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I use a totally the same fq, with the same sequence and quality, but only the read name differ. And got a different algnment result, here is one example of a read:
As you see, two reads with the same sequence and quality:
CCACAAAATAATAACAGCCATCTATGACAGACCCACAGCCAACATCATACAGAATGGGGAAAAATTGGAGGCATA
EEEEEEEEEEEEEEEEEEEEE@EEEEEEEEEEEEEEE@EEEEEEEEEEEEEEEEEEEEEEEEEEEEE@6EEEEEE..............................
but only with different read name:
2305151010:9:2D240611017US2S2720DX:L00:R001C020:2179:0810
202408121835_2305151010_2D240611017US2S2720DX_B_20240812B86L00R001C020:21790810:9
while have different results:
the first one is mapped:2305151010:9:2D240611017US2S2720DX:L00:R001C020:2179:0810
the second is unmap: 202408121835_2305151010_2D240611017US2S2720DX_B_20240812B86L00R001C020:21790810:9
the version of bowtie2 I used is 2.5.1, I cant see any update or changes about read name from the newly versions, and maybe the new version may have same siuation too.
Looking forward to it, thanks!
The text was updated successfully, but these errors were encountered:
I use a totally the same fq, with the same sequence and quality, but only the read name differ. And got a different algnment result, here is one example of a read:
As you see, two reads with the same sequence and quality:
CCACAAAATAATAACAGCCATCTATGACAGACCCACAGCCAACATCATACAGAATGGGGAAAAATTGGAGGCATA
EEEEEEEEEEEEEEEEEEEEE@EEEEEEEEEEEEEEE@EEEEEEEEEEEEEEEEEEEEEEEEEEEEE@6EEEEEE..............................
but only with different read name:
2305151010:9:2D240611017US2S2720DX:L00:R001C020:2179:0810
202408121835_2305151010_2D240611017US2S2720DX_B_20240812B86L00R001C020:21790810:9
while have different results:
the first one is mapped:2305151010:9:2D240611017US2S2720DX:L00:R001C020:2179:0810
the second is unmap: 202408121835_2305151010_2D240611017US2S2720DX_B_20240812B86L00R001C020:21790810:9
the version of bowtie2 I used is 2.5.1, I cant see any update or changes about read name from the newly versions, and maybe the new version may have same siuation too.
Looking forward to it, thanks!
The text was updated successfully, but these errors were encountered: