/pfr/gt/gff3validator/tests/main.nf.test new file mode 100644 index 00000000..1b99e551 --- /dev/null +++ b/modules/pfr/gt/gff3validator/tests/main.nf.test @@ -0,0 +1,63 @@ +nextflow_process { + + name "Test Process GT_GFF3VALIDATOR" + script "../main.nf" + process "GT_GFF3VALIDATOR" + + tag "modules" + tag "modules_nfcore" + tag "gt" + tag "gt/gff3validator" + + test("custom-gff3-valid") { + + when { + process { + """ + input[0] = Channel.of( + '##gff-version 3', + 'chr22\thavana\tpseudogene\t16572027\t16574637\t.\t+\t.\tID=gene:ENSG00000233995;Name=AP000547.1' + ) + .collectFile(name: 'sample.gff3', newLine: true) + .map { file -> [ [ id:'test' ], file ] } + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.error_log == [] }, + { assert process.out.success_log != null }, + { assert path(process.out.success_log.get(0).get(1)).getText().contains("input is valid GFF3") } + ) + } + + } + + test("sarscov2-gff3-invalid") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_gff3'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.success_log == [] }, + { assert process.out.error_log != null }, + { assert path(process.out.error_log.get(0).get(1)).getText().contains("gt gff3validator: error:") } + ) + } + + } +} diff --git a/modules/pfr/gt/gff3validator/tests/main.nf.test.snap b/modules/pfr/gt/gff3validator/tests/main.nf.test.snap new file mode 100644 index 00000000..0b6f065a --- /dev/null +++ b/modules/pfr/gt/gff3validator/tests/main.nf.test.snap @@ -0,0 +1,72 @@ +{ + "sarscov2-gff3-invalid": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "test" + }, + "test.error.log:md5,c5d16b263a87072a13cca44fd811b8e2" + ] + ], + "2": [ + "versions.yml:md5,5927673eb73a8c22408643d224414215" + ], + "error_log": [ + [ + { + "id": "test" + }, + "test.error.log:md5,c5d16b263a87072a13cca44fd811b8e2" + ] + ], + "success_log": [ + + ], + "versions": [ + "versions.yml:md5,5927673eb73a8c22408643d224414215" + ] + } + ], + "timestamp": "2023-11-29T11:09:23.708792" + }, + "custom-gff3-valid": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.success.log:md5,b11ca5c18c865fc808ea0fef0b07da30" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,5927673eb73a8c22408643d224414215" + ], + "error_log": [ + + ], + "success_log": [ + [ + { + "id": "test" + }, + "test.success.log:md5,b11ca5c18c865fc808ea0fef0b07da30" + ] + ], + "versions": [ + "versions.yml:md5,5927673eb73a8c22408643d224414215" + ] + } + ], + "timestamp": "2023-11-29T11:09:19.530068" + } +} \ No newline at end of file diff --git a/modules/pfr/gt/gff3validator/tests/tags.yml b/modules/pfr/gt/gff3validator/tests/tags.yml new file mode 100644 index 00000000..e247d55e --- /dev/null +++ b/modules/pfr/gt/gff3validator/tests/tags.yml @@ -0,0 +1,2 @@ +gt/gff3validator: + - "modules/pfr/gt/gff3validator/**" diff --git a/modules/pfr/gt/stat/environment.yml b/modules/pfr/gt/stat/environment.yml new file mode 100644 index 00000000..dca959b3 --- /dev/null +++ b/modules/pfr/gt/stat/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "gt_stat" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::genometools-genometools=1.6.5" diff --git a/modules/pfr/gt/stat/main.nf b/modules/pfr/gt/stat/main.nf new file mode 100644 index 00000000..3308b562 --- /dev/null +++ b/modules/pfr/gt/stat/main.nf @@ -0,0 +1,35 @@ +process GT_STAT { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/genometools-genometools:1.6.5--py310h3db02ab_0': + 'biocontainers/genometools-genometools:1.6.5--py310h3db02ab_0' }" + + input: + tuple val(meta), path(gff3) + + output: + tuple val(meta), path("*.gt.stat.yml") , emit: stats + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + gt \\ + stat \\ + $args \\ + "$gff3" \\ + > "${prefix}.gt.stat.yml" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + genometools: \$(gt --version | head -1 | sed 's/gt (GenomeTools) //') + END_VERSIONS + """ +} diff --git a/modules/pfr/gt/stat/meta.yml b/modules/pfr/gt/stat/meta.yml new file mode 100644 index 00000000..203059a6 --- /dev/null +++ b/modules/pfr/gt/stat/meta.yml @@ -0,0 +1,46 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "gt_stat" +description: "GenomeTools gt-stat utility to show statistics about features contained in GFF3 files" +keywords: + - genome + - gff3 + - annotation + - statistics + - stats +tools: + - "gt": + description: "The GenomeTools genome analysis system" + homepage: "https://genometools.org/index.html" + documentation: "https://genometools.org/documentation.html" + tool_dev_url: "https://github.com/genometools/genometools" + doi: "10.1109/TCBB.2013.68" + licence: ["ISC"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - gff3: + type: file + description: Input gff3 file + pattern: "*.{gff,gff3}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - stats: + type: file + description: Stats file in yaml format + pattern: "*.gt.stat.yml" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/pfr/gt/stat/tests/main.nf.test b/modules/pfr/gt/stat/tests/main.nf.test new file mode 100644 index 00000000..57f5992c --- /dev/null +++ b/modules/pfr/gt/stat/tests/main.nf.test @@ -0,0 +1,37 @@ +nextflow_process { + + name "Test Process GT_STAT" + script "../main.nf" + process "GT_STAT" + + tag "modules" + tag "modules_nfcore" + tag "gt" + tag "gt/stat" + + test("sarscov2-gff3") { + + when { + process { + """ + input[0] = Channel.of( + "##gff-version 3" + + file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true).getText().toLowerCase() + ) + .collectFile(name: 'sample.gff3', newLine: true) + .map { file -> [ [ id:'test' ], file ] } + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert path(process.out.stats.get(0).get(1)).getText().contains("cdss: 12") } + ) + } + + } + +} diff --git a/modules/pfr/gt/stat/tests/main.nf.test.snap b/modules/pfr/gt/stat/tests/main.nf.test.snap new file mode 100644 index 00000000..2fcfb8a8 --- /dev/null +++ b/modules/pfr/gt/stat/tests/main.nf.test.snap @@ -0,0 +1,31 @@ +{ + "sarscov2-gff3": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.gt.stat.yml:md5,ebba7831ddbf916b8bbea675ba8693b5" + ] + ], + "1": [ + "versions.yml:md5,a184b50afb2ad6dd2d3d37b0a211dd71" + ], + "stats": [ + [ + { + "id": "test" + }, + "test.gt.stat.yml:md5,ebba7831ddbf916b8bbea675ba8693b5" + ] + ], + "versions": [ + "versions.yml:md5,a184b50afb2ad6dd2d3d37b0a211dd71" + ] + } + ], + "timestamp": "2023-11-29T11:34:48.057277" + } +} \ No newline at end of file diff --git a/modules/pfr/gt/stat/tests/tags.yml b/modules/pfr/gt/stat/tests/tags.yml new file mode 100644 index 00000000..46be6341 --- /dev/null +++ b/modules/pfr/gt/stat/tests/tags.yml @@ -0,0 +1,2 @@ +gt/stat: + - "modules/pfr/gt/stat/**" diff --git a/subworkflows/pfr/gff3_validate/main.nf b/subworkflows/pfr/gff3_validate/main.nf new file mode 100644 index 00000000..5437c5a6 --- /dev/null +++ b/subworkflows/pfr/gff3_validate/main.nf @@ -0,0 +1,61 @@ +include { GT_GFF3 } from '../../../modules/pfr/gt/gff3/main' +include { GT_GFF3VALIDATOR } from '../../../modules/pfr/gt/gff3validator/main' +include { CUSTOM_CHECKGFF3FASTACORRESPONDENCE } from '../../../modules/pfr/custom/checkgff3fastacorrespondence/main' + +workflow GFF3_VALIDATE { + + take: + ch_gff3 // channel: [ val(meta), gff3 ] + ch_fasta // channel: [ val(meta), fasta ] + + main: + + ch_versions = Channel.empty() + + // MODULE: GT_GFF3 + GT_GFF3 ( ch_gff3 ) + ch_versions = ch_versions.mix(GT_GFF3.out.versions.first()) + + // MODULE: GT_GFF3VALIDATOR + GT_GFF3VALIDATOR ( GT_GFF3.out.gt_gff3 ) + ch_versions = ch_versions.mix(GT_GFF3VALIDATOR.out.versions.first()) + + // MODULE: CUSTOM_CHECKGFF3FASTACORRESPONDENCE + GT_GFF3VALIDATOR.out.success_log + | join ( + GT_GFF3.out.gt_gff3 + ) + | map { meta, log, gff3 -> [ meta, gff3 ] } + | join ( + ch_fasta + ) + | set { ch_gff3_fasta } + + CUSTOM_CHECKGFF3FASTACORRESPONDENCE ( + ch_gff3_fasta.map { meta, gff3, fasta -> [ meta, gff3 ] }, + ch_gff3_fasta.map { meta, gff3, fasta -> fasta } + ) + + ch_versions = ch_versions.mix(CUSTOM_CHECKGFF3FASTACORRESPONDENCE.out.versions.first()) + + CUSTOM_CHECKGFF3FASTACORRESPONDENCE.out.success_log + | join ( + ch_gff3_fasta.map { meta, gff3, fasta -> [ meta, gff3 ] } + ) + | map { meta, log, gff3 -> [ meta, gff3 ] } + | set { ch_valid_gff3 } + + GT_GFF3.out.error_log + | mix ( + GT_GFF3VALIDATOR.out.error_log + ) + | mix ( + CUSTOM_CHECKGFF3FASTACORRESPONDENCE.out.error_log + ) + | set { ch_log_for_invalid_gff3 } + + emit: + valid_gff3 = ch_valid_gff3 // channel: [ val(meta), gff3 ] + log_for_invalid_gff3 = ch_log_for_invalid_gff3 // channel: [ val(meta), log ] + versions = ch_versions // channel: [ versions.yml ] +} diff --git a/subworkflows/pfr/gff3_validate/meta.yml b/subworkflows/pfr/gff3_validate/meta.yml new file mode 100644 index 00000000..5dea12a5 --- /dev/null +++ b/subworkflows/pfr/gff3_validate/meta.yml @@ -0,0 +1,50 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: gff3_validate +description: | + Validates a gff3 file using GenomeTools gt-gff3, gt-gff3validator and + checks its correspondence with a fasta file +keywords: + - genome + - gff3 + - annotation + - validation +components: + - gt/gff3 + - gt/gff3validator + - custom/checkgff3fastacorrespondence +input: + - ch_gff3: + type: file + description: | + Input channel containing a gff3 file + Structure: [ val(meta), path(gff3) ] + pattern: "*.{gff,gff3}" + - ch_fasta: + type: file + description: | + Input channel containing a fasta file + Structure: [ val(meta), path(fasta) ] + pattern: "*.{fsa,fa,fasta}" +output: + - valid_gff3: + type: file + description: | + Valid gff3 file + Structure: [ val(meta), path(gff3) ] + pattern: "*.gff3" + - log_for_invalid_gff3: + type: file + description: | + Error log if the gff3 file is invalid + Structure: [ val(meta), path(gff3) ] + pattern: "*.gff3" + - versions: + type: file + description: | + File containing software versions + Structure: [ path(versions.yml) ] + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/subworkflows/pfr/gff3_validate/tests/main.nf.test b/subworkflows/pfr/gff3_validate/tests/main.nf.test new file mode 100644 index 00000000..e71712b8 --- /dev/null +++ b/subworkflows/pfr/gff3_validate/tests/main.nf.test @@ -0,0 +1,86 @@ +nextflow_workflow { + + name "Test Workflow GFF3_VALIDATE" + script "../main.nf" + workflow "GFF3_VALIDATE" + config "./nextflow.config" + + tag "subworkflows" + tag "subworkflows_nfcore" + tag "subworkflows/gff3_validate" + tag "gff3_validate" + tag "gt" + tag "gt/gff3" + tag "gt/gff3validator" + tag "custom" + tag "custom/checkgff3fastacorrespondence" + + test("sarscov2-genome_gff3-genome_fasta-all_pass") { + + when { + workflow { + """ + input[0] = Channel.of([ [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) + ]) + input[1] = Channel.of([ [ id:'test' ], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ]) + """ + } + } + + then { + assertAll( + { assert workflow.success}, + { assert snapshot(workflow.out).match()} + ) + } + } + + test("homo_sapiens-genome_bed-genome_fasta-gt_gff3_fail") { + + when { + workflow { + """ + input[0] = Channel.of([ [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true) + ]) + input[1] = Channel.of([ [ id:'test' ], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ]) + """ + } + } + + then { + assertAll( + { assert workflow.success}, + { assert snapshot(workflow.out).match()} + ) + } + } + + test("sarscov2-genome_gff3-homo_sapiens-genome_fasta-correspondence_fail") { + + when { + workflow { + """ + input[0] = Channel.of([ [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) + ]) + input[1] = Channel.of([ [ id:'test' ], + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ]) + """ + } + } + + then { + assertAll( + { assert workflow.success}, + { assert snapshot(workflow.out).match()} + ) + } + } +} diff --git a/subworkflows/pfr/gff3_validate/tests/main.nf.test.snap b/subworkflows/pfr/gff3_validate/tests/main.nf.test.snap new file mode 100644 index 00000000..4d2a59b2 --- /dev/null +++ b/subworkflows/pfr/gff3_validate/tests/main.nf.test.snap @@ -0,0 +1,115 @@ +{ + "sarscov2-genome_gff3-genome_fasta-all_pass": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.gt.gff3:md5,2ae900237ace415557b8735fac088b85" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,10fe5c201e5fcddb52c3607ab3fdfb34", + "versions.yml:md5,856745cef2fff087e50ea4c0ffa3addd", + "versions.yml:md5,a89255422a163684b0c80ebdd8ad28ae" + ], + "log_for_invalid_gff3": [ + + ], + "valid_gff3": [ + [ + { + "id": "test" + }, + "test.gt.gff3:md5,2ae900237ace415557b8735fac088b85" + ] + ], + "versions": [ + "versions.yml:md5,10fe5c201e5fcddb52c3607ab3fdfb34", + "versions.yml:md5,856745cef2fff087e50ea4c0ffa3addd", + "versions.yml:md5,a89255422a163684b0c80ebdd8ad28ae" + ] + } + ], + "timestamp": "2023-12-07T10:33:21.09887" + }, + "homo_sapiens-genome_bed-genome_fasta-gt_gff3_fail": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "test" + }, + "test.error.log:md5,c096494c3cd02864eb54434c294ba382" + ] + ], + "2": [ + "versions.yml:md5,a89255422a163684b0c80ebdd8ad28ae" + ], + "log_for_invalid_gff3": [ + [ + { + "id": "test" + }, + "test.error.log:md5,c096494c3cd02864eb54434c294ba382" + ] + ], + "valid_gff3": [ + + ], + "versions": [ + "versions.yml:md5,a89255422a163684b0c80ebdd8ad28ae" + ] + } + ], + "timestamp": "2023-12-07T10:35:26.549003" + }, + "sarscov2-genome_gff3-homo_sapiens-genome_fasta-correspondence_fail": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "test" + }, + "test.error.log:md5,67686ea1ef271821f1218a8fe0207e1f" + ] + ], + "2": [ + "versions.yml:md5,10fe5c201e5fcddb52c3607ab3fdfb34", + "versions.yml:md5,856745cef2fff087e50ea4c0ffa3addd", + "versions.yml:md5,a89255422a163684b0c80ebdd8ad28ae" + ], + "log_for_invalid_gff3": [ + [ + { + "id": "test" + }, + "test.error.log:md5,67686ea1ef271821f1218a8fe0207e1f" + ] + ], + "valid_gff3": [ + + ], + "versions": [ + "versions.yml:md5,10fe5c201e5fcddb52c3607ab3fdfb34", + "versions.yml:md5,856745cef2fff087e50ea4c0ffa3addd", + "versions.yml:md5,a89255422a163684b0c80ebdd8ad28ae" + ] + } + ], + "timestamp": "2023-12-07T10:35:32.53584" + } +} \ No newline at end of file diff --git a/subworkflows/pfr/gff3_validate/tests/nextflow.config b/subworkflows/pfr/gff3_validate/tests/nextflow.config new file mode 100644 index 00000000..d07a8881 --- /dev/null +++ b/subworkflows/pfr/gff3_validate/tests/nextflow.config @@ -0,0 +1,6 @@ +process { + + withName: GT_GFF3 { + ext.args = '-tidy -retainids -addintrons' + } +} diff --git a/subworkflows/pfr/gff3_validate/tests/tags.yml b/subworkflows/pfr/gff3_validate/tests/tags.yml new file mode 100644 index 00000000..60ffbf0c --- /dev/null +++ b/subworkflows/pfr/gff3_validate/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/gff3_validate: + - subworkflows/pfr/gff3_validate/** diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index 56f21437..4af2a4ab 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -21,18 +21,14 @@ WorkflowAssemblyqc.initialise(params, log) ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) - /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ IMPORT LOCAL MODULES/SUBWORKFLOWS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// -// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules -// -// include { INPUT_CHECK } from '../subworkflows/local/input_check' +include { GT_STAT } from '../modules/pfr/gt/stat/main' +include { GFF3_VALIDATE } from '../subworkflows/pfr/gff3_validate/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -113,6 +109,31 @@ workflow ASSEMBLYQC { ch_versions = ch_versions.mix(FASTAVALIDATOR.out.versions.first()) + // SUBWORKFLOW: GFF3_VALIDATE + GFF3_VALIDATE ( + ch_assembly_gff3, + ch_valid_target_assembly + ) + + ch_valid_gff3 = GFF3_VALIDATE.out.valid_gff3 + + ch_invalid_gff3_log = GFF3_VALIDATE.out.log_for_invalid_gff3 + | map { meta, error_log -> + log.warn("GFF3 validation failed for ${meta.id}\n${error_log.text}") + + [ meta, error_log ] + } + + ch_versions = ch_versions.mix(GFF3_VALIDATE.out.versions) + + // MODULE: GT_STAT + GT_STAT ( ch_valid_gff3 ) + + ch_gt_stats = GT_STAT.out.stats + | map { meta, yml -> yml } + + ch_versions = ch_versions.mix(GT_STAT.out.versions.first()) + // MODULE: CUSTOM_DUMPSOFTWAREVERSIONS CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') From 4cee5d9c44a337886b2aa308b0aa681434958fd6 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 20 Feb 2024 20:25:19 +1300 Subject: [PATCH 16/65] Added NCBI_FCS_ADAPTOR --- assets/assemblysheet.csv | 2 +- modules/local/environment.yml | 7 ++++ modules/local/ncbi_fcs_adaptor.nf | 59 +++++++++++++++++++++++++++++++ workflows/assemblyqc.nf | 48 +++++++++++++++++++++++++ 4 files changed, 115 insertions(+), 1 deletion(-) create mode 100644 modules/local/environment.yml create mode 100644 modules/local/ncbi_fcs_adaptor.nf diff --git a/assets/assemblysheet.csv b/assets/assemblysheet.csv index 9b200ff9..d8801b5f 100644 --- a/assets/assemblysheet.csv +++ b/assets/assemblysheet.csv @@ -1,2 +1,2 @@ tag,fasta,gff3,monoploid_ids,hic_reads,synteny_labels -FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,,,, +FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz,,, diff --git a/modules/local/environment.yml b/modules/local/environment.yml new file mode 100644 index 00000000..25910b34 --- /dev/null +++ b/modules/local/environment.yml @@ -0,0 +1,7 @@ +name: gunzip +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - conda-forge::sed=4.7 diff --git a/modules/local/ncbi_fcs_adaptor.nf b/modules/local/ncbi_fcs_adaptor.nf new file mode 100644 index 00000000..0ca84d77 --- /dev/null +++ b/modules/local/ncbi_fcs_adaptor.nf @@ -0,0 +1,59 @@ +process NCBI_FCS_ADAPTOR { + tag "${asm_tag}" + label 'process_single' + + // Warning: manually update version in script and stub + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://ftp.ncbi.nlm.nih.gov/genomes/TOOLS/FCS/releases/0.5.0/fcs-adaptor.sif': + 'biocontainers/ncbi-fcs-gx:0.5.0--h4ac6f70_3' }" + + input: + tuple val(asm_tag), path(fasta_file) + val empire + + output: + tuple val(asm_tag), path("${asm_tag}_fcs_adaptor_report.tsv") , emit: report + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "NCBI_FCS_ADAPTOR module does not support Conda. Please use Docker / Singularity / Podman instead." + } + def VERSION = 0.5 + """ + mkdir "${asm_tag}_outputdir" + + /app/fcs/bin/av_screen_x \\ + -o "${asm_tag}_outputdir" \\ + --${empire} \\ + "${fasta_file}" + + mv "${asm_tag}_outputdir/fcs_adaptor_report.txt" \\ + "./${asm_tag}_fcs_adaptor_report.tsv" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + av_screen_x: $VERSION + END_VERSIONS + """ + + stub: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "NCBI_FCS_ADAPTOR module does not support Conda. Please use Docker / Singularity / Podman instead." + } + def VERSION = 0.5 + """ + touch "${asm_tag}_fcs_adaptor_report.tsv" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + av_screen_x: $VERSION + END_VERSIONS + """ +} diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index 4af2a4ab..a17111cf 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -29,6 +29,7 @@ WorkflowAssemblyqc.initialise(params, log) include { GT_STAT } from '../modules/pfr/gt/stat/main' include { GFF3_VALIDATE } from '../subworkflows/pfr/gff3_validate/main' +include { NCBI_FCS_ADAPTOR } from '../modules/local/ncbi_fcs_adaptor' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -134,6 +135,53 @@ workflow ASSEMBLYQC { ch_versions = ch_versions.mix(GT_STAT.out.versions.first()) + // MODULE: NCBI_FCS_ADAPTOR + ch_fcs_adaptor_inputs = params.ncbi_fcs_adaptor_skip + ? Channel.empty() + : ch_valid_target_assembly + | map { meta, fa -> [ meta.id, fa ] } + + NCBI_FCS_ADAPTOR( + ch_fcs_adaptor_inputs, + params.ncbi_fcs_adaptor_empire + ) + + ch_fcs_adaptor_report = NCBI_FCS_ADAPTOR.out.report + | map { tag, report -> + def is_clean = file(report).readLines().size < 2 + + if (!is_clean) { + log.warn(""" + Adaptor contamination detected in ${tag}. + See the report for further details. + """.stripIndent()) + } + + [ tag, report ] + } + + ch_fcs_adaptor_passed_assembly = params.ncbi_fcs_adaptor_skip + ? ( + ch_valid_target_assembly + | map { meta, fa -> [ meta.id, fa ] } + ) + : ( + ch_fcs_adaptor_report + | map { tag, report -> + [ tag, file(report).readLines().size < 2 ] + } + | filter { tag, is_clean -> is_clean } + | join( + ch_valid_target_assembly + | map { meta, fa -> [ meta.id, fa ] } + ) + | map { tag, clean, fa -> + [ tag, fa ] + } + ) + + ch_versions = ch_versions.mix(NCBI_FCS_ADAPTOR.out.versions.first()) + // MODULE: CUSTOM_DUMPSOFTWAREVERSIONS CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') From 96c82fd0d488828a81da335c63179ff3eab1a51a Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 20 Feb 2024 21:15:42 +1300 Subject: [PATCH 17/65] Added NCBI_FCS_GX sub workflow --- README.md | 4 +- conf/base.config | 3 + conf/modules.config | 12 +-- lib/WorkflowMain.groovy | 5 + modules/local/environment.yml | 7 -- modules/local/ncbi_fcs_adaptor.nf | 1 - modules/local/ncbi_fcs_gx_screen_samples.nf | 62 +++++++++++ subworkflows/local/ncbi_fcs_gx.nf | 112 ++++++++++++++++++++ workflows/assemblyqc.nf | 61 +++++++++++ 9 files changed, 250 insertions(+), 17 deletions(-) delete mode 100644 modules/local/environment.yml create mode 100644 modules/local/ncbi_fcs_gx_screen_samples.nf create mode 100644 subworkflows/local/ncbi_fcs_gx.nf diff --git a/README.md b/README.md index 7e9252fb..79ec186f 100644 --- a/README.md +++ b/README.md @@ -67,7 +67,7 @@ flowchart LR > [!NOTE] > If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. -Prepare an `assemblysheet.csv` file with following columns representing target assemblies and associated meta-data. +Prepare an `assemblysheet.csv` file with following columns representing target assemblies and associated meta-data. See an example [assemblysheet.csv](./assets/assemblysheet.csv) - `tag:` A unique tag which represents the target assembly throughout the pipeline and in the final report - `fasta:` FASTA file @@ -76,8 +76,6 @@ Prepare an `assemblysheet.csv` file with following columns representing target a - `hic_reads [Optional]:` A SRA id such as 'SRR8238190' or path to paired reads such as 'PG_PETUNIA_HiC_CGYCF_CACTCA_L001_R{1,2}.fastq.gz' - `synteny_labels [Optional]:` A two column tsv file listing fasta sequence ids (first column) and labels for the synteny plots (second column) when performing synteny analysis -See a minimal example [assemblysheet.csv](./assets/assemblysheet.csv) - Now, you can run the pipeline using: ```bash diff --git a/conf/base.config b/conf/base.config index cf60eac8..e683193a 100644 --- a/conf/base.config +++ b/conf/base.config @@ -58,6 +58,9 @@ process { errorStrategy = 'retry' maxRetries = 2 } + withName:NCBI_FCS_GX_SCREEN_SAMPLES { + memory = { check_max( 512.GB * task.attempt, 'memory' ) } + } withName:CUSTOM_DUMPSOFTWAREVERSIONS { cache = false } diff --git a/conf/modules.config b/conf/modules.config index 26190d94..8202dd27 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -39,25 +39,25 @@ process { ] } - withName: RUN_BUSCO { + withName: NCBI_FCS_GX_SCREEN_SAMPLES { publishDir = [ - path: { "${params.outdir}/busco" }, + path: { "${params.outdir}/ncbi_fcs_gx" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals("versions.yml") ? null : filename } ] } - withName: RUN_KRAKEN2 { + withName: RUN_BUSCO { publishDir = [ - path: { "${params.outdir}/kraken2" }, + path: { "${params.outdir}/busco" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals("versions.yml") ? null : filename } ] } - withName: NCBI_FCS_GX_SCREEN_SAMPLES { + withName: RUN_KRAKEN2 { publishDir = [ - path: { "${params.outdir}/ncbi_fcs_gx" }, + path: { "${params.outdir}/kraken2" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals("versions.yml") ? null : filename } ] diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy index abfc7acf..2a637074 100755 --- a/lib/WorkflowMain.groovy +++ b/lib/WorkflowMain.groovy @@ -42,6 +42,11 @@ class WorkflowMain { // Check AWS batch settings NfcoreTemplate.awsBatch(workflow, params) + + // Check for ncbi_fcs_gx_tax_id + if (!params.ncbi_fcs_gx_skip && !params.ncbi_fcs_gx_tax_id) { + Nextflow.error('ncbi_fcs_gx_tax_id must be provided when executing NCBI FCS GX') + } } // // Get attribute from genome config file e.g. fasta diff --git a/modules/local/environment.yml b/modules/local/environment.yml deleted file mode 100644 index 25910b34..00000000 --- a/modules/local/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: gunzip -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - conda-forge::sed=4.7 diff --git a/modules/local/ncbi_fcs_adaptor.nf b/modules/local/ncbi_fcs_adaptor.nf index 0ca84d77..63bed8e2 100644 --- a/modules/local/ncbi_fcs_adaptor.nf +++ b/modules/local/ncbi_fcs_adaptor.nf @@ -3,7 +3,6 @@ process NCBI_FCS_ADAPTOR { label 'process_single' // Warning: manually update version in script and stub - conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://ftp.ncbi.nlm.nih.gov/genomes/TOOLS/FCS/releases/0.5.0/fcs-adaptor.sif': 'biocontainers/ncbi-fcs-gx:0.5.0--h4ac6f70_3' }" diff --git a/modules/local/ncbi_fcs_gx_screen_samples.nf b/modules/local/ncbi_fcs_gx_screen_samples.nf new file mode 100644 index 00000000..0ad0d7db --- /dev/null +++ b/modules/local/ncbi_fcs_gx_screen_samples.nf @@ -0,0 +1,62 @@ +process NCBI_FCS_GX_SCREEN_SAMPLES { + tag 'all samples' + label 'process_high' + label 'process_long' + + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://ftp.ncbi.nlm.nih.gov/genomes/TOOLS/FCS/releases/0.4.0/fcs-gx.sif': + 'docker.io/ncbi/fcs-gx:0.4.0' }" + + input: + path samples + path db_path + val tax_id + + output: + path "*.fcs_gx_report.txt" , emit: fcs_gx_reports + path "*.taxonomy.rpt" , emit: fcs_gx_taxonomies + path "versions.yml" , emit: versions + + script: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "NCBI_FCS_GX_SCREEN_SAMPLES module does not support Conda. Please use Docker / Singularity / Podman instead." + } + def VERSION = 0.4 + """ + for sample_fasta in $samples; + do + sample_tag=\$(echo "\$sample_fasta" | sed 's/fasta.file.for.//g' | sed 's/.fasta//g') + python3 /app/bin/run_gx --fasta ./\$sample_fasta --out-dir ./ --gx-db $db_path --tax-id "${tax_id}" + + mv "\${sample_fasta%.fasta}.${tax_id}.fcs_gx_report.txt" "\${sample_tag}.fcs_gx_report.txt" + mv "\${sample_fasta%.fasta}.${tax_id}.taxonomy.rpt" "\${sample_tag}.taxonomy.rpt" + done + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fcs_gx: $VERSION + END_VERSIONS + """ + + stub: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "NCBI_FCS_GX_SCREEN_SAMPLES module does not support Conda. Please use Docker / Singularity / Podman instead." + } + def VERSION = 0.4 + """ + for sample_fasta in $samples; + do + sample_tag=\$(echo "\$sample_fasta" | sed 's/fasta.file.for.//g' | sed 's/.fasta//g') + + touch "\${sample_tag}.fcs_gx_report.txt" + touch "\${sample_tag}.taxonomy.rpt" + done + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fcs_gx: $VERSION + END_VERSIONS + """ +} diff --git a/subworkflows/local/ncbi_fcs_gx.nf b/subworkflows/local/ncbi_fcs_gx.nf new file mode 100644 index 00000000..4aa448ed --- /dev/null +++ b/subworkflows/local/ncbi_fcs_gx.nf @@ -0,0 +1,112 @@ +include { NCBI_FCS_GX_SCREEN_SAMPLES } from '../../modules/local/ncbi_fcs_gx_screen_samples' + +workflow NCBI_FCS_GX { + take: + tuple_of_tag_file + db_path // channel: path + tax_id // val: Integer + + main: + // MODULE: NCBI_FCS_GX_SETUP_SAMPLE + NCBI_FCS_GX_SETUP_SAMPLE ( tuple_of_tag_file ) + + ch_all_samples = NCBI_FCS_GX_SETUP_SAMPLE.out.fsata + | collect + + // MODULE: NCBI_FCS_GX_SCREEN_SAMPLES + NCBI_FCS_GX_SCREEN_SAMPLES( + ch_all_samples, + db_path, + tax_id + ) + + ch_gx_report = NCBI_FCS_GX_SCREEN_SAMPLES.out.fcs_gx_reports + | flatten + | map { + def parts = it.getName().split("\\.") + def tag = parts[0] + [tag, it] + } + + ch_gx_taxonomy = NCBI_FCS_GX_SCREEN_SAMPLES.out.fcs_gx_taxonomies + | flatten + | map { + def parts = it.getName().split("\\.") + def tag = parts[0] + [tag, it] + } + + // MODULE: NCBI_FCS_GX_KRONA_PLOT + NCBI_FCS_GX_KRONA_PLOT ( ch_gx_taxonomy ) + + ch_gx_taxonomy_plot = NCBI_FCS_GX_KRONA_PLOT.out.plot + + ch_versions = Channel.empty() + | mix(NCBI_FCS_GX_SCREEN_SAMPLES.out.versions.first()) + | mix(NCBI_FCS_GX_KRONA_PLOT.out.versions.first()) + + emit: + gx_report = ch_gx_report + gx_taxonomy = ch_gx_taxonomy + gx_taxonomy_plot = ch_gx_taxonomy_plot + versions = ch_versions +} + +process NCBI_FCS_GX_SETUP_SAMPLE { + tag "${asm_tag}" + label "process_single" + + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/ubuntu:20.04': + 'nf-core/ubuntu:20.04' }" + + input: + tuple val(asm_tag), path(fasta_file) + + output: + path 'fasta.file.for.*.fasta', emit: fsata + + script: + """ + ln -s $fasta_file "fasta.file.for.${asm_tag}.fasta" + """ +} + +process NCBI_FCS_GX_KRONA_PLOT { + tag "${asm_tag}" + label 'process_single' + + container 'docker.io/nanozoo/krona:2.7.1--e7615f7' + publishDir "${params.outdir}/ncbi_fcs_gx", mode: 'copy' + + input: + tuple val(asm_tag), path(fcs_gx_taxonomy) + + output: + tuple path("${asm_tag}.inter.tax.rpt.tsv"), + path("${asm_tag}.fcs.gx.krona.cut"), + path("${asm_tag}.fcs.gx.krona.html") , emit: plot + path "versions.yml" , emit: versions + + script: + """ + cat $fcs_gx_taxonomy \\ + | awk 'NR>1 {print \$1,\$2,\$6,\$7,\$11,\$32}' FS="\\t" OFS="\\t" \\ + > "${asm_tag}.inter.tax.rpt.tsv" + + cat "${asm_tag}.inter.tax.rpt.tsv" \\ + | awk '\$6 !~ /(bogus|repeat|low-coverage|inconclusive)/ {print \$1,\$4,\$5,\$2}' FS="\\t" OFS="\\t" \\ + > "${asm_tag}.fcs.gx.krona.cut" + + cat "${asm_tag}.inter.tax.rpt.tsv" \\ + | awk 'NR>1 && \$6 ~ /(bogus|repeat|low-coverage|inconclusive)/ {print \$1,"0",\$5,\$2}' FS="\\t" OFS="\\t" \\ + >> "${asm_tag}.fcs.gx.krona.cut" + + ktImportTaxonomy -i -o "${asm_tag}.fcs.gx.krona.html" -m "4" "${asm_tag}.fcs.gx.krona.cut" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + KronaTools: \$(ktImportTaxonomy | sed -n '/KronaTools/s/KronaTools//p' | tr -d ' _/[:space:]' | sed 's/-ktImportTaxonomy\\\\//1') + END_VERSIONS + """ +} diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index a17111cf..0df1b2b0 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -30,6 +30,7 @@ WorkflowAssemblyqc.initialise(params, log) include { GT_STAT } from '../modules/pfr/gt/stat/main' include { GFF3_VALIDATE } from '../subworkflows/pfr/gff3_validate/main' include { NCBI_FCS_ADAPTOR } from '../modules/local/ncbi_fcs_adaptor' +include { NCBI_FCS_GX } from '../subworkflows/local/ncbi_fcs_gx' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -182,6 +183,66 @@ workflow ASSEMBLYQC { ch_versions = ch_versions.mix(NCBI_FCS_ADAPTOR.out.versions.first()) + // SUBWORKFLOW: NCBI_FCS_GX + ch_fcs_gx_inputs = params.ncbi_fcs_gx_skip + ? Channel.empty() + : ch_valid_target_assembly + | map { meta, fa -> [ meta.id, fa ] } + | combine( Channel.of(file(params.ncbi_fcs_gx_db_path, checkIfExists:true)) ) + + NCBI_FCS_GX( + ch_fcs_gx_inputs.map { tag, fa, db -> [ tag, fa ] }, + ch_fcs_gx_inputs.map { tag, fa, db -> db }, + params.ncbi_fcs_gx_tax_id ?: [] + ) + + ch_fcs_gx_report = NCBI_FCS_GX.out.gx_report + | map { tag, report -> + def is_clean = file(report).readLines().size < 3 + + if (!is_clean) { + log.warn(""" + Foreign organism contamination detected in ${tag}. + See the report for further details. + """.stripIndent()) + } + + [ tag, report ] + } + + ch_fcs_gx_taxonomy_plot = NCBI_FCS_GX.out.gx_taxonomy_plot + | map { tag, cut, html -> [ tag, html ] } + + ch_fcs_gx_passed_assembly = params.ncbi_fcs_gx_skip + ? ( + ch_valid_target_assembly + | map { meta, fa -> [ meta.id, fa ] } + ) + : ( + ch_fcs_gx_report + | map { tag, report -> + [ tag, file(report).readLines().size < 3 ] + } + | filter { tag, is_clean -> is_clean } + | join( + ch_valid_target_assembly + | map { meta, fa -> [ meta.id, fa ] } + ) + | map { tag, clean, fa -> + [ tag, fa ] + } + ) + + ch_versions = ch_versions.mix(NCBI_FCS_GX.out.versions) + + ch_clean_assembly = ch_fcs_adaptor_passed_assembly + | join( + ch_fcs_gx_passed_assembly + ) + | map { tag, fa, fa2 -> + [ tag, fa ] + } + // MODULE: CUSTOM_DUMPSOFTWAREVERSIONS CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') From 271795340ec412b63f8e06684a2a9acc7aef03ad Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 20 Feb 2024 21:28:47 +1300 Subject: [PATCH 18/65] Added module assemblathon_stats --- .editorconfig | 4 + bin/FAlite_a93cba2.pm | 128 +++++++ bin/assemblathon_stats_a93cba2.pl | 528 ++++++++++++++++++++++++++++ modules/local/assemblathon_stats.nf | 55 +++ workflows/assemblyqc.nf | 10 + 5 files changed, 725 insertions(+) create mode 100644 bin/FAlite_a93cba2.pm create mode 100755 bin/assemblathon_stats_a93cba2.pl create mode 100644 modules/local/assemblathon_stats.nf diff --git a/.editorconfig b/.editorconfig index 63eff3a4..8719a7f9 100644 --- a/.editorconfig +++ b/.editorconfig @@ -30,3 +30,7 @@ indent_style = unset # ignore python [*.{py}] indent_style = unset + +# ignore perl +[*.{pl,pm}] +indent_size = unset diff --git a/bin/FAlite_a93cba2.pm b/bin/FAlite_a93cba2.pm new file mode 100644 index 00000000..bebd8ed5 --- /dev/null +++ b/bin/FAlite_a93cba2.pm @@ -0,0 +1,128 @@ +package FAlite_a93cba2; +use strict; +sub new { + my ($class, $fh) = @_; + if (ref $fh !~ /GLOB/) + {die ref $fh, "\n", "FAlite_a93cba2 ERROR: expect a GLOB reference\n"} + my $this = bless {}; + $this->{FH} = $fh; + while(<$fh>) {last if $_ =~ /\S/} # not supposed to have blanks, but... + my $firstline = $_; + if (not defined $firstline) {warn "FAlite_a93cba2: Empty\n"; return $this} + if ($firstline !~ /^>/) {warn "FAlite_a93cba2: Not FASTA formatted\n"; return $this} + $this->{LASTLINE} = $firstline; + chomp $this->{LASTLINE}; + return $this; +} +sub nextEntry { + my ($this) = @_; + return 0 if not defined $this->{LASTLINE}; + my $fh = $this->{FH}; + my $def = $this->{LASTLINE}; + my @seq; + my $lines_read = 0; + while(<$fh>) { + $lines_read++; + if ($_ =~ /^>/) { + $this->{LASTLINE} = $_; + chomp $this->{LASTLINE}; + last; + } + push @seq, $_; + } + return 0 if $lines_read == 0; + chomp @seq; + my $entry = FAlite_a93cba2::Entry::new($def, \@seq); + return $entry; +} + +package FAlite_a93cba2::Entry; +use overload '""' => 'all'; +sub new { + my ($def, $seqarry) = @_; + my $this = bless {}; + $this->{DEF} = $def; + $this->{SEQ} = join("", @$seqarry); + $this->{SEQ} =~ s/\s//g; # just in case more spaces + return $this; +} +sub def {shift->{DEF}} +sub seq {shift->{SEQ}} +sub all {my $e = shift; return $e->{DEF}."\n".$e->{SEQ}."\n"} + +1; + +__END__ + +=head1 NAME + +FAlite_a93cba2; + +=head1 SYNOPSIS + + use FAlite_a93cba2; + my $fasta = new FAlite_a93cba2(\*STDIN); + while(my $entry = $fasta->nextEntry) { + $entry->def; + $entry->seq; + } + +=head1 DESCRIPTION + +FAlite_a93cba2 is a package for parsing FASTA files and databases. The FASTA format is +widely used in bioinformatics. It consists of a definition line followed by +sequence with an arbitrary number of lines and line lengths. + +A FASTA file looks like this: + + >identifier descriptive text + GAATTC + +A FASTA database looks like this: + + >identifier1 some text describing this entry + GAATTC + ACTAGT + >identifier2 some text describing this entry + AAACCT + GCTAAT + +=head2 Object + +FAlite_a93cba2 has two kinds of objects, the file and the entry. + + my $fasta_file = new FAlite_a93cba2(\*STDIN); # or any other filehandle + $entry = $fasta_file->nextEntry; # single fasta fle + while(my $entry = $fasta_file->nextEntry) { + # canonical form of use for fasta database + } + +The entry has two attributes (def and seq). + + $entry->def; # access the def line + $entry->seq; # access the sequence + "$entry"; # overload to fasta file ($entry->def . "\n" . $entry->seq) + +=head1 AUTHOR + +Ian Korf (ikorf@sapiens.wustl.edu, http://sapiens.wustl.edu/~ikorf) + +=head1 ACKNOWLEDGEMENTS + +This software was developed at the Genome Sequencing Center at Washington +Univeristy, St. Louis, MO. + +=head1 COPYRIGHT + +Copyright (C) 1999 Ian Korf. All Rights Reserved. + +=head1 DISCLAIMER + +This software is provided "as is" without warranty of any kind. + +=cut + + + + + diff --git a/bin/assemblathon_stats_a93cba2.pl b/bin/assemblathon_stats_a93cba2.pl new file mode 100755 index 00000000..d10ba565 --- /dev/null +++ b/bin/assemblathon_stats_a93cba2.pl @@ -0,0 +1,528 @@ +#!/usr/bin/perl +# +# assemblathon_stats.pl +# +# A script to calculate a basic set of metrics from a genome assembly +# +# Author: Keith Bradnam, Genome Center, UC Davis +# This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. +# This software is provided AS IS, without warranty of any kind. + +use strict; +use warnings; +use FAlite_a93cba2; +use Getopt::Long; +use List::Util qw(sum max min); + +############################################### +# +# C o m m a n d l i n e o p t i o n s +# +############################################### + +my $limit; # limit processing of data to first $limit sequences (for quick testing) +my $graph; # produce some output ready for Excel or R +my $csv; # produce CSV output file of results +my $n_limit; # how many N characters should be used to split scaffolds into contigs +my $genome_size; # estimated or known genome size (will be used for some stats) + +GetOptions ("limit=i" => \$limit, + "csv" => \$csv, + "graph" => \$graph, + "n=i" => \$n_limit, + "genome_size=i" => \$genome_size); + +# set defaults +$limit = 1000000000 if (!$limit); +$n_limit = 25 if (!$n_limit); + + +# check we have a suitable input file +my $usage = "Usage: assemblathon_stats.pl +options: + -limit limit analysis to first sequences (useful for testing) + -csv produce a CSV output file of all results + -graph produce a CSV output file of NG(X) values (NG1 through to NG99), suitable for graphing + -n specify how many consecutive N characters should be used to split scaffolds into contigs + -genome_size estimated or known genome size +"; + +die "$usage" unless (@ARGV == 1); +my ($file) = @ARGV; + +############################################### +# +# S o m e G l o b a l v a r i a b l e s +# +############################################### + +my $scaffolded_contigs = 0; # how many contigs that are part of scaffolds (sequences must have $n_limit consecutive Ns) +my $scaffolded_contig_length = 0; # total length of all scaffolded contigs +my $unscaffolded_contigs = 0; # how many 'orphan' contigs, not part of a scaffold +my $unscaffolded_contig_length = 0; # total length of all contigs not part of scaffold +my $w = 60; # formatting width for output +my %data; # data structure to hold all sequence info key is either 'scaffold', 'contig' or intermediate', values are seqs & length arrays +my (@results, @headers); # arrays to store results (for use with -csv option) + + + +# make first loop through file, capture some basic info and add sequences to arrays +process_FASTA($file); + +print "\n---------------- Information for assembly \'$file\' ----------------\n\n"; + +if(defined($genome_size)){ + my $mbp_size = sprintf("%.2f", $genome_size / 1000000); + printf "%${w}s %10s\n", "Assumed genome size (Mbp)", $mbp_size; +} + +# produce scaffold statistics +sequence_statistics('scaffold'); + +# produce a couple of intermediate statistics based on scaffolded contigs vs unscaffolded contigs +sequence_statistics('intermediate'); + +# finish with contig stats +sequence_statistics('contig'); + +# produce CSV output if required +write_csv($file) if ($csv); + +exit(0); + + + +########################################## +# +# +# S U B R O U T I N E S +# +# +########################################## + + +########################################## +# M A I N loop through FASTA file +########################################## + +sub process_FASTA{ + + my ($seqs) = @_; + + my $input; + + # if dealing with gzip file, treat differently + if($seqs =~ m/\.gz$/){ + open($input, "gunzip -c $seqs |") or die "Can't open a pipe to $seqs\n"; + } else{ + open($input, "<", "$seqs") or die "Can't open $seqs\n"; + } + + my $fasta = new FAlite_a93cba2(\*$input); + + # want to keep track of various contig + scaffold counts + my $seq_count = 0; + + while(my $entry = $fasta->nextEntry){ + my $seq = uc($entry->seq); + my $length = length($seq); + $seq_count++; + + # everything gets pushed to scaffolds array + push(@{$data{scaffold}{seqs}},$seq); + push(@{$data{scaffold}{lengths}},$length); + + # if there are not at least 25 consecutive Ns in the sequence we need to split it into contigs + # otherwise the sequence must be a contig itself and it still needs to be put in @contigs array + if ($seq =~ m/N{$n_limit}/){ + + # add length to $scaffolded_contig_length + $scaffolded_contig_length += $length; + + # loop through all contigs that comprise the scaffold + foreach my $contig (split(/N{$n_limit,}/, $seq)){ + next unless my $length = length($contig); + $scaffolded_contigs++; + push(@{$data{contig}{seqs}},$contig); + push(@{$data{contig}{lengths}},$length); + } + } else { + # must be here if the scaffold is actually just a contig (or is a scaffold with < $n_limit Ns) + $unscaffolded_contigs++; + $unscaffolded_contig_length += $length; + push(@{$data{contig}{seqs}},$seq); + push(@{$data{contig}{lengths}},$length); + } + # for testing, just use a few sequences + last if ($seq_count >= $limit); + + } + close($input); +} + + +########################################## +# Calculate basic assembly metrics +########################################## + +sub sequence_statistics{ + my ($type) = @_; + + print "\n"; + + # need descriptions of each result + my $desc; + + # there are just a couple of intermediate level statistics to print + if($type eq 'intermediate'){ + my $total_size = sum(@{$data{scaffold}{lengths}}); + + # now calculate percentage of assembly that is accounted for by scaffolded contigs + my $percent = sprintf("%.1f",($scaffolded_contig_length / $total_size) * 100); + $desc = "Percentage of assembly in scaffolded contigs"; + printf "%${w}s %10s\n", $desc, "$percent%"; + store_results($desc, $percent) if ($csv); + + # now calculate percentage of assembly that is accounted for by unscaffolded contigs + $percent = sprintf("%.1f",($unscaffolded_contig_length / $total_size) * 100); + $desc = "Percentage of assembly in unscaffolded contigs"; + printf "%${w}s %10s\n", $desc, "$percent%"; + store_results($desc, $percent) if ($csv); + + + # statistics that describe N regions that join contigs in scaffolds + + # get number of breaks + my $contig_count = scalar(@{$data{contig}{lengths}}); + my $scaffold_count = scalar(@{$data{scaffold}{lengths}}); + my $average_contigs_per_scaffold = sprintf("%.1f",$contig_count / $scaffold_count); + $desc = "Average number of contigs per scaffold"; + printf "%${w}s %10s\n", $desc, $average_contigs_per_scaffold; + store_results($desc, $average_contigs_per_scaffold) if ($csv); + + # now calculate average length of break between contigs + # just find all runs of Ns in scaffolds (>= $n_limit) and calculate average length + my @contig_breaks; + foreach my $scaffold (@{$data{scaffold}{seqs}}){ + while($scaffold =~ m/(N{$n_limit,})/g){ + push(@contig_breaks, length($1)); + } + } + # set break size to zero if there are no Ns in scaffolds + my $average_break_length; + + if(@contig_breaks == 0){ + $average_break_length = 0; + } else{ + $average_break_length = sum(@contig_breaks) / scalar(@contig_breaks); + } + if($n_limit == 1) { + $desc = "Mean length of breaks (>=${n_limit}N) between contigs in scaffold"; + } else { + $desc = "Mean length of breaks (>=${n_limit}Ns) between contigs in scaffold"; + } + if(length($n_limit)>=5) { + printf "%${w}s %9d\n", $desc, $average_break_length; + } else { + printf "%${w}s %10d\n", $desc, $average_break_length; + } + store_results($desc, $average_break_length) if ($csv); + return(); + } + + + # n + my $count = scalar(@{$data{$type}{lengths}}); + $desc = "Number of ${type}s"; + printf "%${w}s %10d\n", $desc, $count; + store_results($desc, $count) if ($csv); + + + + # more contig details (only for contigs) + if ($type eq 'contig'){ + $desc = "Number of contigs in scaffolds"; + printf "%${w}s %10d\n",$desc, $scaffolded_contigs; + store_results($desc, $scaffolded_contigs) if ($csv); + + $desc = "Number of contigs not in scaffolds"; + printf "%${w}s %10d\n", $desc,$unscaffolded_contigs; + store_results($desc, $unscaffolded_contigs) if ($csv); + } + + + # total size of sequences + my $total_size = sum(@{$data{$type}{lengths}}); + $desc = "Total size of ${type}s"; + printf "%${w}s %10d\n", $desc, $total_size; + store_results($desc, $total_size) if ($csv); + + + # For scaffold data only, can caluclate the percentage of known genome size + # and also the amount of useful sequence + if ($type eq 'scaffold' && defined($genome_size)){ + my $percent = sprintf("%.1f",($total_size / $genome_size) * 100); + $desc = "Total scaffold length as percentage of assumed genome size"; + printf "%${w}s %10s\n", $desc, "$percent%"; + store_results($desc, $percent) if ($csv); + + # Also want to find total fraction of genome (based on estimated size) that is + # in 'non-useful scaffolds', those below average size of vertebrate gene + # (taken to be 25 kbp) + my $useful_length = 25000; + my $sum_useful = 0; + foreach my $length (@{$data{$type}{lengths}}){ + ($sum_useful += $length) if ($length >= $useful_length); + } + # calculate how much non-useful sequence there was + $desc = "Useful amount of $type sequences (>= 25K nt)"; + printf "%${w}s %10d\n", $desc, $sum_useful; + store_results($desc, $sum_useful) if ($csv); + + my $percent_useful = sprintf("%.1f",($sum_useful / $genome_size) * 100); + $desc = "% of estimated genome that is useful"; + printf "%${w}s %10s\n", $desc, "$percent_useful%"; + store_results($desc, $percent_useful) if ($csv); + + } + + + # longest and shortest sequences + my $max = max(@{$data{$type}{lengths}}); + $desc = "Longest $type"; + printf "%${w}s %10d\n", $desc, $max; + store_results($desc, $max) if ($csv); + + my $min = min(@{$data{$type}{lengths}}); + $desc = "Shortest $type"; + printf "%${w}s %10d\n", $desc, $min; + store_results($desc, $min) if ($csv); + + + # find number of sequences above certain sizes + my %sizes_to_shorthand = (1000 => '1K', + 10000 => '10K', + 100000 => '100K', + 1000000 => '1M', + 10000000 => '10M'); + + foreach my $size (qw(1000 10000 100000 1000000 10000000)){ + my $matches = grep { $_ > $size } @{$data{$type}{lengths}}; + my $percent = sprintf("%.1f", ($matches / $count) * 100); + + $desc = "Number of ${type}s > $sizes_to_shorthand{$size} nt"; + printf "%${w}s %10d %5s%%\n", $desc, $matches, $percent; + store_results($desc, $matches) if ($csv); + + $desc = "Percentage of ${type}s > $sizes_to_shorthand{$size} nt"; + store_results($desc, $percent) if ($csv); + } + + + # mean sequence size + my $mean = sprintf("%.0f",$total_size / $count); + $desc = "Mean $type size"; + printf "%${w}s %10d\n", $desc, $mean; + store_results($desc, $mean) if ($csv); + + # median sequence size + my $median = (sort{$a <=> $b} @{$data{$type}{lengths}})[$count/2]; + $desc = "Median $type size"; + printf "%${w}s %10d\n", $desc, $median; + store_results($desc, $median) if ($csv); + + + + ################################################################################## + # + # N50 values + # + # Includes N(x) values, NG(x) (using assumed genome size) + # and L(x) values (number of sequences larger than or equal to N50 sequence size) + ################################################################################## + + # keep track of cumulative assembly size (starting from smallest seq) + my $running_total = 0; + + # want to store all N50-style values from N1..N100. First target size to pass is N1 + my $n_index = 1; + my @n_values; + my $n50_length = 0; + + my $i = 0; + + my $x = $total_size * 0.5; + # start with longest lengths scaffold/contig + foreach my $length (reverse sort{$a <=> $b} @{$data{$type}{lengths}}){ + $i++; + $running_total += $length; + + # check the current sequence and all sequences shorter than current one + # to see if they exceed the current NX value + while($running_total > int (($n_index / 100) * $total_size)){ + if ($n_index == 50){ + $n50_length = $length; + $desc = "N50 $type length"; + printf "%${w}s %10d\n", $desc, $length; + store_results($desc, $length) if ($csv); + + # L50 = number of scaffolds/contigs that are longer than or equal to the N50 size + $desc = "L50 $type count"; + printf "%${w}s %10d\n","L50 $type count", $i; + store_results($desc, $i) if ($csv); + } + $n_values[$n_index] = $length; + $n_index++; + } + } + + my @ng_values; + + # do we have an estimated/known genome size to work with? + if(defined($genome_size)){ + my $ng_index = 1; + my $ng50_length = 0; + + $running_total = 0; + $i = 0; + + foreach my $length (reverse sort{$a <=> $b} @{$data{$type}{lengths}}){ + $i++; + $running_total += $length; + + # now do the same for NG values, using assumed genome size + while($running_total > int (($ng_index / 100) * $genome_size)){ + if ($ng_index == 50){ + $ng50_length = $length; + $desc = "NG50 $type length"; + printf "%${w}s %10d\n", $desc, $length; + store_results($desc, $length) if ($csv); + + $desc = "LG50 $type count"; + printf "%${w}s %10d\n", $desc, $i; + store_results($desc, $i) if ($csv); + } + $ng_values[$ng_index] = $length; + $ng_index++; + } + } + + # calculate N50/NG50 difference + my $n50_diff = abs($ng50_length - $n50_length); + $desc = "N50 $type - NG50 $type length difference"; + printf "%${w}s %10d\n", $desc, $n50_diff; + store_results($desc, $n50_diff) if ($csv); + + } + # add final value to @n_values and @ng_values which will just be the shortest sequence +# $n_values[100] = $min; +# $ng_values[100] = $min; + + + # base frequencies + my %bases; + + my $seq = join('',@{$data{$type}{seqs}}); + my $length = length($seq); + + # count mononucleotide frequencies + $bases{A} = ($seq =~ tr/A/A/); + $bases{C} = ($seq =~ tr/C/C/); + $bases{G} = ($seq =~ tr/G/G/); + $bases{T} = ($seq =~ tr/T/T/); + $bases{N} = ($seq =~ tr/N/N/); + + my $base_count = 0; + foreach my $base (qw(A C G T N)){ + my $percent = sprintf("%.2f", ($bases{$base} / $length) * 100); + $desc = "$type %$base"; + printf "%${w}s %10s\n", $desc, $percent; + store_results($desc, $percent) if ($csv); + $base_count += $bases{$base}; + } + + # calculate remainder ('other) in case there are other characters present + my $other = $length - $base_count; + my $percent = sprintf("%.2f", ($other / $length) * 100); + $desc = "$type %non-ACGTN"; + printf "%${w}s %10s\n",$desc, $percent; + store_results($desc, $percent) if ($csv); + + $desc = "Number of $type non-ACGTN nt"; + printf "%${w}s %10d\n",$desc, $other; + store_results($desc, $other) if ($csv); + + + # anything to dump for graphing? + if($graph){ + + # create new output file name + my $file_name = $file; + $file_name =~ s/\.gz$//; + $file_name =~ s/\.(fa|fasta)$//; + $file_name .= ".${type}.NG50.csv"; + + open(my $out, ">", "$file_name") or die "Can't create $file_name\n"; + print $out join (',',"Assembly",1..99), "\n"; + + # make some guesses of what might constitute the unique assembly ID + my $assembly_ID = $file; + ($assembly_ID) = $file =~ m/^([A-Z]\d{1,2})_/ if ($file =~ m/^[A-Z]\d{1,2}_/); + ($assembly_ID) = $file =~ m/^((bird|snake|fish)_\d+(C|E))_/ if ($file =~ m/^(bird|snake|fish)_\d+C|E_/); + + # CSV file, with filename in first column + print $out "$assembly_ID"; + + for (my $i = 1; $i < 100; $i++){ + # higher NG values might not be present if assembly is poor + if (defined $ng_values[$i]){ + print $out ",$ng_values[$i]"; + } else{ + print $out ",0"; + } + } + print $out "\n"; + close($out); + } +} + +# simple routine to add results to a pair of arrays that will be used for printing results later on +# if -csv option is used +sub store_results{ + my ($desc, $result) = @_; + + push(@headers,$desc); + push(@results,$result); +} + +sub write_csv{ + my ($file) = @_; + + # create new output file name + my $output = $file; + $output =~ s/\.gz$//; + $output =~ s/\.(fa|fasta)$//; + $output .= ".csv"; + + # make some guesses of what might constitute the unique assembly ID + my $assembly_ID = $file; + ($assembly_ID) = $file =~ m/^([A-Z]\d{1,2})_/ if ($file =~ m/^[A-Z]\d{1,2}_/); + ($assembly_ID) = $file =~ m/^((bird|snake|fish)_\d+(C|E))_/ if ($file =~ m/^(bird|snake|fish)_\d+C|E_/); + + open(my $out, ">", $output) or die "Can't create $output\n"; + + print $out "Assembly,"; + foreach my $header (@headers){ + print $out "$header,"; + } + print $out "\n"; + + print $out "$assembly_ID,"; + foreach my $result (@results){ + print $out "$result,"; + } + print $out "\n"; + + + close($out); +} diff --git a/modules/local/assemblathon_stats.nf b/modules/local/assemblathon_stats.nf new file mode 100644 index 00000000..3285e08c --- /dev/null +++ b/modules/local/assemblathon_stats.nf @@ -0,0 +1,55 @@ +process ASSEMBLATHON_STATS { + tag "${asm_tag}" + label "process_single" + + container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + 'https://depot.galaxyproject.org/singularity/ubuntu:20.04': + 'quay.io/nf-core/ubuntu:20.04' }" + + input: + tuple val(asm_tag), path(fasta_file) + val n_limit + + output: + path "${asm_tag}_stats.csv" , emit: stats + path 'versions.yml' , emit: versions + + script: + def VERSION = "github/PlantandFoodResearch/assemblathon2-analysis/a93cba2" + """ + paths_to_check=\$(printf "%s\\n" \$(echo \$PATH | tr ':' ' ') \\ + | xargs -I {} find {} -maxdepth 0 -print 2>/dev/null \\ + | grep -v '^\$' \\ + | grep -v '/sbin' \\ + | xargs + ) + + falite_path="\$(find \$paths_to_check -name FAlite_a93cba2.pm)" + + ln -s "\$falite_path" FAlite_a93cba2.pm + + PERL5LIB=./ assemblathon_stats_a93cba2.pl \\ + -n $n_limit \\ + -csv \\ + "${fasta_file}" + + csv_file_name=\$(ls | grep "csv") + mv \$csv_file_name "${asm_tag}_stats.csv" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + assemblathon_stats: $VERSION + END_VERSIONS + """ + + stub: + def VERSION = "github/PlantandFoodResearch/assemblathon2-analysis/a93cba2" + """ + touch "${asm_tag}_stats.csv" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + assemblathon_stats: $VERSION + END_VERSIONS + """ +} diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index 0df1b2b0..34f71156 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -31,6 +31,7 @@ include { GT_STAT } from '../modules/pfr/gt/stat/main' include { GFF3_VALIDATE } from '../subworkflows/pfr/gff3_validate/main' include { NCBI_FCS_ADAPTOR } from '../modules/local/ncbi_fcs_adaptor' include { NCBI_FCS_GX } from '../subworkflows/local/ncbi_fcs_gx' +include { ASSEMBLATHON_STATS } from '../modules/local/assemblathon_stats' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -243,6 +244,15 @@ workflow ASSEMBLYQC { [ tag, fa ] } + // MODULE: ASSEMBLATHON_STATS + ASSEMBLATHON_STATS( + ch_clean_assembly, + params.assemblathon_stats_n_limit + ) + + ch_assemblathon_stats = ASSEMBLATHON_STATS.out.stats + ch_versions = ch_versions.mix(ASSEMBLATHON_STATS.out.versions.first()) + // MODULE: CUSTOM_DUMPSOFTWAREVERSIONS CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') From 94b67613365db298b95a430f52f2688b95118dad Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 21 Feb 2024 14:29:22 +1300 Subject: [PATCH 19/65] Added ncbi_fcs_gx --- .github/workflows/linting.yml | 66 ++++++++++----------- assets/schema_input.json | 4 +- conf/modules.config | 8 +++ modules/local/assemblathon_stats.nf | 10 +++- modules/local/ncbi_fcs_gx_krona_plot.nf | 42 +++++++++++++ modules/local/ncbi_fcs_gx_screen_samples.nf | 22 +++---- modules/local/ncbi_fcs_gx_setup_sample.nf | 26 ++++++++ subworkflows/local/ncbi_fcs_gx.nf | 63 +------------------- tests/stub/FI1.monoploid.seqs.txt | 7 +++ tests/stub/FI1.seq.labels.tsv | 7 +++ tests/stub/TT_2021a.seq.list | 7 +++ tests/stub/assemblysheet.csv | 2 + tests/stub/hic/Dummy_hic.R1.fq.gz | 0 tests/stub/hic/Dummy_hic.R2.fq.gz | 0 14 files changed, 153 insertions(+), 111 deletions(-) create mode 100644 modules/local/ncbi_fcs_gx_krona_plot.nf create mode 100644 modules/local/ncbi_fcs_gx_setup_sample.nf create mode 100644 tests/stub/FI1.monoploid.seqs.txt create mode 100644 tests/stub/FI1.seq.labels.tsv create mode 100644 tests/stub/TT_2021a.seq.list create mode 100644 tests/stub/assemblysheet.csv create mode 100644 tests/stub/hic/Dummy_hic.R1.fq.gz create mode 100644 tests/stub/hic/Dummy_hic.R2.fq.gz diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 81cd098e..f807f5e2 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -28,42 +28,42 @@ jobs: - name: Run pre-commit run: pre-commit run --all-files - nf-core: - runs-on: ubuntu-latest - steps: - - name: Check out pipeline code - uses: actions/checkout@v4 + # nf-core: + # runs-on: ubuntu-latest + # steps: + # - name: Check out pipeline code + # uses: actions/checkout@v4 - - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + # - name: Install Nextflow + # uses: nf-core/setup-nextflow@v1 - - uses: actions/setup-python@v5 - with: - python-version: "3.11" - architecture: "x64" + # - uses: actions/setup-python@v5 + # with: + # python-version: "3.11" + # architecture: "x64" - - name: Install dependencies - run: | - python -m pip install --upgrade pip - pip install nf-core + # - name: Install dependencies + # run: | + # python -m pip install --upgrade pip + # pip install nf-core - - name: Run nf-core lint - env: - GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }} - GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} - GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }} - run: nf-core -l lint_log.txt lint --dir ${GITHUB_WORKSPACE} --markdown lint_results.md + # - name: Run nf-core lint + # env: + # GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }} + # GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} + # GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }} + # run: nf-core -l lint_log.txt lint --dir ${GITHUB_WORKSPACE} --markdown lint_results.md - - name: Save PR number - if: ${{ always() }} - run: echo ${{ github.event.pull_request.number }} > PR_number.txt + # - name: Save PR number + # if: ${{ always() }} + # run: echo ${{ github.event.pull_request.number }} > PR_number.txt - - name: Upload linting log file artifact - if: ${{ always() }} - uses: actions/upload-artifact@v4 - with: - name: linting-logs - path: | - lint_log.txt - lint_results.md - PR_number.txt + # - name: Upload linting log file artifact + # if: ${{ always() }} + # uses: actions/upload-artifact@v4 + # with: + # name: linting-logs + # path: | + # lint_log.txt + # lint_results.md + # PR_number.txt diff --git a/assets/schema_input.json b/assets/schema_input.json index 6aff8b19..d8573b21 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -36,7 +36,7 @@ ] }, "hic_reads": { - "errorMessage": "HiC reads should either be provided as a SRA ID or as a path to paired reads with pattern '*_R{1,2}.(fastq|fq).gz'", + "errorMessage": "HiC reads should either be provided as a SRA ID or as a path to paired reads with pattern '*R{1,2}.(fastq|fq).gz'", "anyOf": [ { "type": "string", @@ -44,7 +44,7 @@ }, { "type": "string", - "pattern": "^\\S+_R\\{1,2\\}\\.f(ast)?q\\.gz$" + "pattern": "^\\S+R\\{1,2\\}\\.f(ast)?q\\.gz$" }, { "type": "string", diff --git a/conf/modules.config b/conf/modules.config index 8202dd27..b1a6da5b 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -47,6 +47,14 @@ process { ] } + withName: NCBI_FCS_GX_KRONA_PLOT { + publishDir = [ + path: { "${params.outdir}/ncbi_fcs_gx" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + withName: RUN_BUSCO { publishDir = [ path: { "${params.outdir}/busco" }, diff --git a/modules/local/assemblathon_stats.nf b/modules/local/assemblathon_stats.nf index 3285e08c..3ef37de5 100644 --- a/modules/local/assemblathon_stats.nf +++ b/modules/local/assemblathon_stats.nf @@ -1,10 +1,11 @@ process ASSEMBLATHON_STATS { tag "${asm_tag}" - label "process_single" + label 'process_single' - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + conda "conda-forge::perl" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ubuntu:20.04': - 'quay.io/nf-core/ubuntu:20.04' }" + 'nf-core/ubuntu:20.04' }" input: tuple val(asm_tag), path(fasta_file) @@ -14,6 +15,9 @@ process ASSEMBLATHON_STATS { path "${asm_tag}_stats.csv" , emit: stats path 'versions.yml' , emit: versions + when: + task.ext.when == null || task.ext.when + script: def VERSION = "github/PlantandFoodResearch/assemblathon2-analysis/a93cba2" """ diff --git a/modules/local/ncbi_fcs_gx_krona_plot.nf b/modules/local/ncbi_fcs_gx_krona_plot.nf new file mode 100644 index 00000000..853689dc --- /dev/null +++ b/modules/local/ncbi_fcs_gx_krona_plot.nf @@ -0,0 +1,42 @@ +process NCBI_FCS_GX_KRONA_PLOT { + tag "${asm_tag}" + label 'process_single' + + container 'docker.io/nanozoo/krona:2.7.1--e7615f7' + + input: + tuple val(asm_tag), path(fcs_gx_taxonomy) + + output: + tuple path("${asm_tag}.inter.tax.rpt.tsv"), path("${asm_tag}.fcs.gx.krona.cut"), path("${asm_tag}.fcs.gx.krona.html") , emit: plot + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "NCBI_FCS_GX_KRONA_PLOT module does not support Conda. Please use Docker / Singularity / Podman instead." + } + """ + cat $fcs_gx_taxonomy \\ + | awk 'NR>1 {print \$1,\$2,\$6,\$7,\$11,\$32}' FS="\\t" OFS="\\t" \\ + > "${asm_tag}.inter.tax.rpt.tsv" + + cat "${asm_tag}.inter.tax.rpt.tsv" \\ + | awk '\$6 !~ /(bogus|repeat|low-coverage|inconclusive)/ {print \$1,\$4,\$5,\$2}' FS="\\t" OFS="\\t" \\ + > "${asm_tag}.fcs.gx.krona.cut" + + cat "${asm_tag}.inter.tax.rpt.tsv" \\ + | awk 'NR>1 && \$6 ~ /(bogus|repeat|low-coverage|inconclusive)/ {print \$1,"0",\$5,\$2}' FS="\\t" OFS="\\t" \\ + >> "${asm_tag}.fcs.gx.krona.cut" + + ktImportTaxonomy -i -o "${asm_tag}.fcs.gx.krona.html" -m "4" "${asm_tag}.fcs.gx.krona.cut" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + KronaTools: \$(ktImportTaxonomy | sed -n '/KronaTools/s/KronaTools//p' | tr -d ' _/[:space:]' | sed 's/-ktImportTaxonomy\\\\//1') + END_VERSIONS + """ +} diff --git a/modules/local/ncbi_fcs_gx_screen_samples.nf b/modules/local/ncbi_fcs_gx_screen_samples.nf index 0ad0d7db..3df915b1 100644 --- a/modules/local/ncbi_fcs_gx_screen_samples.nf +++ b/modules/local/ncbi_fcs_gx_screen_samples.nf @@ -3,9 +3,10 @@ process NCBI_FCS_GX_SCREEN_SAMPLES { label 'process_high' label 'process_long' + conda "bioconda::ncbi-fcs-gx=0.5.0" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://ftp.ncbi.nlm.nih.gov/genomes/TOOLS/FCS/releases/0.4.0/fcs-gx.sif': - 'docker.io/ncbi/fcs-gx:0.4.0' }" + 'https://depot.galaxyproject.org/singularity/ncbi-fcs-gx:0.5.0--h4ac6f70_3': + 'biocontainers/ncbi-fcs-gx:0.5.0--h4ac6f70_3' }" input: path samples @@ -17,17 +18,16 @@ process NCBI_FCS_GX_SCREEN_SAMPLES { path "*.taxonomy.rpt" , emit: fcs_gx_taxonomies path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: - // Exit if running this module with -profile conda / -profile mamba - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - error "NCBI_FCS_GX_SCREEN_SAMPLES module does not support Conda. Please use Docker / Singularity / Podman instead." - } - def VERSION = 0.4 + def VERSION = 0.5 """ for sample_fasta in $samples; do sample_tag=\$(echo "\$sample_fasta" | sed 's/fasta.file.for.//g' | sed 's/.fasta//g') - python3 /app/bin/run_gx --fasta ./\$sample_fasta --out-dir ./ --gx-db $db_path --tax-id "${tax_id}" + run_gx.py --fasta ./\$sample_fasta --out-dir ./ --gx-db $db_path --tax-id "${tax_id}" mv "\${sample_fasta%.fasta}.${tax_id}.fcs_gx_report.txt" "\${sample_tag}.fcs_gx_report.txt" mv "\${sample_fasta%.fasta}.${tax_id}.taxonomy.rpt" "\${sample_tag}.taxonomy.rpt" @@ -40,11 +40,7 @@ process NCBI_FCS_GX_SCREEN_SAMPLES { """ stub: - // Exit if running this module with -profile conda / -profile mamba - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - error "NCBI_FCS_GX_SCREEN_SAMPLES module does not support Conda. Please use Docker / Singularity / Podman instead." - } - def VERSION = 0.4 + def VERSION = 0.5 """ for sample_fasta in $samples; do diff --git a/modules/local/ncbi_fcs_gx_setup_sample.nf b/modules/local/ncbi_fcs_gx_setup_sample.nf new file mode 100644 index 00000000..a5f72385 --- /dev/null +++ b/modules/local/ncbi_fcs_gx_setup_sample.nf @@ -0,0 +1,26 @@ +process NCBI_FCS_GX_SETUP_SAMPLE { + tag "${asm_tag}" + label 'process_single' + + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/ubuntu:20.04': + 'nf-core/ubuntu:20.04' }" + + input: + tuple val(asm_tag), path(fasta_file) + + output: + path 'fasta.file.for.*.fasta', emit: fsata + + when: + task.ext.when == null || task.ext.when + + script: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "NCBI_FCS_GX_SETUP_SAMPLE module does not support Conda. Please use Docker / Singularity / Podman instead." + } + """ + ln -s $fasta_file "fasta.file.for.${asm_tag}.fasta" + """ +} diff --git a/subworkflows/local/ncbi_fcs_gx.nf b/subworkflows/local/ncbi_fcs_gx.nf index 4aa448ed..bcc9350d 100644 --- a/subworkflows/local/ncbi_fcs_gx.nf +++ b/subworkflows/local/ncbi_fcs_gx.nf @@ -1,4 +1,6 @@ -include { NCBI_FCS_GX_SCREEN_SAMPLES } from '../../modules/local/ncbi_fcs_gx_screen_samples' +include { NCBI_FCS_GX_SETUP_SAMPLE } from '../../modules/local/ncbi_fcs_gx_setup_sample' +include { NCBI_FCS_GX_SCREEN_SAMPLES } from '../../modules/local/ncbi_fcs_gx_screen_samples' +include { NCBI_FCS_GX_KRONA_PLOT } from '../../modules/local/ncbi_fcs_gx_krona_plot' workflow NCBI_FCS_GX { take: @@ -51,62 +53,3 @@ workflow NCBI_FCS_GX { gx_taxonomy_plot = ch_gx_taxonomy_plot versions = ch_versions } - -process NCBI_FCS_GX_SETUP_SAMPLE { - tag "${asm_tag}" - label "process_single" - - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/ubuntu:20.04': - 'nf-core/ubuntu:20.04' }" - - input: - tuple val(asm_tag), path(fasta_file) - - output: - path 'fasta.file.for.*.fasta', emit: fsata - - script: - """ - ln -s $fasta_file "fasta.file.for.${asm_tag}.fasta" - """ -} - -process NCBI_FCS_GX_KRONA_PLOT { - tag "${asm_tag}" - label 'process_single' - - container 'docker.io/nanozoo/krona:2.7.1--e7615f7' - publishDir "${params.outdir}/ncbi_fcs_gx", mode: 'copy' - - input: - tuple val(asm_tag), path(fcs_gx_taxonomy) - - output: - tuple path("${asm_tag}.inter.tax.rpt.tsv"), - path("${asm_tag}.fcs.gx.krona.cut"), - path("${asm_tag}.fcs.gx.krona.html") , emit: plot - path "versions.yml" , emit: versions - - script: - """ - cat $fcs_gx_taxonomy \\ - | awk 'NR>1 {print \$1,\$2,\$6,\$7,\$11,\$32}' FS="\\t" OFS="\\t" \\ - > "${asm_tag}.inter.tax.rpt.tsv" - - cat "${asm_tag}.inter.tax.rpt.tsv" \\ - | awk '\$6 !~ /(bogus|repeat|low-coverage|inconclusive)/ {print \$1,\$4,\$5,\$2}' FS="\\t" OFS="\\t" \\ - > "${asm_tag}.fcs.gx.krona.cut" - - cat "${asm_tag}.inter.tax.rpt.tsv" \\ - | awk 'NR>1 && \$6 ~ /(bogus|repeat|low-coverage|inconclusive)/ {print \$1,"0",\$5,\$2}' FS="\\t" OFS="\\t" \\ - >> "${asm_tag}.fcs.gx.krona.cut" - - ktImportTaxonomy -i -o "${asm_tag}.fcs.gx.krona.html" -m "4" "${asm_tag}.fcs.gx.krona.cut" - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - KronaTools: \$(ktImportTaxonomy | sed -n '/KronaTools/s/KronaTools//p' | tr -d ' _/[:space:]' | sed 's/-ktImportTaxonomy\\\\//1') - END_VERSIONS - """ -} diff --git a/tests/stub/FI1.monoploid.seqs.txt b/tests/stub/FI1.monoploid.seqs.txt new file mode 100644 index 00000000..f54b5e6b --- /dev/null +++ b/tests/stub/FI1.monoploid.seqs.txt @@ -0,0 +1,7 @@ +CP031385.1 +CP031386.1 +CP031387.1 +CP031388.1 +CP031389.1 +CP031390.1 +CP031391.1 diff --git a/tests/stub/FI1.seq.labels.tsv b/tests/stub/FI1.seq.labels.tsv new file mode 100644 index 00000000..73636ff6 --- /dev/null +++ b/tests/stub/FI1.seq.labels.tsv @@ -0,0 +1,7 @@ +CP031385.1 FI1_1 +CP031386.1 FI1_2 +CP031387.1 FI1_3 +CP031388.1 FI1_4 +CP031389.1 FI1_5 +CP031390.1 FI1_6 +CP031391.1 FI1_7 diff --git a/tests/stub/TT_2021a.seq.list b/tests/stub/TT_2021a.seq.list new file mode 100644 index 00000000..a51ee157 --- /dev/null +++ b/tests/stub/TT_2021a.seq.list @@ -0,0 +1,7 @@ +CP083245.1 TT1 +CP083246.1 TT2 +CP083247.1 TT3 +CP083248.1 TT4 +CP083249.1 TT5 +CP083250.1 TT6 +CP083251.1 TT7 diff --git a/tests/stub/assemblysheet.csv b/tests/stub/assemblysheet.csv new file mode 100644 index 00000000..86b2de82 --- /dev/null +++ b/tests/stub/assemblysheet.csv @@ -0,0 +1,2 @@ +tag,fasta,gff3,monoploid_ids,hic_reads,synteny_labels +FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz,https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/docs/test_files/FI1.monoploid.seqs.txt,"https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/docs/test_files/hic/stub_hic.R{1,2}.fq.gz",https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/FI1.seq.labels.tsv diff --git a/tests/stub/hic/Dummy_hic.R1.fq.gz b/tests/stub/hic/Dummy_hic.R1.fq.gz new file mode 100644 index 00000000..e69de29b diff --git a/tests/stub/hic/Dummy_hic.R2.fq.gz b/tests/stub/hic/Dummy_hic.R2.fq.gz new file mode 100644 index 00000000..e69de29b From ec4c33dbf313777c32921b982aabc8c7f6fa066e Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 21 Feb 2024 14:51:25 +1300 Subject: [PATCH 20/65] Added stub test to ci --- .github/workflows/ci.yml | 10 +++++----- assets/schema_input.json | 6 +----- lib/WorkflowMain.groovy | 5 +++++ tests/stub/gxdb/test/test-only.gxi | 0 tests/stub/stub.config | 17 +++++++++++++++++ 5 files changed, 28 insertions(+), 10 deletions(-) create mode 100644 tests/stub/gxdb/test/test-only.gxi create mode 100644 tests/stub/stub.config diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 9e9668cc..3527c231 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -26,6 +26,9 @@ jobs: NXF_VER: - "23.04.0" - "latest-everything" + RUN_CONFIG: + - "-profile test,docker" + - "-profile docker -c ./tests/stub/stub.config -stub" steps: - name: Check out pipeline code uses: actions/checkout@v4 @@ -33,11 +36,8 @@ jobs: - name: Install Nextflow uses: nf-core/setup-nextflow@v1 with: - version: "${{ matrix.NXF_VER }}" + version: ${{ matrix.NXF_VER }} - name: Run pipeline with test data - # TODO nf-core: You can customise CI pipeline run tests as required - # For example: adding multiple test runs with different parameters - # Remember that you can parallelise this by using strategy.matrix run: | - nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results + nextflow run ${GITHUB_WORKSPACE} --outdir ./results ${{ matrix.RUN_CONFIG }} diff --git a/assets/schema_input.json b/assets/schema_input.json index d8573b21..88f9a89b 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -57,11 +57,7 @@ "anyOf": [ { "type": "string", - "pattern": "^SR\\w+$" - }, - { - "type": "string", - "pattern": "^\\S+_R\\{1,2\\}\\.f(ast)?q\\.gz$" + "pattern": "^\\S+\\.tsv$" }, { "type": "string", diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy index 2a637074..3a403aac 100755 --- a/lib/WorkflowMain.groovy +++ b/lib/WorkflowMain.groovy @@ -47,6 +47,11 @@ class WorkflowMain { if (!params.ncbi_fcs_gx_skip && !params.ncbi_fcs_gx_tax_id) { Nextflow.error('ncbi_fcs_gx_tax_id must be provided when executing NCBI FCS GX') } + + // Check for ncbi_fcs_gx_db_path + if (!params.ncbi_fcs_gx_skip && !params.ncbi_fcs_gx_db_path) { + Nextflow.error('ncbi_fcs_gx_db_path must be provided when executing NCBI FCS GX') + } } // // Get attribute from genome config file e.g. fasta diff --git a/tests/stub/gxdb/test/test-only.gxi b/tests/stub/gxdb/test/test-only.gxi new file mode 100644 index 00000000..e69de29b diff --git a/tests/stub/stub.config b/tests/stub/stub.config new file mode 100644 index 00000000..004f8bfb --- /dev/null +++ b/tests/stub/stub.config @@ -0,0 +1,17 @@ +params { + config_profile_name = 'Full stub test' + config_profile_description = 'Full test of the pipeline in stub mode' + + input = 'https://raw.githubusercontent.com/Plant-Food-Research-Open/assemblyqc/dev/tests/stub/assemblysheet.csv' + + ncbi_fcs_adaptor_skip = false + + ncbi_fcs_gx_skip = false + ncbi_fcs_gx_tax_id = 12 + ncbi_fcs_gx_db_path = 'https://raw.githubusercontent.com/Plant-Food-Research-Open/assemblyqc/dev/tests/stub/gxdb/test' + + // Limit resources so that this can run on GitHub Actions + max_cpus = 2 + max_memory = '6.GB' + max_time = '6.h' +} From 75faf2ee84368a260c73f70626fec6f40d791127 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 21 Feb 2024 14:59:27 +1300 Subject: [PATCH 21/65] Updated stub test config --- tests/stub/stub.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/tests/stub/stub.config b/tests/stub/stub.config index 004f8bfb..3b302579 100644 --- a/tests/stub/stub.config +++ b/tests/stub/stub.config @@ -8,7 +8,7 @@ params { ncbi_fcs_gx_skip = false ncbi_fcs_gx_tax_id = 12 - ncbi_fcs_gx_db_path = 'https://raw.githubusercontent.com/Plant-Food-Research-Open/assemblyqc/dev/tests/stub/gxdb/test' + ncbi_fcs_gx_db_path = 'tests/stub/gxdb/test' // Limit resources so that this can run on GitHub Actions max_cpus = 2 From 64eebf32ab4ff81a1b7d625d6532b03f2ea7160b Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 22 Feb 2024 09:23:47 +1300 Subject: [PATCH 22/65] Added fasta_busco_plot workflow --- conf/modules.config | 10 ++++- lib/WorkflowMain.groovy | 5 +++ modules/local/busco.nf | 54 ++++++++++++++++++++++++++ modules/local/busco_plot.nf | 28 +++++++++++++ nextflow_schema.json | 2 +- subworkflows/local/fasta_busco_plot.nf | 32 +++++++++++++++ tests/stub/stub.config | 3 ++ workflows/assemblyqc.nf | 24 ++++++++++++ 8 files changed, 156 insertions(+), 2 deletions(-) create mode 100644 modules/local/busco.nf create mode 100644 modules/local/busco_plot.nf create mode 100644 subworkflows/local/fasta_busco_plot.nf diff --git a/conf/modules.config b/conf/modules.config index b1a6da5b..d4d26b4e 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -55,7 +55,7 @@ process { ] } - withName: RUN_BUSCO { + withName: BUSCO { publishDir = [ path: { "${params.outdir}/busco" }, mode: params.publish_dir_mode, @@ -63,6 +63,14 @@ process { ] } + withName: BUSCO_PLOT { + publishDir = [ + path: { "${params.outdir}" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + withName: RUN_KRAKEN2 { publishDir = [ path: { "${params.outdir}/kraken2" }, diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy index 3a403aac..c6c8b504 100755 --- a/lib/WorkflowMain.groovy +++ b/lib/WorkflowMain.groovy @@ -52,6 +52,11 @@ class WorkflowMain { if (!params.ncbi_fcs_gx_skip && !params.ncbi_fcs_gx_db_path) { Nextflow.error('ncbi_fcs_gx_db_path must be provided when executing NCBI FCS GX') } + + // Check for busco_lineage_datasets + if (!params.busco_skip && !params.busco_lineage_datasets) { + Nextflow.error('busco_lineage_datasets must be provided when executing BUSCO') + } } // // Get attribute from genome config file e.g. fasta diff --git a/modules/local/busco.nf b/modules/local/busco.nf new file mode 100644 index 00000000..adcaf678 --- /dev/null +++ b/modules/local/busco.nf @@ -0,0 +1,54 @@ +process BUSCO { + tag "${asm_tag}:${lineage_dataset}" + label 'process_high' + + conda "bioconda::busco=5.2.2" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/busco:5.2.2--pyhdfd78af_0': + 'quay.io/biocontainers/busco:5.2.2--pyhdfd78af_0' }" + + input: + tuple val(asm_tag), path(fasta_file) + val lineage_dataset + val mode + val download_path + + output: + path "${asm_tag}/short_summary.specific.${lineage_dataset}.${asm_tag}_${lineage_initials}.txt" , emit: summary + path "versions.yml" , emit: versions + + script: + def lineages_path = download_path ? "--download_path ${download_path}" : '' + lineage_initials = "${lineage_dataset}".split("_")[0] + + """ + busco \\ + -m ${mode} \\ + -o ${asm_tag} \\ + -i $fasta_file \\ + -l ${lineage_dataset} \\ + --update-data \\ + $lineages_path \\ + -c ${task.cpus} + + mv $asm_tag/short_summary.specific.${lineage_dataset}.${asm_tag}.txt \\ + $asm_tag/short_summary.specific.${lineage_dataset}.${asm_tag}_${lineage_initials}.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + busco: \$( busco --version 2>&1 | sed 's/^BUSCO //' ) + END_VERSIONS + """ + + stub: + lineage_initials = "${lineage_dataset}".split("_")[0] + """ + mkdir -p $asm_tag + touch $asm_tag/short_summary.specific.${lineage_dataset}.${asm_tag}_${lineage_initials}.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + busco: \$( busco --version 2>&1 | sed 's/^BUSCO //' ) + END_VERSIONS + """ +} diff --git a/modules/local/busco_plot.nf b/modules/local/busco_plot.nf new file mode 100644 index 00000000..24bfd386 --- /dev/null +++ b/modules/local/busco_plot.nf @@ -0,0 +1,28 @@ +process BUSCO_PLOT { + tag 'all summaries' + label 'process_single' + + conda "bioconda::busco=5.2.2" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/busco:5.2.2--pyhdfd78af_0': + 'quay.io/biocontainers/busco:5.2.2--pyhdfd78af_0' }" + + input: + path "short_summary.*", stageAs: 'busco/*' + + output: + path 'busco/*.png', emit: png + + script: + """ + generate_plot.py \\ + -wd ./busco + """ + + stub: + """ + mkdir -p busco + + touch busco/summary_plot.png + """ +} diff --git a/nextflow_schema.json b/nextflow_schema.json index 94599989..add593cc 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -107,7 +107,7 @@ "busco_lineage_datasets": { "type": "string", "help_text": "Each input assembly is assessed against each lineage. It should be provided as a space-separated list of lineages: 'fungi_odb10 microsporidia_odb10' ", - "pattern": "^(\\w+_odb10\\s)+\\w+_odb10$", + "pattern": "^(\\w+_odb10\\s)*\\w+_odb10$", "description": "BUSCO lineages" }, "busco_download_path": { diff --git a/subworkflows/local/fasta_busco_plot.nf b/subworkflows/local/fasta_busco_plot.nf new file mode 100644 index 00000000..7a8bd8fd --- /dev/null +++ b/subworkflows/local/fasta_busco_plot.nf @@ -0,0 +1,32 @@ +include { BUSCO } from '../../modules/local/busco' +include { BUSCO_PLOT } from '../../modules/local/busco_plot' + +workflow FASTA_BUSCO_PLOT { + take: + tuple_of_hap_file // Channel + lineage // val + mode // val + download_path // val; Use [] to use work directory. Useful on AWS + + main: + // MODULE: BUSCO + BUSCO( + tuple_of_hap_file, + lineage, + mode, + download_path + ) + + ch_busco_summaries = BUSCO.out.summary + | collect + + // MODULE: BUSCO_PLOT + BUSCO_PLOT ( ch_busco_summaries ) + + ch_busco_plot = BUSCO_PLOT.out.png + + emit: + summary = BUSCO.out.summary + plot = ch_busco_plot + versions = Channel.empty().mix(BUSCO.out.versions.first()) +} diff --git a/tests/stub/stub.config b/tests/stub/stub.config index 3b302579..371b42ac 100644 --- a/tests/stub/stub.config +++ b/tests/stub/stub.config @@ -10,6 +10,9 @@ params { ncbi_fcs_gx_tax_id = 12 ncbi_fcs_gx_db_path = 'tests/stub/gxdb/test' + busco_skip = false + busco_lineage_datasets = 'fungi_odb10 hypocreales_odb10' + // Limit resources so that this can run on GitHub Actions max_cpus = 2 max_memory = '6.GB' diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index 34f71156..4758b636 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -32,6 +32,7 @@ include { GFF3_VALIDATE } from '../subworkflows/pfr/gff3_val include { NCBI_FCS_ADAPTOR } from '../modules/local/ncbi_fcs_adaptor' include { NCBI_FCS_GX } from '../subworkflows/local/ncbi_fcs_gx' include { ASSEMBLATHON_STATS } from '../modules/local/assemblathon_stats' +include { FASTA_BUSCO_PLOT } from '../subworkflows/local/fasta_busco_plot' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -253,6 +254,29 @@ workflow ASSEMBLYQC { ch_assemblathon_stats = ASSEMBLATHON_STATS.out.stats ch_versions = ch_versions.mix(ASSEMBLATHON_STATS.out.versions.first()) + // SUBWORKFLOW: FASTA_BUSCO_PLOT + ch_busco_inputs = params.busco_skip + ? Channel.empty() + : ch_clean_assembly + | combine( + Channel.of(params.busco_lineage_datasets) + | map { it.split(' ') } + | flatten + ) + | map { tag, fa, lineage -> + [ tag, file(fa, checkIfExists: true), lineage ] + } + FASTA_BUSCO_PLOT( + ch_busco_inputs.map { tag, fa, lineage -> [ tag, fa ] }, + ch_busco_inputs.map { tag, fa, lineage -> lineage }, + params.busco_mode, + params.busco_download_path ?: [] + ) + + ch_busco_summary = FASTA_BUSCO_PLOT.out.summary + ch_busco_plot = FASTA_BUSCO_PLOT.out.plot + ch_versions = ch_versions.mix(FASTA_BUSCO_PLOT.out.versions) + // MODULE: CUSTOM_DUMPSOFTWAREVERSIONS CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') From 92c5da164bab0687796da0c941b2d5b93e2b497c Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 22 Feb 2024 09:44:08 +1300 Subject: [PATCH 23/65] Added fasta_explore_search_plot_tidk subworkflow --- modules.json | 34 ++ modules/local/busco.nf | 9 +- modules/local/busco_plot.nf | 22 +- modules/nf-core/seqkit/seq/environment.yml | 9 + modules/nf-core/seqkit/seq/main.nf | 63 +++ modules/nf-core/seqkit/seq/meta.yml | 48 +++ modules/nf-core/seqkit/seq/tests/main.nf.test | 149 +++++++ .../seqkit/seq/tests/main.nf.test.snap | 126 ++++++ .../nf-core/seqkit/seq/tests/nextflow.config | 3 + modules/nf-core/seqkit/seq/tests/tags.yml | 2 + modules/nf-core/seqkit/sort/environment.yml | 9 + modules/nf-core/seqkit/sort/main.nf | 63 +++ modules/nf-core/seqkit/sort/meta.yml | 45 ++ .../nf-core/seqkit/sort/tests/main.nf.test | 149 +++++++ .../seqkit/sort/tests/main.nf.test.snap | 126 ++++++ .../nf-core/seqkit/sort/tests/nextflow.config | 3 + modules/nf-core/seqkit/sort/tests/tags.yml | 2 + modules/nf-core/tidk/explore/environment.yml | 9 + modules/nf-core/tidk/explore/main.nf | 57 +++ modules/nf-core/tidk/explore/meta.yml | 52 +++ .../nf-core/tidk/explore/tests/main.nf.test | 86 ++++ .../tidk/explore/tests/main.nf.test.snap | 68 +++ .../tidk/explore/tests/nextflow.config | 3 + modules/nf-core/tidk/explore/tests/tags.yml | 2 + modules/nf-core/tidk/plot/environment.yml | 9 + modules/nf-core/tidk/plot/main.nf | 47 +++ modules/nf-core/tidk/plot/meta.yml | 47 +++ modules/nf-core/tidk/plot/tests/main.nf.test | 74 ++++ .../nf-core/tidk/plot/tests/main.nf.test.snap | 68 +++ modules/nf-core/tidk/plot/tests/tags.yml | 2 + modules/nf-core/tidk/search/environment.yml | 9 + modules/nf-core/tidk/search/main.nf | 62 +++ modules/nf-core/tidk/search/meta.yml | 51 +++ .../nf-core/tidk/search/tests/main.nf.test | 119 ++++++ .../tidk/search/tests/main.nf.test.snap | 36 ++ .../nf-core/tidk/search/tests/nextflow.config | 3 + modules/nf-core/tidk/search/tests/tags.yml | 2 + subworkflows/local/fasta_busco_plot.nf | 7 +- .../fasta_explore_search_plot_tidk/main.nf | 90 ++++ .../fasta_explore_search_plot_tidk/meta.yml | 74 ++++ .../tests/main.nf.test | 119 ++++++ .../tests/main.nf.test.snap | 389 ++++++++++++++++++ .../tests/nextflow.config | 19 + .../tests/tags.yml | 2 + tests/stub/stub.config | 2 + workflows/assemblyqc.nf | 26 ++ 46 files changed, 2388 insertions(+), 8 deletions(-) create mode 100644 modules/nf-core/seqkit/seq/environment.yml create mode 100644 modules/nf-core/seqkit/seq/main.nf create mode 100644 modules/nf-core/seqkit/seq/meta.yml create mode 100644 modules/nf-core/seqkit/seq/tests/main.nf.test create mode 100644 modules/nf-core/seqkit/seq/tests/main.nf.test.snap create mode 100644 modules/nf-core/seqkit/seq/tests/nextflow.config create mode 100644 modules/nf-core/seqkit/seq/tests/tags.yml create mode 100644 modules/nf-core/seqkit/sort/environment.yml create mode 100644 modules/nf-core/seqkit/sort/main.nf create mode 100644 modules/nf-core/seqkit/sort/meta.yml create mode 100644 modules/nf-core/seqkit/sort/tests/main.nf.test create mode 100644 modules/nf-core/seqkit/sort/tests/main.nf.test.snap create mode 100644 modules/nf-core/seqkit/sort/tests/nextflow.config create mode 100644 modules/nf-core/seqkit/sort/tests/tags.yml create mode 100644 modules/nf-core/tidk/explore/environment.yml create mode 100644 modules/nf-core/tidk/explore/main.nf create mode 100644 modules/nf-core/tidk/explore/meta.yml create mode 100644 modules/nf-core/tidk/explore/tests/main.nf.test create mode 100644 modules/nf-core/tidk/explore/tests/main.nf.test.snap create mode 100644 modules/nf-core/tidk/explore/tests/nextflow.config create mode 100644 modules/nf-core/tidk/explore/tests/tags.yml create mode 100644 modules/nf-core/tidk/plot/environment.yml create mode 100644 modules/nf-core/tidk/plot/main.nf create mode 100644 modules/nf-core/tidk/plot/meta.yml create mode 100644 modules/nf-core/tidk/plot/tests/main.nf.test create mode 100644 modules/nf-core/tidk/plot/tests/main.nf.test.snap create mode 100644 modules/nf-core/tidk/plot/tests/tags.yml create mode 100644 modules/nf-core/tidk/search/environment.yml create mode 100644 modules/nf-core/tidk/search/main.nf create mode 100644 modules/nf-core/tidk/search/meta.yml create mode 100644 modules/nf-core/tidk/search/tests/main.nf.test create mode 100644 modules/nf-core/tidk/search/tests/main.nf.test.snap create mode 100644 modules/nf-core/tidk/search/tests/nextflow.config create mode 100644 modules/nf-core/tidk/search/tests/tags.yml create mode 100644 subworkflows/nf-core/fasta_explore_search_plot_tidk/main.nf create mode 100644 subworkflows/nf-core/fasta_explore_search_plot_tidk/meta.yml create mode 100644 subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test create mode 100644 subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test.snap create mode 100644 subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/nextflow.config create mode 100644 subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/tags.yml diff --git a/modules.json b/modules.json index ef57949b..c76c0439 100644 --- a/modules.json +++ b/modules.json @@ -54,6 +54,40 @@ "branch": "master", "git_sha": "3a5fef109d113b4997c9822198664ca5f2716208", "installed_by": ["modules"] + }, + "seqkit/seq": { + "branch": "master", + "git_sha": "687ad41c14008d3d55cf7c2ffacebe6a057211a4", + "installed_by": ["fasta_explore_search_plot_tidk"] + }, + "seqkit/sort": { + "branch": "master", + "git_sha": "ffcdfb354f8c8e59bdccc1a4249f0f3b232c2a3d", + "installed_by": ["fasta_explore_search_plot_tidk"] + }, + "tidk/explore": { + "branch": "master", + "git_sha": "fad335b31b32d78f89a8340373d3ccfc0f2b18b5", + "installed_by": ["fasta_explore_search_plot_tidk"] + }, + "tidk/plot": { + "branch": "master", + "git_sha": "d54cd1d823f151854825da8ffe7289bcbf77206b", + "installed_by": ["fasta_explore_search_plot_tidk"] + }, + "tidk/search": { + "branch": "master", + "git_sha": "669a329f4aa37f5b7f03776c2ed1cd0ef122c626", + "installed_by": ["fasta_explore_search_plot_tidk"] + } + } + }, + "subworkflows": { + "nf-core": { + "fasta_explore_search_plot_tidk": { + "branch": "master", + "git_sha": "2b21fbeb20ad9f17612f4a3dd7b12971513f08d5", + "installed_by": ["subworkflows"] } } } diff --git a/modules/local/busco.nf b/modules/local/busco.nf index adcaf678..c7538c8e 100644 --- a/modules/local/busco.nf +++ b/modules/local/busco.nf @@ -2,10 +2,10 @@ process BUSCO { tag "${asm_tag}:${lineage_dataset}" label 'process_high' - conda "bioconda::busco=5.2.2" + conda "bioconda::busco=5.6.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/busco:5.2.2--pyhdfd78af_0': - 'quay.io/biocontainers/busco:5.2.2--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/busco:5.6.1--pyhdfd78af_0': + 'biocontainers/busco:5.6.1--pyhdfd78af_0' }" input: tuple val(asm_tag), path(fasta_file) @@ -17,6 +17,9 @@ process BUSCO { path "${asm_tag}/short_summary.specific.${lineage_dataset}.${asm_tag}_${lineage_initials}.txt" , emit: summary path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def lineages_path = download_path ? "--download_path ${download_path}" : '' lineage_initials = "${lineage_dataset}".split("_")[0] diff --git a/modules/local/busco_plot.nf b/modules/local/busco_plot.nf index 24bfd386..e8f70189 100644 --- a/modules/local/busco_plot.nf +++ b/modules/local/busco_plot.nf @@ -2,21 +2,30 @@ process BUSCO_PLOT { tag 'all summaries' label 'process_single' - conda "bioconda::busco=5.2.2" + conda "bioconda::busco=5.6.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/busco:5.2.2--pyhdfd78af_0': - 'quay.io/biocontainers/busco:5.2.2--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/busco:5.6.1--pyhdfd78af_0': + 'biocontainers/busco:5.6.1--pyhdfd78af_0' }" input: path "short_summary.*", stageAs: 'busco/*' output: - path 'busco/*.png', emit: png + path 'busco/*.png' , emit: png + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when script: """ generate_plot.py \\ -wd ./busco + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + busco: \$( busco --version 2>&1 | sed 's/^BUSCO //' ) + END_VERSIONS """ stub: @@ -24,5 +33,10 @@ process BUSCO_PLOT { mkdir -p busco touch busco/summary_plot.png + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + busco: \$( busco --version 2>&1 | sed 's/^BUSCO //' ) + END_VERSIONS """ } diff --git a/modules/nf-core/seqkit/seq/environment.yml b/modules/nf-core/seqkit/seq/environment.yml new file mode 100644 index 00000000..9019d269 --- /dev/null +++ b/modules/nf-core/seqkit/seq/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "seqkit_seq" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::seqkit=2.6.1" diff --git a/modules/nf-core/seqkit/seq/main.nf b/modules/nf-core/seqkit/seq/main.nf new file mode 100644 index 00000000..7fc742b3 --- /dev/null +++ b/modules/nf-core/seqkit/seq/main.nf @@ -0,0 +1,63 @@ +process SEQKIT_SEQ { + tag "$meta.id" + label 'process_low' + // File IO can be a bottleneck. See: https://bioinf.shenwei.me/seqkit/usage/#parallelization-of-cpu-intensive-jobs + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/seqkit:2.6.1--h9ee0642_0': + 'biocontainers/seqkit:2.6.1--h9ee0642_0' }" + + input: + tuple val(meta), path(fastx) + + output: + tuple val(meta), path("${prefix}.*") , emit: fastx + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + prefix = task.ext.prefix ?: "${meta.id}" + def extension = "fastq" + if ("$fastx" ==~ /.+\.fasta|.+\.fasta.gz|.+\.fa|.+\.fa.gz|.+\.fas|.+\.fas.gz|.+\.fna|.+\.fna.gz|.+\.fsa|.+\.fsa.gz/ ) { + extension = "fasta" + } + extension = fastx.toString().endsWith('.gz') ? "${extension}.gz" : extension + def call_gzip = extension.endsWith('.gz') ? "| gzip -c $args2" : '' + if("${prefix}.${extension}" == "$fastx") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ + seqkit \\ + seq \\ + --threads $task.cpus \\ + $args \\ + $fastx \\ + $call_gzip \\ + > ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + seqkit: \$(seqkit version | cut -d' ' -f2) + END_VERSIONS + """ + + stub: + prefix = task.ext.prefix ?: "${meta.id}" + def extension = "fastq" + if ("$fastx" ==~ /.+\.fasta|.+\.fasta.gz|.+\.fa|.+\.fa.gz|.+\.fas|.+\.fas.gz|.+\.fna|.+\.fna.gz|.+\.fsa|.+\.fsa.gz/ ) { + extension = "fasta" + } + extension = fastx.toString().endsWith('.gz') ? "${extension}.gz" : extension + if("${prefix}.${extension}" == "$fastx") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ + touch ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + seqkit: \$(seqkit version | cut -d' ' -f2) + END_VERSIONS + """ +} diff --git a/modules/nf-core/seqkit/seq/meta.yml b/modules/nf-core/seqkit/seq/meta.yml new file mode 100644 index 00000000..8d4e2b16 --- /dev/null +++ b/modules/nf-core/seqkit/seq/meta.yml @@ -0,0 +1,48 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "seqkit_seq" +description: Transforms sequences (extract ID, filter by length, remove gaps, reverse complement...) +keywords: + - genomics + - fasta + - fastq + - transform + - filter + - gaps + - complement +tools: + - "seqkit": + description: "A cross-platform and ultrafast toolkit for FASTA/Q file manipulation" + homepage: "https://bioinf.shenwei.me/seqkit/" + documentation: "https://bioinf.shenwei.me/seqkit/usage/" + tool_dev_url: "https://github.com/shenwei356/seqkit" + doi: "10.1371/journal.pone.0163962" + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fastx: + type: file + description: Input fasta/fastq file + pattern: "*.{fsa,fas,fa,fasta,fastq,fq,fsa.gz,fas.gz,fa.gz,fasta.gz,fastq.gz,fq.gz}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fastx: + type: file + description: Output fasta/fastq file + pattern: "*.{fasta,fasta.gz,fastq,fastq.gz}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/nf-core/seqkit/seq/tests/main.nf.test b/modules/nf-core/seqkit/seq/tests/main.nf.test new file mode 100644 index 00000000..aa9c283e --- /dev/null +++ b/modules/nf-core/seqkit/seq/tests/main.nf.test @@ -0,0 +1,149 @@ +nextflow_process { + + name "Test Process SEQKIT_SEQ" + script "../main.nf" + process "SEQKIT_SEQ" + config './nextflow.config' + + tag "modules" + tag "modules_nfcore" + tag "seqkit" + tag "seqkit/seq" + + test("sarscov2-genome_fasta") { + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("sarscov2-genome_fasta_gz") { + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta_gz'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("sarscov2-test_1_fastq_gz") { + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("file_name_conflict-fail_with_error") { + when { + process { + """ + input[0] = [ + [ id:'test_1' ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert !process.success }, + { assert process.stdout.toString().contains("Input and output names are the same") } + ) + } + + } + + test("sarscov2-genome_fasta-stub") { + + options '-stub' + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("file_name_conflict-fail_with_error-stub") { + + options '-stub' + + when { + process { + """ + input[0] = [ + [ id:'genome' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert !process.success }, + { assert process.stdout.toString().contains("Input and output names are the same") } + ) + } + + } + +} diff --git a/modules/nf-core/seqkit/seq/tests/main.nf.test.snap b/modules/nf-core/seqkit/seq/tests/main.nf.test.snap new file mode 100644 index 00000000..91b4d17a --- /dev/null +++ b/modules/nf-core/seqkit/seq/tests/main.nf.test.snap @@ -0,0 +1,126 @@ +{ + "sarscov2-genome_fasta-stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.fasta:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" + ], + "fastx": [ + [ + { + "id": "test" + }, + "test.fasta:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" + ] + } + ], + "timestamp": "2023-12-18T10:34:00.37449" + }, + "versions": { + "content": [ + [ + "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" + ] + ], + "timestamp": "2023-12-17T13:56:53.318962" + }, + "sarscov2-test_1_fastq_gz": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.fastq.gz:md5,4161df271f9bfcd25d5845a1e220dbec" + ] + ], + "1": [ + "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" + ], + "fastx": [ + [ + { + "id": "test" + }, + "test.fastq.gz:md5,4161df271f9bfcd25d5845a1e220dbec" + ] + ], + "versions": [ + "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" + ] + } + ], + "timestamp": "2023-12-18T10:33:53.528342" + }, + "sarscov2-genome_fasta": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.fasta:md5,483f4a5dfe60171c86ee9b7e6dff908b" + ] + ], + "1": [ + "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" + ], + "fastx": [ + [ + { + "id": "test" + }, + "test.fasta:md5,483f4a5dfe60171c86ee9b7e6dff908b" + ] + ], + "versions": [ + "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" + ] + } + ], + "timestamp": "2023-12-18T10:33:44.757686" + }, + "sarscov2-genome_fasta_gz": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.fasta.gz:md5,483f4a5dfe60171c86ee9b7e6dff908b" + ] + ], + "1": [ + "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" + ], + "fastx": [ + [ + { + "id": "test" + }, + "test.fasta.gz:md5,483f4a5dfe60171c86ee9b7e6dff908b" + ] + ], + "versions": [ + "versions.yml:md5,03e95c09e4faae889c3d516b2d4b9d7f" + ] + } + ], + "timestamp": "2023-12-18T10:33:49.115171" + } +} \ No newline at end of file diff --git a/modules/nf-core/seqkit/seq/tests/nextflow.config b/modules/nf-core/seqkit/seq/tests/nextflow.config new file mode 100644 index 00000000..d8e3c66a --- /dev/null +++ b/modules/nf-core/seqkit/seq/tests/nextflow.config @@ -0,0 +1,3 @@ +process { + ext.args2 = '-n' +} diff --git a/modules/nf-core/seqkit/seq/tests/tags.yml b/modules/nf-core/seqkit/seq/tests/tags.yml new file mode 100644 index 00000000..5eeca7e3 --- /dev/null +++ b/modules/nf-core/seqkit/seq/tests/tags.yml @@ -0,0 +1,2 @@ +seqkit/seq: + - "modules/nf-core/seqkit/seq/**" diff --git a/modules/nf-core/seqkit/sort/environment.yml b/modules/nf-core/seqkit/sort/environment.yml new file mode 100644 index 00000000..820c5707 --- /dev/null +++ b/modules/nf-core/seqkit/sort/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "seqkit_sort" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::seqkit=2.6.1" diff --git a/modules/nf-core/seqkit/sort/main.nf b/modules/nf-core/seqkit/sort/main.nf new file mode 100644 index 00000000..08f86eb1 --- /dev/null +++ b/modules/nf-core/seqkit/sort/main.nf @@ -0,0 +1,63 @@ +process SEQKIT_SORT { + tag "$meta.id" + label 'process_low' + // File IO can be a bottleneck. See: https://bioinf.shenwei.me/seqkit/usage/#parallelization-of-cpu-intensive-jobs + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/seqkit:2.6.1--h9ee0642_0': + 'biocontainers/seqkit:2.6.1--h9ee0642_0' }" + + input: + tuple val(meta), path(fastx) + + output: + tuple val(meta), path("${prefix}.*") , emit: fastx + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + prefix = task.ext.prefix ?: "${meta.id}" + def extension = "fastq" + if ("$fastx" ==~ /.+\.fasta|.+\.fasta.gz|.+\.fa|.+\.fa.gz|.+\.fas|.+\.fas.gz|.+\.fna|.+\.fna.gz|.+\.fsa|.+\.fsa.gz/ ) { + extension = "fasta" + } + extension = fastx.toString().endsWith('.gz') ? "${extension}.gz" : extension + def call_gzip = extension.endsWith('.gz') ? "| gzip -c $args2 " : '' + if("${prefix}.${extension}" == "$fastx") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ + seqkit \\ + sort \\ + --threads $task.cpus \\ + $args \\ + $fastx \\ + $call_gzip \\ + > ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + seqkit: \$(seqkit version | cut -d' ' -f2) + END_VERSIONS + """ + + stub: + prefix = task.ext.prefix ?: "${meta.id}" + def extension = "fastq" + if ("$fastx" ==~ /.+\.fasta|.+\.fasta.gz|.+\.fa|.+\.fa.gz|.+\.fas|.+\.fas.gz|.+\.fna|.+\.fna.gz|.+\.fsa|.+\.fsa.gz/ ) { + extension = "fasta" + } + extension = fastx.toString().endsWith('.gz') ? "${extension}.gz" : extension + if("${prefix}.${extension}" == "$fastx") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ + touch ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + seqkit: \$(seqkit version | cut -d' ' -f2) + END_VERSIONS + """ +} diff --git a/modules/nf-core/seqkit/sort/meta.yml b/modules/nf-core/seqkit/sort/meta.yml new file mode 100644 index 00000000..2e61ce15 --- /dev/null +++ b/modules/nf-core/seqkit/sort/meta.yml @@ -0,0 +1,45 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "seqkit_sort" +description: Sorts sequences by id/name/sequence/length +keywords: + - genomics + - fasta + - fastq + - sort +tools: + - "seqkit": + description: "A cross-platform and ultrafast toolkit for FASTA/Q file manipulation" + homepage: "https://bioinf.shenwei.me/seqkit/" + documentation: "https://bioinf.shenwei.me/seqkit/usage/" + tool_dev_url: "https://github.com/shenwei356/seqkit" + doi: "10.1371/journal.pone.0163962" + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fastx: + type: file + description: Input fasta/fastq file + pattern: "*.{fsa,fas,fa,fasta,fastq,fq,fsa.gz,fas.gz,fa.gz,fasta.gz,fastq.gz,fq.gz}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fastx: + type: file + description: Output fasta/fastq file + pattern: "*.{fasta.gz,fastq.gz}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/nf-core/seqkit/sort/tests/main.nf.test b/modules/nf-core/seqkit/sort/tests/main.nf.test new file mode 100644 index 00000000..0c2f4e2d --- /dev/null +++ b/modules/nf-core/seqkit/sort/tests/main.nf.test @@ -0,0 +1,149 @@ +nextflow_process { + + name "Test Process SEQKIT_SORT" + script "../main.nf" + process "SEQKIT_SORT" + config './nextflow.config' + + tag "modules" + tag "modules_nfcore" + tag "seqkit" + tag "seqkit/sort" + + test("sarscov2-genome_fasta") { + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("sarscov2-genome_fasta_gz") { + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta_gz'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("sarscov2-test_1_fastq_gz") { + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("file_name_conflict-fail_with_error") { + when { + process { + """ + input[0] = [ + [ id:'test_1' ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert !process.success }, + { assert process.stdout.toString().contains("Input and output names are the same,") } + ) + } + + } + + test("sarscov2-genome_fasta-stub") { + + options '-stub' + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("file_name_conflict-fail_with_error-stub") { + + options '-stub' + + when { + process { + """ + input[0] = [ + [ id:'genome' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert !process.success }, + { assert process.stdout.toString().contains("Input and output names are the same") } + ) + } + + } + +} diff --git a/modules/nf-core/seqkit/sort/tests/main.nf.test.snap b/modules/nf-core/seqkit/sort/tests/main.nf.test.snap new file mode 100644 index 00000000..94e2cb35 --- /dev/null +++ b/modules/nf-core/seqkit/sort/tests/main.nf.test.snap @@ -0,0 +1,126 @@ +{ + "sarscov2-genome_fasta-stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.fasta:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" + ], + "fastx": [ + [ + { + "id": "test" + }, + "test.fasta:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" + ] + } + ], + "timestamp": "2023-12-18T10:07:19.28815" + }, + "versions": { + "content": [ + [ + "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" + ] + ], + "timestamp": "2023-12-18T10:07:15.341516" + }, + "sarscov2-test_1_fastq_gz": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.fastq.gz:md5,80d9fd1976648214f44e42bc3d705972" + ] + ], + "1": [ + "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" + ], + "fastx": [ + [ + { + "id": "test" + }, + "test.fastq.gz:md5,80d9fd1976648214f44e42bc3d705972" + ] + ], + "versions": [ + "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" + ] + } + ], + "timestamp": "2023-12-18T10:12:25.704893" + }, + "sarscov2-genome_fasta": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.fasta:md5,483f4a5dfe60171c86ee9b7e6dff908b" + ] + ], + "1": [ + "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" + ], + "fastx": [ + [ + { + "id": "test" + }, + "test.fasta:md5,483f4a5dfe60171c86ee9b7e6dff908b" + ] + ], + "versions": [ + "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" + ] + } + ], + "timestamp": "2023-12-18T10:07:15.293713" + }, + "sarscov2-genome_fasta_gz": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.fasta.gz:md5,483f4a5dfe60171c86ee9b7e6dff908b" + ] + ], + "1": [ + "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" + ], + "fastx": [ + [ + { + "id": "test" + }, + "test.fasta.gz:md5,483f4a5dfe60171c86ee9b7e6dff908b" + ] + ], + "versions": [ + "versions.yml:md5,ffb1083944323f1b40bd5699cfa8b998" + ] + } + ], + "timestamp": "2023-12-18T10:09:20.306713" + } +} \ No newline at end of file diff --git a/modules/nf-core/seqkit/sort/tests/nextflow.config b/modules/nf-core/seqkit/sort/tests/nextflow.config new file mode 100644 index 00000000..d8e3c66a --- /dev/null +++ b/modules/nf-core/seqkit/sort/tests/nextflow.config @@ -0,0 +1,3 @@ +process { + ext.args2 = '-n' +} diff --git a/modules/nf-core/seqkit/sort/tests/tags.yml b/modules/nf-core/seqkit/sort/tests/tags.yml new file mode 100644 index 00000000..c839b58c --- /dev/null +++ b/modules/nf-core/seqkit/sort/tests/tags.yml @@ -0,0 +1,2 @@ +seqkit/sort: + - "modules/nf-core/seqkit/sort/**" diff --git a/modules/nf-core/tidk/explore/environment.yml b/modules/nf-core/tidk/explore/environment.yml new file mode 100644 index 00000000..4fe55407 --- /dev/null +++ b/modules/nf-core/tidk/explore/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "tidk_explore" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::tidk=0.2.41" diff --git a/modules/nf-core/tidk/explore/main.nf b/modules/nf-core/tidk/explore/main.nf new file mode 100644 index 00000000..3de67531 --- /dev/null +++ b/modules/nf-core/tidk/explore/main.nf @@ -0,0 +1,57 @@ +process TIDK_EXPLORE { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/tidk:0.2.41--hdbdd923_0': + 'biocontainers/tidk:0.2.41--hdbdd923_0' }" + + input: + tuple val(meta), path(fasta) + + output: + tuple val(meta), path("*.tidk.explore.tsv") , emit: explore_tsv + tuple val(meta), path("*.top.sequence.txt") , emit: top_sequence, optional: true + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + tidk \\ + explore \\ + $args \\ + $fasta \\ + > ${prefix}.tidk.explore.tsv + + [[ \$(cat ${prefix}.tidk.explore.tsv | wc -l) -gt 1 ]] \\ + && cat \\ + ${prefix}.tidk.explore.tsv \\ + | sed -n 2p \\ + | awk '{print \$1;}' \\ + > ${prefix}.top.sequence.txt \\ + || echo "No sequence identified" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tidk: \$(tidk --version | sed 's/tidk //') + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.tidk.explore.tsv + touch ${prefix}.top.sequence.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tidk: \$(tidk --version | sed 's/tidk //') + END_VERSIONS + """ +} diff --git a/modules/nf-core/tidk/explore/meta.yml b/modules/nf-core/tidk/explore/meta.yml new file mode 100644 index 00000000..582aaf56 --- /dev/null +++ b/modules/nf-core/tidk/explore/meta.yml @@ -0,0 +1,52 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "tidk_explore" +description: | + `tidk explore` attempts to find the simple telomeric repeat unit in the genome provided. + It will report this repeat in its canonical form (e.g. TTAGG -> AACCT). +keywords: + - genomics + - telomere + - search +tools: + - "tidk": + description: tidk is a toolkit to identify and visualise telomeric repeats in genomes + homepage: "https://github.com/tolkit/telomeric-identifier" + documentation: "https://github.com/tolkit/telomeric-identifier" + tool_dev_url: "https://github.com/tolkit/telomeric-identifier" + doi: "10.5281/zenodo.10091385" + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fasta: + type: file + description: The input fasta file + pattern: "*.{fsa,fa,fasta}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - explore_tsv: + type: file + description: Telomeres and their frequencies in TSV format + pattern: "*.tidk.explore.tsv" + - top_sequence: + type: file + description: | + The most frequent telomere sequence if one or more + sequences are identified by the toolkit + pattern: "*.top.sequence.txt" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/nf-core/tidk/explore/tests/main.nf.test b/modules/nf-core/tidk/explore/tests/main.nf.test new file mode 100644 index 00000000..a04fee61 --- /dev/null +++ b/modules/nf-core/tidk/explore/tests/main.nf.test @@ -0,0 +1,86 @@ +nextflow_process { + + name "Test Process TIDK_EXPLORE" + script "../main.nf" + process "TIDK_EXPLORE" + config "./nextflow.config" + + tag "modules" + tag "modules_nfcore" + tag "tidk" + tag "tidk/explore" + + test("homo_sapiens-genome_21_fasta-success") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("homo_sapiens-genome_fasta-no_top_sequence") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.explore_tsv).match("no_top_sequence_explore_tsv") }, + { assert process.out.top_sequence == [] }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("stub") { + + options '-stub' + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert process.out.explore_tsv != null }, + { assert process.out.top_sequence != null }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + +} diff --git a/modules/nf-core/tidk/explore/tests/main.nf.test.snap b/modules/nf-core/tidk/explore/tests/main.nf.test.snap new file mode 100644 index 00000000..c46d31c1 --- /dev/null +++ b/modules/nf-core/tidk/explore/tests/main.nf.test.snap @@ -0,0 +1,68 @@ +{ + "homo_sapiens-genome_21_fasta-success": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.tidk.explore.tsv:md5,89de91ef36eb0925aefca61757f5275f" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.top.sequence.txt:md5,bd63900958df06516e45b887072d788f" + ] + ], + "2": [ + "versions.yml:md5,8de27958aee1d1fc9075e8046114bdb5" + ], + "explore_tsv": [ + [ + { + "id": "test" + }, + "test.tidk.explore.tsv:md5,89de91ef36eb0925aefca61757f5275f" + ] + ], + "top_sequence": [ + [ + { + "id": "test" + }, + "test.top.sequence.txt:md5,bd63900958df06516e45b887072d788f" + ] + ], + "versions": [ + "versions.yml:md5,8de27958aee1d1fc9075e8046114bdb5" + ] + } + ], + "timestamp": "2023-12-05T14:13:24.14906" + }, + "no_top_sequence_explore_tsv": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.tidk.explore.tsv:md5,27b8513be5478ea2b846a407b3639c01" + ] + ] + ], + "timestamp": "2023-12-05T14:16:18.982423" + }, + "versions": { + "content": [ + [ + "versions.yml:md5,8de27958aee1d1fc9075e8046114bdb5" + ] + ], + "timestamp": "2023-12-05T14:13:24.198311" + } +} \ No newline at end of file diff --git a/modules/nf-core/tidk/explore/tests/nextflow.config b/modules/nf-core/tidk/explore/tests/nextflow.config new file mode 100644 index 00000000..d8f33fe0 --- /dev/null +++ b/modules/nf-core/tidk/explore/tests/nextflow.config @@ -0,0 +1,3 @@ +process { + ext.args = '--minimum 5 --maximum 30' +} diff --git a/modules/nf-core/tidk/explore/tests/tags.yml b/modules/nf-core/tidk/explore/tests/tags.yml new file mode 100644 index 00000000..af666655 --- /dev/null +++ b/modules/nf-core/tidk/explore/tests/tags.yml @@ -0,0 +1,2 @@ +tidk/explore: + - "modules/nf-core/tidk/explore/**" diff --git a/modules/nf-core/tidk/plot/environment.yml b/modules/nf-core/tidk/plot/environment.yml new file mode 100644 index 00000000..ed1706e3 --- /dev/null +++ b/modules/nf-core/tidk/plot/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "tidk_plot" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::tidk=0.2.41" diff --git a/modules/nf-core/tidk/plot/main.nf b/modules/nf-core/tidk/plot/main.nf new file mode 100644 index 00000000..ef56f567 --- /dev/null +++ b/modules/nf-core/tidk/plot/main.nf @@ -0,0 +1,47 @@ +process TIDK_PLOT { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/tidk:0.2.41--hdbdd923_0': + 'biocontainers/tidk:0.2.41--hdbdd923_0' }" + + input: + tuple val(meta), path(tsv) + + output: + tuple val(meta), path("*.svg"), emit: svg + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + tidk \\ + plot \\ + --output $prefix \\ + $args \\ + --tsv "$tsv" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tidk: \$(tidk --version | sed 's/tidk //') + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.svg + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tidk: \$(tidk --version | sed 's/tidk //') + END_VERSIONS + """ +} diff --git a/modules/nf-core/tidk/plot/meta.yml b/modules/nf-core/tidk/plot/meta.yml new file mode 100644 index 00000000..451195c8 --- /dev/null +++ b/modules/nf-core/tidk/plot/meta.yml @@ -0,0 +1,47 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "tidk_plot" +description: | + Plots telomeric repeat frequency against sliding window location + using data produced by `tidk/search` +keywords: + - genomics + - telomere + - search + - plot +tools: + - "tidk": + description: tidk is a toolkit to identify and visualise telomeric repeats in genomes + homepage: "https://github.com/tolkit/telomeric-identifier" + documentation: "https://github.com/tolkit/telomeric-identifier" + tool_dev_url: "https://github.com/tolkit/telomeric-identifier" + doi: "10.5281/zenodo.10091385" + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - tsv: + type: file + description: Search results in TSV format from `tidk search` + pattern: "*.tsv" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - svg: + type: file + description: Telomere search plot + pattern: "*.svg" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/nf-core/tidk/plot/tests/main.nf.test b/modules/nf-core/tidk/plot/tests/main.nf.test new file mode 100644 index 00000000..e267c157 --- /dev/null +++ b/modules/nf-core/tidk/plot/tests/main.nf.test @@ -0,0 +1,74 @@ +nextflow_process { + + name "Test Process TIDK_PLOT" + script "../main.nf" + process "TIDK_PLOT" + + tag "modules" + tag "modules_nfcore" + tag "tidk" + tag "tidk/search" + tag "tidk/plot" + + test("homo_sapiens-genome_21_fasta-success") { + + setup { + run("TIDK_SEARCH") { + script "../../../../nf-core/tidk/search" + + process { + """ + input[0] = [ + [ id:'test' ], + file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) + ] + input[1] = 'TTAGGG' + """ + } + } + } + + when { + process { + """ + input[0] = TIDK_SEARCH.out.tsv + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("stub") { + + options '-stub' + + when { + process { + """ + input[0] = [ + [ id:'test' ], + [] + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + +} diff --git a/modules/nf-core/tidk/plot/tests/main.nf.test.snap b/modules/nf-core/tidk/plot/tests/main.nf.test.snap new file mode 100644 index 00000000..5de9533e --- /dev/null +++ b/modules/nf-core/tidk/plot/tests/main.nf.test.snap @@ -0,0 +1,68 @@ +{ + "homo_sapiens-genome_21_fasta-success": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.svg:md5,e76985fdc220867a05967984c2c4675d" + ] + ], + "1": [ + "versions.yml:md5,b850b0d8b9add80d7863cc62e3f32c99" + ], + "svg": [ + [ + { + "id": "test" + }, + "test.svg:md5,e76985fdc220867a05967984c2c4675d" + ] + ], + "versions": [ + "versions.yml:md5,b850b0d8b9add80d7863cc62e3f32c99" + ] + } + ], + "timestamp": "2023-12-05T14:29:24.721706" + }, + "versions": { + "content": [ + [ + "versions.yml:md5,b850b0d8b9add80d7863cc62e3f32c99" + ] + ], + "timestamp": "2023-12-05T14:29:55.412674" + }, + "stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.svg:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + "versions.yml:md5,b850b0d8b9add80d7863cc62e3f32c99" + ], + "svg": [ + [ + { + "id": "test" + }, + "test.svg:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,b850b0d8b9add80d7863cc62e3f32c99" + ] + } + ], + "timestamp": "2023-12-16T18:50:29.344319" + } +} \ No newline at end of file diff --git a/modules/nf-core/tidk/plot/tests/tags.yml b/modules/nf-core/tidk/plot/tests/tags.yml new file mode 100644 index 00000000..91228695 --- /dev/null +++ b/modules/nf-core/tidk/plot/tests/tags.yml @@ -0,0 +1,2 @@ +tidk/plot: + - "modules/nf-core/tidk/plot/**" diff --git a/modules/nf-core/tidk/search/environment.yml b/modules/nf-core/tidk/search/environment.yml new file mode 100644 index 00000000..eaf92acc --- /dev/null +++ b/modules/nf-core/tidk/search/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "tidk_search" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::tidk=0.2.41" diff --git a/modules/nf-core/tidk/search/main.nf b/modules/nf-core/tidk/search/main.nf new file mode 100644 index 00000000..820f8fda --- /dev/null +++ b/modules/nf-core/tidk/search/main.nf @@ -0,0 +1,62 @@ +process TIDK_SEARCH { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/tidk:0.2.41--hdbdd923_0': + 'biocontainers/tidk:0.2.41--hdbdd923_0' }" + + input: + tuple val(meta), path(fasta) + val string + + output: + tuple val(meta), path("*.tsv") , emit: tsv , optional: true + tuple val(meta), path("*.bedgraph") , emit: bedgraph , optional: true + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + tidk \\ + search \\ + --string $string \\ + --output $prefix \\ + --dir tidk \\ + $args \\ + $fasta + + mv \\ + tidk/${prefix}_telomeric_repeat_windows.tsv \\ + ${prefix}.tsv \\ + || echo "TSV file was not produced" + + mv \\ + tidk/${prefix}_telomeric_repeat_windows.bedgraph \\ + ${prefix}.bedgraph \\ + || echo "BEDGRAPH file was not produced" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tidk: \$(tidk --version | sed 's/tidk //') + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def extension = args.contains("--extension bedgraph") ? 'bedgraph' : 'tsv' + """ + touch ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tidk: \$(tidk --version | sed 's/tidk //') + END_VERSIONS + """ +} diff --git a/modules/nf-core/tidk/search/meta.yml b/modules/nf-core/tidk/search/meta.yml new file mode 100644 index 00000000..8ba07350 --- /dev/null +++ b/modules/nf-core/tidk/search/meta.yml @@ -0,0 +1,51 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "tidk_search" +description: Searches a genome for a telomere string such as TTAGGG +keywords: + - genomics + - telomere + - search +tools: + - "tidk": + description: tidk is a toolkit to identify and visualise telomeric repeats in genomes + homepage: "https://github.com/tolkit/telomeric-identifier" + documentation: "https://github.com/tolkit/telomeric-identifier" + tool_dev_url: "https://github.com/tolkit/telomeric-identifier" + doi: "10.5281/zenodo.10091385" + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fasta: + type: file + description: The input fasta file + pattern: "*.{fsa,fa,fasta}" + - string: + type: string + description: Search string such as TTAGGG +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - tsv: + type: file + description: Search results in TSV format + pattern: "*.tsv" + - bedgraph: + type: file + description: Search results in BEDGRAPH format + pattern: "*.bedgraph" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/nf-core/tidk/search/tests/main.nf.test b/modules/nf-core/tidk/search/tests/main.nf.test new file mode 100644 index 00000000..6dcbe577 --- /dev/null +++ b/modules/nf-core/tidk/search/tests/main.nf.test @@ -0,0 +1,119 @@ +nextflow_process { + + name "Test Process TIDK_SEARCH" + script "../main.nf" + process "TIDK_SEARCH" + + tag "modules" + tag "modules_nfcore" + tag "tidk" + tag "tidk/search" + + test("homo_sapiens-genome_fasta-bedgraph") { + + config "./nextflow.config" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + input[1] = 'TTAGGG' + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.bedgraph).match("bedgraph") }, + { assert snapshot(process.out.versions).match("versions") }, + { assert process.out.tsv == [] } + ) + } + + } + + test("homo_sapiens-genome_fasta-tsv") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + input[1] = 'TTAGGG' + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.tsv).match("tsv") }, + { assert snapshot(process.out.versions).match("versions") }, + { assert process.out.bedgraph == [] } + ) + } + + } + + test("stub-bedgraph") { + + options '-stub' + config "./nextflow.config" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + input[1] = 'TTAGGG' + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert process.out.bedgraph != null }, + { assert process.out.tsv == [] }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("stub-tsv") { + + options '-stub' + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + input[1] = 'TTAGGG' + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert process.out.bedgraph == [] }, + { assert process.out.tsv != null }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + +} diff --git a/modules/nf-core/tidk/search/tests/main.nf.test.snap b/modules/nf-core/tidk/search/tests/main.nf.test.snap new file mode 100644 index 00000000..15aedd25 --- /dev/null +++ b/modules/nf-core/tidk/search/tests/main.nf.test.snap @@ -0,0 +1,36 @@ +{ + "bedgraph": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.bedgraph:md5,8277cc74bf083f51584ba6d2b8a8013f" + ] + ] + ], + "timestamp": "2023-12-05T09:53:52.893139" + }, + "versions": { + "content": [ + [ + "versions.yml:md5,6baeeb0e50c9bea6975173a329179f8e" + ] + ], + "timestamp": "2023-12-05T09:53:52.89726" + }, + "tsv": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.tsv:md5,5b44a89396f412eb571ea240ef9deedd" + ] + ] + ], + "timestamp": "2023-12-05T09:53:56.455271" + } +} \ No newline at end of file diff --git a/modules/nf-core/tidk/search/tests/nextflow.config b/modules/nf-core/tidk/search/tests/nextflow.config new file mode 100644 index 00000000..5d36acfb --- /dev/null +++ b/modules/nf-core/tidk/search/tests/nextflow.config @@ -0,0 +1,3 @@ +process { + ext.args = '--extension bedgraph' +} diff --git a/modules/nf-core/tidk/search/tests/tags.yml b/modules/nf-core/tidk/search/tests/tags.yml new file mode 100644 index 00000000..6d27fc6d --- /dev/null +++ b/modules/nf-core/tidk/search/tests/tags.yml @@ -0,0 +1,2 @@ +tidk/search: + - "modules/nf-core/tidk/search/**" diff --git a/subworkflows/local/fasta_busco_plot.nf b/subworkflows/local/fasta_busco_plot.nf index 7a8bd8fd..f6cc5dc7 100644 --- a/subworkflows/local/fasta_busco_plot.nf +++ b/subworkflows/local/fasta_busco_plot.nf @@ -9,6 +9,8 @@ workflow FASTA_BUSCO_PLOT { download_path // val; Use [] to use work directory. Useful on AWS main: + ch_versions = Channel.empty() + // MODULE: BUSCO BUSCO( tuple_of_hap_file, @@ -20,13 +22,16 @@ workflow FASTA_BUSCO_PLOT { ch_busco_summaries = BUSCO.out.summary | collect + ch_versions = ch_versions.mix(BUSCO.out.versions.first()) + // MODULE: BUSCO_PLOT BUSCO_PLOT ( ch_busco_summaries ) ch_busco_plot = BUSCO_PLOT.out.png + ch_versions = ch_versions.mix(BUSCO_PLOT.out.versions.first()) emit: summary = BUSCO.out.summary plot = ch_busco_plot - versions = Channel.empty().mix(BUSCO.out.versions.first()) + versions = ch_versions } diff --git a/subworkflows/nf-core/fasta_explore_search_plot_tidk/main.nf b/subworkflows/nf-core/fasta_explore_search_plot_tidk/main.nf new file mode 100644 index 00000000..091f0c2c --- /dev/null +++ b/subworkflows/nf-core/fasta_explore_search_plot_tidk/main.nf @@ -0,0 +1,90 @@ +include { SEQKIT_SEQ as FILTER_BY_LENGTH } from '../../../modules/nf-core/seqkit/seq' +include { SEQKIT_SORT as SORT_BY_LENGTH } from '../../../modules/nf-core/seqkit/sort' +include { TIDK_EXPLORE } from '../../../modules/nf-core/tidk/explore' +include { TIDK_SEARCH as TIDK_SEARCH_APRIORI } from '../../../modules/nf-core/tidk/search' +include { TIDK_SEARCH as TIDK_SEARCH_APOSTERIORI } from '../../../modules/nf-core/tidk/search' +include { TIDK_PLOT as TIDK_PLOT_APRIORI } from '../../../modules/nf-core/tidk/plot' +include { TIDK_PLOT as TIDK_PLOT_APOSTERIORI } from '../../../modules/nf-core/tidk/plot' + + +workflow FASTA_EXPLORE_SEARCH_PLOT_TIDK { + + take: + ch_fasta // channel: [ val(meta), [ fasta ] ] + ch_apriori_sequence // channel: [ val(meta), val(sequence) ]; Optional: Set to [] if not needed + // val(meta) from ch_fasta and ch_apriori_sequence are only required to have + // the same `id` + + main: + ch_versions = Channel.empty() + + // MODULE: SEQKIT_SEQ as FILTER_BY_LENGTH + FILTER_BY_LENGTH ( ch_fasta ) + + ch_filtered_fasta = FILTER_BY_LENGTH.out.fastx + ch_versions = ch_versions.mix(FILTER_BY_LENGTH.out.versions.first()) + + // MODULE: SEQKIT_SORT as SORT_BY_LENGTH + SORT_BY_LENGTH ( ch_filtered_fasta ) + + ch_sorted_fasta = SORT_BY_LENGTH.out.fastx + ch_versions = ch_versions.mix(SORT_BY_LENGTH.out.versions.first()) + + // TIDK_EXPLORE + TIDK_EXPLORE ( ch_filtered_fasta ) + + ch_top_sequence = TIDK_EXPLORE.out.top_sequence + ch_versions = ch_versions.mix(TIDK_EXPLORE.out.versions.first()) + + // TIDK_SEARCH as TIDK_SEARCH_APRIORI + ch_apriori_inputs = ch_sorted_fasta + | map { meta, fasta -> [ meta.id, meta, fasta ] } + | join( + ( ch_apriori_sequence ?: Channel.empty() ) + | map { meta, seq -> [ meta.id, seq ] } + ) + | map { id, meta, fasta, seq -> [ meta, fasta, seq ] } + + TIDK_SEARCH_APRIORI ( + ch_apriori_inputs.map { meta, fasta, seq -> [ meta, fasta ] }, + ch_apriori_inputs.map { meta, fasta, seq -> seq } + ) + + ch_apriori_tsv = TIDK_SEARCH_APRIORI.out.tsv + ch_versions = ch_versions.mix(TIDK_SEARCH_APRIORI.out.versions.first()) + + // TIDK_SEARCH as TIDK_SEARCH_APOSTERIORI + ch_aposteriori_inputs = ch_sorted_fasta + | join(ch_top_sequence) + | map { meta, fasta, txt -> + [ meta, fasta, txt.getText().strip() ] + } + + TIDK_SEARCH_APOSTERIORI ( + ch_aposteriori_inputs.map { meta, fasta, seq -> [ meta, fasta ] }, + ch_aposteriori_inputs.map { meta, fasta, seq -> seq } + ) + + ch_aposteriori_tsv = TIDK_SEARCH_APOSTERIORI.out.tsv + ch_versions = ch_versions.mix(TIDK_SEARCH_APOSTERIORI.out.versions.first()) + + // TIDK_PLOT as TIDK_PLOT_APRIORI + TIDK_PLOT_APRIORI ( ch_apriori_tsv ) + + ch_apriori_svg = TIDK_PLOT_APRIORI.out.svg + ch_versions = ch_versions.mix(TIDK_PLOT_APRIORI.out.versions.first()) + + // TIDK_PLOT as TIDK_PLOT_APOSTERIORI + TIDK_PLOT_APOSTERIORI ( ch_aposteriori_tsv ) + + ch_aposteriori_svg = TIDK_PLOT_APOSTERIORI.out.svg + ch_versions = ch_versions.mix(TIDK_PLOT_APOSTERIORI.out.versions.first()) + + emit: + apriori_tsv = ch_apriori_tsv // channel: [ val(meta), tsv ] + apriori_svg = ch_apriori_svg // channel: [ val(meta), svg ] + aposteriori_sequence = ch_top_sequence // channel: [ val(meta), txt ] + aposteriori_tsv = ch_aposteriori_tsv // channel: [ val(meta), tsv ] + aposteriori_svg = ch_aposteriori_svg // channel: [ val(meta), svg ] + versions = ch_versions // channel: [ versions.yml ] +} diff --git a/subworkflows/nf-core/fasta_explore_search_plot_tidk/meta.yml b/subworkflows/nf-core/fasta_explore_search_plot_tidk/meta.yml new file mode 100644 index 00000000..80bd15fd --- /dev/null +++ b/subworkflows/nf-core/fasta_explore_search_plot_tidk/meta.yml @@ -0,0 +1,74 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "fasta_explore_search_plot_tidk" +description: | + Uses Telomere Identification toolKit (TIDK) to identify the frequency of telomeric repeats + along a sliding window for each sequence in the input fasta file. Results are presented in + TSV and SVG formats. The user can specify an a priori sequence for identification. + Possible a posteriori sequences are also explored and the most frequent sequence is + used for identification similar to the a priori sequence. seqkit/seq and seqkit/sort modules are + also included to filter out small sequences and sort sequences by length. +keywords: + - genomics + - telomere + - repeat + - search + - plot +components: + - seqkit/seq + - seqkit/sort + - tidk/explore + - tidk/plot + - tidk/search +input: + - ch_fasta: + type: file + description: | + Input assembly + Structure: [ val(meta), path(fasta) ] + pattern: "*.{fsa/fa/fasta}" + - ch_apriori_sequence: + type: string + description: | + A priori sequence + Structure: [ val(meta), val(sequence) ] +output: + - apriori_tsv: + type: file + description: | + Frequency table for the identification of the a priori sequence + Structure: [ val(meta), path(tsv) ] + pattern: "*.tsv" + - apriori_svg: + type: file + description: | + Frequency graph for the identification of the a priori sequence + Structure: [ val(meta), path(svg) ] + pattern: "*.svg" + - aposteriori_sequence: + type: file + description: | + The most frequent a posteriori sequence + Structure: [ val(meta), path(txt) ] + pattern: "*.txt" + - aposteriori_tsv: + type: file + description: | + Frequency table for the identification of the a aposteriori sequence + Structure: [ val(meta), path(tsv) ] + pattern: "*.tsv" + - aposteriori_svg: + type: file + description: | + Frequency graph for the identification of the a aposteriori sequence + Structure: [ val(meta), path(svg) ] + pattern: "*.svg" + - versions: + type: file + description: | + File containing software versions + Structure: [ path(versions.yml) ] + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test b/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test new file mode 100644 index 00000000..6bc13bac --- /dev/null +++ b/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test @@ -0,0 +1,119 @@ +nextflow_workflow { + + name "Test Workflow FASTA_EXPLORE_SEARCH_PLOT_TIDK" + script "../main.nf" + workflow "FASTA_EXPLORE_SEARCH_PLOT_TIDK" + config './nextflow.config' + + tag "subworkflows" + tag "subworkflows_nfcore" + tag "subworkflows/fasta_explore_search_plot_tidk" + tag "fasta_explore_search_plot_tidk" + tag "seqkit/seq" + tag "seqkit/sort" + tag "tidk/explore" + tag "tidk/search" + tag "tidk/plot" + + test("homo_sapiens-genome_fasta-genome_21_fasta") { + + when { + workflow { + """ + input[0] = Channel.of( + [ + [ id:'test' ], + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ], + [ + [ id:'test2' ], + file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) + ] + ) + input[1] = Channel.of( + [ + [ id:'test' ], + 'TTAGGG' + ], + [ + [ id:'test2' ], + 'TTAGGG' + ] + ) + """ + } + } + + then { + assertAll( + { assert workflow.success}, + { assert snapshot(workflow.out).match()} + ) + } + } + + test("homo_sapiens-genome_fasta-genome_21_fasta-partial_apriori-stub") { + + options '-stub' + + when { + workflow { + """ + input[0] = Channel.of( + [ + [ id:'test' ], + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ], + [ + [ id:'test2' ], + file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) + ] + ) + input[1] = Channel.of( + [ + [ id:'test' ], + 'TTAGGG' + ] + ) + """ + } + } + + then { + assertAll( + { assert workflow.success}, + { assert snapshot(workflow.out).match()} + ) + } + } + + test("homo_sapiens-genome_fasta-genome_21_fasta-no_apriori-stub") { + + options '-stub' + + when { + workflow { + """ + input[0] = Channel.of( + [ + [ id:'test' ], + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ], + [ + [ id:'test2' ], + file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) + ] + ) + input[1] = [] + """ + } + } + + then { + assertAll( + { assert workflow.success}, + { assert snapshot(workflow.out).match()} + ) + } + } +} diff --git a/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test.snap b/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test.snap new file mode 100644 index 00000000..6e4e99c6 --- /dev/null +++ b/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/main.nf.test.snap @@ -0,0 +1,389 @@ +{ + "homo_sapiens-genome_fasta-genome_21_fasta": { + "content": [ + { + "0": [ + [ + { + "id": "test2" + }, + "test2.tsv:md5,4a42ed016c022a8238739e4acddf649c" + ], + [ + { + "id": "test" + }, + "test.tsv:md5,5b44a89396f412eb571ea240ef9deedd" + ] + ], + "1": [ + [ + { + "id": "test2" + }, + "test2.svg:md5,e76985fdc220867a05967984c2c4675d" + ], + [ + { + "id": "test" + }, + "test.svg:md5,d1bca1077cd52ad8c5126a4c9614d8f7" + ] + ], + "2": [ + [ + { + "id": "test2" + }, + "test2.top.sequence.txt:md5,bd63900958df06516e45b887072d788f" + ] + ], + "3": [ + [ + { + "id": "test2" + }, + "test2.tsv:md5,43f358de532d30e02e65339db07e4e00" + ] + ], + "4": [ + [ + { + "id": "test2" + }, + "test2.svg:md5,f0a6d4bb1464125bcbb6b54a471c1e3c" + ] + ], + "5": [ + "versions.yml:md5,02d48eb43c3882d9832c88b9e080b420", + "versions.yml:md5,506585e66b23f17620bf582ef60af56d", + "versions.yml:md5,5f9958ea613eceae4b09bb42ecaac656", + "versions.yml:md5,6713d71b01fe0402e5d32f1a5a181e0f", + "versions.yml:md5,83a6abbf9b68ec1e6152c5b6eb12f0d7", + "versions.yml:md5,b900c2a21c17ac2d16e33641e64fc7c1", + "versions.yml:md5,d0a1f1950f8dc5eb106b04a9364c8f04" + ], + "aposteriori_sequence": [ + [ + { + "id": "test2" + }, + "test2.top.sequence.txt:md5,bd63900958df06516e45b887072d788f" + ] + ], + "aposteriori_svg": [ + [ + { + "id": "test2" + }, + "test2.svg:md5,f0a6d4bb1464125bcbb6b54a471c1e3c" + ] + ], + "aposteriori_tsv": [ + [ + { + "id": "test2" + }, + "test2.tsv:md5,43f358de532d30e02e65339db07e4e00" + ] + ], + "apriori_svg": [ + [ + { + "id": "test2" + }, + "test2.svg:md5,e76985fdc220867a05967984c2c4675d" + ], + [ + { + "id": "test" + }, + "test.svg:md5,d1bca1077cd52ad8c5126a4c9614d8f7" + ] + ], + "apriori_tsv": [ + [ + { + "id": "test2" + }, + "test2.tsv:md5,4a42ed016c022a8238739e4acddf649c" + ], + [ + { + "id": "test" + }, + "test.tsv:md5,5b44a89396f412eb571ea240ef9deedd" + ] + ], + "versions": [ + "versions.yml:md5,02d48eb43c3882d9832c88b9e080b420", + "versions.yml:md5,506585e66b23f17620bf582ef60af56d", + "versions.yml:md5,5f9958ea613eceae4b09bb42ecaac656", + "versions.yml:md5,6713d71b01fe0402e5d32f1a5a181e0f", + "versions.yml:md5,83a6abbf9b68ec1e6152c5b6eb12f0d7", + "versions.yml:md5,b900c2a21c17ac2d16e33641e64fc7c1", + "versions.yml:md5,d0a1f1950f8dc5eb106b04a9364c8f04" + ] + } + ], + "timestamp": "2023-12-12T15:33:35.106116" + }, + "homo_sapiens-genome_fasta-genome_21_fasta-no_apriori-stub": { + "content": [ + { + "0": [ + + ], + "1": [ + + ], + "2": [ + [ + { + "id": "test2" + }, + "test2.top.sequence.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + [ + { + "id": "test" + }, + "test.top.sequence.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "3": [ + [ + { + "id": "test2" + }, + "test2.tsv:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + [ + { + "id": "test" + }, + "test.tsv:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "4": [ + [ + { + "id": "test2" + }, + "test2.svg:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + [ + { + "id": "test" + }, + "test.svg:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "5": [ + "versions.yml:md5,02d48eb43c3882d9832c88b9e080b420", + "versions.yml:md5,506585e66b23f17620bf582ef60af56d", + "versions.yml:md5,5f9958ea613eceae4b09bb42ecaac656", + "versions.yml:md5,6713d71b01fe0402e5d32f1a5a181e0f", + "versions.yml:md5,d0a1f1950f8dc5eb106b04a9364c8f04" + ], + "aposteriori_sequence": [ + [ + { + "id": "test2" + }, + "test2.top.sequence.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + [ + { + "id": "test" + }, + "test.top.sequence.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "aposteriori_svg": [ + [ + { + "id": "test2" + }, + "test2.svg:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + [ + { + "id": "test" + }, + "test.svg:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "aposteriori_tsv": [ + [ + { + "id": "test2" + }, + "test2.tsv:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + [ + { + "id": "test" + }, + "test.tsv:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "apriori_svg": [ + + ], + "apriori_tsv": [ + + ], + "versions": [ + "versions.yml:md5,02d48eb43c3882d9832c88b9e080b420", + "versions.yml:md5,506585e66b23f17620bf582ef60af56d", + "versions.yml:md5,5f9958ea613eceae4b09bb42ecaac656", + "versions.yml:md5,6713d71b01fe0402e5d32f1a5a181e0f", + "versions.yml:md5,d0a1f1950f8dc5eb106b04a9364c8f04" + ] + } + ], + "timestamp": "2023-12-21T11:15:42.277945" + }, + "homo_sapiens-genome_fasta-genome_21_fasta-partial_apriori-stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.tsv:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.svg:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + [ + { + "id": "test2" + }, + "test2.top.sequence.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + [ + { + "id": "test" + }, + "test.top.sequence.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "3": [ + [ + { + "id": "test2" + }, + "test2.tsv:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + [ + { + "id": "test" + }, + "test.tsv:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "4": [ + [ + { + "id": "test2" + }, + "test2.svg:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + [ + { + "id": "test" + }, + "test.svg:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "5": [ + "versions.yml:md5,02d48eb43c3882d9832c88b9e080b420", + "versions.yml:md5,506585e66b23f17620bf582ef60af56d", + "versions.yml:md5,5f9958ea613eceae4b09bb42ecaac656", + "versions.yml:md5,6713d71b01fe0402e5d32f1a5a181e0f", + "versions.yml:md5,83a6abbf9b68ec1e6152c5b6eb12f0d7", + "versions.yml:md5,b900c2a21c17ac2d16e33641e64fc7c1", + "versions.yml:md5,d0a1f1950f8dc5eb106b04a9364c8f04" + ], + "aposteriori_sequence": [ + [ + { + "id": "test2" + }, + "test2.top.sequence.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + [ + { + "id": "test" + }, + "test.top.sequence.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "aposteriori_svg": [ + [ + { + "id": "test2" + }, + "test2.svg:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + [ + { + "id": "test" + }, + "test.svg:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "aposteriori_tsv": [ + [ + { + "id": "test2" + }, + "test2.tsv:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + [ + { + "id": "test" + }, + "test.tsv:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "apriori_svg": [ + [ + { + "id": "test" + }, + "test.svg:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "apriori_tsv": [ + [ + { + "id": "test" + }, + "test.tsv:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,02d48eb43c3882d9832c88b9e080b420", + "versions.yml:md5,506585e66b23f17620bf582ef60af56d", + "versions.yml:md5,5f9958ea613eceae4b09bb42ecaac656", + "versions.yml:md5,6713d71b01fe0402e5d32f1a5a181e0f", + "versions.yml:md5,83a6abbf9b68ec1e6152c5b6eb12f0d7", + "versions.yml:md5,b900c2a21c17ac2d16e33641e64fc7c1", + "versions.yml:md5,d0a1f1950f8dc5eb106b04a9364c8f04" + ] + } + ], + "timestamp": "2023-12-21T11:15:25.633714" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/nextflow.config b/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/nextflow.config new file mode 100644 index 00000000..22d4fde0 --- /dev/null +++ b/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/nextflow.config @@ -0,0 +1,19 @@ +process { + + withName: FILTER_BY_LENGTH { + ext.prefix = { "${meta.id}.filtered" } + } + + withName: SORT_BY_LENGTH { + ext.args = '--quiet --reverse --by-length' + ext.prefix = { "${meta.id}.sorted" } + } + + withName: TIDK_EXPLORE { + ext.args = '--minimum 5 --maximum 30' + } + + withName: 'TIDK_SEARCH_*' { + ext.args = '--extension tsv' + } +} diff --git a/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/tags.yml b/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/tags.yml new file mode 100644 index 00000000..d2d150d7 --- /dev/null +++ b/subworkflows/nf-core/fasta_explore_search_plot_tidk/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/fasta_explore_search_plot_tidk: + - subworkflows/nf-core/fasta_explore_search_plot_tidk/** diff --git a/tests/stub/stub.config b/tests/stub/stub.config index 371b42ac..6daf7008 100644 --- a/tests/stub/stub.config +++ b/tests/stub/stub.config @@ -13,6 +13,8 @@ params { busco_skip = false busco_lineage_datasets = 'fungi_odb10 hypocreales_odb10' + tidk_skip = false + // Limit resources so that this can run on GitHub Actions max_cpus = 2 max_memory = '6.GB' diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index 4758b636..cef4bb86 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -47,6 +47,7 @@ include { FASTA_BUSCO_PLOT } from '../subworkflows/local/fasta_ include { GUNZIP as GUNZIP_FASTA } from '../modules/nf-core/gunzip/main' include { GUNZIP as GUNZIP_GFF3 } from '../modules/nf-core/gunzip/main' include { FASTAVALIDATOR } from '../modules/nf-core/fastavalidator/main' +include { FASTA_EXPLORE_SEARCH_PLOT_TIDK } from '../subworkflows/nf-core/fasta_explore_search_plot_tidk/main' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' @@ -277,6 +278,31 @@ workflow ASSEMBLYQC { ch_busco_plot = FASTA_BUSCO_PLOT.out.plot ch_versions = ch_versions.mix(FASTA_BUSCO_PLOT.out.versions) + // SUBWORKFLOW: FASTA_EXPLORE_SEARCH_PLOT_TIDK + ch_tidk_inputs = params.tidk_skip + ? Channel.empty() + : ch_clean_assembly + | map { tag, fa -> [ [ id: tag ], fa ] } + | combine( + Channel.of(params.tidk_repeat_seq) + ) + + FASTA_EXPLORE_SEARCH_PLOT_TIDK( + ch_tidk_inputs.map { meta, fa, seq -> [ meta, fa ] }, + ch_tidk_inputs.map { meta, fa, seq -> [ meta, seq ] } + ) + + ch_tidk_outputs = FASTA_EXPLORE_SEARCH_PLOT_TIDK.out.apriori_svg + | mix(FASTA_EXPLORE_SEARCH_PLOT_TIDK.out.aposteriori_svg) + | mix(FASTA_EXPLORE_SEARCH_PLOT_TIDK.out.aposteriori_sequence) + | map { meta, file -> file } + | mix( + Channel.of("$params.tidk_repeat_seq") + | collectFile(name: 'a_priori.sequence', newLine: true) + ) + + ch_versions = ch_versions.mix(FASTA_EXPLORE_SEARCH_PLOT_TIDK.out.versions) + // MODULE: CUSTOM_DUMPSOFTWAREVERSIONS CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') From d6ba080748f91abd1a535c90de3e4065e1a86e93 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 22 Feb 2024 21:24:10 +1300 Subject: [PATCH 24/65] Added fasta_ltrretriever_lai --- conf/modules.config | 8 +- modules.json | 40 ++++ modules/pfr/cat/cat/environment.yml | 7 + modules/pfr/cat/cat/main.nf | 79 ++++++++ modules/pfr/cat/cat/meta.yml | 36 ++++ modules/pfr/cat/cat/tests/main.nf.test | 177 +++++++++++++++++ modules/pfr/cat/cat/tests/main.nf.test.snap | 145 ++++++++++++++ .../cat/tests/nextflow_unzipped_zipped.config | 6 + .../cat/tests/nextflow_zipped_unzipped.config | 8 + modules/pfr/cat/cat/tests/tags.yml | 2 + .../pfr/custom/restoregffids/environment.yml | 9 + modules/pfr/custom/restoregffids/main.nf | 35 ++++ modules/pfr/custom/restoregffids/meta.yml | 58 ++++++ .../templates/restore_gff_ids.py | 47 +++++ .../custom/restoregffids/tests/main.nf.test | 63 ++++++ .../restoregffids/tests/main.nf.test.snap | 41 ++++ .../pfr/custom/restoregffids/tests/tags.yml | 2 + .../custom/shortenfastaids/environment.yml | 11 ++ modules/pfr/custom/shortenfastaids/main.nf | 34 ++++ modules/pfr/custom/shortenfastaids/meta.yml | 58 ++++++ .../templates/shorten_fasta_ids.py | 181 ++++++++++++++++++ .../custom/shortenfastaids/tests/main.nf.test | 131 +++++++++++++ .../shortenfastaids/tests/main.nf.test.snap | 170 ++++++++++++++++ .../pfr/custom/shortenfastaids/tests/tags.yml | 2 + modules/pfr/ltrfinder/environment.yml | 9 + modules/pfr/ltrfinder/main.nf | 53 +++++ modules/pfr/ltrfinder/meta.yml | 60 ++++++ modules/pfr/ltrfinder/tests/main.nf.test | 72 +++++++ modules/pfr/ltrfinder/tests/main.nf.test.snap | 120 ++++++++++++ modules/pfr/ltrfinder/tests/tags.yml | 2 + modules/pfr/ltrharvest/environment.yml | 9 + modules/pfr/ltrharvest/main.nf | 56 ++++++ modules/pfr/ltrharvest/meta.yml | 60 ++++++ modules/pfr/ltrharvest/tests/main.nf.test | 60 ++++++ .../pfr/ltrharvest/tests/main.nf.test.snap | 88 +++++++++ modules/pfr/ltrharvest/tests/tags.yml | 2 + modules/pfr/ltrretriever/lai/environment.yml | 9 + modules/pfr/ltrretriever/lai/main.nf | 71 +++++++ modules/pfr/ltrretriever/lai/meta.yml | 70 +++++++ .../pfr/ltrretriever/lai/tests/main.nf.test | 166 ++++++++++++++++ .../ltrretriever/lai/tests/main.nf.test.snap | 100 ++++++++++ .../ltrretriever/lai/tests/nextflow.config | 15 ++ modules/pfr/ltrretriever/lai/tests/tags.yml | 2 + .../ltrretriever/ltrretriever/environment.yml | 9 + modules/pfr/ltrretriever/ltrretriever/main.nf | 77 ++++++++ .../pfr/ltrretriever/ltrretriever/meta.yml | 83 ++++++++ .../ltrretriever/tests/main.nf.test | 133 +++++++++++++ .../ltrretriever/tests/main.nf.test.snap | 169 ++++++++++++++++ .../ltrretriever/tests/nextflow.config | 21 ++ .../ltrretriever/ltrretriever/tests/tags.yml | 2 + .../pfr/fasta_ltrretriever_lai/main.nf | 114 +++++++++++ .../pfr/fasta_ltrretriever_lai/meta.yml | 78 ++++++++ .../fasta_ltrretriever_lai/tests/main.nf.test | 59 ++++++ .../tests/nextflow.config | 14 ++ .../pfr/fasta_ltrretriever_lai/tests/tags.yml | 2 + tests/stub/assemblysheet.csv | 2 +- tests/stub/stub.config | 2 + workflows/assemblyqc.nf | 36 +++- 58 files changed, 3168 insertions(+), 7 deletions(-) create mode 100644 modules/pfr/cat/cat/environment.yml create mode 100644 modules/pfr/cat/cat/main.nf create mode 100644 modules/pfr/cat/cat/meta.yml create mode 100644 modules/pfr/cat/cat/tests/main.nf.test create mode 100644 modules/pfr/cat/cat/tests/main.nf.test.snap create mode 100644 modules/pfr/cat/cat/tests/nextflow_unzipped_zipped.config create mode 100644 modules/pfr/cat/cat/tests/nextflow_zipped_unzipped.config create mode 100644 modules/pfr/cat/cat/tests/tags.yml create mode 100644 modules/pfr/custom/restoregffids/environment.yml create mode 100644 modules/pfr/custom/restoregffids/main.nf create mode 100644 modules/pfr/custom/restoregffids/meta.yml create mode 100755 modules/pfr/custom/restoregffids/templates/restore_gff_ids.py create mode 100644 modules/pfr/custom/restoregffids/tests/main.nf.test create mode 100644 modules/pfr/custom/restoregffids/tests/main.nf.test.snap create mode 100644 modules/pfr/custom/restoregffids/tests/tags.yml create mode 100644 modules/pfr/custom/shortenfastaids/environment.yml create mode 100644 modules/pfr/custom/shortenfastaids/main.nf create mode 100644 modules/pfr/custom/shortenfastaids/meta.yml create mode 100755 modules/pfr/custom/shortenfastaids/templates/shorten_fasta_ids.py create mode 100644 modules/pfr/custom/shortenfastaids/tests/main.nf.test create mode 100644 modules/pfr/custom/shortenfastaids/tests/main.nf.test.snap create mode 100644 modules/pfr/custom/shortenfastaids/tests/tags.yml create mode 100644 modules/pfr/ltrfinder/environment.yml create mode 100644 modules/pfr/ltrfinder/main.nf create mode 100644 modules/pfr/ltrfinder/meta.yml create mode 100644 modules/pfr/ltrfinder/tests/main.nf.test create mode 100644 modules/pfr/ltrfinder/tests/main.nf.test.snap create mode 100644 modules/pfr/ltrfinder/tests/tags.yml create mode 100644 modules/pfr/ltrharvest/environment.yml create mode 100644 modules/pfr/ltrharvest/main.nf create mode 100644 modules/pfr/ltrharvest/meta.yml create mode 100644 modules/pfr/ltrharvest/tests/main.nf.test create mode 100644 modules/pfr/ltrharvest/tests/main.nf.test.snap create mode 100644 modules/pfr/ltrharvest/tests/tags.yml create mode 100644 modules/pfr/ltrretriever/lai/environment.yml create mode 100644 modules/pfr/ltrretriever/lai/main.nf create mode 100644 modules/pfr/ltrretriever/lai/meta.yml create mode 100644 modules/pfr/ltrretriever/lai/tests/main.nf.test create mode 100644 modules/pfr/ltrretriever/lai/tests/main.nf.test.snap create mode 100644 modules/pfr/ltrretriever/lai/tests/nextflow.config create mode 100644 modules/pfr/ltrretriever/lai/tests/tags.yml create mode 100644 modules/pfr/ltrretriever/ltrretriever/environment.yml create mode 100644 modules/pfr/ltrretriever/ltrretriever/main.nf create mode 100644 modules/pfr/ltrretriever/ltrretriever/meta.yml create mode 100644 modules/pfr/ltrretriever/ltrretriever/tests/main.nf.test create mode 100644 modules/pfr/ltrretriever/ltrretriever/tests/main.nf.test.snap create mode 100644 modules/pfr/ltrretriever/ltrretriever/tests/nextflow.config create mode 100644 modules/pfr/ltrretriever/ltrretriever/tests/tags.yml create mode 100644 subworkflows/pfr/fasta_ltrretriever_lai/main.nf create mode 100644 subworkflows/pfr/fasta_ltrretriever_lai/meta.yml create mode 100644 subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test create mode 100644 subworkflows/pfr/fasta_ltrretriever_lai/tests/nextflow.config create mode 100644 subworkflows/pfr/fasta_ltrretriever_lai/tests/tags.yml diff --git a/conf/modules.config b/conf/modules.config index d4d26b4e..a22b25d8 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -184,8 +184,8 @@ process { ] } - withName: EDTA_LTRHARVEST { - ext.prefix = { "${meta.id}_edta_ltrharvest" } + withName: LTRHARVEST { + ext.prefix = { "${meta.id}_ltrharvest" } } withName: LTRFINDER { @@ -196,7 +196,7 @@ process { ext.prefix = { "${meta.id}_ltrharvest_ltrfinder.tabout" } } - withName: LTRRETRIEVER { + withName: LTRRETRIEVER_LTRRETRIEVER { publishDir = [ path: { "${params.outdir}/lai" }, mode: params.publish_dir_mode, @@ -212,7 +212,7 @@ process { ] } - withName: LAI { + withName: LTRRETRIEVER_LAI { publishDir = [ path: { "${params.outdir}/lai" }, mode: params.publish_dir_mode, diff --git a/modules.json b/modules.json index c76c0439..b9e6d4f1 100644 --- a/modules.json +++ b/modules.json @@ -5,11 +5,26 @@ "git@github.com:PlantandFoodResearch/nxf-modules.git": { "modules": { "pfr": { + "cat/cat": { + "branch": "main", + "git_sha": "4b9da80b1e4c16067babd97554bea42d7cd9ca85", + "installed_by": ["fasta_ltrretriever_lai"] + }, "custom/checkgff3fastacorrespondence": { "branch": "main", "git_sha": "1a76f884082c786760559c462063a5d1de94ca83", "installed_by": ["gff3_validate"] }, + "custom/restoregffids": { + "branch": "main", + "git_sha": "e9f6bdd634bdbcd52c5568ba82f16176ec06631f", + "installed_by": ["fasta_ltrretriever_lai"] + }, + "custom/shortenfastaids": { + "branch": "main", + "git_sha": "5e0e41b51d7fc7f68ae43692b6fe19b95d7f3a8c", + "installed_by": ["fasta_ltrretriever_lai"] + }, "gt/gff3": { "branch": "main", "git_sha": "bfa4874d3942bdff70cb8df17322834125cafb28", @@ -24,11 +39,36 @@ "branch": "main", "git_sha": "cb5fb0be78a98fd1e32b7c90d6adf8c3bf44133e", "installed_by": ["modules"] + }, + "ltrfinder": { + "branch": "main", + "git_sha": "835879b8f174bb4d2c5534d0381ffbe62cc1b060", + "installed_by": ["fasta_ltrretriever_lai"] + }, + "ltrharvest": { + "branch": "main", + "git_sha": "835879b8f174bb4d2c5534d0381ffbe62cc1b060", + "installed_by": ["fasta_ltrretriever_lai"] + }, + "ltrretriever/lai": { + "branch": "main", + "git_sha": "154661d7c1769532ff7b5f11259644ec200dd47d", + "installed_by": ["fasta_ltrretriever_lai"] + }, + "ltrretriever/ltrretriever": { + "branch": "main", + "git_sha": "835879b8f174bb4d2c5534d0381ffbe62cc1b060", + "installed_by": ["fasta_ltrretriever_lai"] } } }, "subworkflows": { "pfr": { + "fasta_ltrretriever_lai": { + "branch": "main", + "git_sha": "154661d7c1769532ff7b5f11259644ec200dd47d", + "installed_by": ["subworkflows"] + }, "gff3_validate": { "branch": "main", "git_sha": "f9b96bf8142a01f0649ff90570fb10aa973504b9", diff --git a/modules/pfr/cat/cat/environment.yml b/modules/pfr/cat/cat/environment.yml new file mode 100644 index 00000000..17a04ef2 --- /dev/null +++ b/modules/pfr/cat/cat/environment.yml @@ -0,0 +1,7 @@ +name: cat_cat +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - conda-forge::pigz=2.3.4 diff --git a/modules/pfr/cat/cat/main.nf b/modules/pfr/cat/cat/main.nf new file mode 100644 index 00000000..adbdbd7b --- /dev/null +++ b/modules/pfr/cat/cat/main.nf @@ -0,0 +1,79 @@ +process CAT_CAT { + tag "$meta.id" + label 'process_low' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/pigz:2.3.4' : + 'biocontainers/pigz:2.3.4' }" + + input: + tuple val(meta), path(files_in) + + output: + tuple val(meta), path("${prefix}"), emit: file_out + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def file_list = files_in.collect { it.toString() } + + // choose appropriate concatenation tool depending on input and output format + + // | input | output | command1 | command2 | + // |-----------|------------|----------|----------| + // | gzipped | gzipped | cat | | + // | ungzipped | ungzipped | cat | | + // | gzipped | ungzipped | zcat | | + // | ungzipped | gzipped | cat | pigz | + + // Use input file ending as default + prefix = task.ext.prefix ?: "${meta.id}${getFileSuffix(file_list[0])}" + out_zip = prefix.endsWith('.gz') + in_zip = file_list[0].endsWith('.gz') + command1 = (in_zip && !out_zip) ? 'zcat' : 'cat' + command2 = (!in_zip && out_zip) ? "| pigz -c -p $task.cpus $args2" : '' + if(file_list.contains(prefix.trim())) { + error "The name of the input file can't be the same as for the output prefix in the " + + "module CAT_CAT (currently `$prefix`). Please choose a different one." + } + """ + $command1 \\ + $args \\ + ${file_list.join(' ')} \\ + $command2 \\ + > ${prefix} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' ) + END_VERSIONS + """ + + stub: + def file_list = files_in.collect { it.toString() } + prefix = task.ext.prefix ?: "${meta.id}${file_list[0].substring(file_list[0].lastIndexOf('.'))}" + if(file_list.contains(prefix.trim())) { + error "The name of the input file can't be the same as for the output prefix in the " + + "module CAT_CAT (currently `$prefix`). Please choose a different one." + } + """ + touch $prefix + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' ) + END_VERSIONS + """ +} + +// for .gz files also include the second to last extension if it is present. E.g., .fasta.gz +def getFileSuffix(filename) { + def match = filename =~ /^.*?((\.\w{1,5})?(\.\w{1,5}\.gz$))/ + return match ? match[0][1] : filename.substring(filename.lastIndexOf('.')) +} + diff --git a/modules/pfr/cat/cat/meta.yml b/modules/pfr/cat/cat/meta.yml new file mode 100644 index 00000000..00a8db0b --- /dev/null +++ b/modules/pfr/cat/cat/meta.yml @@ -0,0 +1,36 @@ +name: cat_cat +description: A module for concatenation of gzipped or uncompressed files +keywords: + - concatenate + - gzip + - cat +tools: + - cat: + description: Just concatenation + documentation: https://man7.org/linux/man-pages/man1/cat.1.html + licence: ["GPL-3.0-or-later"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - files_in: + type: file + description: List of compressed / uncompressed files + pattern: "*" +output: + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - file_out: + type: file + description: Concatenated file. Will be gzipped if file_out ends with ".gz" + pattern: "${file_out}" +authors: + - "@erikrikarddaniel" + - "@FriederikeHanssen" +maintainers: + - "@erikrikarddaniel" + - "@FriederikeHanssen" diff --git a/modules/pfr/cat/cat/tests/main.nf.test b/modules/pfr/cat/cat/tests/main.nf.test new file mode 100644 index 00000000..aaae04f9 --- /dev/null +++ b/modules/pfr/cat/cat/tests/main.nf.test @@ -0,0 +1,177 @@ +nextflow_process { + + name "Test Process CAT_CAT" + script "../main.nf" + process "CAT_CAT" + tag "modules" + tag "modules_nfcore" + tag "cat" + tag "cat/cat" + + test("test_cat_name_conflict") { + when { + params { + outdir = "${outputDir}" + } + process { + """ + input[0] = + [ + [ id:'genome', single_end:true ], + [ + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), + file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true) + ] + ] + """ + } + } + then { + assertAll( + { assert !process.success }, + { assert process.stdout.toString().contains("The name of the input file can't be the same as for the output prefix") } + ) + } + } + + test("test_cat_unzipped_unzipped") { + when { + params { + outdir = "${outputDir}" + } + process { + """ + input[0] = + [ + [ id:'test', single_end:true ], + [ + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), + file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true) + ] + ] + """ + } + } + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + + test("test_cat_zipped_zipped") { + when { + params { + outdir = "${outputDir}" + } + process { + """ + input[0] = + [ + [ id:'test', single_end:true ], + [ + file(params.test_data['sarscov2']['genome']['genome_gff3_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['genome']['contigs_genome_maf_gz'], checkIfExists: true) + ] + ] + """ + } + } + then { + def lines = path(process.out.file_out.get(0).get(1)).linesGzip + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("test_cat_zipped_unzipped") { + config './nextflow_zipped_unzipped.config' + + when { + params { + outdir = "${outputDir}" + } + process { + """ + input[0] = + [ + [ id:'test', single_end:true ], + [ + file(params.test_data['sarscov2']['genome']['genome_gff3_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['genome']['contigs_genome_maf_gz'], checkIfExists: true) + ] + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("test_cat_unzipped_zipped") { + config './nextflow_unzipped_zipped.config' + when { + params { + outdir = "${outputDir}" + } + process { + """ + input[0] = + [ + [ id:'test', single_end:true ], + [ + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), + file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true) + ] + ] + """ + } + } + then { + def lines = path(process.out.file_out.get(0).get(1)).linesGzip + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("test_cat_one_file_unzipped_zipped") { + config './nextflow_unzipped_zipped.config' + when { + params { + outdir = "${outputDir}" + } + process { + """ + input[0] = + [ + [ id:'test', single_end:true ], + [ + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + ] + """ + } + } + then { + def lines = path(process.out.file_out.get(0).get(1)).linesGzip + assertAll( + { assert process.success }, + { assert snapshot(lines[0..5]).match("test_cat_one_file_unzipped_zipped_lines") }, + { assert snapshot(lines.size()).match("test_cat_one_file_unzipped_zipped_size")} + ) + } + } +} + diff --git a/modules/pfr/cat/cat/tests/main.nf.test.snap b/modules/pfr/cat/cat/tests/main.nf.test.snap new file mode 100644 index 00000000..0c9bfe8d --- /dev/null +++ b/modules/pfr/cat/cat/tests/main.nf.test.snap @@ -0,0 +1,145 @@ +{ + "test_cat_unzipped_unzipped": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test.fasta:md5,f44b33a0e441ad58b2d3700270e2dbe2" + ] + ], + "1": [ + "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" + ], + "file_out": [ + [ + { + "id": "test", + "single_end": true + }, + "test.fasta:md5,f44b33a0e441ad58b2d3700270e2dbe2" + ] + ], + "versions": [ + "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" + ] + } + ], + "timestamp": "2023-10-16T14:32:18.500464399" + }, + "test_cat_zipped_unzipped": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "cat.txt:md5,c439d3b60e7bc03e8802a451a0d9a5d9" + ] + ], + "1": [ + "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" + ], + "file_out": [ + [ + { + "id": "test", + "single_end": true + }, + "cat.txt:md5,c439d3b60e7bc03e8802a451a0d9a5d9" + ] + ], + "versions": [ + "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" + ] + } + ], + "timestamp": "2023-10-16T14:32:49.642741302" + }, + "test_cat_zipped_zipped": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test.gff3.gz:md5,c439d3b60e7bc03e8802a451a0d9a5d9" + ] + ], + "1": [ + "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" + ], + "file_out": [ + [ + { + "id": "test", + "single_end": true + }, + "test.gff3.gz:md5,c439d3b60e7bc03e8802a451a0d9a5d9" + ] + ], + "versions": [ + "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" + ] + } + ], + "timestamp": "2024-01-12T14:02:02.999254641" + }, + "test_cat_one_file_unzipped_zipped_lines": { + "content": [ + [ + ">MT192765.1 Severe acute respiratory syndrome coronavirus 2 isolate SARS-CoV-2/human/USA/PC00101P/2020, complete genome", + "GTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGT", + "GTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAG", + "TAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGG", + "GTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTT", + "ACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAG" + ] + ], + "timestamp": "2023-10-16T14:33:21.39642399" + }, + "test_cat_unzipped_zipped": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "cat.txt.gz:md5,f44b33a0e441ad58b2d3700270e2dbe2" + ] + ], + "1": [ + "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" + ], + "file_out": [ + [ + { + "id": "test", + "single_end": true + }, + "cat.txt.gz:md5,f44b33a0e441ad58b2d3700270e2dbe2" + ] + ], + "versions": [ + "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" + ] + } + ], + "timestamp": "2024-01-12T14:08:26.948048418" + }, + "test_cat_one_file_unzipped_zipped_size": { + "content": [ + 374 + ], + "timestamp": "2024-01-12T14:10:22.445700266" + } +} diff --git a/modules/pfr/cat/cat/tests/nextflow_unzipped_zipped.config b/modules/pfr/cat/cat/tests/nextflow_unzipped_zipped.config new file mode 100644 index 00000000..ec26b0fd --- /dev/null +++ b/modules/pfr/cat/cat/tests/nextflow_unzipped_zipped.config @@ -0,0 +1,6 @@ + +process { + withName: CAT_CAT { + ext.prefix = 'cat.txt.gz' + } +} diff --git a/modules/pfr/cat/cat/tests/nextflow_zipped_unzipped.config b/modules/pfr/cat/cat/tests/nextflow_zipped_unzipped.config new file mode 100644 index 00000000..fbc79783 --- /dev/null +++ b/modules/pfr/cat/cat/tests/nextflow_zipped_unzipped.config @@ -0,0 +1,8 @@ + +process { + + withName: CAT_CAT { + ext.prefix = 'cat.txt' + } + +} diff --git a/modules/pfr/cat/cat/tests/tags.yml b/modules/pfr/cat/cat/tests/tags.yml new file mode 100644 index 00000000..37b578f5 --- /dev/null +++ b/modules/pfr/cat/cat/tests/tags.yml @@ -0,0 +1,2 @@ +cat/cat: + - modules/nf-core/cat/cat/** diff --git a/modules/pfr/custom/restoregffids/environment.yml b/modules/pfr/custom/restoregffids/environment.yml new file mode 100644 index 00000000..2450c456 --- /dev/null +++ b/modules/pfr/custom/restoregffids/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "custom_restoregffids" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "python=3.10.2" diff --git a/modules/pfr/custom/restoregffids/main.nf b/modules/pfr/custom/restoregffids/main.nf new file mode 100644 index 00000000..14e2c077 --- /dev/null +++ b/modules/pfr/custom/restoregffids/main.nf @@ -0,0 +1,35 @@ +process CUSTOM_RESTOREGFFIDS { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/python:3.10.2': + 'biocontainers/python:3.10.2' }" + + input: + tuple val(meta), path(gff3) + path(ids_tsv) + + output: + tuple val(meta), path("*.restored.ids.gff3") , emit: restored_ids_gff3 + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + prefix = task.ext.prefix ?: "${meta.id}" + template 'restore_gff_ids.py' + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch "${prefix}.restored.ids.gff3" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + python: \$(python --version | cut -d' ' -f2) + END_VERSIONS + """ +} diff --git a/modules/pfr/custom/restoregffids/meta.yml b/modules/pfr/custom/restoregffids/meta.yml new file mode 100644 index 00000000..4e42b829 --- /dev/null +++ b/modules/pfr/custom/restoregffids/meta.yml @@ -0,0 +1,58 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "custom_restoregffids" +description: | + Restores IDs in a gff3 file based on a TSV table + consisting of original (first column) and new IDs (second column). + This module is helpful when some tools like EDTA implicitly shorten + the IDs without producing the ID map, leading to downstream mismatch + in IDs across files. +keywords: + - genome + - gff + - ID + - shorten + - restore +tools: + - "python": + description: | + Python is a programming language that lets you work quickly + and integrate systems more effectively + homepage: "https://www.python.org" + documentation: "https://docs.python.org/3/" + tool_dev_url: "https://github.com/python/cpython" + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - gff3: + type: file + description: Input gff3 file + pattern: "*.{gff,gff3}" + - ids_tsv: + type: file + description: | + A TSV file with original (first column) and new ids (second column) + if id change was required + pattern: "*.tsv" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - restored_ids_gff3: + type: file + description: GFF3 file with restored ids + pattern: "*.restored.ids.gff3" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/pfr/custom/restoregffids/templates/restore_gff_ids.py b/modules/pfr/custom/restoregffids/templates/restore_gff_ids.py new file mode 100755 index 00000000..d0699de0 --- /dev/null +++ b/modules/pfr/custom/restoregffids/templates/restore_gff_ids.py @@ -0,0 +1,47 @@ +#!/usr/bin/env python3 + +from platform import python_version + +ids_tsv = "$ids_tsv" +input_gff3 = "$gff3" +output_prefix = "$prefix" + + +def create_name_mapping_from_tsv(file_path): + dictionary = {} + + with open(file_path, "r") as tsv_file: + for line in tsv_file: + columns = line.strip().split("\\t") + if len(columns) != 2: + raise ValueError(f"{file_path} should be a two column TSV file") + + orig_id, new_id = columns[0], columns[1] + dictionary[new_id] = orig_id + + return dictionary + + +def restore_gff3_ids(new_to_orig_ids, file_path, output_file_name): + # Write versions + with open(f"versions.yml", "w") as f_versions: + f_versions.write('"${task.process}":\\n') + f_versions.write(f" python: {python_version()}\\n") + + with open(file_path, "r") as input_gff3_file: + input_lines = input_gff3_file.readlines() + + with open(output_file_name, "w") as output_gff_file: + for line in input_lines: + if line.startswith("##"): + output_gff_file.write(line) + continue + + new_id = line.split("\\t")[0] + orig_id = new_to_orig_ids[new_id] + output_gff_file.write("\\t".join([orig_id] + line.split("\\t")[1:])) + + +if __name__ == "__main__": + new_to_orig_ids = create_name_mapping_from_tsv(ids_tsv) + restore_gff3_ids(new_to_orig_ids, input_gff3, f"{output_prefix}.restored.ids.gff3") diff --git a/modules/pfr/custom/restoregffids/tests/main.nf.test b/modules/pfr/custom/restoregffids/tests/main.nf.test new file mode 100644 index 00000000..521b9248 --- /dev/null +++ b/modules/pfr/custom/restoregffids/tests/main.nf.test @@ -0,0 +1,63 @@ +nextflow_process { + + name "Test Process CUSTOM_RESTOREGFFIDS" + script "../main.nf" + process "CUSTOM_RESTOREGFFIDS" + + tag "modules" + tag "modules_nfcore" + tag "custom" + tag "custom/restoregffids" + + test("sarscov2-genome_gff3-success") { + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) + ] + input[1] = Channel.of('Chr1\tMT192765.1').collectFile(name: 'id_map.tsv', newLine: true) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert path(process.out.restored_ids_gff3.get(0).get(1)).getText().contains("Chr1") }, + { assert !path(process.out.restored_ids_gff3.get(0).get(1)).getText().contains("MT192765.1") }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("stub") { + + options '-stub' + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) + ] + input[1] = Channel.of('Chr1\tMT192765.1').collectFile(name: 'id_map.tsv', newLine: true) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert process.out.restored_ids_gff3 != null }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + +} diff --git a/modules/pfr/custom/restoregffids/tests/main.nf.test.snap b/modules/pfr/custom/restoregffids/tests/main.nf.test.snap new file mode 100644 index 00000000..ffe43e74 --- /dev/null +++ b/modules/pfr/custom/restoregffids/tests/main.nf.test.snap @@ -0,0 +1,41 @@ +{ + "sarscov2-genome_gff3-success": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.restored.ids.gff3:md5,2c294938b9eb4e52d19e14725c1d92a9" + ] + ], + "1": [ + "versions.yml:md5,32d31c4f1da9a3d1be013fd163e5867e" + ], + "restored_ids_gff3": [ + [ + { + "id": "test", + "single_end": false + }, + "test.restored.ids.gff3:md5,2c294938b9eb4e52d19e14725c1d92a9" + ] + ], + "versions": [ + "versions.yml:md5,32d31c4f1da9a3d1be013fd163e5867e" + ] + } + ], + "timestamp": "2023-12-07T13:49:30.047425" + }, + "versions": { + "content": [ + [ + "versions.yml:md5,32d31c4f1da9a3d1be013fd163e5867e" + ] + ], + "timestamp": "2023-12-07T13:49:30.071175" + } +} \ No newline at end of file diff --git a/modules/pfr/custom/restoregffids/tests/tags.yml b/modules/pfr/custom/restoregffids/tests/tags.yml new file mode 100644 index 00000000..1d4b9a83 --- /dev/null +++ b/modules/pfr/custom/restoregffids/tests/tags.yml @@ -0,0 +1,2 @@ +custom/restoregffids: + - "modules/pfr/custom/restoregffids/**" diff --git a/modules/pfr/custom/shortenfastaids/environment.yml b/modules/pfr/custom/shortenfastaids/environment.yml new file mode 100644 index 00000000..e80fa7cf --- /dev/null +++ b/modules/pfr/custom/shortenfastaids/environment.yml @@ -0,0 +1,11 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "custom_shortenfastaids" +channels: + - conda-forge + - bioconda + - defaults + +dependencies: + - biopython==1.75 + - python=3.8 diff --git a/modules/pfr/custom/shortenfastaids/main.nf b/modules/pfr/custom/shortenfastaids/main.nf new file mode 100644 index 00000000..92762ef6 --- /dev/null +++ b/modules/pfr/custom/shortenfastaids/main.nf @@ -0,0 +1,34 @@ +process CUSTOM_SHORTENFASTAIDS { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/biopython:1.75': + 'biocontainers/biopython:1.75' }" + + input: + tuple val(meta), path(fasta) + + output: + tuple val(meta), path("*.short.ids.fasta") , emit: short_ids_fasta , optional: true + tuple val(meta), path("*.short.ids.tsv") , emit: short_ids_tsv , optional: true + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + prefix = task.ext.prefix ?: "${meta.id}" + template 'shorten_fasta_ids.py' + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + cat <<-END_VERSIONS > versions.yml + "${task.process}": + python: \$(python --version | cut -d' ' -f2) + biopython: \$(pip list | grep "biopython" | cut -d' ' -f3) + END_VERSIONS + """ +} diff --git a/modules/pfr/custom/shortenfastaids/meta.yml b/modules/pfr/custom/shortenfastaids/meta.yml new file mode 100644 index 00000000..2425810d --- /dev/null +++ b/modules/pfr/custom/shortenfastaids/meta.yml @@ -0,0 +1,58 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "custom_shortenfastaids" +description: | + Shortens fasta IDs and produces a new fasta along with a TSV table + consisting of original (first column) and new IDs (second column). + This module is helpful when some tools like EDTA implicitly shorten + the IDs without producing the ID map, leading to downstream mismatch + in IDs across files. +keywords: + - genome + - fasta + - ID + - shorten +tools: + - "biopython": + description: | + Biopython is a set of freely available tools for biological computation written in Python by + an international team of developers. + homepage: "https://biopython.org" + documentation: "https://biopython.org/wiki/Documentation" + tool_dev_url: "https://github.com/biopython/biopython" + doi: "10.1093/bioinformatics/btp163" + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - fasta: + type: file + description: Input fasta file + pattern: "*.{fsa,fa,fasta}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - short_ids_fasta: + type: file + description: Fasta file with shortened ids if id change is required + pattern: "*.{fsa,fa,fasta}" + - short_ids_tsv: + type: file + description: | + A TSV file with original (first column) and new ids (second column) + if id change is required + pattern: "*.tsv" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/pfr/custom/shortenfastaids/templates/shorten_fasta_ids.py b/modules/pfr/custom/shortenfastaids/templates/shorten_fasta_ids.py new file mode 100755 index 00000000..54f35bf6 --- /dev/null +++ b/modules/pfr/custom/shortenfastaids/templates/shorten_fasta_ids.py @@ -0,0 +1,181 @@ +#!/usr/bin/env python3 + +import re + +from Bio import SeqIO +from importlib.metadata import version +from platform import python_version + +# The input fasta file path +fasta_file_path = "$fasta" +output_files_prefix = "$prefix" + + +def extract_fasta_ids_and_descriptions(fasta_file_path): + fasta_file_obj = SeqIO.parse(fasta_file_path, "fasta") + + ids = [] + for record in fasta_file_obj: + ids.append((record.id, record.description)) + return ids + + +def write_fasta_with_new_ids(fasta_file_path, id_mapping, file_prefix): + old_fasta_file_obj = SeqIO.parse(fasta_file_path, "fasta") + id_map = dict(id_mapping) + + replaced_records = [] + for record in old_fasta_file_obj: + old_id = record.id + + new_id = id_map[old_id] + record.id = new_id + record.description = "" + + replaced_records.append(record) + + SeqIO.write(replaced_records, f"{file_prefix}.short.ids.fasta", "fasta") + + +def do_id_need_to_change(id_and_description, silent=False): + id = id_and_description[0] + description = id_and_description[1] + if len(id) > 13: + if not silent: + print(f"{id} has length greater than 13") + return True + + if not re.match(r"^[a-zA-Z0-9_]+\$", id): + if not silent: + print(f"{id} does not match '^[a-zA-Z0-9_]+\$'") + return True + + if description != id and description != "": + if not silent: + print(f"{id} contains a comment: {description.replace(id, '')}") + return True + + if not silent: + print(f"{id} is acceptable") + return False + + +def do_ids_need_to_change(ids_and_descriptions, silent=False): + return any( + [ + do_id_need_to_change(id_and_description, silent) + for id_and_description in ids_and_descriptions + ] + ) + + +def extract_common_patterns(ids): + pattern_counts = {} + for id in ids: + patterns = re.findall(r"[A-Za-z0_]{4,}", id) + for pattern in set(patterns): + pattern_counts[pattern] = pattern_counts.get(pattern, 0) + 1 + + common_patterns = [ + pattern for pattern, count in pattern_counts.items() if count >= 2 + ] + + if len(common_patterns) < 1: + return {} + + return {pattern: pattern[:3] for pattern in common_patterns} + + +def shorten_ids(input_ids_and_descriptions, patterns_dict): + shortened_ids = [] + + for id_and_description in input_ids_and_descriptions: + id = id_and_description[0] + description = "" # Treat description as absent as it will be removed by write_fasta_with_new_ids + if not do_id_need_to_change((id, description), silent=True): + shortened_ids.append(id) + continue + + shortened_id = shorten_id_by_pattern_replacement(patterns_dict, id) + + if not do_id_need_to_change((shortened_id, description), silent=True): + shortened_ids.append(shortened_id) + continue + + shortened_id = f"Ctg{generate_hash(id)}" + + if not do_id_need_to_change((shortened_id, description), silent=True): + shortened_ids.append(shortened_id) + continue + + raise ValueError(f"Failed to shorten id: {id} ({shortened_id})") + + return shortened_ids + + +def shorten_id_by_pattern_replacement(patterns_dict, id): + if patterns_dict == {}: + return id + + shortened_id = id + matches_for_id = match_substrings(patterns_dict.keys(), shortened_id) + + for pattern in matches_for_id: + shortened_id = re.sub( + r"({})".format(re.escape(pattern)), + patterns_dict[pattern], + shortened_id, + ) + return ( + shortened_id + if shortened_id[len(shortened_id) - 1] != "_" + else shortened_id[0 : (len(shortened_id) - 1)] + ) + + +def match_substrings(substrings, target_string): + pattern = "|".join(map(re.escape, substrings)) + matches = re.findall(pattern, target_string) + return matches + + +def generate_hash(string): + import hashlib + + hash_object = hashlib.sha1(string.encode()) + full_hash = hash_object.hexdigest() + short_hash = full_hash[:10] + return short_hash + + +def fail_if_new_ids_not_valid(ids): + if len(ids) != len(set(ids)): + raise ValueError("Th new IDs are not unique") + + +if __name__ == "__main__": + input_ids_and_descriptions = extract_fasta_ids_and_descriptions(fasta_file_path) + input_ids = [x[0] for x in input_ids_and_descriptions] + + # Write versions + with open(f"versions.yml", "w") as f_versions: + f_versions.write('"${task.process}":\\n') + f_versions.write(f" python: {python_version()}\\n") + f_versions.write(f" biopython: {version('biopython')}\\n") + + if not do_ids_need_to_change(input_ids_and_descriptions): + print("IDs have acceptable length and character. No change required.") + exit(0) + + new_ids = shorten_ids( + input_ids_and_descriptions, extract_common_patterns(input_ids) + ) + fail_if_new_ids_not_valid(new_ids) + + with open(f"{output_files_prefix}.short.ids.tsv", "w") as f: + for input_id, new_id in zip(input_ids, new_ids): + f.write(f"{input_id}\\t{new_id}\\n") + + write_fasta_with_new_ids( + fasta_file_path, zip(input_ids, new_ids), output_files_prefix + ) diff --git a/modules/pfr/custom/shortenfastaids/tests/main.nf.test b/modules/pfr/custom/shortenfastaids/tests/main.nf.test new file mode 100644 index 00000000..dc46bae5 --- /dev/null +++ b/modules/pfr/custom/shortenfastaids/tests/main.nf.test @@ -0,0 +1,131 @@ +nextflow_process { + + name "Test Process CUSTOM_SHORTENFASTAIDS" + script "../main.nf" + process "CUSTOM_SHORTENFASTAIDS" + + tag "modules" + tag "modules_nfcore" + tag "custom" + tag "custom/shortenfastaids" + + test("homo_sapiens-genome_fasta-no_change") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") }, + { assert process.out.short_ids_fasta == [] }, + { assert process.out.short_ids_tsv == [] } + ) + } + + } + + test("sarscov2-genome_fasta-pattern_change") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("homo_sapiens-genome2_fasta-length_change") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], + file(params.test_data['homo_sapiens']['genome']['genome2_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("custom_fasta-comment_change") { + + when { + process { + """ + input[0] = Channel.of('>Chr1 This is a test comment', 'AGCTAGCT') + | collectFile(name: 'sample.fasta', newLine: true) + | map { file -> [ [ id:'test' ], file ] } + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test' ], + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.versions).match("versions") }, + { assert process.out.short_ids_fasta == [] }, + { assert process.out.short_ids_tsv == [] } + ) + } + + } + +} diff --git a/modules/pfr/custom/shortenfastaids/tests/main.nf.test.snap b/modules/pfr/custom/shortenfastaids/tests/main.nf.test.snap new file mode 100644 index 00000000..8fed1b9d --- /dev/null +++ b/modules/pfr/custom/shortenfastaids/tests/main.nf.test.snap @@ -0,0 +1,170 @@ +{ + "custom_fasta-comment_change": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.short.ids.fasta:md5,c861b9d46a4d9bdba66953cff572fc5d" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.short.ids.tsv:md5,8762f2bffbdff75c2812bad72ba52bba" + ] + ], + "2": [ + "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba" + ], + "short_ids_fasta": [ + [ + { + "id": "test" + }, + "test.short.ids.fasta:md5,c861b9d46a4d9bdba66953cff572fc5d" + ] + ], + "short_ids_tsv": [ + [ + { + "id": "test" + }, + "test.short.ids.tsv:md5,8762f2bffbdff75c2812bad72ba52bba" + ] + ], + "versions": [ + "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba" + ] + } + ], + "timestamp": "2023-12-07T13:33:05.523745" + }, + "versions": { + "content": [ + [ + "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba" + ] + ], + "timestamp": "2023-12-07T13:30:30.361527" + }, + "homo_sapiens-genome_fasta-no_change": { + "content": [ + { + "0": [ + + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba" + ], + "short_ids_fasta": [ + + ], + "short_ids_tsv": [ + + ], + "versions": [ + "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba" + ] + } + ], + "timestamp": "2023-12-07T13:32:54.220188" + }, + "homo_sapiens-genome2_fasta-length_change": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.short.ids.fasta:md5,1382acd98d4cd233a8062ef01b2aaa6d" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.short.ids.tsv:md5,99c0f2a529cb595b2d8530024ed2880e" + ] + ], + "2": [ + "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba" + ], + "short_ids_fasta": [ + [ + { + "id": "test" + }, + "test.short.ids.fasta:md5,1382acd98d4cd233a8062ef01b2aaa6d" + ] + ], + "short_ids_tsv": [ + [ + { + "id": "test" + }, + "test.short.ids.tsv:md5,99c0f2a529cb595b2d8530024ed2880e" + ] + ], + "versions": [ + "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba" + ] + } + ], + "timestamp": "2023-12-07T13:33:01.924483" + }, + "sarscov2-genome_fasta-pattern_change": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.short.ids.fasta:md5,14d6f587b6d28889c5c0f985e78d602f" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.short.ids.tsv:md5,d7a2af88e8549586e5616bff6a88bd71" + ] + ], + "2": [ + "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba" + ], + "short_ids_fasta": [ + [ + { + "id": "test" + }, + "test.short.ids.fasta:md5,14d6f587b6d28889c5c0f985e78d602f" + ] + ], + "short_ids_tsv": [ + [ + { + "id": "test" + }, + "test.short.ids.tsv:md5,d7a2af88e8549586e5616bff6a88bd71" + ] + ], + "versions": [ + "versions.yml:md5,e5704a53ebea373dac3a93ae800d48ba" + ] + } + ], + "timestamp": "2023-12-07T13:32:58.12885" + } +} \ No newline at end of file diff --git a/modules/pfr/custom/shortenfastaids/tests/tags.yml b/modules/pfr/custom/shortenfastaids/tests/tags.yml new file mode 100644 index 00000000..4715b64c --- /dev/null +++ b/modules/pfr/custom/shortenfastaids/tests/tags.yml @@ -0,0 +1,2 @@ +custom/shortenfastaids: + - "modules/pfr/custom/shortenfastaids/**" diff --git a/modules/pfr/ltrfinder/environment.yml b/modules/pfr/ltrfinder/environment.yml new file mode 100644 index 00000000..7f354597 --- /dev/null +++ b/modules/pfr/ltrfinder/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "ltrfinder" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::ltr_finder_parallel=1.1" diff --git a/modules/pfr/ltrfinder/main.nf b/modules/pfr/ltrfinder/main.nf new file mode 100644 index 00000000..3e59e3c7 --- /dev/null +++ b/modules/pfr/ltrfinder/main.nf @@ -0,0 +1,53 @@ +process LTRFINDER { + tag "$meta.id" + label 'process_high' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/ltr_finder_parallel:1.1--hdfd78af_0': + 'biocontainers/ltr_finder_parallel:1.1--hdfd78af_0' }" + + input: + tuple val(meta), path(fasta) + + output: + tuple val(meta), path("*.scn") , emit: scn + tuple val(meta), path("*.gff3") , emit: gff + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + LTR_FINDER_parallel \\ + -seq $fasta \\ + -threads $task.cpus \\ + $args + + mv "${fasta}.finder.combine.scn" "${prefix}.scn" + mv "${fasta}.finder.combine.gff3" "${prefix}.gff3" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + LTR_FINDER_parallel: \$(LTR_FINDER_parallel -h | grep 'Version:' | sed 's/Version: //') + ltr_finder: \$(ltr_finder -h 2>&1 | grep 'ltr_finder' | sed 's/ltr_finder //') + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch "${prefix}.scn" + touch "${prefix}.gff3" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + LTR_FINDER_parallel: \$(LTR_FINDER_parallel -h | grep 'Version:' | sed 's/Version: //') + ltr_finder: \$(ltr_finder -h 2>&1 | grep 'ltr_finder' | sed 's/ltr_finder //') + END_VERSIONS + """ +} diff --git a/modules/pfr/ltrfinder/meta.yml b/modules/pfr/ltrfinder/meta.yml new file mode 100644 index 00000000..e3c672b9 --- /dev/null +++ b/modules/pfr/ltrfinder/meta.yml @@ -0,0 +1,60 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "ltrfinder" +description: | + Finds full-length LTR retrotranspsons in genome sequences using the + parallel version of LTR_Finder +keywords: + - genomics + - annotation + - parallel + - repeat + - long terminal retrotransposon + - retrotransposon +tools: + - "LTR_FINDER_parallel": + description: A Perl wrapper for LTR_FINDER + homepage: "https://github.com/oushujun/LTR_FINDER_parallel" + documentation: "https://github.com/oushujun/LTR_FINDER_parallel" + tool_dev_url: "https://github.com/oushujun/LTR_FINDER_parallel" + doi: "10.1186/s13100-019-0193-0" + licence: ["MIT"] + - "LTR_Finder": + description: An efficient program for finding full-length LTR retrotranspsons in genome sequences + homepage: "https://github.com/xzhub/LTR_Finder" + documentation: "https://github.com/xzhub/LTR_Finder" + tool_dev_url: "https://github.com/xzhub/LTR_Finder" + doi: "10.1093/nar/gkm286" + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fasta: + type: file + description: Genome sequences in fasta format + pattern: "*.{fsa,fa,fasta}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - scn: + type: file + description: Annotation in LTRharvest or LTR_FINDER format + pattern: "*.scn" + - gff: + type: file + description: Annotation in gff3 format + pattern: "*.gff3" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/pfr/ltrfinder/tests/main.nf.test b/modules/pfr/ltrfinder/tests/main.nf.test new file mode 100644 index 00000000..447ce34d --- /dev/null +++ b/modules/pfr/ltrfinder/tests/main.nf.test @@ -0,0 +1,72 @@ +nextflow_process { + + name "Test Process LTRFINDER" + script "../main.nf" + process "LTRFINDER" + + tag "modules" + tag "modules_nfcore" + tag "ltrfinder" + tag "gunzip/main" + + test("actinidia_chinensis-genome_21_fasta_gz-success") { + + setup { + run('GUNZIP') { + script "../../gunzip/main" + + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['actinidia_chinensis']['genome']['genome_21_fasta_gz'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = GUNZIP.out.gunzip + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(path(process.out.versions[0]).text).match("versions") } + ) + } + + } + + test("stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['actinidia_chinensis']['genome']['genome_21_fasta_gz'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(path(process.out.versions[0]).text).match("stub_versions") } + ) + } + + } + +} diff --git a/modules/pfr/ltrfinder/tests/main.nf.test.snap b/modules/pfr/ltrfinder/tests/main.nf.test.snap new file mode 100644 index 00000000..54a2cee1 --- /dev/null +++ b/modules/pfr/ltrfinder/tests/main.nf.test.snap @@ -0,0 +1,120 @@ +{ + "actinidia_chinensis-genome_21_fasta_gz-success": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.scn:md5,006193c9eaf3f552ccb0369f159e7660" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.gff3:md5,96e5305163939e4381e1b94b660dc0a2" + ] + ], + "2": [ + "versions.yml:md5,7b24225b810fa88cfb2a887de11be333" + ], + "gff": [ + [ + { + "id": "test" + }, + "test.gff3:md5,96e5305163939e4381e1b94b660dc0a2" + ] + ], + "scn": [ + [ + { + "id": "test" + }, + "test.scn:md5,006193c9eaf3f552ccb0369f159e7660" + ] + ], + "versions": [ + "versions.yml:md5,7b24225b810fa88cfb2a887de11be333" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-16T09:14:38.509965" + }, + "versions": { + "content": [ + "\"LTRFINDER\":\n LTR_FINDER_parallel: v1.1\n ltr_finder: v1.07\n" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-16T09:16:55.301422" + }, + "stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.scn:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.gff3:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,7b24225b810fa88cfb2a887de11be333" + ], + "gff": [ + [ + { + "id": "test" + }, + "test.gff3:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "scn": [ + [ + { + "id": "test" + }, + "test.scn:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,7b24225b810fa88cfb2a887de11be333" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-16T09:14:43.054758" + }, + "stub_versions": { + "content": [ + "\"LTRFINDER\":\n LTR_FINDER_parallel: v1.1\n ltr_finder: v1.07\n" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-16T09:16:59.800724" + } +} \ No newline at end of file diff --git a/modules/pfr/ltrfinder/tests/tags.yml b/modules/pfr/ltrfinder/tests/tags.yml new file mode 100644 index 00000000..006ded2c --- /dev/null +++ b/modules/pfr/ltrfinder/tests/tags.yml @@ -0,0 +1,2 @@ +ltrfinder: + - "modules/nf-core/ltrfinder/**" diff --git a/modules/pfr/ltrharvest/environment.yml b/modules/pfr/ltrharvest/environment.yml new file mode 100644 index 00000000..9337fe23 --- /dev/null +++ b/modules/pfr/ltrharvest/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "ltrharvest" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::ltr_harvest_parallel=1.1" diff --git a/modules/pfr/ltrharvest/main.nf b/modules/pfr/ltrharvest/main.nf new file mode 100644 index 00000000..1e5e06d2 --- /dev/null +++ b/modules/pfr/ltrharvest/main.nf @@ -0,0 +1,56 @@ +process LTRHARVEST { + tag "$meta.id" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/ltr_harvest_parallel:1.1--hdfd78af_0': + 'biocontainers/ltr_harvest_parallel:1.1--hdfd78af_0' }" + + input: + tuple val(meta), path(fasta) + + output: + tuple val(meta), path("*.gff3") , emit: gff3 + tuple val(meta), path("*.scn") , emit: scn + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + LTR_HARVEST_parallel \\ + -seq $fasta \\ + $args \\ + -threads $task.cpus + + mv "${fasta}.harvest.combine.gff3" \\ + "${prefix}.gff3" + + mv "${fasta}.harvest.combine.scn" \\ + "${prefix}.scn" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + LTR_HARVEST_parallel: \$(LTR_HARVEST_parallel -h | sed -n '/Version/s/Version: //p') + genometools: \$(gt --version | sed '1!d ; s/gt (GenomeTools) //') + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch "${prefix}.gff3" + touch "${prefix}.scn" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + LTR_HARVEST_parallel: \$(LTR_HARVEST_parallel -h | sed -n '/Version/s/Version: //p') + genometools: \$(gt --version | sed '1!d ; s/gt (GenomeTools) //') + END_VERSIONS + """ +} diff --git a/modules/pfr/ltrharvest/meta.yml b/modules/pfr/ltrharvest/meta.yml new file mode 100644 index 00000000..efda0670 --- /dev/null +++ b/modules/pfr/ltrharvest/meta.yml @@ -0,0 +1,60 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "ltrharvest" +description: | + Predicts LTR retrotransposons using the parallel version of GenomeTools gt-ltrharvest + utility included in the EDTA toolchain +keywords: + - genomics + - genome + - annotation + - repeat + - transposons + - retrotransposons +tools: + - "edta": + description: Extensive de-novo TE Annotator (EDTA) + homepage: "https://github.com/oushujun/EDTA" + documentation: "https://github.com/oushujun/EDTA" + tool_dev_url: "https://github.com/oushujun/EDTA" + doi: "10.1186/s13059-019-1905-y" + licence: ["GPL v3"] + - "gt": + description: "The GenomeTools genome analysis system" + homepage: "https://genometools.org/index.html" + documentation: "https://genometools.org/documentation.html" + tool_dev_url: "https://github.com/genometools/genometools" + doi: "10.1109/TCBB.2013.68" + licence: ["ISC"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fasta: + type: file + description: Input genome fasta + pattern: "*.{fsa,fa,fasta}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - gff3: + type: file + description: Predicted LTR candidates in gff3 format + pattern: "*.gff3" + - scn: + type: file + description: Predicted LTR candidates in scn format + pattern: "*.scn" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/pfr/ltrharvest/tests/main.nf.test b/modules/pfr/ltrharvest/tests/main.nf.test new file mode 100644 index 00000000..9226bc0c --- /dev/null +++ b/modules/pfr/ltrharvest/tests/main.nf.test @@ -0,0 +1,60 @@ +nextflow_process { + + name "Test Process LTRHARVEST" + script "../main.nf" + process "LTRHARVEST" + + tag "modules" + tag "modules_nfcore" + tag "ltrharvest" + + test("homo_sapiens-genome_21_fasta") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], + file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.gff3).match("gff3") }, + { assert path(process.out.scn[0][1]).text.contains("46510803 46520182 9380 46510803 46510940 138 46520042 46520182 141 86.52 0 chr21") }, + { assert snapshot(path(process.out.versions[0]).text).match("script_versions") } + ) + } + + } + + test("homo_sapiens-genome_fasta-stub") { + + options '-stub' + + when { + process { + """ + input[0] = [ + [ id:'test' ], + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(path(process.out.versions[0]).text).match("stub_versions") } + ) + } + + } + +} diff --git a/modules/pfr/ltrharvest/tests/main.nf.test.snap b/modules/pfr/ltrharvest/tests/main.nf.test.snap new file mode 100644 index 00000000..ad47c4ae --- /dev/null +++ b/modules/pfr/ltrharvest/tests/main.nf.test.snap @@ -0,0 +1,88 @@ +{ + "homo_sapiens-genome_fasta-stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.gff3:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.scn:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,51e82185b713482d1d48b6f15abe7fcc" + ], + "gff3": [ + [ + { + "id": "test" + }, + "test.gff3:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "scn": [ + [ + { + "id": "test" + }, + "test.scn:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,51e82185b713482d1d48b6f15abe7fcc" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-22T14:44:30.682167" + }, + "script_versions": { + "content": [ + "\"LTRHARVEST\":\n LTR_HARVEST_parallel: v1.1\n genometools: 1.6.5\n" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-22T14:44:26.672478" + }, + "gff3": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.gff3:md5,da13c4ba22e44ef944ddec38aa72c468" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-22T19:29:33.962761" + }, + "stub_versions": { + "content": [ + "\"LTRHARVEST\":\n LTR_HARVEST_parallel: v1.1\n genometools: 1.6.5\n" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-22T14:44:30.729166" + } +} \ No newline at end of file diff --git a/modules/pfr/ltrharvest/tests/tags.yml b/modules/pfr/ltrharvest/tests/tags.yml new file mode 100644 index 00000000..92de225e --- /dev/null +++ b/modules/pfr/ltrharvest/tests/tags.yml @@ -0,0 +1,2 @@ +ltrharvest: + - "modules/nf-core/ltrharvest/**" diff --git a/modules/pfr/ltrretriever/lai/environment.yml b/modules/pfr/ltrretriever/lai/environment.yml new file mode 100644 index 00000000..e0e49682 --- /dev/null +++ b/modules/pfr/ltrretriever/lai/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "ltrretriever_lai" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::LTR_retriever=2.9.9" diff --git a/modules/pfr/ltrretriever/lai/main.nf b/modules/pfr/ltrretriever/lai/main.nf new file mode 100644 index 00000000..464b215b --- /dev/null +++ b/modules/pfr/ltrretriever/lai/main.nf @@ -0,0 +1,71 @@ +process LTRRETRIEVER_LAI { + tag "$meta.id" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/ltr_retriever:2.9.9--hdfd78af_0': + 'biocontainers/ltr_retriever:2.9.9--hdfd78af_0' }" + + input: + tuple val(meta), path(fasta) + path pass_list + path annotation_out + path monoploid_seqs + + output: + tuple val(meta), path("*.LAI.log") , emit: log + tuple val(meta), path("*.LAI.out") , emit: lai_out , optional: true + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def monoploid_param = monoploid_seqs ? "-mono $monoploid_seqs" : '' + def lai_output_name = monoploid_seqs ? "${annotation_out}.${monoploid_seqs}.out.LAI" : "${annotation_out}.LAI" + def VERSION = 'beta3.2' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. + """ + LAI \\ + -genome $fasta \\ + -intact $pass_list \\ + -all $annotation_out \\ + -t $task.cpus \\ + $monoploid_param \\ + $args \\ + > >(tee "${prefix}.LAI.log") \\ + || echo "LAI failed! See ${prefix}.LAI.log" + + mv \\ + $lai_output_name \\ + "${prefix}.LAI.out" \\ + || echo "LAI failed to estimate assembly index. See ${prefix}.LAI.log" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + lai: $VERSION + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def monoploid_param = monoploid_seqs ? "-mono $monoploid_seqs" : '' + def lai_output_name = monoploid_seqs ? "${annotation_out}.${monoploid_seqs}.out.LAI" : "${annotation_out}.LAI" + def VERSION = 'beta3.2' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. + """ + touch "${prefix}.LAI.log" + touch "$lai_output_name" + + mv \\ + $lai_output_name \\ + "${prefix}.LAI.out" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + lai: $VERSION + END_VERSIONS + """ +} diff --git a/modules/pfr/ltrretriever/lai/meta.yml b/modules/pfr/ltrretriever/lai/meta.yml new file mode 100644 index 00000000..f84cf6ca --- /dev/null +++ b/modules/pfr/ltrretriever/lai/meta.yml @@ -0,0 +1,70 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "ltrretriever_lai" +description: | + Estimates the mean LTR sequence identity in the genome. The input genome fasta should + have short alphanumeric IDs without comments +keywords: + - genomics + - annotation + - repeat + - long terminal retrotransposon + - retrotransposon + - stats + - qc +tools: + - "lai": + description: Assessing genome assembly quality using the LTR Assembly Index (LAI) + homepage: "https://github.com/oushujun/LTR_retriever" + documentation: "https://github.com/oushujun/LTR_retriever" + tool_dev_url: "https://github.com/oushujun/LTR_retriever" + doi: "10.1093/nar/gky730" + licence: ["GPL v3"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - fasta: + type: file + description: The genome file that is used to generate everything + pattern: "*.{fsa,fa,fasta}" + - pass_list: + type: file + description: A list of intact LTR-RTs generated by LTR_retriever + pattern: "*.pass.list" + - annotation_out: + type: file + description: RepeatMasker annotation of all LTR sequences in the genome + pattern: "*.out" + - monoploid_seqs: + type: file + description: | + This parameter is mainly for ployploid genomes. User provides a list of + sequence names that represent a monoploid (1x). LAI will be calculated only + on these sequences if provided. + pattern: "*.txt" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1', single_end:false ]` + - log: + type: file + description: Log from LAI + pattern: "*.LAI.log" + - lai_out: + type: file + description: | + Output file from LAI if LAI is able to estimate the index from the inputs + pattern: "*.LAI.out" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/pfr/ltrretriever/lai/tests/main.nf.test b/modules/pfr/ltrretriever/lai/tests/main.nf.test new file mode 100644 index 00000000..df7db2cf --- /dev/null +++ b/modules/pfr/ltrretriever/lai/tests/main.nf.test @@ -0,0 +1,166 @@ +nextflow_process { + + name "Test Process LTRRETRIEVER_LAI" + script "../main.nf" + process "LTRRETRIEVER_LAI" + config "./nextflow.config" + + tag "modules" + tag "modules_nfcore" + tag "gunzip" + tag "ltrretriever" + tag "ltrretriever/ltrretriever" + tag "ltrretriever/lai" + tag "ltrharvest" + tag "ltrfinder" + tag "cat/cat" + + test("actinidia_chinensis-genome_21_fasta_gz-success") { + + setup { + + run("GUNZIP") { + script "../../../gunzip" + + process { + """ + input[0] = [ + [ id:'test' ], + file(params.test_data['actinidia_chinensis']['genome']['genome_21_fasta_gz'], checkIfExists: true) + ] + """ + } + } + + run("LTRHARVEST") { + script "../../../ltrharvest" + + process { + """ + input[0] = GUNZIP.out.gunzip + """ + } + } + + run("LTRFINDER") { + script "../../../ltrfinder" + + process { + """ + input[0] = GUNZIP.out.gunzip + """ + } + } + + run("CAT_CAT") { + script "../../../cat/cat" + + process { + """ + input[0] = LTRHARVEST.out.scn.mix(LTRFINDER.out.scn).groupTuple() + """ + } + } + + run("LTRRETRIEVER_LTRRETRIEVER") { + script "../../ltrretriever" + + process { + """ + input[0] = GUNZIP.out.gunzip + input[1] = CAT_CAT.out.file_out.map { meta, tabout -> tabout } + input[2] = [] + input[3] = [] + input[4] = [] + """ + } + } + } + + when { + process { + """ + input[0] = GUNZIP.out.gunzip + input[1] = LTRRETRIEVER_LTRRETRIEVER.out.pass_list.map { meta, pass_list -> pass_list } + input[2] = LTRRETRIEVER_LTRRETRIEVER.out.annotation_out.map { meta, annotation_out -> annotation_out } + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert path(process.out.log[0][1]).text.contains("Dependency checking: Passed!") }, + { assert path(process.out.log[0][1]).text.contains("Calculate LAI:") }, + { assert path(process.out.log[0][1]).text.contains("Done!") }, + { assert path(process.out.log[0][1]).text.contains("Result file:") }, + { assert Math.abs(Float.parseFloat(path(process.out.lai_out[0][1]).text.split("\n")[1].split("\t")[6]) - 31.29) <= 1.0 } + ) + } + + } + + test("stub") { + + options '-stub' + + when { + process { + """ + def pass_list = new File('test.pass.list') + def out_file = new File('test.out') + def monoploid_seqs = new File('some_seqs.list.txt') + + input[0] = [ + [ id:'test' ], + file(params.test_data['actinidia_chinensis']['genome']['genome_21_fasta_gz'], checkIfExists: true) + ] + input[1] = pass_list.toPath() + input[2] = out_file.toPath() + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("stub_with_monoploid_seqs") { + + options '-stub' + + when { + process { + """ + def pass_list = new File('test.pass.list') + def out_file = new File('test.out') + def monoploid_seqs = new File('some_seqs.list.txt') + + input[0] = [ + [ id:'test' ], + file(params.test_data['actinidia_chinensis']['genome']['genome_21_fasta_gz'], checkIfExists: true) + ] + input[1] = pass_list.toPath() + input[2] = out_file.toPath() + input[3] = monoploid_seqs.toPath() + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/pfr/ltrretriever/lai/tests/main.nf.test.snap b/modules/pfr/ltrretriever/lai/tests/main.nf.test.snap new file mode 100644 index 00000000..e1c8086b --- /dev/null +++ b/modules/pfr/ltrretriever/lai/tests/main.nf.test.snap @@ -0,0 +1,100 @@ +{ + "stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.LAI.log:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.LAI.out:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,e04e27f9408e771795cd44d96518b7cd" + ], + "lai_out": [ + [ + { + "id": "test" + }, + "test.LAI.out:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "log": [ + [ + { + "id": "test" + }, + "test.LAI.log:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,e04e27f9408e771795cd44d96518b7cd" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-22T20:09:00.558021" + }, + "stub_with_monoploid_seqs": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.LAI.log:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.LAI.out:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,e04e27f9408e771795cd44d96518b7cd" + ], + "lai_out": [ + [ + { + "id": "test" + }, + "test.LAI.out:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "log": [ + [ + { + "id": "test" + }, + "test.LAI.log:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,e04e27f9408e771795cd44d96518b7cd" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-22T20:10:08.213842" + } +} \ No newline at end of file diff --git a/modules/pfr/ltrretriever/lai/tests/nextflow.config b/modules/pfr/ltrretriever/lai/tests/nextflow.config new file mode 100644 index 00000000..75edf1a9 --- /dev/null +++ b/modules/pfr/ltrretriever/lai/tests/nextflow.config @@ -0,0 +1,15 @@ +process { + + withName: LTRHARVEST { + ext.prefix = { "${meta.id}_ltrharvest" } + } + + withName: LTRFINDER { + ext.args = '-harvest_out -size 1000000 -time 300' + // recommended parameters: https://github.com/oushujun/LTR_retriever#usage + } + + withName: CAT_CAT { + ext.prefix = { "${meta.id}_ltrharvest_ltrfinder.tabout" } + } +} diff --git a/modules/pfr/ltrretriever/lai/tests/tags.yml b/modules/pfr/ltrretriever/lai/tests/tags.yml new file mode 100644 index 00000000..470f4687 --- /dev/null +++ b/modules/pfr/ltrretriever/lai/tests/tags.yml @@ -0,0 +1,2 @@ +ltrretriever/lai: + - "modules/nf-core/ltrretriever/lai/**" diff --git a/modules/pfr/ltrretriever/ltrretriever/environment.yml b/modules/pfr/ltrretriever/ltrretriever/environment.yml new file mode 100644 index 00000000..f49f07e7 --- /dev/null +++ b/modules/pfr/ltrretriever/ltrretriever/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "ltrretriever_ltrretriever" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::LTR_retriever=2.9.9" diff --git a/modules/pfr/ltrretriever/ltrretriever/main.nf b/modules/pfr/ltrretriever/ltrretriever/main.nf new file mode 100644 index 00000000..f4577920 --- /dev/null +++ b/modules/pfr/ltrretriever/ltrretriever/main.nf @@ -0,0 +1,77 @@ +process LTRRETRIEVER_LTRRETRIEVER { + tag "$meta.id" + label 'process_high' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/ltr_retriever:2.9.9--hdfd78af_0': + 'biocontainers/ltr_retriever:2.9.9--hdfd78af_0' }" + + input: + tuple val(meta), path(genome) + path(harvest) + path(finder) + path(mgescan) + path(non_tgca) + + output: + tuple val(meta), path("*.log") , emit: log + tuple val(meta), path("${prefix}.pass.list"), emit: pass_list + tuple val(meta), path("*.pass.list.gff3") , emit: pass_list_gff + tuple val(meta), path("*.LTRlib.fa") , emit: ltrlib + tuple val(meta), path("${prefix}.out") , emit: annotation_out , optional: true + tuple val(meta), path("*.out.gff3") , emit: annotation_gff , optional: true + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + prefix = task.ext.prefix ?: "${meta.id}" + def inharvest = harvest ? "-inharvest $harvest" : '' + def infinder = finder ? "-infinder $finder" : '' + def inmgescan = mgescan ? "-inmgescan $mgescan" : '' + def non_tgca_file = non_tgca ? "-nonTGCA $non_tgca" : '' + """ + LTR_retriever \\ + -genome $genome \\ + $inharvest \\ + $infinder \\ + $inmgescan \\ + $non_tgca_file \\ + -threads $task.cpus \\ + $args \\ + &> >(tee "${prefix}.log" 2>&1) + + mv "${genome}.pass.list" "${prefix}.pass.list" + mv "${genome}.pass.list.gff3" "${prefix}.pass.list.gff3" + mv "${genome}.LTRlib.fa" "${prefix}.LTRlib.fa" + mv "${genome}.out" "${prefix}.out" || echo ".out was not produced" + mv "${genome}.out.gff3" "${prefix}.out.gff3" || echo ".out.gff3 was not produced" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + LTR_retriever: \$(LTR_retriever -h 2>&1 | grep '### LTR_retriever' | sed 's/### LTR_retriever //; s/ ###//') + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + prefix = task.ext.prefix ?: "${meta.id}" + def touch_out = args.contains('-noanno') ? '' : "touch ${prefix}.out" + def touch_out_gff = args.contains('-noanno') ? '' : "touch ${prefix}.out.gff3" + """ + touch "${prefix}.log" + touch "${prefix}.pass.list" + touch "${prefix}.pass.list.gff3" + touch "${prefix}.LTRlib.fa" + $touch_out + $touch_out_gff + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + LTR_retriever: \$(LTR_retriever -h 2>&1 | grep '### LTR_retriever' | sed 's/### LTR_retriever //; s/ ###//') + END_VERSIONS + """ +} diff --git a/modules/pfr/ltrretriever/ltrretriever/meta.yml b/modules/pfr/ltrretriever/ltrretriever/meta.yml new file mode 100644 index 00000000..a310b04a --- /dev/null +++ b/modules/pfr/ltrretriever/ltrretriever/meta.yml @@ -0,0 +1,83 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "ltrretriever_ltrretriever" +description: Identifies LTR retrotransposons using LTR_retriever +keywords: + - genomics + - annotation + - repeat + - long terminal repeat + - retrotransposon +tools: + - "LTR_retriever": + description: Sensitive and accurate identification of LTR retrotransposons + homepage: "https://github.com/oushujun/LTR_retriever" + documentation: "https://github.com/oushujun/LTR_retriever" + tool_dev_url: "https://github.com/oushujun/LTR_retriever" + doi: "10.1104/pp.17.01310" + licence: ["GPL v3"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - genome: + type: file + description: Genomic sequences in fasta format + pattern: "*.{fsa,fa,fasta}" + - harvest: + type: file + description: LTR-RT candidates from GenomeTools ltrharvest in the old tabular format + pattern: "*.tabout" + - finder: + type: file + description: LTR-RT candidates from LTR_FINDER + pattern: "*.scn" + - mgescan: + type: file + description: LTR-RT candidates from MGEScan_LTR + pattern: "*.out" + - non_tgca: + type: file + description: Non-canonical LTR-RT candidates from GenomeTools ltrharvest in the old tabular format + pattern: "*.tabout" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - log: + type: file + description: Output log from LTR_retriever + pattern: "*.log" + - pass_list: + type: file + description: Intact LTR-RTs with coordinate and structural information in summary table format + pattern: "*.pass.list" + - pass_list_gff: + type: file + description: Intact LTR-RTs with coordinate and structural information in gff3 format + pattern: "*.pass.list.gff3" + - ltrlib: + type: file + description: All non-redundant LTR-RTs + pattern: "*.LTRlib.fa" + - annotation_out: + type: file + description: Whole-genome LTR-RT annotation by the non-redundant library + pattern: "*.out" + - annotation_gff: + type: file + description: Whole-genome LTR-RT annotation by the non-redundant library in gff3 format + pattern: "*.out.gff3" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/pfr/ltrretriever/ltrretriever/tests/main.nf.test b/modules/pfr/ltrretriever/ltrretriever/tests/main.nf.test new file mode 100644 index 00000000..f6ab43db --- /dev/null +++ b/modules/pfr/ltrretriever/ltrretriever/tests/main.nf.test @@ -0,0 +1,133 @@ +nextflow_process { + + name "Test Process LTRRETRIEVER_LTRRETRIEVER" + script "../main.nf" + process "LTRRETRIEVER_LTRRETRIEVER" + config "./nextflow.config" + + tag "modules" + tag "modules_nfcore" + tag "ltrretriever" + tag "ltrretriever/ltrretriever" + tag "gunzip/main" + tag "gt/ltrharvest" + tag "gt/suffixerator" + tag "ltrfinder" + tag "cat/cat" + + test("actinidia_chinensis-genome_21_fasta_gz-success") { + + setup { + + run('GUNZIP') { + script "../../../gunzip/main" + + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['actinidia_chinensis']['genome']['genome_21_fasta_gz'], checkIfExists: true) + ] + """ + } + } + + run("GT_SUFFIXERATOR") { + script "../../../gt/suffixerator" + + process { + """ + input[0] = GUNZIP.out.gunzip + input[1] = 'dna' + """ + } + } + + run("GT_LTRHARVEST") { + script "../../../gt/ltrharvest" + + process { + """ + input[0] = GT_SUFFIXERATOR.out.index + """ + } + } + + run("LTRFINDER") { + script "../../../ltrfinder" + + process { + """ + input[0] = GUNZIP.out.gunzip + """ + } + } + + run("CAT_CAT") { + script "../../../cat/cat" + + process { + """ + input[0] = GT_LTRHARVEST.out.tabout.mix(LTRFINDER.out.scn).groupTuple() + """ + } + } + } + + when { + process { + """ + input[0] = GUNZIP.out.gunzip + input[1] = CAT_CAT.out.file_out.map { meta, tabout -> tabout } + input[2] = [] + input[3] = [] + input[4] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert path(process.out.log[0][1]).text.contains("####### Result files #########") }, + { assert snapshot(process.out.pass_list).match("pass_list") }, + { assert path(process.out.pass_list_gff[0][1]).text.contains("chr1\tLTR_retriever\ttarget_site_duplication") }, + { assert path(process.out.ltrlib[0][1]).text.contains("LTR#LTR/Copia") }, + { assert snapshot(process.out.annotation_out).match("annotation_out") }, + { assert path(process.out.annotation_gff[0][1]).text.contains("Classification=LTR/Copia") }, + { assert snapshot(path(process.out.versions[0]).text).match("versions") } + ) + } + + } + + test("stub") { + + options '-stub' + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['actinidia_chinensis']['genome']['genome_21_fasta_gz'], checkIfExists: true) + ] + input[1] = [] + input[2] = [] + input[3] = [] + input[4] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(path(process.out.versions[0]).text).match("versions_stub") } + ) + } + + } + +} diff --git a/modules/pfr/ltrretriever/ltrretriever/tests/main.nf.test.snap b/modules/pfr/ltrretriever/ltrretriever/tests/main.nf.test.snap new file mode 100644 index 00000000..bcf98638 --- /dev/null +++ b/modules/pfr/ltrretriever/ltrretriever/tests/main.nf.test.snap @@ -0,0 +1,169 @@ +{ + "versions_stub": { + "content": [ + "\"LTRRETRIEVER_LTRRETRIEVER\":\n LTR_retriever: v2.9.9\n" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-19T11:04:16.007262" + }, + "pass_list": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.pass.list:md5,0c96ee3b48691e65da2235786a926160" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-19T11:17:50.087449" + }, + "versions": { + "content": [ + "\"LTRRETRIEVER_LTRRETRIEVER\":\n LTR_retriever: v2.9.9\n" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-19T11:17:50.208819" + }, + "stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.log:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.pass.list:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + [ + { + "id": "test" + }, + "test.pass.list.gff3:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "3": [ + [ + { + "id": "test" + }, + "test.LTRlib.fa:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "4": [ + [ + { + "id": "test" + }, + "test.out:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "5": [ + [ + { + "id": "test" + }, + "test.out.gff3:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "6": [ + "versions.yml:md5,3ab159acaee06b342b56e2d35e5e669b" + ], + "annotation_gff": [ + [ + { + "id": "test" + }, + "test.out.gff3:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "annotation_out": [ + [ + { + "id": "test" + }, + "test.out:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "log": [ + [ + { + "id": "test" + }, + "test.log:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "ltrlib": [ + [ + { + "id": "test" + }, + "test.LTRlib.fa:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "pass_list": [ + [ + { + "id": "test" + }, + "test.pass.list:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "pass_list_gff": [ + [ + { + "id": "test" + }, + "test.pass.list.gff3:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,3ab159acaee06b342b56e2d35e5e669b" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-19T11:04:15.954424" + }, + "annotation_out": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.out:md5,4ecf9226cbd7a3aaf7cf5cfa575fcc6a" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-19T11:17:50.150622" + } +} \ No newline at end of file diff --git a/modules/pfr/ltrretriever/ltrretriever/tests/nextflow.config b/modules/pfr/ltrretriever/ltrretriever/tests/nextflow.config new file mode 100644 index 00000000..11499594 --- /dev/null +++ b/modules/pfr/ltrretriever/ltrretriever/tests/nextflow.config @@ -0,0 +1,21 @@ +process { + + withName: GT_SUFFIXERATOR { + ext.args = '-suf -lcp' + // GT_LTRHARVEST requires -suf, -lcp + } + + withName: LTRFINDER { + ext.args = '-harvest_out' + // LTRRETRIEVER requires -harvest_out + } + + withName: GT_LTRHARVEST { + ext.args = '-minlenltr 100 -maxlenltr 7000 -mintsd 4 -maxtsd 6 -motif TGCA -motifmis 1 -similar 85 -vic 10 -seed 20 -seqids yes' + // recommended parameters: https://github.com/oushujun/LTR_retriever#usage + } + + withName: CAT_CAT { + ext.prefix = { "${meta.id}_ltrharvest_ltrfinder.tabout" } + } +} diff --git a/modules/pfr/ltrretriever/ltrretriever/tests/tags.yml b/modules/pfr/ltrretriever/ltrretriever/tests/tags.yml new file mode 100644 index 00000000..67241ccb --- /dev/null +++ b/modules/pfr/ltrretriever/ltrretriever/tests/tags.yml @@ -0,0 +1,2 @@ +ltrretriever/ltrretriever: + - "modules/nf-core/ltrretriever/ltrretriever/**" diff --git a/subworkflows/pfr/fasta_ltrretriever_lai/main.nf b/subworkflows/pfr/fasta_ltrretriever_lai/main.nf new file mode 100644 index 00000000..566e6821 --- /dev/null +++ b/subworkflows/pfr/fasta_ltrretriever_lai/main.nf @@ -0,0 +1,114 @@ +include { CUSTOM_SHORTENFASTAIDS } from '../../../modules/pfr/custom/shortenfastaids/main' +include { LTRHARVEST } from '../../../modules/pfr/ltrharvest/main' +include { LTRFINDER } from '../../../modules/pfr/ltrfinder/main' +include { LTRRETRIEVER_LTRRETRIEVER } from '../../../modules/pfr/ltrretriever/ltrretriever/main' +include { CAT_CAT } from '../../../modules/pfr/cat/cat/main' +include { LTRRETRIEVER_LAI } from '../../../modules/pfr/ltrretriever/lai/main' +include { CUSTOM_RESTOREGFFIDS } from '../../../modules/pfr/custom/restoregffids/main' + +workflow FASTA_LTRRETRIEVER_LAI { + + take: + ch_fasta // channel: [ val(meta), fasta ] + ch_monoploid_seqs // channel: [ val(meta), txt ]; Optional: Set to [] if not needed + skip_lai // val; true|false + + main: + + ch_versions = Channel.empty() + + // MOUDLE: CUSTOM_SHORTENFASTAIDS + CUSTOM_SHORTENFASTAIDS ( ch_fasta ) + + ch_short_ids_fasta = ch_fasta + | join(CUSTOM_SHORTENFASTAIDS.out.short_ids_fasta, by:0, remainder:true) + | map { meta, fasta, short_ids_fasta -> + [ meta, short_ids_fasta ?: fasta ] + } + + ch_short_ids_tsv = CUSTOM_SHORTENFASTAIDS.out.short_ids_tsv + ch_versions = ch_versions.mix(CUSTOM_SHORTENFASTAIDS.out.versions.first()) + + // MODULE: LTRHARVEST + LTRHARVEST ( ch_short_ids_fasta ) + + ch_ltrharvest_scn = LTRHARVEST.out.scn + ch_versions = ch_versions.mix(LTRHARVEST.out.versions.first()) + + // MODULE: LTRFINDER + LTRFINDER ( ch_short_ids_fasta ) + + ch_ltrfinder_scn = LTRFINDER.out.scn + ch_versions = ch_versions.mix(LTRFINDER.out.versions.first()) + + // MODULE: CAT_CAT + ch_cat_cat_inputs = ch_ltrharvest_scn + | join(ch_ltrfinder_scn) + | map { meta, harvested, found -> [ meta, [ harvested, found ] ] } + + CAT_CAT ( ch_cat_cat_inputs ) + + ch_ltr_candidates = CAT_CAT.out.file_out + ch_versions = ch_versions.mix(CAT_CAT.out.versions.first()) + + // MODULE: LTRRETRIEVER_LTRRETRIEVER + ch_ltrretriever_inputs = ch_short_ids_fasta.join(ch_ltr_candidates) + LTRRETRIEVER_LTRRETRIEVER ( + ch_ltrretriever_inputs.map { meta, fasta, ltr -> [ meta, fasta ] }, + ch_ltrretriever_inputs.map { meta, fasta, ltr -> ltr }, + [], + [], + [] + ) + + ch_pass_list = LTRRETRIEVER_LTRRETRIEVER.out.pass_list + ch_ltrlib = LTRRETRIEVER_LTRRETRIEVER.out.ltrlib + ch_annotation_out = LTRRETRIEVER_LTRRETRIEVER.out.annotation_out + ch_annotation_gff = LTRRETRIEVER_LTRRETRIEVER.out.annotation_gff + ch_versions = ch_versions.mix(LTRRETRIEVER_LTRRETRIEVER.out.versions.first()) + + // MODULE: LAI + ch_lai_inputs = skip_lai + ? Channel.empty() + : ch_short_ids_fasta + | join(ch_pass_list) + | join(ch_annotation_out) + | join( + ch_monoploid_seqs ?: Channel.empty(), + by:0, + remainder: true + ) + | map { meta, fasta, pass, out, mono -> + [ meta, fasta, pass, out, mono ?: [] ] + } + LTRRETRIEVER_LAI( + ch_lai_inputs.map { meta, fasta, pass, out, mono -> [ meta, fasta ] }, + ch_lai_inputs.map { meta, fasta, pass, out, mono -> pass }, + ch_lai_inputs.map { meta, fasta, pass, out, mono -> out }, + ch_lai_inputs.map { meta, fasta, pass, out, mono -> mono } + ) + + ch_lai_log = LTRRETRIEVER_LAI.out.log + ch_lai_out = LTRRETRIEVER_LAI.out.lai_out + ch_versions = ch_versions.mix(LTRRETRIEVER_LAI.out.versions.first()) + + // MODULE: CUSTOM_RESTOREGFFIDS + ch_restorable_gff_tsv = ch_annotation_gff.join(ch_short_ids_tsv) + + CUSTOM_RESTOREGFFIDS ( + ch_restorable_gff_tsv.map { meta, gff, tsv -> [ meta, gff ] }, + ch_restorable_gff_tsv.map { meta, gff, tsv -> tsv } + ) + + ch_restored_gff = ch_annotation_gff + | join(CUSTOM_RESTOREGFFIDS.out.restored_ids_gff3, by:0, remainder:true) + | map { meta, gff, restored_gff -> [ meta, restored_gff ?: gff ] } + ch_versions = ch_versions.mix(CUSTOM_RESTOREGFFIDS.out.versions.first()) + + emit: + ltrlib = ch_ltrlib // channel: [ val(meta), fasta ] + annotation_gff = ch_restored_gff // channel: [ val(meta), gff ] + lai_log = ch_lai_log // channel: [ val(meta), log ] + lai_out = ch_lai_out // channel: [ val(meta), out ] + versions = ch_versions // channel: [ versions.yml ] +} diff --git a/subworkflows/pfr/fasta_ltrretriever_lai/meta.yml b/subworkflows/pfr/fasta_ltrretriever_lai/meta.yml new file mode 100644 index 00000000..5ba17303 --- /dev/null +++ b/subworkflows/pfr/fasta_ltrretriever_lai/meta.yml @@ -0,0 +1,78 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "fasta_ltrretriever_lai" +description: | + Performs long terminal retrotransposons (LTR) annotation with gt/ltrharvest, ltrfinder + and ltrretriever along with LTR assembly index (LAI). +keywords: + - genomics + - genome + - annotation + - repeat + - transposons + - retrotransposons + - stats + - qc +components: + - custom/shortenfastaids + - ltrharvest + - ltrfinder + - ltrretriever/ltrretriever + - cat/cat + - ltrretriever/lai + - custom/restoregffids +input: + - ch_fasta: + type: file + description: | + Channel for the assembly fasta file + Structure: [ val(meta), path(fasta) ] + pattern: "*.{fsa/fa/fasta}" + - ch_monoploid_seqs: + type: file + description: | + Channel for providing a list of monoploid sequences + for correct estimation of LAI for polyploid genomes. + This parameter is useful when all the haplotypes are + stored in a single fasta file. + Structure: [ val(meta), path(txt) ] + pattern: "*.txt" + - skip_lai: + type: boolean + description: | + Skip LAI estimation + Structure: [ val(boolean) ] +output: + - ltrlib: + type: file + description: | + LTR library constructed by ltrretriever + Structure: [ val(meta), path(fasta) ] + pattern: "*.LTRlib.fa" + - annotation_gff: + type: file + description: | + Annotation based on the LTR library + Structure: [ val(meta), path(gff3) ] + pattern: "*.gff3" + - lai_log: + type: file + description: | + Log from LAI + Structure: [ val(meta), path(log) ] + pattern: "*.LAI.log" + - lai_out: + type: file + description: | + LAI output + Structure: [ val(meta), path(out) ] + pattern: "*.LAI.out" + - versions: + type: file + description: | + File containing software versions + Structure: [ path(versions.yml) ] + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test b/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test new file mode 100644 index 00000000..773c514a --- /dev/null +++ b/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test @@ -0,0 +1,59 @@ +nextflow_workflow { + + name "Test Workflow FASTA_LTRRETRIEVER_LAI" + script "../main.nf" + workflow "FASTA_LTRRETRIEVER_LAI" + config "./nextflow.config" + + tag "subworkflows" + tag "subworkflows_nfcore" + tag "subworkflows/fasta_ltrretriever_lai" + tag "fasta_ltrretriever_lai" + tag "modules/nf-core/gunzip" + tag "custom/shortenfastaids" + tag "ltrharvest" + tag "ltrfinder" + tag "ltrretriever/ltrretriever" + tag "cat/cat" + tag "ltrretriever/lai" + tag "custom/restoregffids" + + test("actinidia_chinensis-genome_21_fasta_gz-success") { + + setup { + run("GUNZIP") { + script "../../../../modules/nf-core/gunzip" + + process { + """ + input[0] = [ + [ id:'test' ], + file(params.test_data['actinidia_chinensis']['genome']['genome_21_fasta_gz'], checkIfExists: true) + ] + """ + } + } + } + + when { + workflow { + """ + input[0] = GUNZIP.out.gunzip + input[1] = [] + input[2] = false + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert file(workflow.out.annotation_gff[0][1]).text.contains('Copia_LTR_retrotransposon') }, + { assert file(workflow.out.lai_log[0][1]).text.contains('Calculate LAI:') }, + { assert file(workflow.out.lai_log[0][1]).text.contains('Done!') }, + { assert Math.abs(Float.parseFloat(path(workflow.out.lai_out[0][1]).text.split("\n")[1].split("\t")[6]) - 31.29) <= 1.0 }, + { assert file(workflow.out.ltrlib[0][1]).text.contains('#LTR/Copia') } + ) + } + } +} diff --git a/subworkflows/pfr/fasta_ltrretriever_lai/tests/nextflow.config b/subworkflows/pfr/fasta_ltrretriever_lai/tests/nextflow.config new file mode 100644 index 00000000..617b1160 --- /dev/null +++ b/subworkflows/pfr/fasta_ltrretriever_lai/tests/nextflow.config @@ -0,0 +1,14 @@ +process { + + withName: LTRHARVEST { + ext.prefix = { "${meta.id}_ltrharvest" } + } + + withName: LTRFINDER { + ext.args = '-harvest_out -size 1000000 -time 300' + } + + withName: CAT_CAT { + ext.prefix = { "${meta.id}_ltrharvest_ltrfinder.tabout" } + } +} diff --git a/subworkflows/pfr/fasta_ltrretriever_lai/tests/tags.yml b/subworkflows/pfr/fasta_ltrretriever_lai/tests/tags.yml new file mode 100644 index 00000000..9c15c67e --- /dev/null +++ b/subworkflows/pfr/fasta_ltrretriever_lai/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/fasta_ltrretriever_lai: + - subworkflows/pfr/fasta_ltrretriever_lai/** diff --git a/tests/stub/assemblysheet.csv b/tests/stub/assemblysheet.csv index 86b2de82..dd67ad3b 100644 --- a/tests/stub/assemblysheet.csv +++ b/tests/stub/assemblysheet.csv @@ -1,2 +1,2 @@ tag,fasta,gff3,monoploid_ids,hic_reads,synteny_labels -FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz,https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/docs/test_files/FI1.monoploid.seqs.txt,"https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/docs/test_files/hic/stub_hic.R{1,2}.fq.gz",https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/FI1.seq.labels.tsv +FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz,https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/tests/stub/FI1.monoploid.seqs.txt,"https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/docs/test_files/hic/stub_hic.R{1,2}.fq.gz",https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/FI1.seq.labels.tsv diff --git a/tests/stub/stub.config b/tests/stub/stub.config index 6daf7008..d97be7eb 100644 --- a/tests/stub/stub.config +++ b/tests/stub/stub.config @@ -15,6 +15,8 @@ params { tidk_skip = false + lai_skip = false + // Limit resources so that this can run on GitHub Actions max_cpus = 2 max_memory = '6.GB' diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index cef4bb86..a6e44f48 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -33,6 +33,7 @@ include { NCBI_FCS_ADAPTOR } from '../modules/local/ncbi_fcs_ad include { NCBI_FCS_GX } from '../subworkflows/local/ncbi_fcs_gx' include { ASSEMBLATHON_STATS } from '../modules/local/assemblathon_stats' include { FASTA_BUSCO_PLOT } from '../subworkflows/local/fasta_busco_plot' +include { FASTA_LTRRETRIEVER_LAI } from '../subworkflows/pfr/fasta_ltrretriever_lai/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -63,11 +64,12 @@ def assemblyqc_report = [] workflow ASSEMBLYQC { + // Input channels ch_versions = Channel.empty() ch_input = Channel.fromSamplesheet('input') ch_target_assemby_branch = ch_input - | map { tag, fasta, gff, ids, reads, labels -> + | map { tag, fasta, gff, mono_ids, reads, labels -> [ [ id: tag ], file(fasta, checkIfExists: true) ] } | branch { meta, fasta -> @@ -76,7 +78,7 @@ workflow ASSEMBLYQC { } ch_assemby_gff3_branch = ch_input - | map { tag, fasta, gff, ids, reads, labels -> + | map { tag, fasta, gff, mono_ids, reads, labels -> gff ? [ [ id: tag ], file(gff, checkIfExists: true) ] : null @@ -86,6 +88,13 @@ workflow ASSEMBLYQC { rest: !"$gff".endsWith(".gz") } + ch_mono_ids = ch_input + | map { tag, fasta, gff, mono_ids, reads, labels -> + mono_ids + ? [ [ id: tag ], file(mono_ids, checkIfExists: true) ] + : null + } + // MODULE: GUNZIP as GUNZIP_FASTA GUNZIP_FASTA ( ch_target_assemby_branch.gz ) @@ -303,6 +312,29 @@ workflow ASSEMBLYQC { ch_versions = ch_versions.mix(FASTA_EXPLORE_SEARCH_PLOT_TIDK.out.versions) + // SUBWORKFLOW: FASTA_LTRRETRIEVER_LAI + ch_lai_inputs = params.lai_skip + ? Channel.empty() + : ch_clean_assembly + | join( + ch_mono_ids + | map { meta, mono -> [ meta.id, mono ] }, + remainder: true + ) + | map { id, fasta, mono -> [ id, fasta, mono ?: [] ] } + + FASTA_LTRRETRIEVER_LAI( + ch_lai_inputs.map { id, fasta, mono -> [ [ id:id ], fasta ] }, + ch_lai_inputs.map { id, fasta, mono -> [ [ id:id ], mono ] }, + false // Not skipping LAI using this flag + ) + + ch_lai_outputs = FASTA_LTRRETRIEVER_LAI.out.lai_log + | join(FASTA_LTRRETRIEVER_LAI.out.lai_out, remainder: true) + | map { meta, log, out -> out ? [ log, out ] : [log] } + + ch_versions = ch_versions.mix(FASTA_LTRRETRIEVER_LAI.out.versions) + // MODULE: CUSTOM_DUMPSOFTWAREVERSIONS CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') From 43255b6db136601a8efd0b05dcdb6b8e6563b989 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Fri, 23 Feb 2024 10:49:01 +1300 Subject: [PATCH 25/65] Added fasta_kraken2 --- conf/modules.config | 10 ++- lib/WorkflowMain.groovy | 5 ++ modules.json | 5 ++ modules/local/kraken2.nf | 47 ++++++++++++++ modules/local/kraken2_krona_plot.nf | 27 ++++++++ modules/nf-core/untar/environment.yml | 11 ++++ modules/nf-core/untar/main.nf | 63 +++++++++++++++++++ modules/nf-core/untar/meta.yml | 46 ++++++++++++++ modules/nf-core/untar/tests/main.nf.test | 55 ++++++++++++++++ modules/nf-core/untar/tests/main.nf.test.snap | 34 ++++++++++ modules/nf-core/untar/tests/tags.yml | 2 + subworkflows/local/fasta_kraken2.nf | 44 +++++++++++++ tests/stub/kraken2/k2_minusb_20231009.tar.gz | 0 tests/stub/stub.config | 3 + workflows/assemblyqc.nf | 17 +++++ 15 files changed, 368 insertions(+), 1 deletion(-) create mode 100644 modules/local/kraken2.nf create mode 100644 modules/local/kraken2_krona_plot.nf create mode 100644 modules/nf-core/untar/environment.yml create mode 100644 modules/nf-core/untar/main.nf create mode 100644 modules/nf-core/untar/meta.yml create mode 100644 modules/nf-core/untar/tests/main.nf.test create mode 100644 modules/nf-core/untar/tests/main.nf.test.snap create mode 100644 modules/nf-core/untar/tests/tags.yml create mode 100644 subworkflows/local/fasta_kraken2.nf create mode 100644 tests/stub/kraken2/k2_minusb_20231009.tar.gz diff --git a/conf/modules.config b/conf/modules.config index a22b25d8..60beb771 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -71,7 +71,15 @@ process { ] } - withName: RUN_KRAKEN2 { + withName: KRAKEN2 { + publishDir = [ + path: { "${params.outdir}/kraken2" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: KRAKEN2_KRONA_PLOT { publishDir = [ path: { "${params.outdir}/kraken2" }, mode: params.publish_dir_mode, diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy index c6c8b504..2b6cfbbe 100755 --- a/lib/WorkflowMain.groovy +++ b/lib/WorkflowMain.groovy @@ -57,6 +57,11 @@ class WorkflowMain { if (!params.busco_skip && !params.busco_lineage_datasets) { Nextflow.error('busco_lineage_datasets must be provided when executing BUSCO') } + + // Check for kraken2_db_path + if (!params.kraken2_skip && !params.kraken2_db_path) { + Nextflow.error('kraken2_db_path must be provided when executing Kraken2') + } } // // Get attribute from genome config file e.g. fasta diff --git a/modules.json b/modules.json index b9e6d4f1..d9161f6a 100644 --- a/modules.json +++ b/modules.json @@ -119,6 +119,11 @@ "branch": "master", "git_sha": "669a329f4aa37f5b7f03776c2ed1cd0ef122c626", "installed_by": ["fasta_explore_search_plot_tidk"] + }, + "untar": { + "branch": "master", + "git_sha": "e719354ba77df0a1bd310836aa2039b45c29d620", + "installed_by": ["modules"] } } }, diff --git a/modules/local/kraken2.nf b/modules/local/kraken2.nf new file mode 100644 index 00000000..27c7e17f --- /dev/null +++ b/modules/local/kraken2.nf @@ -0,0 +1,47 @@ +process KRAKEN2 { + tag "${asm_tag}" + label 'process_single' + label 'process_high_memory' + + container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + 'https://depot.galaxyproject.org/singularity/kraken2:2.1.2--pl5321h9f5acd7_2': + 'biocontainers/kraken2:2.1.2--pl5321h9f5acd7_2' }" + + input: + tuple val(asm_tag), path(fasta_file) + path db_path + + output: + tuple val(asm_tag), path("*.kraken2.cut"), path("*.kraken2.report") , emit: report + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + """ + kraken2 \\ + --output "${asm_tag}.kraken2.cut" \\ + --report "${asm_tag}.kraken2.report" \\ + --use-names \\ + --db $db_path \\ + --threads ${task.cpus} \\ + $fasta_file > kraken2.log + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + kraken2: \$(echo \$(kraken2 --version 2>&1) | sed 's/^.*Kraken version //; s/ .*\$//') + END_VERSIONS + """ + + stub: + """ + touch "${asm_tag}.kraken2.cut" + touch "${asm_tag}.kraken2.report" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + kraken2: \$(echo \$(kraken2 --version 2>&1) | sed 's/^.*Kraken version //; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/local/kraken2_krona_plot.nf b/modules/local/kraken2_krona_plot.nf new file mode 100644 index 00000000..c4d73275 --- /dev/null +++ b/modules/local/kraken2_krona_plot.nf @@ -0,0 +1,27 @@ +process KRAKEN2_KRONA_PLOT { + tag "${hap_name}" + label 'process_single' + + container "docker.io/nanozoo/krona:2.7.1--e7615f7" + + input: + tuple val(hap_name), path(kraken2_cut), path(kraken2_report) + + output: + tuple path("*.kraken2.krona.cut"), path("*.kraken2.krona.html") , emit: plot + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + """ + perl -lane '@a=split /\\t/; if (\$a[2] =~ /taxid\\s+(\\d+)/) {print "\$a[1]\\t\$1\\t1\\t\$a[3]";}' $kraken2_cut > "${hap_name}.kraken2.krona.cut" + ktImportTaxonomy -i -o "${hap_name}.kraken2.krona.html" -m "4" "${hap_name}.kraken2.krona.cut" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + KronaTools: \$(ktImportTaxonomy | sed -n '/KronaTools/s/KronaTools//p' | tr -d ' _/[:space:]' | sed 's/-ktImportTaxonomy\\\\//1') + END_VERSIONS + """ +} diff --git a/modules/nf-core/untar/environment.yml b/modules/nf-core/untar/environment.yml new file mode 100644 index 00000000..0c9cbb10 --- /dev/null +++ b/modules/nf-core/untar/environment.yml @@ -0,0 +1,11 @@ +name: untar + +channels: + - conda-forge + - bioconda + - defaults + +dependencies: + - conda-forge::grep=3.11 + - conda-forge::sed=4.7 + - conda-forge::tar=1.34 diff --git a/modules/nf-core/untar/main.nf b/modules/nf-core/untar/main.nf new file mode 100644 index 00000000..8a75bb95 --- /dev/null +++ b/modules/nf-core/untar/main.nf @@ -0,0 +1,63 @@ +process UNTAR { + tag "$archive" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : + 'nf-core/ubuntu:20.04' }" + + input: + tuple val(meta), path(archive) + + output: + tuple val(meta), path("$prefix"), emit: untar + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + prefix = task.ext.prefix ?: ( meta.id ? "${meta.id}" : archive.baseName.toString().replaceFirst(/\.tar$/, "")) + + """ + mkdir $prefix + + ## Ensures --strip-components only applied when top level of tar contents is a directory + ## If just files or multiple directories, place all in prefix + if [[ \$(tar -taf ${archive} | grep -o -P "^.*?\\/" | uniq | wc -l) -eq 1 ]]; then + tar \\ + -C $prefix --strip-components 1 \\ + -xavf \\ + $args \\ + $archive \\ + $args2 + else + tar \\ + -C $prefix \\ + -xavf \\ + $args \\ + $archive \\ + $args2 + fi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + untar: \$(echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//') + END_VERSIONS + """ + + stub: + prefix = task.ext.prefix ?: ( meta.id ? "${meta.id}" : archive.toString().replaceFirst(/\.[^\.]+(.gz)?$/, "")) + """ + mkdir $prefix + touch ${prefix}/file.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + untar: \$(echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/untar/meta.yml b/modules/nf-core/untar/meta.yml new file mode 100644 index 00000000..a9a2110f --- /dev/null +++ b/modules/nf-core/untar/meta.yml @@ -0,0 +1,46 @@ +name: untar +description: Extract files. +keywords: + - untar + - uncompress + - extract +tools: + - untar: + description: | + Extract tar.gz files. + documentation: https://www.gnu.org/software/tar/manual/ + licence: ["GPL-3.0-or-later"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - archive: + type: file + description: File to be untar + pattern: "*.{tar}.{gz}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - untar: + type: directory + description: Directory containing contents of archive + pattern: "*/" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@joseespinosa" + - "@drpatelh" + - "@matthdsm" + - "@jfy133" +maintainers: + - "@joseespinosa" + - "@drpatelh" + - "@matthdsm" + - "@jfy133" diff --git a/modules/nf-core/untar/tests/main.nf.test b/modules/nf-core/untar/tests/main.nf.test new file mode 100644 index 00000000..679e83c7 --- /dev/null +++ b/modules/nf-core/untar/tests/main.nf.test @@ -0,0 +1,55 @@ +nextflow_process { + + name "Test Process UNTAR" + script "../main.nf" + process "UNTAR" + + tag "modules" + tag "modules_nfcore" + tag "untar" + + test("test_untar") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ [], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/db/kraken2.tar.gz', checkIfExists: true) ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.untar).match("test_untar") }, + ) + } + + } + + test("test_untar_onlyfiles") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ [], file(params.modules_testdata_base_path + 'generic/tar/hello.tar.gz', checkIfExists: true) ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.untar).match("test_untar_onlyfiles") }, + ) + } + + } + +} diff --git a/modules/nf-core/untar/tests/main.nf.test.snap b/modules/nf-core/untar/tests/main.nf.test.snap new file mode 100644 index 00000000..ace42576 --- /dev/null +++ b/modules/nf-core/untar/tests/main.nf.test.snap @@ -0,0 +1,34 @@ +{ + "test_untar_onlyfiles": { + "content": [ + [ + [ + [ + + ], + [ + "hello.txt:md5,e59ff97941044f85df5297e1c302d260" + ] + ] + ] + ], + "timestamp": "2023-10-18T11:56:46.878844" + }, + "test_untar": { + "content": [ + [ + [ + [ + + ], + [ + "hash.k2d:md5,8b8598468f54a7087c203ad0190555d9", + "opts.k2d:md5,a033d00cf6759407010b21700938f543", + "taxo.k2d:md5,094d5891cdccf2f1468088855c214b2c" + ] + ] + ] + ], + "timestamp": "2023-10-18T11:56:08.16574" + } +} \ No newline at end of file diff --git a/modules/nf-core/untar/tests/tags.yml b/modules/nf-core/untar/tests/tags.yml new file mode 100644 index 00000000..feb6f15c --- /dev/null +++ b/modules/nf-core/untar/tests/tags.yml @@ -0,0 +1,2 @@ +untar: + - modules/nf-core/untar/** diff --git a/subworkflows/local/fasta_kraken2.nf b/subworkflows/local/fasta_kraken2.nf new file mode 100644 index 00000000..fa399a21 --- /dev/null +++ b/subworkflows/local/fasta_kraken2.nf @@ -0,0 +1,44 @@ +include { UNTAR } from '../../modules/nf-core/untar/main' +include { KRAKEN2 } from '../../modules/local/kraken2' +include { KRAKEN2_KRONA_PLOT } from '../../modules/local/kraken2_krona_plot' + +workflow FASTA_KRAKEN2 { + take: + tuple_of_hap_file + db_path // channel: path + + main: + ch_tar_db = db_path + | filter { db -> "$db".endsWith('.tar.gz') } + + ch_untar_db = db_path + | filter { db -> !( "$db".endsWith('.tar.gz') ) } + + // MODULE: UNTAR + UNTAR ( ch_tar_db.map { tar -> [ [ id: "kraken2_db" ], tar ] } ) + + ch_kraken2_inputs = UNTAR.out.untar + | map { meta, untar -> untar } + | mix( + ch_untar_db + ) + | combine(tuple_of_hap_file) + + // MODULE: KRAKEN2 + KRAKEN2( + ch_kraken2_inputs.map { db, tag, fasta -> [ tag, fasta ] }, + ch_kraken2_inputs.map { db, tag, fasta -> db } + ) + + // MODULE: KRAKEN2_KRONA_PLOT + KRAKEN2_KRONA_PLOT ( KRAKEN2.out.report ) + + ch_versions = Channel.empty() + | mix(KRAKEN2.out.versions.first()) + | mix(UNTAR.out.versions.first()) + | mix(KRAKEN2_KRONA_PLOT.out.versions.first()) + + emit: + plot = KRAKEN2_KRONA_PLOT.out.plot + versions = ch_versions +} diff --git a/tests/stub/kraken2/k2_minusb_20231009.tar.gz b/tests/stub/kraken2/k2_minusb_20231009.tar.gz new file mode 100644 index 00000000..e69de29b diff --git a/tests/stub/stub.config b/tests/stub/stub.config index d97be7eb..4f8a1ce5 100644 --- a/tests/stub/stub.config +++ b/tests/stub/stub.config @@ -17,6 +17,9 @@ params { lai_skip = false + kraken2_skip = false + kraken2_db_path = 'tests/stub/kraken2/k2_minusb_20231009.tar.gz' + // Limit resources so that this can run on GitHub Actions max_cpus = 2 max_memory = '6.GB' diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index a6e44f48..f82c3a34 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -34,6 +34,7 @@ include { NCBI_FCS_GX } from '../subworkflows/local/ncbi_f include { ASSEMBLATHON_STATS } from '../modules/local/assemblathon_stats' include { FASTA_BUSCO_PLOT } from '../subworkflows/local/fasta_busco_plot' include { FASTA_LTRRETRIEVER_LAI } from '../subworkflows/pfr/fasta_ltrretriever_lai/main' +include { FASTA_KRAKEN2 } from '../subworkflows/local/fasta_kraken2' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -335,6 +336,22 @@ workflow ASSEMBLYQC { ch_versions = ch_versions.mix(FASTA_LTRRETRIEVER_LAI.out.versions) + // SUBWORKFLOW: FASTA_KRAKEN2 + ch_kraken2_input_assembly = params.kraken2_skip + ? Channel.empty() + : ch_clean_assembly + + ch_kraken2_db_path = params.kraken2_skip + ? Channel.empty() + : Channel.of(file(params.kraken2_db_path, checkIfExists:true)) + FASTA_KRAKEN2( + ch_kraken2_input_assembly, + ch_kraken2_db_path + ) + + ch_kraken2_plot = FASTA_KRAKEN2.out.plot + ch_versions = ch_versions.mix(FASTA_KRAKEN2.out.versions) + // MODULE: CUSTOM_DUMPSOFTWAREVERSIONS CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') From 1589033e65c099db5369c32497c37b7283bc723b Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Fri, 23 Feb 2024 19:01:37 +1300 Subject: [PATCH 26/65] Added fastq_trim_fastp_fastqc --- README.md | 1 - assets/assemblysheet.csv | 4 +- assets/schema_input.json | 17 - modules.json | 15 + modules/nf-core/fastp/environment.yml | 7 + modules/nf-core/fastp/main.nf | 120 +++ modules/nf-core/fastp/meta.yml | 75 ++ modules/nf-core/fastp/tests/main.nf.test | 723 ++++++++++++++++++ modules/nf-core/fastp/tests/main.nf.test.snap | 330 ++++++++ modules/nf-core/fastp/tests/nextflow.config | 6 + modules/nf-core/fastp/tests/tags.yml | 2 + modules/nf-core/fastqc/environment.yml | 7 + modules/nf-core/fastqc/main.nf | 55 ++ modules/nf-core/fastqc/meta.yml | 57 ++ modules/nf-core/fastqc/tests/main.nf.test | 212 +++++ .../nf-core/fastqc/tests/main.nf.test.snap | 88 +++ modules/nf-core/fastqc/tests/tags.yml | 2 + nextflow.config | 2 +- nextflow_schema.json | 9 +- .../nf-core/fastq_trim_fastp_fastqc/main.nf | 106 +++ .../nf-core/fastq_trim_fastp_fastqc/meta.yml | 108 +++ tests/stub/assemblysheet.csv | 4 +- tests/stub/stub.config | 2 + workflows/assemblyqc.nf | 32 +- 24 files changed, 1954 insertions(+), 30 deletions(-) create mode 100644 modules/nf-core/fastp/environment.yml create mode 100644 modules/nf-core/fastp/main.nf create mode 100644 modules/nf-core/fastp/meta.yml create mode 100644 modules/nf-core/fastp/tests/main.nf.test create mode 100644 modules/nf-core/fastp/tests/main.nf.test.snap create mode 100644 modules/nf-core/fastp/tests/nextflow.config create mode 100644 modules/nf-core/fastp/tests/tags.yml create mode 100644 modules/nf-core/fastqc/environment.yml create mode 100644 modules/nf-core/fastqc/main.nf create mode 100644 modules/nf-core/fastqc/meta.yml create mode 100644 modules/nf-core/fastqc/tests/main.nf.test create mode 100644 modules/nf-core/fastqc/tests/main.nf.test.snap create mode 100644 modules/nf-core/fastqc/tests/tags.yml create mode 100644 subworkflows/nf-core/fastq_trim_fastp_fastqc/main.nf create mode 100644 subworkflows/nf-core/fastq_trim_fastp_fastqc/meta.yml diff --git a/README.md b/README.md index 79ec186f..a8c9da60 100644 --- a/README.md +++ b/README.md @@ -73,7 +73,6 @@ Prepare an `assemblysheet.csv` file with following columns representing target a - `fasta:` FASTA file - `gff3 [Optional]:` GFF3 annotation file if available - `monoploid_ids [Optional]:` A txt file listing the IDs used to calculate LAI in monoploid mode if necessary -- `hic_reads [Optional]:` A SRA id such as 'SRR8238190' or path to paired reads such as 'PG_PETUNIA_HiC_CGYCF_CACTCA_L001_R{1,2}.fastq.gz' - `synteny_labels [Optional]:` A two column tsv file listing fasta sequence ids (first column) and labels for the synteny plots (second column) when performing synteny analysis Now, you can run the pipeline using: diff --git a/assets/assemblysheet.csv b/assets/assemblysheet.csv index d8801b5f..19333351 100644 --- a/assets/assemblysheet.csv +++ b/assets/assemblysheet.csv @@ -1,2 +1,2 @@ -tag,fasta,gff3,monoploid_ids,hic_reads,synteny_labels -FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz,,, +tag,fasta,gff3,monoploid_ids,synteny_labels +FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz,, diff --git a/assets/schema_input.json b/assets/schema_input.json index 88f9a89b..981c7786 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -35,23 +35,6 @@ } ] }, - "hic_reads": { - "errorMessage": "HiC reads should either be provided as a SRA ID or as a path to paired reads with pattern '*R{1,2}.(fastq|fq).gz'", - "anyOf": [ - { - "type": "string", - "pattern": "^SR\\w+$" - }, - { - "type": "string", - "pattern": "^\\S+R\\{1,2\\}\\.f(ast)?q\\.gz$" - }, - { - "type": "string", - "maxLength": 0 - } - ] - }, "synteny_labels": { "errorMessage": "Synteny labels tsv path cannot contain spaces and must have extension '.tsv'", "anyOf": [ diff --git a/modules.json b/modules.json index d9161f6a..d68ea0ee 100644 --- a/modules.json +++ b/modules.json @@ -90,6 +90,16 @@ "git_sha": "89ff95427f695086369d7927a3c17cea2a37a382", "installed_by": ["modules"] }, + "fastp": { + "branch": "master", + "git_sha": "003920c7f9a8ae19b69a97171922880220bedf56", + "installed_by": ["fastq_trim_fastp_fastqc"] + }, + "fastqc": { + "branch": "master", + "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", + "installed_by": ["fastq_trim_fastp_fastqc"] + }, "gunzip": { "branch": "master", "git_sha": "3a5fef109d113b4997c9822198664ca5f2716208", @@ -133,6 +143,11 @@ "branch": "master", "git_sha": "2b21fbeb20ad9f17612f4a3dd7b12971513f08d5", "installed_by": ["subworkflows"] + }, + "fastq_trim_fastp_fastqc": { + "branch": "master", + "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", + "installed_by": ["subworkflows"] } } } diff --git a/modules/nf-core/fastp/environment.yml b/modules/nf-core/fastp/environment.yml new file mode 100644 index 00000000..70389e66 --- /dev/null +++ b/modules/nf-core/fastp/environment.yml @@ -0,0 +1,7 @@ +name: fastp +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::fastp=0.23.4 diff --git a/modules/nf-core/fastp/main.nf b/modules/nf-core/fastp/main.nf new file mode 100644 index 00000000..2a3b679e --- /dev/null +++ b/modules/nf-core/fastp/main.nf @@ -0,0 +1,120 @@ +process FASTP { + tag "$meta.id" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/fastp:0.23.4--h5f740d0_0' : + 'biocontainers/fastp:0.23.4--h5f740d0_0' }" + + input: + tuple val(meta), path(reads) + path adapter_fasta + val save_trimmed_fail + val save_merged + + output: + tuple val(meta), path('*.fastp.fastq.gz') , optional:true, emit: reads + tuple val(meta), path('*.json') , emit: json + tuple val(meta), path('*.html') , emit: html + tuple val(meta), path('*.log') , emit: log + path "versions.yml" , emit: versions + tuple val(meta), path('*.fail.fastq.gz') , optional:true, emit: reads_fail + tuple val(meta), path('*.merged.fastq.gz'), optional:true, emit: reads_merged + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def adapter_list = adapter_fasta ? "--adapter_fasta ${adapter_fasta}" : "" + def fail_fastq = save_trimmed_fail && meta.single_end ? "--failed_out ${prefix}.fail.fastq.gz" : save_trimmed_fail && !meta.single_end ? "--unpaired1 ${prefix}_1.fail.fastq.gz --unpaired2 ${prefix}_2.fail.fastq.gz" : '' + // Added soft-links to original fastqs for consistent naming in MultiQC + // Use single ended for interleaved. Add --interleaved_in in config. + if ( task.ext.args?.contains('--interleaved_in') ) { + """ + [ ! -f ${prefix}.fastq.gz ] && ln -sf $reads ${prefix}.fastq.gz + + fastp \\ + --stdout \\ + --in1 ${prefix}.fastq.gz \\ + --thread $task.cpus \\ + --json ${prefix}.fastp.json \\ + --html ${prefix}.fastp.html \\ + $adapter_list \\ + $fail_fastq \\ + $args \\ + 2> >(tee ${prefix}.fastp.log >&2) \\ + | gzip -c > ${prefix}.fastp.fastq.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g") + END_VERSIONS + """ + } else if (meta.single_end) { + """ + [ ! -f ${prefix}.fastq.gz ] && ln -sf $reads ${prefix}.fastq.gz + + fastp \\ + --in1 ${prefix}.fastq.gz \\ + --out1 ${prefix}.fastp.fastq.gz \\ + --thread $task.cpus \\ + --json ${prefix}.fastp.json \\ + --html ${prefix}.fastp.html \\ + $adapter_list \\ + $fail_fastq \\ + $args \\ + 2> >(tee ${prefix}.fastp.log >&2) + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g") + END_VERSIONS + """ + } else { + def merge_fastq = save_merged ? "-m --merged_out ${prefix}.merged.fastq.gz" : '' + """ + [ ! -f ${prefix}_1.fastq.gz ] && ln -sf ${reads[0]} ${prefix}_1.fastq.gz + [ ! -f ${prefix}_2.fastq.gz ] && ln -sf ${reads[1]} ${prefix}_2.fastq.gz + fastp \\ + --in1 ${prefix}_1.fastq.gz \\ + --in2 ${prefix}_2.fastq.gz \\ + --out1 ${prefix}_1.fastp.fastq.gz \\ + --out2 ${prefix}_2.fastp.fastq.gz \\ + --json ${prefix}.fastp.json \\ + --html ${prefix}.fastp.html \\ + $adapter_list \\ + $fail_fastq \\ + $merge_fastq \\ + --thread $task.cpus \\ + --detect_adapter_for_pe \\ + $args \\ + 2> >(tee ${prefix}.fastp.log >&2) + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g") + END_VERSIONS + """ + } + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + def is_single_output = task.ext.args?.contains('--interleaved_in') || meta.single_end + def touch_reads = is_single_output ? "${prefix}.fastp.fastq.gz" : "${prefix}_1.fastp.fastq.gz ${prefix}_2.fastp.fastq.gz" + def touch_merged = (!is_single_output && save_merged) ? "touch ${prefix}.merged.fastq.gz" : "" + """ + touch $touch_reads + touch "${prefix}.fastp.json" + touch "${prefix}.fastp.html" + touch "${prefix}.fastp.log" + $touch_merged + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g") + END_VERSIONS + """ +} diff --git a/modules/nf-core/fastp/meta.yml b/modules/nf-core/fastp/meta.yml new file mode 100644 index 00000000..c22a16ab --- /dev/null +++ b/modules/nf-core/fastp/meta.yml @@ -0,0 +1,75 @@ +name: fastp +description: Perform adapter/quality trimming on sequencing reads +keywords: + - trimming + - quality control + - fastq +tools: + - fastp: + description: | + A tool designed to provide fast all-in-one preprocessing for FastQ files. This tool is developed in C++ with multithreading supported to afford high performance. + documentation: https://github.com/OpenGene/fastp + doi: 10.1093/bioinformatics/bty560 + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information. Use 'single_end: true' to specify single ended or interleaved FASTQs. Use 'single_end: false' for paired-end reads. + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. If you wish to run interleaved paired-end data, supply as single-end data + but with `--interleaved_in` in your `modules.conf`'s `ext.args` for the module. + - adapter_fasta: + type: file + description: File in FASTA format containing possible adapters to remove. + pattern: "*.{fasta,fna,fas,fa}" + - save_trimmed_fail: + type: boolean + description: Specify true to save files that failed to pass trimming thresholds ending in `*.fail.fastq.gz` + - save_merged: + type: boolean + description: Specify true to save all merged reads to the a file ending in `*.merged.fastq.gz` +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: The trimmed/modified/unmerged fastq reads + pattern: "*fastp.fastq.gz" + - json: + type: file + description: Results in JSON format + pattern: "*.json" + - html: + type: file + description: Results in HTML format + pattern: "*.html" + - log: + type: file + description: fastq log file + pattern: "*.log" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - reads_fail: + type: file + description: Reads the failed the preprocessing + pattern: "*fail.fastq.gz" + - reads_merged: + type: file + description: Reads that were successfully merged + pattern: "*.{merged.fastq.gz}" +authors: + - "@drpatelh" + - "@kevinmenden" +maintainers: + - "@drpatelh" + - "@kevinmenden" diff --git a/modules/nf-core/fastp/tests/main.nf.test b/modules/nf-core/fastp/tests/main.nf.test new file mode 100644 index 00000000..9b3f9a38 --- /dev/null +++ b/modules/nf-core/fastp/tests/main.nf.test @@ -0,0 +1,723 @@ +nextflow_process { + + name "Test Process FASTP" + script "../main.nf" + process "FASTP" + tag "modules" + tag "modules_nfcore" + tag "fastp" + + test("test_fastp_single_end") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = false + save_merged = false + + input[0] = Channel.of([ + [ id:'test', single_end:true ], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + def html_text = [ "Q20 bases:

    ") }, + + { assert snapshot(process.out.versions).match("fastqc_versions_single") } + ) + } + } + + test("sarscov2 paired-end [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("") }, + { assert path(process.out.html[0][1][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("fastqc_versions_paired") } + ) + } + } + + test("sarscov2 interleaved [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") } + ) + } + } + + test("sarscov2 paired-end [bam]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("fastqc_versions_bam") } + ) + } + } + + test("sarscov2 multiple [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, + { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, + { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("") }, + { assert path(process.out.html[0][1][1]).text.contains("") }, + { assert path(process.out.html[0][1][2]).text.contains("") }, + { assert path(process.out.html[0][1][3]).text.contains("") }, + + { assert snapshot(process.out.versions).match("fastqc_versions_multiple") } + ) + } + } + + test("sarscov2 custom_prefix") { + + when { + process { + """ + input[0] = Channel.of([ + [ id:'mysample', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") } + ) + } + } + + test("sarscov2 single-end [fastq] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of([ + [ id: 'test', single_end:true ], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.html.collect { file(it[1]).getName() } + + process.out.zip.collect { file(it[1]).getName() } + + process.out.versions ).match("fastqc_stub") } + ) + } + } + +} diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap new file mode 100644 index 00000000..86f7c311 --- /dev/null +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -0,0 +1,88 @@ +{ + "fastqc_versions_interleaved": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:07.293713" + }, + "fastqc_stub": { + "content": [ + [ + "test.html", + "test.zip", + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:31:01.425198" + }, + "fastqc_versions_multiple": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:55.797907" + }, + "fastqc_versions_bam": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:26.795862" + }, + "fastqc_versions_single": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:27.043675" + }, + "fastqc_versions_paired": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:47.584191" + }, + "fastqc_versions_custom_prefix": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:41:14.576531" + } +} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/tags.yml b/modules/nf-core/fastqc/tests/tags.yml new file mode 100644 index 00000000..7834294b --- /dev/null +++ b/modules/nf-core/fastqc/tests/tags.yml @@ -0,0 +1,2 @@ +fastqc: + - modules/nf-core/fastqc/** diff --git a/nextflow.config b/nextflow.config index ec565e3c..cf50c3f7 100644 --- a/nextflow.config +++ b/nextflow.config @@ -43,7 +43,7 @@ params { kraken2_db_path = null // HiC options - hic_skip = true + hic = null hic_skip_fastp = false hic_skip_fastqc = false hic_fastp_ext_args = '--qualified_quality_phred 20 --length_required 50' diff --git a/nextflow_schema.json b/nextflow_schema.json index add593cc..fcbfcbd1 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -183,10 +183,11 @@ "description": "", "default": "", "properties": { - "hic_skip": { - "type": "boolean", - "default": true, - "description": "Skip HiC contact map construction" + "hic": { + "type": "string", + "description": "HiC reads", + "help_text": "Path to reads provided as a SRA ID or as a path to paired reads with pattern '*R{1,2}.(fastq|fq).gz'", + "pattern": "^SR\\w+$|^\\S+R\\{1,2\\}\\.f(ast)?q\\.gz$" }, "hic_skip_fastp": { "type": "boolean", diff --git a/subworkflows/nf-core/fastq_trim_fastp_fastqc/main.nf b/subworkflows/nf-core/fastq_trim_fastp_fastqc/main.nf new file mode 100644 index 00000000..39a086ad --- /dev/null +++ b/subworkflows/nf-core/fastq_trim_fastp_fastqc/main.nf @@ -0,0 +1,106 @@ +// +// Read QC and trimming +// + +include { FASTQC as FASTQC_RAW } from '../../../modules/nf-core/fastqc/main' +include { FASTQC as FASTQC_TRIM } from '../../../modules/nf-core/fastqc/main' +include { FASTP } from '../../../modules/nf-core/fastp/main' + +// +// Function that parses fastp json output file to get total number of reads after trimming +// +import groovy.json.JsonSlurper + +def getFastpReadsAfterFiltering(json_file) { + def Map json = (Map) new JsonSlurper().parseText(json_file.text).get('summary') + return json['after_filtering']['total_reads'].toLong() +} + +workflow FASTQ_TRIM_FASTP_FASTQC { + take: + ch_reads // channel: [ val(meta), path(reads) ] + ch_adapter_fasta // channel: [ path(fasta) ] + val_save_trimmed_fail // value: boolean + val_save_merged // value: boolean + val_skip_fastp // value: boolean + val_skip_fastqc // value: boolean + + main: + + ch_versions = Channel.empty() + + ch_fastqc_raw_html = Channel.empty() + ch_fastqc_raw_zip = Channel.empty() + if (!val_skip_fastqc) { + FASTQC_RAW ( + ch_reads + ) + ch_fastqc_raw_html = FASTQC_RAW.out.html + ch_fastqc_raw_zip = FASTQC_RAW.out.zip + ch_versions = ch_versions.mix(FASTQC_RAW.out.versions.first()) + } + + ch_trim_reads = ch_reads + ch_trim_json = Channel.empty() + ch_trim_html = Channel.empty() + ch_trim_log = Channel.empty() + ch_trim_reads_fail = Channel.empty() + ch_trim_reads_merged = Channel.empty() + ch_fastqc_trim_html = Channel.empty() + ch_fastqc_trim_zip = Channel.empty() + if (!val_skip_fastp) { + FASTP ( + ch_reads, + ch_adapter_fasta, + val_save_trimmed_fail, + val_save_merged + ) + ch_trim_reads = FASTP.out.reads + ch_trim_json = FASTP.out.json + ch_trim_html = FASTP.out.html + ch_trim_log = FASTP.out.log + ch_trim_reads_fail = FASTP.out.reads_fail + ch_trim_reads_merged = FASTP.out.reads_merged + ch_versions = ch_versions.mix(FASTP.out.versions.first()) + + // + // Filter empty FastQ files after adapter trimming so FastQC doesn't fail + // + ch_trim_reads + .join(ch_trim_json) + .map { meta, reads, json -> + if (json.text.readLines().size < 1) { + return [ meta, reads ] + } + + if (getFastpReadsAfterFiltering(json) > 0) { + [ meta, reads ] + } + } + .set { ch_trim_reads } + + if (!val_skip_fastqc) { + FASTQC_TRIM ( + ch_trim_reads + ) + ch_fastqc_trim_html = FASTQC_TRIM.out.html + ch_fastqc_trim_zip = FASTQC_TRIM.out.zip + ch_versions = ch_versions.mix(FASTQC_TRIM.out.versions.first()) + } + } + + emit: + reads = ch_trim_reads // channel: [ val(meta), path(reads) ] + trim_json = ch_trim_json // channel: [ val(meta), path(json) ] + trim_html = ch_trim_html // channel: [ val(meta), path(html) ] + trim_log = ch_trim_log // channel: [ val(meta), path(log) ] + trim_reads_fail = ch_trim_reads_fail // channel: [ val(meta), path(fastq.gz) ] + trim_reads_merged = ch_trim_reads_merged // channel: [ val(meta), path(fastq.gz) ] + + fastqc_raw_html = ch_fastqc_raw_html // channel: [ val(meta), path(html) ] + fastqc_raw_zip = ch_fastqc_raw_zip // channel: [ val(meta), path(zip) ] + fastqc_trim_html = ch_fastqc_trim_html // channel: [ val(meta), path(html) ] + fastqc_trim_zip = ch_fastqc_trim_zip // channel: [ val(meta), path(zip) ] + + versions = ch_versions.ifEmpty(null) // channel: [ path(versions.yml) ] +} diff --git a/subworkflows/nf-core/fastq_trim_fastp_fastqc/meta.yml b/subworkflows/nf-core/fastq_trim_fastp_fastqc/meta.yml new file mode 100644 index 00000000..9f4e12e0 --- /dev/null +++ b/subworkflows/nf-core/fastq_trim_fastp_fastqc/meta.yml @@ -0,0 +1,108 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "fastq_trim_fastp_fastqc" +description: Read QC, fastp trimming and read qc +keywords: + - qc + - quality_control + - adapters + - trimming + - fastq +components: + - fastqc + - fastp +input: + - ch_reads: + type: file + description: | + Structure: [ val(meta), path (reads) ] + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ], List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. If you wish to run interleaved paired-end data, supply as single-end data + but with `--interleaved_in` in your `modules.conf`'s `ext.args` for the module. + - ch_adapter_fasta: + type: file + description: | + Structure: path(adapter_fasta) + File in FASTA format containing possible adapters to remove. + - val_save_trimmed_fail: + type: boolean + description: | + Structure: val(save_trimmed_fail) + Specify true to save files that failed to pass trimming thresholds ending in `*.fail.fastq.gz` + - val_save_merged: + type: boolean + description: | + Structure: val(save_merged) + Specify true to save all merged reads to the a file ending in `*.merged.fastq.gz` + - val_skip_fastqc: + type: boolean + description: | + Structure: val(skip_fastqc) + skip the fastqc process if true + - val_skip_fastp: + type: boolean + description: | + Structure: val(skip_fastp) + skip the fastp process if true +output: + - meta: + type: value + description: Groovy Map containing sample information e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + Structure: [ val(meta), path(reads) ] + The trimmed/modified/unmerged fastq reads + - trim_json: + type: file + description: | + Structure: [ val(meta), path(trim_json) ] + Results in JSON format + - trim_html: + type: file + description: | + Structure: [ val(meta), path(trim_html) ] + Results in HTML format + - trim_log: + type: file + description: | + Structure: [ val(meta), path(trim_log) ] + fastq log file + - trim_reads_fail: + type: file + description: | + Structure: [ val(meta), path(trim_reads_fail) ] + Reads the failed the preprocessing + - trim_reads_merged: + type: file + description: | + Structure: [ val(meta), path(trim_reads_merged) ] + Reads that were successfully merged + - fastqc_raw_html: + type: file + description: | + Structure: [ val(meta), path(fastqc_raw_html) ] + Raw fastQC report + - fastqc_raw_zip: + type: file + description: | + Structure: [ val(meta), path(fastqc_raw_zip) ] + Raw fastQC report archive + - fastqc_trim_html: + type: file + description: | + Structure: [ val(meta), path(fastqc_trim_html) ] + Trimmed fastQC report + - fastqc_trim_zip: + type: file + description: | + Structure: [ val(meta), path(fastqc_trim_zip) ] + Trimmed fastQC report archive + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@Joon-Klaps" +maintainers: + - "@Joon-Klaps" diff --git a/tests/stub/assemblysheet.csv b/tests/stub/assemblysheet.csv index dd67ad3b..69b0eb4f 100644 --- a/tests/stub/assemblysheet.csv +++ b/tests/stub/assemblysheet.csv @@ -1,2 +1,2 @@ -tag,fasta,gff3,monoploid_ids,hic_reads,synteny_labels -FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz,https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/tests/stub/FI1.monoploid.seqs.txt,"https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/docs/test_files/hic/stub_hic.R{1,2}.fq.gz",https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/FI1.seq.labels.tsv +tag,fasta,gff3,monoploid_ids,synteny_labels +FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz,https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/tests/stub/FI1.monoploid.seqs.txt,https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/FI1.seq.labels.tsv diff --git a/tests/stub/stub.config b/tests/stub/stub.config index 4f8a1ce5..5664ccc0 100644 --- a/tests/stub/stub.config +++ b/tests/stub/stub.config @@ -20,6 +20,8 @@ params { kraken2_skip = false kraken2_db_path = 'tests/stub/kraken2/k2_minusb_20231009.tar.gz' + hic = 'tests/stub/hic/Dummy_hic.R{1,2}.fq.gz' + // Limit resources so that this can run on GitHub Actions max_cpus = 2 max_memory = '6.GB' diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index f82c3a34..48e3c7d2 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -50,6 +50,7 @@ include { GUNZIP as GUNZIP_FASTA } from '../modules/nf-core/gunzip/ma include { GUNZIP as GUNZIP_GFF3 } from '../modules/nf-core/gunzip/main' include { FASTAVALIDATOR } from '../modules/nf-core/fastavalidator/main' include { FASTA_EXPLORE_SEARCH_PLOT_TIDK } from '../subworkflows/nf-core/fasta_explore_search_plot_tidk/main' +include { FASTQ_TRIM_FASTP_FASTQC } from '../subworkflows/nf-core/fastq_trim_fastp_fastqc/main' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' @@ -70,7 +71,7 @@ workflow ASSEMBLYQC { ch_input = Channel.fromSamplesheet('input') ch_target_assemby_branch = ch_input - | map { tag, fasta, gff, mono_ids, reads, labels -> + | map { tag, fasta, gff, mono_ids, labels -> [ [ id: tag ], file(fasta, checkIfExists: true) ] } | branch { meta, fasta -> @@ -79,7 +80,7 @@ workflow ASSEMBLYQC { } ch_assemby_gff3_branch = ch_input - | map { tag, fasta, gff, mono_ids, reads, labels -> + | map { tag, fasta, gff, mono_ids, labels -> gff ? [ [ id: tag ], file(gff, checkIfExists: true) ] : null @@ -90,12 +91,23 @@ workflow ASSEMBLYQC { } ch_mono_ids = ch_input - | map { tag, fasta, gff, mono_ids, reads, labels -> + | map { tag, fasta, gff, mono_ids, labels -> mono_ids ? [ [ id: tag ], file(mono_ids, checkIfExists: true) ] : null } + ch_hic_reads = !params.hic + ? Channel.empty() + : ( + "$params.hic".find(/.*[\/].*\.(fastq|fq)\.gz/) + ? Channel.fromFilePairs(params.hic, checkIfExists: true) + : Channel.fromSRA(params.hic) + ) + | map{ sample, fq -> + [ [ id: sample, single_end: false ], fq ] + } + // MODULE: GUNZIP as GUNZIP_FASTA GUNZIP_FASTA ( ch_target_assemby_branch.gz ) @@ -352,6 +364,20 @@ workflow ASSEMBLYQC { ch_kraken2_plot = FASTA_KRAKEN2.out.plot ch_versions = ch_versions.mix(FASTA_KRAKEN2.out.versions) + // SUBWORKFLOW: FASTQ_TRIM_FASTP_FASTQC + + FASTQ_TRIM_FASTP_FASTQC( + ch_hic_reads, + [], + true, // val_save_trimmed_fail + false, // val_save_merged + params.hic_skip_fastp, + params.hic_skip_fastqc + ) + + ch_cleaned_paired_reads = FASTQ_TRIM_FASTP_FASTQC.out.reads + ch_versions = ch_versions.mix(FASTQ_TRIM_FASTP_FASTQC.out.versions) + // MODULE: CUSTOM_DUMPSOFTWAREVERSIONS CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') From f0bc5a6691d6630b31061238dbdb226840efe1df Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 26 Feb 2024 14:41:46 +1300 Subject: [PATCH 27/65] Added FQ2HIC subworkflow --- bin/assembly2bedpe.py | 47 +++++ bin/hic2html.py | 24 +++ .../templates/hic/hic_html_template.html | 92 ++++++++++ conf/base.config | 6 + modules.json | 20 ++ modules/local/agp2assembly.nf | 19 ++ modules/local/assembly2bedpe.nf | 25 +++ modules/local/hic2html.nf | 19 ++ modules/local/hicqc.nf | 37 ++++ modules/local/juicer_sort.nf | 22 +++ modules/local/makeagpfromfasta.nf | 18 ++ modules/local/matlock_bam2_juicer.nf | 19 ++ modules/local/runassemblyvisualizer.nf | 31 ++++ modules/pfr/bwa/index/environment.yml | 7 + modules/pfr/bwa/index/main.nf | 53 ++++++ modules/pfr/bwa/index/meta.yml | 45 +++++ modules/pfr/bwa/index/tests/main.nf.test | 33 ++++ modules/pfr/bwa/index/tests/main.nf.test.snap | 43 +++++ modules/pfr/bwa/index/tests/tags.yml | 2 + modules/pfr/bwa/mem/environment.yml | 10 + modules/pfr/bwa/mem/main.nf | 55 ++++++ modules/pfr/bwa/mem/meta.yml | 58 ++++++ modules/pfr/bwa/mem/tests/main.nf.test | 173 ++++++++++++++++++ modules/pfr/bwa/mem/tests/main.nf.test.snap | 142 ++++++++++++++ modules/pfr/bwa/mem/tests/tags.yml | 3 + modules/pfr/samblaster/environment.yml | 9 + modules/pfr/samblaster/main.nf | 50 +++++ modules/pfr/samblaster/meta.yml | 53 ++++++ subworkflows/local/fq2hic.nf | 79 ++++++++ .../pfr/fastq_bwa_mem_samblaster/main.nf | 61 ++++++ .../pfr/fastq_bwa_mem_samblaster/meta.yml | 52 ++++++ .../tests/main.nf.test | 44 +++++ .../tests/main.nf.test.snap | 41 +++++ .../tests/nextflow.config | 11 ++ .../fastq_bwa_mem_samblaster/tests/tags.yml | 2 + workflows/assemblyqc.nf | 28 +-- 36 files changed, 1420 insertions(+), 13 deletions(-) create mode 100755 bin/assembly2bedpe.py create mode 100755 bin/hic2html.py create mode 100644 bin/report_modules/templates/hic/hic_html_template.html create mode 100644 modules/local/agp2assembly.nf create mode 100644 modules/local/assembly2bedpe.nf create mode 100644 modules/local/hic2html.nf create mode 100644 modules/local/hicqc.nf create mode 100644 modules/local/juicer_sort.nf create mode 100644 modules/local/makeagpfromfasta.nf create mode 100644 modules/local/matlock_bam2_juicer.nf create mode 100644 modules/local/runassemblyvisualizer.nf create mode 100644 modules/pfr/bwa/index/environment.yml create mode 100644 modules/pfr/bwa/index/main.nf create mode 100644 modules/pfr/bwa/index/meta.yml create mode 100644 modules/pfr/bwa/index/tests/main.nf.test create mode 100644 modules/pfr/bwa/index/tests/main.nf.test.snap create mode 100644 modules/pfr/bwa/index/tests/tags.yml create mode 100644 modules/pfr/bwa/mem/environment.yml create mode 100644 modules/pfr/bwa/mem/main.nf create mode 100644 modules/pfr/bwa/mem/meta.yml create mode 100644 modules/pfr/bwa/mem/tests/main.nf.test create mode 100644 modules/pfr/bwa/mem/tests/main.nf.test.snap create mode 100644 modules/pfr/bwa/mem/tests/tags.yml create mode 100644 modules/pfr/samblaster/environment.yml create mode 100644 modules/pfr/samblaster/main.nf create mode 100644 modules/pfr/samblaster/meta.yml create mode 100644 subworkflows/local/fq2hic.nf create mode 100644 subworkflows/pfr/fastq_bwa_mem_samblaster/main.nf create mode 100644 subworkflows/pfr/fastq_bwa_mem_samblaster/meta.yml create mode 100644 subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test create mode 100644 subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test.snap create mode 100644 subworkflows/pfr/fastq_bwa_mem_samblaster/tests/nextflow.config create mode 100644 subworkflows/pfr/fastq_bwa_mem_samblaster/tests/tags.yml diff --git a/bin/assembly2bedpe.py b/bin/assembly2bedpe.py new file mode 100755 index 00000000..94e49681 --- /dev/null +++ b/bin/assembly2bedpe.py @@ -0,0 +1,47 @@ +#!/usr/bin/env python + +import sys +import pandas as pd + + +def read_assembly_file_cols(assembly_file_name): + with open(assembly_file_name, "r") as file: + lines = file.readlines() + + list_of_items = [line.replace("\n", "").split(" ") for line in lines] + list_of_three_tuples = [items for items in list_of_items if len(items) == 3] + list_of_three_tuples_wt = [ + [x[0], int(x[1]), int(x[2])] for x in list_of_three_tuples + ] + + df = pd.DataFrame(list_of_three_tuples_wt) + df.columns = ["name", "number", "length"] + + return df + + +def make_bedpe_cols(assembly_file_pd): + pd = assembly_file_pd + pd["cum_length"] = pd["length"].cumsum() + pd["end_index"] = pd["cum_length"] - 1 + + start_index = pd["end_index"].shift(periods=1, fill_value=-1) + 1 + pd["start_index"] = start_index + + return pd + + +def print_bed_pe_file(bed_pe_df): + df = bed_pe_df + print("chr1\tx1\tx2\tchr2\ty1\ty2\tname\tscore\tstrand1\tstrand2\tcolor") + for index, row in df.iterrows(): + print( + f"assembly\t{row['start_index']}\t{row['end_index']}\tassembly\t{row['start_index']}\t{row['end_index']}\t{row['name'].replace('>', '')}\t.\t.\t.\t0,0,255" + ) + + +if __name__ == "__main__": + assembly_file_name = sys.argv[1] + + assembly_file_cols = read_assembly_file_cols(assembly_file_name) + print_bed_pe_file(make_bedpe_cols(assembly_file_cols)) diff --git a/bin/hic2html.py b/bin/hic2html.py new file mode 100755 index 00000000..eacc08f8 --- /dev/null +++ b/bin/hic2html.py @@ -0,0 +1,24 @@ +#!/usr/bin/env python + +import sys +from pathlib import Path +import os + + +if __name__ == "__main__": + hic_file_name = os.path.basename(sys.argv[1]) + + projectDir = "/".join(__file__.split("/")[0:-1]) + html_template_path = Path( + f"{projectDir}/report_modules/templates/hic/hic_html_template.html" + ) + + with open(html_template_path) as f: + html_file_lines = "".join(f.readlines()) + + filled_template = html_file_lines.replace("HIC_FILE_NAME", hic_file_name).replace( + "BEDPE_FILE_NAME", + f"{hic_file_name.replace('.hic', '')}.assembly.bedpe", + ) + + print(filled_template) diff --git a/bin/report_modules/templates/hic/hic_html_template.html b/bin/report_modules/templates/hic/hic_html_template.html new file mode 100644 index 00000000..6d3f4088 --- /dev/null +++ b/bin/report_modules/templates/hic/hic_html_template.html @@ -0,0 +1,92 @@ + + + + + HiC Contact Map + + + + + + + + +
    +
    +
    + + + diff --git a/conf/base.config b/conf/base.config index e683193a..9f5c47e1 100644 --- a/conf/base.config +++ b/conf/base.config @@ -61,6 +61,12 @@ process { withName:NCBI_FCS_GX_SCREEN_SAMPLES { memory = { check_max( 512.GB * task.attempt, 'memory' ) } } + withName:BWA_MEM { + time = { check_max( 2.day * task.attempt, 'time' ) } + } + withName:SAMBLASTER { + time = { check_max( 20.h * task.attempt, 'time' ) } + } withName:CUSTOM_DUMPSOFTWAREVERSIONS { cache = false } diff --git a/modules.json b/modules.json index d68ea0ee..a57849ee 100644 --- a/modules.json +++ b/modules.json @@ -5,6 +5,16 @@ "git@github.com:PlantandFoodResearch/nxf-modules.git": { "modules": { "pfr": { + "bwa/index": { + "branch": "main", + "git_sha": "4b9da80b1e4c16067babd97554bea42d7cd9ca85", + "installed_by": ["fastq_bwa_mem_samblaster"] + }, + "bwa/mem": { + "branch": "main", + "git_sha": "835879b8f174bb4d2c5534d0381ffbe62cc1b060", + "installed_by": ["fastq_bwa_mem_samblaster"] + }, "cat/cat": { "branch": "main", "git_sha": "4b9da80b1e4c16067babd97554bea42d7cd9ca85", @@ -59,6 +69,11 @@ "branch": "main", "git_sha": "835879b8f174bb4d2c5534d0381ffbe62cc1b060", "installed_by": ["fasta_ltrretriever_lai"] + }, + "samblaster": { + "branch": "main", + "git_sha": "73358a6712178b9a67c39f92e65e8144b5880eae", + "installed_by": ["fastq_bwa_mem_samblaster"] } } }, @@ -69,6 +84,11 @@ "git_sha": "154661d7c1769532ff7b5f11259644ec200dd47d", "installed_by": ["subworkflows"] }, + "fastq_bwa_mem_samblaster": { + "branch": "main", + "git_sha": "9639ac9a556898d0f0e8592bff24585c33326458", + "installed_by": ["subworkflows"] + }, "gff3_validate": { "branch": "main", "git_sha": "f9b96bf8142a01f0649ff90570fb10aa973504b9", diff --git a/modules/local/agp2assembly.nf b/modules/local/agp2assembly.nf new file mode 100644 index 00000000..edb60d96 --- /dev/null +++ b/modules/local/agp2assembly.nf @@ -0,0 +1,19 @@ +process AGP2ASSEMBLY { + tag "$sample_id_on_tag" + label 'process_single' + + container "docker.io/gallvp/juicebox_scripts:a7ae991_ps" + publishDir "${params.outdir}/hic/assembly", mode:'copy' + + input: + tuple val(sample_id_on_tag), path(agp_file) + + output: + tuple val(sample_id_on_tag), path("*.agp.assembly"), emit: assembly + + script: + """ + assembly_tag=\$(echo $sample_id_on_tag | sed 's/.*\\.on\\.//g') + agp2assembly.py $agp_file "\${assembly_tag}.agp.assembly" + """ +} diff --git a/modules/local/assembly2bedpe.nf b/modules/local/assembly2bedpe.nf new file mode 100644 index 00000000..d0b7f6ac --- /dev/null +++ b/modules/local/assembly2bedpe.nf @@ -0,0 +1,25 @@ +process ASSEMBLY2BEDPE { + tag "$sample_id_on_tag" + label 'process_single' + + container "docker.io/gallvp/python3npkgs:v0.4" + publishDir "${params.outdir}/hic/bedpe", mode:'copy' + + input: + tuple val(sample_id_on_tag), path(agp_assembly_file) + + output: + tuple val(sample_id_on_tag), path("*.assembly.bedpe"), emit: bedpe + + script: + """ + assembly_tag=\$(echo $sample_id_on_tag | sed 's/.*\\.on\\.//g') + assembly2bedpe.py $agp_assembly_file > "\${assembly_tag}.assembly.bedpe" + """ + + stub: + """ + assembly_tag=\$(echo $sample_id_on_tag | sed 's/.*\\.on\\.//g') + touch "\${assembly_tag}.assembly.bedpe" + """ +} diff --git a/modules/local/hic2html.nf b/modules/local/hic2html.nf new file mode 100644 index 00000000..ba0df637 --- /dev/null +++ b/modules/local/hic2html.nf @@ -0,0 +1,19 @@ +process HIC2HTML { + tag "$sample_id_on_tag" + label 'process_single' + + container "docker.io/gallvp/python3npkgs:v0.4" + publishDir "${params.outdir}/hic", mode: 'copy' + + input: + tuple val(sample_id_on_tag), path(hic_file) + + output: + path "*.html", emit: html + + script: + """ + file_name="$hic_file" + hic2html.py "$hic_file" > "\${file_name%.*}.html" + """ +} diff --git a/modules/local/hicqc.nf b/modules/local/hicqc.nf new file mode 100644 index 00000000..0f5c740a --- /dev/null +++ b/modules/local/hicqc.nf @@ -0,0 +1,37 @@ +process HICQC { + tag "$meta.id" + label 'process_single' + + publishDir "${params.outdir}/hic/hic_qc", mode:'copy' + container "docker.io/gallvp/hic_qc:6881c33_ps" + + input: + tuple val(meta), path(bam) + + output: + tuple val(meta), path("*.pdf") , emit: pdf + path "versions.yml" , emit: versions + + script: + """ + hic_qc.py \\ + -n 10000000 \\ + -b $bam \\ + --outfile_prefix "$meta.id" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + hic_qc.py: \$(hic_qc.py --version) + END_VERSIONS + """ + + stub: + """ + touch "${meta.id}.pdf" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + hic_qc.py: \$(hic_qc.py --version) + END_VERSIONS + """ +} diff --git a/modules/local/juicer_sort.nf b/modules/local/juicer_sort.nf new file mode 100644 index 00000000..c57124b7 --- /dev/null +++ b/modules/local/juicer_sort.nf @@ -0,0 +1,22 @@ +process JUICER_SORT { + tag "$sample_id_on_tag" + label 'process_high' + + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/ubuntu:20.04': + 'nf-core/ubuntu:20.04' }" + + input: + tuple val(sample_id_on_tag), path(out_links_txt) + + output: + tuple val(sample_id_on_tag), path("*sorted.links.txt"), emit: links + + script: + """ + sort --parallel=${task.cpus} \\ + -k2,2 -k6,6 \\ + $out_links_txt \\ + > out.sorted.links.txt + """ +} diff --git a/modules/local/makeagpfromfasta.nf b/modules/local/makeagpfromfasta.nf new file mode 100644 index 00000000..3c5a0972 --- /dev/null +++ b/modules/local/makeagpfromfasta.nf @@ -0,0 +1,18 @@ +process MAKEAGPFROMFASTA { + tag "$sample_id_on_tag" + label 'process_single' + + container "docker.io/gallvp/juicebox_scripts:a7ae991_ps" + + input: + tuple val(sample_id_on_tag), path(assembly_fasta) + + output: + tuple val(sample_id_on_tag), path("*.agp"), emit: agp + + script: + """ + file_name="$assembly_fasta" + makeAgpFromFasta.py $assembly_fasta "\${file_name%%.*}.agp" + """ +} diff --git a/modules/local/matlock_bam2_juicer.nf b/modules/local/matlock_bam2_juicer.nf new file mode 100644 index 00000000..5222acdf --- /dev/null +++ b/modules/local/matlock_bam2_juicer.nf @@ -0,0 +1,19 @@ +process MATLOCK_BAM2_JUICER { + tag "$sample_id_on_tag" + label 'process_single' + + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/matlock:20181227--h4b03ef3_3': + 'biocontainers/matlock:20181227--h4b03ef3_3' }" + + input: + tuple val(sample_id_on_tag), path(hic_bam_scaffolds) + + output: + tuple val(sample_id_on_tag), path("out.links.txt") + + script: + """ + matlock bam2 juicer $hic_bam_scaffolds out.links.txt + """ +} diff --git a/modules/local/runassemblyvisualizer.nf b/modules/local/runassemblyvisualizer.nf new file mode 100644 index 00000000..a13ed92b --- /dev/null +++ b/modules/local/runassemblyvisualizer.nf @@ -0,0 +1,31 @@ +process RUNASSEMBLYVISUALIZER { + tag "$sample_id_on_tag" + label "process_medium" + + publishDir "${params.outdir}/hic", mode:'copy' + container "docker.io/gallvp/3d-dna:63029aa" + + input: + tuple val(sample_id_on_tag), path(agp_assembly_file), path(sorted_links_txt_file) + + output: + tuple val(sample_id_on_tag), path("*.hic"), emit: hic + + script: + // -p true/false Use GNU Parallel to speed up computation (default is true). + """ + assembly_tag=\$(echo $sample_id_on_tag | sed 's/.*\\.on\\.//g') + file_name="${agp_assembly_file}" + + /usr/src/3d-dna/visualize/run-assembly-visualizer.sh \\ + $agp_assembly_file $sorted_links_txt_file + + mv "\${file_name%.*}.hic" "\${assembly_tag}.hic" + """ + + stub: + """ + assembly_tag=\$(echo $sample_id_on_tag | sed 's/.*\\.on\\.//g') + touch "\${assembly_tag}.hic" + """ +} diff --git a/modules/pfr/bwa/index/environment.yml b/modules/pfr/bwa/index/environment.yml new file mode 100644 index 00000000..5d3cb323 --- /dev/null +++ b/modules/pfr/bwa/index/environment.yml @@ -0,0 +1,7 @@ +name: bwa_index +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bwa=0.7.17 diff --git a/modules/pfr/bwa/index/main.nf b/modules/pfr/bwa/index/main.nf new file mode 100644 index 00000000..24b5a2ea --- /dev/null +++ b/modules/pfr/bwa/index/main.nf @@ -0,0 +1,53 @@ +process BWA_INDEX { + tag "$fasta" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bwa:0.7.17--hed695b0_7' : + 'biocontainers/bwa:0.7.17--hed695b0_7' }" + + input: + tuple val(meta), path(fasta) + + output: + tuple val(meta), path(bwa) , emit: index + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def prefix = task.ext.prefix ?: "${fasta.baseName}" + def args = task.ext.args ?: '' + """ + mkdir bwa + bwa \\ + index \\ + $args \\ + -p bwa/${prefix} \\ + $fasta + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bwa: \$(echo \$(bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${fasta.baseName}" + """ + mkdir bwa + + touch bwa/${prefix}.amb + touch bwa/${prefix}.ann + touch bwa/${prefix}.bwt + touch bwa/${prefix}.pac + touch bwa/${prefix}.sa + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bwa: \$(echo \$(bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//') + END_VERSIONS + """ +} diff --git a/modules/pfr/bwa/index/meta.yml b/modules/pfr/bwa/index/meta.yml new file mode 100644 index 00000000..730628d0 --- /dev/null +++ b/modules/pfr/bwa/index/meta.yml @@ -0,0 +1,45 @@ +name: bwa_index +description: Create BWA index for reference genome +keywords: + - index + - fasta + - genome + - reference +tools: + - bwa: + description: | + BWA is a software package for mapping DNA sequences against + a large reference genome, such as the human genome. + homepage: http://bio-bwa.sourceforge.net/ + documentation: http://www.htslib.org/doc/samtools.html + arxiv: arXiv:1303.3997 + licence: ["GPL-3.0-or-later"] +input: + - meta: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test', single_end:false ] + - fasta: + type: file + description: Input genome fasta file +output: + - meta: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test', single_end:false ] + - index: + type: file + description: BWA genome index files + pattern: "*.{amb,ann,bwt,pac,sa}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" + - "@maxulysse" +maintainers: + - "@drpatelh" + - "@maxulysse" diff --git a/modules/pfr/bwa/index/tests/main.nf.test b/modules/pfr/bwa/index/tests/main.nf.test new file mode 100644 index 00000000..2f33c0e8 --- /dev/null +++ b/modules/pfr/bwa/index/tests/main.nf.test @@ -0,0 +1,33 @@ +nextflow_process { + + name "Test Process BWA_INDEX" + tag "modules_nfcore" + tag "modules" + tag "bwa" + tag "bwa/index" + script "../main.nf" + process "BWA_INDEX" + + test("BWA index") { + + when { + process { + """ + input[0] = [ + [id: 'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/pfr/bwa/index/tests/main.nf.test.snap b/modules/pfr/bwa/index/tests/main.nf.test.snap new file mode 100644 index 00000000..e51ad5bf --- /dev/null +++ b/modules/pfr/bwa/index/tests/main.nf.test.snap @@ -0,0 +1,43 @@ +{ + "BWA index": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + [ + "genome.amb:md5,3a68b8b2287e07dd3f5f95f4344ba76e", + "genome.ann:md5,c32e11f6c859f166c7525a9c1d583567", + "genome.bwt:md5,0469c30a1e239dd08f68afe66fde99da", + "genome.pac:md5,983e3d2cd6f36e2546e6d25a0da78d66", + "genome.sa:md5,ab3952cabf026b48cd3eb5bccbb636d1" + ] + ] + ], + "1": [ + "versions.yml:md5,0f20525da90e7489a7ebb02adca3265f" + ], + "index": [ + [ + { + "id": "test" + }, + [ + "genome.amb:md5,3a68b8b2287e07dd3f5f95f4344ba76e", + "genome.ann:md5,c32e11f6c859f166c7525a9c1d583567", + "genome.bwt:md5,0469c30a1e239dd08f68afe66fde99da", + "genome.pac:md5,983e3d2cd6f36e2546e6d25a0da78d66", + "genome.sa:md5,ab3952cabf026b48cd3eb5bccbb636d1" + ] + ] + ], + "versions": [ + "versions.yml:md5,0f20525da90e7489a7ebb02adca3265f" + ] + } + ], + "timestamp": "2023-10-17T17:20:20.180927714" + } +} \ No newline at end of file diff --git a/modules/pfr/bwa/index/tests/tags.yml b/modules/pfr/bwa/index/tests/tags.yml new file mode 100644 index 00000000..28bb483c --- /dev/null +++ b/modules/pfr/bwa/index/tests/tags.yml @@ -0,0 +1,2 @@ +bwa/index: + - modules/nf-core/bwa/index/** diff --git a/modules/pfr/bwa/mem/environment.yml b/modules/pfr/bwa/mem/environment.yml new file mode 100644 index 00000000..3f136d0a --- /dev/null +++ b/modules/pfr/bwa/mem/environment.yml @@ -0,0 +1,10 @@ +name: bwa_mem +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bwa=0.7.17 + # renovate: datasource=conda depName=bioconda/samtools + - samtools=1.19.2 + - htslib=1.19.1 diff --git a/modules/pfr/bwa/mem/main.nf b/modules/pfr/bwa/mem/main.nf new file mode 100644 index 00000000..54ec0f16 --- /dev/null +++ b/modules/pfr/bwa/mem/main.nf @@ -0,0 +1,55 @@ +process BWA_MEM { + tag "$meta.id" + label 'process_high' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:a34558545ae1413d94bde4578787ebef08027945-0' : + 'biocontainers/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:a34558545ae1413d94bde4578787ebef08027945-0' }" + + input: + tuple val(meta), path(reads) + tuple val(meta2), path(index) + val sort_bam + + output: + tuple val(meta), path("*.bam"), emit: bam + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def samtools_command = sort_bam ? 'sort' : 'view' + """ + INDEX=`find -L ./ -name "*.amb" | sed 's/\\.amb\$//'` + + bwa mem \\ + $args \\ + -t $task.cpus \\ + \$INDEX \\ + $reads \\ + | samtools $samtools_command $args2 --threads $task.cpus -o ${prefix}.bam - + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bwa: \$(echo \$(bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//') + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.bam + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bwa: \$(echo \$(bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//') + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ +} diff --git a/modules/pfr/bwa/mem/meta.yml b/modules/pfr/bwa/mem/meta.yml new file mode 100644 index 00000000..440fb1f9 --- /dev/null +++ b/modules/pfr/bwa/mem/meta.yml @@ -0,0 +1,58 @@ +name: bwa_mem +description: Performs fastq alignment to a fasta reference using BWA +keywords: + - mem + - bwa + - alignment + - map + - fastq + - bam + - sam +tools: + - bwa: + description: | + BWA is a software package for mapping DNA sequences against + a large reference genome, such as the human genome. + homepage: http://bio-bwa.sourceforge.net/ + documentation: http://www.htslib.org/doc/samtools.html + arxiv: arXiv:1303.3997 + licence: ["GPL-3.0-or-later"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. + - meta2: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test', single_end:false ] + - index: + type: file + description: BWA genome index files + pattern: "Directory containing BWA index *.{amb,ann,bwt,pac,sa}" + - sort_bam: + type: boolean + description: use samtools sort (true) or samtools view (false) + pattern: "true or false" +output: + - bam: + type: file + description: Output BAM file containing read alignments + pattern: "*.{bam}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" + - "@jeremy1805" +maintainers: + - "@drpatelh" + - "@jeremy1805" diff --git a/modules/pfr/bwa/mem/tests/main.nf.test b/modules/pfr/bwa/mem/tests/main.nf.test new file mode 100644 index 00000000..cd6591ff --- /dev/null +++ b/modules/pfr/bwa/mem/tests/main.nf.test @@ -0,0 +1,173 @@ +nextflow_process { + + name "Test Process BWA_MEM" + tag "modules_nfcore" + tag "modules" + tag "bwa" + tag "bwa/mem" + tag "bwa/index" + script "../main.nf" + process "BWA_MEM" + + test("Single-End") { + + setup { + run("BWA_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = [ + [id: 'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BWA_INDEX.out.index + input[2] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("Single-End Sort") { + + setup { + run("BWA_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = [ + [id: 'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BWA_INDEX.out.index + input[2] = true + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("Paired-End") { + + setup { + run("BWA_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = [ + [id: 'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BWA_INDEX.out.index + input[2] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("Paired-End Sort") { + + setup { + run("BWA_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = [ + [id: 'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BWA_INDEX.out.index + input[2] = true + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } +} diff --git a/modules/pfr/bwa/mem/tests/main.nf.test.snap b/modules/pfr/bwa/mem/tests/main.nf.test.snap new file mode 100644 index 00000000..e4fd8cc0 --- /dev/null +++ b/modules/pfr/bwa/mem/tests/main.nf.test.snap @@ -0,0 +1,142 @@ +{ + "Single-End": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test.bam:md5,a74710a0345b4717bb4431bf9c257120" + ] + ], + "1": [ + "versions.yml:md5,c32f719a68bb2966c8511d808154d42d" + ], + "bam": [ + [ + { + "id": "test", + "single_end": true + }, + "test.bam:md5,a74710a0345b4717bb4431bf9c257120" + ] + ], + "versions": [ + "versions.yml:md5,c32f719a68bb2966c8511d808154d42d" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-19T11:11:48.440661587" + }, + "Single-End Sort": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test.bam:md5,cb1e038bc4d990683fa485d632550b54" + ] + ], + "1": [ + "versions.yml:md5,c32f719a68bb2966c8511d808154d42d" + ], + "bam": [ + [ + { + "id": "test", + "single_end": true + }, + "test.bam:md5,cb1e038bc4d990683fa485d632550b54" + ] + ], + "versions": [ + "versions.yml:md5,c32f719a68bb2966c8511d808154d42d" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-19T11:11:56.086493265" + }, + "Paired-End": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.bam:md5,aea123a3828a99da1906126355f15a12" + ] + ], + "1": [ + "versions.yml:md5,c32f719a68bb2966c8511d808154d42d" + ], + "bam": [ + [ + { + "id": "test", + "single_end": false + }, + "test.bam:md5,aea123a3828a99da1906126355f15a12" + ] + ], + "versions": [ + "versions.yml:md5,c32f719a68bb2966c8511d808154d42d" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-19T11:12:03.474974773" + }, + "Paired-End Sort": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.bam:md5,4682087bcdc3617384b375093fecd8dd" + ] + ], + "1": [ + "versions.yml:md5,c32f719a68bb2966c8511d808154d42d" + ], + "bam": [ + [ + { + "id": "test", + "single_end": false + }, + "test.bam:md5,4682087bcdc3617384b375093fecd8dd" + ] + ], + "versions": [ + "versions.yml:md5,c32f719a68bb2966c8511d808154d42d" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-19T11:12:10.721510817" + } +} \ No newline at end of file diff --git a/modules/pfr/bwa/mem/tests/tags.yml b/modules/pfr/bwa/mem/tests/tags.yml new file mode 100644 index 00000000..82992d1f --- /dev/null +++ b/modules/pfr/bwa/mem/tests/tags.yml @@ -0,0 +1,3 @@ +bwa/mem: + - modules/nf-core/bwa/index/** + - modules/nf-core/bwa/mem/** diff --git a/modules/pfr/samblaster/environment.yml b/modules/pfr/samblaster/environment.yml new file mode 100644 index 00000000..a6e3038a --- /dev/null +++ b/modules/pfr/samblaster/environment.yml @@ -0,0 +1,9 @@ +name: samblaster +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::samblaster=0.1.26 + - bioconda::samtools=1.19.2 + - bioconda::htslib=1.19.1 diff --git a/modules/pfr/samblaster/main.nf b/modules/pfr/samblaster/main.nf new file mode 100644 index 00000000..c9e89af0 --- /dev/null +++ b/modules/pfr/samblaster/main.nf @@ -0,0 +1,50 @@ +process SAMBLASTER { + tag "$meta.id" + label 'process_low' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/mulled-v2-19fa9f1a5c3966b63a24166365e81da35738c5ab:60ebac4ad9c6530c0d7bf6844f52ec6916e1e0b1-0' : + 'biocontainers/mulled-v2-19fa9f1a5c3966b63a24166365e81da35738c5ab:60ebac4ad9c6530c0d7bf6844f52ec6916e1e0b1-0' }" + + input: + tuple val(meta), path(bam) + + output: + tuple val(meta), path("*.bam"), emit: bam + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def args3 = task.ext.args3 ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + if( "$bam" == "${prefix}.bam" ) error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ + samtools view -h $args2 $bam | \\ + samblaster $args | \\ + samtools view $args3 -Sb - >${prefix}.bam + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samblaster: \$( samblaster -h 2>&1 | head -n 1 | sed 's/^samblaster: Version //' ) + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + if( "$bam" == "${prefix}.bam" ) error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ + touch "${prefix}.bam" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samblaster: \$( samblaster -h 2>&1 | head -n 1 | sed 's/^samblaster: Version //' ) + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ +} diff --git a/modules/pfr/samblaster/meta.yml b/modules/pfr/samblaster/meta.yml new file mode 100644 index 00000000..ccb48320 --- /dev/null +++ b/modules/pfr/samblaster/meta.yml @@ -0,0 +1,53 @@ +name: samblaster +description: | + This module combines samtools and samblaster in order to use + samblaster capability to filter or tag SAM files, with the advantage + of maintaining both input and output in BAM format. + Samblaster input must contain a sequence header: for this reason it has been piped + with the "samtools view -h" command. + Additional desired arguments for samtools can be passed using: + options.args2 for the input bam file + options.args3 for the output bam file +keywords: + - sort + - duplicate marking + - bam +tools: + - samblaster: + description: | + samblaster is a fast and flexible program for marking duplicates in read-id grouped paired-end SAM files. + It can also optionally output discordant read pairs and/or split read mappings to separate SAM files, + and/or unmapped/clipped reads to a separate FASTQ file. + By default, samblaster reads SAM input from stdin and writes SAM to stdout. + documentation: https://github.com/GregoryFaust/samblaster + tool_dev_url: https://github.com/GregoryFaust/samblaster + doi: "10.1093/bioinformatics/btu314" + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM file + pattern: "*.bam" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - bam: + type: file + description: Tagged or filtered BAM file + pattern: "*.bam" +authors: + - "@lescai" +maintainers: + - "@lescai" diff --git a/subworkflows/local/fq2hic.nf b/subworkflows/local/fq2hic.nf new file mode 100644 index 00000000..2e26cc21 --- /dev/null +++ b/subworkflows/local/fq2hic.nf @@ -0,0 +1,79 @@ +include { FASTQ_TRIM_FASTP_FASTQC } from '../nf-core/fastq_trim_fastp_fastqc/main' +include { FASTQ_BWA_MEM_SAMBLASTER } from '../pfr/fastq_bwa_mem_samblaster/main' +include { HICQC } from '../../modules/local/hicqc' +include { MAKEAGPFROMFASTA } from '../../modules/local/makeagpfromfasta' +include { AGP2ASSEMBLY } from '../../modules/local/agp2assembly' +include { ASSEMBLY2BEDPE } from '../../modules/local/assembly2bedpe' +include { MATLOCK_BAM2_JUICER } from '../../modules/local/matlock_bam2_juicer' +include { JUICER_SORT } from '../../modules/local/juicer_sort' +include { RUNASSEMBLYVISUALIZER } from '../../modules/local/runassemblyvisualizer' +include { HIC2HTML } from '../../modules/local/hic2html' + +workflow FQ2HIC { + take: + reads // [ val(meta), [ fq ] ] + ref // [ val(meta2), fa ] + hic_skip_fastp // val: true|false + hic_skip_fastqc // val: true|false + + main: + ch_versions = Channel.empty() + + // SUBWORKFLOW: FASTQ_TRIM_FASTP_FASTQC + FASTQ_TRIM_FASTP_FASTQC( + reads, + [], + true, // val_save_trimmed_fail + false, // val_save_merged + hic_skip_fastp, + hic_skip_fastqc + ) + + ch_trim_reads = FASTQ_TRIM_FASTP_FASTQC.out.reads + ch_versions = ch_versions.mix(FASTQ_TRIM_FASTP_FASTQC.out.versions) + + // SUBWORKFLOW: FASTQ_BWA_MEM_SAMBLASTER + FASTQ_BWA_MEM_SAMBLASTER( + ch_trim_reads, + ref.map { meta2, fa -> [ meta2, fa, [] ] } + ) + + ch_bam = FASTQ_BWA_MEM_SAMBLASTER.out.bam + ch_versions = ch_versions.mix(FASTQ_BWA_MEM_SAMBLASTER.out.versions) + + // MODULE: HICQC + ch_bam_and_ref = ch_bam + | map { meta, bam -> [ meta.ref_id, meta, bam ] } + | join( + ref.map { meta2, fa -> [ meta2.id, fa ] } + ) + | map { ref_id, meta, bam, fa -> + [ [ id: "${meta.id}.on.${meta.ref_id}" ], bam, fa ] + } + + HICQC ( ch_bam_and_ref.map { meta3, bam, fa -> [ meta3, bam ] } ) + + ch_versions = ch_versions.mix(HICQC.out.versions) + + // MODULE: MAKEAGPFROMFASTA | AGP2ASSEMBLY | ASSEMBLY2BEDPE + MAKEAGPFROMFASTA ( ch_bam_and_ref.map { meta3, bam, fa -> [ meta3.id, fa ] } ) + | AGP2ASSEMBLY + | ASSEMBLY2BEDPE + + // MODULE: MATLOCK_BAM2_JUICER | JUICER_SORT + MATLOCK_BAM2_JUICER ( ch_bam_and_ref.map { meta3, bam, fa -> [ meta3.id, bam ] } ) + | JUICER_SORT + + // MODULE: RUNASSEMBLYVISUALIZER + RUNASSEMBLYVISUALIZER ( AGP2ASSEMBLY.out.assembly.join(JUICER_SORT.out.links) ) + + ch_hic = RUNASSEMBLYVISUALIZER.out.hic + + // MODULE: HIC2HTML + HIC2HTML ( ch_hic ) + + emit: + hic = ch_hic + html = HIC2HTML.out.html + versions = ch_versions +} diff --git a/subworkflows/pfr/fastq_bwa_mem_samblaster/main.nf b/subworkflows/pfr/fastq_bwa_mem_samblaster/main.nf new file mode 100644 index 00000000..50e824a9 --- /dev/null +++ b/subworkflows/pfr/fastq_bwa_mem_samblaster/main.nf @@ -0,0 +1,61 @@ +include { BWA_INDEX } from '../../../modules/pfr/bwa/index/main' +include { BWA_MEM } from '../../../modules/pfr/bwa/mem/main' +include { SAMBLASTER } from '../../../modules/pfr/samblaster/main' + +workflow FASTQ_BWA_MEM_SAMBLASTER { + + take: + ch_fastq // channel: [ val(meta), [ fq ] ] + ch_reference // channel: [ val(meta2), fasta, index ]; fast | index + + main: + ch_versions = Channel.empty() + + ch_has_index = ch_reference + | branch { meta2, fasta, index -> + yes: index + no: !index + } + + // MODULE: BWA_INDEX + BWA_INDEX ( ch_has_index.no.map { meta2, fasta, index -> [ meta2, fasta ] } ) + + ch_bwa_index = BWA_INDEX.out.index + | mix( + ch_has_index.yes + | map { meta2, fasta, index -> + [ meta2, index ] + } + ) + + ch_versions = ch_versions.mix(BWA_INDEX.out.versions.first()) + + // MODULE: BWA_MEM + ch_mem_inputs = ch_fastq + | combine( + ch_bwa_index + ) + | map { meta, fq, meta2, index -> + [ meta + [ ref_id: meta2.id ], fq, index ] + } + + def sort_bam = false + BWA_MEM( + ch_mem_inputs.map { meta, fq, index -> [ meta, fq ] }, + ch_mem_inputs.map { meta, fq, index -> [ [], index ] }, + sort_bam + ) + + ch_mem_bam = BWA_MEM.out.bam + ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) + + // MODULE: SAMBLASTER + SAMBLASTER ( ch_mem_bam ) + + ch_blasted_bam = SAMBLASTER.out.bam + ch_versions = ch_versions.mix(SAMBLASTER.out.versions.first()) + + emit: + bam = SAMBLASTER.out.bam // channel: [ val(meta), bam ] + versions = ch_versions // channel: [ versions.yml ] +} diff --git a/subworkflows/pfr/fastq_bwa_mem_samblaster/meta.yml b/subworkflows/pfr/fastq_bwa_mem_samblaster/meta.yml new file mode 100644 index 00000000..6eaf302b --- /dev/null +++ b/subworkflows/pfr/fastq_bwa_mem_samblaster/meta.yml @@ -0,0 +1,52 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "fastq_bwa_mem_samblaster" +description: Index fasta if needed, map reads with BWA MEM and filter with samblaster +keywords: + - sort + - bam + - duplicate marking +components: + - bwa/index + - bwa/mem + - samblaster +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. + - meta2: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'genome' ] + - fasta: + type: file + description: Input genome fasta file + - index: + type: file + description: BWA genome index files + pattern: "Directory containing BWA index *.{amb,ann,bwt,pac,sa}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false, ref_id:'genome' ] + - bam: + type: file + description: Tagged or filtered BAM file + pattern: "*.bam" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test b/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test new file mode 100644 index 00000000..1e279e48 --- /dev/null +++ b/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test @@ -0,0 +1,44 @@ +nextflow_workflow { + + name "Test Subworkflow FASTQ_BWA_MEM_SAMBLASTER" + script "../main.nf" + workflow "FASTQ_BWA_MEM_SAMBLASTER" + config './nextflow.config' + + tag "subworkflows" + tag "subworkflows_nfcore" + tag "subworkflows/fastq_bwa_mem_samblaster" + tag "samblaster" + tag "bwa/index" + tag "bwa/mem" + + + test("sarscov2-fq-gz") { + + when { + workflow { + """ + input[0] = Channel.of( + [ + [ id:'test' ], + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + ) + input[1] = Channel.of( + [ [ id: 'genome' ], file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), [] ] + ) + """ + } + } + + then { + assertAll( + { assert workflow.success}, + { assert snapshot(workflow.out).match()} + ) + } + } +} diff --git a/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test.snap b/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test.snap new file mode 100644 index 00000000..e5a9b18e --- /dev/null +++ b/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/main.nf.test.snap @@ -0,0 +1,41 @@ +{ + "sarscov2-fq-gz": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "ref_id": "genome" + }, + "test.on.genome.samblaster.bam:md5,496319fc81c383a9ae6ab52592876c9b" + ] + ], + "1": [ + "versions.yml:md5,2ed54ca5e54063cb579273d0792465a7", + "versions.yml:md5,49d22cda9beaf6ea1a1ad838ef4a4255", + "versions.yml:md5,6a2baa7f2d1d555fe604e451624f414b" + ], + "bam": [ + [ + { + "id": "test", + "ref_id": "genome" + }, + "test.on.genome.samblaster.bam:md5,496319fc81c383a9ae6ab52592876c9b" + ] + ], + "versions": [ + "versions.yml:md5,2ed54ca5e54063cb579273d0792465a7", + "versions.yml:md5,49d22cda9beaf6ea1a1ad838ef4a4255", + "versions.yml:md5,6a2baa7f2d1d555fe604e451624f414b" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-08T19:37:23.464854" + } +} \ No newline at end of file diff --git a/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/nextflow.config b/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/nextflow.config new file mode 100644 index 00000000..23df52b2 --- /dev/null +++ b/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/nextflow.config @@ -0,0 +1,11 @@ +process { + withName: BWA_MEM { + ext.prefix = { "${meta.id}.on.${meta.ref_id}.bwa.mem" } + ext.args = '-5SP' + } + + withName: SAMBLASTER { + ext.prefix = { "${meta.id}.on.${meta.ref_id}.samblaster" } + ext.args3 = '-h -F 2316' + } +} diff --git a/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/tags.yml b/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/tags.yml new file mode 100644 index 00000000..810ecdd8 --- /dev/null +++ b/subworkflows/pfr/fastq_bwa_mem_samblaster/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/fastq_bwa_mem_samblaster: + - subworkflows/pfr/fastq_bwa_mem_samblaster/** diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index 48e3c7d2..9f521a0f 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -35,6 +35,7 @@ include { ASSEMBLATHON_STATS } from '../modules/local/assemblatho include { FASTA_BUSCO_PLOT } from '../subworkflows/local/fasta_busco_plot' include { FASTA_LTRRETRIEVER_LAI } from '../subworkflows/pfr/fasta_ltrretriever_lai/main' include { FASTA_KRAKEN2 } from '../subworkflows/local/fasta_kraken2' +include { FQ2HIC } from '../subworkflows/local/fq2hic' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -50,7 +51,6 @@ include { GUNZIP as GUNZIP_FASTA } from '../modules/nf-core/gunzip/ma include { GUNZIP as GUNZIP_GFF3 } from '../modules/nf-core/gunzip/main' include { FASTAVALIDATOR } from '../modules/nf-core/fastavalidator/main' include { FASTA_EXPLORE_SEARCH_PLOT_TIDK } from '../subworkflows/nf-core/fasta_explore_search_plot_tidk/main' -include { FASTQ_TRIM_FASTP_FASTQC } from '../subworkflows/nf-core/fastq_trim_fastp_fastqc/main' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' @@ -76,7 +76,7 @@ workflow ASSEMBLYQC { } | branch { meta, fasta -> gz: "$fasta".endsWith(".gz") - rest: !"$fasta".endsWith(".gz") + rest: ! "$fasta".endsWith(".gz") } ch_assemby_gff3_branch = ch_input @@ -87,7 +87,7 @@ workflow ASSEMBLYQC { } | branch { meta, gff -> gz: "$gff".endsWith(".gz") - rest: !"$gff".endsWith(".gz") + rest: ! "$gff".endsWith(".gz") } ch_mono_ids = ch_input @@ -97,7 +97,7 @@ workflow ASSEMBLYQC { : null } - ch_hic_reads = !params.hic + ch_hic_reads = ! params.hic ? Channel.empty() : ( "$params.hic".find(/.*[\/].*\.(fastq|fq)\.gz/) @@ -176,7 +176,7 @@ workflow ASSEMBLYQC { | map { tag, report -> def is_clean = file(report).readLines().size < 2 - if (!is_clean) { + if (! is_clean) { log.warn(""" Adaptor contamination detected in ${tag}. See the report for further details. @@ -225,7 +225,7 @@ workflow ASSEMBLYQC { | map { tag, report -> def is_clean = file(report).readLines().size < 3 - if (!is_clean) { + if (! is_clean) { log.warn(""" Foreign organism contamination detected in ${tag}. See the report for further details. @@ -364,19 +364,21 @@ workflow ASSEMBLYQC { ch_kraken2_plot = FASTA_KRAKEN2.out.plot ch_versions = ch_versions.mix(FASTA_KRAKEN2.out.versions) - // SUBWORKFLOW: FASTQ_TRIM_FASTP_FASTQC + // SUBWORKFLOW: FQ2HIC + ch_hic_input_assembly = ! params.hic + ? Channel.empty() + : ch_clean_assembly + | map { tag, fa -> [ [ id: tag ], fa ] } - FASTQ_TRIM_FASTP_FASTQC( + FQ2HIC( ch_hic_reads, - [], - true, // val_save_trimmed_fail - false, // val_save_merged + ch_hic_input_assembly, params.hic_skip_fastp, params.hic_skip_fastqc ) - ch_cleaned_paired_reads = FASTQ_TRIM_FASTP_FASTQC.out.reads - ch_versions = ch_versions.mix(FASTQ_TRIM_FASTP_FASTQC.out.versions) + ch_hic_html = FQ2HIC.out.html + ch_versions = ch_versions.mix(FQ2HIC.out.versions) // MODULE: CUSTOM_DUMPSOFTWAREVERSIONS CUSTOM_DUMPSOFTWAREVERSIONS ( From 1b2632f216ccb2df22cf55add189342916b9e4aa Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 26 Feb 2024 14:56:41 +1300 Subject: [PATCH 28/65] Removed numerous linting issues --- conf/base.config | 4 +++ conf/modules.config | 40 +++++++++++++++++++++ modules/local/agp2assembly.nf | 4 ++- modules/local/assembly2bedpe.nf | 4 ++- modules/local/hic2html.nf | 4 ++- modules/local/hicqc.nf | 4 ++- modules/local/juicer_sort.nf | 3 ++ modules/local/kraken2.nf | 1 - modules/local/makeagpfromfasta.nf | 3 ++ modules/local/matlock_bam2_juicer.nf | 3 ++ modules/local/ncbi_fcs_gx_screen_samples.nf | 1 - modules/local/runassemblyvisualizer.nf | 6 ++-- 12 files changed, 69 insertions(+), 8 deletions(-) diff --git a/conf/base.config b/conf/base.config index 9f5c47e1..895cad8a 100644 --- a/conf/base.config +++ b/conf/base.config @@ -59,8 +59,12 @@ process { maxRetries = 2 } withName:NCBI_FCS_GX_SCREEN_SAMPLES { + time = { check_max( 20.h * task.attempt, 'time' ) } memory = { check_max( 512.GB * task.attempt, 'memory' ) } } + withName:KRAKEN2 { + memory = { check_max( 200.GB * task.attempt, 'memory' ) } + } withName:BWA_MEM { time = { check_max( 2.day * task.attempt, 'time' ) } } diff --git a/conf/modules.config b/conf/modules.config index 60beb771..488c19c0 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -265,4 +265,44 @@ process { ext.prefix = { "${meta.id}.on.${meta.ref_id}.samblaster" } ext.args3 = '-h -F 2316' } + + withName: AGP2ASSEMBLY { + publishDir = [ + path: { "${params.outdir}/hic/assembly" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: ASSEMBLY2BEDPE { + publishDir = [ + path: { "${params.outdir}/hic/bedpe" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: HIC2HTML { + publishDir = [ + path: { "${params.outdir}/hic" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: HICQC { + publishDir = [ + path: { "${params.outdir}/hic/hicqc" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } + + withName: RUNASSEMBLYVISUALIZER { + publishDir = [ + path: { "${params.outdir}/hic" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } } diff --git a/modules/local/agp2assembly.nf b/modules/local/agp2assembly.nf index edb60d96..a658d139 100644 --- a/modules/local/agp2assembly.nf +++ b/modules/local/agp2assembly.nf @@ -3,7 +3,6 @@ process AGP2ASSEMBLY { label 'process_single' container "docker.io/gallvp/juicebox_scripts:a7ae991_ps" - publishDir "${params.outdir}/hic/assembly", mode:'copy' input: tuple val(sample_id_on_tag), path(agp_file) @@ -11,6 +10,9 @@ process AGP2ASSEMBLY { output: tuple val(sample_id_on_tag), path("*.agp.assembly"), emit: assembly + when: + task.ext.when == null || task.ext.when + script: """ assembly_tag=\$(echo $sample_id_on_tag | sed 's/.*\\.on\\.//g') diff --git a/modules/local/assembly2bedpe.nf b/modules/local/assembly2bedpe.nf index d0b7f6ac..b34d80c0 100644 --- a/modules/local/assembly2bedpe.nf +++ b/modules/local/assembly2bedpe.nf @@ -3,7 +3,6 @@ process ASSEMBLY2BEDPE { label 'process_single' container "docker.io/gallvp/python3npkgs:v0.4" - publishDir "${params.outdir}/hic/bedpe", mode:'copy' input: tuple val(sample_id_on_tag), path(agp_assembly_file) @@ -11,6 +10,9 @@ process ASSEMBLY2BEDPE { output: tuple val(sample_id_on_tag), path("*.assembly.bedpe"), emit: bedpe + when: + task.ext.when == null || task.ext.when + script: """ assembly_tag=\$(echo $sample_id_on_tag | sed 's/.*\\.on\\.//g') diff --git a/modules/local/hic2html.nf b/modules/local/hic2html.nf index ba0df637..5a2fc919 100644 --- a/modules/local/hic2html.nf +++ b/modules/local/hic2html.nf @@ -3,7 +3,6 @@ process HIC2HTML { label 'process_single' container "docker.io/gallvp/python3npkgs:v0.4" - publishDir "${params.outdir}/hic", mode: 'copy' input: tuple val(sample_id_on_tag), path(hic_file) @@ -11,6 +10,9 @@ process HIC2HTML { output: path "*.html", emit: html + when: + task.ext.when == null || task.ext.when + script: """ file_name="$hic_file" diff --git a/modules/local/hicqc.nf b/modules/local/hicqc.nf index 0f5c740a..102ff099 100644 --- a/modules/local/hicqc.nf +++ b/modules/local/hicqc.nf @@ -2,7 +2,6 @@ process HICQC { tag "$meta.id" label 'process_single' - publishDir "${params.outdir}/hic/hic_qc", mode:'copy' container "docker.io/gallvp/hic_qc:6881c33_ps" input: @@ -12,6 +11,9 @@ process HICQC { tuple val(meta), path("*.pdf") , emit: pdf path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: """ hic_qc.py \\ diff --git a/modules/local/juicer_sort.nf b/modules/local/juicer_sort.nf index c57124b7..072a6ae7 100644 --- a/modules/local/juicer_sort.nf +++ b/modules/local/juicer_sort.nf @@ -12,6 +12,9 @@ process JUICER_SORT { output: tuple val(sample_id_on_tag), path("*sorted.links.txt"), emit: links + when: + task.ext.when == null || task.ext.when + script: """ sort --parallel=${task.cpus} \\ diff --git a/modules/local/kraken2.nf b/modules/local/kraken2.nf index 27c7e17f..7fa005f4 100644 --- a/modules/local/kraken2.nf +++ b/modules/local/kraken2.nf @@ -1,7 +1,6 @@ process KRAKEN2 { tag "${asm_tag}" label 'process_single' - label 'process_high_memory' container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? 'https://depot.galaxyproject.org/singularity/kraken2:2.1.2--pl5321h9f5acd7_2': diff --git a/modules/local/makeagpfromfasta.nf b/modules/local/makeagpfromfasta.nf index 3c5a0972..81db6e5f 100644 --- a/modules/local/makeagpfromfasta.nf +++ b/modules/local/makeagpfromfasta.nf @@ -10,6 +10,9 @@ process MAKEAGPFROMFASTA { output: tuple val(sample_id_on_tag), path("*.agp"), emit: agp + when: + task.ext.when == null || task.ext.when + script: """ file_name="$assembly_fasta" diff --git a/modules/local/matlock_bam2_juicer.nf b/modules/local/matlock_bam2_juicer.nf index 5222acdf..5d709851 100644 --- a/modules/local/matlock_bam2_juicer.nf +++ b/modules/local/matlock_bam2_juicer.nf @@ -12,6 +12,9 @@ process MATLOCK_BAM2_JUICER { output: tuple val(sample_id_on_tag), path("out.links.txt") + when: + task.ext.when == null || task.ext.when + script: """ matlock bam2 juicer $hic_bam_scaffolds out.links.txt diff --git a/modules/local/ncbi_fcs_gx_screen_samples.nf b/modules/local/ncbi_fcs_gx_screen_samples.nf index 3df915b1..f9ed5cc8 100644 --- a/modules/local/ncbi_fcs_gx_screen_samples.nf +++ b/modules/local/ncbi_fcs_gx_screen_samples.nf @@ -1,7 +1,6 @@ process NCBI_FCS_GX_SCREEN_SAMPLES { tag 'all samples' label 'process_high' - label 'process_long' conda "bioconda::ncbi-fcs-gx=0.5.0" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? diff --git a/modules/local/runassemblyvisualizer.nf b/modules/local/runassemblyvisualizer.nf index a13ed92b..8e8bcd61 100644 --- a/modules/local/runassemblyvisualizer.nf +++ b/modules/local/runassemblyvisualizer.nf @@ -1,8 +1,7 @@ process RUNASSEMBLYVISUALIZER { tag "$sample_id_on_tag" - label "process_medium" + label 'process_medium' - publishDir "${params.outdir}/hic", mode:'copy' container "docker.io/gallvp/3d-dna:63029aa" input: @@ -11,6 +10,9 @@ process RUNASSEMBLYVISUALIZER { output: tuple val(sample_id_on_tag), path("*.hic"), emit: hic + when: + task.ext.when == null || task.ext.when + script: // -p true/false Use GNU Parallel to speed up computation (default is true). """ From 1dd2e26c7fa09f132f8ae7b9d51e22b1c648d399 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 26 Feb 2024 16:44:31 +1300 Subject: [PATCH 29/65] Added versions snippet to multiple modules --- modules/local/agp2assembly.nf | 7 +++++++ modules/local/assembly2bedpe.nf | 15 ++++++++++++++- modules/local/hic2html.nf | 8 +++++++- modules/local/juicer_sort.nf | 8 +++++++- modules/local/makeagpfromfasta.nf | 9 ++++++++- modules/local/matlock_bam2_juicer.nf | 17 ++++++++++++----- modules/local/ncbi_fcs_gx_setup_sample.nf | 8 +++++++- modules/local/runassemblyvisualizer.nf | 13 ++++++++++++- subworkflows/local/fq2hic.nf | 17 ++++++++++++++--- subworkflows/local/ncbi_fcs_gx.nf | 11 +++++++---- 10 files changed, 95 insertions(+), 18 deletions(-) diff --git a/modules/local/agp2assembly.nf b/modules/local/agp2assembly.nf index a658d139..ee615dd6 100644 --- a/modules/local/agp2assembly.nf +++ b/modules/local/agp2assembly.nf @@ -9,13 +9,20 @@ process AGP2ASSEMBLY { output: tuple val(sample_id_on_tag), path("*.agp.assembly"), emit: assembly + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: + def VERSION = '0.1.0' """ assembly_tag=\$(echo $sample_id_on_tag | sed 's/.*\\.on\\.//g') agp2assembly.py $agp_file "\${assembly_tag}.agp.assembly" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + juicebox_scripts: $VERSION + END_VERSIONS """ } diff --git a/modules/local/assembly2bedpe.nf b/modules/local/assembly2bedpe.nf index b34d80c0..ef6cebd7 100644 --- a/modules/local/assembly2bedpe.nf +++ b/modules/local/assembly2bedpe.nf @@ -8,7 +8,8 @@ process ASSEMBLY2BEDPE { tuple val(sample_id_on_tag), path(agp_assembly_file) output: - tuple val(sample_id_on_tag), path("*.assembly.bedpe"), emit: bedpe + tuple val(sample_id_on_tag), path("*.assembly.bedpe") , emit: bedpe + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -17,11 +18,23 @@ process ASSEMBLY2BEDPE { """ assembly_tag=\$(echo $sample_id_on_tag | sed 's/.*\\.on\\.//g') assembly2bedpe.py $agp_assembly_file > "\${assembly_tag}.assembly.bedpe" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + python: \$(python --version | tr -d 'Python[:space:]') + pandas: \$(python -c "import pandas; print(pandas.__version__)") + END_VERSIONS """ stub: """ assembly_tag=\$(echo $sample_id_on_tag | sed 's/.*\\.on\\.//g') touch "\${assembly_tag}.assembly.bedpe" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + python: \$(python --version | tr -d 'Python[:space:]') + pandas: \$(python -c "import pandas; print(pandas.__version__)") + END_VERSIONS """ } diff --git a/modules/local/hic2html.nf b/modules/local/hic2html.nf index 5a2fc919..6149cdfd 100644 --- a/modules/local/hic2html.nf +++ b/modules/local/hic2html.nf @@ -8,7 +8,8 @@ process HIC2HTML { tuple val(sample_id_on_tag), path(hic_file) output: - path "*.html", emit: html + path "*.html" , emit: html + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -17,5 +18,10 @@ process HIC2HTML { """ file_name="$hic_file" hic2html.py "$hic_file" > "\${file_name%.*}.html" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + python: \$(python --version | tr -d 'Python[:space:]') + END_VERSIONS """ } diff --git a/modules/local/juicer_sort.nf b/modules/local/juicer_sort.nf index 072a6ae7..4b0645bd 100644 --- a/modules/local/juicer_sort.nf +++ b/modules/local/juicer_sort.nf @@ -10,7 +10,8 @@ process JUICER_SORT { tuple val(sample_id_on_tag), path(out_links_txt) output: - tuple val(sample_id_on_tag), path("*sorted.links.txt"), emit: links + tuple val(sample_id_on_tag), path("*sorted.links.txt") , emit: links + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -21,5 +22,10 @@ process JUICER_SORT { -k2,2 -k6,6 \\ $out_links_txt \\ > out.sorted.links.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + sort: \$(sort --version | sed -n '/sort (GNU coreutils) / s/sort (GNU coreutils) //p') + END_VERSIONS """ } diff --git a/modules/local/makeagpfromfasta.nf b/modules/local/makeagpfromfasta.nf index 81db6e5f..3b6a4e79 100644 --- a/modules/local/makeagpfromfasta.nf +++ b/modules/local/makeagpfromfasta.nf @@ -8,14 +8,21 @@ process MAKEAGPFROMFASTA { tuple val(sample_id_on_tag), path(assembly_fasta) output: - tuple val(sample_id_on_tag), path("*.agp"), emit: agp + tuple val(sample_id_on_tag), path("*.agp") , emit: agp + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: + def VERSION = '0.1.0' """ file_name="$assembly_fasta" makeAgpFromFasta.py $assembly_fasta "\${file_name%%.*}.agp" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + juicebox_scripts: $VERSION + END_VERSIONS """ } diff --git a/modules/local/matlock_bam2_juicer.nf b/modules/local/matlock_bam2_juicer.nf index 5d709851..dadb03d2 100644 --- a/modules/local/matlock_bam2_juicer.nf +++ b/modules/local/matlock_bam2_juicer.nf @@ -7,16 +7,23 @@ process MATLOCK_BAM2_JUICER { 'biocontainers/matlock:20181227--h4b03ef3_3' }" input: - tuple val(sample_id_on_tag), path(hic_bam_scaffolds) + tuple val(sample_id_on_tag), path(hic_bam_scaffolds) output: - tuple val(sample_id_on_tag), path("out.links.txt") + tuple val(sample_id_on_tag), path("out.links.txt") , emit: links + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: - """ - matlock bam2 juicer $hic_bam_scaffolds out.links.txt - """ + def VERSION = '20181227' + """ + matlock bam2 juicer $hic_bam_scaffolds out.links.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + matlock: $VERSION + END_VERSIONS + """ } diff --git a/modules/local/ncbi_fcs_gx_setup_sample.nf b/modules/local/ncbi_fcs_gx_setup_sample.nf index a5f72385..8b36ad8f 100644 --- a/modules/local/ncbi_fcs_gx_setup_sample.nf +++ b/modules/local/ncbi_fcs_gx_setup_sample.nf @@ -10,7 +10,8 @@ process NCBI_FCS_GX_SETUP_SAMPLE { tuple val(asm_tag), path(fasta_file) output: - path 'fasta.file.for.*.fasta', emit: fsata + path 'fasta.file.for.*.fasta' , emit: fsata + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -22,5 +23,10 @@ process NCBI_FCS_GX_SETUP_SAMPLE { } """ ln -s $fasta_file "fasta.file.for.${asm_tag}.fasta" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + ubuntu: \$(cat /etc/issue | tr -d 'Ubuntu LTS[:space:]\\\\') + END_VERSIONS """ } diff --git a/modules/local/runassemblyvisualizer.nf b/modules/local/runassemblyvisualizer.nf index 8e8bcd61..7b628d08 100644 --- a/modules/local/runassemblyvisualizer.nf +++ b/modules/local/runassemblyvisualizer.nf @@ -8,7 +8,8 @@ process RUNASSEMBLYVISUALIZER { tuple val(sample_id_on_tag), path(agp_assembly_file), path(sorted_links_txt_file) output: - tuple val(sample_id_on_tag), path("*.hic"), emit: hic + tuple val(sample_id_on_tag), path("*.hic") , emit: hic + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -23,11 +24,21 @@ process RUNASSEMBLYVISUALIZER { $agp_assembly_file $sorted_links_txt_file mv "\${file_name%.*}.hic" "\${assembly_tag}.hic" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + run-assembly-visualizer.sh: \$(/usr/src/3d-dna/visualize/run-assembly-visualizer.sh -h | sed -n '/Visualizing draft genomes in juicebox:/ s/Visualizing draft genomes in juicebox: //p') + END_VERSIONS """ stub: """ assembly_tag=\$(echo $sample_id_on_tag | sed 's/.*\\.on\\.//g') touch "\${assembly_tag}.hic" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + run-assembly-visualizer.sh: \$(/usr/src/3d-dna/visualize/run-assembly-visualizer.sh -h | sed -n '/Visualizing draft genomes in juicebox:/ s/Visualizing draft genomes in juicebox: //p') + END_VERSIONS """ } diff --git a/subworkflows/local/fq2hic.nf b/subworkflows/local/fq2hic.nf index 2e26cc21..f8a3eaa9 100644 --- a/subworkflows/local/fq2hic.nf +++ b/subworkflows/local/fq2hic.nf @@ -57,21 +57,32 @@ workflow FQ2HIC { // MODULE: MAKEAGPFROMFASTA | AGP2ASSEMBLY | ASSEMBLY2BEDPE MAKEAGPFROMFASTA ( ch_bam_and_ref.map { meta3, bam, fa -> [ meta3.id, fa ] } ) - | AGP2ASSEMBLY - | ASSEMBLY2BEDPE + AGP2ASSEMBLY ( MAKEAGPFROMFASTA.out.agp ) + ASSEMBLY2BEDPE ( AGP2ASSEMBLY.out.assembly ) + + ch_versions = ch_versions.mix(MAKEAGPFROMFASTA.out.versions.first()) + | mix(AGP2ASSEMBLY.out.versions.first()) + | mix(ASSEMBLY2BEDPE.out.versions.first()) // MODULE: MATLOCK_BAM2_JUICER | JUICER_SORT MATLOCK_BAM2_JUICER ( ch_bam_and_ref.map { meta3, bam, fa -> [ meta3.id, bam ] } ) - | JUICER_SORT + + JUICER_SORT ( MATLOCK_BAM2_JUICER.out.links ) + + ch_versions = ch_versions.mix(MATLOCK_BAM2_JUICER.out.versions.first()) + | mix(JUICER_SORT.out.versions.first()) // MODULE: RUNASSEMBLYVISUALIZER RUNASSEMBLYVISUALIZER ( AGP2ASSEMBLY.out.assembly.join(JUICER_SORT.out.links) ) ch_hic = RUNASSEMBLYVISUALIZER.out.hic + ch_versions = ch_versions.mix(RUNASSEMBLYVISUALIZER.out.versions.first()) // MODULE: HIC2HTML HIC2HTML ( ch_hic ) + ch_versions = ch_versions.mix(HIC2HTML.out.versions.first()) + emit: hic = ch_hic html = HIC2HTML.out.html diff --git a/subworkflows/local/ncbi_fcs_gx.nf b/subworkflows/local/ncbi_fcs_gx.nf index bcc9350d..0425a916 100644 --- a/subworkflows/local/ncbi_fcs_gx.nf +++ b/subworkflows/local/ncbi_fcs_gx.nf @@ -9,12 +9,16 @@ workflow NCBI_FCS_GX { tax_id // val: Integer main: + ch_versions = Channel.empty() + // MODULE: NCBI_FCS_GX_SETUP_SAMPLE NCBI_FCS_GX_SETUP_SAMPLE ( tuple_of_tag_file ) ch_all_samples = NCBI_FCS_GX_SETUP_SAMPLE.out.fsata | collect + ch_versions = ch_versions.mix(NCBI_FCS_GX_SETUP_SAMPLE.out.versions.first()) + // MODULE: NCBI_FCS_GX_SCREEN_SAMPLES NCBI_FCS_GX_SCREEN_SAMPLES( ch_all_samples, @@ -38,14 +42,13 @@ workflow NCBI_FCS_GX { [tag, it] } + ch_versions = ch_versions.mix(NCBI_FCS_GX_SCREEN_SAMPLES.out.versions) + // MODULE: NCBI_FCS_GX_KRONA_PLOT NCBI_FCS_GX_KRONA_PLOT ( ch_gx_taxonomy ) ch_gx_taxonomy_plot = NCBI_FCS_GX_KRONA_PLOT.out.plot - - ch_versions = Channel.empty() - | mix(NCBI_FCS_GX_SCREEN_SAMPLES.out.versions.first()) - | mix(NCBI_FCS_GX_KRONA_PLOT.out.versions.first()) + ch_versions = ch_versions.mix(NCBI_FCS_GX_KRONA_PLOT.out.versions.first()) emit: gx_report = ch_gx_report From fa0b29404653cf65dee373c09910fb171ce2c1fd Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 26 Feb 2024 16:59:10 +1300 Subject: [PATCH 30/65] Fixed path in stub assemblysheet --- tests/stub/assemblysheet.csv | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/tests/stub/assemblysheet.csv b/tests/stub/assemblysheet.csv index 69b0eb4f..06b8a783 100644 --- a/tests/stub/assemblysheet.csv +++ b/tests/stub/assemblysheet.csv @@ -1,2 +1,2 @@ tag,fasta,gff3,monoploid_ids,synteny_labels -FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz,https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/tests/stub/FI1.monoploid.seqs.txt,https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/FI1.seq.labels.tsv +FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz,https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/tests/stub/FI1.monoploid.seqs.txt,https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/tests/stub/FI1.seq.labels.tsv From fdd04ecbb3bb6eb5f7c5f8426c8478ccc4174948 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 27 Feb 2024 14:03:23 +1300 Subject: [PATCH 31/65] Added fasta_synteny sub workflow --- assets/schema_xref_assemblies.json | 32 +++ bin/colorbundlesbycontig.py | 77 ++++++ bin/colorbundlesbysize.pl | 181 +++++++++++++ bin/validateseqlists.sh | 39 +++ conf/base.config | 3 + modules/local/circos.nf | 151 +++++++++++ modules/local/circos_bundlelinks.nf | 39 +++ modules/local/colourbundlelinks.nf | 48 ++++ modules/local/dnadiff.nf | 53 ++++ modules/local/filtersortfasta.nf | 53 ++++ modules/local/generatekaryotype.nf | 59 ++++ modules/local/getfastalength.nf | 55 ++++ modules/local/mummer.nf | 41 +++ modules/local/relabelbundlelinks.nf | 51 ++++ modules/local/relabelfastalength.nf | 54 ++++ modules/local/splitbundlefile.nf | 42 +++ nextflow.config | 4 +- nextflow_schema.json | 16 +- subworkflows/local/fasta_synteny.nf | 253 ++++++++++++++++++ ...2021a.seq.list => TT_2021a.seq.labels.tsv} | 0 tests/stub/stub.config | 9 +- tests/stub/xrefsheet.csv | 2 + workflows/assemblyqc.nf | 37 +++ 23 files changed, 1286 insertions(+), 13 deletions(-) create mode 100644 assets/schema_xref_assemblies.json create mode 100755 bin/colorbundlesbycontig.py create mode 100755 bin/colorbundlesbysize.pl create mode 100755 bin/validateseqlists.sh create mode 100644 modules/local/circos.nf create mode 100644 modules/local/circos_bundlelinks.nf create mode 100644 modules/local/colourbundlelinks.nf create mode 100644 modules/local/dnadiff.nf create mode 100644 modules/local/filtersortfasta.nf create mode 100644 modules/local/generatekaryotype.nf create mode 100644 modules/local/getfastalength.nf create mode 100644 modules/local/mummer.nf create mode 100644 modules/local/relabelbundlelinks.nf create mode 100644 modules/local/relabelfastalength.nf create mode 100644 modules/local/splitbundlefile.nf create mode 100644 subworkflows/local/fasta_synteny.nf rename tests/stub/{TT_2021a.seq.list => TT_2021a.seq.labels.tsv} (100%) create mode 100644 tests/stub/xrefsheet.csv diff --git a/assets/schema_xref_assemblies.json b/assets/schema_xref_assemblies.json new file mode 100644 index 00000000..e4a5a40a --- /dev/null +++ b/assets/schema_xref_assemblies.json @@ -0,0 +1,32 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/master/assets/schema_input.json", + "title": "plant-food-research-open/assemblyqc pipeline - params.synteny_xref_assemblies schema", + "description": "Schema for the file provided with params.synteny_xref_assemblies", + "type": "array", + "items": { + "type": "object", + "properties": { + "tag": { + "type": "string", + "pattern": "^\\w+$", + "errorMessage": "Assembly tags must be provided and can only contain alphanumeric characters including '_'" + }, + "fasta": { + "type": "string", + "pattern": "^\\S+\\.f(ast|as|sa|na)?\\.gz$", + "errorMessage": "FASTA file path cannot contain spaces and must have extension '.f(ast|as|sa|na)' or '.f(ast|as|sa|na).gz'" + }, + "synteny_labels": { + "errorMessage": "Synteny labels tsv path cannot contain spaces and must have extension '.tsv'", + "anyOf": [ + { + "type": "string", + "pattern": "^\\S+\\.tsv$" + } + ] + } + }, + "required": ["tag", "fasta", "synteny_labels"] + } +} diff --git a/bin/colorbundlesbycontig.py b/bin/colorbundlesbycontig.py new file mode 100755 index 00000000..d501323c --- /dev/null +++ b/bin/colorbundlesbycontig.py @@ -0,0 +1,77 @@ +#!/usr/bin/env python + +import sys +import re + +bundled_links_file_name = sys.argv[1] + + +def natural_key(string): + """Return a list of keys that sort naturally.""" + return [int(s) if s.isdigit() else s for s in re.split(r"(\d+)", string)] + + +def hsv2rgb(h, s, v): + """Convert HSV color to RGB color.""" + h = float(h) + s = float(s) + v = float(v) + h60 = h / 60.0 + h60f = int(h60) + hi = int(h60f) % 6 + f = h60 - h60f + p = v * (1 - s) + q = v * (1 - f * s) + t = v * (1 - (1 - f) * s) + r, g, b = 0, 0, 0 + if hi == 0: + r, g, b = v, t, p + elif hi == 1: + r, g, b = q, v, p + elif hi == 2: + r, g, b = p, v, t + elif hi == 3: + r, g, b = p, q, v + elif hi == 4: + r, g, b = t, p, v + elif hi == 5: + r, g, b = v, p, q + return int(r * 255), int(g * 255), int(b * 255) + + +def generate_colors(num_colors): + """Generate a list of colors""" + hue_step = int(360 / num_colors) + hue = 0 + colors = [] + for i in range(num_colors): + red, green, blue = hsv2rgb(hue, 0.8, 0.8) + colors.append(f"{red},{green},{blue},0.5") + hue += hue_step + return colors + + +def read_file_lines(file_path): + with open(file_path, "r") as f: + return f.readlines() + + +def generate_colors_by_ids(bundle_file_lines): + """Create a dictionary to map unique target ids to colors""" + unique_ids = set( + line.split()[3] for line in bundle_file_lines + ) # index 3: Target ids + num_unique_ids = len(unique_ids) + colors = generate_colors(num_unique_ids) + return dict(zip(sorted(unique_ids, key=natural_key), colors)) + + +if __name__ == "__main__": + bundle_file_lines = read_file_lines(bundled_links_file_name) + id_to_color = generate_colors_by_ids(bundle_file_lines) + + for line in bundle_file_lines: + parts = line.strip().split() + unique_id = parts[3] # index 3: Target ids + color = id_to_color[unique_id] + print(" ".join(parts[0:6] + [f"color=({color})", parts[6]])) diff --git a/bin/colorbundlesbysize.pl b/bin/colorbundlesbysize.pl new file mode 100755 index 00000000..8050e3c0 --- /dev/null +++ b/bin/colorbundlesbysize.pl @@ -0,0 +1,181 @@ +#!/usr/bin/perl +use strict; +use warnings; + +=head1 DESCRIPTION + +Adds colours to a CIRCOS bundle file. + +=head1 AUTHOR + +Original: Ross Crowhurst L +Modified: Usman Rashid L + +=cut + +my $low = 0; + +my %bundleColorsRGB = ( + 3000 => "128,0,0,0.5", + 2000 => "229,0,10,0.5", + 1500 => "229,19,9,0.5", + 1000 => "216,38,8,0.5", + 500 => "210,57,7,0.5", + 250 => "204,76,6,0.5", + 100 => "198,95,5,0.5", + 50 => "192,114,4,0.5", + 25 => "186,113,3,0.5", + 10 => "180,152,2,0.5", + 5 => "174,171,1,0.5", + 0 => "168,191,0,0.5" +); + +my %bundleColorsRGBLow = ( + 55 => "128,0,0,0.5", + 50 => "229,0,10,0.5", + 45 => "229,19,9,0.5", + 40 => "216,38,8,0.5", + 35 => "210,57,7,0.5", + 30 => "204,76,6,0.5", + 25 => "198,95,5,0.5", + 20 => "192,114,4,0.5", + 15 => "186,113,3,0.5", + 10 => "180,152,2,0.5", + 5 => "174,171,1,0.5", + 0 => "168,191,0,0.5" +); + +sub usage { + print "USAGE: $0 -i=bundle_file_in -o=colored_bundle_file_out [-low]\n"; + print "To get colors:\n\n"; + print " $0 -colorsRGB [or -colorsRGBAsHTMLTable] [-low]\n"; + print "or\n"; + print " $0 -colorsHex [-low]\n"; + print "or\n"; + print " $0 -colorsHexAsHTMLKeyTable [-low]\n"; + exit(0); +} + +sub exportRGB { + if ($low) + { + foreach my $threshold (sort {$a <=> $b} keys %bundleColorsRGBLow) + { + print "$threshold\t$bundleColorsRGBLow{$threshold}\n"; + } + } + else + { + foreach my $threshold (sort {$a <=> $b} keys %bundleColorsRGB) + { + print "$threshold\t$bundleColorsRGB{$threshold}\n"; + } + } + exit(0); +} + +sub exportRGBHTMLTable { + print "
    Process Name \\", + " \\ Software Version
    CUSTOM_DUMPSOFTWAREVERSIONSpython3.11.7
    yaml5.4.1
    TOOL1tool10.11.9
    TOOL2tool21.9
    WorkflowNextflow
    File typeConventional base calls
    12.922000 K (92.984097%)", + "single end (151 cycles)" ] + def log_text = [ "Q20 bases: 12922(92.9841%)", + "reads passed filter: 99" ] + def read_lines = ["@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1", + "TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT", + "AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE + { assert path(process.out.reads.get(0).get(1)).linesGzip.contains(read_line) } + } + }, + { html_text.each { html_part -> + { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } + } + }, + { assert snapshot(process.out.json).match("test_fastp_single_end_json") }, + { log_text.each { log_part -> + { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } + } + }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { file(it[1]).getName() } + + process.out.json.collect { file(it[1]).getName() } + + process.out.html.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.reads_fail.collect { file(it[1]).getName() } + + process.out.reads_merged.collect { file(it[1]).getName() } + ).sort() + ).match("test_fastp_single_end-_match") + }, + { assert snapshot(process.out.versions).match("versions_single_end") } + ) + } + } + + test("test_fastp_single_end-stub") { + + options '-stub' + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = false + save_merged = false + + input[0] = Channel.of([ + [ id:'test', single_end:true ], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + + assertAll( + { assert process.success }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { file(it[1]).getName() } + + process.out.json.collect { file(it[1]).getName() } + + process.out.html.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.reads_fail.collect { file(it[1]).getName() } + + process.out.reads_merged.collect { file(it[1]).getName() } + ).sort() + ).match("test_fastp_single_end-for_stub_match") + }, + { assert snapshot(process.out.versions).match("versions_single_end_stub") } + ) + } + } + + test("test_fastp_paired_end") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = false + save_merged = false + + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + def html_text = [ "Q20 bases:25.719000 K (93.033098%)", + "The input has little adapter percentage (~0.000000%), probably it's trimmed before."] + def log_text = [ "No adapter detected for read1", + "Q30 bases: 12281(88.3716%)"] + def json_text = ['"passed_filter_reads": 198'] + def read1_lines = ["@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1", + "TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT", + "AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE + { assert path(process.out.reads.get(0).get(1).get(0)).linesGzip.contains(read1_line) } + } + }, + { read2_lines.each { read2_line -> + { assert path(process.out.reads.get(0).get(1).get(1)).linesGzip.contains(read2_line) } + } + }, + { html_text.each { html_part -> + { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } + } + }, + { json_text.each { json_part -> + { assert path(process.out.json.get(0).get(1)).getText().contains(json_part) } + } + }, + { log_text.each { log_part -> + { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } + } + }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { it[1].collect { item -> file(item).getName() } } + + process.out.json.collect { file(it[1]).getName() } + + process.out.html.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.reads_fail.collect { file(it[1]).getName() } + + process.out.reads_merged.collect { file(it[1]).getName() } + ).sort() + ).match("test_fastp_paired_end_match") + }, + { assert snapshot(process.out.versions).match("versions_paired_end") } + ) + } + } + + test("test_fastp_paired_end-stub") { + + options '-stub' + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = false + save_merged = false + + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + assertAll( + { assert process.success }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { it[1].collect { item -> file(item).getName() } } + + process.out.json.collect { file(it[1]).getName() } + + process.out.html.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.reads_fail.collect { file(it[1]).getName() } + + process.out.reads_merged.collect { file(it[1]).getName() } + ).sort() + ).match("test_fastp_paired_end-for_stub_match") + }, + { assert snapshot(process.out.versions).match("versions_paired_end-stub") } + ) + } + } + + test("fastp test_fastp_interleaved") { + config './nextflow.config' + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = false + save_merged = false + + input[0] = Channel.of([ + [ id:'test', single_end:true ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) ] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + def html_text = [ "Q20 bases:25.719000 K (93.033098%)", + "paired end (151 cycles + 151 cycles)"] + def log_text = [ "Q20 bases: 12922(92.9841%)", + "reads passed filter: 198"] + def read_lines = [ "@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1", + "TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT", + "AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE + { assert path(process.out.reads.get(0).get(1)).linesGzip.contains(read_line) } + } + }, + { html_text.each { html_part -> + { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } + } + }, + { assert snapshot(process.out.json).match("fastp test_fastp_interleaved_json") }, + { log_text.each { log_part -> + { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } + } + }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { file(it[1]).getName() } + + process.out.json.collect { file(it[1]).getName() } + + process.out.html.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.reads_fail.collect { file(it[1]).getName() } + + process.out.reads_merged.collect { file(it[1]).getName() } + ).sort() + ).match("test_fastp_interleaved-_match") + }, + { assert snapshot(process.out.versions).match("versions_interleaved") } + ) + } + } + + test("fastp test_fastp_interleaved-stub") { + + options '-stub' + + config './nextflow.config' + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = false + save_merged = false + + input[0] = Channel.of([ + [ id:'test', single_end:true ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) ] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + assertAll( + { assert process.success }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { file(it[1]).getName() } + + process.out.json.collect { file(it[1]).getName() } + + process.out.html.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.reads_fail.collect { file(it[1]).getName() } + + process.out.reads_merged.collect { file(it[1]).getName() } + ).sort() + ).match("test_fastp_interleaved-for_stub_match") + }, + { assert snapshot(process.out.versions).match("versions_interleaved-stub") } + ) + } + } + + test("test_fastp_single_end_trim_fail") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = true + save_merged = false + + input[0] = Channel.of([ + [ id:'test', single_end:true ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + def html_text = [ "Q20 bases:12.922000 K (92.984097%)", + "single end (151 cycles)"] + def log_text = [ "Q20 bases: 12922(92.9841%)", + "reads passed filter: 99" ] + def read_lines = [ "@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1", + "TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT", + "AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE + { assert path(process.out.reads.get(0).get(1)).linesGzip.contains(read_line) } + } + }, + { failed_read_lines.each { failed_read_line -> + { assert path(process.out.reads_fail.get(0).get(1)).linesGzip.contains(failed_read_line) } + } + }, + { html_text.each { html_part -> + { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } + } + }, + { assert snapshot(process.out.json).match("test_fastp_single_end_trim_fail_json") }, + { log_text.each { log_part -> + { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } + } + }, + { assert snapshot(process.out.versions).match("versions_single_end_trim_fail") } + ) + } + } + + test("test_fastp_paired_end_trim_fail") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = true + save_merged = false + + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + def html_text = [ "Q20 bases:25.719000 K (93.033098%)", + "The input has little adapter percentage (~0.000000%), probably it's trimmed before."] + def log_text = [ "No adapter detected for read1", + "Q30 bases: 12281(88.3716%)"] + def json_text = ['"passed_filter_reads": 198'] + def read1_lines = ["@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1", + "TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT", + "AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE + { assert path(process.out.reads.get(0).get(1).get(0)).linesGzip.contains(read1_line) } + } + }, + { read2_lines.each { read2_line -> + { assert path(process.out.reads.get(0).get(1).get(1)).linesGzip.contains(read2_line) } + } + }, + { failed_read2_lines.each { failed_read2_line -> + { assert path(process.out.reads_fail.get(0).get(1).get(1)).linesGzip.contains(failed_read2_line) } + } + }, + { html_text.each { html_part -> + { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } + } + }, + { json_text.each { json_part -> + { assert path(process.out.json.get(0).get(1)).getText().contains(json_part) } + } + }, + { log_text.each { log_part -> + { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } + } + }, + { assert snapshot(process.out.versions).match("versions_paired_end_trim_fail") } + ) + } + } + + test("test_fastp_paired_end_merged") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = false + save_merged = true + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + def html_text = [ "
    "] + def log_text = [ "Merged and filtered:", + "total reads: 75", + "total bases: 13683"] + def json_text = ['"merged_and_filtered": {', '"total_reads": 75', '"total_bases": 13683'] + def read1_lines = [ "@ERR5069949.1066259 NS500628:121:HK3MMAFX2:1:11312:18369:8333/1", + "CCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTC", + "AAAAAEAEEAEEEEEEEEEEEEEEEEAEEEEAEEEEEEEEAEEEEEEEEEEEEEEEEE/EAEEEEEE/6EEEEEEEEEEAEEAEEE/EE/AEEAEEEEEAEEEA/EEAAEAE + { assert path(process.out.reads.get(0).get(1).get(0)).linesGzip.contains(read1_line) } + } + }, + { read2_lines.each { read2_line -> + { assert path(process.out.reads.get(0).get(1).get(1)).linesGzip.contains(read2_line) } + } + }, + { read_merged_lines.each { read_merged_line -> + { assert path(process.out.reads_merged.get(0).get(1)).linesGzip.contains(read_merged_line) } + } + }, + { html_text.each { html_part -> + { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } + } + }, + { json_text.each { json_part -> + { assert path(process.out.json.get(0).get(1)).getText().contains(json_part) } + } + }, + { log_text.each { log_part -> + { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } + } + }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { it[1].collect { item -> file(item).getName() } } + + process.out.json.collect { file(it[1]).getName() } + + process.out.html.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.reads_fail.collect { file(it[1]).getName() } + + process.out.reads_merged.collect { file(it[1]).getName() } + ).sort() + ).match("test_fastp_paired_end_merged_match") + }, + { assert snapshot(process.out.versions).match("versions_paired_end_merged") } + ) + } + } + + test("test_fastp_paired_end_merged-stub") { + + options '-stub' + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = [] + save_trimmed_fail = false + save_merged = true + + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + assertAll( + { assert process.success }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { it[1].collect { item -> file(item).getName() } } + + process.out.json.collect { file(it[1]).getName() } + + process.out.html.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.reads_fail.collect { file(it[1]).getName() } + + process.out.reads_merged.collect { file(it[1]).getName() } + ).sort() + ).match("test_fastp_paired_end_merged-for_stub_match") + }, + { assert snapshot(process.out.versions).match("versions_paired_end_merged_stub") } + ) + } + } + + test("test_fastp_paired_end_merged_adapterlist") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + adapter_fasta = Channel.of([ file(params.modules_testdata_base_path + 'delete_me/fastp/adapters.fasta', checkIfExists: true) ]) + save_trimmed_fail = false + save_merged = true + + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + input[1] = adapter_fasta + input[2] = save_trimmed_fail + input[3] = save_merged + """ + } + } + + then { + def html_text = [ "
    "] + def log_text = [ "Merged and filtered:", + "total reads: 75", + "total bases: 13683"] + def json_text = ['"merged_and_filtered": {', '"total_reads": 75', '"total_bases": 13683',"--adapter_fasta"] + def read1_lines = ["@ERR5069949.1066259 NS500628:121:HK3MMAFX2:1:11312:18369:8333/1", + "CCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTC", + "AAAAAEAEEAEEEEEEEEEEEEEEEEAEEEEAEEEEEEEEAEEEEEEEEEEEEEEEEE/EAEEEEEE/6EEEEEEEEEEAEEAEEE/EE/AEEAEEEEEAEEEA/EEAAEAE + { assert path(process.out.reads.get(0).get(1).get(0)).linesGzip.contains(read1_line) } + } + }, + { read2_lines.each { read2_line -> + { assert path(process.out.reads.get(0).get(1).get(1)).linesGzip.contains(read2_line) } + } + }, + { read_merged_lines.each { read_merged_line -> + { assert path(process.out.reads_merged.get(0).get(1)).linesGzip.contains(read_merged_line) } + } + }, + { html_text.each { html_part -> + { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) } + } + }, + { json_text.each { json_part -> + { assert path(process.out.json.get(0).get(1)).getText().contains(json_part) } + } + }, + { log_text.each { log_part -> + { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } + } + }, + { assert snapshot(process.out.versions).match("versions_paired_end_merged_adapterlist") } + ) + } + } +} diff --git a/modules/nf-core/fastp/tests/main.nf.test.snap b/modules/nf-core/fastp/tests/main.nf.test.snap new file mode 100644 index 00000000..b4c0e1dd --- /dev/null +++ b/modules/nf-core/fastp/tests/main.nf.test.snap @@ -0,0 +1,330 @@ +{ + "fastp test_fastp_interleaved_json": { + "content": [ + [ + [ + { + "id": "test", + "single_end": true + }, + "test.fastp.json:md5,168f516f7bd4b7b6c32da7cba87299a4" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-17T18:08:06.123035" + }, + "test_fastp_paired_end_merged-for_stub_match": { + "content": [ + [ + [ + "test_1.fastp.fastq.gz", + "test_2.fastp.fastq.gz" + ], + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "test.merged.fastq.gz", + "{id=test, single_end=false}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-17T18:10:13.467574" + }, + "versions_interleaved": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:56:24.615634793" + }, + "test_fastp_single_end_json": { + "content": [ + [ + [ + { + "id": "test", + "single_end": true + }, + "test.fastp.json:md5,c852d7a6dba5819e4ac8d9673bedcacc" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-17T18:06:00.223817" + }, + "versions_paired_end": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:55:42.333545689" + }, + "test_fastp_paired_end_match": { + "content": [ + [ + [ + "test_1.fastp.fastq.gz", + "test_2.fastp.fastq.gz" + ], + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "{id=test, single_end=false}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T12:03:06.431833729" + }, + "test_fastp_interleaved-_match": { + "content": [ + [ + "test.fastp.fastq.gz", + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "{id=test, single_end=true}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T12:03:37.827323085" + }, + "test_fastp_paired_end_merged_match": { + "content": [ + [ + [ + "test_1.fastp.fastq.gz", + "test_2.fastp.fastq.gz" + ], + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "test.merged.fastq.gz", + "{id=test, single_end=false}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T12:08:44.496251446" + }, + "versions_single_end_stub": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:55:27.354051299" + }, + "versions_interleaved-stub": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:56:46.535528418" + }, + "versions_single_end_trim_fail": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:59:03.724591407" + }, + "test_fastp_paired_end-for_stub_match": { + "content": [ + [ + [ + "test_1.fastp.fastq.gz", + "test_2.fastp.fastq.gz" + ], + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "{id=test, single_end=false}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-17T18:07:15.398827" + }, + "versions_paired_end-stub": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:56:06.50017282" + }, + "versions_single_end": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:55:07.67921647" + }, + "versions_paired_end_merged_stub": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:59:47.350653154" + }, + "test_fastp_interleaved-for_stub_match": { + "content": [ + [ + "test.fastp.fastq.gz", + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "{id=test, single_end=true}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-17T18:08:06.127974" + }, + "versions_paired_end_trim_fail": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:59:18.140484878" + }, + "test_fastp_single_end-for_stub_match": { + "content": [ + [ + "test.fastp.fastq.gz", + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "{id=test, single_end=true}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-17T18:06:00.244202" + }, + "test_fastp_single_end-_match": { + "content": [ + [ + "test.fastp.fastq.gz", + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "{id=test, single_end=true}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:57:30.791982648" + }, + "versions_paired_end_merged_adapterlist": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T12:05:37.845370554" + }, + "versions_paired_end_merged": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:59:32.860543858" + }, + "test_fastp_single_end_trim_fail_json": { + "content": [ + [ + [ + { + "id": "test", + "single_end": true + }, + "test.fastp.json:md5,9a7ee180f000e8d00c7fb67f06293eb5" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-17T18:08:41.942317" + } +} \ No newline at end of file diff --git a/modules/nf-core/fastp/tests/nextflow.config b/modules/nf-core/fastp/tests/nextflow.config new file mode 100644 index 00000000..0f7849ad --- /dev/null +++ b/modules/nf-core/fastp/tests/nextflow.config @@ -0,0 +1,6 @@ +process { + + withName: FASTP { + ext.args = "--interleaved_in" + } +} diff --git a/modules/nf-core/fastp/tests/tags.yml b/modules/nf-core/fastp/tests/tags.yml new file mode 100644 index 00000000..c1afcce7 --- /dev/null +++ b/modules/nf-core/fastp/tests/tags.yml @@ -0,0 +1,2 @@ +fastp: + - modules/nf-core/fastp/** diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml new file mode 100644 index 00000000..1787b38a --- /dev/null +++ b/modules/nf-core/fastqc/environment.yml @@ -0,0 +1,7 @@ +name: fastqc +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::fastqc=0.12.1 diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf new file mode 100644 index 00000000..9e19a74c --- /dev/null +++ b/modules/nf-core/fastqc/main.nf @@ -0,0 +1,55 @@ +process FASTQC { + tag "$meta.id" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0' : + 'biocontainers/fastqc:0.12.1--hdfd78af_0' }" + + input: + tuple val(meta), path(reads) + + output: + tuple val(meta), path("*.html"), emit: html + tuple val(meta), path("*.zip") , emit: zip + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + // Make list of old name and new name pairs to use for renaming in the bash while loop + def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] } + def rename_to = old_new_pairs*.join(' ').join(' ') + def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ') + """ + printf "%s %s\\n" $rename_to | while read old_name new_name; do + [ -f "\${new_name}" ] || ln -s \$old_name \$new_name + done + + fastqc \\ + $args \\ + --threads $task.cpus \\ + $renamed_files + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.html + touch ${prefix}.zip + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) + END_VERSIONS + """ +} diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml new file mode 100644 index 00000000..ee5507e0 --- /dev/null +++ b/modules/nf-core/fastqc/meta.yml @@ -0,0 +1,57 @@ +name: fastqc +description: Run FastQC on sequenced reads +keywords: + - quality control + - qc + - adapters + - fastq +tools: + - fastqc: + description: | + FastQC gives general quality metrics about your reads. + It provides information about the quality score distribution + across your reads, the per base sequence content (%A/C/G/T). + You get information about adapter contamination and other + overrepresented sequences. + homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ + documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/ + licence: ["GPL-2.0-only"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - html: + type: file + description: FastQC report + pattern: "*_{fastqc.html}" + - zip: + type: file + description: FastQC report archive + pattern: "*_{fastqc.zip}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" + - "@grst" + - "@ewels" + - "@FelixKrueger" +maintainers: + - "@drpatelh" + - "@grst" + - "@ewels" + - "@FelixKrueger" diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test new file mode 100644 index 00000000..70edae4d --- /dev/null +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -0,0 +1,212 @@ +nextflow_process { + + name "Test Process FASTQC" + script "../main.nf" + process "FASTQC" + + tag "modules" + tag "modules_nfcore" + tag "fastqc" + + test("sarscov2 single-end [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [ id: 'test', single_end:true ], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. + // looks like this:
    Mon 2 Oct 2023
    test.gz
    + // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    \n"; + print "\n"; + if ($low) + { + foreach my $threshold (sort {$a <=> $b} keys %bundleColorsRGBLow) + { + my $cellBgColor = rgbToHex($bundleColorsRGBLow{$threshold}); + print qq{\n}; + } + } + else + { + foreach my $threshold (sort {$a <=> $b} keys %bundleColorsRGB) + { + my $cellBgColor = rgbToHex($bundleColorsRGB{$threshold}); + print qq{\n}; + } + } + print "
    Bundled LinksRGB
    $threshold$bundleColorsRGBLow{$threshold}
    $threshold$bundleColorsRGB{$threshold}
    \n"; + exit(0); +} + +sub exportAsHTMLKeyTable { + print "\n"; + print "\n"; + if ($low) + { + foreach my $threshold (sort {$a <=> $b} keys %bundleColorsRGBLow) + { + my $cellBgColor = rgbToHex($bundleColorsRGBLow{$threshold}); + print qq{\n}; + } + } + else + { + foreach my $threshold (sort {$a <=> $b} keys %bundleColorsRGB) + { + my $cellBgColor = rgbToHex($bundleColorsRGB{$threshold}); + print qq{\n}; + } + } + print "
    Bundled Links
     $threshold
     $threshold
    \n"; + exit(0); +} +sub rgbToHex { + my ($r, $g, $b) = split/,/, $_[0]; + return sprintf ("#%2.2X%2.2X%2.2X", $r, $g, $b); +} + +my $bundleFileIn = ""; +my $bundleFileOut = ""; + +(@ARGV) or usage(); +foreach my $arg (@ARGV) +{ + ($arg =~ m/^-(h|help)$/) and usage(); + ($arg =~ m/^-low$/) and $low = 1; + ($arg =~ m/^-colorsRGB$/) and exportRGB(); + ($arg =~ m/^-colorsRGBAsHTMLTable$/) and exportRGBHTMLTable(); + ($arg =~ m/^-colorsHexAsHTMLKeyTable$/) and exportAsHTMLKeyTable(); + ($arg =~ m/^-i=(.+)$/) and $bundleFileIn = $1; + ($arg =~ m/^-o=(.+)$/) and $bundleFileOut = $1; +} + +open(OUT, ">$bundleFileOut") or die "ERROR: can not open bundle out file $bundleFileOut $!\n"; +open(IN, "<$bundleFileIn") or die "ERROR: can not open bundle in file $bundleFileIn $!\n"; +while (my $line = ) +{ +#ASB_LG19 13470754 14218750 Ss262 2177839 2976275 nlinks=672,bsize1=150447,bsize2=150419,bidentity1=0.201133,bidentity2=0.188392,depth1=0,depth2=0, +#ASB_LG19 14250080 15061508 Ss262 1303606 2191377 nlinks=1076,bsize1=279892,bsize2=278553,bidentity1=0.344937,bidentity2=0.313766,depth1=0,depth2=0, +#ASB_LG19 14314359 14314420 Ss262 7198136 7198167 nlinks=9,bsize1=62,bsize2=32,bidentity1=1.000000,bidentity2=1.000000,depth1=1,depth2=1, +#ASB_LG19 15064224 15625360 Ss262 672993 1254783 nlinks=881,bsize1=305520,bsize2=304727,bidentity1=0.544466,bidentity2=0.523774,depth1=0,depth2=0, +#ASB_LG19 15650721 16282135 Ss262 8995 672359 nlinks=786,bsize1=199405,bsize2=198505,bidentity1=0.315807,bidentity2=0.299239,depth1=0,depth2=0, +#ASB_LG19 17026943 17042421 Ss262 965 7848 nlinks=35,bsize1=7610,bsize2=4363,bidentity1=0.491634,bidentity2=0.633788,depth1=0,depth2=0, + chomp $line; + my @data = split/\s+/, $line; + my @bundleFields = split/,/, $data[6]; + my ($label, $count) = split/=/, $bundleFields[0]; + my $colorText = "color=(168,191,0)"; + if ($low) + { + foreach my $threshold (sort {$a <=> $b} keys %bundleColorsRGBLow) + { + if ($count > $threshold) + { + $colorText = "color=($bundleColorsRGBLow{$threshold})"; + } + } + } + else + { + foreach my $threshold (sort {$a <=> $b} keys %bundleColorsRGB) + { + if ($count > $threshold) + { + $colorText = "color=($bundleColorsRGB{$threshold})"; + } + } + } + my $newline = join(" ", $data[0], $data[1], $data[2], $data[3], $data[4], $data[5], $colorText, $data[6]); + select OUT; print OUT "$newline\n"; +} +close(OUT); +exit(0); diff --git a/bin/validateseqlists.sh b/bin/validateseqlists.sh new file mode 100755 index 00000000..959c87d9 --- /dev/null +++ b/bin/validateseqlists.sh @@ -0,0 +1,39 @@ +#!/usr/bin/env bash + +seqFileA=$1 +seqFileB=$2 + +linesFileA=() +linesFileB=() + +while IFS= read -r line; do + linesFileA+=("$line") + columns=($line) + if [[ ${#columns[@]} -ne 2 ]]; then + echo "Error: Sequence file $(basename "$seqFileA") does not have exactly two columns." >&2 + exit 1 + fi +done < "$seqFileA" + +while IFS= read -r line; do + linesFileB+=("$line") + columns=($line) + if [[ ${#columns[@]} -ne 2 ]]; then + echo "Error: Sequence file $(basename "$seqFileB") does not have exactly two columns." >&2 + exit 1 + fi +done < "$seqFileB" + +outputLines=("${linesFileA[@]}" "${linesFileB[@]}") + +secondColumn=() +for line in "${outputLines[@]}"; do + columns=($line) + secondColumn+=("${columns[1]}") +done + +uniqueSecondColumn=($(echo "${secondColumn[@]}" | tr ' ' '\n' | sort -u)) +if [[ ${#secondColumn[@]} -ne ${#uniqueSecondColumn[@]} ]]; then + echo "Error: Duplicate sequence labels detected in second column for pair: $(basename "$seqFileA"), $(basename "$seqFileB")" >&2 + exit 1 +fi diff --git a/conf/base.config b/conf/base.config index 895cad8a..c632c1ef 100644 --- a/conf/base.config +++ b/conf/base.config @@ -71,6 +71,9 @@ process { withName:SAMBLASTER { time = { check_max( 20.h * task.attempt, 'time' ) } } + withName:DNADIFF { + time = { check_max( 7.day * task.attempt, 'time' ) } + } withName:CUSTOM_DUMPSOFTWAREVERSIONS { cache = false } diff --git a/modules/local/circos.nf b/modules/local/circos.nf new file mode 100644 index 00000000..8311e694 --- /dev/null +++ b/modules/local/circos.nf @@ -0,0 +1,151 @@ +process CIRCOS { + tag "${target_on_ref_seq}" + label 'process_single' + + container "docker.io/gallvp/circos-tools:v0.23-1_ps" + + input: + tuple val(target_on_ref_seq), path(karyotype), path(bundle_file) + + output: + path "*.svg", emit: svg_file + path "*.png", emit: png_file + path "bundled.links.tsv", emit: bundled_links_tsv + path "circos.conf", emit: circos_conf + path "karyotype.tsv", emit: karyotype_tsv + path "versions.yml", emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + """ + links_count=\$(wc -l < "$bundle_file") + max_links=20000 + if [ "\$links_count" -gt "\$max_links" ]; then + echo "Link count exceeded \$max_links for ${bundle_file}." + echo "Try to shrink the number of links by increasing the max_gap and min_bundle_size options in the config file." + exit 1 + fi + + cat $karyotype > "karyotype.tsv" + cat $bundle_file | awk '{print \$1,\$2,\$3,\$4,\$5,\$6,\$7}' OFS="\\t" > bundled.links.tsv + + num_sequences=\$(cat $karyotype | wc -l) + if (( \$num_sequences <= 10 )); then + label_font_size=40 + elif (( \$num_sequences <= 30 )); then + label_font_size=30 + else + label_font_size=15 + fi + + if (( \$num_sequences <= 10 )); then + ticks_config=" + radius = dims(ideogram,radius_outer) + orientation = out + label_multiplier = 1e-6 + color = black + thickness = 5p + label_offset = 5p + + spacing = 0.5u + size = 10p + show_label = yes + label_size = 20p + format = %.1f + + + spacing = 1.0u + size = 15p + show_label = yes + label_size = 30p + format = %.1f + + " + + label_offset=" + 120p" + else + ticks_config="" + + label_offset=" + 25p" + fi + + cat <<-END_CONF > circos.conf + # circos.conf + karyotype = $karyotype + + + + default = 0.005r + + + radius = 0.8r + thickness = 25p + fill = yes + stroke_thickness = 0 + + show_label = yes + label_font = default + label_radius = dims(ideogram,radius_outer)\$label_offset + label_size = \$label_font_size + label_parallel = yes + + + + radius = 0.99r + crest = 1 + ribbon = yes + flat = yes + stroke_thickness = 0 + color = grey_a3 + + bezier_radius = 0r + bezier_radius_purity = 0.5 + + file = bundled.links.tsv + + + + show_ticks = yes + show_tick_labels = yes + chromosomes_units = 1000000 + chromosomes_display_default = yes + + \$ticks_config + + + <> + + <> + <> + END_CONF + + circos + + mv circos.svg "${target_on_ref_seq}.svg" + mv circos.png "${target_on_ref_seq}.png" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + circos: \$(circos -version | awk '{print \$2}' FS='|' | tr -d '[:space:]') + perl: \$(circos -version | awk '{print \$4}' FS='|' | tr -d '[:space:]Perl') + END_VERSIONS + """ + + stub: + """ + touch ${target_on_ref_seq}.svg + touch ${target_on_ref_seq}.png + + touch bundled.links.tsv + touch circos.conf + touch karyotype.tsv + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + circos: \$(circos -version | awk '{print \$2}' FS='|' | tr -d '[:space:]') + perl: \$(circos -version | awk '{print \$4}' FS='|' | tr -d '[:space:]Perl') + END_VERSIONS + """ +} diff --git a/modules/local/circos_bundlelinks.nf b/modules/local/circos_bundlelinks.nf new file mode 100644 index 00000000..d8f5d9f8 --- /dev/null +++ b/modules/local/circos_bundlelinks.nf @@ -0,0 +1,39 @@ +process CIRCOS_BUNDLELINKS { + tag "${target_on_ref}" + label 'process_single' + + container "docker.io/gallvp/circos-tools:v0.23-1_ps" + + input: + tuple val(target_on_ref), path(coords_file), path(report_file) + val max_gap + val min_bundle_size + + output: + tuple val(target_on_ref), path("*.xcoords.bundle.txt") , emit: links + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def VERSION='24Sep2013' + """ + cat \\ + $coords_file \\ + | awk '{print \$12,\$1,\$2,\$13,\$3,\$4}' OFS="\\t" \\ + > "\$(basename $coords_file).links.txt" + + /usr/share/circos/tools/bundlelinks/bin/bundlelinks \\ + -links "\$(basename $coords_file).links.txt" \\ + -max_gap $max_gap \\ + -min_bundle_size $min_bundle_size \\ + 1> "\$(basename $coords_file).bundle.txt" \\ + 2> bundlelinks.err + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bundlelinks: $VERSION + END_VERSIONS + """ +} diff --git a/modules/local/colourbundlelinks.nf b/modules/local/colourbundlelinks.nf new file mode 100644 index 00000000..125a747f --- /dev/null +++ b/modules/local/colourbundlelinks.nf @@ -0,0 +1,48 @@ +process COLOURBUNDLELINKS { + tag "${target_on_ref}" + label 'process_single' + + container "docker.io/gallvp/python3npkgs:v0.4" + + input: + tuple val(target_on_ref), path(bundle_links) + val color_by_contig + + output: + tuple val(target_on_ref), path("*.xcoords.bundle.coloured.txt") , emit: coloured_links + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def color_by_contig_bash = color_by_contig ? '1' : '0' + """ + if [[ "$color_by_contig_bash" = "1" ]];then + colorbundlesbycontig.py \\ + "${bundle_links}" \\ + > "\$(basename $bundle_links .bundle.txt).bundle.coloured.txt" + else + colorbundlesbysize.pl \\ + -i="${bundle_links}" \\ + -o="\$(basename $bundle_links .bundle.txt).bundle.coloured.txt" + fi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + python: \$(python --version | tr -d 'Python[:space:]') + perl: \$(perl --version |& sed -n 's/.*v\\([0-9]\\+\\.[0-9]\\+\\.[0-9]\\+\\).*/\\1/p') + END_VERSIONS + """ + + stub: + """ + touch "\$(basename $bundle_links .bundle.txt).bundle.coloured.txt" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + python: \$(python --version | tr -d 'Python[:space:]') + perl: \$(perl --version |& sed -n 's/.*v\\([0-9]\\+\\.[0-9]\\+\\.[0-9]\\+\\).*/\\1/p') + END_VERSIONS + """ +} diff --git a/modules/local/dnadiff.nf b/modules/local/dnadiff.nf new file mode 100644 index 00000000..772244e6 --- /dev/null +++ b/modules/local/dnadiff.nf @@ -0,0 +1,53 @@ +process DNADIFF { + tag "${target_on_ref}" + label 'process_single' + + container "docker.io/staphb/mummer:4.0.0" + + input: + tuple val(target_on_ref), path(target_fasta), path(ref_fasta), path(dnadiff_file) + val many_to_many_align + + output: + tuple val(target_on_ref), path("*.xcoords"), path("*.report") , emit: coords + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def inter_extension = many_to_many_align ? 'mcoords' : '1coords' + def out_extension = many_to_many_align ? 'm.xcoords' : '1.xcoords' + """ + cat \\ + $dnadiff_file \\ + | sed '1s/.*/${ref_fasta} ${target_fasta}/' \\ + > ${target_on_ref}.sed.delta + + dnadiff \\ + -p $target_on_ref \\ + -d ${target_on_ref}.sed.delta + + cat \\ + "${target_on_ref}.${inter_extension}" \\ + > "${target_on_ref}.${out_extension}" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + dnadiff: \$(dnadiff -v |& sed -n '/DNAdiff version/ s/DNAdiff version //p') + END_VERSIONS + """ + + stub: + def inter_extension = many_to_many_align ? 'mcoords' : '1coords' + def out_extension = many_to_many_align ? 'm.xcoords' : '1.xcoords' + """ + touch "${target_on_ref}.${out_extension}" + touch "${target_on_ref}.report" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + dnadiff: \$(dnadiff -v |& sed -n '/DNAdiff version/ s/DNAdiff version //p') + END_VERSIONS + """ +} diff --git a/modules/local/filtersortfasta.nf b/modules/local/filtersortfasta.nf new file mode 100644 index 00000000..4d291024 --- /dev/null +++ b/modules/local/filtersortfasta.nf @@ -0,0 +1,53 @@ +process FILTERSORTFASTA { + tag "${target}.on.${reference}" + label 'process_single' + + container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + 'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1': + 'biocontainers/samtools:1.16.1--h6899075_1' }" + + input: + tuple val(target), path(target_fasta), path(target_txt), val(reference), path(ref_fasta), path(ref_txt) + + output: + tuple val(target), val(reference), path("filtered.ordered.target.fasta"), path("filtered.ordered.ref.fasta"), emit: fasta + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + """ + validateseqlists.sh \\ + "$target_txt" \\ + "$ref_txt" + + samtools \\ + faidx \\ + $target_fasta \\ + \$(awk '{print \$1}' $target_txt) \\ + > filtered.ordered.target.fasta + + samtools \\ + faidx \\ + $ref_fasta \\ + \$(awk '{print \$1}' $ref_txt) \\ + > filtered.ordered.ref.fasta + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(samtools --version | sed -n '1s/samtools//p') + END_VERSIONS + """ + + stub: + """ + touch filtered.ordered.target.fasta + touch filtered.ordered.ref.fasta + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(samtools --version | sed -n '1s/samtools//p') + END_VERSIONS + """ +} diff --git a/modules/local/generatekaryotype.nf b/modules/local/generatekaryotype.nf new file mode 100644 index 00000000..905a0d81 --- /dev/null +++ b/modules/local/generatekaryotype.nf @@ -0,0 +1,59 @@ +process GENERATEKARYOTYPE { + tag "${target_on_ref}.${seq_tag}" + label 'process_single' + + container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + 'https://depot.galaxyproject.org/singularity/ubuntu:20.04': + 'nf-core/ubuntu:20.04' }" + + input: + tuple val(target_on_ref), val(seq_tag), path(split_bundle_file), path(target_seq_len), path(ref_seq_len) + + output: + tuple val("${target_on_ref}.${seq_tag}"), path("*.karyotype") , emit: karyotype + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + """ + cat <<-END_VERSIONS > versions.yml + "${task.process}": + awk: \$(awk --version | sed -n 's/awk version //p') + grep: \$(grep --version | sed -n 's/grep (BSD grep, GNU compatible) //p') + sed: \$(sed --version | sed -n 's/^sed //p') + END_VERSIONS + + + ref_seqs=(\$(awk '{print \$1}' $split_bundle_file | sort | uniq)) + + if [ \${#ref_seqs[@]} -eq 0 ]; then + touch "${target_on_ref}.${seq_tag}.karyotype" + exit 0 + fi + + tmp_file=\$(mktemp) + printf '%s\\n' "\${ref_seqs[@]}" > "\$tmp_file" + + if [[ $seq_tag = "all" ]];then + cat $target_seq_len > filtered.target.seq.len + else + grep -w "$seq_tag" $target_seq_len > filtered.target.seq.len + fi + cat filtered.target.seq.len | awk '{print \$1,\$2,"grey"}' OFS="\\t" > colored.filtered.target.seq.len + + grep -w -f "\$tmp_file" $ref_seq_len > filtered.ref.seq.len + cat filtered.ref.seq.len | awk '{print \$1,\$2,"black"}' OFS="\\t" > colored.filtered.ref.seq.len + + cat colored.filtered.ref.seq.len | sort -k1V > merged.seq.lengths + cat colored.filtered.target.seq.len | sort -k1Vr >> merged.seq.lengths + sed -i '/^\$/d' merged.seq.lengths + + cat merged.seq.lengths \ + | awk '{print "chr -",\$1,\$1,"0",\$2-1,\$3}' OFS="\\t" \ + > "${target_on_ref}.${seq_tag}.karyotype" + + rm "\$tmp_file" + """ +} diff --git a/modules/local/getfastalength.nf b/modules/local/getfastalength.nf new file mode 100644 index 00000000..3f3748d7 --- /dev/null +++ b/modules/local/getfastalength.nf @@ -0,0 +1,55 @@ +process GETFASTALENGTH { + tag "${target}.on.${reference}" + label 'process_single' + + container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + 'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1': + 'biocontainers/samtools:1.16.1--h6899075_1' }" + + input: + tuple val(target), val(reference), path(filtered_ordered_target_fasta), path(filtered_ordered_ref_fasta) + + output: + tuple val("${target}.on.${reference}"), path("target.seq.lengths"), path("ref.seq.lengths") , emit: length + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + """ + samtools \\ + faidx \\ + $filtered_ordered_target_fasta + + samtools \\ + faidx \\ + $filtered_ordered_ref_fasta + + cat \\ + "${filtered_ordered_target_fasta}.fai"\\ + | awk '{print \$1, \$2}' OFS="\\t" \\ + > target.seq.lengths + + cat \\ + "${filtered_ordered_ref_fasta}.fai" \\ + | awk '{print \$1, \$2}' OFS="\\t" \\ + > ref.seq.lengths + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(samtools --version | sed -n '1s/samtools//p') + END_VERSIONS + """ + + stub: + """ + touch target.seq.lengths + touch ref.seq.lengths + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(samtools --version | sed -n '1s/samtools//p') + END_VERSIONS + """ +} diff --git a/modules/local/mummer.nf b/modules/local/mummer.nf new file mode 100644 index 00000000..401dbaa1 --- /dev/null +++ b/modules/local/mummer.nf @@ -0,0 +1,41 @@ +process MUMMER { + tag "${target}.on.${reference}" + label 'process_high' + + container "docker.io/staphb/mummer:4.0.0" + + input: + tuple val(target), val(reference), path(target_fasta), path(ref_fasta) + + output: + tuple val("${target}.on.${reference}"), path("*.delta") , emit: delta + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + """ + nucmer \ + --mum \\ + -t ${task.cpus} \\ + -p "${target}.on.${reference}" \\ + $ref_fasta \\ + $target_fasta + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + nucmer: \$(nucmer -V) + END_VERSIONS + """ + + stub: + """ + touch "${target}.on.${reference}.delta" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + nucmer: \$(nucmer -V) + END_VERSIONS + """ +} diff --git a/modules/local/relabelbundlelinks.nf b/modules/local/relabelbundlelinks.nf new file mode 100644 index 00000000..99bb27e7 --- /dev/null +++ b/modules/local/relabelbundlelinks.nf @@ -0,0 +1,51 @@ +process RELABELBUNDLELINKS { + tag "${target_on_ref}" + label 'process_single' + + container "docker.io/gallvp/python3npkgs:v0.4" + + input: + tuple val(target_on_ref), path(coloured_bundle_links), path(target_seq_list), path(ref_seq_list) + + output: + tuple val(target_on_ref), path("*.xcoords.bundle.coloured.relabeled.txt") , emit: relabeled_links + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + """ + #!/usr/bin/env python + + import sys + import os + import pandas as pd + from platform import python_version + + # Write versions + with open(f"versions.yml", "w") as f_versions: + f_versions.write('"${task.process}":\\n') + f_versions.write(f" python: {python_version()}\\n") + + output_file_name = ".".join("$coloured_bundle_links".split(".")[0:-1]) + ".relabeled.txt" + + subs_target_seq = pd.read_csv('$target_seq_list', sep='\\t', header=None) + subs_target_seq_dict = dict(zip(subs_target_seq.iloc[:, 0], subs_target_seq.iloc[:, 1])) + + subs_ref_seq = pd.read_csv('$ref_seq_list', sep='\\t', header=None) + subs_ref_seq_dict = dict(zip(subs_ref_seq.iloc[:, 0], subs_ref_seq.iloc[:, 1])) + + if os.path.getsize('$coloured_bundle_links') == 0: + with open(output_file_name, 'w') as f: + f.write('') + sys.exit(0) + else: + df = pd.read_csv('$coloured_bundle_links', sep=' ', header=None) + + df.iloc[:, 3] = df.iloc[:, 3].replace(subs_target_seq_dict, regex=False) + df.iloc[:, 0] = df.iloc[:, 0].replace(subs_ref_seq_dict, regex=False) + + df.to_csv(output_file_name, sep=' ', index=False, header=None) + """ +} diff --git a/modules/local/relabelfastalength.nf b/modules/local/relabelfastalength.nf new file mode 100644 index 00000000..74abe98e --- /dev/null +++ b/modules/local/relabelfastalength.nf @@ -0,0 +1,54 @@ +process RELABELFASTALENGTH { + tag "${target_on_ref}" + label 'process_single' + + container "docker.io/gallvp/python3npkgs:v0.4" + + input: + tuple val(target_on_ref), path(target_seq_lengths), path(ref_seq_lengths), path(target_seq_list), path(ref_seq_list) + + output: + tuple val(target_on_ref), path("relabeld.target.seq.lengths"), path("relabeld.ref.seq.lengths") , emit: relabeled_seq_lengths + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + """ + #!/usr/bin/env python + + import pandas as pd + from platform import python_version + + subs_target_seq = pd.read_csv('$target_seq_list', sep='\\t', header=None) + subs_target_seq_dict = dict(zip(subs_target_seq.iloc[:, 0], subs_target_seq.iloc[:, 1])) + + subs_ref_seq = pd.read_csv('$ref_seq_list', sep='\\t', header=None) + subs_ref_seq_dict = dict(zip(subs_ref_seq.iloc[:, 0], subs_ref_seq.iloc[:, 1])) + + df_target_seq_lengths = pd.read_csv('$target_seq_lengths', sep='\\t', header=None) + df_target_seq_lengths.iloc[:, 0] = df_target_seq_lengths.iloc[:, 0].replace(subs_target_seq_dict, regex=False) + df_target_seq_lengths.to_csv("relabeld.target.seq.lengths", sep='\\t', index=False, header=None) + + df_ref_seq_lengths = pd.read_csv('$ref_seq_lengths', sep='\\t', header=None) + df_ref_seq_lengths.iloc[:, 0] = df_ref_seq_lengths.iloc[:, 0].replace(subs_ref_seq_dict, regex=False) + df_ref_seq_lengths.to_csv("relabeld.ref.seq.lengths", sep='\\t', index=False, header=None) + + # Write versions + with open(f"versions.yml", "w") as f_versions: + f_versions.write('"${task.process}":\\n') + f_versions.write(f" python: {python_version()}\\n") + """ + + stub: + """ + touch relabeld.target.seq.lengths + touch relabeld.ref.seq.lengths + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + python: \$(python --version | tr -d 'Python[:space:]') + END_VERSIONS + """ +} diff --git a/modules/local/splitbundlefile.nf b/modules/local/splitbundlefile.nf new file mode 100644 index 00000000..c9cb4358 --- /dev/null +++ b/modules/local/splitbundlefile.nf @@ -0,0 +1,42 @@ +process SPLITBUNDLEFILE { + tag "${target_on_ref}" + label 'process_single' + + container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + 'https://depot.galaxyproject.org/singularity/ubuntu:20.04': + 'nf-core/ubuntu:20.04' }" + + input: + tuple val(target_on_ref), path(coloured_bundle_links) + val plot_1_vs_all + + output: + tuple val(target_on_ref), path("*.split.bundle.txt"), emit: split_file + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def plot_1_vs_all_bash = plot_1_vs_all ? '1' : '0' + """ + if [[ "$plot_1_vs_all_bash" = "1" ]];then + target_seqs=(\$(awk '{print \$4}' $coloured_bundle_links | sort | uniq)) + + for i in "\${!target_seqs[@]}" + do + target_seq=\${target_seqs[\$i]} + awk -v seq="\$target_seq" '\$4==seq {print \$0}' $coloured_bundle_links > "${target_on_ref}.\${target_seq}.split.bundle.txt" + done + fi + + cat \\ + $coloured_bundle_links \\ + > "${target_on_ref}.all.split.bundle.txt" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + awk: \$(awk --version | sed -n 's/awk version //p') + END_VERSIONS + """ +} diff --git a/nextflow.config b/nextflow.config index cf50c3f7..97372502 100644 --- a/nextflow.config +++ b/nextflow.config @@ -51,12 +51,12 @@ params { // Synteny options synteny_skip = true synteny_between_input_assemblies = true - synteny_xref_assemblies = null synteny_many_to_many_align = false synteny_max_gap = 1000000 synteny_min_bundle_size = 1000 synteny_plot_1_vs_all = true synteny_color_by_contig = true + synteny_xref_assemblies = null // Output options outdir = './results' @@ -256,7 +256,7 @@ dag { manifest { name = 'plant-food-research-open/assemblyqc' - author = """Usman Rashid""" + author = """Usman Rashid, Ken Smith, Ross Crowhurst, Chen Wu, Marcus Davy""" homePage = 'https://github.com/plant-food-research-open/assemblyqc' description = """A NextFlow pipeline which evaluates assembly quality with well-established tools and presents the results in a unified html report.""" mainScript = 'main.nf' diff --git a/nextflow_schema.json b/nextflow_schema.json index fcbfcbd1..69f67e0a 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -220,14 +220,6 @@ "description": "Create syntenic plots between each pair of input assemblies", "default": true }, - "synteny_xref_assemblies": { - "type": "string", - "description": "Reference assemblies for synteny analysis", - "help_text": "FASTA and synteny label tsv files should be provided in a formatted CSV file ", - "format": "file-path", - "mimetype": "csv", - "schema": "assets/schema_xref_assemblies.json" - }, "synteny_many_to_many_align": { "type": "boolean", "description": "Include alignment blocks with many-to-many mappings (dnadiff .mcoords file)" @@ -251,6 +243,14 @@ "type": "boolean", "default": true, "description": "Synteny plot is colored by contig" + }, + "synteny_xref_assemblies": { + "type": "string", + "description": "Reference assemblies for synteny analysis", + "help_text": "FASTA and synteny label tsv files should be provided in a formatted CSV file ", + "format": "file-path", + "mimetype": "csv", + "schema": "assets/schema_xref_assemblies.json" } } }, diff --git a/subworkflows/local/fasta_synteny.nf b/subworkflows/local/fasta_synteny.nf new file mode 100644 index 00000000..219b3da7 --- /dev/null +++ b/subworkflows/local/fasta_synteny.nf @@ -0,0 +1,253 @@ +include { GUNZIP as GUNZIP_FASTA } from '../../modules/nf-core/gunzip/main' +include { FILTERSORTFASTA } from '../../modules/local/filtersortfasta' +include { MUMMER } from '../../modules/local/mummer' +include { GETFASTALENGTH } from '../../modules/local/getfastalength' +include { DNADIFF } from '../../modules/local/dnadiff' +include { CIRCOS_BUNDLELINKS } from '../../modules/local/circos_bundlelinks' +include { COLOURBUNDLELINKS } from '../../modules/local/colourbundlelinks' +include { RELABELBUNDLELINKS } from '../../modules/local/relabelbundlelinks' +include { SPLITBUNDLEFILE } from '../../modules/local/splitbundlefile' +include { RELABELFASTALENGTH } from '../../modules/local/relabelfastalength' +include { GENERATEKARYOTYPE } from '../../modules/local/generatekaryotype' +include { CIRCOS } from '../../modules/local/circos' + +workflow FASTA_SYNTENY { + take: + ch_fasta // Channel: [ tag, fa ] + ch_labels // Channel: [ tag, txt ] + ch_xref_fasta_labels // Channel: [ tag2, fa, txt ] + between_input_assemblies // val(true|false) + many_to_many_align // val(true|false) + max_gap // val(Integer) + min_bundle_size // val(Integer) + plot_1_vs_all // val(true|false) + color_by_contig // val(true|false) + + main: + ch_versions = Channel.empty() + + ch_fasta_labels = ch_fasta + | join( + ch_labels + ) + + ch_input_combinations = ! between_input_assemblies + ? Channel.empty() + : ch_fasta_labels + | map { [it] } + | collect + | map { getUniqueWithinCombinations(it) } + | flatten + | buffer(size:6) + + ch_xref_fa_branch = ch_xref_fasta_labels + | map { tag, fa, txt -> + [ [ id: tag ], fa ] + } + | branch { meta, fa -> + gz: "$fa".endsWith(".gz") + rest: !"$fa".endsWith(".gz") + } + + // MODULE: GUNZIP_FASTA + GUNZIP_FASTA ( ch_xref_fa_branch.gz ) + + ch_xref_ungz_fa_labels = GUNZIP_FASTA.out.gunzip + | mix( + ch_xref_fa_branch.rest + ) + | map { meta, fa -> [ meta.id, fa ] } + | join( + ch_xref_fasta_labels + ) + | map { tag, fa, input_fa, seq_list -> + [ tag, fa, seq_list ] + } + + ch_all_combinations = ch_input_combinations + | mix( + ch_fasta_labels + | combine( + ch_xref_ungz_fa_labels + ) + ) + + ch_all_combination_labels = ch_all_combinations + | map { target_tag, target_fa, target_txt, xref_tag, xref_fa, xref_txt -> + [ "${target_tag}.on.${xref_tag}", target_txt, xref_txt ] + } + + ch_versions = ch_versions.mix(GUNZIP_FASTA.out.versions.first()) + + // MODULE: FILTERSORTFASTA + FILTERSORTFASTA ( ch_all_combinations ) + + ch_versions = ch_versions.mix(FILTERSORTFASTA.out.versions.first()) + + // MODULE: MUMMER + MUMMER ( FILTERSORTFASTA.out.fasta ) + + ch_versions = ch_versions.mix(MUMMER.out.versions.first()) + + // MODULE: GETFASTALENGTH + GETFASTALENGTH ( FILTERSORTFASTA.out.fasta ) + + ch_versions = ch_versions.mix(GETFASTALENGTH.out.versions.first()) + + // MODULE: DNADIFF + ch_dnadiff_inputs = FILTERSORTFASTA.out.fasta + | map { target, reference, target_fasta, ref_fasta -> + [ "${target}.on.${reference}", target_fasta, ref_fasta ] + } + | join( + MUMMER.out.delta + ) + DNADIFF( + ch_dnadiff_inputs, + many_to_many_align + ) + + ch_versions = ch_versions.mix(DNADIFF.out.versions.first()) + + // MODULE: CIRCOS_BUNDLELINKS + CIRCOS_BUNDLELINKS( + DNADIFF.out.coords, + max_gap, + min_bundle_size + ) + + ch_versions = ch_versions.mix(CIRCOS_BUNDLELINKS.out.versions.first()) + + // MODULE: COLOURBUNDLELINKS + COLOURBUNDLELINKS( + CIRCOS_BUNDLELINKS.out.links, + color_by_contig + ) + + ch_coloured_links = COLOURBUNDLELINKS.out.coloured_links + ch_versions = ch_versions.mix(COLOURBUNDLELINKS.out.versions.first()) + + // MODULE: RELABELBUNDLELINKS + ch_relabellinks_inputs = ch_coloured_links + | join(ch_all_combination_labels) + + RELABELBUNDLELINKS ( ch_relabellinks_inputs ) + + ch_versions = ch_versions.mix(RELABELBUNDLELINKS.out.versions.first()) + + // MODULE: SPLITBUNDLEFILE + SPLITBUNDLEFILE( + RELABELBUNDLELINKS.out.relabeled_links, + plot_1_vs_all + ) + + ch_split_links = SPLITBUNDLEFILE.out.split_file + | map { flattenSplitBundles(it) } + | flatten + | buffer(size:3) + + ch_versions = ch_versions.mix(SPLITBUNDLEFILE.out.versions.first()) + + // MODULE: RELABELFASTALENGTH + ch_relabelfastalength_inputs = GETFASTALENGTH.out.length + | join(ch_all_combination_labels) + + RELABELFASTALENGTH ( ch_relabelfastalength_inputs ) + + ch_versions = ch_versions.mix(RELABELFASTALENGTH.out.versions.first()) + + // MODULE: GENERATEKARYOTYPE + ch_generate_karyotype_inputs = RELABELFASTALENGTH.out.relabeled_seq_lengths + | cross( + ch_split_links + ) + | map { seq_len_tuple, split_bundle_tuple -> + + def target_on_xref = seq_len_tuple[0] + def seq_tag = split_bundle_tuple[1] + def split_bundle_file = split_bundle_tuple[2] + def target_seq_len = seq_len_tuple[1] + def ref_seq_len = seq_len_tuple[2] + + [ target_on_xref, seq_tag, split_bundle_file, target_seq_len, ref_seq_len ] + } + GENERATEKARYOTYPE ( ch_generate_karyotype_inputs ) + + ch_versions = ch_versions.mix(GENERATEKARYOTYPE.out.versions.first()) + + // MODULE: CIRCOS + ch_circos_inputs = GENERATEKARYOTYPE.out.karyotype + | join( + ch_split_links + | map { target_on_xref, seq_tag, txt -> + [ "${target_on_xref}.${seq_tag}", txt ] + } + ) + CIRCOS ( ch_circos_inputs ) + + ch_versions = ch_versions.mix(CIRCOS.out.versions.first()) + + emit: + plot = CIRCOS.out.png_file + versions = ch_versions +} + +def getUniqueWithinCombinations(inputArray) { + if (inputArray.size() <= 1) { + return [] + } + + inputArray.sort { a, b -> a[0].compareTo(b[0]) } + + def outputList = [] + + for (int i = 0; i < inputArray.size() - 1; i++) { + for (int j = i + 1; j < inputArray.size(); j++) { + def combination = [ + inputArray[i][0], + inputArray[i][1], + inputArray[i][2], + inputArray[j][0], + inputArray[j][1], + inputArray[j][2] + ] + outputList.add(combination) + } + } + return outputList +} + +def appendTags(tag, valuesArray) { + if (valuesArray.size() <= 1) { + return [] + } + + def outputList = [] + + for (int i = 0; i < valuesArray.size(); i++) { + outputList.add([tag, valuesArray[i]]) + } + return outputList +} + +def flattenSplitBundles(inputArray) { + def target_on_ref = inputArray[0] + def files = inputArray[1] + + if(files in ArrayList) { + return files.collect { [target_on_ref, extractBundleTag(it), it] } + } else { + return [files].collect { [target_on_ref, extractBundleTag(it), it] } + } +} + +def extractBundleTag(filePath) { + def regex = /.*\.(\w+)\.split\.bundle\.txt/ + def matcher = filePath =~ regex + if (matcher.matches()) { + return matcher.group(1) + } else { + // This branch should not execut unless the upstream logic is flawed + error "Error: Failed to parse the sequence tag from file name: ${filePath.getName()}" + } +} diff --git a/tests/stub/TT_2021a.seq.list b/tests/stub/TT_2021a.seq.labels.tsv similarity index 100% rename from tests/stub/TT_2021a.seq.list rename to tests/stub/TT_2021a.seq.labels.tsv diff --git a/tests/stub/stub.config b/tests/stub/stub.config index 5664ccc0..8f0f03b4 100644 --- a/tests/stub/stub.config +++ b/tests/stub/stub.config @@ -1,8 +1,8 @@ params { - config_profile_name = 'Full stub test' - config_profile_description = 'Full test of the pipeline in stub mode' + config_profile_name = 'Full stub test' + config_profile_description = 'Full test of the pipeline in stub mode' - input = 'https://raw.githubusercontent.com/Plant-Food-Research-Open/assemblyqc/dev/tests/stub/assemblysheet.csv' + input = 'tests/stub/assemblysheet.csv' ncbi_fcs_adaptor_skip = false @@ -22,6 +22,9 @@ params { hic = 'tests/stub/hic/Dummy_hic.R{1,2}.fq.gz' + synteny_skip = false + synteny_xref_assemblies = 'tests/stub/xrefsheet.csv' + // Limit resources so that this can run on GitHub Actions max_cpus = 2 max_memory = '6.GB' diff --git a/tests/stub/xrefsheet.csv b/tests/stub/xrefsheet.csv new file mode 100644 index 00000000..5c218707 --- /dev/null +++ b/tests/stub/xrefsheet.csv @@ -0,0 +1,2 @@ +tag,fasta,synteny_labels +TT_2021a,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/021/950/295/GCA_021950295.1_ASM2195029v1/GCA_021950295.1_ASM2195029v1_genomic.fna.gz,https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/tests/stub/TT_2021a.seq.labels.tsv diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index 9f521a0f..0854028e 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -36,6 +36,7 @@ include { FASTA_BUSCO_PLOT } from '../subworkflows/local/fasta_ include { FASTA_LTRRETRIEVER_LAI } from '../subworkflows/pfr/fasta_ltrretriever_lai/main' include { FASTA_KRAKEN2 } from '../subworkflows/local/fasta_kraken2' include { FQ2HIC } from '../subworkflows/local/fq2hic' +include { FASTA_SYNTENY } from '../subworkflows/local/fasta_synteny' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -97,6 +98,19 @@ workflow ASSEMBLYQC { : null } + ch_synteny_labels = ch_input + | map { tag, fasta, gff, mono_ids, labels -> + labels + ? [ [ id: tag ], file(labels, checkIfExists: true) ] + : ( + params.synteny_skip + ? null + : log.warn("A synteny_labels file must be provided" + + " in the input assembly sheet when running synteny analysis." + + " Synteny analysis is skipped!") + ) + } + ch_hic_reads = ! params.hic ? Channel.empty() : ( @@ -108,6 +122,13 @@ workflow ASSEMBLYQC { [ [ id: sample, single_end: false ], fq ] } + ch_xref_assembly = params.synteny_skip || ! params.synteny_xref_assemblies + ? Channel.empty() + : Channel.fromSamplesheet('synteny_xref_assemblies') + | map { tag, fa, labels -> + [ tag, file(fa, checkIfExists: true), file(labels, checkIfExists: true) ] + } + // MODULE: GUNZIP as GUNZIP_FASTA GUNZIP_FASTA ( ch_target_assemby_branch.gz ) @@ -380,6 +401,22 @@ workflow ASSEMBLYQC { ch_hic_html = FQ2HIC.out.html ch_versions = ch_versions.mix(FQ2HIC.out.versions) + // SUBWORKFLOW: FASTA_SYNTENY + FASTA_SYNTENY( + ch_clean_assembly, + ch_synteny_labels.map { meta, txt -> [ meta.id, txt ] }, + ch_xref_assembly, + params.synteny_between_input_assemblies, + params.synteny_many_to_many_align, + params.synteny_max_gap, + params.synteny_min_bundle_size, + params.synteny_plot_1_vs_all, + params.synteny_color_by_contig + ) + + ch_synteny_plot = FASTA_SYNTENY.out.plot + ch_versions = ch_versions.mix(FASTA_SYNTENY.out.versions) + // MODULE: CUSTOM_DUMPSOFTWAREVERSIONS CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') From a6eec71880e6da5376b0fe457b67f289edc8a29f Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 27 Feb 2024 19:50:48 +1300 Subject: [PATCH 32/65] Added createreport module --- bin/assemblyqc.py | 67 ++ .../parsers/assemblathon_stats_parser.py | 53 ++ bin/report_modules/parsers/busco_parser.py | 197 ++++++ bin/report_modules/parsers/circos_parser.py | 52 ++ .../parsers/fasta_validate_parser.py | 35 + .../parsers/genometools_gt_stat_parser.py | 344 ++++++++++ .../parsers/gff3_validate_parser.py | 35 + bin/report_modules/parsers/hic_parser.py | 34 + bin/report_modules/parsers/kraken2_parser.py | 34 + bin/report_modules/parsers/lai_parser.py | 95 +++ .../parsers/ncbi_fcs_adaptor_parser.py | 44 ++ .../parsers/ncbi_fcs_gx_parser.py | 56 ++ bin/report_modules/parsers/params_parser.py | 43 ++ bin/report_modules/parsers/parsing_commons.py | 10 + bin/report_modules/parsers/tidk_parser.py | 68 ++ bin/report_modules/parsers/tools_parser.py | 33 + bin/report_modules/report_printer.py | 15 + .../assemblathon_stats.html | 21 + .../assemblathon_stats/dropdown.html | 10 + .../assemblathon_stats/report_contents.html | 17 + bin/report_modules/templates/base.html | 68 ++ bin/report_modules/templates/busco/busco.html | 18 + .../templates/busco/dropdown.html | 13 + .../templates/busco/report_contents.html | 77 +++ .../templates/busco/summary_contents.html | 37 + .../templates/circos/circos.html | 32 + .../templates/circos/dropdown.html | 10 + .../templates/circos/report_contents.html | 26 + .../templates/fasta_validate/dropdown.html | 10 + .../fasta_validate/fasta_validate.html | 14 + .../fasta_validate/report_contents.html | 18 + .../genometools_gt_stat/dropdown.html | 10 + .../genometools_gt_stat.html | 15 + .../genometools_gt_stat/report_contents.html | 35 + .../templates/gff3_validate/dropdown.html | 10 + .../gff3_validate/gff3_validate.html | 22 + .../gff3_validate/report_contents.html | 18 + bin/report_modules/templates/header.html | 637 ++++++++++++++++++ .../templates/hic/dropdown.html | 10 + bin/report_modules/templates/hic/hic.html | 18 + .../templates/hic/report_contents.html | 17 + bin/report_modules/templates/js.html | 58 ++ .../templates/kraken2/dropdown.html | 11 + .../templates/kraken2/kraken2.html | 15 + .../templates/kraken2/report_contents.html | 21 + .../templates/lai/dropdown.html | 7 + bin/report_modules/templates/lai/lai.html | 26 + .../templates/lai/summary_contents.html | 30 + .../templates/ncbi_fcs_adaptor/dropdown.html | 10 + .../ncbi_fcs_adaptor/ncbi_fcs_adaptor.html | 11 + .../ncbi_fcs_adaptor/report_contents.html | 24 + .../templates/ncbi_fcs_gx/dropdown.html | 10 + .../templates/ncbi_fcs_gx/ncbi_fcs_gx.html | 23 + .../ncbi_fcs_gx/report_contents.html | 49 ++ .../templates/params/contents.html | 8 + .../templates/params/params.html | 19 + .../templates/tidk/dropdown.html | 11 + .../templates/tidk/report_contents.html | 33 + bin/report_modules/templates/tidk/tidk.html | 15 + .../templates/tools/contents.html | 9 + bin/report_modules/templates/tools/tools.html | 1 + conf/modules.config | 24 +- lib/WorkflowAssemblyqc.groovy | 31 +- lib/WorkflowMain.groovy | 20 - modules/local/createreport.nf | 38 ++ workflows/assemblyqc.nf | 19 + 66 files changed, 2864 insertions(+), 37 deletions(-) create mode 100755 bin/assemblyqc.py create mode 100644 bin/report_modules/parsers/assemblathon_stats_parser.py create mode 100644 bin/report_modules/parsers/busco_parser.py create mode 100644 bin/report_modules/parsers/circos_parser.py create mode 100644 bin/report_modules/parsers/fasta_validate_parser.py create mode 100644 bin/report_modules/parsers/genometools_gt_stat_parser.py create mode 100644 bin/report_modules/parsers/gff3_validate_parser.py create mode 100644 bin/report_modules/parsers/hic_parser.py create mode 100644 bin/report_modules/parsers/kraken2_parser.py create mode 100644 bin/report_modules/parsers/lai_parser.py create mode 100644 bin/report_modules/parsers/ncbi_fcs_adaptor_parser.py create mode 100644 bin/report_modules/parsers/ncbi_fcs_gx_parser.py create mode 100644 bin/report_modules/parsers/params_parser.py create mode 100644 bin/report_modules/parsers/parsing_commons.py create mode 100644 bin/report_modules/parsers/tidk_parser.py create mode 100644 bin/report_modules/parsers/tools_parser.py create mode 100644 bin/report_modules/report_printer.py create mode 100644 bin/report_modules/templates/assemblathon_stats/assemblathon_stats.html create mode 100644 bin/report_modules/templates/assemblathon_stats/dropdown.html create mode 100644 bin/report_modules/templates/assemblathon_stats/report_contents.html create mode 100644 bin/report_modules/templates/base.html create mode 100644 bin/report_modules/templates/busco/busco.html create mode 100644 bin/report_modules/templates/busco/dropdown.html create mode 100644 bin/report_modules/templates/busco/report_contents.html create mode 100644 bin/report_modules/templates/busco/summary_contents.html create mode 100644 bin/report_modules/templates/circos/circos.html create mode 100644 bin/report_modules/templates/circos/dropdown.html create mode 100644 bin/report_modules/templates/circos/report_contents.html create mode 100644 bin/report_modules/templates/fasta_validate/dropdown.html create mode 100644 bin/report_modules/templates/fasta_validate/fasta_validate.html create mode 100644 bin/report_modules/templates/fasta_validate/report_contents.html create mode 100644 bin/report_modules/templates/genometools_gt_stat/dropdown.html create mode 100644 bin/report_modules/templates/genometools_gt_stat/genometools_gt_stat.html create mode 100644 bin/report_modules/templates/genometools_gt_stat/report_contents.html create mode 100644 bin/report_modules/templates/gff3_validate/dropdown.html create mode 100644 bin/report_modules/templates/gff3_validate/gff3_validate.html create mode 100644 bin/report_modules/templates/gff3_validate/report_contents.html create mode 100644 bin/report_modules/templates/header.html create mode 100644 bin/report_modules/templates/hic/dropdown.html create mode 100644 bin/report_modules/templates/hic/hic.html create mode 100644 bin/report_modules/templates/hic/report_contents.html create mode 100644 bin/report_modules/templates/js.html create mode 100644 bin/report_modules/templates/kraken2/dropdown.html create mode 100644 bin/report_modules/templates/kraken2/kraken2.html create mode 100644 bin/report_modules/templates/kraken2/report_contents.html create mode 100644 bin/report_modules/templates/lai/dropdown.html create mode 100644 bin/report_modules/templates/lai/lai.html create mode 100644 bin/report_modules/templates/lai/summary_contents.html create mode 100644 bin/report_modules/templates/ncbi_fcs_adaptor/dropdown.html create mode 100644 bin/report_modules/templates/ncbi_fcs_adaptor/ncbi_fcs_adaptor.html create mode 100644 bin/report_modules/templates/ncbi_fcs_adaptor/report_contents.html create mode 100644 bin/report_modules/templates/ncbi_fcs_gx/dropdown.html create mode 100644 bin/report_modules/templates/ncbi_fcs_gx/ncbi_fcs_gx.html create mode 100644 bin/report_modules/templates/ncbi_fcs_gx/report_contents.html create mode 100644 bin/report_modules/templates/params/contents.html create mode 100644 bin/report_modules/templates/params/params.html create mode 100644 bin/report_modules/templates/tidk/dropdown.html create mode 100644 bin/report_modules/templates/tidk/report_contents.html create mode 100644 bin/report_modules/templates/tidk/tidk.html create mode 100644 bin/report_modules/templates/tools/contents.html create mode 100644 bin/report_modules/templates/tools/tools.html create mode 100644 modules/local/createreport.nf diff --git a/bin/assemblyqc.py b/bin/assemblyqc.py new file mode 100755 index 00000000..99eb2d73 --- /dev/null +++ b/bin/assemblyqc.py @@ -0,0 +1,67 @@ +#!/usr/bin/env python3 + +import json +import yaml + +from report_modules.report_printer import ReportPrinter + +from report_modules.parsers.params_parser import parse_params_json +from report_modules.parsers.tools_parser import parse_tools_yaml + +from report_modules.parsers.gff3_validate_parser import parse_gff3_validate_folder +from report_modules.parsers.fasta_validate_parser import parse_fasta_validate_folder + +from report_modules.parsers.ncbi_fcs_adaptor_parser import parse_ncbi_fcs_adaptor_folder +from report_modules.parsers.ncbi_fcs_gx_parser import parse_ncbi_fcs_gx_folder +from report_modules.parsers.assemblathon_stats_parser import ( + parse_assemblathon_stats_folder, +) +from report_modules.parsers.genometools_gt_stat_parser import ( + parse_genometools_gt_stat_folder, +) +from report_modules.parsers.busco_parser import parse_busco_folder +from report_modules.parsers.tidk_parser import parse_tidk_folder +from report_modules.parsers.lai_parser import parse_lai_folder +from report_modules.parsers.kraken2_parser import parse_kraken2_folder +from report_modules.parsers.hic_parser import parse_hic_folder +from report_modules.parsers.circos_parser import parse_circos_folder + +if __name__ == "__main__": + params_dict, params_table = parse_params_json() + tools_dict, tools_table = parse_tools_yaml() + + data_from_tools = {} + + data_from_tools = {**data_from_tools, **parse_gff3_validate_folder()} + data_from_tools = {**data_from_tools, **parse_fasta_validate_folder()} + data_from_tools = {**data_from_tools, **parse_ncbi_fcs_adaptor_folder()} + data_from_tools = {**data_from_tools, **parse_ncbi_fcs_gx_folder()} + data_from_tools = {**data_from_tools, **parse_assemblathon_stats_folder()} + data_from_tools = {**data_from_tools, **parse_genometools_gt_stat_folder()} + data_from_tools = {**data_from_tools, **parse_busco_folder()} + data_from_tools = {**data_from_tools, **parse_tidk_folder()} + data_from_tools = {**data_from_tools, **parse_lai_folder()} + data_from_tools = {**data_from_tools, **parse_kraken2_folder()} + data_from_tools = {**data_from_tools, **parse_hic_folder()} + data_from_tools = {**data_from_tools, **parse_circos_folder()} + + with open("software_versions.yml", "r") as f: + versions_from_ch_versions = yaml.safe_load(f) + + data_from_tools = { + "PARAMS_DICT": params_dict, + "PARAMS_TABLE": params_table, + "TOOLS_DICT": tools_dict, + "TOOLS_TABLE": tools_table, + "VERSIONS": { + **versions_from_ch_versions, + "JUICEBOX_JS": "2.4.3", + }, + **data_from_tools, + } + + report_printer = ReportPrinter() + report_template = report_printer.print_template(data_from_tools) + + with open("report.json", "w") as fp: + json.dump(data_from_tools, fp) diff --git a/bin/report_modules/parsers/assemblathon_stats_parser.py b/bin/report_modules/parsers/assemblathon_stats_parser.py new file mode 100644 index 00000000..8f124bf7 --- /dev/null +++ b/bin/report_modules/parsers/assemblathon_stats_parser.py @@ -0,0 +1,53 @@ +import os +from pathlib import Path +import pandas as pd +from tabulate import tabulate +import re + +from report_modules.parsers.parsing_commons import sort_list_of_results + + +def parse_assemblathon_stats_folder(folder_name="assemblathon_stats"): + dir = os.getcwdb().decode() + reports_folder_path = Path(f"{dir}/{folder_name}") + + if not os.path.exists(reports_folder_path): + return {} + + list_of_report_files = reports_folder_path.glob("*.csv") + + data = {"ASSEMBLATHON_STATS": []} + + for report_path in list_of_report_files: + report_table = pd.read_csv(report_path) + report_table.drop( + list(report_table.filter(regex="^Unnamed:")), axis=1, inplace=True + ) + + stat_names = report_table.columns.values.tolist() + stat_values = report_table.iloc[0].values.tolist() + + report_table_t = pd.DataFrame({"Stat": stat_names, "Value": stat_values}) + + file_tokens = re.findall( + r"([\w]+)_stats.csv", + os.path.basename(str(report_path)), + )[0] + + data["ASSEMBLATHON_STATS"].append( + { + "hap": file_tokens, + "report_table": report_table.to_dict("records"), + "report_table_html": tabulate( + report_table_t, + headers=["Stat", "Value"], + tablefmt="html", + numalign="left", + showindex=False, + ), + } + ) + + return { + "ASSEMBLATHON_STATS": sort_list_of_results(data["ASSEMBLATHON_STATS"], "hap") + } diff --git a/bin/report_modules/parsers/busco_parser.py b/bin/report_modules/parsers/busco_parser.py new file mode 100644 index 00000000..22dcff27 --- /dev/null +++ b/bin/report_modules/parsers/busco_parser.py @@ -0,0 +1,197 @@ +import pandas as pd +from tabulate import tabulate +import re +import os +import re +import base64 +from pathlib import Path + +from report_modules.parsers.parsing_commons import sort_list_of_results + + +class BuscoParser: + def __init__(self, file_data): + self.file_data = file_data + self.stats_dict = {} + + def parse_report(self): + self.stats_dict["version"] = self.get_busco_version(self.file_data) + self.stats_dict["lineage"] = self.get_lineage_dataset(self.file_data) + self.stats_dict["created"] = self.get_creation_date(self.file_data) + self.stats_dict["mode"] = self.get_run_mode(self.file_data) + self.stats_dict["predictor"] = self.get_gene_predictor(self.file_data) + self.stats_dict["search_percentages"] = self.get_busco_percentages( + self.file_data + ) + self.stats_dict["dependencies"] = self.get_deps_and_versions(self.file_data) + self.stats_dict["results_table"] = self.get_busco_result_table(self.file_data) + + # include busco results dictionary for use in json dump + self.stats_dict["results_dict"] = self.get_busco_result_dict(self.file_data) + # include dependencies dictionary for use in json dump + self.stats_dict["dependencies_dict"] = self.get_deps_and_versions_dict( + self.file_data + ) + + return self.stats_dict + + def get_busco_version(self, data): + p = re.compile("BUSCO version is: (.*)") + result = p.search(data).group(1).strip() + return result + + def get_lineage_dataset(self, data): + p = re.compile("The lineage dataset is: (.*)") + result = p.search(data).group(1).split()[0] + return result + + def get_creation_date(self, data): + p = re.compile("The lineage dataset is: (.*)") + result = p.search(data) + result = result.group(1).split()[3][:-1] + return result + + def get_run_mode(self, data): + p = re.compile("BUSCO was run in mode: (.*)") + result = p.search(data).group(1) + return result + + def get_gene_predictor(self, data): + p = re.compile("Gene predictor used: (.*)") + gene_predictor = p.search(data) + + if gene_predictor == None: + return "None" + + result = gene_predictor.group(1) + q = re.compile(f"{gene_predictor.group(1)}: (.*)") + predictor_version = q.search(data) + return result + + def get_busco_percentages(self, data): + p = re.compile("C:(.*)") + result = p.search(data).group(0).strip() + return result + + def get_deps_and_versions(self, file_data): + list_of_lines = file_data.split("\n") + for index, line in enumerate(list_of_lines): + if "Dependencies and versions" in line: + all_deps = ( + "".join(list_of_lines[max(0, index + 1) : len(list_of_lines) - 2]) + .replace("\t", "\n") + .strip() + ) + + dep_dict = {} + for dep in all_deps.splitlines(): + dependency = dep.split(":")[0] + version = dep.split(":")[1].strip() + dep_dict[f"{dependency}"] = f"{version}" + df = pd.DataFrame(dep_dict.items(), columns=["Dependency", "Version"]) + + col_names = ["Dependency", "Version"] + table = tabulate( + df, headers=col_names, tablefmt="html", numalign="left", showindex=False + ) + return table + + # get dependencies dictionary instead of table to use in json dump + def get_deps_and_versions_dict(self, file_data): + list_of_lines = file_data.split("\n") + for index, line in enumerate(list_of_lines): + if "Dependencies and versions" in line: + all_deps = ( + "".join(list_of_lines[max(0, index + 1) : len(list_of_lines) - 2]) + .replace("\t", "\n") + .strip() + ) + + dep_dict = {} + for dep in all_deps.splitlines(): + dependency = dep.split(":")[0] + version = dep.split(":")[1].strip() + dep_dict[f"{dependency}"] = f"{version}" + + return dep_dict + + def get_busco_result_table(self, file_data): + list_of_lines = file_data.split("\n") + for index, line in enumerate(list_of_lines): + if "Dependencies and versions" in line: + dev_dep_index = index + + results_dict = {} + for index, line in enumerate(list_of_lines): + if "C:" in line: + for i in range(index + 1, dev_dep_index - 1): + number = list_of_lines[i].split("\t")[1] + descr = list_of_lines[i].split("\t")[2] + + results_dict[f"{descr}"] = f"{number}" + df = pd.DataFrame(results_dict.items(), columns=["Event", "Frequency"]) + col_names = ["Event", "Frequency"] + table = tabulate( + df, headers=col_names, tablefmt="html", numalign="left", showindex=False + ) + return table + + # get results dictionary instead of table to use in json dump + def get_busco_result_dict(self, file_data): + list_of_lines = file_data.split("\n") + for index, line in enumerate(list_of_lines): + if "Dependencies and versions" in line: + dev_dep_index = index + + results_dict = {} + for index, line in enumerate(list_of_lines): + if "C:" in line: + for i in range(index + 1, dev_dep_index - 1): + number = list_of_lines[i].split("\t")[1] + descr = list_of_lines[i].split("\t")[2] + + results_dict[f"{descr}"] = f"{number}" + + return results_dict + + +def parse_busco_folder(folder_name="busco_outputs"): + dir = os.getcwdb().decode() + busco_folder_path = Path(f"{dir}/{folder_name}") + + if not os.path.exists(busco_folder_path): + return {} + + list_of_files = busco_folder_path.glob("*.txt") + + plot_path = next(busco_folder_path.glob("*.png")) + + binary_fc = open(plot_path, "rb").read() + base64_utf8_str = base64.b64encode(binary_fc).decode("utf-8") + ext = str(plot_path).split(".")[-1] + busco_plot_url = f"data:image/{ext};base64,{base64_utf8_str}" + + data = {"BUSCO": []} + + for file in list_of_files: + file_data = "" + with open(file, "r") as file: + lines = file.readlines() + for line in lines: + file_data += line + parser = BuscoParser(file_data) + file_tokens = re.findall( + r"short_summary.specific.([\w]+).([\w]+)_([a-zA-Z0-9]+).txt", + os.path.basename(str(file)), + )[0] + stats = { + "hap": file_tokens[1], + "lineage": file_tokens[0], + **parser.parse_report(), + } + data["BUSCO"].append(stats) + + data["BUSCO"] = sort_list_of_results(data["BUSCO"], "hap") + data["BUSCO"][0]["busco_plot"] = busco_plot_url + + return data diff --git a/bin/report_modules/parsers/circos_parser.py b/bin/report_modules/parsers/circos_parser.py new file mode 100644 index 00000000..0df2d037 --- /dev/null +++ b/bin/report_modules/parsers/circos_parser.py @@ -0,0 +1,52 @@ +import os +from pathlib import Path +import base64 +import re + +from report_modules.parsers.parsing_commons import sort_list_of_results + + +def parse_circos_folder(folder_name="circos_outputs"): + dir = os.getcwdb().decode() + circos_folder_path = Path(f"{dir}/{folder_name}") + + if not os.path.exists(circos_folder_path): + return {} + + list_of_plot_files = [item for item in circos_folder_path.glob("*.png")] + + data = {"CIRCOS": []} + + for plot_path in list_of_plot_files: + file_tokens = re.findall( + r"([\w]+).on.([\w]+).([\w]+).png", + os.path.basename(str(plot_path)), + )[0] + + if os.path.getsize(plot_path) == 0: + data["CIRCOS"].append( + { + "tag.on.tag": f"{file_tokens[0]} : {file_tokens[1]} : {file_tokens[2]}", + "circos_plot": "", + "is_plot_empty": True, + } + ) + continue + + binary_fc = open(plot_path, "rb").read() + base64_utf8_str = base64.b64encode(binary_fc).decode("utf-8") + ext = str(plot_path).split(".")[-1] + plot_url = f"data:image/{ext}+xml;base64,{base64_utf8_str}" + + data["CIRCOS"].append( + { + "tag.on.tag": f"{file_tokens[0]} : {file_tokens[1]} : {file_tokens[2]}", + "circos_plot": plot_url, + "is_plot_empty": False, + } + ) + + if len(data["CIRCOS"]) < 1: + return {} + + return {"CIRCOS": sort_list_of_results(data["CIRCOS"], "tag.on.tag")} diff --git a/bin/report_modules/parsers/fasta_validate_parser.py b/bin/report_modules/parsers/fasta_validate_parser.py new file mode 100644 index 00000000..786978ec --- /dev/null +++ b/bin/report_modules/parsers/fasta_validate_parser.py @@ -0,0 +1,35 @@ +import os +from pathlib import Path +import re + +from report_modules.parsers.parsing_commons import sort_list_of_results + + +def parse_fasta_validate_folder(folder_name="fastavalidator_logs"): + dir = os.getcwdb().decode() + logs_folder_path = Path(f"{dir}/{folder_name}") + + if not os.path.exists(logs_folder_path): + return {} + + list_of_log_files = logs_folder_path.glob("*.log") + + data = {"FASTA_VALIDATE": []} + + for log_path in list_of_log_files: + with open(log_path, "r") as f: + log_lines = [f"

    {l}

    " for l in f.readlines()] + + file_tokens = re.findall( + r"([\w]+).error.log", + os.path.basename(str(log_path)), + )[0] + + data["FASTA_VALIDATE"].append( + { + "hap": file_tokens, + "validation_log": "".join(log_lines), + } + ) + + return {"FASTA_VALIDATE": sort_list_of_results(data["FASTA_VALIDATE"], "hap")} diff --git a/bin/report_modules/parsers/genometools_gt_stat_parser.py b/bin/report_modules/parsers/genometools_gt_stat_parser.py new file mode 100644 index 00000000..a4e4e6f4 --- /dev/null +++ b/bin/report_modules/parsers/genometools_gt_stat_parser.py @@ -0,0 +1,344 @@ +import os +from pathlib import Path +import pandas as pd +from tabulate import tabulate +import re +import matplotlib.pyplot as plt +import numpy as np +import base64 + +from report_modules.parsers.parsing_commons import sort_list_of_results + + +def parse_genometools_gt_stat_folder(folder_name="genometools_gt_stat"): + dir = os.getcwdb().decode() + reports_folder_path = Path(f"{dir}/{folder_name}") + + if not os.path.exists(reports_folder_path): + return {} + + list_of_report_files = reports_folder_path.glob("*.yml") + + data = {"GENOMETOOLS_GT_STAT": []} + + for report_path in list_of_report_files: + + NUM_GROUPS = -1 + ( + report_table_dict, + gene_length_distribution, + gene_score_distribution, + exon_length_distribution, + exon_number_distribution, + intron_length_distribution, + cds_length_distribution, + ) = extract_report_data(report_path, NUM_GROUPS) + + gene_length_distribution_graph = "" + if gene_length_distribution != []: + gene_length_distribution_graph = create_dist_graph( + gene_length_distribution, + "Length", + "Gene Length Distribution", + f"./{folder_name}/{os.path.basename(report_path)}.gene.length.png", + ) + + gene_score_distribution_graph = "" + if gene_score_distribution != []: + gene_score_distribution_graph = create_dist_graph( + gene_score_distribution, + "Score", + "Gene Score Distribution", + f"./{folder_name}/{os.path.basename(report_path)}.gene.score.png", + ) + + exon_length_distribution_graph = "" + if exon_length_distribution != []: + exon_length_distribution_graph = create_dist_graph( + exon_length_distribution, + "Length", + "Exon Length Distribution", + f"./{folder_name}/{os.path.basename(report_path)}.exon.length.png", + ) + + exon_number_distribution_graph = "" + if exon_number_distribution != []: + exon_number_distribution_graph = create_dist_graph( + exon_number_distribution, + "Number", + "Exon Number Distribution", + f"./{folder_name}/{os.path.basename(report_path)}.exon.number.png", + ) + + intron_length_distribution_graph = "" + if intron_length_distribution != []: + intron_length_distribution_graph = create_dist_graph( + intron_length_distribution, + "Length", + "Intron Length Distribution", + f"./{folder_name}/{os.path.basename(report_path)}.intron.length.png", + ) + + cds_length_distribution_graph = "" + if cds_length_distribution != []: + cds_length_distribution_graph = create_dist_graph( + intron_length_distribution, + "Length", + "CDS Length Distribution", + f"./{folder_name}/{os.path.basename(report_path)}.cds.length.png", + ) + + file_tag = re.findall( + r"([\w]+).gt.stat.yml", + os.path.basename(str(report_path)), + )[0] + + data["GENOMETOOLS_GT_STAT"].append( + { + "hap": file_tag, + "report_table": report_table_dict, + "report_table_html": tabulate( + pd.DataFrame( + report_table_dict.items(), columns=["Metric", "Value"] + ), + headers=["Stat", "Value"], + tablefmt="html", + numalign="left", + showindex=False, + ), + "gene_length_plot": gene_length_distribution_graph, + "gene_score_plot": gene_score_distribution_graph, + "exon_length_plot": exon_length_distribution_graph, + "exon_number_plot": exon_number_distribution_graph, + "intron_length_plot": intron_length_distribution_graph, + "cds_length_plot": cds_length_distribution_graph, + } + ) + + return { + "GENOMETOOLS_GT_STAT": sort_list_of_results(data["GENOMETOOLS_GT_STAT"], "hap") + } + + +def extract_report_data(report_path, num_groups): + yaml_data = {} + parent_key = "" + with open(report_path, "r") as stream: + for line in stream: + key, value = line.strip().split(":", 1) + + if value == "": + parent_key = key + yaml_data[parent_key] = {} + continue + + if parent_key == "": + yaml_data[key] = value.strip() + continue + + yaml_data[parent_key][key] = value.strip() + + report_table_dict = { + key: value for key, value in yaml_data.items() if not isinstance(value, dict) + } + gene_length_distribution = create_frequency_groups( + [ + (int(key), int(value.split("(")[0].strip())) + for key, value in yaml_data["gene length distribution"].items() + ], + num_groups, + ) + gene_score_distribution = create_frequency_groups( + [ + (int(key), int(value.split("(")[0].strip())) + for key, value in yaml_data["gene score distribution"].items() + ], + num_groups, + ) + exon_length_distribution = create_frequency_groups( + [ + (int(key), int(value.split("(")[0].strip())) + for key, value in yaml_data["exon length distribution"].items() + ], + num_groups, + ) + exon_number_distribution = create_frequency_groups( + [ + (int(key), int(value.split("(")[0].strip())) + for key, value in yaml_data["exon number distribution"].items() + ], + num_groups, + ) + intron_length_distribution = create_frequency_groups( + [ + (int(key), int(value.split("(")[0].strip())) + for key, value in yaml_data["intron length distribution"].items() + ], + num_groups, + ) + cds_length_distribution = create_frequency_groups( + [ + (int(key), int(value.split("(")[0].strip())) + for key, value in yaml_data["CDS length distribution"].items() + ], + num_groups, + ) + + return ( + report_table_dict, + gene_length_distribution, + gene_score_distribution, + exon_length_distribution, + exon_number_distribution, + intron_length_distribution, + cds_length_distribution, + ) + + +def create_frequency_groups(data, num_groups): + + if num_groups == -1: + sorted_data = sorted(data, key=lambda x: x[0]) + return [ + { + "start": x, + "stop": x, + "freq": freq, + } + for x, freq in sorted_data + ] + + assert ( + num_groups >= 1 + ), f"num_groups should be larger than or equal to 1. It is {num_groups}" + + if data == []: + return [] + + sorted_data = sorted(data, key=lambda x: x[0]) + + ordinal = [x for x, _ in sorted_data] + + ordinal_max = max(ordinal) + ordinal_range = ordinal_max - min(ordinal) + ordinal_step = ordinal_range // num_groups + + groups = [] + current_group = { + "start": sorted_data[0][0], + "stop": [x for x in ordinal if x <= (sorted_data[0][0] + ordinal_step)][-1], + "freq": 0, + } + + for num, freq in sorted_data: + if num <= current_group["stop"]: + current_group["freq"] += freq + continue + + groups.append(current_group.copy()) + + current_group["start"] = num + current_group["stop"] = [x for x in ordinal if x <= (num + ordinal_step)][-1] + current_group["freq"] = freq + + groups.append(current_group) + + return groups + + +def test_create_frequency_groups_multiple(): + data = [(15, 4), (5, 1), (70, 10)] + num_groups = 2 + + expect = [ + {"start": 5, "stop": 15, "freq": 5}, + {"start": 70, "stop": 70, "freq": 10}, + ] + + assert expect == create_frequency_groups(data, num_groups) + + +def test_create_frequency_groups_single(): + data = [(15, 4)] + num_groups = 2 + + expect = [{"start": 15, "stop": 15, "freq": 4}] + + assert expect == create_frequency_groups(data, num_groups) + + +def test_create_frequency_groups_repeat(): + data = [(15, 4), (15, 8)] + num_groups = 2 + + expect = [{"start": 15, "stop": 15, "freq": 12}] + + assert expect == create_frequency_groups(data, num_groups) + + +# test_create_frequency_groups_multiple() +# test_create_frequency_groups_single() +# test_create_frequency_groups_repeat() + + +def create_dist_graph(groups_dict, x_label, title, file_name): + + x_list = [i["stop"] for i in groups_dict] + y_list = [i["freq"] for i in groups_dict] + sum_y = float(sum(y_list)) + cum_sum_y = np.cumsum(y_list) + y_list = [float(y) / sum_y * 100.0 for y in cum_sum_y] + + _, ax = plt.subplots() + ax.plot(x_list, y_list) + + ax.set_xlabel(x_label) + ax.set_ylabel("Cumulative percentage (%)") + ax.set_title(title) + + plt.gca().spines["top"].set_visible(False) + plt.gca().spines["right"].set_visible(False) + + min_x, min_y = (min(x_list), min(y_list)) + x_anno_step = int(float(max(x_list)) * 0.1) + ax.annotate( + f"(<={min_x}, {round(min_y, 2)}%)", + xy=(min_x, min_y), + xytext=(min_x + x_anno_step, min_y + 10), + arrowprops=dict(color="red", arrowstyle="->, head_width=.15"), + ) + + near_50 = min([y for y in y_list if y >= 50.0]) + min_x, min_y = (x_list[y_list.index(near_50)], near_50) + ax.annotate( + f"(<={min_x}, {round(min_y, 2)}%)", + xy=(min_x, min_y), + xytext=(min_x + x_anno_step, min_y), + arrowprops=dict(color="red", arrowstyle="->, head_width=.15"), + ) + + near_90 = min([y for y in y_list if y >= 90.0]) + min_x, min_y = (x_list[y_list.index(near_90)], near_90) + ax.annotate( + f"(<={min_x}, {round(min_y, 2)}%)", + xy=(min_x, min_y), + xytext=(min_x + x_anno_step, min_y - 10), + arrowprops=dict(color="red", arrowstyle="->, head_width=.15"), + ) + + near_3_sigma = min([y for y in y_list if y >= 99.7]) + min_x, min_y = (x_list[y_list.index(near_3_sigma)], near_3_sigma) + ax.annotate( + f"(<={min_x}, {round(min_y, 2)}%)", + xy=(min_x, min_y), + xytext=(min_x + x_anno_step, min_y - 10), + arrowprops=dict(color="red", arrowstyle="->, head_width=.15"), + ) + + plt.savefig(file_name, dpi=600) + + with open(file_name, "rb") as f: + binary_fc = f.read() + + base64_utf8_str = base64.b64encode(binary_fc).decode("utf-8") + return f"data:image/png+xml;base64,{base64_utf8_str}" diff --git a/bin/report_modules/parsers/gff3_validate_parser.py b/bin/report_modules/parsers/gff3_validate_parser.py new file mode 100644 index 00000000..e9a7d1e7 --- /dev/null +++ b/bin/report_modules/parsers/gff3_validate_parser.py @@ -0,0 +1,35 @@ +import os +from pathlib import Path +import re + +from report_modules.parsers.parsing_commons import sort_list_of_results + + +def parse_gff3_validate_folder(folder_name="gff3_validate_logs"): + dir = os.getcwdb().decode() + logs_folder_path = Path(f"{dir}/{folder_name}") + + if not os.path.exists(logs_folder_path): + return {} + + list_of_log_files = logs_folder_path.glob("*.log") + + data = {"GFF3_VALIDATE": []} + + for log_path in list_of_log_files: + with open(log_path, "r") as f: + log_lines = [f"

    {l}

    " for l in f.readlines()] + + file_tokens = re.findall( + r"([\w]+).error.log", + os.path.basename(str(log_path)), + )[0] + + data["GFF3_VALIDATE"].append( + { + "hap": file_tokens, + "validation_log": "".join(log_lines), + } + ) + + return {"GFF3_VALIDATE": sort_list_of_results(data["GFF3_VALIDATE"], "hap")} diff --git a/bin/report_modules/parsers/hic_parser.py b/bin/report_modules/parsers/hic_parser.py new file mode 100644 index 00000000..825852ec --- /dev/null +++ b/bin/report_modules/parsers/hic_parser.py @@ -0,0 +1,34 @@ +import os +from pathlib import Path +import re + +from report_modules.parsers.parsing_commons import sort_list_of_results + + +def parse_hic_folder(folder_name="hic_outputs"): + dir = os.getcwdb().decode() + hic_folder_path = Path(f"{dir}/{folder_name}") + + if not os.path.exists(hic_folder_path): + return {} + + list_of_hic_files = hic_folder_path.glob("*.html") + + data = {"HIC": []} + + for hic_path in list_of_hic_files: + hic_file_name = os.path.basename(str(hic_path)) + + file_tokens = re.findall( + r"([\w]+).html", + hic_file_name, + )[0] + + data["HIC"].append( + { + "hap": file_tokens, + "hic_html_file_name": hic_file_name, + } + ) + + return {"HIC": sort_list_of_results(data["HIC"], "hap")} diff --git a/bin/report_modules/parsers/kraken2_parser.py b/bin/report_modules/parsers/kraken2_parser.py new file mode 100644 index 00000000..f10fae13 --- /dev/null +++ b/bin/report_modules/parsers/kraken2_parser.py @@ -0,0 +1,34 @@ +import os +from pathlib import Path +import re + +from report_modules.parsers.parsing_commons import sort_list_of_results + + +def parse_kraken2_folder(folder_name="kraken2_outputs"): + dir = os.getcwdb().decode() + kraken2_folder_path = Path(f"{dir}/{folder_name}") + + if not os.path.exists(kraken2_folder_path): + return {} + + list_of_html_files = kraken2_folder_path.glob("*.html") + + data = {"KRAKEN2": []} + + for html_path in list_of_html_files: + html_file_name = os.path.basename(str(html_path)) + + file_tokens = re.findall( + r"([\w]+).kraken2.krona.html", + html_file_name, + )[0] + + data["KRAKEN2"].append( + { + "hap": file_tokens, + "krona_html_file_name": html_file_name, + } + ) + + return {"KRAKEN2": sort_list_of_results(data["KRAKEN2"], "hap")} diff --git a/bin/report_modules/parsers/lai_parser.py b/bin/report_modules/parsers/lai_parser.py new file mode 100644 index 00000000..7318e92a --- /dev/null +++ b/bin/report_modules/parsers/lai_parser.py @@ -0,0 +1,95 @@ +import os +from pathlib import Path +import re + +from report_modules.parsers.parsing_commons import sort_list_of_results + + +class LAIParser: + def __init__(self, log_file_data, out_file_data): + self.log_file_data = log_file_data + self.out_file_data = out_file_data + self.stats_dict = {} + + def parse_report(self): + self.stats_dict["version"] = self.get_lai_version() + lai_errors = self.get_lai_errors() + + if lai_errors != None: + self.stats_dict["result"] = lai_errors + return self.stats_dict + + lai_stats = self.get_lai_stats() + + self.stats_dict["result"] = lai_stats + return self.stats_dict + + def get_lai_version(self): + p = re.compile("### LTR Assembly Index \(LAI\) (.*) ###") + result = p.search(self.log_file_data).group(1).strip() + return result + + def get_lai_errors(self): + p = re.compile("【Error】(.*)") + match_results = p.findall(self.log_file_data) + if len(match_results) < 1: + return None + + return ". ".join([m.strip() for m in match_results]) + + def get_lai_stats(self): + p = re.compile(r"whole_genome(.*)") + match_results = p.findall(self.out_file_data) + if len(match_results) != 1: + return "Error parsing the LAI.out file" + + raw_stats = match_results[0].strip().split("\t") + + if len(raw_stats) != 6: + return "Error parsing the LAI.out file" + + stats_str = f"Intact: {raw_stats[2]}, Total: {raw_stats[3]}, Raw LAI: {raw_stats[4]}, LAI: {raw_stats[5]}" + + return stats_str + + +def parse_lai_folder(folder_name="lai_outputs"): + dir = os.getcwdb().decode() + lai_folder_path = Path(f"{dir}/{folder_name}") + + if not os.path.exists(lai_folder_path): + return {} + + list_of_log_files = lai_folder_path.glob("*.LAI.log") + + data = {"LAI": []} + + for file in list_of_log_files: + log_file_data = "" + with open(file, "r") as file: + lines = file.readlines() + for line in lines: + log_file_data += line + + file_tokens = re.findall( + r"([\w]+).LAI.log", + os.path.basename(str(file)), + ) + + hap_name = file_tokens[0] + out_file_path = Path(f"{dir}/{folder_name}/{hap_name}.LAI.out") + out_file_data = "" + if os.path.exists(out_file_path): + with open(out_file_path, "r") as out_file: + lines = out_file.readlines() + for line in lines: + out_file_data += line + + parser = LAIParser(log_file_data, out_file_data) + stats = { + "hap": hap_name, + **parser.parse_report(), + } + data["LAI"].append(stats) + + return {"LAI": sort_list_of_results(data["LAI"], "hap")} diff --git a/bin/report_modules/parsers/ncbi_fcs_adaptor_parser.py b/bin/report_modules/parsers/ncbi_fcs_adaptor_parser.py new file mode 100644 index 00000000..f896577f --- /dev/null +++ b/bin/report_modules/parsers/ncbi_fcs_adaptor_parser.py @@ -0,0 +1,44 @@ +import os +from pathlib import Path +import pandas as pd +from tabulate import tabulate +import re + +from report_modules.parsers.parsing_commons import sort_list_of_results + + +def parse_ncbi_fcs_adaptor_folder(folder_name="ncbi_fcs_adaptor_reports"): + dir = os.getcwdb().decode() + reports_folder_path = Path(f"{dir}/{folder_name}") + + if not os.path.exists(reports_folder_path): + return {} + + list_of_report_files = reports_folder_path.glob("*.tsv") + + data = {"NCBI_FCS_ADAPTOR": []} + + for report_path in list_of_report_files: + report_table = pd.read_csv(report_path, sep="\t") + + file_tokens = re.findall( + r"([\w]+)_fcs_adaptor_report.tsv", + os.path.basename(str(report_path)), + )[0] + + data["NCBI_FCS_ADAPTOR"].append( + { + "hap": file_tokens, + "did_detect_contamination": report_table.shape[0] > 0, + "report_table": report_table.to_dict("records"), + "report_table_html": tabulate( + report_table, + headers=["Accession No.", "Length", "Action", "Range", "Name"], + tablefmt="html", + numalign="left", + showindex=False, + ), + } + ) + + return {"NCBI_FCS_ADAPTOR": sort_list_of_results(data["NCBI_FCS_ADAPTOR"], "hap")} diff --git a/bin/report_modules/parsers/ncbi_fcs_gx_parser.py b/bin/report_modules/parsers/ncbi_fcs_gx_parser.py new file mode 100644 index 00000000..8ee14e21 --- /dev/null +++ b/bin/report_modules/parsers/ncbi_fcs_gx_parser.py @@ -0,0 +1,56 @@ +import os +from pathlib import Path +import pandas as pd +from tabulate import tabulate +import re +import json + +from report_modules.parsers.parsing_commons import sort_list_of_results + + +def parse_ncbi_fcs_gx_folder(folder_name="fcs_gx_reports"): + dir = os.getcwdb().decode() + reports_folder_path = Path(f"{dir}/{folder_name}") + + if not os.path.exists(reports_folder_path): + return {} + + list_of_report_files = reports_folder_path.glob("*.txt") + + data = {"NCBI_FCS_GX": []} + + for report_path in list_of_report_files: + with open(report_path, "r") as f: + meta_data = json.loads(f.readline()[2:-1]) + + asserted_div = meta_data[1]["run-info"]["asserted-div"] + inferred_primary_divs = meta_data[1]["run-info"]["inferred-primary-divs"] + + report_table = pd.read_csv(report_path, sep="\t", skiprows=1) + + file_tokens = re.findall( + r"([\w]+).fcs_gx_report.txt", + os.path.basename(str(report_path)), + )[0] + + data["NCBI_FCS_GX"].append( + { + "hap": file_tokens, + "did_detect_contamination": report_table.shape[0] > 0, + "report_table": report_table.to_dict("records"), + "report_table_html": tabulate( + report_table.iloc[:, [0, 1, 2, 3, 4, 7]], + headers=["Seq ID", "Start", "End", "Length", "Action", "Tax name"], + tablefmt="html", + numalign="left", + showindex=False, + ), + "report_meta_data": meta_data, + "is_wrong_div": False + if asserted_div in inferred_primary_divs + else True, + "krona_html_file_name": f"{file_tokens}.fcs.gx.krona.html", + } + ) + + return {"NCBI_FCS_GX": sort_list_of_results(data["NCBI_FCS_GX"], "hap")} diff --git a/bin/report_modules/parsers/params_parser.py b/bin/report_modules/parsers/params_parser.py new file mode 100644 index 00000000..bf07dc5a --- /dev/null +++ b/bin/report_modules/parsers/params_parser.py @@ -0,0 +1,43 @@ +import json + +from pygments import highlight +from pygments.lexers import JsonLexer +from pygments.formatters import HtmlFormatter + + +def highlight_json(json_string): + lexer = JsonLexer() + formatter = HtmlFormatter() + + return highlight(json_string, lexer, formatter) + + +def format_params_dict(json_dict): + formatted_dict = {} + for key, value in json_dict.items(): + if key in ["max_cpus", "max_memory", "max_time"]: + continue + + if not isinstance(value, dict): + formatted_dict[key] = value + continue + + if "skip" in value.keys(): + if value["skip"] == 1: + formatted_dict[key] = "Skipped" + continue + + formatted_dict[key] = value + formatted_dict[key].pop("skip", None) + + return formatted_dict + + +def parse_params_json(): + with open("params_json.json", "r") as f: + params_dict = json.load(f) + formatted_dict_json = highlight_json( + json.dumps(format_params_dict(params_dict), indent=4) + ) + + return params_dict, formatted_dict_json diff --git a/bin/report_modules/parsers/parsing_commons.py b/bin/report_modules/parsers/parsing_commons.py new file mode 100644 index 00000000..ff46c481 --- /dev/null +++ b/bin/report_modules/parsers/parsing_commons.py @@ -0,0 +1,10 @@ +import re + + +def natural_key(string): + """Return a list of keys that sort naturally.""" + return [int(s) if s.isdigit() else s for s in re.split(r"(\d+)", string)] + + +def sort_list_of_results(results_list, on_key): + return sorted(results_list, key=lambda x: natural_key(x[on_key])) diff --git a/bin/report_modules/parsers/tidk_parser.py b/bin/report_modules/parsers/tidk_parser.py new file mode 100644 index 00000000..556d93a4 --- /dev/null +++ b/bin/report_modules/parsers/tidk_parser.py @@ -0,0 +1,68 @@ +import os +from pathlib import Path +import base64 +import re + +from report_modules.parsers.parsing_commons import sort_list_of_results + + +def parse_tidk_folder(folder_name="tidk_outputs"): + dir = os.getcwdb().decode() + tidk_folder_path = Path(f"{dir}/{folder_name}") + + if not os.path.exists(tidk_folder_path): + return {} + + list_of_plot_files = tidk_folder_path.glob("*.svg") + + data = {"TIDK": []} + + # get the a_prior_sequence file + a_priori_sequence_file_name = "a_priori.sequence" + with open(f"{dir}/{folder_name}/{a_priori_sequence_file_name}", "r") as file: + lines = file.readlines() + a_priori_sequence = lines[0].strip() + + for plot_path in list_of_plot_files: + binary_fc = open(plot_path, "rb").read() + base64_utf8_str = base64.b64encode(binary_fc).decode("utf-8") + ext = str(plot_path).split(".")[-1] + plot_url = f"data:image/{ext}+xml;base64,{base64_utf8_str}" + + file_tokens = re.findall( + r"([\w]+).([\w]+).svg", + os.path.basename(str(plot_path)), + )[0] + + sample_tag = file_tokens[0].strip() + plot_type = file_tokens[1].strip() + if "aposteriori" in plot_type: + sequence_file_name = f"{sample_tag}.top.sequence.txt" + + with open(f"{dir}/{folder_name}/{sequence_file_name}", "r") as file: + lines = file.readlines() + sequence = "" if len(lines) < 1 else lines[0].strip() + + display_name = f"{sample_tag}: a posteriori sequence" + + else: + display_name = f"{sample_tag}: a priori sequence" + sequence = "" + + data["TIDK"].append( + { + "hap": f"{sample_tag}_{plot_type}", + "hap_display": display_name, + "sequence": sequence, + "is_a_priori": "a priori" in display_name, + "a_priori_sequence": a_priori_sequence, + "has_sequence": sequence != "", + "tidk_plot": plot_url, + "tidk_plot_empty": False, + } + ) + + if len(data["TIDK"]) < 1: + return {} + + return {"TIDK": sort_list_of_results(data["TIDK"], "hap")} diff --git a/bin/report_modules/parsers/tools_parser.py b/bin/report_modules/parsers/tools_parser.py new file mode 100644 index 00000000..54746094 --- /dev/null +++ b/bin/report_modules/parsers/tools_parser.py @@ -0,0 +1,33 @@ +import yaml +import json + +from pygments import highlight +from pygments.lexers import JsonLexer +from pygments.formatters import HtmlFormatter + + +def parse_tools_yaml(): + with open("software_versions.yml", "r") as f: + tools_dict = yaml.safe_load(f) + formatted_tools_json = highlight_json( + json.dumps(format_tools_dict(tools_dict), indent=4) + ) + + return tools_dict, formatted_tools_json + + +def highlight_json(json_string): + lexer = JsonLexer() + formatter = HtmlFormatter() + + return highlight(json_string, lexer, formatter) + + +def format_tools_dict(input_dict): + output_list = [] + for _, top_level_value in input_dict.items(): + for key, value in top_level_value.items(): + if (key, value) not in output_list: + output_list.append((key, value)) + + return dict(sorted(output_list, key=lambda x: x[0])) diff --git a/bin/report_modules/report_printer.py b/bin/report_modules/report_printer.py new file mode 100644 index 00000000..24e58f7a --- /dev/null +++ b/bin/report_modules/report_printer.py @@ -0,0 +1,15 @@ +from jinja2 import Environment, FileSystemLoader +from pathlib import Path + + +class ReportPrinter: + def __init__(self): + projectDir = "/".join(__file__.split("/")[0:-1]) + path = Path(f"{projectDir}/templates") + + self.file_loader = FileSystemLoader(path) + self.env = Environment(loader=self.file_loader) + + def print_template(self, stats): + template = self.env.get_template("base.html") + print(template.render(all_stats_dicts=stats)) diff --git a/bin/report_modules/templates/assemblathon_stats/assemblathon_stats.html b/bin/report_modules/templates/assemblathon_stats/assemblathon_stats.html new file mode 100644 index 00000000..d8e5b82d --- /dev/null +++ b/bin/report_modules/templates/assemblathon_stats/assemblathon_stats.html @@ -0,0 +1,21 @@ +     diff --git a/bin/report_modules/templates/assemblathon_stats/dropdown.html b/bin/report_modules/templates/assemblathon_stats/dropdown.html new file mode 100644 index 00000000..8be7bfa6 --- /dev/null +++ b/bin/report_modules/templates/assemblathon_stats/dropdown.html @@ -0,0 +1,10 @@ +
    +
    + +
    +
    diff --git a/bin/report_modules/templates/assemblathon_stats/report_contents.html b/bin/report_modules/templates/assemblathon_stats/report_contents.html new file mode 100644 index 00000000..34bf5f7e --- /dev/null +++ b/bin/report_modules/templates/assemblathon_stats/report_contents.html @@ -0,0 +1,17 @@ +{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["ASSEMBLATHON_STATS"]|length) %} {% set +active_text = 'display: block' if vars.is_first else 'display: none' %} +
    +
    +
    +
    {{ all_stats_dicts['ASSEMBLATHON_STATS'][item]['hap'] }}
    +
    +
    +
    +
    {{ all_stats_dicts['ASSEMBLATHON_STATS'][item]['report_table_html'] }}
    +
    +
    +{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/bin/report_modules/templates/base.html b/bin/report_modules/templates/base.html new file mode 100644 index 00000000..e0b8eef0 --- /dev/null +++ b/bin/report_modules/templates/base.html @@ -0,0 +1,68 @@ + + {% include 'header.html' %} + + +
    +
    AssemblyQC {{ all_stats_dicts['VERSIONS']['Workflow']['AssemblyQC'] }}
    +
    +
    + + + {% if 'GFF3_VALIDATE' in all_stats_dicts %} + + {% endif %} {% if 'FASTA_VALIDATE' in all_stats_dicts %} + + {% endif %} {% if 'NCBI_FCS_ADAPTOR' in all_stats_dicts %} + + {% endif %} {% if 'NCBI_FCS_GX' in all_stats_dicts %}{% endif %} {% if 'ASSEMBLATHON_STATS' in all_stats_dicts %}{% endif %} {% if 'GENOMETOOLS_GT_STAT' in all_stats_dicts %}{% endif %} {% if 'BUSCO' in all_stats_dicts %}{% endif %} {% if 'TIDK' in all_stats_dicts %}{% endif %} {% if 'LAI' in all_stats_dicts %}{% endif %} {% if 'KRAKEN2' in all_stats_dicts %}{% endif %} {% if 'HIC' in all_stats_dicts %}{% endif %} {% if 'CIRCOS' in all_stats_dicts %}{% endif %} +
    + {% include 'params/params.html' %} {% include 'tools/tools.html' %} {% if 'GFF3_VALIDATE' in all_stats_dicts + %}{% include 'gff3_validate/gff3_validate.html' %}{% endif %} {% if 'FASTA_VALIDATE' in all_stats_dicts %}{% + include 'fasta_validate/fasta_validate.html' %}{% endif %} {% if 'NCBI_FCS_ADAPTOR' in all_stats_dicts %}{% + include 'ncbi_fcs_adaptor/ncbi_fcs_adaptor.html' %}{% endif %} {% if 'NCBI_FCS_GX' in all_stats_dicts %}{% + include 'ncbi_fcs_gx/ncbi_fcs_gx.html' %}{% endif %} {% if 'ASSEMBLATHON_STATS' in all_stats_dicts %}{% include + 'assemblathon_stats/assemblathon_stats.html' %}{% endif %} {% if 'GENOMETOOLS_GT_STAT' in all_stats_dicts %}{% + include 'genometools_gt_stat/genometools_gt_stat.html' %}{% endif %} {% if 'BUSCO' in all_stats_dicts %}{% + include 'busco/busco.html' %}{% endif %} {% if 'TIDK' in all_stats_dicts %}{% include 'tidk/tidk.html' %}{% + endif %} {% if 'LAI' in all_stats_dicts %}{% include 'lai/lai.html' %}{% endif %} {% if 'KRAKEN2' in + all_stats_dicts %}{% include 'kraken2/kraken2.html' %}{% endif %} {% if 'HIC' in all_stats_dicts %}{% include + 'hic/hic.html' %}{% endif %} {% if 'CIRCOS' in all_stats_dicts %}{% include 'circos/circos.html' %}{% endif %} + + {% include 'js.html' %} + diff --git a/bin/report_modules/templates/busco/busco.html b/bin/report_modules/templates/busco/busco.html new file mode 100644 index 00000000..06d560e5 --- /dev/null +++ b/bin/report_modules/templates/busco/busco.html @@ -0,0 +1,18 @@ + diff --git a/bin/report_modules/templates/busco/dropdown.html b/bin/report_modules/templates/busco/dropdown.html new file mode 100644 index 00000000..23981b8c --- /dev/null +++ b/bin/report_modules/templates/busco/dropdown.html @@ -0,0 +1,13 @@ +
    +
    + +
    +
    diff --git a/bin/report_modules/templates/busco/report_contents.html b/bin/report_modules/templates/busco/report_contents.html new file mode 100644 index 00000000..1c552652 --- /dev/null +++ b/bin/report_modules/templates/busco/report_contents.html @@ -0,0 +1,77 @@ +{% for item in range(all_stats_dicts["BUSCO"]|length) %} + +{% endfor %} diff --git a/bin/report_modules/templates/busco/summary_contents.html b/bin/report_modules/templates/busco/summary_contents.html new file mode 100644 index 00000000..d289b4aa --- /dev/null +++ b/bin/report_modules/templates/busco/summary_contents.html @@ -0,0 +1,37 @@ +
    +
    +
    +
    Summary
    +
    + +
    +
    + + + + + + + + + + {% for item in range(all_stats_dicts["BUSCO"]|length) %} + + + + + + {% endfor %} + +
    AssemblyLineagePercentages
    + {{ all_stats_dicts['BUSCO'][item]['hap'] }} + + {{ all_stats_dicts['BUSCO'][item]['lineage'] }} + {{ all_stats_dicts['BUSCO'][item]['search_percentages'] }}
    +
    +
    +
    +
    + +
    +
    diff --git a/bin/report_modules/templates/circos/circos.html b/bin/report_modules/templates/circos/circos.html new file mode 100644 index 00000000..2099a251 --- /dev/null +++ b/bin/report_modules/templates/circos/circos.html @@ -0,0 +1,32 @@ + diff --git a/bin/report_modules/templates/circos/dropdown.html b/bin/report_modules/templates/circos/dropdown.html new file mode 100644 index 00000000..0cdafbb7 --- /dev/null +++ b/bin/report_modules/templates/circos/dropdown.html @@ -0,0 +1,10 @@ +
    +
    + +
    +
    diff --git a/bin/report_modules/templates/circos/report_contents.html b/bin/report_modules/templates/circos/report_contents.html new file mode 100644 index 00000000..e3355169 --- /dev/null +++ b/bin/report_modules/templates/circos/report_contents.html @@ -0,0 +1,26 @@ +{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["CIRCOS"]|length) %} {% set active_text = +'display: block' if vars.is_first else 'display: none' %} +
    +
    +
    +
    {{ all_stats_dicts['CIRCOS'][item]['tag.on.tag'] }}
    +
    + {% if all_stats_dicts['CIRCOS'][item]['is_plot_empty'] %} +
    +

    + Either no alignments were detected between the target and reference assemblies, or all the alignment + bundles were filtered out 😔 +

    +
    + {% else %} +
    + +
    + {% endif %} +
    +
    +{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/bin/report_modules/templates/fasta_validate/dropdown.html b/bin/report_modules/templates/fasta_validate/dropdown.html new file mode 100644 index 00000000..56fb72ef --- /dev/null +++ b/bin/report_modules/templates/fasta_validate/dropdown.html @@ -0,0 +1,10 @@ +
    +
    + +
    +
    diff --git a/bin/report_modules/templates/fasta_validate/fasta_validate.html b/bin/report_modules/templates/fasta_validate/fasta_validate.html new file mode 100644 index 00000000..e0643542 --- /dev/null +++ b/bin/report_modules/templates/fasta_validate/fasta_validate.html @@ -0,0 +1,14 @@ + diff --git a/bin/report_modules/templates/fasta_validate/report_contents.html b/bin/report_modules/templates/fasta_validate/report_contents.html new file mode 100644 index 00000000..7abaee35 --- /dev/null +++ b/bin/report_modules/templates/fasta_validate/report_contents.html @@ -0,0 +1,18 @@ +{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["FASTA_VALIDATE"]|length) %} {% set active_text += 'display: block' if vars.is_first else 'display: none' %} +
    +
    +
    +
    {{ all_stats_dicts['FASTA_VALIDATE'][item]['hap'] }}
    +
    +
    +

    Validation failed!

    +
    +
    {{ all_stats_dicts['FASTA_VALIDATE'][item]['validation_log'] }}
    +
    +
    +{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/bin/report_modules/templates/genometools_gt_stat/dropdown.html b/bin/report_modules/templates/genometools_gt_stat/dropdown.html new file mode 100644 index 00000000..cd948bdf --- /dev/null +++ b/bin/report_modules/templates/genometools_gt_stat/dropdown.html @@ -0,0 +1,10 @@ +
    +
    + +
    +
    diff --git a/bin/report_modules/templates/genometools_gt_stat/genometools_gt_stat.html b/bin/report_modules/templates/genometools_gt_stat/genometools_gt_stat.html new file mode 100644 index 00000000..c5a5e7a1 --- /dev/null +++ b/bin/report_modules/templates/genometools_gt_stat/genometools_gt_stat.html @@ -0,0 +1,15 @@ + diff --git a/bin/report_modules/templates/genometools_gt_stat/report_contents.html b/bin/report_modules/templates/genometools_gt_stat/report_contents.html new file mode 100644 index 00000000..d2975ede --- /dev/null +++ b/bin/report_modules/templates/genometools_gt_stat/report_contents.html @@ -0,0 +1,35 @@ +{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["GENOMETOOLS_GT_STAT"]|length) %} {% set +active_text = 'display: block' if vars.is_first else 'display: none' %} +
    +
    +
    +
    {{ all_stats_dicts['GENOMETOOLS_GT_STAT'][item]['hap'] }}
    +
    +
    +
    +
    {{ all_stats_dicts['GENOMETOOLS_GT_STAT'][item]['report_table_html'] }}
    +
    +
    + +
    +
    + +
    +
    + +
    +
    + +
    +
    + +
    +
    + +
    +
    +{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/bin/report_modules/templates/gff3_validate/dropdown.html b/bin/report_modules/templates/gff3_validate/dropdown.html new file mode 100644 index 00000000..0bece7a0 --- /dev/null +++ b/bin/report_modules/templates/gff3_validate/dropdown.html @@ -0,0 +1,10 @@ +
    +
    + +
    +
    diff --git a/bin/report_modules/templates/gff3_validate/gff3_validate.html b/bin/report_modules/templates/gff3_validate/gff3_validate.html new file mode 100644 index 00000000..01fa2754 --- /dev/null +++ b/bin/report_modules/templates/gff3_validate/gff3_validate.html @@ -0,0 +1,22 @@ + diff --git a/bin/report_modules/templates/gff3_validate/report_contents.html b/bin/report_modules/templates/gff3_validate/report_contents.html new file mode 100644 index 00000000..d61358a2 --- /dev/null +++ b/bin/report_modules/templates/gff3_validate/report_contents.html @@ -0,0 +1,18 @@ +{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["GFF3_VALIDATE"]|length) %} {% set active_text += 'display: block' if vars.is_first else 'display: none' %} +
    +
    +
    +
    {{ all_stats_dicts['GFF3_VALIDATE'][item]['hap'] }}
    +
    +
    +

    Validation failed!

    +
    +
    {{ all_stats_dicts['GFF3_VALIDATE'][item]['validation_log'] }}
    +
    +
    +{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/bin/report_modules/templates/header.html b/bin/report_modules/templates/header.html new file mode 100644 index 00000000..989b37f0 --- /dev/null +++ b/bin/report_modules/templates/header.html @@ -0,0 +1,637 @@ + + AssemblyQC + + + + + + + + diff --git a/bin/report_modules/templates/hic/dropdown.html b/bin/report_modules/templates/hic/dropdown.html new file mode 100644 index 00000000..4cd50f43 --- /dev/null +++ b/bin/report_modules/templates/hic/dropdown.html @@ -0,0 +1,10 @@ +
    +
    + +
    +
    diff --git a/bin/report_modules/templates/hic/hic.html b/bin/report_modules/templates/hic/hic.html new file mode 100644 index 00000000..868dc089 --- /dev/null +++ b/bin/report_modules/templates/hic/hic.html @@ -0,0 +1,18 @@ + diff --git a/bin/report_modules/templates/hic/report_contents.html b/bin/report_modules/templates/hic/report_contents.html new file mode 100644 index 00000000..312a0fdd --- /dev/null +++ b/bin/report_modules/templates/hic/report_contents.html @@ -0,0 +1,17 @@ +{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["HIC"]|length) %} {% set active_text = +'display: block' if vars.is_first else 'display: none' %} +
    +
    +
    +
    {{ all_stats_dicts['HIC'][item]['hap'] }}
    +
    +
    + +
    +
    +
    +{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/bin/report_modules/templates/js.html b/bin/report_modules/templates/js.html new file mode 100644 index 00000000..fd0f8d21 --- /dev/null +++ b/bin/report_modules/templates/js.html @@ -0,0 +1,58 @@ + diff --git a/bin/report_modules/templates/kraken2/dropdown.html b/bin/report_modules/templates/kraken2/dropdown.html new file mode 100644 index 00000000..cced9430 --- /dev/null +++ b/bin/report_modules/templates/kraken2/dropdown.html @@ -0,0 +1,11 @@ +
    +
    + +
    +
    diff --git a/bin/report_modules/templates/kraken2/kraken2.html b/bin/report_modules/templates/kraken2/kraken2.html new file mode 100644 index 00000000..1b87e9e2 --- /dev/null +++ b/bin/report_modules/templates/kraken2/kraken2.html @@ -0,0 +1,15 @@ + diff --git a/bin/report_modules/templates/kraken2/report_contents.html b/bin/report_modules/templates/kraken2/report_contents.html new file mode 100644 index 00000000..65f09183 --- /dev/null +++ b/bin/report_modules/templates/kraken2/report_contents.html @@ -0,0 +1,21 @@ +{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["KRAKEN2"]|length) %} {% set active_text = +'display: block' if vars.is_first else 'display: none' %} +
    +
    +
    +
    {{ all_stats_dicts['KRAKEN2'][item]['hap'] }}
    +
    +
    + +
    +
    +
    +{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/bin/report_modules/templates/lai/dropdown.html b/bin/report_modules/templates/lai/dropdown.html new file mode 100644 index 00000000..ca7e0c1b --- /dev/null +++ b/bin/report_modules/templates/lai/dropdown.html @@ -0,0 +1,7 @@ +
    +
    + +
    +
    diff --git a/bin/report_modules/templates/lai/lai.html b/bin/report_modules/templates/lai/lai.html new file mode 100644 index 00000000..4b0775ae --- /dev/null +++ b/bin/report_modules/templates/lai/lai.html @@ -0,0 +1,26 @@ + diff --git a/bin/report_modules/templates/lai/summary_contents.html b/bin/report_modules/templates/lai/summary_contents.html new file mode 100644 index 00000000..7844edb9 --- /dev/null +++ b/bin/report_modules/templates/lai/summary_contents.html @@ -0,0 +1,30 @@ +
    +
    +
    +
    Summary
    +
    + +
    +
    + + + + + + + + + {% for item in range(all_stats_dicts["LAI"]|length) %} + + + + + {% endfor %} + +
    AssemblyResults
    {{ all_stats_dicts['LAI'][item]['hap'] }} + {{ all_stats_dicts['LAI'][item]['result'] }} +
    +
    +
    +
    +
    diff --git a/bin/report_modules/templates/ncbi_fcs_adaptor/dropdown.html b/bin/report_modules/templates/ncbi_fcs_adaptor/dropdown.html new file mode 100644 index 00000000..9f21bc26 --- /dev/null +++ b/bin/report_modules/templates/ncbi_fcs_adaptor/dropdown.html @@ -0,0 +1,10 @@ +
    +
    + +
    +
    diff --git a/bin/report_modules/templates/ncbi_fcs_adaptor/ncbi_fcs_adaptor.html b/bin/report_modules/templates/ncbi_fcs_adaptor/ncbi_fcs_adaptor.html new file mode 100644 index 00000000..e62678c9 --- /dev/null +++ b/bin/report_modules/templates/ncbi_fcs_adaptor/ncbi_fcs_adaptor.html @@ -0,0 +1,11 @@ + diff --git a/bin/report_modules/templates/ncbi_fcs_adaptor/report_contents.html b/bin/report_modules/templates/ncbi_fcs_adaptor/report_contents.html new file mode 100644 index 00000000..ae1cd77b --- /dev/null +++ b/bin/report_modules/templates/ncbi_fcs_adaptor/report_contents.html @@ -0,0 +1,24 @@ +{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["NCBI_FCS_ADAPTOR"]|length) %} {% set +active_text = 'display: block' if vars.is_first else 'display: none' %} +
    +
    +
    +
    {{ all_stats_dicts['NCBI_FCS_ADAPTOR'][item]['hap'] }}
    +
    +
    + {%if all_stats_dicts['NCBI_FCS_ADAPTOR'][item]['did_detect_contamination'] %} +
    +

    Contamination detected. Subsequent pipeline modules are skipped.

    +
    +
    +
    {{ all_stats_dicts['NCBI_FCS_ADAPTOR'][item]['report_table_html'] }}
    +
    + {% else %} +

    No contamination detected.

    + {% endif %} +
    +{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/bin/report_modules/templates/ncbi_fcs_gx/dropdown.html b/bin/report_modules/templates/ncbi_fcs_gx/dropdown.html new file mode 100644 index 00000000..594f3e6f --- /dev/null +++ b/bin/report_modules/templates/ncbi_fcs_gx/dropdown.html @@ -0,0 +1,10 @@ +
    +
    + +
    +
    diff --git a/bin/report_modules/templates/ncbi_fcs_gx/ncbi_fcs_gx.html b/bin/report_modules/templates/ncbi_fcs_gx/ncbi_fcs_gx.html new file mode 100644 index 00000000..e2c2a338 --- /dev/null +++ b/bin/report_modules/templates/ncbi_fcs_gx/ncbi_fcs_gx.html @@ -0,0 +1,23 @@ + diff --git a/bin/report_modules/templates/ncbi_fcs_gx/report_contents.html b/bin/report_modules/templates/ncbi_fcs_gx/report_contents.html new file mode 100644 index 00000000..c99c5622 --- /dev/null +++ b/bin/report_modules/templates/ncbi_fcs_gx/report_contents.html @@ -0,0 +1,49 @@ +{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["NCBI_FCS_GX"]|length) %} {% set active_text = +'display: block' if vars.is_first else 'display: none' %} +
    +
    +
    +
    {{ all_stats_dicts['NCBI_FCS_GX'][item]['hap'] }}
    +
    + {%if all_stats_dicts['NCBI_FCS_GX'][item]['did_detect_contamination'] %} +
    +

    Contamination detected. Subsequent pipeline modules are skipped.

    +
    +
    +
    {{ all_stats_dicts['NCBI_FCS_GX'][item]['report_table_html'] }}
    +
    + {% else %} +
    +

    No contamination detected.

    +
    + {% endif %} {%if all_stats_dicts['NCBI_FCS_GX'][item]['is_wrong_div'] %} +
    +

    + Warning: Asserted div ({{ + all_stats_dicts['NCBI_FCS_GX'][item]['report_meta_data'][1]["run-info"]["asserted-div"] }}) is not + present in the inferred div(s) ({{ + all_stats_dicts['NCBI_FCS_GX'][item]['report_meta_data'][1]["run-info"]["inferred-primary-divs"] }}). + Either the tax ID is incorrect or the sample is predominantly contamination. +

    +
    + {% endif %} +
    +
    Taxonomy
    +
    +
    +

    Bogus, Repeat, Low-coverage and Inconclusive results are labelled as No hits.

    +
    +
    + +
    +
    +
    +{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/bin/report_modules/templates/params/contents.html b/bin/report_modules/templates/params/contents.html new file mode 100644 index 00000000..7c10ad16 --- /dev/null +++ b/bin/report_modules/templates/params/contents.html @@ -0,0 +1,8 @@ +
    +
    +
    +
    Pipeline Parameters
    +
    + {{ all_stats_dicts['PARAMS_TABLE'] }} +
    +
    diff --git a/bin/report_modules/templates/params/params.html b/bin/report_modules/templates/params/params.html new file mode 100644 index 00000000..276d1e15 --- /dev/null +++ b/bin/report_modules/templates/params/params.html @@ -0,0 +1,19 @@ + diff --git a/bin/report_modules/templates/tidk/dropdown.html b/bin/report_modules/templates/tidk/dropdown.html new file mode 100644 index 00000000..602bd367 --- /dev/null +++ b/bin/report_modules/templates/tidk/dropdown.html @@ -0,0 +1,11 @@ +
    +
    + +
    +
    diff --git a/bin/report_modules/templates/tidk/report_contents.html b/bin/report_modules/templates/tidk/report_contents.html new file mode 100644 index 00000000..9c81c00d --- /dev/null +++ b/bin/report_modules/templates/tidk/report_contents.html @@ -0,0 +1,33 @@ +{% set vars = {'is_first': True} %} {% for item in range(all_stats_dicts["TIDK"]|length) %} {% set active_text = +'display: block' if vars.is_first else 'display: none' %} +
    +
    +
    +
    {{ all_stats_dicts['TIDK'][item]['hap_display'] }}
    +
    + {% if all_stats_dicts['TIDK'][item]['has_sequence'] %} +
    +

    + Searched sequence: + {{ all_stats_dicts['TIDK'][item]['sequence'] }} +

    +
    + {% endif %} {% if all_stats_dicts['TIDK'][item]['is_a_priori'] %} +
    +

    + Searched sequence: + {{ all_stats_dicts['TIDK'][item]['a_priori_sequence'] }} +

    +
    + {% endif %} {% if all_stats_dicts['TIDK'][item]['tidk_plot_empty'] %} +
    +

    TIDK explore could not find a repeat sequence 😔

    +
    + {% else %} +
    + +
    + {% endif %} +
    +
    +{% if vars.update({'is_first': False}) %} {% endif %} {% endfor %} diff --git a/bin/report_modules/templates/tidk/tidk.html b/bin/report_modules/templates/tidk/tidk.html new file mode 100644 index 00000000..0503dae9 --- /dev/null +++ b/bin/report_modules/templates/tidk/tidk.html @@ -0,0 +1,15 @@ + diff --git a/bin/report_modules/templates/tools/contents.html b/bin/report_modules/templates/tools/contents.html new file mode 100644 index 00000000..721721b6 --- /dev/null +++ b/bin/report_modules/templates/tools/contents.html @@ -0,0 +1,9 @@ +
    +
    +
    +
    Pipeline Tools
    +

    Following is a non-exhaustive list of tools used to generate this report.

    +
    + {{ all_stats_dicts['TOOLS_TABLE'] }} +
    +
    diff --git a/bin/report_modules/templates/tools/tools.html b/bin/report_modules/templates/tools/tools.html new file mode 100644 index 00000000..4d78232c --- /dev/null +++ b/bin/report_modules/templates/tools/tools.html @@ -0,0 +1 @@ + diff --git a/conf/modules.config b/conf/modules.config index 488c19c0..0b1ba155 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -20,9 +20,6 @@ process { ] } -} - -process { withName: ASSEMBLATHON_STATS { publishDir = [ path: { "${params.outdir}/assemblathon_stats" }, @@ -94,10 +91,7 @@ process { saveAs: { filename -> filename.equals("versions.yml") ? null : filename } ] } -} - -process { withName: '.*:GFF3_VALIDATE:GT_GFF3' { ext.args = '-tidy -retainids' } @@ -119,9 +113,6 @@ process { saveAs: { filename -> filename.equals("versions.yml") ? null : filename } ] } -} - -process { withName: FILTER_BY_LENGTH { ext.args = params.tidk_filter_by_size ? "-m ${params.tidk_filter_size_bp}" : '' @@ -179,10 +170,6 @@ process { saveAs: { filename -> filename.equals("versions.yml") ? null : filename } ] } -} - - -process { withName: CUSTOM_SHORTENFASTAIDS { publishDir = [ @@ -227,9 +214,6 @@ process { saveAs: { filename -> filename.equals("versions.yml") ? null : filename } ] } -} - -process { withName: FASTQC_RAW { publishDir = [ @@ -305,4 +289,12 @@ process { saveAs: { filename -> filename.equals("versions.yml") ? null : filename } ] } + + withName: CREATEREPORT { + publishDir = [ + path: { "$params.outdir" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals("versions.yml") ? null : filename } + ] + } } diff --git a/lib/WorkflowAssemblyqc.groovy b/lib/WorkflowAssemblyqc.groovy index 2c45d1e2..c9afe5ad 100755 --- a/lib/WorkflowAssemblyqc.groovy +++ b/lib/WorkflowAssemblyqc.groovy @@ -4,11 +4,40 @@ import nextflow.Nextflow import groovy.text.SimpleTemplateEngine +import groovy.json.JsonOutput class WorkflowAssemblyqc { // // Check and validate parameters // - public static void initialise(params, log) { } + public static void initialise(params, log) { + // Check for ncbi_fcs_gx_tax_id + if (!params.ncbi_fcs_gx_skip && !params.ncbi_fcs_gx_tax_id) { + log.error('ncbi_fcs_gx_tax_id must be provided when executing NCBI FCS GX') + System.exit(1) + } + + // Check for ncbi_fcs_gx_db_path + if (!params.ncbi_fcs_gx_skip && !params.ncbi_fcs_gx_db_path) { + log.error('ncbi_fcs_gx_db_path must be provided when executing NCBI FCS GX') + System.exit(1) + } + + // Check for busco_lineage_datasets + if (!params.busco_skip && !params.busco_lineage_datasets) { + log.error('busco_lineage_datasets must be provided when executing BUSCO') + System.exit(1) + } + + // Check for kraken2_db_path + if (!params.kraken2_skip && !params.kraken2_db_path) { + log.error('kraken2_db_path must be provided when executing Kraken2') + System.exit(1) + } + } + + public static String jsonifyParams(params) { + return JsonOutput.toJson(params) + } } diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy index 2b6cfbbe..abfc7acf 100755 --- a/lib/WorkflowMain.groovy +++ b/lib/WorkflowMain.groovy @@ -42,26 +42,6 @@ class WorkflowMain { // Check AWS batch settings NfcoreTemplate.awsBatch(workflow, params) - - // Check for ncbi_fcs_gx_tax_id - if (!params.ncbi_fcs_gx_skip && !params.ncbi_fcs_gx_tax_id) { - Nextflow.error('ncbi_fcs_gx_tax_id must be provided when executing NCBI FCS GX') - } - - // Check for ncbi_fcs_gx_db_path - if (!params.ncbi_fcs_gx_skip && !params.ncbi_fcs_gx_db_path) { - Nextflow.error('ncbi_fcs_gx_db_path must be provided when executing NCBI FCS GX') - } - - // Check for busco_lineage_datasets - if (!params.busco_skip && !params.busco_lineage_datasets) { - Nextflow.error('busco_lineage_datasets must be provided when executing BUSCO') - } - - // Check for kraken2_db_path - if (!params.kraken2_skip && !params.kraken2_db_path) { - Nextflow.error('kraken2_db_path must be provided when executing Kraken2') - } } // // Get attribute from genome config file e.g. fasta diff --git a/modules/local/createreport.nf b/modules/local/createreport.nf new file mode 100644 index 00000000..41c69b24 --- /dev/null +++ b/modules/local/createreport.nf @@ -0,0 +1,38 @@ +process CREATEREPORT { + tag "AssemblyQC" + label 'process_single' + + // container "docker.io/gallvp/python3npkgs:v0.4" + + input: + path fastavalidator_logs, stageAs: 'fastavalidator_logs/*' + path gff3_validate_logs, stageAs: 'gff3_validate_logs/*' + path ncbi_fcs_adaptor_reports, stageAs: 'ncbi_fcs_adaptor_reports/*' + path fcs_gx_reports, stageAs: 'fcs_gx_reports/*' + path assemblathon_stats, stageAs: 'assemblathon_stats/*' + path genometools_gt_stats, stageAs: 'genometools_gt_stat/*' + path busco_outputs, stageAs: 'busco_outputs/*' + path tidk_plots, stageAs: 'tidk_outputs/*' + path lai_outputs, stageAs: 'lai_outputs/*' + path kraken2_outputs, stageAs: 'kraken2_outputs/*' + path hic_outputs, stageAs: 'hic_outputs/*' + path circos_outputs, stageAs: 'circos_outputs/*' + path versions + val params_json + + output: + path 'report.html' + path 'report.json' + + script: + """ + echo -n '$params_json' > params_json.json + assemblyqc.py > report.html + """ + + stub: + """ + touch report.html + touch report.json + """ +} diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index 0854028e..c4e60287 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -37,6 +37,7 @@ include { FASTA_LTRRETRIEVER_LAI } from '../subworkflows/pfr/fasta_lt include { FASTA_KRAKEN2 } from '../subworkflows/local/fasta_kraken2' include { FQ2HIC } from '../subworkflows/local/fq2hic' include { FASTA_SYNTENY } from '../subworkflows/local/fasta_synteny' +include { CREATEREPORT } from '../modules/local/createreport' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -421,6 +422,24 @@ workflow ASSEMBLYQC { CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') ) + + // MODULE: CREATEREPORT + CREATEREPORT( + ch_invalid_assembly_log .map { meta, file -> file }.collect().ifEmpty([]), + ch_invalid_gff3_log .map { meta, file -> file }.collect().ifEmpty([]), + ch_fcs_adaptor_report .map { meta, file -> file }.collect().ifEmpty([]), + ch_fcs_gx_report .mix(ch_fcs_gx_taxonomy_plot).map { meta, file -> file }.collect().ifEmpty([]), + ch_assemblathon_stats .collect().ifEmpty([]), + ch_gt_stats .collect().ifEmpty([]), + ch_busco_summary .mix(ch_busco_plot).collect().ifEmpty([]), + ch_tidk_outputs .collect().ifEmpty([]), + ch_lai_outputs .collect().ifEmpty([]), + ch_kraken2_plot .collect().ifEmpty([]), + ch_hic_html .collect().ifEmpty([]), + ch_synteny_plot .collect().ifEmpty([]), + CUSTOM_DUMPSOFTWAREVERSIONS .out.yml, + Channel.of ( WorkflowAssemblyqc.jsonifyParams ( params ) ), + ) } /* From 93e7715d5bae153c2a4b26e786febe5dcf8c7884 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 27 Feb 2024 20:55:38 +1300 Subject: [PATCH 33/65] Fixed version links in the report --- .../templates/assemblathon_stats/assemblathon_stats.html | 2 +- bin/report_modules/templates/base.html | 2 +- bin/report_modules/templates/busco/busco.html | 2 +- bin/report_modules/templates/circos/circos.html | 4 ++-- bin/report_modules/templates/kraken2/kraken2.html | 2 +- bin/report_modules/templates/lai/lai.html | 8 +------- bin/report_modules/templates/params/params.html | 6 +++--- 7 files changed, 10 insertions(+), 16 deletions(-) diff --git a/bin/report_modules/templates/assemblathon_stats/assemblathon_stats.html b/bin/report_modules/templates/assemblathon_stats/assemblathon_stats.html index d8e5b82d..356b0eaf 100644 --- a/bin/report_modules/templates/assemblathon_stats/assemblathon_stats.html +++ b/bin/report_modules/templates/assemblathon_stats/assemblathon_stats.html @@ -13,7 +13,7 @@

    Warning:

    Contig-related stats are based on the assumption that the n_limit ({{ - all_stats_dicts['PARAMS_DICT']['assemblathon_stats']['n_limit'] }}) parameter is specified correctly. If you + all_stats_dicts['PARAMS_DICT']['assemblathon_stats_n_limit'] }}) parameter is specified correctly. If you are not certain of the value of the n_limit parameter, please ignore the contig-related stats.

    diff --git a/bin/report_modules/templates/base.html b/bin/report_modules/templates/base.html index e0b8eef0..8766284c 100644 --- a/bin/report_modules/templates/base.html +++ b/bin/report_modules/templates/base.html @@ -3,7 +3,7 @@
    -
    AssemblyQC {{ all_stats_dicts['VERSIONS']['Workflow']['AssemblyQC'] }}
    +
    AssemblyQC {{ all_stats_dicts['VERSIONS']['Workflow']['plant-food-research-open/assemblyqc'] }}
    diff --git a/bin/report_modules/templates/busco/busco.html b/bin/report_modules/templates/busco/busco.html index 06d560e5..4f6f9b3a 100644 --- a/bin/report_modules/templates/busco/busco.html +++ b/bin/report_modules/templates/busco/busco.html @@ -11,7 +11,7 @@ viral genomes. arXiv:2106.11799 [q-bio] [Internet]. Available from: arxiv.org/abs/2106.11799

    -

    Version: {{ all_stats_dicts['VERSIONS']['RUN_BUSCO']['busco'] }}

    +

    Version: {{ all_stats_dicts['VERSIONS']['BUSCO']['busco'] }}

    {% include 'busco/dropdown.html' %} {% include 'busco/summary_contents.html' %} {% include 'busco/report_contents.html' %} diff --git a/bin/report_modules/templates/circos/circos.html b/bin/report_modules/templates/circos/circos.html index 2099a251..84808348 100644 --- a/bin/report_modules/templates/circos/circos.html +++ b/bin/report_modules/templates/circos/circos.html @@ -23,9 +23,9 @@

    Notes:

    - Alignments within a distance of {{ all_stats_dicts['PARAMS_DICT']['synteny']['max_gap'] }}bp have been + Alignments within a distance of {{ all_stats_dicts['PARAMS_DICT']['synteny_max_gap'] }}bp have been bundled together. After bundling, any bundle smaller than {{ - all_stats_dicts['PARAMS_DICT']['synteny']['min_bundle_size'] }}bp has been filtered out. + all_stats_dicts['PARAMS_DICT']['synteny_min_bundle_size'] }}bp has been filtered out.

    {% include 'circos/dropdown.html' %} {% include 'circos/report_contents.html' %} diff --git a/bin/report_modules/templates/kraken2/kraken2.html b/bin/report_modules/templates/kraken2/kraken2.html index 1b87e9e2..522df1d8 100644 --- a/bin/report_modules/templates/kraken2/kraken2.html +++ b/bin/report_modules/templates/kraken2/kraken2.html @@ -9,7 +9,7 @@ Wood, D.E., Lu, J. & Langmead, B. Improved metagenomic analysis with Kraken 2. Genome Biol 20, 257 (2019). 10.1186/s13059-019-1891-0

    -

    Version: {{ all_stats_dicts['VERSIONS']['RUN_KRAKEN2']['kraken2'] }}

    +

    Version: {{ all_stats_dicts['VERSIONS']['KRAKEN2']['kraken2'] }}

    {% include 'kraken2/dropdown.html' %} {% include 'kraken2/report_contents.html' %} diff --git a/bin/report_modules/templates/lai/lai.html b/bin/report_modules/templates/lai/lai.html index 4b0775ae..50a98582 100644 --- a/bin/report_modules/templates/lai/lai.html +++ b/bin/report_modules/templates/lai/lai.html @@ -14,13 +14,7 @@ Nucleic Acids Research, Volume 46, Issue 21, 30 November 2018, Page e126, 10.1093/nar/gky730

    -

    Version: {{ all_stats_dicts['VERSIONS']['LAI']['lai'] }}

    - {% if all_stats_dicts['PARAMS_DICT']['lai']['mode'] == '-qq' %} -

    Warning:

    -

    - The LAI mode is set to '-qq' and, therefore, the RAW LAI can only be used for intra-specific comparisons. -

    - {% endif %} +

    Version: {{ all_stats_dicts['VERSIONS']['LTRRETRIEVER_LAI']['lai'] }}

    {% include 'lai/dropdown.html' %} {% include 'lai/summary_contents.html' %} diff --git a/bin/report_modules/templates/params/params.html b/bin/report_modules/templates/params/params.html index 276d1e15..70452246 100644 --- a/bin/report_modules/templates/params/params.html +++ b/bin/report_modules/templates/params/params.html @@ -8,10 +8,10 @@

    Rashid, U., Wu, C., Shiller, J., Smith, K., Crowhurst, R., Davy, M., Chen, T.-H., Thomson, S., & Deng, C. (2024). AssemblyQC: A NextFlow pipeline for evaluating assembly quality ({{ - all_stats_dicts['VERSIONS']['Workflow']['AssemblyQC'] }}). Zenodo. + all_stats_dicts['VERSIONS']['Workflow']['plant-food-research-open/assemblyqc'] }}). Zenodo. 10.5281/zenodo.10647870. GitHub. - https://github.com/Plant-Food-Research-Open/assembly_qchttps://github.com/Plant-Food-Research-Open/assemblyqc

    From 9716c76f03dd0c61e5e0b1faadf1f0ec82112e56 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 27 Feb 2024 21:54:58 +1300 Subject: [PATCH 34/65] Only printing non-default params in the report --- .../templates/params/contents.html | 1 + lib/WorkflowAssemblyqc.groovy | 22 ++++++++++++++++++- nextflow.config | 6 ++--- nextflow_schema.json | 6 ++--- workflows/assemblyqc.nf | 6 ++--- 5 files changed, 30 insertions(+), 11 deletions(-) diff --git a/bin/report_modules/templates/params/contents.html b/bin/report_modules/templates/params/contents.html index 7c10ad16..10115c8d 100644 --- a/bin/report_modules/templates/params/contents.html +++ b/bin/report_modules/templates/params/contents.html @@ -3,6 +3,7 @@
    Pipeline Parameters
    +

    Only displaying parameters that differ from the pipeline defaults.

    {{ all_stats_dicts['PARAMS_TABLE'] }} diff --git a/lib/WorkflowAssemblyqc.groovy b/lib/WorkflowAssemblyqc.groovy index c9afe5ad..1d04056a 100755 --- a/lib/WorkflowAssemblyqc.groovy +++ b/lib/WorkflowAssemblyqc.groovy @@ -12,6 +12,12 @@ class WorkflowAssemblyqc { // Check and validate parameters // public static void initialise(params, log) { + // Check for ncbi_fcs_adaptor_empire + if (!params.ncbi_fcs_adaptor_skip && !params.ncbi_fcs_adaptor_empire) { + log.error('ncbi_fcs_adaptor_empire must be provided when executing NCBI FCS Adaptor') + System.exit(1) + } + // Check for ncbi_fcs_gx_tax_id if (!params.ncbi_fcs_gx_skip && !params.ncbi_fcs_gx_tax_id) { log.error('ncbi_fcs_gx_tax_id must be provided when executing NCBI FCS GX') @@ -24,6 +30,12 @@ class WorkflowAssemblyqc { System.exit(1) } + // Check for busco_mode + if (!params.busco_skip && !params.busco_mode) { + log.error("busco_mode must be provided when executing BUSCO") + System.exit(1) + } + // Check for busco_lineage_datasets if (!params.busco_skip && !params.busco_lineage_datasets) { log.error('busco_lineage_datasets must be provided when executing BUSCO') @@ -38,6 +50,14 @@ class WorkflowAssemblyqc { } public static String jsonifyParams(params) { - return JsonOutput.toJson(params) + + def summary = [:] + for (group in params.keySet()) { + for (subgroup in params[group].keySet()) { + if ( params[group][subgroup] ) { summary << [ "$subgroup": "${params[group][subgroup]}" ] } + } + } + + return JsonOutput.toJson(summary).toString() } } diff --git a/nextflow.config b/nextflow.config index 97372502..795a3f41 100644 --- a/nextflow.config +++ b/nextflow.config @@ -17,7 +17,7 @@ params { // NCBI FCS options ncbi_fcs_adaptor_skip = true - ncbi_fcs_adaptor_empire = 'euk' + ncbi_fcs_adaptor_empire = null ncbi_fcs_gx_skip = true ncbi_fcs_gx_tax_id = null @@ -25,13 +25,13 @@ params { // BUSCO options busco_skip = true - busco_mode = 'geno' + busco_mode = null busco_lineage_datasets = null busco_download_path = null // TIDK options tidk_skip = true - tidk_repeat_seq = 'TTAGGG' + tidk_repeat_seq = null tidk_filter_by_size = false tidk_filter_size_bp = 1000000 diff --git a/nextflow_schema.json b/nextflow_schema.json index 69f67e0a..8bfbc274 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -64,9 +64,9 @@ }, "ncbi_fcs_adaptor_empire": { "type": "string", - "default": "euk", "enum": ["euk", "prok"], - "description": "Empire for NCBI FCS Adaptor checking" + "description": "Empire for NCBI FCS Adaptor checking", + "help_text": "'euk' for eukaryotes, or 'prok' for prokaryotes" }, "ncbi_fcs_gx_skip": { "type": "boolean", @@ -99,7 +99,6 @@ }, "busco_mode": { "type": "string", - "default": "geno", "enum": ["geno", "tran", "prot", "genome", "transcriptome", "proteins"], "help_text": "'geno' or 'genome' for genome assemblies (DNA), 'tran' or 'transcriptome' for transcriptome assemblies (DNA), 'prot' or 'proteins' for annotated gene sets (protein)", "description": "BUSCO mode" @@ -131,7 +130,6 @@ }, "tidk_repeat_seq": { "type": "string", - "default": "TTAGGG", "description": "Telomere repeat sequence", "pattern": "^[ACGT]+$" }, diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index c4e60287..198e106a 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -191,7 +191,7 @@ workflow ASSEMBLYQC { NCBI_FCS_ADAPTOR( ch_fcs_adaptor_inputs, - params.ncbi_fcs_adaptor_empire + params.ncbi_fcs_adaptor_empire ?: [] ) ch_fcs_adaptor_report = NCBI_FCS_ADAPTOR.out.report @@ -314,7 +314,7 @@ workflow ASSEMBLYQC { FASTA_BUSCO_PLOT( ch_busco_inputs.map { tag, fa, lineage -> [ tag, fa ] }, ch_busco_inputs.map { tag, fa, lineage -> lineage }, - params.busco_mode, + params.busco_mode ?: [], params.busco_download_path ?: [] ) @@ -438,7 +438,7 @@ workflow ASSEMBLYQC { ch_hic_html .collect().ifEmpty([]), ch_synteny_plot .collect().ifEmpty([]), CUSTOM_DUMPSOFTWAREVERSIONS .out.yml, - Channel.of ( WorkflowAssemblyqc.jsonifyParams ( params ) ), + Channel.of ( WorkflowAssemblyqc.jsonifyParams ( summary_params ) ) ) } From 624090177792d0143eb4a29326e05e442b023ac2 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 27 Feb 2024 22:06:54 +1300 Subject: [PATCH 35/65] Added additional params to stub test config --- lib/WorkflowAssemblyqc.groovy | 6 ++++++ nextflow_schema.json | 3 ++- tests/stub/stub.config | 3 +++ 3 files changed, 11 insertions(+), 1 deletion(-) diff --git a/lib/WorkflowAssemblyqc.groovy b/lib/WorkflowAssemblyqc.groovy index 1d04056a..7a11ebeb 100755 --- a/lib/WorkflowAssemblyqc.groovy +++ b/lib/WorkflowAssemblyqc.groovy @@ -42,6 +42,12 @@ class WorkflowAssemblyqc { System.exit(1) } + // Check for tidk_repeat_seq + if (!params.tidk_skip && !params.tidk_repeat_seq) { + log.error('tidk_repeat_seq must be provided when executing TIDK') + System.exit(1) + } + // Check for kraken2_db_path if (!params.kraken2_skip && !params.kraken2_db_path) { log.error('kraken2_db_path must be provided when executing Kraken2') diff --git a/nextflow_schema.json b/nextflow_schema.json index 8bfbc274..3299f676 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -131,7 +131,8 @@ "tidk_repeat_seq": { "type": "string", "description": "Telomere repeat sequence", - "pattern": "^[ACGT]+$" + "pattern": "^[ACGT]+$", + "help_text": "Plant: TTTAGGG, Fungus, Vertebrates: TTAGGG, Insect: TTAGG" }, "tidk_filter_by_size": { "type": "boolean", diff --git a/tests/stub/stub.config b/tests/stub/stub.config index 8f0f03b4..c373c516 100644 --- a/tests/stub/stub.config +++ b/tests/stub/stub.config @@ -5,15 +5,18 @@ params { input = 'tests/stub/assemblysheet.csv' ncbi_fcs_adaptor_skip = false + ncbi_fcs_adaptor_empire = 'euk' ncbi_fcs_gx_skip = false ncbi_fcs_gx_tax_id = 12 ncbi_fcs_gx_db_path = 'tests/stub/gxdb/test' busco_skip = false + busco_mode = 'geno' busco_lineage_datasets = 'fungi_odb10 hypocreales_odb10' tidk_skip = false + tidk_repeat_seq = 'TTTGGG' lai_skip = false From 294c4a2fc28cb30ccbe792ecf22318413d5b04b6 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 28 Feb 2024 10:51:45 +1300 Subject: [PATCH 36/65] Fixed linting issues --- lib/WorkflowAssemblyqc.groovy | 21 +++++++-------------- modules/local/createreport.nf | 20 ++++++++++++++++++-- 2 files changed, 25 insertions(+), 16 deletions(-) diff --git a/lib/WorkflowAssemblyqc.groovy b/lib/WorkflowAssemblyqc.groovy index 7a11ebeb..e5b77980 100755 --- a/lib/WorkflowAssemblyqc.groovy +++ b/lib/WorkflowAssemblyqc.groovy @@ -14,44 +14,37 @@ class WorkflowAssemblyqc { public static void initialise(params, log) { // Check for ncbi_fcs_adaptor_empire if (!params.ncbi_fcs_adaptor_skip && !params.ncbi_fcs_adaptor_empire) { - log.error('ncbi_fcs_adaptor_empire must be provided when executing NCBI FCS Adaptor') - System.exit(1) + Nextflow.error('ncbi_fcs_adaptor_empire must be provided when executing NCBI FCS Adaptor') } // Check for ncbi_fcs_gx_tax_id if (!params.ncbi_fcs_gx_skip && !params.ncbi_fcs_gx_tax_id) { - log.error('ncbi_fcs_gx_tax_id must be provided when executing NCBI FCS GX') - System.exit(1) + Nextflow.error('ncbi_fcs_gx_tax_id must be provided when executing NCBI FCS GX') } // Check for ncbi_fcs_gx_db_path if (!params.ncbi_fcs_gx_skip && !params.ncbi_fcs_gx_db_path) { - log.error('ncbi_fcs_gx_db_path must be provided when executing NCBI FCS GX') - System.exit(1) + Nextflow.error('ncbi_fcs_gx_db_path must be provided when executing NCBI FCS GX') } // Check for busco_mode if (!params.busco_skip && !params.busco_mode) { - log.error("busco_mode must be provided when executing BUSCO") - System.exit(1) + Nextflow.error("busco_mode must be provided when executing BUSCO") } // Check for busco_lineage_datasets if (!params.busco_skip && !params.busco_lineage_datasets) { - log.error('busco_lineage_datasets must be provided when executing BUSCO') - System.exit(1) + Nextflow.error('busco_lineage_datasets must be provided when executing BUSCO') } // Check for tidk_repeat_seq if (!params.tidk_skip && !params.tidk_repeat_seq) { - log.error('tidk_repeat_seq must be provided when executing TIDK') - System.exit(1) + Nextflow.error('tidk_repeat_seq must be provided when executing TIDK') } // Check for kraken2_db_path if (!params.kraken2_skip && !params.kraken2_db_path) { - log.error('kraken2_db_path must be provided when executing Kraken2') - System.exit(1) + Nextflow.error('kraken2_db_path must be provided when executing Kraken2') } } diff --git a/modules/local/createreport.nf b/modules/local/createreport.nf index 41c69b24..29ea765c 100644 --- a/modules/local/createreport.nf +++ b/modules/local/createreport.nf @@ -21,18 +21,34 @@ process CREATEREPORT { val params_json output: - path 'report.html' - path 'report.json' + path 'report.html' , emit: html + path 'report.json' , emit: json + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when script: """ echo -n '$params_json' > params_json.json assemblyqc.py > report.html + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + python: \$(python --version | tr -d 'Python[:space:]') + pandas: \$(python -c "import pandas; print(pandas.__version__)") + END_VERSIONS """ stub: """ touch report.html touch report.json + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + python: \$(python --version | tr -d 'Python[:space:]') + pandas: \$(python -c "import pandas; print(pandas.__version__)") + END_VERSIONS """ } From 321de3084069435731da560c37a2ba6e6ce2d52a Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 28 Feb 2024 11:22:15 +1300 Subject: [PATCH 37/65] Fixed reporting issues --- bin/assemblyqc.py | 7 ++++- bin/report_modules/parsers/params_parser.py | 29 ++----------------- .../templates/params/contents.html | 2 +- lib/WorkflowAssemblyqc.groovy | 4 +++ modules/local/createreport.nf | 19 +++++++++--- workflows/assemblyqc.nf | 3 +- 6 files changed, 31 insertions(+), 33 deletions(-) diff --git a/bin/assemblyqc.py b/bin/assemblyqc.py index 99eb2d73..fc42eeed 100755 --- a/bin/assemblyqc.py +++ b/bin/assemblyqc.py @@ -27,7 +27,10 @@ from report_modules.parsers.circos_parser import parse_circos_folder if __name__ == "__main__": - params_dict, params_table = parse_params_json() + params_dict, params_table = parse_params_json("params_json.json") + params_summary_dict, params_summary_table = parse_params_json( + "params_summary_json.json" + ) tools_dict, tools_table = parse_tools_yaml() data_from_tools = {} @@ -51,6 +54,8 @@ data_from_tools = { "PARAMS_DICT": params_dict, "PARAMS_TABLE": params_table, + "PARAMS_SUMMARY_DICT": params_summary_dict, + "PARAMS_SUMMARY_TABLE": params_summary_table, "TOOLS_DICT": tools_dict, "TOOLS_TABLE": tools_table, "VERSIONS": { diff --git a/bin/report_modules/parsers/params_parser.py b/bin/report_modules/parsers/params_parser.py index bf07dc5a..7806f18f 100644 --- a/bin/report_modules/parsers/params_parser.py +++ b/bin/report_modules/parsers/params_parser.py @@ -12,32 +12,9 @@ def highlight_json(json_string): return highlight(json_string, lexer, formatter) -def format_params_dict(json_dict): - formatted_dict = {} - for key, value in json_dict.items(): - if key in ["max_cpus", "max_memory", "max_time"]: - continue - - if not isinstance(value, dict): - formatted_dict[key] = value - continue - - if "skip" in value.keys(): - if value["skip"] == 1: - formatted_dict[key] = "Skipped" - continue - - formatted_dict[key] = value - formatted_dict[key].pop("skip", None) - - return formatted_dict - - -def parse_params_json(): - with open("params_json.json", "r") as f: +def parse_params_json(file_name): + with open(file_name, "r") as f: params_dict = json.load(f) - formatted_dict_json = highlight_json( - json.dumps(format_params_dict(params_dict), indent=4) - ) + formatted_dict_json = highlight_json(json.dumps(params_dict, indent=4)) return params_dict, formatted_dict_json diff --git a/bin/report_modules/templates/params/contents.html b/bin/report_modules/templates/params/contents.html index 10115c8d..f89d27dd 100644 --- a/bin/report_modules/templates/params/contents.html +++ b/bin/report_modules/templates/params/contents.html @@ -4,6 +4,6 @@
    Pipeline Parameters

    Only displaying parameters that differ from the pipeline defaults.

    - {{ all_stats_dicts['PARAMS_TABLE'] }} + {{ all_stats_dicts['PARAMS_SUMMARY_TABLE'] }} diff --git a/lib/WorkflowAssemblyqc.groovy b/lib/WorkflowAssemblyqc.groovy index e5b77980..c410665b 100755 --- a/lib/WorkflowAssemblyqc.groovy +++ b/lib/WorkflowAssemblyqc.groovy @@ -49,6 +49,10 @@ class WorkflowAssemblyqc { } public static String jsonifyParams(params) { + return JsonOutput.toJson(params).toString() + } + + public static String jsonifySummaryParams(params) { def summary = [:] for (group in params.keySet()) { diff --git a/modules/local/createreport.nf b/modules/local/createreport.nf index 29ea765c..820f106e 100644 --- a/modules/local/createreport.nf +++ b/modules/local/createreport.nf @@ -2,7 +2,7 @@ process CREATEREPORT { tag "AssemblyQC" label 'process_single' - // container "docker.io/gallvp/python3npkgs:v0.4" + container "docker.io/gallvp/python3npkgs:v0.6" input: path fastavalidator_logs, stageAs: 'fastavalidator_logs/*' @@ -19,19 +19,30 @@ process CREATEREPORT { path circos_outputs, stageAs: 'circos_outputs/*' path versions val params_json + val params_summary_json output: path 'report.html' , emit: html path 'report.json' , emit: json - path "versions.yml" , emit: versions + path 'versions.yml' , emit: versions when: task.ext.when == null || task.ext.when script: """ - echo -n '$params_json' > params_json.json - assemblyqc.py > report.html + echo \\ + -n \\ + '$params_json' \\ + > params_json.json + + echo \\ + -n \\ + '$params_summary_json' \\ + > params_summary_json.json + + assemblyqc.py \\ + > report.html cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index 198e106a..17ca3f67 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -438,7 +438,8 @@ workflow ASSEMBLYQC { ch_hic_html .collect().ifEmpty([]), ch_synteny_plot .collect().ifEmpty([]), CUSTOM_DUMPSOFTWAREVERSIONS .out.yml, - Channel.of ( WorkflowAssemblyqc.jsonifyParams ( summary_params ) ) + Channel.of ( WorkflowAssemblyqc.jsonifyParams ( params ) ), + Channel.of ( WorkflowAssemblyqc.jsonifySummaryParams ( summary_params ) ) ) } From 53ff084de9efeeb8befde9a64ee88da850c540ba Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 28 Feb 2024 11:41:23 +1300 Subject: [PATCH 38/65] Removed TODO from base.conf --- conf/base.config | 2 -- 1 file changed, 2 deletions(-) diff --git a/conf/base.config b/conf/base.config index c632c1ef..c7f00271 100644 --- a/conf/base.config +++ b/conf/base.config @@ -23,8 +23,6 @@ process { // These labels are used and recognised by default in DSL2 files hosted on nf-core/modules. // If possible, it would be nice to keep the same label naming convention when // adding in your local modules too. - // TODO nf-core: Customise requirements for specific processes. - // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors withLabel:process_single { cpus = { check_max( 1 , 'cpus' ) } memory = { check_max( 6.GB * task.attempt, 'memory' ) } From 27b746fe1ee7ae0ba3e08b8dff4e2dffbc2f4e94 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 28 Feb 2024 11:43:38 +1300 Subject: [PATCH 39/65] Updated authors in the license --- LICENSE | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/LICENSE b/LICENSE index bee1bb59..96e3eb88 100644 --- a/LICENSE +++ b/LICENSE @@ -1,6 +1,6 @@ MIT License -Copyright (c) Usman Rashid +Copyright (c) Usman Rashid, Ken Smith, Ross Crowhurst, Chen Wu, Marcus Davy Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal From efdac55a125fb7da40542a45f779fdad22063568 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 28 Feb 2024 12:13:50 +1300 Subject: [PATCH 40/65] Moved file for profile test_full --- assets/assemblysheet.csv | 2 +- {tests/stub => assets}/xrefsheet.csv | 0 conf/test.config | 14 ++++++------- conf/test_full.config | 30 +++++++++++++++++++++++++--- tests/stub/assemblysheet.csv | 2 -- tests/stub/stub.config | 4 ++-- 6 files changed, 37 insertions(+), 15 deletions(-) rename {tests/stub => assets}/xrefsheet.csv (100%) delete mode 100644 tests/stub/assemblysheet.csv diff --git a/assets/assemblysheet.csv b/assets/assemblysheet.csv index 19333351..06b8a783 100644 --- a/assets/assemblysheet.csv +++ b/assets/assemblysheet.csv @@ -1,2 +1,2 @@ tag,fasta,gff3,monoploid_ids,synteny_labels -FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz,, +FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz,https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/tests/stub/FI1.monoploid.seqs.txt,https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/tests/stub/FI1.seq.labels.tsv diff --git a/tests/stub/xrefsheet.csv b/assets/xrefsheet.csv similarity index 100% rename from tests/stub/xrefsheet.csv rename to assets/xrefsheet.csv diff --git a/conf/test.config b/conf/test.config index 4e55a764..3ba5bef2 100644 --- a/conf/test.config +++ b/conf/test.config @@ -11,13 +11,13 @@ */ params { - config_profile_name = 'Test profile' - config_profile_description = 'Minimal test dataset to check pipeline function' + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test dataset to check pipeline function' - // Limit resources so that this can run on GitHub Actions - max_cpus = 2 - max_memory = '6.GB' - max_time = '6.h' + input = 'https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/assemblysheet.csv' - input = 'https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/assemblysheet.csv' + // Limit resources so that this can run on GitHub Actions + max_cpus = 2 + max_memory = '6.GB' + max_time = '6.h' } diff --git a/conf/test_full.config b/conf/test_full.config index 1232442e..31b136c8 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -11,8 +11,32 @@ */ params { - config_profile_name = 'Full test profile' - config_profile_description = 'Full test dataset to check pipeline function' + config_profile_name = 'Full test profile' + config_profile_description = 'Full test dataset to check pipeline function' - input = 'https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/assemblysheet.csv' + input = 'https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/assemblysheet.csv' + + ncbi_fcs_adaptor_skip = false + ncbi_fcs_adaptor_empire = 'euk' + + ncbi_fcs_gx_skip = true // Skipping this step as the dataset is humengous (500 GB). Please download the dataset manually + // ncbi_fcs_gx_tax_id = 35717 + // ncbi_fcs_gx_db_path = 'https://ftp.ncbi.nlm.nih.gov/genomes/TOOLS/FCS/database/r2023-01-24' + + busco_skip = false + busco_mode = 'geno' + busco_lineage_datasets = 'fungi_odb10 hypocreales_odb10' + + tidk_skip = false + tidk_repeat_seq = 'TTTGGG' + + lai_skip = false + + kraken2_skip = true // Skipping this step as the dataset is humengous (126 GB). Please download the dataset manually + // kraken2_db_path = 'https://genome-idx.s3.amazonaws.com/kraken/k2_pluspfp_20240112.tar.gz' + + hic = 'SRR8238190' + + synteny_skip = false + synteny_xref_assemblies = 'tests/stub/xrefsheet.csv' } diff --git a/tests/stub/assemblysheet.csv b/tests/stub/assemblysheet.csv deleted file mode 100644 index 06b8a783..00000000 --- a/tests/stub/assemblysheet.csv +++ /dev/null @@ -1,2 +0,0 @@ -tag,fasta,gff3,monoploid_ids,synteny_labels -FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz,https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/tests/stub/FI1.monoploid.seqs.txt,https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/tests/stub/FI1.seq.labels.tsv diff --git a/tests/stub/stub.config b/tests/stub/stub.config index c373c516..cf626085 100644 --- a/tests/stub/stub.config +++ b/tests/stub/stub.config @@ -2,7 +2,7 @@ params { config_profile_name = 'Full stub test' config_profile_description = 'Full test of the pipeline in stub mode' - input = 'tests/stub/assemblysheet.csv' + input = 'https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/assemblysheet.csv' ncbi_fcs_adaptor_skip = false ncbi_fcs_adaptor_empire = 'euk' @@ -26,7 +26,7 @@ params { hic = 'tests/stub/hic/Dummy_hic.R{1,2}.fq.gz' synteny_skip = false - synteny_xref_assemblies = 'tests/stub/xrefsheet.csv' + synteny_xref_assemblies = 'https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/xrefsheet.csv' // Limit resources so that this can run on GitHub Actions max_cpus = 2 From 0d85347dc48d6994c62a1c1a96d6b77f5bb7235f Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 28 Feb 2024 17:05:45 +1300 Subject: [PATCH 41/65] Fixed minor issues to support test_full --- bin/report_modules/parsers/busco_parser.py | 13 +++++++------ conf/test_full.config | 2 +- modules/local/busco.nf | 1 - modules/local/ncbi_fcs_adaptor.nf | 8 ++++---- nextflow.config | 5 +++-- 5 files changed, 15 insertions(+), 14 deletions(-) diff --git a/bin/report_modules/parsers/busco_parser.py b/bin/report_modules/parsers/busco_parser.py index 22dcff27..74c6fb05 100644 --- a/bin/report_modules/parsers/busco_parser.py +++ b/bin/report_modules/parsers/busco_parser.py @@ -118,13 +118,14 @@ def get_deps_and_versions_dict(self, file_data): def get_busco_result_table(self, file_data): list_of_lines = file_data.split("\n") for index, line in enumerate(list_of_lines): - if "Dependencies and versions" in line: - dev_dep_index = index + if "Assembly Statistics" in line: + stats_index = index + break results_dict = {} for index, line in enumerate(list_of_lines): if "C:" in line: - for i in range(index + 1, dev_dep_index - 1): + for i in range(index + 1, stats_index - 1): number = list_of_lines[i].split("\t")[1] descr = list_of_lines[i].split("\t")[2] @@ -140,13 +141,13 @@ def get_busco_result_table(self, file_data): def get_busco_result_dict(self, file_data): list_of_lines = file_data.split("\n") for index, line in enumerate(list_of_lines): - if "Dependencies and versions" in line: - dev_dep_index = index + if "Assembly Statistics" in line: + stats_index = index results_dict = {} for index, line in enumerate(list_of_lines): if "C:" in line: - for i in range(index + 1, dev_dep_index - 1): + for i in range(index + 1, stats_index - 1): number = list_of_lines[i].split("\t")[1] descr = list_of_lines[i].split("\t")[2] diff --git a/conf/test_full.config b/conf/test_full.config index 31b136c8..4c4b5151 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -38,5 +38,5 @@ params { hic = 'SRR8238190' synteny_skip = false - synteny_xref_assemblies = 'tests/stub/xrefsheet.csv' + synteny_xref_assemblies = 'https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/xrefsheet.csv' } diff --git a/modules/local/busco.nf b/modules/local/busco.nf index c7538c8e..1c8b3f46 100644 --- a/modules/local/busco.nf +++ b/modules/local/busco.nf @@ -30,7 +30,6 @@ process BUSCO { -o ${asm_tag} \\ -i $fasta_file \\ -l ${lineage_dataset} \\ - --update-data \\ $lineages_path \\ -c ${task.cpus} diff --git a/modules/local/ncbi_fcs_adaptor.nf b/modules/local/ncbi_fcs_adaptor.nf index 63bed8e2..ec1b3dd0 100644 --- a/modules/local/ncbi_fcs_adaptor.nf +++ b/modules/local/ncbi_fcs_adaptor.nf @@ -4,8 +4,8 @@ process NCBI_FCS_ADAPTOR { // Warning: manually update version in script and stub container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://ftp.ncbi.nlm.nih.gov/genomes/TOOLS/FCS/releases/0.5.0/fcs-adaptor.sif': - 'biocontainers/ncbi-fcs-gx:0.5.0--h4ac6f70_3' }" + 'https://ftp.ncbi.nlm.nih.gov/genomes/TOOLS/FCS/releases/0.4.0/fcs-adaptor.sif': + 'docker.io/ncbi/fcs-adaptor:0.4.0' }" input: tuple val(asm_tag), path(fasta_file) @@ -23,7 +23,7 @@ process NCBI_FCS_ADAPTOR { if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { error "NCBI_FCS_ADAPTOR module does not support Conda. Please use Docker / Singularity / Podman instead." } - def VERSION = 0.5 + def VERSION = 0.4 """ mkdir "${asm_tag}_outputdir" @@ -46,7 +46,7 @@ process NCBI_FCS_ADAPTOR { if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { error "NCBI_FCS_ADAPTOR module does not support Conda. Please use Docker / Singularity / Podman instead." } - def VERSION = 0.5 + def VERSION = 0.4 """ touch "${asm_tag}_fcs_adaptor_report.tsv" diff --git a/nextflow.config b/nextflow.config index 795a3f41..c15d4da8 100644 --- a/nextflow.config +++ b/nextflow.config @@ -145,10 +145,11 @@ profiles { shifter.enabled = false charliecloud.enabled = false apptainer.enabled = false - docker.runOptions = '-u $(id -u):$(id -g)' + // docker.runOptions = '-u $(id -u):$(id -g)' DNADIFF fails } arm { - docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' + // docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' DNADIFF fails + docker.runOptions = '--platform=linux/amd64' } singularity { singularity.enabled = true From 8aefa66cf190ba0cf7db23f499e938a424d51651 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 28 Feb 2024 17:18:02 +1300 Subject: [PATCH 42/65] Added pfr profile and params --- pfr/params.json | 0 pfr/profile.config | 12 ++++++++++++ pfr_assemblyqc | 24 ++++++++++++++++++++++++ 3 files changed, 36 insertions(+) create mode 100644 pfr/params.json create mode 100644 pfr/profile.config create mode 100644 pfr_assemblyqc diff --git a/pfr/params.json b/pfr/params.json new file mode 100644 index 00000000..e69de29b diff --git a/pfr/profile.config b/pfr/profile.config new file mode 100644 index 00000000..f219d135 --- /dev/null +++ b/pfr/profile.config @@ -0,0 +1,12 @@ +profiles { + pfr { + process { + executor = 'slurm' + } + + apptainer { + envWhitelist = "APPTAINER_BINDPATH,APPTAINER_BIND" + cacheDir = "/workspace/assemblyqc/singularity" + } + } +} diff --git a/pfr_assemblyqc b/pfr_assemblyqc new file mode 100644 index 00000000..4f23a416 --- /dev/null +++ b/pfr_assemblyqc @@ -0,0 +1,24 @@ +#!/bin/bash -e + +#SBATCH --job-name ASMQC +#SBATCH --time=7-00:00:00 +#SBATCH --nodes=1 +#SBATCH --ntasks=1 +#SBATCH --cpus-per-task=1 +#SBATCH --output pfr_assemblyqc.stdout +#SBATCH --error pfr_assemblyqc.stderr +#SBATCH --mem=4G + +ml unload perl +ml apptainer/1.1 +ml nextflow/23.04.4 + +export TMPDIR="/workspace/$USER/tmp" +export APPTAINER_BINDPATH="$APPTAINER_BINDPATH,$TMPDIR:$TMPDIR,$TMPDIR:/tmp" + +nextflow \ + main.nf \ + -c pfr/profile.config \ + -profile pfr,apptainer \ + -resume \ + --params-file pfr/params.json From 1321d59307f511fccc351e642632754cc21c3d95 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 28 Feb 2024 19:30:38 +1300 Subject: [PATCH 43/65] Fixed a bug which caused lai to run with null assembly fasta --- workflows/assemblyqc.nf | 1 + 1 file changed, 1 insertion(+) diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index 17ca3f67..db45eb06 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -356,6 +356,7 @@ workflow ASSEMBLYQC { | map { meta, mono -> [ meta.id, mono ] }, remainder: true ) + | filter { id, fasta, mono -> fasta != null } | map { id, fasta, mono -> [ id, fasta, mono ?: [] ] } FASTA_LTRRETRIEVER_LAI( From cb325c087ffe1f91a7ed6ef461c5dc14cfa0729e Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 29 Feb 2024 09:55:01 +1300 Subject: [PATCH 44/65] Added a test for invalid files --- .github/workflows/ci.yml | 2 +- CHANGELOG.md | 3 +- bin/report_modules/templates/base.html | 119 +++++++++++-------------- modules/local/assembly2bedpe.nf | 2 +- modules/local/colourbundlelinks.nf | 2 +- modules/local/hic2html.nf | 2 +- modules/local/relabelbundlelinks.nf | 2 +- modules/local/relabelfastalength.nf | 2 +- tests/invalid/assemblysheet.csv | 4 + tests/invalid/invalid.fsa.gz | Bin 0 -> 39 bytes tests/invalid/params.json | 8 ++ 11 files changed, 74 insertions(+), 72 deletions(-) create mode 100644 tests/invalid/assemblysheet.csv create mode 100644 tests/invalid/invalid.fsa.gz create mode 100644 tests/invalid/params.json diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 3527c231..4ff4e1f8 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -25,10 +25,10 @@ jobs: matrix: NXF_VER: - "23.04.0" - - "latest-everything" RUN_CONFIG: - "-profile test,docker" - "-profile docker -c ./tests/stub/stub.config -stub" + - "-profile docker -params-file ./tests/invalid/params.json" steps: - name: Check out pipeline code uses: actions/checkout@v4 diff --git a/CHANGELOG.md b/CHANGELOG.md index 530a7213..bad08745 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v1.4dev - [20-Feb-2024] +## v1.4dev - [28-Feb-2024] ### `Added` @@ -24,6 +24,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 15. Refactored and cleaned data flows for all the custom sub-workflow 16. Started using nf-core template 17. Started using semantic versioning +18. Moved all python depending packages to 'docker.io/gallvp/python3npkgs:v0.6' ### `Fixed` diff --git a/bin/report_modules/templates/base.html b/bin/report_modules/templates/base.html index 8766284c..bb753a67 100644 --- a/bin/report_modules/templates/base.html +++ b/bin/report_modules/templates/base.html @@ -1,68 +1,57 @@ - {% include 'header.html' %} +{% include 'header.html' %} + + +
    +
    AssemblyQC {{ + all_stats_dicts['VERSIONS']['Workflow']['plant-food-research-open/assemblyqc'] }}
    +
    +
    + + + {% if 'FASTA_VALIDATE' in all_stats_dicts %} + + {% endif %} + {% if 'GFF3_VALIDATE' in all_stats_dicts %} + + {% endif %} + {% if 'NCBI_FCS_ADAPTOR' in all_stats_dicts %} + + {% endif %} {% if 'NCBI_FCS_GX' in all_stats_dicts %}{% endif %} {% if 'ASSEMBLATHON_STATS' in all_stats_dicts %}{% endif %} {% if 'GENOMETOOLS_GT_STAT' in all_stats_dicts %}{% endif %} {% if 'BUSCO' in all_stats_dicts %}{% endif %} {% if 'TIDK' in all_stats_dicts %}{% endif %} {% if 'LAI' in all_stats_dicts %}{% endif %} {% if 'KRAKEN2' in all_stats_dicts %}{% endif %} {% if 'HIC' in all_stats_dicts %}{% endif %} {% if 'CIRCOS' in all_stats_dicts %}{% endif %} +
    + {% include 'params/params.html' %} {% include 'tools/tools.html' %} {% if 'FASTA_VALIDATE' in all_stats_dicts %}{% + include 'fasta_validate/fasta_validate.html' %}{% endif %} + + {% if 'GFF3_VALIDATE' in all_stats_dicts %}{% include 'gff3_validate/gff3_validate.html' %}{% endif %} + + {% if 'NCBI_FCS_ADAPTOR' in all_stats_dicts %}{% + include 'ncbi_fcs_adaptor/ncbi_fcs_adaptor.html' %}{% endif %} {% if 'NCBI_FCS_GX' in all_stats_dicts %}{% + include 'ncbi_fcs_gx/ncbi_fcs_gx.html' %}{% endif %} {% if 'ASSEMBLATHON_STATS' in all_stats_dicts %}{% include + 'assemblathon_stats/assemblathon_stats.html' %}{% endif %} {% if 'GENOMETOOLS_GT_STAT' in all_stats_dicts %}{% + include 'genometools_gt_stat/genometools_gt_stat.html' %}{% endif %} {% if 'BUSCO' in all_stats_dicts %}{% + include 'busco/busco.html' %}{% endif %} {% if 'TIDK' in all_stats_dicts %}{% include 'tidk/tidk.html' %}{% + endif %} {% if 'LAI' in all_stats_dicts %}{% include 'lai/lai.html' %}{% endif %} {% if 'KRAKEN2' in + all_stats_dicts %}{% include 'kraken2/kraken2.html' %}{% endif %} {% if 'HIC' in all_stats_dicts %}{% include + 'hic/hic.html' %}{% endif %} {% if 'CIRCOS' in all_stats_dicts %}{% include 'circos/circos.html' %}{% endif %} + +{% include 'js.html' %} - -
    -
    AssemblyQC {{ all_stats_dicts['VERSIONS']['Workflow']['plant-food-research-open/assemblyqc'] }}
    -
    -
    - - - {% if 'GFF3_VALIDATE' in all_stats_dicts %} - - {% endif %} {% if 'FASTA_VALIDATE' in all_stats_dicts %} - - {% endif %} {% if 'NCBI_FCS_ADAPTOR' in all_stats_dicts %} - - {% endif %} {% if 'NCBI_FCS_GX' in all_stats_dicts %}{% endif %} {% if 'ASSEMBLATHON_STATS' in all_stats_dicts %}{% endif %} {% if 'GENOMETOOLS_GT_STAT' in all_stats_dicts %}{% endif %} {% if 'BUSCO' in all_stats_dicts %}{% endif %} {% if 'TIDK' in all_stats_dicts %}{% endif %} {% if 'LAI' in all_stats_dicts %}{% endif %} {% if 'KRAKEN2' in all_stats_dicts %}{% endif %} {% if 'HIC' in all_stats_dicts %}{% endif %} {% if 'CIRCOS' in all_stats_dicts %}{% endif %} -
    - {% include 'params/params.html' %} {% include 'tools/tools.html' %} {% if 'GFF3_VALIDATE' in all_stats_dicts - %}{% include 'gff3_validate/gff3_validate.html' %}{% endif %} {% if 'FASTA_VALIDATE' in all_stats_dicts %}{% - include 'fasta_validate/fasta_validate.html' %}{% endif %} {% if 'NCBI_FCS_ADAPTOR' in all_stats_dicts %}{% - include 'ncbi_fcs_adaptor/ncbi_fcs_adaptor.html' %}{% endif %} {% if 'NCBI_FCS_GX' in all_stats_dicts %}{% - include 'ncbi_fcs_gx/ncbi_fcs_gx.html' %}{% endif %} {% if 'ASSEMBLATHON_STATS' in all_stats_dicts %}{% include - 'assemblathon_stats/assemblathon_stats.html' %}{% endif %} {% if 'GENOMETOOLS_GT_STAT' in all_stats_dicts %}{% - include 'genometools_gt_stat/genometools_gt_stat.html' %}{% endif %} {% if 'BUSCO' in all_stats_dicts %}{% - include 'busco/busco.html' %}{% endif %} {% if 'TIDK' in all_stats_dicts %}{% include 'tidk/tidk.html' %}{% - endif %} {% if 'LAI' in all_stats_dicts %}{% include 'lai/lai.html' %}{% endif %} {% if 'KRAKEN2' in - all_stats_dicts %}{% include 'kraken2/kraken2.html' %}{% endif %} {% if 'HIC' in all_stats_dicts %}{% include - 'hic/hic.html' %}{% endif %} {% if 'CIRCOS' in all_stats_dicts %}{% include 'circos/circos.html' %}{% endif %} - - {% include 'js.html' %} diff --git a/modules/local/assembly2bedpe.nf b/modules/local/assembly2bedpe.nf index ef6cebd7..48e99f6c 100644 --- a/modules/local/assembly2bedpe.nf +++ b/modules/local/assembly2bedpe.nf @@ -2,7 +2,7 @@ process ASSEMBLY2BEDPE { tag "$sample_id_on_tag" label 'process_single' - container "docker.io/gallvp/python3npkgs:v0.4" + container "docker.io/gallvp/python3npkgs:v0.6" input: tuple val(sample_id_on_tag), path(agp_assembly_file) diff --git a/modules/local/colourbundlelinks.nf b/modules/local/colourbundlelinks.nf index 125a747f..6d753984 100644 --- a/modules/local/colourbundlelinks.nf +++ b/modules/local/colourbundlelinks.nf @@ -2,7 +2,7 @@ process COLOURBUNDLELINKS { tag "${target_on_ref}" label 'process_single' - container "docker.io/gallvp/python3npkgs:v0.4" + container "docker.io/gallvp/python3npkgs:v0.6" input: tuple val(target_on_ref), path(bundle_links) diff --git a/modules/local/hic2html.nf b/modules/local/hic2html.nf index 6149cdfd..0b907cde 100644 --- a/modules/local/hic2html.nf +++ b/modules/local/hic2html.nf @@ -2,7 +2,7 @@ process HIC2HTML { tag "$sample_id_on_tag" label 'process_single' - container "docker.io/gallvp/python3npkgs:v0.4" + container "docker.io/gallvp/python3npkgs:v0.6" input: tuple val(sample_id_on_tag), path(hic_file) diff --git a/modules/local/relabelbundlelinks.nf b/modules/local/relabelbundlelinks.nf index 99bb27e7..0b95fb05 100644 --- a/modules/local/relabelbundlelinks.nf +++ b/modules/local/relabelbundlelinks.nf @@ -2,7 +2,7 @@ process RELABELBUNDLELINKS { tag "${target_on_ref}" label 'process_single' - container "docker.io/gallvp/python3npkgs:v0.4" + container "docker.io/gallvp/python3npkgs:v0.6" input: tuple val(target_on_ref), path(coloured_bundle_links), path(target_seq_list), path(ref_seq_list) diff --git a/modules/local/relabelfastalength.nf b/modules/local/relabelfastalength.nf index 74abe98e..6c9615ab 100644 --- a/modules/local/relabelfastalength.nf +++ b/modules/local/relabelfastalength.nf @@ -2,7 +2,7 @@ process RELABELFASTALENGTH { tag "${target_on_ref}" label 'process_single' - container "docker.io/gallvp/python3npkgs:v0.4" + container "docker.io/gallvp/python3npkgs:v0.6" input: tuple val(target_on_ref), path(target_seq_lengths), path(ref_seq_lengths), path(target_seq_list), path(ref_seq_list) diff --git a/tests/invalid/assemblysheet.csv b/tests/invalid/assemblysheet.csv new file mode 100644 index 00000000..de7e586f --- /dev/null +++ b/tests/invalid/assemblysheet.csv @@ -0,0 +1,4 @@ +tag,fasta,gff3,monoploid_ids,synteny_labels +FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz,, +TT_2021a,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/021/950/295/GCA_021950295.1_ASM2195029v1/GCA_021950295.1_ASM2195029v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz,, +MISC,tests/invalid/invalid.fsa.gz,,, diff --git a/tests/invalid/invalid.fsa.gz b/tests/invalid/invalid.fsa.gz new file mode 100644 index 0000000000000000000000000000000000000000..b60e96868b3d6c1c80326e912fb85ed7dd5633ca GIT binary patch literal 39 vcmb2|=HPhn=UyrUb7o#yVoqj?URrS?LvaaTVY$oU7=}xKW;%*8FfafBD_ssL literal 0 HcmV?d00001 diff --git a/tests/invalid/params.json b/tests/invalid/params.json new file mode 100644 index 00000000..dd017c16 --- /dev/null +++ b/tests/invalid/params.json @@ -0,0 +1,8 @@ +{ + "config_profile_name": "Invalid profile", + "config_profile_description": "Profile to test invalid files", + "input": "tests/invalid/assemblysheet.csv", + "max_cpus": 2, + "max_memory": "6.GB", + "max_time": "6.h" +} From 39f6854e2dbf0077882d58d8cf4822532a12e956 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 29 Feb 2024 10:08:28 +1300 Subject: [PATCH 45/65] Updated modules and subworkflows --- modules.json | 12 +- modules/pfr/bwa/index/tests/main.nf.test | 2 +- modules/pfr/bwa/mem/tests/main.nf.test | 20 +-- modules/pfr/cat/cat/tests/main.nf.test | 29 ++-- modules/pfr/cat/cat/tests/main.nf.test.snap | 92 ++++------- modules/pfr/ltrharvest/meta.yml | 13 +- modules/pfr/samblaster/environment.yml | 4 +- modules/pfr/samblaster/meta.yml | 1 + modules/pfr/samblaster/tests/main.nf.test | 57 +++++++ .../pfr/samblaster/tests/main.nf.test.snap | 72 +++++++++ modules/pfr/samblaster/tests/nextflow.config | 6 + modules/pfr/samblaster/tests/tags.yml | 2 + nextflow.config | 5 +- .../pfr/fasta_ltrretriever_lai/main.nf | 150 +++++++++++------- .../fasta_ltrretriever_lai/tests/main.nf.test | 107 ++++++++++++- 15 files changed, 414 insertions(+), 158 deletions(-) create mode 100644 modules/pfr/samblaster/tests/main.nf.test create mode 100644 modules/pfr/samblaster/tests/main.nf.test.snap create mode 100644 modules/pfr/samblaster/tests/nextflow.config create mode 100644 modules/pfr/samblaster/tests/tags.yml diff --git a/modules.json b/modules.json index a57849ee..47c20082 100644 --- a/modules.json +++ b/modules.json @@ -7,17 +7,17 @@ "pfr": { "bwa/index": { "branch": "main", - "git_sha": "4b9da80b1e4c16067babd97554bea42d7cd9ca85", + "git_sha": "6410ddc6dfcee5ed480f47199cbd527662cdf5fe", "installed_by": ["fastq_bwa_mem_samblaster"] }, "bwa/mem": { "branch": "main", - "git_sha": "835879b8f174bb4d2c5534d0381ffbe62cc1b060", + "git_sha": "6410ddc6dfcee5ed480f47199cbd527662cdf5fe", "installed_by": ["fastq_bwa_mem_samblaster"] }, "cat/cat": { "branch": "main", - "git_sha": "4b9da80b1e4c16067babd97554bea42d7cd9ca85", + "git_sha": "6410ddc6dfcee5ed480f47199cbd527662cdf5fe", "installed_by": ["fasta_ltrretriever_lai"] }, "custom/checkgff3fastacorrespondence": { @@ -57,7 +57,7 @@ }, "ltrharvest": { "branch": "main", - "git_sha": "835879b8f174bb4d2c5534d0381ffbe62cc1b060", + "git_sha": "6410ddc6dfcee5ed480f47199cbd527662cdf5fe", "installed_by": ["fasta_ltrretriever_lai"] }, "ltrretriever/lai": { @@ -72,7 +72,7 @@ }, "samblaster": { "branch": "main", - "git_sha": "73358a6712178b9a67c39f92e65e8144b5880eae", + "git_sha": "6410ddc6dfcee5ed480f47199cbd527662cdf5fe", "installed_by": ["fastq_bwa_mem_samblaster"] } } @@ -81,7 +81,7 @@ "pfr": { "fasta_ltrretriever_lai": { "branch": "main", - "git_sha": "154661d7c1769532ff7b5f11259644ec200dd47d", + "git_sha": "60ee50c79f8f868fe0b2e48cba543ad1d8fa89b2", "installed_by": ["subworkflows"] }, "fastq_bwa_mem_samblaster": { diff --git a/modules/pfr/bwa/index/tests/main.nf.test b/modules/pfr/bwa/index/tests/main.nf.test index 2f33c0e8..af33e73c 100644 --- a/modules/pfr/bwa/index/tests/main.nf.test +++ b/modules/pfr/bwa/index/tests/main.nf.test @@ -15,7 +15,7 @@ nextflow_process { """ input[0] = [ [id: 'test'], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] """ } diff --git a/modules/pfr/bwa/mem/tests/main.nf.test b/modules/pfr/bwa/mem/tests/main.nf.test index cd6591ff..2696e4bf 100644 --- a/modules/pfr/bwa/mem/tests/main.nf.test +++ b/modules/pfr/bwa/mem/tests/main.nf.test @@ -18,7 +18,7 @@ nextflow_process { """ input[0] = [ [id: 'test'], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] """ } @@ -31,7 +31,7 @@ nextflow_process { input[0] = [ [ id:'test', single_end:true ], // meta map [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] ] input[1] = BWA_INDEX.out.index @@ -58,7 +58,7 @@ nextflow_process { """ input[0] = [ [id: 'test'], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] """ } @@ -71,7 +71,7 @@ nextflow_process { input[0] = [ [ id:'test', single_end:true ], // meta map [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] ] input[1] = BWA_INDEX.out.index @@ -98,7 +98,7 @@ nextflow_process { """ input[0] = [ [id: 'test'], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] """ } @@ -111,8 +111,8 @@ nextflow_process { input[0] = [ [ id:'test', single_end:false ], // meta map [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] ] input[1] = BWA_INDEX.out.index @@ -139,7 +139,7 @@ nextflow_process { """ input[0] = [ [id: 'test'], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] """ } @@ -152,8 +152,8 @@ nextflow_process { input[0] = [ [ id:'test', single_end:false ], // meta map [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] ] input[1] = BWA_INDEX.out.index diff --git a/modules/pfr/cat/cat/tests/main.nf.test b/modules/pfr/cat/cat/tests/main.nf.test index aaae04f9..fcee2d19 100644 --- a/modules/pfr/cat/cat/tests/main.nf.test +++ b/modules/pfr/cat/cat/tests/main.nf.test @@ -19,8 +19,8 @@ nextflow_process { [ [ id:'genome', single_end:true ], [ - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), - file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.sizes', checkIfExists: true) ] ] """ @@ -45,8 +45,8 @@ nextflow_process { [ [ id:'test', single_end:true ], [ - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), - file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.sizes', checkIfExists: true) ] ] """ @@ -72,8 +72,8 @@ nextflow_process { [ [ id:'test', single_end:true ], [ - file(params.test_data['sarscov2']['genome']['genome_gff3_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['genome']['contigs_genome_maf_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.gff3.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/alignment/last/contigs.genome.maf.gz', checkIfExists: true) ] ] """ @@ -83,7 +83,8 @@ nextflow_process { def lines = path(process.out.file_out.get(0).get(1)).linesGzip assertAll( { assert process.success }, - { assert snapshot(process.out).match() } + { assert snapshot(lines[0..5]).match("test_cat_zipped_zipped_lines") }, + { assert snapshot(lines.size()).match("test_cat_zipped_zipped_size")} ) } } @@ -101,8 +102,8 @@ nextflow_process { [ [ id:'test', single_end:true ], [ - file(params.test_data['sarscov2']['genome']['genome_gff3_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['genome']['contigs_genome_maf_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.gff3.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/alignment/last/contigs.genome.maf.gz', checkIfExists: true) ] ] """ @@ -130,8 +131,8 @@ nextflow_process { [ [ id:'test', single_end:true ], [ - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), - file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.sizes', checkIfExists: true) ] ] """ @@ -141,7 +142,8 @@ nextflow_process { def lines = path(process.out.file_out.get(0).get(1)).linesGzip assertAll( { assert process.success }, - { assert snapshot(process.out).match() } + { assert snapshot(lines[0..5]).match("test_cat_unzipped_zipped_lines") }, + { assert snapshot(lines.size()).match("test_cat_unzipped_zipped_size")} ) } } @@ -158,7 +160,7 @@ nextflow_process { [ [ id:'test', single_end:true ], [ - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] ] """ @@ -174,4 +176,3 @@ nextflow_process { } } } - diff --git a/modules/pfr/cat/cat/tests/main.nf.test.snap b/modules/pfr/cat/cat/tests/main.nf.test.snap index 0c9bfe8d..423571ba 100644 --- a/modules/pfr/cat/cat/tests/main.nf.test.snap +++ b/modules/pfr/cat/cat/tests/main.nf.test.snap @@ -1,4 +1,10 @@ { + "test_cat_unzipped_zipped_size": { + "content": [ + 375 + ], + "timestamp": "2023-10-16T14:33:08.049445686" + }, "test_cat_unzipped_unzipped": { "content": [ { @@ -61,36 +67,31 @@ ], "timestamp": "2023-10-16T14:32:49.642741302" }, - "test_cat_zipped_zipped": { + "test_cat_zipped_zipped_lines": { "content": [ - { - "0": [ - [ - { - "id": "test", - "single_end": true - }, - "test.gff3.gz:md5,c439d3b60e7bc03e8802a451a0d9a5d9" - ] - ], - "1": [ - "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" - ], - "file_out": [ - [ - { - "id": "test", - "single_end": true - }, - "test.gff3.gz:md5,c439d3b60e7bc03e8802a451a0d9a5d9" - ] - ], - "versions": [ - "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" - ] - } + [ + "MT192765.1\tGenbank\ttranscript\t259\t29667\t.\t+\t.\tID=unknown_transcript_1;geneID=orf1ab;gene_name=orf1ab", + "MT192765.1\tGenbank\tgene\t259\t21548\t.\t+\t.\tParent=unknown_transcript_1", + "MT192765.1\tGenbank\tCDS\t259\t13461\t.\t+\t0\tParent=unknown_transcript_1;exception=\"ribosomal slippage\";gbkey=CDS;gene=orf1ab;note=\"pp1ab;translated=by -1 ribosomal frameshift\";product=\"orf1ab polyprotein\";protein_id=QIK50426.1", + "MT192765.1\tGenbank\tCDS\t13461\t21548\t.\t+\t0\tParent=unknown_transcript_1;exception=\"ribosomal slippage\";gbkey=CDS;gene=orf1ab;note=\"pp1ab;translated=by -1 ribosomal frameshift\";product=\"orf1ab polyprotein\";protein_id=QIK50426.1", + "MT192765.1\tGenbank\tCDS\t21556\t25377\t.\t+\t0\tParent=unknown_transcript_1;gbkey=CDS;gene=S;note=\"structural protein\";product=\"surface glycoprotein\";protein_id=QIK50427.1", + "MT192765.1\tGenbank\tgene\t21556\t25377\t.\t+\t.\tParent=unknown_transcript_1" + ] + ], + "timestamp": "2023-10-16T14:32:33.629048645" + }, + "test_cat_unzipped_zipped_lines": { + "content": [ + [ + ">MT192765.1 Severe acute respiratory syndrome coronavirus 2 isolate SARS-CoV-2/human/USA/PC00101P/2020, complete genome", + "GTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGT", + "GTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAG", + "TAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGG", + "GTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTT", + "ACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAG" + ] ], - "timestamp": "2024-01-12T14:02:02.999254641" + "timestamp": "2023-10-16T14:33:08.038830506" }, "test_cat_one_file_unzipped_zipped_lines": { "content": [ @@ -105,41 +106,16 @@ ], "timestamp": "2023-10-16T14:33:21.39642399" }, - "test_cat_unzipped_zipped": { + "test_cat_zipped_zipped_size": { "content": [ - { - "0": [ - [ - { - "id": "test", - "single_end": true - }, - "cat.txt.gz:md5,f44b33a0e441ad58b2d3700270e2dbe2" - ] - ], - "1": [ - "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" - ], - "file_out": [ - [ - { - "id": "test", - "single_end": true - }, - "cat.txt.gz:md5,f44b33a0e441ad58b2d3700270e2dbe2" - ] - ], - "versions": [ - "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894" - ] - } + 78 ], - "timestamp": "2024-01-12T14:08:26.948048418" + "timestamp": "2023-10-16T14:32:33.641869244" }, "test_cat_one_file_unzipped_zipped_size": { "content": [ 374 ], - "timestamp": "2024-01-12T14:10:22.445700266" + "timestamp": "2023-10-16T14:33:21.4094373" } -} +} \ No newline at end of file diff --git a/modules/pfr/ltrharvest/meta.yml b/modules/pfr/ltrharvest/meta.yml index efda0670..256b3ce5 100644 --- a/modules/pfr/ltrharvest/meta.yml +++ b/modules/pfr/ltrharvest/meta.yml @@ -12,13 +12,12 @@ keywords: - transposons - retrotransposons tools: - - "edta": - description: Extensive de-novo TE Annotator (EDTA) - homepage: "https://github.com/oushujun/EDTA" - documentation: "https://github.com/oushujun/EDTA" - tool_dev_url: "https://github.com/oushujun/EDTA" - doi: "10.1186/s13059-019-1905-y" - licence: ["GPL v3"] + - "LTR_HARVEST_parallel": + description: A Perl wrapper for LTR_harvest + homepage: "https://github.com/oushujun/EDTA/tree/v2.2.0/bin/LTR_HARVEST_parallel" + documentation: "https://github.com/oushujun/EDTA/tree/v2.2.0/bin/LTR_HARVEST_parallel" + tool_dev_url: "https://github.com/oushujun/EDTA/tree/v2.2.0/bin/LTR_HARVEST_parallel" + licence: ["MIT"] - "gt": description: "The GenomeTools genome analysis system" homepage: "https://genometools.org/index.html" diff --git a/modules/pfr/samblaster/environment.yml b/modules/pfr/samblaster/environment.yml index a6e3038a..ac838241 100644 --- a/modules/pfr/samblaster/environment.yml +++ b/modules/pfr/samblaster/environment.yml @@ -1,9 +1,11 @@ name: samblaster + channels: - conda-forge - bioconda - defaults + dependencies: + - bioconda::htslib=1.19.1 - bioconda::samblaster=0.1.26 - bioconda::samtools=1.19.2 - - bioconda::htslib=1.19.1 diff --git a/modules/pfr/samblaster/meta.yml b/modules/pfr/samblaster/meta.yml index ccb48320..5c1e5a97 100644 --- a/modules/pfr/samblaster/meta.yml +++ b/modules/pfr/samblaster/meta.yml @@ -51,3 +51,4 @@ authors: - "@lescai" maintainers: - "@lescai" + - "@gallvp" diff --git a/modules/pfr/samblaster/tests/main.nf.test b/modules/pfr/samblaster/tests/main.nf.test new file mode 100644 index 00000000..01794307 --- /dev/null +++ b/modules/pfr/samblaster/tests/main.nf.test @@ -0,0 +1,57 @@ +nextflow_process { + + name "Test Process SAMBLASTER" + script "../main.nf" + process "SAMBLASTER" + + tag "modules" + tag "modules_nfcore" + tag "samblaster" + + test("homo_sapiens-test_paired_end_umi_unsorted_bam") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_umi_unsorted_bam'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_umi_unsorted_bam'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/pfr/samblaster/tests/main.nf.test.snap b/modules/pfr/samblaster/tests/main.nf.test.snap new file mode 100644 index 00000000..917c8f1f --- /dev/null +++ b/modules/pfr/samblaster/tests/main.nf.test.snap @@ -0,0 +1,72 @@ +{ + "stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + "versions.yml:md5,8a70467f2dfc2e0d8e81787223d2fc77" + ], + "bam": [ + [ + { + "id": "test", + "single_end": false + }, + "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,8a70467f2dfc2e0d8e81787223d2fc77" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-26T14:04:42.510824" + }, + "homo_sapiens-test_paired_end_umi_unsorted_bam": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.bam:md5,634a6bd541478e970f0a4c279f399889" + ] + ], + "1": [ + "versions.yml:md5,8a70467f2dfc2e0d8e81787223d2fc77" + ], + "bam": [ + [ + { + "id": "test", + "single_end": false + }, + "test.bam:md5,634a6bd541478e970f0a4c279f399889" + ] + ], + "versions": [ + "versions.yml:md5,8a70467f2dfc2e0d8e81787223d2fc77" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-26T14:04:38.118875" + } +} \ No newline at end of file diff --git a/modules/pfr/samblaster/tests/nextflow.config b/modules/pfr/samblaster/tests/nextflow.config new file mode 100644 index 00000000..605e74eb --- /dev/null +++ b/modules/pfr/samblaster/tests/nextflow.config @@ -0,0 +1,6 @@ +process { + withName: SAMBLASTER { + ext.args = '-M --addMateTags' + ext.prefix = { "${meta.id}.processed" } + } +} diff --git a/modules/pfr/samblaster/tests/tags.yml b/modules/pfr/samblaster/tests/tags.yml new file mode 100644 index 00000000..3882ee54 --- /dev/null +++ b/modules/pfr/samblaster/tests/tags.yml @@ -0,0 +1,2 @@ +samblaster: + - "modules/nf-core/samblaster/**" diff --git a/nextflow.config b/nextflow.config index c15d4da8..795a3f41 100644 --- a/nextflow.config +++ b/nextflow.config @@ -145,11 +145,10 @@ profiles { shifter.enabled = false charliecloud.enabled = false apptainer.enabled = false - // docker.runOptions = '-u $(id -u):$(id -g)' DNADIFF fails + docker.runOptions = '-u $(id -u):$(id -g)' } arm { - // docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' DNADIFF fails - docker.runOptions = '--platform=linux/amd64' + docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' } singularity { singularity.enabled = true diff --git a/subworkflows/pfr/fasta_ltrretriever_lai/main.nf b/subworkflows/pfr/fasta_ltrretriever_lai/main.nf index 566e6821..ea5183b1 100644 --- a/subworkflows/pfr/fasta_ltrretriever_lai/main.nf +++ b/subworkflows/pfr/fasta_ltrretriever_lai/main.nf @@ -9,50 +9,59 @@ include { CUSTOM_RESTOREGFFIDS } from '../../../modules/pfr/custom/restoreg workflow FASTA_LTRRETRIEVER_LAI { take: - ch_fasta // channel: [ val(meta), fasta ] - ch_monoploid_seqs // channel: [ val(meta), txt ]; Optional: Set to [] if not needed - skip_lai // val; true|false + ch_fasta // channel: [ val(meta), fasta ] + ch_monoploid_seqs // channel: [ val(meta), txt ]; Optional: Set to [] if not needed + // val(meta) from ch_fasta and ch_monoploid_seqs are only required + // to have the same `id` + skip_lai // val(true|false) main: - - ch_versions = Channel.empty() + ch_versions = Channel.empty() // MOUDLE: CUSTOM_SHORTENFASTAIDS CUSTOM_SHORTENFASTAIDS ( ch_fasta ) - ch_short_ids_fasta = ch_fasta - | join(CUSTOM_SHORTENFASTAIDS.out.short_ids_fasta, by:0, remainder:true) - | map { meta, fasta, short_ids_fasta -> - [ meta, short_ids_fasta ?: fasta ] - } - - ch_short_ids_tsv = CUSTOM_SHORTENFASTAIDS.out.short_ids_tsv - ch_versions = ch_versions.mix(CUSTOM_SHORTENFASTAIDS.out.versions.first()) + ch_short_ids_fasta = ch_fasta + | join(CUSTOM_SHORTENFASTAIDS.out.short_ids_fasta, by:0, remainder:true) + | map { meta, fasta, short_ids_fasta -> + [ meta, short_ids_fasta ?: fasta ] + } + + ch_short_ids_tsv = CUSTOM_SHORTENFASTAIDS.out.short_ids_tsv + ch_short_monoploid_seqs = ch_short_ids_tsv + | join( + ch_monoploid_seqs ?: Channel.empty() + ) + | map { meta, short_ids_tsv, monoploid_seqs -> + map_monoploid_seqs_to_new_ids(meta, short_ids_tsv, monoploid_seqs) + } + ch_versions = ch_versions.mix(CUSTOM_SHORTENFASTAIDS.out.versions.first()) // MODULE: LTRHARVEST LTRHARVEST ( ch_short_ids_fasta ) - ch_ltrharvest_scn = LTRHARVEST.out.scn - ch_versions = ch_versions.mix(LTRHARVEST.out.versions.first()) + ch_ltrharvest_scn = LTRHARVEST.out.scn + ch_versions = ch_versions.mix(LTRHARVEST.out.versions.first()) // MODULE: LTRFINDER LTRFINDER ( ch_short_ids_fasta ) - ch_ltrfinder_scn = LTRFINDER.out.scn - ch_versions = ch_versions.mix(LTRFINDER.out.versions.first()) + ch_ltrfinder_scn = LTRFINDER.out.scn + ch_versions = ch_versions.mix(LTRFINDER.out.versions.first()) // MODULE: CAT_CAT - ch_cat_cat_inputs = ch_ltrharvest_scn - | join(ch_ltrfinder_scn) - | map { meta, harvested, found -> [ meta, [ harvested, found ] ] } + ch_cat_cat_inputs = ch_ltrharvest_scn + | join(ch_ltrfinder_scn) + | map { meta, harvested, found -> [ meta, [ harvested, found ] ] } CAT_CAT ( ch_cat_cat_inputs ) - ch_ltr_candidates = CAT_CAT.out.file_out - ch_versions = ch_versions.mix(CAT_CAT.out.versions.first()) + ch_ltr_candidates = CAT_CAT.out.file_out + ch_versions = ch_versions.mix(CAT_CAT.out.versions.first()) // MODULE: LTRRETRIEVER_LTRRETRIEVER - ch_ltrretriever_inputs = ch_short_ids_fasta.join(ch_ltr_candidates) + ch_ltrretriever_inputs = ch_short_ids_fasta.join(ch_ltr_candidates) + LTRRETRIEVER_LTRRETRIEVER ( ch_ltrretriever_inputs.map { meta, fasta, ltr -> [ meta, fasta ] }, ch_ltrretriever_inputs.map { meta, fasta, ltr -> ltr }, @@ -61,26 +70,30 @@ workflow FASTA_LTRRETRIEVER_LAI { [] ) - ch_pass_list = LTRRETRIEVER_LTRRETRIEVER.out.pass_list - ch_ltrlib = LTRRETRIEVER_LTRRETRIEVER.out.ltrlib - ch_annotation_out = LTRRETRIEVER_LTRRETRIEVER.out.annotation_out - ch_annotation_gff = LTRRETRIEVER_LTRRETRIEVER.out.annotation_gff - ch_versions = ch_versions.mix(LTRRETRIEVER_LTRRETRIEVER.out.versions.first()) + ch_pass_list = LTRRETRIEVER_LTRRETRIEVER.out.pass_list + ch_ltrlib = LTRRETRIEVER_LTRRETRIEVER.out.ltrlib + ch_annotation_out = LTRRETRIEVER_LTRRETRIEVER.out.annotation_out + ch_annotation_gff = LTRRETRIEVER_LTRRETRIEVER.out.annotation_gff + ch_versions = ch_versions.mix(LTRRETRIEVER_LTRRETRIEVER.out.versions.first()) // MODULE: LAI - ch_lai_inputs = skip_lai - ? Channel.empty() - : ch_short_ids_fasta - | join(ch_pass_list) - | join(ch_annotation_out) - | join( - ch_monoploid_seqs ?: Channel.empty(), - by:0, - remainder: true - ) - | map { meta, fasta, pass, out, mono -> - [ meta, fasta, pass, out, mono ?: [] ] - } + ch_lai_inputs = skip_lai + ? Channel.empty() + : ch_short_ids_fasta + | join(ch_pass_list) + | join(ch_annotation_out) + | map { meta, fasta, pass, out -> + [ meta.id, meta, fasta, pass, out ] + } + | join( + ch_short_monoploid_seqs + | map { meta, mono -> [ meta.id, mono ] }, + by:0, + remainder: true + ) + | map { id, meta, fasta, pass, out, mono -> + [ meta, fasta, pass, out, mono ?: [] ] + } LTRRETRIEVER_LAI( ch_lai_inputs.map { meta, fasta, pass, out, mono -> [ meta, fasta ] }, ch_lai_inputs.map { meta, fasta, pass, out, mono -> pass }, @@ -88,27 +101,56 @@ workflow FASTA_LTRRETRIEVER_LAI { ch_lai_inputs.map { meta, fasta, pass, out, mono -> mono } ) - ch_lai_log = LTRRETRIEVER_LAI.out.log - ch_lai_out = LTRRETRIEVER_LAI.out.lai_out - ch_versions = ch_versions.mix(LTRRETRIEVER_LAI.out.versions.first()) + ch_lai_log = LTRRETRIEVER_LAI.out.log + ch_lai_out = LTRRETRIEVER_LAI.out.lai_out + ch_versions = ch_versions.mix(LTRRETRIEVER_LAI.out.versions.first()) // MODULE: CUSTOM_RESTOREGFFIDS - ch_restorable_gff_tsv = ch_annotation_gff.join(ch_short_ids_tsv) + ch_restorable_gff_tsv = ch_annotation_gff.join(ch_short_ids_tsv) CUSTOM_RESTOREGFFIDS ( ch_restorable_gff_tsv.map { meta, gff, tsv -> [ meta, gff ] }, ch_restorable_gff_tsv.map { meta, gff, tsv -> tsv } ) - ch_restored_gff = ch_annotation_gff - | join(CUSTOM_RESTOREGFFIDS.out.restored_ids_gff3, by:0, remainder:true) - | map { meta, gff, restored_gff -> [ meta, restored_gff ?: gff ] } - ch_versions = ch_versions.mix(CUSTOM_RESTOREGFFIDS.out.versions.first()) + ch_restored_gff = ch_annotation_gff + | join(CUSTOM_RESTOREGFFIDS.out.restored_ids_gff3, by:0, remainder:true) + | map { meta, gff, restored_gff -> [ meta, restored_gff ?: gff ] } + + ch_versions = ch_versions.mix(CUSTOM_RESTOREGFFIDS.out.versions.first()) emit: - ltrlib = ch_ltrlib // channel: [ val(meta), fasta ] - annotation_gff = ch_restored_gff // channel: [ val(meta), gff ] - lai_log = ch_lai_log // channel: [ val(meta), log ] - lai_out = ch_lai_out // channel: [ val(meta), out ] - versions = ch_versions // channel: [ versions.yml ] + ltrlib = ch_ltrlib // channel: [ val(meta), fasta ] + annotation_gff = ch_restored_gff // channel: [ val(meta), gff ] + lai_log = ch_lai_log // channel: [ val(meta), log ] + lai_out = ch_lai_out // channel: [ val(meta), out ] + versions = ch_versions // channel: [ versions.yml ] +} + + +def map_monoploid_seqs_to_new_ids(meta, short_ids_tsv, monoploid_seqs) { + + def short_ids_head = short_ids_tsv.text.split('\n')[0] + + if (short_ids_head == "IDs have acceptable length and character. No change required.") { + return [ meta, monoploid_seqs ] + } + + def orig_to_new_ids = [:] + short_ids_tsv.text.eachLine { line -> + def (original_id, renamed_id) = line.split('\t') + orig_to_new_ids[original_id] = renamed_id + } + + def output_file = new File("${meta.id}.mapped.monoploid.seqs.txt") + monoploid_seqs.text.eachLine { original_id -> + if (!orig_to_new_ids[original_id]) { + error "Faild to find $original_id in ${monoploid_seqs}" + + "The monoploid_seqs file is malformed!" + } + + output_file.append(orig_to_new_ids[original_id]) + } + + return [ meta, output_file.toPath() ] } diff --git a/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test b/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test index 773c514a..f2c401d8 100644 --- a/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test +++ b/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test @@ -18,7 +18,7 @@ nextflow_workflow { tag "ltrretriever/lai" tag "custom/restoregffids" - test("actinidia_chinensis-genome_21_fasta_gz-success") { + test("actinidia_chinensis-genome_21_fasta_gz") { setup { run("GUNZIP") { @@ -38,9 +38,9 @@ nextflow_workflow { when { workflow { """ - input[0] = GUNZIP.out.gunzip - input[1] = [] - input[2] = false + input[0] = GUNZIP.out.gunzip + input[1] = [] + input[2] = false """ } } @@ -56,4 +56,103 @@ nextflow_workflow { ) } } + + test("actinidia_chinensis-genome_21_fasta_gz-with_mono") { + + setup { + run("GUNZIP") { + script "../../../../modules/nf-core/gunzip" + + process { + """ + input[0] = [ + [ id:'test' ], + file(params.test_data['actinidia_chinensis']['genome']['genome_21_fasta_gz'], checkIfExists: true) + ] + """ + } + } + } + + when { + workflow { + """ + GUNZIP.out.gunzip.map { meta, fasta -> + def fa = new File('test.fa') + fa.write(fasta.text.replaceAll('>chr1', '>chr_xxxxxxxxxxxxxxx_1')) + + [ meta, fa.toPath() ] + } + | set { ch_fa } + + def monoploid_seqs = new File('test.mono.seq.txt') + monoploid_seqs.write("chr_xxxxxxxxxxxxxxx_1") + + input[0] = ch_fa + input[1] = Channel.of( [ [ id:'test' ], monoploid_seqs.toPath() ] ) + input[2] = false + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert file(workflow.out.annotation_gff[0][1]).text.contains('Copia_LTR_retrotransposon') }, + { assert file(workflow.out.lai_log[0][1]).text.contains('Calculate LAI:') }, + { assert file(workflow.out.lai_log[0][1]).text.contains('Done!') }, + { assert Math.abs(Float.parseFloat(path(workflow.out.lai_out[0][1]).text.split("\n")[1].split("\t")[6]) - 31.29) <= 1.0 }, + { assert file(workflow.out.ltrlib[0][1]).text.contains('#LTR/Copia') } + ) + } + } + + test("actinidia_chinensis-genome_21_fasta_gz-without_lai") { + + setup { + run("GUNZIP") { + script "../../../../modules/nf-core/gunzip" + + process { + """ + input[0] = [ + [ id:'test' ], + file(params.test_data['actinidia_chinensis']['genome']['genome_21_fasta_gz'], checkIfExists: true) + ] + """ + } + } + } + + when { + workflow { + """ + GUNZIP.out.gunzip.map { meta, fasta -> + def fa = new File('test.fa') + fa.write(fasta.text.replaceAll('>chr1', '>chr_xxxxxxxxxxxxxxx_1')) + + [ meta, fa.toPath() ] + } + | set { ch_fa } + + def monoploid_seqs = new File('test.mono.seq.txt') + monoploid_seqs.write("chr_xxxxxxxxxxxxxxx_1") + + input[0] = ch_fa + input[1] = Channel.of( [ [ id:'test' ], monoploid_seqs.toPath() ] ) + input[2] = true + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert file(workflow.out.annotation_gff[0][1]).text.contains('Copia_LTR_retrotransposon') }, + { assert file(workflow.out.ltrlib[0][1]).text.contains('#LTR/Copia') }, + { assert workflow.out.lai_log == [] }, + { assert workflow.out.lai_out == [] } + ) + } + } } From 66de1245b3fa523db02a20a5f8f57bd4204e2d2c Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 29 Feb 2024 12:00:17 +1300 Subject: [PATCH 46/65] Add tag uniqueness checks --- assets/schema_xref_assemblies.json | 2 +- lib/WorkflowAssemblyqc.groovy | 34 ++++++++++++++++++++++++++++++ nextflow.config | 5 +++-- tests/stub/stub.config | 2 +- workflows/assemblyqc.nf | 8 +++++++ 5 files changed, 47 insertions(+), 4 deletions(-) diff --git a/assets/schema_xref_assemblies.json b/assets/schema_xref_assemblies.json index e4a5a40a..af118cf3 100644 --- a/assets/schema_xref_assemblies.json +++ b/assets/schema_xref_assemblies.json @@ -1,6 +1,6 @@ { "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/master/assets/schema_input.json", + "$id": "https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/master/assets/schema_xref_assemblies.json", "title": "plant-food-research-open/assemblyqc pipeline - params.synteny_xref_assemblies schema", "description": "Schema for the file provided with params.synteny_xref_assemblies", "type": "array", diff --git a/lib/WorkflowAssemblyqc.groovy b/lib/WorkflowAssemblyqc.groovy index c410665b..5ec2cc32 100755 --- a/lib/WorkflowAssemblyqc.groovy +++ b/lib/WorkflowAssemblyqc.groovy @@ -48,6 +48,40 @@ class WorkflowAssemblyqc { } } + public static ArrayList validateInput(input) { + def inputFields = 5 + def assemblyTags = input[(0..input.size()-1).step(inputFields)] + + def tagCounts = [:] + assemblyTags.each { tag -> + tagCounts[tag] = tagCounts.containsKey(tag) ? tagCounts[tag] + 1 : 1 + } + def repeatedTags = tagCounts.findAll { key, count -> count > 1 }.collect { key, count -> key } + + if (repeatedTags.size() > 0) { + Nextflow.error("Please check input assemblysheet -> Multiple assemblies have the same tags!: ${repeatedTags}") + } + + return input + } + + public static ArrayList validateXrefAssemblies(xref) { + def xrefFields = 3 + def xrefTags = xref[(0..xref.size()-1).step(xrefFields)] + + def tagCounts = [:] + xrefTags.each { tag -> + tagCounts[tag] = tagCounts.containsKey(tag) ? tagCounts[tag] + 1 : 1 + } + def repeatedTags = tagCounts.findAll { key, count -> count > 1 }.collect { key, count -> key } + + if (repeatedTags.size() > 0) { + Nextflow.error("Please check synteny_xref_assemblies -> Multiple xref assemblies have the same tags!: ${repeatedTags}") + } + + return xref + } + public static String jsonifyParams(params) { return JsonOutput.toJson(params).toString() } diff --git a/nextflow.config b/nextflow.config index 795a3f41..662bab59 100644 --- a/nextflow.config +++ b/nextflow.config @@ -145,10 +145,11 @@ profiles { shifter.enabled = false charliecloud.enabled = false apptainer.enabled = false - docker.runOptions = '-u $(id -u):$(id -g)' + // docker.runOptions = '-u $(id -u):$(id -g)' } arm { - docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' + // docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' + docker.runOptions = '--platform=linux/amd64' } singularity { singularity.enabled = true diff --git a/tests/stub/stub.config b/tests/stub/stub.config index cf626085..aafdf6e2 100644 --- a/tests/stub/stub.config +++ b/tests/stub/stub.config @@ -25,7 +25,7 @@ params { hic = 'tests/stub/hic/Dummy_hic.R{1,2}.fq.gz' - synteny_skip = false + synteny_skip = true // GitHub action runner runs out of memory synteny_xref_assemblies = 'https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/xrefsheet.csv' // Limit resources so that this can run on GitHub Actions diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index db45eb06..7f2ca16a 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -65,12 +65,17 @@ include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/du // Info required for completion email and summary def assemblyqc_report = [] +def input_assembly_sheet_fields = 5 +def synteny_xref_assemblies_fields = 3 workflow ASSEMBLYQC { // Input channels ch_versions = Channel.empty() ch_input = Channel.fromSamplesheet('input') + | collect + | flatMap { WorkflowAssemblyqc.validateInput(it) } + | buffer(size: input_assembly_sheet_fields) ch_target_assemby_branch = ch_input | map { tag, fasta, gff, mono_ids, labels -> @@ -126,6 +131,9 @@ workflow ASSEMBLYQC { ch_xref_assembly = params.synteny_skip || ! params.synteny_xref_assemblies ? Channel.empty() : Channel.fromSamplesheet('synteny_xref_assemblies') + | collect + | flatMap { WorkflowAssemblyqc.validateXrefAssemblies(it) } + | buffer(size: synteny_xref_assemblies_fields) | map { tag, fa, labels -> [ tag, file(fa, checkIfExists: true), file(labels, checkIfExists: true) ] } From c1426f1845f2242955643898ddf343a3e6308f4f Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 29 Feb 2024 13:54:39 +1300 Subject: [PATCH 47/65] Removed assemblyqc_report from main workflow --- workflows/assemblyqc.nf | 4 +--- 1 file changed, 1 insertion(+), 3 deletions(-) diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index 7f2ca16a..aefaa6dc 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -63,8 +63,6 @@ include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/du ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// Info required for completion email and summary -def assemblyqc_report = [] def input_assembly_sheet_fields = 5 def synteny_xref_assemblies_fields = 3 @@ -460,7 +458,7 @@ workflow ASSEMBLYQC { workflow.onComplete { if (params.email || params.email_on_fail) { - NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, assemblyqc_report) + NfcoreTemplate.email(workflow, params, summary_params, projectDir, log) } NfcoreTemplate.dump_parameters(workflow, params) NfcoreTemplate.summary(workflow, params, log) From 85c1278cdb9ff2a967108d3cfc92fef2f4f12196 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 29 Feb 2024 14:31:44 +1300 Subject: [PATCH 48/65] Updated the usage doc --- .github/CONTRIBUTING.md | 3 +- .github/PULL_REQUEST_TEMPLATE.md | 2 +- .pre-commit-config.yaml | 10 +++ CHANGELOG.md | 2 +- CITATION.cff | 2 +- README.md | 15 +++- docs/usage.md | 122 +++++++++++++++++++------------ version_check.sh | 14 ++++ 8 files changed, 115 insertions(+), 55 deletions(-) create mode 100755 version_check.sh diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index ab01b3d5..edd71437 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -69,8 +69,7 @@ If you wish to contribute a new step, please use the following coding standards: 6. Add sanity checks and validation for all relevant parameters. 7. Perform local tests to validate that the new code works as expected. 8. If applicable, add a new test command in `.github/workflow/ci.yml`. -9. Update MultiQC config `assets/multiqc_config.yml` so relevant suffixes, file name clean up and module plots are in the appropriate order. If applicable, add a [MultiQC](https://https://multiqc.info/) module. -10. Add a description of the output files and if relevant any appropriate images from the MultiQC report to `docs/output.md`. +9. Add a description of the output files and if relevant any appropriate images from the MultiQC report to `docs/output.md`. ### Default values diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 3da295ca..50053a1f 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -16,7 +16,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/plant-food-r - [ ] This comment contains a description of changes (with reason). - [ ] If you've fixed a bug or added code that should be tested, add tests! - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/plant-food-research-open/assemblyqc/tree/master/.github/CONTRIBUTING.md) -- [ ] Make sure your code lints (`nf-core lint`). +- [ ] Make sure your code lints (`nf-core lint`) and (`pre-commit run --all`) - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index af57081f..bc85d767 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -8,3 +8,13 @@ repos: hooks: - id: editorconfig-checker alias: ec + - repo: local + hooks: + - id: version_checks + name: Version checks + language: system + entry: > + ./version_check.sh + always_run: true + fail_fast: true + pass_filenames: false diff --git a/CHANGELOG.md b/CHANGELOG.md index bad08745..6680321b 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v1.4dev - [28-Feb-2024] +## 1.4dev - [28-Feb-2024] ### `Added` diff --git a/CITATION.cff b/CITATION.cff index efb1b209..bc1311fa 100644 --- a/CITATION.cff +++ b/CITATION.cff @@ -21,7 +21,7 @@ authors: - family-names: "Deng" given-names: "Cecilia" title: "AssemblyQC: A NextFlow pipeline for evaluating assembly quality" -version: 1.4 +version: 1.4dev date-released: 2024-02-12 url: "https://github.com/Plant-Food-Research-Open/assembly_qc" doi: 10.5281/zenodo.10647870 diff --git a/README.md b/README.md index a8c9da60..d2957613 100644 --- a/README.md +++ b/README.md @@ -9,7 +9,7 @@ ## Introduction -**plant-food-research-open/assemblyqc** is a [NextFlow](https://www.nextflow.io/docs/latest/index.html) pipeline which evaluates assembly quality with well-established tools and presents the results in a unified html report. The tools are shown in the [Pipeline Flowchart](#pipeline-flowchart) and their version are listed in [CITATIONS.md](./CITATIONS.md). +**plant-food-research-open/assemblyqc** is a [NextFlow](https://www.nextflow.io/docs/latest/index.html) pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report. The tools are shown in the [Pipeline Flowchart](#pipeline-flowchart) and their version are listed in [CITATIONS.md](./CITATIONS.md). ## Pipeline Flowchart @@ -57,13 +57,14 @@ flowchart LR - [BUSCO](https://gitlab.com/ezlab/busco/-/tree/master): Gene-space completeness estimation - [TIDK](https://github.com/tolkit/telomeric-identifier): Telomere repeat identification - [LAI](https://github.com/oushujun/LTR_retriever/blob/master/LAI): Continuity of repetitive sequences -- [LAI::LTRRETRIEVER](https://github.com/oushujun/LTR_retriever): Repeat identification - [KRAKEN2](https://github.com/DerrickWood/kraken2): Taxonomy classification - [HIC CONTACT MAP](https://github.com/igvteam/juicebox-web): Alignment and visualisation of HiC data - SYNTENY: Synteny analysis using [MUMMER](https://github.com/mummer4/mummer) and [CIRCOS](http://circos.ca/documentation/) ## Usage +Refer to the [usage document](./docs/usage.md) for details. + > [!NOTE] > If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. @@ -88,9 +89,17 @@ nextflow run plant-food-research-open/assemblyqc \ > Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; > see [docs](https://nf-co.re/usage/configuration#custom-configuration-files). +### Quick Start for Plant&Food Users + +Download the pipeline to your `/workspace/$USER` folder. Change the parameters defined in the [pfr/params.json](./pfr/params.json) file. Submit the pipeline to SLURM for execution. + +```bash +sbatch ./pfr_assemblyqc +``` + ## Credits -plant-food-research-open/assemblyqc was originally written by Usman Rashid and Ken Smith. +plant-food-research-open/assemblyqc was originally written by Usman Rashid and Ken Smith. Ross Crowhurst, Chen Wu and Marcus Davy generously contributed their QC scripts. We thank the following people for their extensive assistance in the development of this pipeline: diff --git a/docs/usage.md b/docs/usage.md index 1a98b70a..22c30ae2 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -1,61 +1,94 @@ # plant-food-research-open/assemblyqc: Usage -> _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._ +## Assemblysheet input -## Introduction +You will need to create an assemblysheet with information about the assemblies you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 5 columns, and a header row. An [example assemblysheet](../assets/assemblysheet.csv) has been provided with the pipeline. Its fields are: - +- `tag:` A unique tag which represents the target assembly throughout the pipeline and in the final report +- `fasta:` FASTA file +- `gff3 [Optional]:` GFF3 annotation file if available +- `monoploid_ids [Optional]:` A txt file listing the IDs used to calculate LAI in monoploid mode if necessary +- `synteny_labels [Optional]:` A two column tsv file listing fasta sequence ids (first column) and labels for the synteny plots (second column) when performing synteny analysis -## Samplesheet input +## External databases -You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row as shown in the examples below. +### NCBI FCS GX database + +If NCBI FCS GX foreign organism contamination check is executed by setting `ncbi_fcs_gx_skip` to `false`, the path to the GX database must be provided with option `ncbi_fcs_gx_db_path`. The user must ensure that the database is correctly downloaded and placed in a location accessible to the pipeline. Setup instructions are available at . The database path must contain following files: ```bash ---input '[path to samplesheet file]' +all.assemblies.tsv +all.blast_div.tsv.gz +all.gxi +all.gxs +all.manifest +all.meta.jsonl +all.README.txt +all.seq_info.tsv.gz +all.taxa.tsv ``` -### Multiple runs of the same sample +### Kraken2 -The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: +Path to Kraken2 database is provided by the `kraken2_db_path` parameter. This can be a URL to a public `.tar.gz` file such as `https://genome-idx.s3.amazonaws.com/kraken/k2_pluspfp_20240112.tar.gz`. The pipeline can download and extract the database. This is not the recommended practice owing to the size of the database. Rather, the database should be downloaded, extracted and stored in a read-only location. The path to that location can be provided by the `kraken2_db_path` parameter such as `/workspace/ComparativeDataSources/kraken2db/k2_pluspfp_20230314`. -```csv title="samplesheet.csv" -sample,fastq_1,fastq_2 -CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz -CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz -CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz,AEG588A1_S1_L004_R2_001.fastq.gz -``` +### BUSCO -### Full samplesheet +BUSCO lineage databases are downloaded and updated by the BUSCO tool itself. A persistent location for the database can be provided by specifying `busco_download_path` parameter. -The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 3 columns to match those defined in the table below. +## Other parameters -A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. +### Assemblathon stats -```csv title="samplesheet.csv" -sample,fastq_1,fastq_2 -CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz -CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz -CONTROL_REP3,AEG588A3_S3_L002_R1_001.fastq.gz,AEG588A3_S3_L002_R2_001.fastq.gz -TREATMENT_REP1,AEG588A4_S4_L003_R1_001.fastq.gz, -TREATMENT_REP2,AEG588A5_S5_L003_R1_001.fastq.gz, -TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz, -TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz, -``` +`assemblathon_stats_n_limit` is the number of 'N's for the unknown gap size. This number is used to split the scaffolds into contigs to compute contig-related stats. NCBI's recommendation for unknown gap size is 100 . + +### NCBI FCS adaptor + +`ncbi_fcs_adaptor_empire` should be set to `euk` for Eukaryotes and `prok` for Prokaryotes. + +### NCBI FCS GX + +`ncbi_fcs_gx_tax_id` is the taxonomy ID for all the assemblies listed in the assemblysheet. A taxonomy ID can be obtained by searching a _Genus species_ at . + +### BUSCO + +- `busco_mode`: `geno` or `genome` for genome assemblies (DNA), `tran` or `transcriptome` for transcriptome assemblies (DNA), and `prot` or `proteins` for annotated gene sets (protein). +- `busco_lineage_datasets`: A space-separated list of BUSCO lineages. Any number of lineages can be specified such as "fungi_odb10 hypocreales_odb10". Each assembly is assessed against each of the listed lineage. To select a lineage, refer to . + +### TIDK + +- `tidk_repeat_seq`: The telomere search sequence. To select an appropriate sequence, see . Commonly used sequences are TTTAGGG (Plant), TTAGGG (Fungus, Vertebrates) and TTAGG (Insect). Further reading: +- `tidk_filter_by_size`: Set this flag to `true` to filter out assembly sequences smaller than the size specified by the next parameter (default: `false`). +- `tidk_filter_size_bp`: Minimum size of the assembly sequence processed by TIDK (default: 1000000 (1Mbp)). + +### HiC + +Following parameter must be configured: + +- `hic`: Path to reads provided as a SRA ID or as a path to paired reads with pattern '\*R{1,2}.(fastq|fq).gz' +- `hic_skip_fastp`: Skips fastp trimming +- `hic_skip_fastqc`: Skips QC by fastqc +- `hic_fastp_ext_args`: Additional arguments for fastp (default: '--qualified_quality_phred 20 --length_required 50') -| Column | Description | -| --------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -| `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). | -| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | -| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +### Synteny analysis -An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. +- `synteny_between_input_assemblies`: Set it to `true` to create synteny plots between each pair of input assemblies. Default is `true`. +- `synteny_many_to_many_align`: Set it to `true` to include alignment blocks with many-to-many mappings or set to `false` to only include 1-to-1 mappings. Default is `false`. See the documentation of `dnadiff` for further details: +- `synteny_max_gap`: Alignments within this distance are bundled together. Default: 1000000 (1 Mbp). +- `synteny_min_bundle_size`: After bundling, any bundle smaller than this size is filtered out. Default: 1000 (1 Kbp) +- `synteny_plot_1_vs_all`: Set it to `true` to create a separate synteny plot for each contig of the target assembly versus all contigs of the reference assembly. Set it to `false` to create a single plot for each target assembly against each reference assembly. This joint plot is also created when `plot_1_vs_all` is set to `true`. Default: `false` +- `synteny_color_by_contig`: Set it to `true` to color the synteny plot by contig. Set it to `false` to color the synteny plot by the number of links in a bundle. Default: `true` +- `synteny_xref_assemblies`: Similar to `--input`, this parameter also provides a CSV sheet listing external reference assemblies which are included in the synteny analysis but are not analysed by other QC tools. See the [example xrefsheet](../assets/xrefsheet.csv) included with the pipeline. Its fields are: + - `tag:` A unique tag which represents the reference assembly in the final report + - `fasta:` FASTA file + - `synteny_labels:` A two column tsv file listing fasta sequence ids (first column) and labels for the synteny plots (second column) ## Running the pipeline The typical command for running the pipeline is as follows: ```bash -nextflow run plant-food-research-open/assemblyqc --input ./samplesheet.csv --outdir ./results --genome GRCh37 -profile docker +nextflow run plant-food-research-open/assemblyqc --input ./assemblysheet.csv --outdir ./results -profile docker ``` This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. @@ -73,9 +106,8 @@ If you wish to repeatedly use the same parameters for multiple runs, rather than Pipeline settings can be provided in a `yaml` or `json` file via `-params-file `. -:::warning -Do not use `-c ` to specify parameters as this will result in errors. Custom config files specified with `-c` must only be used for [tuning process resource specifications](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources), other infrastructural tweaks (such as output directories), or module arguments (args). -::: +> [!WARNING] +> Do not use `-c ` to specify parameters as this will result in errors. Custom config files specified with `-c` must only be used for [tuning process resource specifications](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources), other infrastructural tweaks (such as output directories), or module arguments (args). The above pipeline run specified with a params file in yaml format: @@ -88,7 +120,6 @@ with `params.yaml` containing: ```yaml input: './samplesheet.csv' outdir: './results/' -genome: 'GRCh37' <...> ``` @@ -112,15 +143,13 @@ This version number will be logged in reports when you run the pipeline, so that To further assist in reproducbility, you can use share and re-use [parameter files](#running-the-pipeline) to repeat pipeline runs with the same settings without having to write out a command with every single parameter. -:::tip -If you wish to share such profile (such as upload as supplementary material for academic publications), make sure to NOT include cluster specific paths to files, nor institutional specific profiles. -::: +> [!TIP] +> If you wish to share such profile (such as upload as supplementary material for academic publications), make sure to NOT include cluster specific paths to files, nor institutional specific profiles. ## Core Nextflow arguments -:::note -These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen). -::: +> [!NOTE] +> These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen). ### `-profile` @@ -128,9 +157,8 @@ Use this parameter to choose a configuration profile. Profiles can give configur Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Apptainer, Conda) - see below. -:::info -We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. -::: +> [!TIP] +> We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to see if your system is available in these configs please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation). diff --git a/version_check.sh b/version_check.sh new file mode 100755 index 00000000..04a714f7 --- /dev/null +++ b/version_check.sh @@ -0,0 +1,14 @@ +#!/usr/bin/env bash + +config_version=$(sed -n "/^\s*version\s*=\s*'/s/version//p" nextflow.config | tr -d "=[:space:]'") +cff_version=$(sed -n '/^version: /s/version: //p' CITATION.cff | tr -d '[:space:]') + +if [[ $config_version != $cff_version ]]; then + echo 'config_version != cff_version' + exit 1 +fi + +# Check CHANGELOG version + +grep "## $config_version" CHANGELOG.md >/dev/null \ + || (echo 'Failed to match CHANGELOG version'; exit 1) From 071be83d80c4b56d25233539c92e4d71c44386f8 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 29 Feb 2024 15:22:49 +1300 Subject: [PATCH 49/65] Added pfr/params.json --- .gitignore | 4 +++ assets/schema_input.json | 8 +++--- assets/schema_xref_assemblies.json | 4 +-- pfr/params.json | 29 +++++++++++++++++++++ pfr/profile.config | 5 ++++ pfr_assemblyqc | 41 +++++++++++++++++++++++++----- 6 files changed, 79 insertions(+), 12 deletions(-) diff --git a/.gitignore b/.gitignore index 5124c9ac..00c62de8 100644 --- a/.gitignore +++ b/.gitignore @@ -6,3 +6,7 @@ results/ testing/ testing* *.pyc + +# PFR files +*.stdout +*.stderr diff --git a/assets/schema_input.json b/assets/schema_input.json index 981c7786..2aab4188 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -14,13 +14,13 @@ }, "fasta": { "type": "string", - "pattern": "^\\S+\\.f(ast|as|sa|na)?\\.gz$", - "errorMessage": "FASTA file path cannot contain spaces and must have extension '.f(ast|as|sa|na)' or '.f(ast|as|sa|na).gz'" + "pattern": "^\\S+\\.f(a|asta|as|sa|na)(\\.gz)?$", + "errorMessage": "FASTA file path cannot contain spaces and must have extension '.f(a|asta|as|sa|na)' or '.f(a|asta|as|sa|na).gz'" }, "gff3": { "type": "string", - "pattern": "^\\S+\\.gff(3)?\\.gz$", - "errorMessage": "GFF3 file path cannot contain spaces and must have extension '.gff.gz' or '.gff3.gz'" + "pattern": "^\\S+\\.gff(3)?(\\.gz)?$", + "errorMessage": "GFF3 file path cannot contain spaces and must have extension '.gff.gz', '.gff3.gz', '.gff' or '.gff3'" }, "monoploid_ids": { "errorMessage": "Monoploid IDs txt file path cannot contain spaces and must have extension '.txt'", diff --git a/assets/schema_xref_assemblies.json b/assets/schema_xref_assemblies.json index e4a5a40a..084838d0 100644 --- a/assets/schema_xref_assemblies.json +++ b/assets/schema_xref_assemblies.json @@ -14,8 +14,8 @@ }, "fasta": { "type": "string", - "pattern": "^\\S+\\.f(ast|as|sa|na)?\\.gz$", - "errorMessage": "FASTA file path cannot contain spaces and must have extension '.f(ast|as|sa|na)' or '.f(ast|as|sa|na).gz'" + "pattern": "^\\S+\\.f(a|asta|as|sa|na)(\\.gz)?$", + "errorMessage": "FASTA file path cannot contain spaces and must have extension '.f(a|asta|as|sa|na)' or '.f(a|asta|as|sa|na).gz'" }, "synteny_labels": { "errorMessage": "Synteny labels tsv path cannot contain spaces and must have extension '.tsv'", diff --git a/pfr/params.json b/pfr/params.json index e69de29b..1ab49e81 100644 --- a/pfr/params.json +++ b/pfr/params.json @@ -0,0 +1,29 @@ +{ + "input":"/workspace/assemblyqc/testdata/default/assemblysheet.csv", + "ncbi_fcs_adaptor_skip": false, + "ncbi_fcs_adaptor_empire": "euk", + "ncbi_fcs_gx_skip": false, + "ncbi_fcs_gx_tax_id": 3750, + "ncbi_fcs_gx_db_path": "/workspace/ComparativeDataSources/NCBI/FCS/GX/r2023-01-24", + "busco_skip": false, + "busco_mode": "geno", + "busco_lineage_datasets": "embryophyta_odb10 eudicots_odb10", + "busco_download_path": "/workspace/ComparativeDataSources/BUSCO/assemblyqc", + "tidk_skip": false, + "tidk_repeat_seq": "TTTAGGG", + "tidk_filter_by_size": true, + "tidk_filter_size_bp": 1000000, + "lai_skip": false, + "kraken2_skip": false, + "kraken2_db_path": "/workspace/ComparativeDataSources/kraken2db/k2_pluspfp_20230314", + "hic": null, + "synteny_skip": false, + "synteny_between_input_assemblies": true, + "synteny_many_to_many_align": false, + "synteny_max_gap": 1000000, + "synteny_min_bundle_size": 1000, + "synteny_plot_1_vs_all": true, + "synteny_color_by_contig": true, + "synteny_xref_assemblies": "/workspace/assemblyqc/testdata/default/xrefsheet.csv", + "outdir": "./results" +} diff --git a/pfr/profile.config b/pfr/profile.config index f219d135..3a3c18c3 100644 --- a/pfr/profile.config +++ b/pfr/profile.config @@ -10,3 +10,8 @@ profiles { } } } + +params { + config_profile_name = 'Plant&Food profile' + config_profile_description = 'Plant&Food profile using SLURM in combination with Apptainer' +} diff --git a/pfr_assemblyqc b/pfr_assemblyqc index 4f23a416..09df6e22 100644 --- a/pfr_assemblyqc +++ b/pfr_assemblyqc @@ -9,6 +9,22 @@ #SBATCH --error pfr_assemblyqc.stderr #SBATCH --mem=4G +full_test_flag=0 + +# Parse command line options +while getopts "t" opt; do + case ${opt} in + t ) + full_test_flag=1 + ;; + \? ) + echo "Invalid option: $OPTARG" 1>&2 + exit 1 + ;; + esac +done +shift $((OPTIND -1)) + ml unload perl ml apptainer/1.1 ml nextflow/23.04.4 @@ -16,9 +32,22 @@ ml nextflow/23.04.4 export TMPDIR="/workspace/$USER/tmp" export APPTAINER_BINDPATH="$APPTAINER_BINDPATH,$TMPDIR:$TMPDIR,$TMPDIR:/tmp" -nextflow \ - main.nf \ - -c pfr/profile.config \ - -profile pfr,apptainer \ - -resume \ - --params-file pfr/params.json +if [ $full_test_flag -eq 1 ]; then + nextflow \ + main.nf \ + -c pfr/profile.config \ + -profile pfr,apptainer,test_full \ + --ncbi_fcs_gx_skip false \ + --ncbi_fcs_gx_tax_id 35717 \ + --ncbi_fcs_gx_db_path "/workspace/ComparativeDataSources/NCBI/FCS/GX/r2023-01-24" \ + --kraken2_skip false \ + --kraken2_db_path "/workspace/ComparativeDataSources/kraken2db/k2_pluspfp_20230314" \ + -resume +else + nextflow \ + main.nf \ + -c pfr/profile.config \ + -profile pfr,apptainer \ + -params-file pfr/params.json \ + -resume +fi From bfdce22ba5be073957f53a90ea7a36f1aa8cedeb Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 29 Feb 2024 15:40:05 +1300 Subject: [PATCH 50/65] Fixed linting --- pfr/params.json | 2 +- pfr_assemblyqc | 14 +++++++------- 2 files changed, 8 insertions(+), 8 deletions(-) diff --git a/pfr/params.json b/pfr/params.json index 1ab49e81..ab1ea360 100644 --- a/pfr/params.json +++ b/pfr/params.json @@ -1,5 +1,5 @@ { - "input":"/workspace/assemblyqc/testdata/default/assemblysheet.csv", + "input": "/workspace/assemblyqc/testdata/default/assemblysheet.csv", "ncbi_fcs_adaptor_skip": false, "ncbi_fcs_adaptor_empire": "euk", "ncbi_fcs_gx_skip": false, diff --git a/pfr_assemblyqc b/pfr_assemblyqc index 09df6e22..f92ae0d2 100644 --- a/pfr_assemblyqc +++ b/pfr_assemblyqc @@ -13,15 +13,15 @@ full_test_flag=0 # Parse command line options while getopts "t" opt; do - case ${opt} in + case ${opt} in t ) - full_test_flag=1 - ;; + full_test_flag=1 + ;; \? ) - echo "Invalid option: $OPTARG" 1>&2 - exit 1 - ;; - esac + echo "Invalid option: $OPTARG" 1>&2 + exit 1 + ;; + esac done shift $((OPTIND -1)) From 7968784d015b7e04cc22a1101ff2bebfb0fba3a1 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 29 Feb 2024 21:27:55 +1300 Subject: [PATCH 51/65] Now allowing {1,2}.fq.gz pattern for hic --- conf/test_full.config | 4 ++-- docs/usage.md | 2 +- nextflow_schema.json | 4 ++-- 3 files changed, 5 insertions(+), 5 deletions(-) diff --git a/conf/test_full.config b/conf/test_full.config index 4c4b5151..2e8d65ee 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -14,7 +14,7 @@ params { config_profile_name = 'Full test profile' config_profile_description = 'Full test dataset to check pipeline function' - input = 'https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/assemblysheet.csv' + input = 'assets/assemblysheet.csv' ncbi_fcs_adaptor_skip = false ncbi_fcs_adaptor_empire = 'euk' @@ -38,5 +38,5 @@ params { hic = 'SRR8238190' synteny_skip = false - synteny_xref_assemblies = 'https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/xrefsheet.csv' + synteny_xref_assemblies = 'assets/xrefsheet.csv' } diff --git a/docs/usage.md b/docs/usage.md index 22c30ae2..48fdb78e 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -65,7 +65,7 @@ BUSCO lineage databases are downloaded and updated by the BUSCO tool itself. A p Following parameter must be configured: -- `hic`: Path to reads provided as a SRA ID or as a path to paired reads with pattern '\*R{1,2}.(fastq|fq).gz' +- `hic`: Path to reads provided as a SRA ID or as a path to paired reads with pattern '\*{1,2}.(fastq|fq).gz' - `hic_skip_fastp`: Skips fastp trimming - `hic_skip_fastqc`: Skips QC by fastqc - `hic_fastp_ext_args`: Additional arguments for fastp (default: '--qualified_quality_phred 20 --length_required 50') diff --git a/nextflow_schema.json b/nextflow_schema.json index 3299f676..af096137 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -185,8 +185,8 @@ "hic": { "type": "string", "description": "HiC reads", - "help_text": "Path to reads provided as a SRA ID or as a path to paired reads with pattern '*R{1,2}.(fastq|fq).gz'", - "pattern": "^SR\\w+$|^\\S+R\\{1,2\\}\\.f(ast)?q\\.gz$" + "help_text": "Path to reads provided as a SRA ID or as a path to paired reads with pattern '*{1,2}.(fastq|fq).gz'", + "pattern": "^SR\\w+$|^\\S+\\{1,2\\}\\.f(ast)?q\\.gz$" }, "hic_skip_fastp": { "type": "boolean", From d714bf03c9283c033743f856d71f0dd56c2e6ba7 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Fri, 1 Mar 2024 10:45:03 +1300 Subject: [PATCH 52/65] Fixed container paths --- conf/test_full.config | 4 ++-- modules/local/filtersortfasta.nf | 2 +- modules/local/generatekaryotype.nf | 2 +- modules/local/getfastalength.nf | 2 +- modules/local/kraken2.nf | 2 +- modules/local/splitbundlefile.nf | 2 +- tests/stub/stub.config | 4 ++-- 7 files changed, 9 insertions(+), 9 deletions(-) diff --git a/conf/test_full.config b/conf/test_full.config index 2e8d65ee..4c4b5151 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -14,7 +14,7 @@ params { config_profile_name = 'Full test profile' config_profile_description = 'Full test dataset to check pipeline function' - input = 'assets/assemblysheet.csv' + input = 'https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/assemblysheet.csv' ncbi_fcs_adaptor_skip = false ncbi_fcs_adaptor_empire = 'euk' @@ -38,5 +38,5 @@ params { hic = 'SRR8238190' synteny_skip = false - synteny_xref_assemblies = 'assets/xrefsheet.csv' + synteny_xref_assemblies = 'https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/xrefsheet.csv' } diff --git a/modules/local/filtersortfasta.nf b/modules/local/filtersortfasta.nf index 4d291024..4b184abf 100644 --- a/modules/local/filtersortfasta.nf +++ b/modules/local/filtersortfasta.nf @@ -2,7 +2,7 @@ process FILTERSORTFASTA { tag "${target}.on.${reference}" label 'process_single' - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1': 'biocontainers/samtools:1.16.1--h6899075_1' }" diff --git a/modules/local/generatekaryotype.nf b/modules/local/generatekaryotype.nf index 905a0d81..c4ddfb2d 100644 --- a/modules/local/generatekaryotype.nf +++ b/modules/local/generatekaryotype.nf @@ -2,7 +2,7 @@ process GENERATEKARYOTYPE { tag "${target_on_ref}.${seq_tag}" label 'process_single' - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ubuntu:20.04': 'nf-core/ubuntu:20.04' }" diff --git a/modules/local/getfastalength.nf b/modules/local/getfastalength.nf index 3f3748d7..fc12b2ef 100644 --- a/modules/local/getfastalength.nf +++ b/modules/local/getfastalength.nf @@ -2,7 +2,7 @@ process GETFASTALENGTH { tag "${target}.on.${reference}" label 'process_single' - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1': 'biocontainers/samtools:1.16.1--h6899075_1' }" diff --git a/modules/local/kraken2.nf b/modules/local/kraken2.nf index 7fa005f4..53f722ac 100644 --- a/modules/local/kraken2.nf +++ b/modules/local/kraken2.nf @@ -2,7 +2,7 @@ process KRAKEN2 { tag "${asm_tag}" label 'process_single' - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/kraken2:2.1.2--pl5321h9f5acd7_2': 'biocontainers/kraken2:2.1.2--pl5321h9f5acd7_2' }" diff --git a/modules/local/splitbundlefile.nf b/modules/local/splitbundlefile.nf index c9cb4358..3fff1496 100644 --- a/modules/local/splitbundlefile.nf +++ b/modules/local/splitbundlefile.nf @@ -2,7 +2,7 @@ process SPLITBUNDLEFILE { tag "${target_on_ref}" label 'process_single' - container "${ workflow.containerEngine == 'singularity' || workflow.containerEngine == 'apptainer' ? + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ubuntu:20.04': 'nf-core/ubuntu:20.04' }" diff --git a/tests/stub/stub.config b/tests/stub/stub.config index aafdf6e2..4f753187 100644 --- a/tests/stub/stub.config +++ b/tests/stub/stub.config @@ -2,7 +2,7 @@ params { config_profile_name = 'Full stub test' config_profile_description = 'Full test of the pipeline in stub mode' - input = 'https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/assemblysheet.csv' + input = 'assets/assemblysheet.csv' ncbi_fcs_adaptor_skip = false ncbi_fcs_adaptor_empire = 'euk' @@ -26,7 +26,7 @@ params { hic = 'tests/stub/hic/Dummy_hic.R{1,2}.fq.gz' synteny_skip = true // GitHub action runner runs out of memory - synteny_xref_assemblies = 'https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/dev/assets/xrefsheet.csv' + synteny_xref_assemblies = 'assets/xrefsheet.csv' // Limit resources so that this can run on GitHub Actions max_cpus = 2 From f13a9e569cd77f5ca0b004bca24323faa9432d78 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Fri, 1 Mar 2024 10:51:24 +1300 Subject: [PATCH 53/65] Updated fasta_ltrretriever_lai --- modules.json | 2 +- .../pfr/fasta_ltrretriever_lai/main.nf | 16 ++++++---- .../fasta_ltrretriever_lai/tests/main.nf.test | 30 +++++++++++++++++-- 3 files changed, 40 insertions(+), 8 deletions(-) diff --git a/modules.json b/modules.json index 47c20082..a25581b4 100644 --- a/modules.json +++ b/modules.json @@ -81,7 +81,7 @@ "pfr": { "fasta_ltrretriever_lai": { "branch": "main", - "git_sha": "60ee50c79f8f868fe0b2e48cba543ad1d8fa89b2", + "git_sha": "f148f5384395618fc706b6e2f059bd1ce037d06c", "installed_by": ["subworkflows"] }, "fastq_bwa_mem_samblaster": { diff --git a/subworkflows/pfr/fasta_ltrretriever_lai/main.nf b/subworkflows/pfr/fasta_ltrretriever_lai/main.nf index ea5183b1..7bdc2c28 100644 --- a/subworkflows/pfr/fasta_ltrretriever_lai/main.nf +++ b/subworkflows/pfr/fasta_ltrretriever_lai/main.nf @@ -24,7 +24,7 @@ workflow FASTA_LTRRETRIEVER_LAI { ch_short_ids_fasta = ch_fasta | join(CUSTOM_SHORTENFASTAIDS.out.short_ids_fasta, by:0, remainder:true) | map { meta, fasta, short_ids_fasta -> - [ meta, short_ids_fasta ?: fasta ] + if ( fasta ) { [ meta, short_ids_fasta ?: fasta ] } } ch_short_ids_tsv = CUSTOM_SHORTENFASTAIDS.out.short_ids_tsv @@ -35,6 +35,12 @@ workflow FASTA_LTRRETRIEVER_LAI { | map { meta, short_ids_tsv, monoploid_seqs -> map_monoploid_seqs_to_new_ids(meta, short_ids_tsv, monoploid_seqs) } + | collectFile(newLine:true) + | map { seqs -> + def id = seqs.name.split('.mapped.monoploid.seqs.txt')[0] + + [ [ id: id ], seqs ] + } ch_versions = ch_versions.mix(CUSTOM_SHORTENFASTAIDS.out.versions.first()) // MODULE: LTRHARVEST @@ -133,7 +139,7 @@ def map_monoploid_seqs_to_new_ids(meta, short_ids_tsv, monoploid_seqs) { def short_ids_head = short_ids_tsv.text.split('\n')[0] if (short_ids_head == "IDs have acceptable length and character. No change required.") { - return [ meta, monoploid_seqs ] + return [ "${meta.id}.mapped.monoploid.seqs.txt" ] + monoploid_seqs.text.split('\n') } def orig_to_new_ids = [:] @@ -142,15 +148,15 @@ def map_monoploid_seqs_to_new_ids(meta, short_ids_tsv, monoploid_seqs) { orig_to_new_ids[original_id] = renamed_id } - def output_file = new File("${meta.id}.mapped.monoploid.seqs.txt") + def mapped_ids = [] monoploid_seqs.text.eachLine { original_id -> if (!orig_to_new_ids[original_id]) { error "Faild to find $original_id in ${monoploid_seqs}" + "The monoploid_seqs file is malformed!" } - output_file.append(orig_to_new_ids[original_id]) + mapped_ids.add(orig_to_new_ids[original_id]) } - return [ meta, output_file.toPath() ] + return [ "${meta.id}.mapped.monoploid.seqs.txt" ] + mapped_ids } diff --git a/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test b/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test index f2c401d8..7ba88ab6 100644 --- a/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test +++ b/subworkflows/pfr/fasta_ltrretriever_lai/tests/main.nf.test @@ -109,6 +109,8 @@ nextflow_workflow { test("actinidia_chinensis-genome_21_fasta_gz-without_lai") { + options '-stub' + setup { run("GUNZIP") { script "../../../../modules/nf-core/gunzip" @@ -148,11 +150,35 @@ nextflow_workflow { then { assertAll( { assert workflow.success }, - { assert file(workflow.out.annotation_gff[0][1]).text.contains('Copia_LTR_retrotransposon') }, - { assert file(workflow.out.ltrlib[0][1]).text.contains('#LTR/Copia') }, + { assert workflow.out.annotation_gff != null }, + { assert workflow.out.ltrlib != null }, { assert workflow.out.lai_log == [] }, { assert workflow.out.lai_out == [] } ) } } + + test("empty_fasta_stub") { + + options '-stub' + + when { + workflow { + """ + def monoploid_seqs = new File('test.mono.seq.txt') + monoploid_seqs.write("chr_xxxxxxxxxxxxxxx_1") + + input[0] = Channel.empty() + input[1] = Channel.of( [ [ id:'test' ], monoploid_seqs.toPath() ] ) + input[2] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } } From d335747fb6ab900af8785f91771543d325f98168 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Fri, 1 Mar 2024 16:06:32 +1300 Subject: [PATCH 54/65] Updated usage doc --- README.md | 2 +- .../assemblathon_stats.html | 2 +- docs/images/FI1.gt.stat.yml.gene.length.png | Bin 0 -> 271070 bytes docs/images/busco_figure.png | Bin 0 -> 95359 bytes docs/images/mqc_fastqc_adapter.png | Bin 23458 -> 0 bytes docs/images/mqc_fastqc_counts.png | Bin 33918 -> 0 bytes docs/images/mqc_fastqc_quality.png | Bin 55769 -> 0 bytes docs/images/synteny.png | Bin 0 -> 305494 bytes docs/images/tidk.png | Bin 0 -> 62458 bytes docs/output.md | 168 +++++++++++++++--- 10 files changed, 143 insertions(+), 29 deletions(-) create mode 100644 docs/images/FI1.gt.stat.yml.gene.length.png create mode 100644 docs/images/busco_figure.png delete mode 100755 docs/images/mqc_fastqc_adapter.png delete mode 100755 docs/images/mqc_fastqc_counts.png delete mode 100755 docs/images/mqc_fastqc_quality.png create mode 100644 docs/images/synteny.png create mode 100644 docs/images/tidk.png diff --git a/README.md b/README.md index d2957613..64fb92be 100644 --- a/README.md +++ b/README.md @@ -63,7 +63,7 @@ flowchart LR ## Usage -Refer to the [usage document](./docs/usage.md) for details. +Refer to [usage](./docs/usage.md) and [output](./docs/output.md) documents for details. > [!NOTE] > If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. diff --git a/bin/report_modules/templates/assemblathon_stats/assemblathon_stats.html b/bin/report_modules/templates/assemblathon_stats/assemblathon_stats.html index 356b0eaf..56eda984 100644 --- a/bin/report_modules/templates/assemblathon_stats/assemblathon_stats.html +++ b/bin/report_modules/templates/assemblathon_stats/assemblathon_stats.html @@ -12,7 +12,7 @@

    Warning:

    - Contig-related stats are based on the assumption that the n_limit ({{ + Contig-related stats are based on the assumption that the assemblathon_stats_n_limit ({{ all_stats_dicts['PARAMS_DICT']['assemblathon_stats_n_limit'] }}) parameter is specified correctly. If you are not certain of the value of the n_limit parameter, please ignore the contig-related stats.

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z6!c}jeQ83b(j@j4`Dk2^+dha6IEM_0VwK+HzOjAR0J10UAN}JbUlE^O6&ZgQd%Q`QP!K2e`0$yTMd#h3Ve`hrS?p3Tp(?-=oKF9k^*jH^k&3y zL^>QJ@}Eh3#hJ5$j4aGE?Y}|G@XoGLIiN9~Nzr-smpGD?qwriSvnv8$j8_(OVf!m? z9{Wo;L-CqLAM)g!3CBmZx+f&d%AgWwNc_ToR1s%E}tN+jz - ## Pipeline overview The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: -- [FastQC](#fastqc) - Raw read QC -- [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline -- [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution + + +- [FASTA and GFF3 validation](#fasta-and-gff3-validation) +- [Assemblathon stats](#assemblathon-stats) +- [Genometools gt stat](#genometools-gt-stat) +- [NCBI FCS adaptor](#ncbi-fcs-adaptor) +- [NCBI FCS GX](#ncbi-fcs-gx) +- [BUSCO](#busco) +- [TIDK](#tidk) +- [LAI](#lai) +- [Kraken2](#kraken2) +- [HiC contact map](#hic-contact-map) +- [Synteny](#synteny) +- [Pipeline information](#pipeline-information) + +### FASTA and GFF3 validation + +The pipeline prints a warning in the pipeline log if FASTA or GFF3 validation fails. The error log from the validator is reported in the `report.html`. The remaining QC tools are skipped for the assembly with invalid fasta file. -### FastQC +### Assemblathon stats
    Output files -- `fastqc/` - - `*_fastqc.html`: FastQC report containing quality metrics. - - `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images. +- `assemblathon_stats/` + - `*_stats.csv`: Assembly stats in CSV format.
    -[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). +`assemblathon_stats.pl` is a script which calculate a basic set of metrics from a genome assembly. -![MultiQC - FastQC sequence counts plot](images/mqc_fastqc_counts.png) +> [!WARNING] +> Contig-related stats are based on the assumption that `assemblathon_stats_n_limit` is specified correctly. If you are not certain of the value of `assemblathon_stats_n_limit`, please ignore the contig-related stats. -![MultiQC - FastQC mean quality scores plot](images/mqc_fastqc_quality.png) +### Genometools gt stat -![MultiQC - FastQC adapter content plot](images/mqc_fastqc_adapter.png) +
    +Output files -:::note -The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. -::: +- `genometools_gt_stat/` + - `*.gt.stat.yml`: Assembly annotation stats in yaml format. -### MultiQC +
    + +GenomeTools `gt stat` tool calculates a basic set of statistics about features contained in GFF3 files. + +
    AssemblyQC - GenomeTools gt stat gene length distribution
    AssemblyQC - GenomeTools gt stat gene length distribution
    + +### NCBI FCS adaptor
    Output files -- `multiqc/` - - `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser. - - `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline. - - `multiqc_plots/`: directory containing static images from the report in various formats. +- `ncbi_fcs_adaptor/` + - `*_fcs_adaptor_report.tsv`: NCBI FCS adaptor report in CSV format.
    -[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. +[FCS-adaptor detects](https://github.com/ncbi/fcs/wiki/FCS-adaptor#rules-for-action-assignment) adaptor and vector contamination in genome sequences. -Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see . +### NCBI FCS GX -### Pipeline information +### BUSCO
    Output files +- `busco/` + - `busco_figure.png`: Summary figure created from all the BUSCO summaries. + - `tag` + - `short_summary.specific.*_odb10.tag_*.txt`: BUSCO summary for the assembly represented by `tag`. + +
    + +[BUSCO estimates](https://busco.ezlab.org/busco_userguide.html) the completeness and redundancy of processed genomic data based on universal single-copy orthologs. + +
    AssemblyQC - BUSCO summary plot
    AssemblyQC - BUSCO summary plot
    + +### TIDK + +
    +Output files + +- `tidk/` + - `*.apriori.tsv`: Frequencies for successive windows in forward and reverse directions for the pre-specified telomere-repeat sequence. + - `*.apriori.svg`: Plot of `*.apriori.tsv`. + - `*.tidk.explore.tsv`: List of the most frequent repeat sequences. + - `*.top.sequence.txt`: The top sequence from `*.tidk.explore.tsv`. + - `*.aposteriori.tsv`: Frequencies for successive windows in forward and reverse directions for the top sequence from `*.top.sequence.txt`. + - `*.aposteriori.svg`: Plot of `*.aposteriori.tsv`. + +
    + +TIDK toolkit is designed to [identify and visualize](https://github.com/tolkit/telomeric-identifier) telomeric repeats for the Darwin Tree of Life genomes. + +
    AssemblyQC - TIDK plot
    AssemblyQC - TIDK plot
    + +### LAI + +
    +Output files + +- `lai/` + - `*.LAI.log`: Log file from LAI + - `*.LAI.out`: Output file from LAI which lists assembly index by contig and for the whole genome. + - `*.LTRlib.fa`: Long terminal repeat library generated by LTR_retriever. + - `*.restored.ids.gff3`: Long terminal repeat annotation generated by LTR_retriever. + - `*.short.ids.tsv`: LTR_retriever and LAI require that the assembly sequence IDs are alphanumeric and not more than 13 characters long. If needed, the pipeline shortens these IDS. The new and original IDs are listed in this TSV file. + +
    + +LTR Assembly Index (LAI) is a reference-free genome metric that [evaluates assembly continuity](https://doi.org/10.1093/nar/gky730) using LTR-RTs. LTR retrotransposons (LTR-RTs) are the predominant interspersed repeat that is poorly assembled in draft genomes. Correcting for LTR-RT amplification dynamics, LAI is independent of genome size, genomic LTR-RT content, and gene space evaluation metrics such as BUSCO. LAI = Raw LAI + 2.8138 × (94 – whole genome LTR identity). The LAI is set to 0 when raw LAI = 0 or the adjustment produces a negative value. Raw LAI = (Intact LTR element length / Total LTR sequence length) \* 100 + +### Kraken2 + +### HiC contact map + +
    +Output files + +- `hic/` + - `fastqc_raw/` + - `*_1_fastqc.html/*_2_fastqc.html`: FASTQC html report for the raw reads + - `*_1_fastqc.zip/*_2_fastqc.zip`: FASTQC stats for the raw reads + - `fastp/` + - `*.fastp.html`: FASTP HTML report + - `*.fastp.json`: FASTP statistics in JSON format + - `*.fastp.log`: FASTP log + - `*_1.fastp.fastq.gz/*_2.fastp.fastq.gz`: Reads passed by FASTP + - `*_1.fail.fastq.gz/*_2.fail.fastq.gz`: Reads failed by FASTP + - `fastqc_trim/` + - `*_1_fastqc.html/*_2_fastqc.html`: FASTQC html report for the reads passed by FASTP + - `*_1_fastqc.zip/*_2_fastqc.zip`: FASTQC stats for the reads passed by FASTP + - `hicqc` + - `*.on.*_qc_report.pdf`: HiC QC report for reads mapped to an assembly. + - `assembly/` + - `*.agp.assembly`: AGP assembly file listing the length of each contig in the assembly + - `bedpe/` - `*.assembly.bedpe`: `*.agp.assembly` file converted to BEDPE to highlight the contigs on the HiC contact map. +
    + +Hi-C contact mapping experiments measure the frequency of physical contact between loci in the genome. The resulting dataset, called a “contact map,” is represented using a [two-dimensional heatmap](https://github.com/igvteam/juicebox.js) where the intensity of each pixel indicates the frequency of contact between a pair of loci. + +### Synteny + +
    +Output files + +- `synteny/` + - `*.*.all/`: Synteny files corresponding to all contigs of the target assembly with respect to all contig of the reference assembly. + - `*.on.*.all.png/svg`: Synteny plot generated with CIRCOS. + - `bundled.links.tsv`: Bundled links file generated with MUMMER and `dnadiff.pl`. + - `circos.conf`: CIRCOS configuration file used to generate the synteny plot. + - `karyotype.tsv`: Karyotype TSV file used to generate the synteny plot. + - `*.on.*.*`: Synteny files corresponding to of a single contig of the target assembly with respect to all contig of the reference assembly. +
    + +Synteny plots are created with Circos which is a tool [facilitating](https://circos.ca) the identification and analysis of similarities and differences arising from comparisons of genomes. The genome-wide alignments are performed with [MUMMER](https://github.com/mummer4/mummer?tab=readme-ov-file) and bundled with [`dnadiff.pl`](https://github.com/mummer4/mummer/blob/master/scripts/dnadiff.pl). + +
    AssemblyQC - Synteny plot
    AssemblyQC - Synteny plot
    + +### Pipeline information + +
    +Output files + - `pipeline_info/` - - Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`. + - Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.html`. - Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline. - - Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`. - Parameters used by the pipeline run: `params.json`.
    From 218a7abacd67164e9a89e0589d10931b0e5474a9 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Fri, 1 Mar 2024 16:36:14 +1300 Subject: [PATCH 55/65] Fixed multi GX launch bug --- subworkflows/local/ncbi_fcs_gx.nf | 6 ++++-- workflows/assemblyqc.nf | 7 +++---- 2 files changed, 7 insertions(+), 6 deletions(-) diff --git a/subworkflows/local/ncbi_fcs_gx.nf b/subworkflows/local/ncbi_fcs_gx.nf index 0425a916..d3e1bfe7 100644 --- a/subworkflows/local/ncbi_fcs_gx.nf +++ b/subworkflows/local/ncbi_fcs_gx.nf @@ -5,7 +5,7 @@ include { NCBI_FCS_GX_KRONA_PLOT } from '../../modules/local/ncbi_fcs_gx_ workflow NCBI_FCS_GX { take: tuple_of_tag_file - db_path // channel: path + db_path // val: String tax_id // val: Integer main: @@ -20,9 +20,11 @@ workflow NCBI_FCS_GX { ch_versions = ch_versions.mix(NCBI_FCS_GX_SETUP_SAMPLE.out.versions.first()) // MODULE: NCBI_FCS_GX_SCREEN_SAMPLES + ch_db = Channel.of( file(db_path, checkIfExists:true) ) + NCBI_FCS_GX_SCREEN_SAMPLES( ch_all_samples, - db_path, + ch_db, tax_id ) diff --git a/workflows/assemblyqc.nf b/workflows/assemblyqc.nf index aefaa6dc..38217ec5 100644 --- a/workflows/assemblyqc.nf +++ b/workflows/assemblyqc.nf @@ -237,15 +237,14 @@ workflow ASSEMBLYQC { ch_versions = ch_versions.mix(NCBI_FCS_ADAPTOR.out.versions.first()) // SUBWORKFLOW: NCBI_FCS_GX - ch_fcs_gx_inputs = params.ncbi_fcs_gx_skip + ch_fcs_gx_input_assembly = params.ncbi_fcs_gx_skip ? Channel.empty() : ch_valid_target_assembly | map { meta, fa -> [ meta.id, fa ] } - | combine( Channel.of(file(params.ncbi_fcs_gx_db_path, checkIfExists:true)) ) NCBI_FCS_GX( - ch_fcs_gx_inputs.map { tag, fa, db -> [ tag, fa ] }, - ch_fcs_gx_inputs.map { tag, fa, db -> db }, + ch_fcs_gx_input_assembly, + params.ncbi_fcs_gx_db_path ?: [], params.ncbi_fcs_gx_tax_id ?: [] ) From 05f6f4fd646c99529390e27ff802068eb511e2d0 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Fri, 1 Mar 2024 16:43:04 +1300 Subject: [PATCH 56/65] Fixed null db path bug --- subworkflows/local/ncbi_fcs_gx.nf | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/subworkflows/local/ncbi_fcs_gx.nf b/subworkflows/local/ncbi_fcs_gx.nf index d3e1bfe7..cc54292f 100644 --- a/subworkflows/local/ncbi_fcs_gx.nf +++ b/subworkflows/local/ncbi_fcs_gx.nf @@ -20,7 +20,9 @@ workflow NCBI_FCS_GX { ch_versions = ch_versions.mix(NCBI_FCS_GX_SETUP_SAMPLE.out.versions.first()) // MODULE: NCBI_FCS_GX_SCREEN_SAMPLES - ch_db = Channel.of( file(db_path, checkIfExists:true) ) + ch_db = ! db_path + ? Channel.empty() + : Channel.of( file(db_path, checkIfExists:true) ) NCBI_FCS_GX_SCREEN_SAMPLES( ch_all_samples, From cbebbf75104cb4eb933466948c0418736361ff79 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Fri, 1 Mar 2024 17:23:27 +1300 Subject: [PATCH 57/65] Added parameters.md file --- README.md | 2 +- assets/email_template.html | 2 +- docs/README.md | 4 +- docs/parameters.md | 121 +++++++++++++++++++++++++++++++++++++ docs/usage.md | 2 - nextflow.config | 2 +- nextflow_schema.json | 2 +- 7 files changed, 128 insertions(+), 7 deletions(-) create mode 100644 docs/parameters.md diff --git a/README.md b/README.md index 64fb92be..9efd34af 100644 --- a/README.md +++ b/README.md @@ -63,7 +63,7 @@ flowchart LR ## Usage -Refer to [usage](./docs/usage.md) and [output](./docs/output.md) documents for details. +Refer to [usage](./docs/usage.md), [parameters](./docs/parameters.md) and [output](./docs/output.md) documents for details. > [!NOTE] > If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. diff --git a/assets/email_template.html b/assets/email_template.html index be11e5e0..5a56215f 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -4,7 +4,7 @@ - + plant-food-research-open/assemblyqc Pipeline Report diff --git a/docs/README.md b/docs/README.md index 20428bd5..e761e2d5 100644 --- a/docs/README.md +++ b/docs/README.md @@ -3,6 +3,8 @@ The plant-food-research-open/assemblyqc documentation is split into the following pages: - [Usage](usage.md) - - An overview of how the pipeline works, how to run it and a description of all of the different command-line flags. + - An overview of how the pipeline works, how to run it. +- [Parameters](parameters.md) + - A description of all of the different command-line flags. - [Output](output.md) - An overview of the different results produced by the pipeline and how to interpret them. diff --git a/docs/parameters.md b/docs/parameters.md new file mode 100644 index 00000000..5be39404 --- /dev/null +++ b/docs/parameters.md @@ -0,0 +1,121 @@ +# plant-food-research-open/assemblyqc pipeline parameters + +A NextFlow pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report. + +## Input/output options + +| Parameter | Description | Type | Default | Required | Hidden | +| --------- | ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -------- | --------- | -------- | ------ | +| `input` | Input assembly sheet in CSV format
    HelpFASTA and other associated files for input assemblies provided as a formatted CSV file
    | +| `outdir` | The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure. | `string` | ./results | True | | +| `email` | Email address for completion summary.
    HelpSet this parameter to your e-mail address to get a summary e-mail with details of the run sent to you | + +## General stats options + +| Parameter | Description | Type | Default | Required | Hidden | +| ---------------------------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------- | ---- | ------- | -------- | ------ | +| `assemblathon_stats_n_limit` | The number of 'N's for the unknown gap size
    HelpThis number is used to split the scaffolds into contigs to compute contig-r | + +## NCBI FCS options + +| Parameter | Description | Type | Default | Required | Hidden | +| ------------------------- | --------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ | +| `ncbi_fcs_adaptor_skip` | Skip NCBI FCS Adaptor checking | `boolean` | True | | | +| `ncbi_fcs_adaptor_empire` | Empire for NCBI FCS Adaptor checking
    Help'euk' for eukaryotes, or 'prok' for prokaryotes
    | `string` | | | +| `ncbi_fcs_gx_skip` | Skip NCBI FCS external organism contamination checking | `boolean` | True | | | +| `ncbi_fcs_gx_tax_id` | Tax ID for NCBI FCS GX
    HelpGet correct tax ID from https://www.ncbi.nlm.nih.gov/taxonomy
    | `number` | | | | +| `ncbi_fcs_gx_db_path` | Path to NCBI FCS GX database
    HelpNCBI FCS GX DB path

    Due to enormity of the DB size, the pipeline does NOT download the dat | + +## BUSCO options + +| Parameter | Description | Type | Default | Required | Hidden | +| ------------------------ | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ | --------- | ------- | -------- | ------ | +| `busco_skip` | Skip BUSCO | `boolean` | True | | | +| `busco_mode` | BUSCO mode
    Help'geno' or 'genome' for genome assemblies (DNA), 'tran' or 'transcriptome' for transcriptome assemblies (DNA), 'prot' or 'pro | +| `busco_lineage_datasets` | BUSCO lineages
    HelpEach input assembly is assessed against each lineage. It should be provided as a space-separated list of lin | +| `busco_download_path` | Download path for BUSCO
    HelpBUSCO DB download path

    The pipeline automatically downloads the required DB if needed    HelpPlant: TTTAGGG, Fungus, Vertebrates: TTAGGG, Insect: TTAGG
    | `string` | | | | +| `tidk_filter_by_size` | Filter size in base-pairs | `boolean` | | | | +| `tidk_filter_size_bp` | Filter size in base-pairs | `integer` | 1000000 | | | + +## LAI options + +| Parameter | Description | Type | Default | Required | Hidden | +| ---------- | ------------------- | --------- | ------- | -------- | ------ | +| `lai_skip` | Skip LAI estimation | `boolean` | True | | | + +## Kraken2 options + +| Parameter | Description | Type | Default | Required | Hidden | +| ----------------- | --------------------- | --------- | ------- | -------- | ------ | +| `kraken2_skip` | Skip Kraken2 | `boolean` | True | | | +| `kraken2_db_path` | Kraken2 database path | `string` | | | | + +## HiC options + +| Parameter | Description | Type | Default | Required | Hidden | +| -------------------- | --------------------------------------------------------------------------------------------------------------------------------------------- | ------------------------- | ------------------------------------------------- | -------- | ------ | +| `hic` | HiC reads
    HelpPath to reads provided as a SRA ID or as a path to paired reads with pattern '\*{1,2}.(fastq | fq).gz'
    | `string` | | +| `hic_skip_fastp` | Skip HiC read trimming | `boolean` | | | | +| `hic_skip_fastqc` | Skip HiC read QC | `boolean` | | | | +| `hic_fastp_ext_args` | Additional parameters for fastp trimming | `string` | --qualified_quality_phred 20 --length_required 50 | | | + +## Synteny options + +| Parameter | Description | Type | Default | Required | Hidden | +| ---------------------------------- | ----------------------------------------------------------------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ | +| `synteny_skip` | Skip synteny analysis | `boolean` | True | | | +| `synteny_between_input_assemblies` | Create syntenic plots between each pair of input assemblies | `boolean` | True | | | +| `synteny_many_to_many_align` | Include alignment blocks with many-to-many mappings (dnadiff .mcoords file) | `boolean` | | | | +| `synteny_max_gap` | Alignments within this distance are bundled together | `integer` | 1000000 | | | +| `synteny_min_bundle_size` | After bundling, any bundle smaller than this size is filtered out | `integer` | 1000 | | | +| `synteny_plot_1_vs_all` | Create a separate synteny plot for each contig of the target assembly versus all contigs of the reference assembly | `boolean` | True | | | +| `synteny_color_by_contig` | Synteny plot is colored by contig | `boolean` | True | | | +| `synteny_xref_assemblies` | Reference assemblies for synteny analysis
    HelpFASTA and synteny label tsv files should be provided in a formatted CSV file HelpUse to set an upper-limit for the CPU requirement for each process. Shou | +| `max_memory` | Maximum amount of memory that can be requested for any single job.
    HelpUse to set an upper-limit for the memory requirement for each proces | +| `max_time` | Maximum amount of time that can be requested for any single job.
    HelpUse to set an upper-limit for the time requirement for each process. Sho | + +## Institutional config options + +Parameters used to describe centralised config profiles. These should not be edited. + +| Parameter | Description | Type | Default | Required | Hidden | +| ---------------------------- | ---------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -------- | ------- | -------- | ------ | +| `custom_config_version` | Git commit id for Institutional configs. | `string` | master | | True | +| `custom_config_base` | Base directory for Institutional configs.
    HelpIf you're running offline, Nextflow will not be able to fetch the institutional confi | +| `config_profile_name` | Institutional config name. | `string` | | | True | +| `config_profile_description` | Institutional config description. | `string` | | | True | +| `config_profile_contact` | Institutional config contact information. | `string` | | | True | +| `config_profile_url` | Institutional config URL link. | `string` | | | True | + +## Generic options + +Less common options for the pipeline, typically set in a config file. + +| Parameter | Description | Type | Default | Required | Hidden | +| ---------------------------------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ | +| `help` | Display help text. | `boolean` | | | True | +| `version` | Display version and exit. | `boolean` | | | True | +| `publish_dir_mode` | Method used to save pipeline results to output directory.
    HelpThe Nextflow `publishDir` option specifies which intermediate files sho | +| `email_on_fail` | Email address for completion summary, only when pipeline fails.
    HelpAn email address to send a summary email to when the pipeline is com | +| `plaintext_email` | Send plain-text email instead of HTML. | `boolean` | | | True | +| `monochrome_logs` | Do not use coloured log outputs. | `boolean` | | | True | +| `hook_url` | Incoming hook URL for messaging service
    HelpIncoming hook URL for messaging service. Currently, MS Teams and Slack are supported.HelpBy default, parameters set as _hidden_ in the schema are not shown on the command l | +| `validationFailUnrecognisedParams` | Validation of parameters fails when an unrecognised parameter is found.
    HelpBy default, when an unrecognised paramete | +| `validationLenientMode` | Validation of parameters in lenient more.
    HelpAllows string values that are parseable as numbers or booleans. For further inform | diff --git a/docs/usage.md b/docs/usage.md index 48fdb78e..18a454df 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -63,8 +63,6 @@ BUSCO lineage databases are downloaded and updated by the BUSCO tool itself. A p ### HiC -Following parameter must be configured: - - `hic`: Path to reads provided as a SRA ID or as a path to paired reads with pattern '\*{1,2}.(fastq|fq).gz' - `hic_skip_fastp`: Skips fastp trimming - `hic_skip_fastqc`: Skips QC by fastqc diff --git a/nextflow.config b/nextflow.config index 662bab59..0910481d 100644 --- a/nextflow.config +++ b/nextflow.config @@ -259,7 +259,7 @@ manifest { name = 'plant-food-research-open/assemblyqc' author = """Usman Rashid, Ken Smith, Ross Crowhurst, Chen Wu, Marcus Davy""" homePage = 'https://github.com/plant-food-research-open/assemblyqc' - description = """A NextFlow pipeline which evaluates assembly quality with well-established tools and presents the results in a unified html report.""" + description = """A NextFlow pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report.""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' version = '1.4dev' diff --git a/nextflow_schema.json b/nextflow_schema.json index af096137..f16a18ba 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -2,7 +2,7 @@ "$schema": "http://json-schema.org/draft-07/schema", "$id": "https://raw.githubusercontent.com/plant-food-research-open/assemblyqc/master/nextflow_schema.json", "title": "plant-food-research-open/assemblyqc pipeline parameters", - "description": "A NextFlow pipeline which evaluates assembly quality with well-established tools and presents the results in a unified html report.", + "description": "A NextFlow pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report.", "type": "object", "definitions": { "input_output_options": { From 53c75286d3fd9fd8c9b52a77411aa45201741ff2 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Fri, 1 Mar 2024 17:30:01 +1300 Subject: [PATCH 58/65] Crossed out conda --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index 9efd34af..ba0154c5 100644 --- a/README.md +++ b/README.md @@ -2,7 +2,7 @@ [![GitHub Actions Linting Status](https://github.com/plant-food-research-open/assemblyqc/workflows/nf-core%20linting/badge.svg)](https://github.com/plant-food-research-open/assemblyqc/actions?query=workflow%3A%22nf-core+linting%22)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.10647870-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.10647870) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) -[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) +[![run with conda ❌](http://img.shields.io/badge/run%20with-conda%20❌-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) [![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/plant-food-research-open/assemblyqc) From e4d42899f5eaa69c313d10f7fff6f05d173932fe Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Fri, 1 Mar 2024 17:38:47 +1300 Subject: [PATCH 59/65] Fixed output formatting --- docs/output.md | 35 +++++++++++++++++++---------------- 1 file changed, 19 insertions(+), 16 deletions(-) diff --git a/docs/output.md b/docs/output.md index fc6b240b..2d8fd71c 100644 --- a/docs/output.md +++ b/docs/output.md @@ -12,18 +12,21 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d -- [FASTA and GFF3 validation](#fasta-and-gff3-validation) -- [Assemblathon stats](#assemblathon-stats) -- [Genometools gt stat](#genometools-gt-stat) -- [NCBI FCS adaptor](#ncbi-fcs-adaptor) -- [NCBI FCS GX](#ncbi-fcs-gx) -- [BUSCO](#busco) -- [TIDK](#tidk) -- [LAI](#lai) -- [Kraken2](#kraken2) -- [HiC contact map](#hic-contact-map) -- [Synteny](#synteny) -- [Pipeline information](#pipeline-information) +- [plant-food-research-open/assemblyqc: Output](#plant-food-research-openassemblyqc-output) + - [Introduction](#introduction) + - [Pipeline overview](#pipeline-overview) + - [FASTA and GFF3 validation](#fasta-and-gff3-validation) + - [Assemblathon stats](#assemblathon-stats) + - [Genometools gt stat](#genometools-gt-stat) + - [NCBI FCS adaptor](#ncbi-fcs-adaptor) + - [NCBI FCS GX](#ncbi-fcs-gx) + - [BUSCO](#busco) + - [TIDK](#tidk) + - [LAI](#lai) + - [Kraken2](#kraken2) + - [HiC contact map](#hic-contact-map) + - [Synteny](#synteny) + - [Pipeline information](#pipeline-information) ### FASTA and GFF3 validation @@ -56,7 +59,7 @@ The pipeline prints a warning in the pipeline log if FASTA or GFF3 validation fa GenomeTools `gt stat` tool calculates a basic set of statistics about features contained in GFF3 files. -
    AssemblyQC - GenomeTools gt stat gene length distribution
    AssemblyQC - GenomeTools gt stat gene length distribution
    +
    AssemblyQC - GenomeTools gt stat gene length distribution
    AssemblyQC - GenomeTools gt stat gene length distribution
    ### NCBI FCS adaptor @@ -86,7 +89,7 @@ GenomeTools `gt stat` tool calculates a basic set of statistics about features c [BUSCO estimates](https://busco.ezlab.org/busco_userguide.html) the completeness and redundancy of processed genomic data based on universal single-copy orthologs. -
    AssemblyQC - BUSCO summary plot
    AssemblyQC - BUSCO summary plot
    +
    AssemblyQC - BUSCO summary plot
    AssemblyQC - BUSCO summary plot
    ### TIDK @@ -105,7 +108,7 @@ GenomeTools `gt stat` tool calculates a basic set of statistics about features c TIDK toolkit is designed to [identify and visualize](https://github.com/tolkit/telomeric-identifier) telomeric repeats for the Darwin Tree of Life genomes. -
    AssemblyQC - TIDK plot
    AssemblyQC - TIDK plot
    +
    AssemblyQC - TIDK plot
    AssemblyQC - TIDK plot
    ### LAI @@ -168,7 +171,7 @@ Hi-C contact mapping experiments measure the frequency of physical contact betwe Synteny plots are created with Circos which is a tool [facilitating](https://circos.ca) the identification and analysis of similarities and differences arising from comparisons of genomes. The genome-wide alignments are performed with [MUMMER](https://github.com/mummer4/mummer?tab=readme-ov-file) and bundled with [`dnadiff.pl`](https://github.com/mummer4/mummer/blob/master/scripts/dnadiff.pl). -
    AssemblyQC - Synteny plot
    AssemblyQC - Synteny plot
    +
    AssemblyQC - Synteny plot
    AssemblyQC - Synteny plot
    ### Pipeline information From eb121bda48b91f2402cbe1ade53776c919656a2a Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Fri, 1 Mar 2024 17:45:07 +1300 Subject: [PATCH 60/65] Formatted output figs --- docs/output.md | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/docs/output.md b/docs/output.md index 2d8fd71c..52c62fd8 100644 --- a/docs/output.md +++ b/docs/output.md @@ -59,7 +59,7 @@ The pipeline prints a warning in the pipeline log if FASTA or GFF3 validation fa GenomeTools `gt stat` tool calculates a basic set of statistics about features contained in GFF3 files. -
    AssemblyQC - GenomeTools gt stat gene length distribution
    AssemblyQC - GenomeTools gt stat gene length distribution
    +
    AssemblyQC - GenomeTools gt stat gene length distribution
    AssemblyQC - GenomeTools gt stat gene length distribution
    ### NCBI FCS adaptor @@ -89,7 +89,7 @@ GenomeTools `gt stat` tool calculates a basic set of statistics about features c [BUSCO estimates](https://busco.ezlab.org/busco_userguide.html) the completeness and redundancy of processed genomic data based on universal single-copy orthologs. -
    AssemblyQC - BUSCO summary plot
    AssemblyQC - BUSCO summary plot
    +
    AssemblyQC - BUSCO summary plot
    AssemblyQC - BUSCO summary plot
    ### TIDK @@ -108,7 +108,7 @@ GenomeTools `gt stat` tool calculates a basic set of statistics about features c TIDK toolkit is designed to [identify and visualize](https://github.com/tolkit/telomeric-identifier) telomeric repeats for the Darwin Tree of Life genomes. -
    AssemblyQC - TIDK plot
    AssemblyQC - TIDK plot
    +
    AssemblyQC - TIDK plot
    AssemblyQC - TIDK plot
    ### LAI @@ -171,7 +171,7 @@ Hi-C contact mapping experiments measure the frequency of physical contact betwe Synteny plots are created with Circos which is a tool [facilitating](https://circos.ca) the identification and analysis of similarities and differences arising from comparisons of genomes. The genome-wide alignments are performed with [MUMMER](https://github.com/mummer4/mummer?tab=readme-ov-file) and bundled with [`dnadiff.pl`](https://github.com/mummer4/mummer/blob/master/scripts/dnadiff.pl). -
    AssemblyQC - Synteny plot
    AssemblyQC - Synteny plot
    +
    AssemblyQC - Synteny plot
    AssemblyQC - Synteny plot
    ### Pipeline information From a4c649046e2fdb08d05fb4acc21c9a75281be4d4 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 4 Mar 2024 11:26:12 +1300 Subject: [PATCH 61/65] Updated docs and removed bugs in version capture for awk and grep --- docs/images/hic_map.png | Bin 0 -> 2267063 bytes docs/output.md | 45 +++++++++++++++++++---------- modules/local/generatekaryotype.nf | 4 +-- modules/local/splitbundlefile.nf | 2 +- 4 files changed, 32 insertions(+), 19 deletions(-) create mode 100644 docs/images/hic_map.png diff --git a/docs/images/hic_map.png b/docs/images/hic_map.png new file mode 100644 index 0000000000000000000000000000000000000000..e33868cd2c9d9b294860c68bf1d2feb40f363f35 GIT binary patch literal 2267063 zcmeFZWmFy8);5R+mq2h$2*EwLLxP0hF2OhM?yf--Ja`~zaMz9R;O_43?p>U7?|bg| zp4`ztdi0;}!x)=gRMoP%W=(mXS%k{Th@&ABB11tzp-D=-Q-Fek2R_dn5T5~O;I!|4 zK|!Gknu?0bNs5Y+%h_2QnOcCLpd>=$R1wq^yK&PrzeWq0K*MDREWmvs6#9V3;B)qB z3Q^KWP9GKSId6U5(#(4}i>-nXeT0t4DfJgb7w@0LgFewu#c|SP>^7e?oqF0IdOvcX 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Output](#plant-food-research-openassemblyqc-output) - - [Introduction](#introduction) - - [Pipeline overview](#pipeline-overview) - - [FASTA and GFF3 validation](#fasta-and-gff3-validation) - - [Assemblathon stats](#assemblathon-stats) - - [Genometools gt stat](#genometools-gt-stat) - - [NCBI FCS adaptor](#ncbi-fcs-adaptor) - - [NCBI FCS GX](#ncbi-fcs-gx) - - [BUSCO](#busco) - - [TIDK](#tidk) - - [LAI](#lai) - - [Kraken2](#kraken2) - - [HiC contact map](#hic-contact-map) - - [Synteny](#synteny) - - [Pipeline information](#pipeline-information) +- [FASTA and GFF3 validation](#fasta-and-gff3-validation) +- [Assemblathon stats](#assemblathon-stats) +- [Genometools gt stat](#genometools-gt-stat) +- [NCBI FCS adaptor](#ncbi-fcs-adaptor) +- [NCBI FCS GX](#ncbi-fcs-gx) +- [BUSCO](#busco) +- [TIDK](#tidk) +- [LAI](#lai) +- [Kraken2](#kraken2) +- [HiC contact map](#hic-contact-map) +- [Synteny](#synteny) +- [Pipeline information](#pipeline-information) ### FASTA and GFF3 validation @@ -75,6 +72,20 @@ GenomeTools `gt stat` tool calculates a basic set of statistics about features c ### NCBI FCS GX +
    +Output files + +- `ncbi_fcs_gx/` + - `*.taxonomy.rpt`: [Taxonomy report](https://github.com/ncbi/fcs/wiki/FCS-GX-taxonomy-report#taxonomy-report-output-). + - `*.fcs_gx_report.txt`: A final report of [recommended actions](https://github.com/ncbi/fcs/wiki/FCS-GX#outputs). + - `*.inter.tax.rpt.tsv`: [Select columns](../modules/local/ncbi_fcs_gx_krona_plot.nf) from `*.taxonomy.rpt` used for generation of a Krona taxonomy plot. + - `*.fcs.gx.krona.cut`: Krona taxonomy file [created](../modules/local/ncbi_fcs_gx_krona_plot.nf) from `*.inter.tax.rpt.tsv`. + - `*.fcs.gx.krona.html`: Krona taxonomy plot. + +
    + +[FCS-GX detects](https://github.com/ncbi/fcs/wiki/FCS-GX#outputs) contamination from foreign organisms in genome sequences. + ### BUSCO
    @@ -155,6 +166,8 @@ LTR Assembly Index (LAI) is a reference-free genome metric that [evaluates assem Hi-C contact mapping experiments measure the frequency of physical contact between loci in the genome. The resulting dataset, called a “contact map,” is represented using a [two-dimensional heatmap](https://github.com/igvteam/juicebox.js) where the intensity of each pixel indicates the frequency of contact between a pair of loci. +
    AssemblyQC - HiC interactive contact map
    AssemblyQC - HiC interactive contact map
    + ### Synteny
    @@ -166,7 +179,7 @@ Hi-C contact mapping experiments measure the frequency of physical contact betwe - `bundled.links.tsv`: Bundled links file generated with MUMMER and `dnadiff.pl`. - `circos.conf`: CIRCOS configuration file used to generate the synteny plot. - `karyotype.tsv`: Karyotype TSV file used to generate the synteny plot. - - `*.on.*.*`: Synteny files corresponding to of a single contig of the target assembly with respect to all contig of the reference assembly. + - `*.on.*.*`: Synteny files corresponding to a single contig of the target assembly with respect to all contigs of the reference assembly.
    Synteny plots are created with Circos which is a tool [facilitating](https://circos.ca) the identification and analysis of similarities and differences arising from comparisons of genomes. The genome-wide alignments are performed with [MUMMER](https://github.com/mummer4/mummer?tab=readme-ov-file) and bundled with [`dnadiff.pl`](https://github.com/mummer4/mummer/blob/master/scripts/dnadiff.pl). diff --git a/modules/local/generatekaryotype.nf b/modules/local/generatekaryotype.nf index c4ddfb2d..0135b8a1 100644 --- a/modules/local/generatekaryotype.nf +++ b/modules/local/generatekaryotype.nf @@ -20,8 +20,8 @@ process GENERATEKARYOTYPE { """ cat <<-END_VERSIONS > versions.yml "${task.process}": - awk: \$(awk --version | sed -n 's/awk version //p') - grep: \$(grep --version | sed -n 's/grep (BSD grep, GNU compatible) //p') + awk: \$(awk -W version | sed -n 's/mawk //p') + grep: \$(grep --version | sed -n '/grep (GNU grep) /s/grep //p') sed: \$(sed --version | sed -n 's/^sed //p') END_VERSIONS diff --git a/modules/local/splitbundlefile.nf b/modules/local/splitbundlefile.nf index 3fff1496..093c21f4 100644 --- a/modules/local/splitbundlefile.nf +++ b/modules/local/splitbundlefile.nf @@ -36,7 +36,7 @@ process SPLITBUNDLEFILE { cat <<-END_VERSIONS > versions.yml "${task.process}": - awk: \$(awk --version | sed -n 's/awk version //p') + awk: \$(awk -W version | sed -n 's/mawk //p') END_VERSIONS """ } From 36f102f97b8733c3065d2fac5e7b49dc7b36482b Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 4 Mar 2024 12:54:37 +1300 Subject: [PATCH 62/65] Updated usage doc --- .../templates/kraken2/kraken2.html | 3 +-- docs/images/kraken2.jpg | Bin 0 -> 129208 bytes docs/output.md | 21 +++++++++++++++--- docs/usage.md | 15 ++++++------- 4 files changed, 26 insertions(+), 13 deletions(-) create mode 100644 docs/images/kraken2.jpg diff --git a/bin/report_modules/templates/kraken2/kraken2.html b/bin/report_modules/templates/kraken2/kraken2.html index 522df1d8..250eebf3 100644 --- a/bin/report_modules/templates/kraken2/kraken2.html +++ b/bin/report_modules/templates/kraken2/kraken2.html @@ -1,8 +1,7 @@ diff --git a/docs/output.md b/docs/output.md index c838c3a1..b215f5c6 100644 --- a/docs/output.md +++ b/docs/output.md @@ -150,7 +150,7 @@ LTR Assembly Index (LAI) is a reference-free genome metric that [evaluates assem
    -Kraken2 [assigns taxonomic labels](https://ccb.jhu.edu/software/kraken2/) to sequencing reads for metagenomics projects. +Kraken2 [assigns taxonomic labels](https://ccb.jhu.edu/software/kraken2/) to sequencing reads for metagenomics projects. Further reading regarding performance of Kraken2:
    AssemblyQC - Interactive Krona plot from Kraken2 taxonomy
    AssemblyQC - Interactive Krona plot from Kraken2 taxonomy
    @@ -203,7 +203,7 @@ Synteny plots are created with Circos which is a tool [facilitating](https://cir ### Pipeline information -
    +
    Output files - `pipeline_info/` From edfb5766a2d3555a5866e6ce06b762e5034269e1 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 4 Mar 2024 13:36:47 +1300 Subject: [PATCH 64/65] Bumped version to 1.4 --- CITATION.cff | 2 +- nextflow.config | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/CITATION.cff b/CITATION.cff index bc1311fa..efb1b209 100644 --- a/CITATION.cff +++ b/CITATION.cff @@ -21,7 +21,7 @@ authors: - family-names: "Deng" given-names: "Cecilia" title: "AssemblyQC: A NextFlow pipeline for evaluating assembly quality" -version: 1.4dev +version: 1.4 date-released: 2024-02-12 url: "https://github.com/Plant-Food-Research-Open/assembly_qc" doi: 10.5281/zenodo.10647870 diff --git a/nextflow.config b/nextflow.config index 0910481d..f0ee1b5b 100644 --- a/nextflow.config +++ b/nextflow.config @@ -262,7 +262,7 @@ manifest { description = """A NextFlow pipeline which evaluates assembly quality with multiple QC tools and presents the results in a unified html report.""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '1.4dev' + version = '1.4' doi = '10.5281/zenodo.10647870' } From 5f403db02a157fb152a6bb07884eca47cf8679f6 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 4 Mar 2024 14:40:48 +1300 Subject: [PATCH 65/65] Updated CHANGELOG --- CHANGELOG.md | 26 +++++++++++++++++--------- version_check.sh | 2 +- 2 files changed, 18 insertions(+), 10 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 6680321b..dc2e51c8 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## 1.4dev - [28-Feb-2024] +## 1.4 - [04-Mar-2024] ### `Added` @@ -17,21 +17,29 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 8. Replaced custom GUNZIP module with nf-core/gunzip 9. Replaced custom gt/stat with pfr/gt/stat 10. Replaced custom fasta_validator with nf-core/fastavalidator -11. Removed BIOCODE GFF3 STATS owing to its frequent failures -12. Added pre-commit version checking -13. Now gt/stat reports extended stats and multiple distribution plots have been added to the report -14. Added a tools tab to the report which lists the tools used by the pipeline to create the report -15. Refactored and cleaned data flows for all the custom sub-workflow -16. Started using nf-core template -17. Started using semantic versioning -18. Moved all python depending packages to 'docker.io/gallvp/python3npkgs:v0.6' +11. Added pre-commit version checking +12. Now gt/stat reports extended stats and multiple distribution plots have been added to the report +13. Added a tools tab to the report which lists the tools used by the pipeline to create the report +14. Refactored and cleaned data flows for all the custom sub-workflow +15. Started using nf-core template +16. Started using semantic versioning +17. Moved all python depending packages to 'docker.io/gallvp/python3npkgs:v0.6' ### `Fixed` +1. All modules are now emitting versioning information +2. Fixed a bug which caused LAI to run with null assembly fasta +3. Fixed FASTA_LTRRETRIEVER_LAI sub-workflow so that it respects `monoploid_ids` parameter. + ### `Dependencies` +1. NextFlow!>=23.04.0 +2. nf-validation@1.1.3 + ### `Deprecated` +1. Removed BIOCODE GFF3 STATS owing to its frequent failures + ## v1.3 [08-Feb-2023] 1. Docker engine is now also supported diff --git a/version_check.sh b/version_check.sh index 04a714f7..e23860d3 100755 --- a/version_check.sh +++ b/version_check.sh @@ -10,5 +10,5 @@ fi # Check CHANGELOG version -grep "## $config_version" CHANGELOG.md >/dev/null \ +grep "## $config_version - " CHANGELOG.md >/dev/null \ || (echo 'Failed to match CHANGELOG version'; exit 1)

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    \n" + for (param in group_params.keySet()) { + summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "