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<!DOCTYPE html>
<html lang="en">
<head>
<meta charset="UTF-8">
<title>Metabolomics Workbench File Validator</title>
<link rel="stylesheet" href="styles/styles.css"/>
</head>
<body class="background">
<div>
<h1><a href="https://MoseleyBioinformaticsLab.github.io/mwFileStatusWebsite/" style="text-decoration:none;color:white;">Metabolomics Workbench File Validator</a></h1>
<p>
Last Updated: 2024-12-01 07:09:26.824246
</p>
</div>
<div>
<h2>Statistics</h2>
<p>
Number of Studies: 2<br>
Number of Analyses: 3<br>
</p>
<h3>Validation Statistics</h3>
<div class="stats__grid">
<div class="stats__grid__item">Status</div>
<div class="stats__grid__item">mwTab</div>
<div class="stats__grid__item">JSON</div>
<a href="https://MoseleyBioinformaticsLab.github.io/mwFileStatusWebsite/passing" class="stats__grid__item brightgreen">Passing</a>
<div class="stats__grid__item">0</div>
<div class="stats__grid__item">0</div>
<a href="https://MoseleyBioinformaticsLab.github.io/mwFileStatusWebsite/parsing_error" class="stats__grid__item red">Parsing Error</a>
<div class="stats__grid__item">0</div>
<div class="stats__grid__item">0</div>
<a href="https://MoseleyBioinformaticsLab.github.io/mwFileStatusWebsite/validation_error" class="stats__grid__item orange">Validation Error</a>
<div class="stats__grid__item">0</div>
<div class="stats__grid__item">0</div>
<a href="https://MoseleyBioinformaticsLab.github.io/mwFileStatusWebsite/missing" class="stats__grid__item lightgrey">Missing</a>
<div class="stats__grid__item">3</div>
<div class="stats__grid__item">3</div>
</div>
<h3>Comparison Statistics</h3>
<div class="compare__stats__grid">
<div class="stats__grid__item">Status</div>
<div class="stats__grid__item">Count</div>
<div class="stats__grid__item brightgreen">Consistent</div>
<div class="stats__grid__item">0</div>
<div class="stats__grid__item orange">Inconsistent</div>
<div class="stats__grid__item">0</div>
<div class="stats__grid__item lightgrey">Not Checked</div>
<div class="stats__grid__item">3</div>
</div>
</div>
<div>
<h2>File Status</h2>
<div class="grid header">
<a href="#ST003113" class="study__grid__item">ST003113: Inhibition of Asparagine Synthetase Effectively Retards Polycystic Kidney Disease Progression, investigated with targeted tracing metabolomics analysis in MEF cells using 15N2-glutamine. - San Raffaele University - Stefanoni, Davide</a>
<div class="grid__description" style="height:8em;max-height:8">
<div class="desc__grid">
<div class="desc__grid__item">STUDY_TITLE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Inhibition of Asparagine Synthetase Effectively Retards Polycystic Kidney Disease Progression, investigated with targeted tracing metabolomics analysis in MEF cells using 15N2-glutamine.</div>
<div class="desc__grid__item">STUDY_SUMMARY</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Polycystic Kidney Disease (PKD) is a genetic disorder characterized by bilateral cyst formation. We showed that PKD cells and kidneys display metabolic alterations, including the Warburg effect and glutaminolysis, sustained in vitro by the enzyme asparagine synthetase (ASNS). Here, we used antisense oligonucleotides (ASO) against Asns in orthologous and slowly progressive PKD murine models and show that treatment leads to a drastic reduction of total kidney volume (measured by MRI) and a prominent rescue of renal function in the mouse. Mechanistically, the upregulation of an ATF4-ASNS axis in PKD is driven by the amino acid response (AAR) branch of the integrated stress response (ISR). Metabolic profiling of PKD or control kidneys treated with Asns-ASO or Scr-ASO revealed major changes in the mutants, several of which are rescued by Asns silencing in vivo. Indeed, ASNS drives glutamine-dependent de novo pyrimidine synthesis and proliferation in cystic epithelia. Notably, while several metabolic pathways were completely corrected by Asns-ASO, glycolysis was only partially restored. Accordingly, combining the glycolytic inhibitor 2DG with Asns-ASO further improved efficacy. Our studies identify a new therapeutic target and novel metabolic vulnerabilities in PKD. Of interest, in these tracing studies we could confirm that the pyrimidine biosynthesis pathway is increased and rescued by silencing of Asns.</div>
<div class="desc__grid__item">INSTITUTE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">San Raffaele University</div>
<div class="desc__grid__item">LAST_NAME</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Stefanoni</div>
<div class="desc__grid__item">FIRST_NAME</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Davide</div>
<div class="desc__grid__item">ADDRESS</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Via Olgettina 58, Milan, Milan, 20132, Italy</div>
<div class="desc__grid__item">EMAIL</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">stefanoni.davide@hsr.it</div>
<div class="desc__grid__item">PHONE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">+393337686005</div>
</div>
</div>
</div>
<div class="grid">
<a href="#AN005098" class="analysis__grid__item lightgrey">AN005098</a>
<div class="grid__description">
<div>
<span>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005098/mwtab/txt" target="_blank">txt</a>
</div>
<div class="shieldRight lightgrey">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/docs/validation_logs/AN005098_txt.log" target="_blank">Missing/Blank</a>
</div>
</div>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005098/mwtab/json" target="_blank">json</a>
</div>
<div class="shieldRight lightgrey">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/docs/validation_logs/AN005098_json.log" target="_blank">Missing/Blank</a>
</div>
</div>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005098/mwtab/comparison" target="_blank">comparison</a>
</div>
<div class="shieldRight lightgrey">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/docs/validation_logs/AN005098_comparison.log" target="_blank">Not Checked</a>
</div>
</div>
</span>
</div>
<br>
<div class="desc__grid">
<div class="desc__grid__item">ANALYSIS_ID</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">AN005098</div>
</div>
</div>
<a href="#AN005099" class="analysis__grid__item lightgrey">AN005099</a>
<div class="grid__description">
<div>
<span>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005099/mwtab/txt" target="_blank">txt</a>
</div>
<div class="shieldRight lightgrey">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/docs/validation_logs/AN005099_txt.log" target="_blank">Missing/Blank</a>
</div>
</div>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005099/mwtab/json" target="_blank">json</a>
</div>
<div class="shieldRight lightgrey">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/docs/validation_logs/AN005099_json.log" target="_blank">Missing/Blank</a>
</div>
</div>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005099/mwtab/comparison" target="_blank">comparison</a>
</div>
<div class="shieldRight lightgrey">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/docs/validation_logs/AN005099_comparison.log" target="_blank">Not Checked</a>
</div>
</div>
</span>
</div>
<br>
<div class="desc__grid">
<div class="desc__grid__item">ANALYSIS_ID</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">AN005099</div>
</div>
</div>
</div> <br> <div class="grid header">
<a href="#ST003389" class="study__grid__item">ST003389: Disruption of Glucose Homeostasis by Bacterial Infection Orchestrates Host Innate Immunity Through NAD+/NADH Balance - Northwest A&F University - Xu, Lei</a>
<div class="grid__description" style="height:8em;max-height:8">
<div class="desc__grid">
<div class="desc__grid__item">STUDY_TITLE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Disruption of Glucose Homeostasis by Bacterial Infection Orchestrates Host Innate Immunity Through NAD+/NADH Balance</div>
<div class="desc__grid__item">STUDY_SUMMARY</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Metabolic reprogramming is crucial for activating innate immunity in macrophages, and the accumulation of immunometabolites is thought essential for effective defense against infection. The NAD+/NADH redox couple serves as a critical node that integrates metabolic pathways and signaling events, but how this metabolite couple engages macrophage activation remains unclear. Here, we showed that the NAD+/NADH ratio serves as a molecular signal that regulates proinflammatory responses and type I interferon (IFN) responses divergently. Salmonella Typhimurium infection led to a decreased NAD+/NADH ratio by inducing the accumulation of NADH. Further investigation showed that an increased NAD+/NADH ratio correlates to attenuated proinflammatory responses and enhanced type I IFN responses. Conversely, a decreased NAD+/NADH ratio is linked to intensified proinflammatory responses and restrained type I IFN responses. These results showed the NAD+/NADH ratio is an essential cell-intrinsic factor that orchestrates innate immunity, which enhanced our understanding of how metabolites fine-tune innate immunity.</div>
<div class="desc__grid__item">INSTITUTE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Northwest A&F University</div>
<div class="desc__grid__item">LAST_NAME</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Xu</div>
<div class="desc__grid__item">FIRST_NAME</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Lei</div>
<div class="desc__grid__item">ADDRESS</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Yangling, Xinong Road, Xiyang, 712100, China</div>
<div class="desc__grid__item">EMAIL</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">xulei@nwafu.edu.cn</div>
<div class="desc__grid__item">PHONE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">18149321513</div>
</div>
</div>
</div>
<div class="grid">
<a href="#AN005557" class="analysis__grid__item lightgrey">AN005557</a>
<div class="grid__description">
<div>
<span>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005557/mwtab/txt" target="_blank">txt</a>
</div>
<div class="shieldRight lightgrey">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/docs/validation_logs/AN005557_txt.log" target="_blank">Missing/Blank</a>
</div>
</div>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005557/mwtab/json" target="_blank">json</a>
</div>
<div class="shieldRight lightgrey">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/docs/validation_logs/AN005557_json.log" target="_blank">Missing/Blank</a>
</div>
</div>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005557/mwtab/comparison" target="_blank">comparison</a>
</div>
<div class="shieldRight lightgrey">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/docs/validation_logs/AN005557_comparison.log" target="_blank">Not Checked</a>
</div>
</div>
</span>
</div>
<br>
<div class="desc__grid">
<div class="desc__grid__item">ANALYSIS_ID</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">AN005557</div>
</div>
</div>
</div> <br> </div>
</body>
</html>