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MD5 FAIL #18

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yangguang8112 opened this issue Mar 10, 2020 · 7 comments
Open

MD5 FAIL #18

yangguang8112 opened this issue Mar 10, 2020 · 7 comments

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@yangguang8112
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Hello, I recently found some problems using repaq-0.3.0.
After decompressing, I found that the md5 check failed. Comparing the decompressed fastq file with the original file, it was found that at some point in some reads N became G after being compressed. Is this a machine problem or an algorithm problem? I compressed 160 files and 20 of them were problematic. Recompressing these 20 is still the same result.
Here is the result of the --compare parameter
"result":"failed", "msg":"The RFQ file and FASTQ file have different sequence in the 7815 pair. GGACCTCTTCTGACTGATGGGAAATCACAGCAGTTGGAGACCCAGGTCCACAGGAAGGATGAAGAACCCAAGGAATGGCAGCAGACTGAGAGCTTCTGGA | GGACCTCTTCTGACTGATGGGAAATCACAGCAGTTGGAGACCCAGGTCCACAGGAAGGATGAAGAACCCAAGNAATGGCAGCAGACTGAGAGCTTCTGGA"

@sfchen
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sfchen commented Mar 10, 2020

Are you using the latest version?

If yes, can you upload a small file that can reproduce this issue?

@sfchen
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sfchen commented Mar 16, 2020

Can you please try the latest version v0.4.0 ?

@yangguang8112
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Can you please try the latest version v0.4.0 ?

Sorry, I was a bit busy last week, I will try the 0.4.0 version this week

@sfchen
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sfchen commented Mar 16, 2020

thank you. If it fails again, please help to upload a small file to reproduce this issue.

@yangguang8112
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test1.fq.gz
test2.fq.gz
The same error occurred in the latest version, the above files can reproduce this problem.

@bartns
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bartns commented Aug 27, 2020

Any update on this? I can reproduce this issue with the given test files.

repaq -c -i test1.fq.gz -I test2.fq.gz -o testGZ.rfq.xz
repaq --compare --in1 test1.fq.gz --in2 test2.fq.gz -r testGZ.rfq.xz                                                                                                        
{
	"result":"failed",
	"msg":"The RFQ file and FASTQ file have different sequence in the 7815 pair. GGACCTCTTCTGACTGATGGGAAATCACAGCAGTTGGAGACCCAGGTCCACAGGAAGGATGAAGAACCCAAGGAATGGCAGCAGACTGAGAGCTTCTGGA | GGACCTCTTCTGACTGATGGGAAATCACAGCAGTTGGAGACCCAGGTCCACAGGAAGGATGAAGAACCCAAGNAATGGCAGCAGACTGAGAGCTTCTGGA",
	"fastq_reads":15630,
	"rfq_reads":15630,
	"fastq_bases":1563000,
	"rfq_bases":1563000
}

BTW, I cloned the git repository. (v0.4.0 is not in the releases)

@genetalks-fred
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I used v0.4.0, also the same error occurred, any updates on this question?

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